A proliferation medium compounding device

Abstract

The present application relates to the technical field of Scirpus mariqueter culture equipment, and discloses a proliferation culture medium preparation device, which comprises a body mechanism, a powder feeding mechanism, a stirring mechanism and a reagent adding mechanism; the body mechanism comprises a base, an arm support and a hydraulic cylinder are respectively arranged on the top of the base, a top plate is arranged at the top of the arm support, and a culture dish is arranged at the top of the piston rod of the hydraulic cylinder; the powder feeding mechanism comprises a powder bin, and the powder bin is arranged on the outer side of the arm support close to the top. The proliferation culture medium preparation device solves the problem that the types of culture medium are relatively large in the prior art, and there are certain differences in the survival environment of Scirpus mariqueter, and the growth regulator needs to be adjusted accordingly. In large-scale proliferation culture, the work of the staff is greatly consumed, and the amount of chemical substances is deviated, which affects the proliferation culture of Scirpus mariqueter.

Description

Technical Field

[0001] This invention relates to the technical field of *Scirpus triquetrum* cultivation equipment, specifically a device for preparing a proliferation culture medium. Background Technology

[0002] *Scirpus triqueter* is a perennial herbaceous plant mainly distributed in the Yangtze River estuary. It belongs to the genus *Scirpus* in the family Cyperaceae and produces a large number of seeds with high starch content and underground bulbs, which can provide a large amount of plant and animal food for wetland waterbirds. As the most critical primary producer in the wetland ecosystem, it plays an irreplaceable role. Due to environmental impacts, its seed germination rate has decreased. In order to protect the ecosystem, the rapid and large-scale propagation of *Scirpus triqueter* through tissue culture technology is one of the effective methods and approaches to protect its germplasm resources.

[0003] To study the propagation and cultivation of *Scirpus triquetrum*, it is necessary to use propagation media to carry growth regulators for observation and experimentation. To develop suitable growth regulators for *Scirpus triquetrum*, various pre-prepared culture media are required. However, existing technologies involve numerous types of culture media, each with its own specific environmental variations, necessitating targeted formulation of growth regulators. Large-scale propagation and cultivation can be extremely labor-intensive, leading to deviations in chemical dosage and negatively impacting the propagation and cultivation of *Scirpus triquetrum*. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention provides a proliferation culture medium preparation device, which solves the problems mentioned in the background.

[0005] This invention provides the following technical solution: a proliferation culture medium preparation device, comprising: a body structure, a powder feeding mechanism, a stirring mechanism, and a reagent adding mechanism;

[0006] The machine body includes a base, an arm support and a hydraulic cylinder are respectively installed on the top of the base, a top plate is installed on the top of the arm support, and a petri dish is provided on the top of the piston rod of the hydraulic cylinder.

[0007] The powder feeding mechanism includes a powder bin, which is installed on the outer side of the arm support near the top. A guide hopper is installed on the inner side of the arm support near the top. The end of the guide hopper away from the arm support is located above the petri dish. A pushing mechanism is provided through the side of the powder bin near the bottom. The end of the pushing mechanism that passes through the powder bin and the arm support is located above the guide hopper.

[0008] The stirring mechanism includes a mixing mechanism, which is mounted on the top plate and positioned directly above the culture dish.

[0009] The reagent adding mechanism includes a liquid pressing rack, which is vertically pressed onto the top plate. A reagent rack is installed on the side of the top plate, and a reagent bottle is attached to the top of the reagent rack. A dispensing mechanism is installed at the bottom of the top plate, and a flexible tube is installed at the bottom of the dispensing mechanism. The bottom end of the reagent bottle passes through the reagent rack and is connected to the end of the flexible tube away from the dispensing mechanism.

[0010] Preferably, the pushing mechanism includes a pushing cylinder, a through groove is provided on the side of the powder hopper near the bottom, the through groove passes through the powder hopper and the arm support respectively, the pushing cylinder is slidably connected to the inner wall of the through groove, a material loading hopper is provided inside the pushing cylinder, a metering plug is slidably disposed inside the material loading hopper, an end cap is installed at the end of the pushing cylinder, a lead screw is installed at the end of the metering plug, the end of the lead screw away from the metering plug passes through the end cap and is equipped with a rotating handle, and the lead screw is threadedly connected to the end cap.

[0011] Preferably, the pusher cylinder has a feed inlet, the position and length of which correspond to the position of the powder hopper and the width of its inner wall, respectively. The side of the inner wall of the powder hopper is configured as a guide slope, and the bottom of the guide slope is located above the feed inlet.

[0012] Preferably, the mixing mechanism includes a motor, the output shaft of which is equipped with a stirring rod. The bottom end of the stirring rod passes through the top plate and is suspended inside the petri dish, and the stirring rod is located on the center line of the petri dish. A stirring blade is installed on the side of the stirring rod near the bottom end.

[0013] Preferably, a stage is mounted on the top of the hydraulic cylinder piston rod, and the surface of the stage is decorated with a central pattern, the diameter of which corresponds to the diameter of the culture dish.

[0014] Preferably, the liquid-pressing frame includes a pressure rod, both ends of which are fitted with positioning posts. The bottom end of the positioning post penetrates the top plate, and the positioning post is slidably connected to the top plate. A disc spring is fitted onto the surface of the positioning post, and the disc spring is located between the pressure rod and the top plate. A locking ring is fixedly fitted onto the surface of the positioning post, and the locking ring is located below the top plate. The bottom of the pressure rod corresponds to the top position of the injection mechanism.

[0015] Preferably, the injection mechanism includes a measuring cylinder with an external threaded interface near the top edge. A limiting sleeve is fixedly fitted below the external threaded interface. An installation cap is threaded onto the top of the measuring cylinder. A loading hole is formed on the surface of the top plate. The shape of the lower half of the inner wall of the loading hole matches the shape of the limiting sleeve. The shape of the upper half of the inner wall of the loading hole matches the shape of the bottom of the installation cap. A liquid pusher is slidably disposed on the inner wall of the measuring cylinder. A push-pull rod is installed on the top of the liquid pusher. The top end of the push-pull rod is installed on the liquid pressure frame.

[0016] Preferably, the measuring cylinder has an integrally formed liquid outlet on its side, and a liquid guide tube is installed at the outer end of the liquid outlet. The end of the liquid guide tube away from the liquid outlet is located above the petri dish. A slide cylinder is slidably sleeved on the inner wall of the bottom of the measuring cylinder. A blocking mechanism is installed on the top of the slide cylinder. The blocking mechanism is slidably connected to the inner wall of the measuring cylinder. A flexible tube is installed at the bottom of the slide cylinder. A quantitative resistive element is sleeved on the surface of the slide cylinder. The quantitative resistive element overlaps with the bottom of the measuring cylinder.

[0017] Preferably, the blocking mechanism includes a valve body, which is mounted on the top of the slide cylinder. A sealing sleeve is fitted around the outer edge of the valve body, and the outer edge of the sealing sleeve is slidably connected to the inner wall of the measuring cylinder. A liquid-blocking cavity is formed inside the valve body, a ball plug is attached to the bottom of the liquid-blocking cavity, a compression spring is attached to the top of the ball plug, and the top of the compression spring is attached to the top of the inner wall of the liquid-blocking cavity. A suction hole is formed at the top of the valve body, a push ring is attached to the bottom of the valve body, and a spring is installed at the bottom of the push ring. Both the push ring and the spring are slidably fitted onto the surface of the slide cylinder.

[0018] Preferably, the quantitative resistance element includes a collar, the top of which overlaps with the bottom of the measuring cylinder, a transition groove is provided at the bottom of the collar, a brake block is rotatably provided on the inner wall of the transition groove, the bottom end of the brake block is in the shape of an arc wedge, a stop ring is threadedly connected to the surface of the collar, the stop ring is slidably sleeved on the slide cylinder, and the bottom of the inner wall of the stop ring corresponds to the shape of the bottom of the brake block.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] 1. This culture medium preparation device, by setting up a main body mechanism, a powder feeding mechanism, a stirring mechanism, and a reagent adding mechanism, can quantitatively add pre-set powder and liquid reagents when preparing culture medium, and then use the stirring mechanism to mix them evenly, so that the culture medium can be prepared in a short time. This facilitates the large-scale preparation of culture medium, eliminating the need to weigh each reagent individually, thereby reducing the mental effort required by staff and preventing deviations in the dosage of chemical substances.

[0021] 2. This proliferation culture medium preparation device, by setting up a powder hopper, a pushing mechanism, and a guide hopper, adjusts the loading space inside the pushing mechanism according to the ratio of the proliferation culture medium reagents to load the powder reagents in a fixed ratio. Then, the loaded powder is poured out by pushing and rotating, and the powder is introduced into the culture dish by the guide hopper, thereby achieving the effect of rapid preparation of powder reagents. It is suitable for the unified preparation of large batches of culture dishes without weighing each one individually, reducing the time and effort spent on large-scale preparation.

[0022] 3. This culture medium preparation device, by setting up a reagent rack, reagent bottles, a liquid pressing rack, and a dispensing mechanism, places different liquid reagents required for preparation on the reagent rack and connects it to the dispensing mechanism. Then, by adjusting the capacity of the dispensing mechanism and pressing the liquid pressing rack, the liquid reagents of different proportions drawn up in the dispensing mechanism are uniformly dispensed, thereby achieving the effect of rapid preparation of liquid reagents.

[0023] 4. This proliferation culture medium preparation device, by setting up a hydraulic cylinder, a petri dish and a mixing mechanism, allows for the preparation of proliferation culture medium by first setting the ratio of powdered reagents and liquid reagents, then placing an empty petri dish on top of the hydraulic cylinder, which lifts it horizontally to below the mixing mechanism, and then simultaneously preparing and mixing various reagents, thereby improving the preparation efficiency of proliferation culture medium. Attached Figure Description

[0024] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0025] Figure 2 This is a schematic diagram of the side cross-section structure of the present invention;

[0026] Figure 3 For the present invention Figure 2 Enlarged structural diagram at point A in the middle;

[0027] Figure 4 This is a schematic diagram of the material feeding mechanism of the present invention in the material discharge state.

[0028] Figure 5 This is a schematic diagram of the cross-sectional structure of the powder storage compartment of the present invention;

[0029] Figure 6 This is a schematic diagram of the liquid pressure frame structure of the present invention;

[0030] Figure 7 This is a schematic diagram of the reagent rack structure of the present invention;

[0031] Figure 8 This is a schematic diagram of the side cross-section of the loading hole structure of the present invention;

[0032] Figure 9 This is a side sectional view of the injection mechanism of the present invention;

[0033] Figure 10 For the present invention Figure 9 Enlarged structural diagram at point B;

[0034] Figure 11 This is a schematic diagram of the disassembled structure of the quantitative resistive component of the present invention.

[0035] In the diagram: 1. Base; 2. Arm support; 3. Top plate; 4. Hydraulic cylinder; 5. Petri dish; 6. Mixing mechanism; 61. Motor; 62. Stirring rod; 63. Stirring blade; 64. Platform; 7. Powder hopper; 8. Pushing mechanism; 81. Push cylinder; 82. Through groove; 83. Loading bin; 84. Inlet; 85. Metering stopper; 86. End cap; 87. Lead screw; 88. Rotary handle; 9. Guide hopper; 10. Reagent rack; 11. Reagent bottle; 12. Liquid clamping rack; 121. Pressure rod; 122. Positioning post; 123. Disc spring; 124. Locking ring; 13 131. Injection mechanism; 132. Measuring cylinder; 133. Limiting sleeve; 134. Push plug; 135. Push-pull rod; 136. Mounting cap; 137. Liquid outlet; 138. Slide cylinder; 1371. Valve body; 1372. Sealing sleeve; 1373. Liquid blocking chamber; 1374. Compression spring; 1375. Ball plug; 1376. Liquid suction hole; 138. Metering stop; 1381. Collar; 1382. Adapter groove; 1383. Brake block; 139. Brake ring; 14. Hoses; 15. Loading hole; 16. Liquid guide tube; 17. Spring; 18. Push ring. Detailed Implementation

[0036] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0037] Please see Figure 1-11 A culture medium preparation device includes: a body structure, a powder feeding mechanism, a stirring mechanism, and a reagent adding mechanism;

[0038] The machine body includes a base 1, an arm support 2 and a hydraulic cylinder 4 are respectively installed on the top of the base 1, a top plate 3 is installed on the top of the arm support 2, and a petri dish 5 is provided on the top of the piston rod of the hydraulic cylinder 4.

[0039] The powder feeding mechanism includes a powder bin 7, which is installed on the outer side of the arm support 2 near the top. A guide hopper 9 is installed on the inner side of the arm support 2 near the top. The end of the guide hopper 9 away from the arm support 2 is located above the petri dish 5. A pushing mechanism 8 is provided through the side of the powder bin 7 near the bottom. The end of the pushing mechanism 8 that passes through the powder bin 7 and the arm support 2 is located above the guide hopper 9.

[0040] The stirring mechanism includes a mixing mechanism 6, which is mounted on the top plate 3 and positioned directly above the culture dish 5.

[0041] The reagent adding mechanism includes a liquid pressing rack 12, which is vertically pressed onto the top plate 3. A reagent rack 10 is installed on the side of the top plate 3, and a reagent bottle 11 is attached to the top of the reagent rack 10. A dispensing mechanism 13 is installed at the bottom of the top plate 3, and a flexible tube 14 is installed at the bottom of the dispensing mechanism 13. The bottom end of the reagent bottle 11 passes through the reagent rack 10 and is connected to the end of the flexible tube 14 away from the dispensing mechanism 13. By setting up the body mechanism, powder feeding mechanism, stirring mechanism, and reagent adding mechanism, the pre-set powder reagents and liquid reagents can be added quantitatively when preparing the proliferation culture medium. Then, the stirring mechanism is used to mix them evenly, so that the proliferation culture medium can be prepared in a short time. This facilitates the large-scale preparation of proliferation culture medium without having to weigh each reagent individually, thereby reducing the effort required by the staff during preparation and preventing deviations in the dosage of chemical substances.

[0042] The feeding mechanism 8 includes a feeding cylinder 81. A through groove 82 is provided on the side of the powder hopper 7 near the bottom. The through groove 82 passes through the powder hopper 7 and the arm support 2. The feeding cylinder 81 is slidably connected to the inner wall of the through groove 82. A material loading hopper 83 is provided inside the feeding cylinder 81. A metering plug 85 is slidably disposed inside the material loading hopper 83. An end cap 86 is installed at the end of the feeding cylinder 81. A lead screw 87 is installed at the end of the metering plug 85. The end of the lead screw 87 away from the metering plug 85 passes through the end cap 86 and is equipped with a rotating handle 88. The lead screw 87 is threadedly connected to the end cap 86. By setting the feeding mechanism 8, the size of the loading space inside the feeding mechanism 8 can be adjusted so that it can load powder reagents according to a fixed ratio, thereby achieving the effect of rapid preparation of powder reagents.

[0043] The pusher cylinder 81 is provided with a feed inlet 84. The position and length of the feed inlet 84 correspond to the position and width of the inner wall of the powder hopper 7, respectively. The side of the inner wall of the powder hopper 7 is set as a guide slope, and the bottom of the guide slope is located above the feed inlet 84. After the powder reagent is loaded into the powder hopper 7, the powder will directly enter the feed inlet 84 under the guidance of its internal structure, thereby improving the accuracy of the powder reagent dosage and avoiding the situation where there are dead corners inside, resulting in insufficient dosage.

[0044] The mixing mechanism 6 includes a motor 61, and a stirring rod 62 is mounted on the output shaft of the motor 61. The bottom end of the stirring rod 62 passes through the top plate 3 and is suspended inside the culture dish 5. The stirring rod 62 is located on the center line of the culture dish 5. A stirring blade 63 is mounted on the side of the stirring rod 62 near the bottom end. The culture dish 5 is lifted horizontally to the bottom of the mixing mechanism 6 by the hydraulic cylinder 4, and then various reagents are prepared and mixed at the same time, thereby improving the preparation efficiency of the proliferation culture medium.

[0045] The hydraulic cylinder 4 has a platform 64 mounted on top of its piston rod. The surface of the platform 64 is decorated with centered patterns, the diameter of which corresponds to the diameter of the culture dish 5. By decorating the platform 64 with centered patterns, the culture dish 5 can be placed with a strong sense of purpose, thus ensuring that it tends to be in the center position after being raised and lowered, and avoiding the situation where the mixing mechanism 6 collides with the inner wall of the culture dish 5 when it rotates.

[0046] The liquid pressing frame 12 includes a pressing rod 121, with positioning posts 122 sleeved at both ends of the pressing rod 121. The bottom end of the positioning post 122 penetrates the top plate 3, and the positioning post 122 is slidably connected to the top plate 3. A disc spring 123 is sleeved on the surface of the positioning post 122, and the disc spring 123 is located between the pressing rod 121 and the top plate 3. A locking ring 124 is fixedly sleeved on the surface of the positioning post 122, and the locking ring 124 is located below the top plate 3. The bottom of the pressing rod 121 corresponds to the top position of the injection mechanism 13. By setting the liquid pressing frame 12, multiple injection mechanisms 13 are connected into one unit, so that they can be injected uniformly, thereby ensuring the accuracy of the liquid reagent ratio.

[0047] The filling mechanism 13 includes a measuring cylinder 131. The measuring cylinder 131 has an external threaded interface near its top edge. A limiting sleeve 132 is fixedly fitted below the external threaded interface. An installation cap 135 is threaded onto the top of the measuring cylinder 131. A loading hole 15 is formed on the surface of the top plate 3. The shape of the lower half of the inner wall of the loading hole 15 matches the shape of the limiting sleeve 132, and the shape of the upper half of the inner wall of the loading hole 15 matches the shape of the bottom of the installation cap 135. A push plug 133 is slidably disposed on the inner wall of the measuring cylinder 131. A push-pull rod 134 is installed on the top of the push plug 133. The top end of the push-pull rod 134 is mounted on the pressure frame 12. By setting the installation cap 135, the measuring cylinder 131 can be disassembled, facilitating cleaning of the inner wall of the measuring cylinder 131 after use and also facilitating the replacement of damaged measuring cylinders 131.

[0048] The measuring cylinder 131 has an integrally formed liquid outlet 136 on its side. A liquid guide tube 16 is installed at the outer end of the liquid outlet 136. The end of the liquid guide tube 16 away from the liquid outlet 136 is located above the culture dish 5. A sliding cylinder 137 is slidably sleeved on the inner wall of the bottom of the measuring cylinder 131. A blocking mechanism is installed on the top of the sliding cylinder 137. The blocking mechanism is slidably connected to the inner wall of the measuring cylinder 131. The bottom end of the sliding cylinder 137 is installed with a flexible tube 14. A quantitative blocking element 138 is sleeved on the surface of the sliding cylinder 137. The quantitative blocking element 138 overlaps with the bottom of the measuring cylinder 131. By using the blocking mechanism to block the liquid outlet 136, liquid leakage in the measuring cylinder 131 can be effectively avoided before the liquid is pushed, thereby ensuring the accuracy of the preparation ratio of the proliferation culture medium.

[0049] The blocking mechanism includes a valve body 1371, which is mounted on the top of a slide cylinder 137. A sealing sleeve 1372 is fitted around the outer edge of the valve body 1371, and the outer edge of the sealing sleeve 1372 is slidably connected to the inner wall of the measuring cylinder 131. A liquid-blocking cavity 1373 is formed inside the valve body 1371. A ball plug 1375 is attached to the bottom of the liquid-blocking cavity 1373, and a compression spring 1374 is attached to the top of the ball plug 1375. The top of the compression spring 1374 is attached to the top of the inner wall of the liquid-blocking cavity 1373. The top of the valve body 1371 is provided with a liquid suction hole 1376. The bottom of the valve body 1371 is connected to a push ring 18, and a spring 17 is installed at the bottom of the push ring 18. The push ring 18 and the spring 17 are slidably sleeved on the surface of the slide cylinder 137. The blocking mechanism is set as a one-way valve structure. When the push plug 133 rises with the liquid pressure frame 12, the blocking mechanism rises synchronously under the action of the spring 17 until the liquid outlet 136 is blocked. At this time, the measuring cylinder 131 will draw a certain amount of liquid reagent due to the air pressure, so as to achieve the effect of quantitative weighing and mixing.

[0050] The quantitative resistive element 138 includes a collar 1381, the top of which overlaps with the bottom of the measuring cylinder 131. A transition groove 1382 is formed at the bottom of the collar 1381, and a brake block 1383 is rotatably mounted on the inner wall of the transition groove 1382. The bottom end of the brake block 1383 is an arc-shaped wedge. A stop ring 139 is threaded onto the surface of the collar 1381. The stop ring 139 is slidably fitted onto the slide cylinder 137, and the stop ring 139... The bottom of the inner wall of the ring 139 corresponds to the shape of the bottom of the brake block 1383. By setting a quantitative stop 138, the length of the slide cylinder 137 is controlled by the quantitative stop 138, thereby adjusting the position of the blocking mechanism inside the measuring cylinder 131. When the quantitative stop 138 touches the bottom of the measuring cylinder 131, due to the air pressure of the measuring cylinder 131, the blocking mechanism cannot continue to rise. It can only draw the liquid reagent in the reagent bottle 11 to fill the pressure generated by the rise of the liquid pressure rack 12 in the measuring cylinder 131.

[0051] The working principle is as follows: When preparing the proliferation culture medium, the pushing mechanism 8 and the injection mechanism 13 are first adjusted according to the proportion of each reagent. Then, a certain amount of water is loaded into the culture dish 5, and the culture dish 5 is placed at the top center of the hydraulic cylinder 4. The hydraulic cylinder 4 is started, and the culture dish 5 is lifted to a suitable stirring position below the mixing mechanism 6. Then, the pushing mechanism 8 is pushed and rotated to pour the powdered reagents onto the guide hopper 9 in sequence, and the guide hopper 9 is used to guide the reagents into the culture dish 5. Then, the liquid pressing rack 12 is pressed, and multiple injection mechanisms 13 simultaneously start to output a certain amount of liquid reagents into the culture dish 5. Then, the mixing mechanism 6 is started to mix the reagents in the culture dish 5. After the preparation is completed, the hydraulic cylinder 4 is started to lower and remove the culture dish 5, and the preparation of the next batch of proliferation culture medium can begin.

[0052] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A device for preparing a growth culture medium, characterized in that, include: The main body, powder feeding mechanism, mixing mechanism, and reagent adding mechanism; The body structure includes a base (1), an arm support (2) and a hydraulic cylinder (4) are respectively installed on the top of the base (1), a top plate (3) is installed on the top of the arm support (2), and a petri dish (5) is provided on the top of the piston rod of the hydraulic cylinder (4). The powder feeding mechanism includes a powder bin (7), which is installed on the outer side of the arm support (2) near the top. A guide hopper (9) is installed on the inner side of the arm support (2) near the top. The end of the guide hopper (9) away from the arm support (2) is located above the petri dish (5). A pushing mechanism (8) is provided through the side of the powder bin (7) near the bottom. The end of the pushing mechanism (8) that passes through the powder bin (7) and the arm support (2) is located above the guide hopper (9). The stirring mechanism includes a mixing mechanism (6), which is mounted on the top plate (3) and is located directly above the petri dish (5). The reagent adding mechanism includes a liquid pressing rack (12), which is vertically pressed on the top plate (3). A reagent rack (10) is installed on the side of the top plate (3). A reagent bottle (11) is attached to the top of the reagent rack (10). A dispensing mechanism (13) is installed at the bottom of the top plate (3). A hose (14) is installed at the bottom of the dispensing mechanism (13). The bottom end of the reagent bottle (11) passes through the reagent rack (10) and is connected to the end of the hose (14) away from the dispensing mechanism (13). The injection mechanism (13) includes a measuring cylinder (131), a liquid pusher (133) is slidably provided on the inner wall of the measuring cylinder (131), a push-pull rod (134) is installed on the top of the liquid pusher (133), and the top of the push-pull rod (134) is installed on the liquid pressure frame (12). The measuring cylinder (131) has an integrally formed liquid outlet (136) on its side. A liquid guide tube (16) is installed at the outer end of the liquid outlet (136). The end of the liquid guide tube (16) away from the liquid outlet (136) is located above the petri dish (5). A slide cylinder (137) is slidably sleeved on the inner wall of the bottom end of the measuring cylinder (131). A blocking mechanism is installed on the top of the slide cylinder (137). The blocking mechanism is slidably connected to the inner wall of the measuring cylinder (131). The bottom end of the slide cylinder (137) is installed with a hose (14). A quantitative resistive element (138) is sleeved on the surface of the slide cylinder (137). The quantitative resistive element (138) overlaps with the bottom of the measuring cylinder (131). The blocking mechanism includes a valve body (1371), which is mounted on the top of the slide cylinder (137). A sealing sleeve (1372) is fitted around the outer edge of the valve body (1371). The outer edge of the sealing sleeve (1372) is slidably connected to the inner wall of the measuring cylinder (131). A liquid-blocking cavity (1373) is formed inside the valve body (1371). A ball plug (1375) is attached to the bottom of the liquid-blocking cavity (1373). A compression spring (1374) is attached to the top of the plug (1375), and the top of the compression spring (1374) is attached to the top of the inner wall of the liquid blocking cavity (1373). A liquid suction hole (1376) is opened on the top of the valve body (1371), and a push ring (18) is attached to the bottom of the valve body (1371). A spring (17) is installed on the bottom of the push ring (18), and the push ring (18) and the spring (17) are slidably sleeved on the surface of the slide cylinder (137).

2. The proliferation culture medium preparation device according to claim 1, characterized in that, The feeding mechanism (8) includes a pusher cylinder (81). A through groove (82) is provided on the side of the powder hopper (7) near the bottom. The through groove (82) passes through the powder hopper (7) and the arm support (2) respectively. The pusher cylinder (81) is slidably connected to the inner wall of the through groove (82). A material loading hopper (83) is provided inside the pusher cylinder (81). A metering plug (85) is slidably provided inside the material loading hopper (83). An end cap (86) is installed at the end of the pusher cylinder (81). A screw rod (87) is installed at the end of the metering plug (85). The end of the screw rod (87) away from the metering plug (85) passes through the end cap (86) and is equipped with a rotating handle (88). The screw rod (87) is threadedly connected to the end cap (86).

3. The proliferation culture medium preparation device according to claim 2, characterized in that, The push cylinder (81) is provided with a feed inlet (84). The position and length of the feed inlet (84) correspond to the position and width of the powder hopper (7) and the inner wall, respectively. The side of the inner wall of the powder hopper (7) is set as a guide slope, and the bottom of the guide slope is located above the feed inlet (84).

4. The proliferation culture medium preparation device according to claim 1, characterized in that, The mixing mechanism (6) includes a motor (61), and a stirring rod (62) is installed on the output shaft of the motor (61). The bottom end of the stirring rod (62) passes through the top plate (3) and is suspended inside the petri dish (5). The stirring rod (62) is located on the center line of the petri dish (5). A stirring blade (63) is installed on the side of the stirring rod (62) near the bottom end.

5. The proliferation culture medium preparation device according to claim 4, characterized in that, The top of the piston rod of the hydraulic cylinder (4) is equipped with a stage (64), and the surface of the stage (64) is decorated with a central pattern, the diameter of which corresponds to the diameter of the petri dish (5).

6. The proliferation culture medium preparation device according to claim 1, characterized in that, The liquid pressure frame (12) includes a pressure rod (121), both ends of which are fitted with positioning pins (122). The bottom end of the positioning pins (122) penetrates the top plate (3), and the positioning pins (122) and the top plate (3) are slidably connected. A disc spring (123) is fitted on the surface of the positioning pins (122), and the disc springs (123) are located between the pressure rod (121) and the top plate (3). A locking ring (124) is fixedly fitted on the surface of the positioning pins (122), and the locking ring (124) is located below the top plate (3). The bottom of the pressure rod (121) corresponds to the top position of the injection mechanism (13).

7. The proliferation culture medium preparation device according to claim 1, characterized in that, The measuring cylinder (131) has an external threaded interface near the top edge. A limiting sleeve (132) is fixedly sleeved below the external threaded interface. An installation cap (135) is threaded on the top of the measuring cylinder (131). A loading hole (15) is provided on the surface of the top plate (3). The shape of the lower half of the inner wall of the loading hole (15) is adapted to the shape of the limiting sleeve (132). The shape of the upper half of the inner wall of the loading hole (15) is adapted to the shape of the bottom of the installation cap (135).

8. The proliferation culture medium preparation device according to claim 7, characterized in that, The quantitative resistive element (138) includes a collar (1381), the top of which overlaps with the bottom of the measuring cylinder (131). A transition groove (1382) is provided at the bottom of the collar (1381). A brake block (1383) is rotatably provided on the inner wall of the transition groove (1382). The bottom end of the brake block (1383) is in the shape of an arc wedge. A stop ring (139) is threadedly connected to the surface of the collar (1381). The stop ring (139) is slidably sleeved on the slide cylinder (137), and the bottom of the inner wall of the stop ring (139) corresponds to the shape of the bottom of the brake block (1383).

Images