A pesticide composition and its application in reducing pesticide dosage and / or controlling corn borer

Abstract

The present application belongs to the technical field of biological pesticides, and particularly relates to a pesticide composition and application thereof in reducing pesticide dosage and / or controlling corn borer. The present application can not only reduce the dosage of chlorantraniliprole, but also show good control effect on sorghum and corn borer by mixing azadirachtin and chlorantraniliprole according to the corresponding volume ratio and then applying to crops. Therefore, the technical scheme provided by the present application can replace the application of pure chlorantraniliprole, and reduce the negative influence of chemical pesticides.

Description

Technical Field

[0001] This invention belongs to the technical field of biological pesticides, specifically relating to a pesticide composition and its application in reducing pesticide usage and / or controlling corn borers. Background Technology

[0002] Sorghum (Sorghum bicolor) is the world's fifth largest food crop, and together with millet, foxtail millet, and soybeans, it forms part of my country's traditional dryland farming ecological agricultural system. It has a long history of cultivation and wide applications. However, the Asian corn borer (Ostriniafurnacalis Guenee) is one of the major pests that reduce sorghum yield and quality. It bores into the leaves, stems, and ear stalks of sorghum, thereby reducing photosynthetic efficiency and exacerbating the lodging rate of sorghum stalks.

[0003] Although chlorantraniliprole has been widely used to control corn borers with significant results, long-term reliance on chemical pesticides often leads to pest resistance and negative impacts on food and environmental safety. Biological pesticides, on the other hand, often suffer from poor timeliness and require large dosages, thus failing to provide rapid and effective control during outbreaks. Therefore, current research focuses solely on using single chemical or biological pesticides to control corn borers, without reporting any methods that reduce chemical pesticide usage while maintaining control efficacy. Summary of the Invention

[0004] The purpose of this invention is to provide a pesticide composition and its application in reducing pesticide use and / or controlling corn borers. This biological composition is not only green and safe, but also has the effect of reducing pesticide use and controlling corn borers.

[0005] The present invention provides a pesticide composition comprising azadirachtin and chlorantraniliprole; wherein the volume ratio of azadirachtin to chlorantraniliprole is 600-1200:70-80.

[0006] Preferably, the azadirachtin has a volume percentage of 0.3% to 0.5%; and the chlorantraniliprole comprises a chlorantraniliprole suspension with an active ingredient concentration of 200 to 400 g / L.

[0007] The present invention provides the application of the pesticide composition described in the above technical solution in reducing pesticide usage and / or controlling corn borer.

[0008] Preferably, the corn borer includes the Asian corn borer.

[0009] Preferably, the pesticide is chlorantraniliprole suspension; the pesticide targets sorghum and / or corn.

[0010] This invention provides a method for reducing pesticide use and / or controlling corn borer, comprising: applying the pesticide composition described in the above technical solution to crops;

[0011] The application dosage of the pesticide composition is 670–1280 mL / hm. 2 .

[0012] Preferably, the crop includes sorghum and / or corn.

[0013] Preferably, the method of application includes foliar spraying.

[0014] Beneficial effects:

[0015] This invention provides a pesticide composition comprising azadirachtin and chlorantraniliprole; the volume ratio of azadirachtin to chlorantraniliprole is 600-1200:70-80. By mixing azadirachtin and chlorantraniliprole in the appropriate volume ratio, this invention not only reduces the amount of chlorantraniliprole required but also demonstrates good control effects on sorghum. Furthermore, this biological pesticide composition is green and safe and does not inhibit sorghum growth.

[0016] Based on the above-mentioned technical advantages, the present invention also provides a method for reducing pesticide use and / or controlling corn borers, comprising: applying the pesticide composition described in the above-mentioned technical solution to crops; wherein the application dosage of the pesticide composition is 670-1280 mL / hm. 2 Experiments show that, by adopting the technical solution provided by this invention, a good control effect against corn borers can be achieved while reducing the dosage of chlorantraniliprole by 50%. Therefore, the technical solution provided by this invention can replace the application of pure chlorantraniliprole, reducing the negative impacts of chemical pesticides. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the embodiments will be briefly described below.

[0018] Figure 1 The control effects of different experimental treatments in Example 1 and Comparative Examples 1-8 on the Asian corn borer were shown. Detailed Implementation

[0019] This invention provides a pesticide composition comprising azadirachtin and chlorantraniliprole; the volume ratio of azadirachtin to chlorantraniliprole is 600–1200:70–80. By mixing azadirachtin and chlorantraniliprole in the corresponding volume ratio, not only can the dosage of chlorantraniliprole be reduced, but it can also exhibit good control effects on sorghum.

[0020] In this invention, the volume ratio of azadirachtin to chlorantraniliprole is 600-1200:70-80, preferably 1200:75; the azadirachtin is preferably azadirachtin emulsifiable concentrate; the volume percentage of the azadirachtin emulsifiable concentrate is preferably 0.3%-0.5%, more preferably 0.3%; the chlorantraniliprole is preferably a chlorantraniliprole suspension; the effective component of the chlorantraniliprole suspension is preferably 200-400 g / L, more preferably 200 g / L. This invention does not have a specific limitation on the source of the azadirachtin and chlorantraniliprole; they can be purchased conventionally. In a specific embodiment of this invention, the azadirachtin emulsifiable concentrate was purchased from Chengdu Green Gold Biotechnology Co., Ltd., and the chlorantraniliprole suspension was manufactured by FMC Corporation of the United States, and repackaged by Suzhou FMC Plant Protection Co., Ltd.

[0021] This invention provides a method for reducing pesticide use and / or controlling corn borers, comprising: applying the pesticide composition described in the above technical solution to crops; wherein the application dosage of the pesticide composition is 670–1280 mL / hm. 2 .

[0022] In this invention, azadirachtin and chlorantraniliprole are preferably mixed. In this invention, there are no special requirements for the mixing, as long as it is uniformly mixed; the azadirachtin is preferably azadirachtin emulsifiable concentrate; the chlorantraniliprole is preferably a chlorantraniliprole suspension; the volume percentage of the azadirachtin emulsifiable concentrate, the active ingredient of the chlorantraniliprole suspension, and the mixing volume ratio have been described in detail above and will not be repeated here.

[0023] After mixing, the pesticide composition is applied to crops according to the present invention. In this invention, the crop preferably includes sorghum and / or corn; more preferably sorghum; the application method is preferably foliar spraying; the application dosage of the pesticide composition during spraying is 670–1280 mL / hm². 2 More preferably 1275 mL / hm 2 When applying the pesticide composition to plants, the present invention preferably dilutes the pesticide composition before application; the solvent used for dilution is preferably water; the amount of water used is preferably sufficient to meet the planting area of ​​the crop while ensuring the application dosage of the pesticide composition. For example, the application dosage of the pesticide composition is 1275 mL / hm. 2 Furthermore, a solution volume of 750L is sufficient to meet the needs of 1hm 2 To meet the application requirements of sorghum, when preparing the pesticide composition, azadirachtin and chlorantraniliprole were directly mixed at a volume ratio of 1200 mL: 75 mL, and then diluted with water to 750 L to obtain a diluted solution containing the pesticide composition for related experiments. In a specific embodiment of the present invention, since the area of ​​the pot is only 0.2 m²... 2Therefore, the proportions and volumetric dosage of the pesticide composition can be calculated by adjusting the area of ​​the pot to be sprayed. Applying an appropriate amount of the pesticide composition can not only achieve good control of corn borers but also reduce the negative impacts of chemical pesticides.

[0024] This invention also provides the application of the pesticide composition described above in reducing pesticide usage and / or controlling corn borers. The reduction in pesticide usage is preferably achieved by reducing the amount of chlorantraniliprole suspension used; the target of the chlorantraniliprole suspension is preferably sorghum and / or corn, more preferably sorghum.

[0025] Experiments show that, by adopting the technical solution provided by this invention, a good control effect against corn borers can be achieved while reducing the dosage of chlorantraniliprole by 50%. Therefore, the technical solution provided by this invention can replace the application of pure chlorantraniliprole, while simultaneously controlling corn borers.

[0026] To further illustrate the present invention, the following detailed description, in conjunction with the accompanying drawings and embodiments, describes a pesticide composition provided by the present invention and its application in reducing pesticide usage and / or controlling corn borers, but these descriptions should not be construed as limiting the scope of protection of the present invention.

[0027] Experimental Preparation: From April to May 2022, a pot experiment of sorghum was conducted in the experimental greenhouse of the Suqian Agricultural Research Institute of Jiangsu Academy of Agricultural Sciences. The tested sorghum variety was "Yangmian 9". Sorghum seedlings were raised on April 3rd, and transplanted into pots on April 25th. Each pot contained 8 sorghum plants (one pot represented one treatment, with 4 replicates per treatment; a total of 8 treatments and 32 pots were used). Two-year-old corn borers of uniform morphology and size were inoculated onto the leaves of each sorghum plant. After the plants reached a stable state, 2 corn borers were retained per plant, and the pots were covered with 40-mesh netting. The area of ​​each pot was recorded as 0.2 m². 2 The field application rate is 750 L / hm. 2 After conversion, 15 mL of the pesticide to be sprayed should be applied to each pot.

[0028] Example 1

[0029] A method for reducing pesticide use and / or controlling corn borers, comprising the following steps:

[0030] 1) Test reagents: Chemical pesticide: Chlorantraniliprole (200g / L suspension concentrate) was manufactured by FMC Corporation, USA; Biological pesticide: Azadirachtin (0.3% emulsifiable concentrate by volume) was purchased from Chengdu Green Gold Biotechnology Co., Ltd.

[0031] 2) The tested corn borer:

[0032] The Asian corn borer eggs were provided by Henan Jiyuan Baiyun Industrial Co., Ltd. and were free from any pesticide exposure. Indoor rearing conditions included: 14h day / 10h night light hours and a temperature of 25±1℃ until the 2nd-3rd instar larvae were hatched. Feed was provided by Henan Jiyuan Baiyun Industrial Co., Ltd.

[0033] 3) Preparation of pesticide composition: Take 0.8 mL of azadirachtin and 0.05 mL of chlorantraniliprole from step 1) and add water to make up to 500 mL to obtain the pesticide to be sprayed; equivalent to mixing azadirachtin and chlorantraniliprole in a volume ratio of 1200 mL: 75 mL and then making up to 750 L with water to obtain the pesticide to be sprayed.

[0034] 4) Determine the insect control efficacy using the quantitative spraying method: Fill the sprayer with the pesticide to be sprayed in step 3) and spray 15 mL evenly into each pot of a treatment.

[0035] Comparative Example 1

[0036] The difference from Example 1 is that in step 3), 0.05 mL of chlorantraniliprole from step 1) is directly taken and diluted with water to 500 mL to obtain the agent to be sprayed; this is equivalent to taking 75 mL of chlorantraniliprole and diluting it with water to 750 L to obtain the agent to be sprayed.

[0037] Comparative Example 2

[0038] The difference from Comparative Example 1 is that in step 3), 0.1 mL of chlorantraniliprole from step 1) is directly taken and diluted with water to 500 mL to obtain the agent to be sprayed; this is equivalent to taking 150 mL of chlorantraniliprole and diluting it with water to 750 L to obtain the agent to be sprayed.

[0039] Comparative Example 3

[0040] The difference from Example 1 is that in step 3), 0.6 mL of azadirachtin from step 1) is directly taken and diluted with water to 500 mL to obtain the agent to be sprayed; this is equivalent to taking 900 mL of azadirachtin and diluting it with water to 750 L to obtain the agent to be sprayed.

[0041] Comparative Example 4

[0042] The difference from Comparative Example 3 is that in step 3), 0.8 mL of azadirachtin from step 1) is directly taken and diluted with water to 500 mL to obtain the agent to be sprayed; this is equivalent to taking 1200 mL of azadirachtin and diluting it with water to 750 L to obtain the agent to be sprayed.

[0043] Comparative Example 5

[0044] The difference from Example 1 is that in step 3), azadirachtin and chlorantraniliprole are mixed at a volume ratio of 0.4 mL: 0.05 mL and then diluted with water to 500 mL to obtain the agent to be sprayed; this is equivalent to mixing azadirachtin and chlorantraniliprole at a volume ratio of 600 mL: 75 mL and then diluting with water to 750 L to obtain the agent to be sprayed.

[0045] Comparative Example 6

[0046] The difference from Example 1 is that in step 3), azadirachtin and chlorantraniliprole are mixed at a volume ratio of 0.6 mL: 0.05 mL and then water is added to make up to 500 mL; this is equivalent to mixing azadirachtin and chlorantraniliprole at a volume ratio of 900 mL: 75 mL and then water is added to make up to 750 L to obtain the agent to be sprayed.

[0047] Comparative Example 7

[0048] The difference from Example 1 is that step 3 is omitted;

[0049] In step 4): Fill a spray bottle with clean water and spray 15mL evenly into each basin of a treatment.

[0050] Results and Analysis

[0051] Statistical analysis was performed using Excel 2019 and SPSS 25.0 software, and graphs were generated using Origin 2018 software. All data were tested for normality and homogeneity of variance before analysis. One-way ANOVA and the Tukey-HSD method were used to compare the differences in insect control efficacy and sorghum plant growth inhibition rate among different treatments. In this invention, P < 0.05 was set as the statistical significance level.

[0052] 1) Statistical analysis was performed on Example 1 and Comparative Examples 1 to 7. Surveys were conducted on days 1, 3, and 7 after spraying to count the number of dead insects in each pot. The insect control efficacy was calculated based on the control treatment. Specific results are shown in Table 1 and... Figure 1 .

[0053] Where: Insect control efficacy = (Control live insect rate – Treatment live insect rate) / Control live insect rate × 100%

[0054] Survival rate = (Number of surviving insects / Total number of insects) × 100%.

[0055] Table 1. Control effects of different experimental treatments on corn borer.

[0056]

[0057] Note: The values ​​in parentheses represent the standard error. The control live insect rate in the formula is the live insect rate of each treatment in Comparative Example 7. The treatment live insect rate is the live insect rate of each treatment in Example 1 and Comparative Examples 1 to 6. The data shown in Table 1 are the average live insect rate of the control and the average live insect rate of the treatment.

[0058] There are table 1 and Figure 1 It can be seen that although chemical pesticides have faster and better insecticidal capabilities than biological pesticides, the combined use of biological and chemical pesticides can achieve the same effect. One day after application, 150 mL / hm² 2 The optimal dosage of chlorantraniliprole showed the best insecticidal effect (65.63%). Although the insecticidal effect of chlorantraniliprole at half the dosage (48.44%) decreased, it was still higher than that of azadirachtin. Higher dosages of azadirachtin (75 mL / hm) were also observed. 2 The dosage of chlorantraniliprole mixed with other pesticides can significantly improve the insect control effect, and 1200 mL / hm 2 Dosage of azadirachtin and 75 mL / hm 2 The amount of chlorantraniliprole mixed (56.25%) with 150 mL / hm 2 The insecticidal effect of chlorantraniliprole was comparable at certain dosages. Three days after application, 150 mL / hm²... 2 The dosage of chlorantraniliprole still showed the strongest insecticidal efficacy (66.70%), with three azadirachtin-chlorantraniliprole combinations (600+75, 900+75, and 1200+75 mL / hm). 2 The insect control efficacy showed no significant difference at 1200 + 75 mL / hm 2 The combination of azadirachtin and chlorantraniliprole with 150 mL / hm 2 There was no significant difference in the insecticidal efficacy of chlorantraniliprole; the insecticidal effects were similar. Seven days after application, at 150 mL / hm²... 2 The highest insecticidal efficacy was observed at concentrations of chlorantraniliprole (69.93%), but concentrations of 900+75 mL / hm² and 1200+75 mL / hm² were also effective. 2 The dosage of the azadirachtin-chlorantraniliprole combination was 150 mL / hm. 2 There was no significant difference in insect control efficacy between different dosages of chlorantraniliprole.

[0059] 2) Statistical analysis was performed on Example 1 and Comparative Examples 1 to 7 respectively. Specifically, 8 sorghum plants with similar growth conditions were selected from each treatment 14 days after spraying to measure plant height and calculate plant height inhibition rate. On the 1st, 7th and 14th day after spraying, 20 sorghum plants were selected from each treatment to investigate whether the plants showed symptoms of pesticide damage such as yellowing, wilting and deformity and the number of affected plants. The specific results are shown in Table 2.

[0060] Wherein: plant height growth inhibition rate = (control plant height growth rate – treatment plant height growth rate) / control plant height growth rate × 100%;

[0061] Plant height growth rate = (Plant height after application – Plant height before application) / Plant height before application × 100%.

[0062] Table 2 Effects of different experimental treatments on the growth of sorghum plants

[0063]

[0064] Note: The values ​​in parentheses represent the standard error. The plant height for control in the formula is the same as the plant height in Comparative Example 7, and the plant height for treatment is the same as the plant height in Example 1 and Comparative Examples 1 to 6.

[0065] Table 2 shows that both azadirachtin and chlorantraniliprole, whether used alone or in combination, inhibited sorghum growth to some extent. Chlorantraniliprole (75 and 150 mL / hm²) showed the highest inhibition rates. 2 ), azadirachtin (900 and 1200 mL / hm) 2 ) and azadirachtin-chlorantraniliprole combinations (600+75, 900+75 and 1200+75 mL / hm) 2 The seven treatments inhibited the growth of "Yangmian 9" by 10.46%, 12.06%, 9.03%, 10.50%, 11.23%, 10.55% and 12.07%, respectively, but the degree of influence was extremely low, and none of them were significantly different from control group 7.

[0066] In summary, 0.3% azadirachtin (900 mL / hm) 2 )+200g / L chlorantraniliprole (75mL / hm) 2 ) and 0.3% azadirachtin (1200 mL / hm) 2 )+200g / L chlorantraniliprole (75mL / hm) 2 All of these can achieve a concentration of 200 g / L chlorantraniliprole (150 mL / hm) 7 days after application. 2 It has considerable insect control efficacy, but 0.3% azadirachtin (1200 mL / hm) 2 )+200g / L chlorantraniliprole (75mL / hm) 2 ) and 200 g / L chlorantraniliprole (150 mL / hm 2The control efficacy against corn borers showed no significant difference at 1, 3, and 7 days after application. This means that when the application dose of azadirachtin is the recommended field dose, its combination with a halved dose of chlorantraniliprole provides higher insecticidal efficacy than azadirachtin alone and comparable to high doses of chlorantraniliprole alone. In other words, the azadirachtin-chlorantraniliprole combination can maintain good control of corn borers in sorghum and / or corn while reducing the amount of chlorantraniliprole required. Considering the time-sensitive requirements for corn borer control in agricultural production, a 0.3% azadirachtin (1200 mL / hm²) was used. 2 )+200g / L chlorantraniliprole (75mL / hm) 2 The best results are achieved when the technical solution provided by this invention is conducive to the sustainable development of green agriculture and ecological agriculture.

[0067] Although the above embodiments have provided a detailed description of the present invention, they are only some embodiments of the present invention, and not all embodiments. People can obtain other embodiments based on these embodiments without creative effort, and these embodiments all fall within the protection scope of the present invention.

Claims

1. The application of a pesticide composition in reducing pesticide usage and controlling corn borers, characterized in that, The pesticide composition consists of 0.3% azadirachtin emulsifiable concentrate and 200 g / L chlorantraniliprole suspension concentrate; the volume ratio of the 0.3% azadirachtin emulsifiable concentrate and the 200 g / L chlorantraniliprole suspension concentrate is 1200:70-80.

2. The application according to claim 1, characterized in that, The corn borer includes the Asian corn borer.

3. The application according to claim 1, characterized in that, The pesticide composition is applied to crops at a dosage of 670–1280 mL / hm². 2 .

4. The application according to claim 3, characterized in that, The types of crops mentioned include sorghum and / or corn.

5. The application according to claim 3 or 4, characterized in that, The application method is foliar spraying.

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