A chromatography experiment device and experiment method

Abstract

The present application relates to the technical field of experimental equipment, and particularly relates to a chromatography experimental device and an experimental method. In the chromatography experiment, by controlling the first valve structure, the second valve structure and the third valve structure, the first chromatography column and the second chromatography column can be in a series connection state, and then the liquid in the liquid inlet pipeline can pass through the first chromatography column and the second chromatography column in sequence. The first chromatography column can also be connected with the second bypass pipeline, so that the liquid in the liquid inlet pipeline can pass through the first chromatography column and the second bypass pipeline in sequence. The second chromatography column can also be connected with the first bypass pipeline, and the first chromatography column and the second chromatography column in series connection can be processed simultaneously in the operations of alkaline disinfection, pre-equilibrium, pre-loading equilibrium, regeneration, alkaline disinfection, equilibrium and preservation. Compared with the alkaline disinfection, pre-equilibrium, pre-loading equilibrium, regeneration, alkaline disinfection, equilibrium and preservation operations on the first chromatography column and the second chromatography column respectively, the experimental time is saved, and the experimental efficiency is improved.

Description

Technical Field

[0001] This invention relates to the field of experimental equipment technology, specifically to a chromatography experimental apparatus and method. Background Technology

[0002] Chromatographic processes typically include steps such as alkali sterilization, pre-equilibration, pre-loading equilibration, sample loading, post-loading equilibration, elution, regeneration, alkali sterilization, equilibration, and storage. Except for sample loading, post-equilibration, and elution, the other steps are identical. In current chromatographic experiments, a single chromatographic apparatus is usually configured with one inlet line, performing each step individually at a time. When using two or more columns, each column must be chromatographically analyzed separately, resulting in longer experimental times and lower chromatographic efficiency. Summary of the Invention

[0003] This invention provides a chromatography experimental apparatus and method to improve the technical problem of low chromatography efficiency in current chromatography experimental apparatuses.

[0004] In a first aspect, one embodiment provides a chromatography experimental apparatus, including a liquid inlet line, a first chromatography column, a second chromatography column, and a first series line, wherein the first series line is used to connect the first chromatography column and the second chromatography column in series, and further includes a first bypass line connected in parallel with the first chromatography column and a second bypass line connected in parallel with the second chromatography column; the chromatography experimental apparatus includes a first valve structure, a second valve structure, and a third valve structure;

[0005] Both ends of the first bypass pipeline are connected to the first valve structure and the second valve structure, respectively; both ends of the first chromatography column are connected to the first valve structure and the second valve structure, respectively; the first valve structure is connected to the inlet pipeline and is used to selectively allow the liquid in the inlet pipeline to enter the first chromatography column or the first bypass pipeline; the second valve structure is used to selectively allow the liquid flowing out of the first chromatography column or the liquid flowing out of the first bypass pipeline to enter the first series pipeline.

[0006] The third valve structure is located between the second valve structure and the second chromatography column, and the liquid inlet end of the second chromatography column is connected to the third valve structure; the two ends of the first series pipeline are respectively connected to the second valve structure and the third valve structure, and the liquid inlet end of the second bypass pipeline is connected to the third valve structure; the third valve structure is used to allow the liquid in the first series pipeline to selectively enter the second bypass pipeline or the second chromatography column.

[0007] Furthermore, in one embodiment, the chromatography apparatus includes a third chromatography column and a second series pipeline, the second series pipeline being used to connect the second chromatography column and the third chromatography column in series, and the chromatography apparatus also includes a third bypass pipeline connected in parallel with the third chromatography column; the chromatography apparatus includes a fourth valve structure and a fifth valve structure;

[0008] The fifth valve structure is located between the fourth valve structure and the third chromatography column, and the inlet end of the third chromatography column is connected to the fifth valve structure; the inlet end of the third bypass pipeline is connected to the fifth valve structure; the fourth valve structure is used to select whether the liquid flowing out of the second chromatography column or the liquid flowing out of the second bypass pipeline enters the second series pipeline; the fifth valve structure is used to select whether the liquid in the second series pipeline enters the third bypass pipeline or the third chromatography column.

[0009] In another embodiment, the number of the first chromatography columns is two or more, and the first chromatography columns are arranged in parallel.

[0010] Furthermore, in one embodiment, each of the first chromatography columns is connected to the first valve structure, which enables the liquid in the inlet pipeline to selectively enter any number of the first chromatography columns.

[0011] In a further embodiment, the first valve structure includes a main valve and a branch valve. The main valve is a three-way valve, and the branch valve is a shut-off valve. The branch valve corresponds one-to-one with the first chromatography column. The main valve is used to selectively allow the liquid in the inlet pipeline to enter either the first bypass pipeline or the branch valve. The branch valve is used to control the connection and disconnection between the first chromatography column corresponding to the branch valve and the main valve.

[0012] Furthermore, in one embodiment, at least one of the first valve structure, the second valve structure, and the third valve structure is a three-way valve.

[0013] Furthermore, in one embodiment, the three-way valve is an electrically controlled valve.

[0014] Furthermore, in one embodiment, the three-way valve is a manual valve.

[0015] Furthermore, in one embodiment, the number of the liquid inlet pipes is one.

[0016] In a second aspect, one embodiment provides a chromatography experimental method using the chromatography experimental apparatus described in any embodiment of the first aspect, comprising:

[0017] By controlling the first valve structure, the second valve structure and the third valve structure, the liquid in the inlet pipeline passes through the first chromatography column and the second chromatography column in series in sequence, and performs alkali disinfection, pre-equilibration and pre-loading equilibration operations on the first chromatography column and the second chromatography column.

[0018] By controlling the first valve structure, the second valve structure, and the third valve structure, the liquid in the inlet pipeline passes through the first bypass pipeline and the second bypass pipeline in sequence, and the first bypass pipeline and the second bypass pipeline are balanced. By controlling the first valve structure, the second valve structure, and the third valve structure, the liquid in the inlet pipeline passes through the first chromatography column and the second bypass pipeline in sequence, and the first chromatography column is subjected to sample loading, post-loading balancing, and elution operations in sequence.

[0019] By controlling the first valve structure, the second valve structure, and the third valve structure, the liquid in the inlet pipeline passes through the first bypass pipeline and the second bypass pipeline in sequence, and the first bypass pipeline and the second bypass pipeline are balanced. By controlling the first valve structure, the second valve structure, and the third valve structure, the liquid in the inlet pipeline passes through the first bypass pipeline and the second chromatography column in sequence, and the second chromatography column is subjected to sample loading, post-loading balancing, and elution operations in sequence.

[0020] By controlling the first valve structure, the second valve structure, and the third valve structure, the liquid in the inlet pipeline passes sequentially through the first and second chromatography columns connected in series, performing regeneration, alkali disinfection, balancing, and storage operations on the first and second chromatography columns in one operation.

[0021] According to the chromatography experimental apparatus and method described in the above embodiments, during the chromatography experiment, by controlling the first valve structure, the second valve structure, and the third valve structure, the first and second chromatography columns can be connected in series, allowing the liquid in the inlet line to pass through the first and second chromatography columns sequentially. Alternatively, the first chromatography column can be connected to a second bypass line, allowing the liquid in the inlet line to pass through the first and second chromatography columns sequentially, without passing through the second chromatography column. Conversely, the second chromatography column can be connected to a first bypass line, allowing the liquid in the inlet line to pass through the first bypass line and the second chromatography column sequentially. By switching the valve structures in the chromatography experimental apparatus, the first and second chromatography columns connected in series can be processed simultaneously during alkali disinfection, pre-equilibration, pre-loading equilibration, regeneration, alkali disinfection, equilibration, and storage operations. Compared to performing these operations separately on the first and second chromatography columns, this saves experimental time and improves experimental efficiency. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the chromatography experimental apparatus in one embodiment;

[0023] Figure 2This is a state diagram of the first chromatography column and the second bypass line in one embodiment (thick lines represent the lines through which the liquid is fed, and thin lines represent the lines through which the liquid is not fed).

[0024] Figure 3 This is a state diagram of the second chromatography column and the first bypass line in one embodiment (thick lines represent the lines through which the liquid is fed, and thin lines represent the lines through which the liquid is not fed).

[0025] Figure 4 This is a state diagram of the first and second chromatography columns in one embodiment when they are connected (thick lines represent pipelines through which the liquid is fed, and thin lines represent pipelines through which the liquid is not fed).

[0026] Figure 5 This is a schematic diagram of a chromatography apparatus having a third chromatography column in one embodiment.

[0027] Figure 6 This is a schematic diagram of a chromatography apparatus having two first chromatography columns in one embodiment.

[0028] The following is a list of feature names corresponding to the reference numerals in the figure: 1. Inlet line; 2. First chromatography column; 3. Second chromatography column; 4. First series line; 5. First bypass line; 6. Second bypass line; 7. First valve structure; 71. Main valve; 72. Branch valve; 8. Second valve structure; 9. Third valve structure; 10. Third chromatography column; 11. Second series line; 12. Third bypass line; 13. Fourth valve structure; 14. Fifth valve structure. Detailed Implementation

[0029] The present invention will now be described in further detail with reference to specific embodiments and accompanying drawings. Similar elements in different embodiments are referred to by associated similar element reference numerals. In the following embodiments, many details are described to facilitate a better understanding of this application. However, those skilled in the art will readily recognize that some features may be omitted in different situations, or may be replaced by other elements, materials, or methods. In some cases, certain operations related to this application are not shown or described in the specification. This is to avoid obscuring the core parts of this application with excessive description. For those skilled in the art, detailed description of these related operations is not necessary; they can fully understand the related operations based on the description in the specification and general technical knowledge in the art.

[0030] Furthermore, the features, operations, or characteristics described in the specification can be combined in any suitable manner to form various embodiments. At the same time, the steps or actions in the method description can be rearranged or adjusted in a manner obvious to those skilled in the art. Therefore, the various orders in the specification and drawings are only for the clear description of a particular embodiment and do not imply a necessary order, unless otherwise stated that a particular order must be followed.

[0031] The serial numbers assigned to components in this document, such as "first" and "second," are used only to distinguish the described objects and have no sequential or technical meaning. The terms "connection" and "linkage" used in this application, unless otherwise specified, include both direct and indirect connections (linkages).

[0032] Current chromatography apparatuses typically have a single inlet line. This means that when at least two chromatography columns are required, they can usually only be operated separately. To improve experimental efficiency, this application provides a new chromatography apparatus. By controlling the valve structure of the chromatography apparatus, the connection state of the inlet line, the first chromatography column, and the second chromatography column can be changed, thereby allowing the first and second chromatography columns to be processed simultaneously in the same processing steps, saving experimental time.

[0033] In one embodiment, please refer to Figures 1 to 6 The chromatography apparatus includes an inlet line 1, a first chromatography column 2, a second chromatography column 3, a first series line 4, a first bypass line 5 connected in parallel with the first chromatography column 2, a second bypass line 6 connected in parallel with the second chromatography column 3, a first valve structure 7, a second valve structure 8, and a third valve structure 9. The first series line 4 connects the first chromatography column 2 and the second chromatography column 3 in series. The two ends of the first bypass line 5 are connected to the first valve structure 7 and the second valve structure 8, respectively. The two ends of the first chromatography column 2 are also connected to the first valve structure 7 and the second valve structure 8, respectively. The first valve structure 7 is connected to the inlet line 1 and allows the liquid from the inlet line 1 to selectively enter either the first chromatography column 2 or the first bypass line 5. The second valve structure 8 allows the liquid flowing from either the first chromatography column 2 or the first bypass line 5 to selectively enter the first series line 4.

[0034] The third valve structure 9 is located between the second valve structure 8 and the second chromatography column 3, with the inlet end of the second chromatography column 3 connected to the third valve structure 9. The two ends of the first series pipeline 4 are connected to the second valve structure 8 and the third valve structure 9, respectively, and the inlet end of the second bypass pipeline 6 is connected to the third valve structure 9. The third valve structure 9 is used to selectively allow the liquid from the first series pipeline 4 to enter either the second bypass pipeline 6 or the second chromatography column 3.

[0035] The first valve structure 7, the second valve structure 8, and the third valve structure 9 each have at least two states.

[0036] Please refer to Figure 3 One state of the first valve structure 7 is: connecting the first bypass line 5 to the inlet line 1 and disconnecting the inlet line 1 from the first chromatography column 2; please refer to Figure 2 Another state is: the inlet pipe 1 is connected to the first chromatography column 2 and the first bypass pipe 5 is disconnected from the inlet pipe 1.

[0037] Please refer to Figure 2 One state of the second valve structure 8 is: connecting the first series pipeline 4 to the first chromatography column 2 and disconnecting the first series pipeline 4 from the first bypass pipeline 5; please refer to Figure 3 Another state is: the first series pipeline 4 is connected to the first bypass pipeline 5 column and the first series pipeline 4 is disconnected from the first chromatography column 2.

[0038] Please refer to Figure 2 One state of the third valve structure 9 is: connecting the second bypass line 6 to the first series line 4 and disconnecting the first series line 4 from the second chromatography column 3; please refer to Figure 3 Another state is: the first series pipeline 4 is connected to the second chromatography column 3 and the second bypass pipeline 6 is disconnected from the first series pipeline 4.

[0039] The chromatography apparatus has a series configuration (please refer to...). Figure 4 ), First standalone mode (please refer to) Figure 2 ) and the second separate mode (please refer to) Figure 3 In the series mode, the first chromatography column 2 and the second chromatography column 3 are connected in series, and the liquid in the inlet line 1 can pass through the first chromatography column 2 and the second chromatography column 3 in sequence, without passing through the first bypass line 5 and the second bypass line 6. In the first standalone mode, the liquid in the inlet line 1 can pass through the first chromatography column 2 and the second bypass line 6 in sequence, without passing through the second chromatography column 3 and the first bypass line 5. In the second standalone mode, the liquid in the inlet line 1 can pass through the first bypass line 5 and the second chromatography column 3 in sequence, without passing through the first chromatography column 2 and the second bypass line 6.

[0040] The chromatography process includes alkali sterilization, pre-equilibration, pre-loading equilibration, sample loading, post-loading equilibration, elution, regeneration, alkali sterilization, equilibration, and preservation. The steps involving sample loading onto the first chromatography column 2 and the second chromatography column 3, such as sample loading, post-loading equilibration, and elution, require separate processing for different samples. In the pre-loading preparation steps, such as alkali sterilization, pre-equilibration, and pre-loading equilibration, the treatment steps for the first chromatography column 2 and the second chromatography column 3 are the same; therefore, the chromatography apparatus can be used in series. Similarly, in the subsequent processing steps of regeneration, alkali sterilization, equilibration, and preservation, the treatment steps for the first chromatography column 2 and the second chromatography column 3 are also the same; therefore, the chromatography apparatus also uses a series configuration.

[0041] During chromatography experiments, by switching the working mode of the chromatography apparatus, the apparatus can be in series mode during alkali sterilization, pre-equilibration, pre-loading equilibration, regeneration, alkali sterilization, equilibration, and storage operations, simultaneously processing the first chromatography column 2 and the second chromatography column 3 in series. The first separate mode is used when processing samples from the first chromatography column 2, and the second separate mode is used when processing samples from the second chromatography column 3. Compared to performing the entire experimental operation on the first chromatography column 2 and the second chromatography column 3 separately, the chromatography apparatus of this application can save experimental time and improve experimental efficiency.

[0042] Specifically, in one embodiment, please refer to Figures 1 to 4 The chromatography experimental methods of the chromatography experimental apparatus include:

[0043] By controlling the first valve structure 7, the second valve structure 8 and the third valve structure 9, the liquid in the inlet pipeline 1 passes through the first chromatography column 2 and the second chromatography column 3 connected in series in sequence, and performs alkali disinfection, pre-equilibration and pre-loading equilibration operations on the first chromatography column 2 and the second chromatography column 3.

[0044] By controlling the first valve structure 7, the second valve structure 8, and the third valve structure 9, the liquid in the inlet pipe 1 passes through the first bypass pipe 5 and the second bypass pipe 6 in sequence, and the first bypass pipe 5 and the second bypass pipe 6 are balanced. By controlling the first valve structure 7, the second valve structure 8, and the third valve structure 9, the liquid in the inlet pipe 1 passes through the first chromatography column 2 and the second bypass pipe 6 in sequence, and the first chromatography column 2 is loaded with samples, balanced after loading, and eluted in sequence.

[0045] By controlling the first valve structure 7, the second valve structure 8, and the third valve structure 9, the liquid in the inlet pipe 1 passes sequentially through the first bypass pipe 5 and the second bypass pipe 6, and the first bypass pipe 5 and the second bypass pipe 6 are balanced. By controlling the first valve structure 7, the second valve structure 8, and the third valve structure 9, the liquid in the inlet pipe 1 passes sequentially through the first bypass pipe 5 and the second chromatography column 3, and the second chromatography column 3 is subjected to sample loading, post-loading balancing, and elution operations sequentially.

[0046] By controlling the first valve structure 7, the second valve structure 8 and the third valve structure 9, the liquid in the inlet pipeline 1 passes sequentially through the first chromatography column 2 and the second chromatography column 3 connected in series, and performs regeneration, alkali disinfection, balancing and storage operations on the first chromatography column 2 and the second chromatography column 3 in one operation.

[0047] Furthermore, in one embodiment, please refer to Figures 1 to 4 The chromatography experimental apparatus includes a fourth valve structure 13, which is connected to the liquid outlet of the second chromatography column 3 and the liquid outlet of the second bypass pipeline 6.

[0048] Furthermore, in one embodiment, please refer to Figure 5 The chromatography apparatus includes a third chromatography column 10 and a second series conduit 11, which connects the second chromatography column 3 and the third chromatography column 10 in series. The apparatus also includes a third bypass conduit 12 connected in parallel with the third chromatography column 10. The apparatus includes a fifth valve structure 14, with one end of the second series conduit 11 connected to a fourth valve structure 13 and the other end connected to the fifth valve structure 14. The fourth valve structure 13 is used to select whether the liquid flowing from the second chromatography column 3 or the liquid flowing from the second bypass conduit 6 enters the second series conduit 11.

[0049] The fifth valve structure 14 is located between the fourth valve structure 13 and the third chromatography column 10, and the inlet end of the third chromatography column 10 is connected to the fifth valve structure 14. The inlet end of the third bypass line 12 is connected to the fifth valve structure 14, and the fifth valve structure 14 is used to selectively allow the liquid from the second series line 11 to enter either the third bypass line 12 or the third chromatography column 10.

[0050] The fourth valve structure 13 has at least two states, one of which is: connecting the second series line 11 to the second chromatography column 3 and disconnecting the second series line 11 from the second bypass line 6. The other state is: connecting the second series line 11 to the second bypass line 6 and disconnecting the second series line 11 from the second chromatography column 3.

[0051] One state of the fifth valve structure 14 is: connecting the third bypass line 12 with the second series line 11 and disconnecting the second series line 11 from the third chromatography column 10; another state is: connecting the second series line 11 with the third chromatography column 10 and disconnecting the third bypass line 12 from the second series line 11.

[0052] In series mode, the first chromatography column 2, the second chromatography column 3, and the third chromatography column 10 are connected in series. Liquid in the inlet line 1 can pass through the first chromatography column 2, the second chromatography column 3, and the third chromatography column 10 sequentially, without passing through the first bypass line 5, the second bypass line 6, and the third bypass line 12. In the first standalone mode, liquid in the inlet line 1 can pass through the first chromatography column 2, the second bypass line 6, and the third bypass line 12 sequentially, without passing through the second chromatography column 3, the third chromatography column 10, and the first bypass line 5. In the second standalone mode, liquid in the inlet line 1 can pass through the first bypass line 5, the second chromatography column 3, and the third bypass line 12 sequentially, without passing through the first chromatography column 2, the second bypass line 6, and the third chromatography column 10.

[0053] The chromatography apparatus has a third separate mode in which the liquid in the inlet line 1 can pass through the first bypass line 5, the second bypass line 6 and the third chromatography column 10 in sequence, without passing through the first chromatography column 2, the second chromatography column 3 and the third bypass line 12.

[0054] In one embodiment, please refer to Figure 6 To enrich the usage of the chromatography apparatus, two or more first chromatography columns 2 are used, arranged in parallel. This increases the sample throughput and processing speed in the first chromatography columns 2. Specifically, in one embodiment, please refer to... Figure 6 The number of first chromatography columns 2 is two. Of course, in some other embodiments, the number of first chromatography columns 2 and second chromatography columns 3 is one each. Similarly, in some other embodiments, the number of second chromatography columns may be more than two.

[0055] Furthermore, in one embodiment, please refer to Figure 6 Each first chromatography column 2 is connected to a first valve structure 7, which allows the liquid from the inlet line 1 to selectively enter any number of first chromatography columns 2. Specifically, in one embodiment, the first valve structure 7 includes a main valve 71 and a branch valve 72. The main valve 71 is a three-way valve, and the branch valve 72 is a shut-off valve. Each branch valve 72 corresponds to one of the first chromatography columns 2. The main valve 71 is used to selectively allow the liquid from the inlet line 1 to enter either the first bypass line 5 or the branch valve 72. The branch valve 72 is used to control the opening and closing of the pipeline between the first chromatography column 2 corresponding to the branch valve 72 and the main valve 71.

[0056] In one embodiment, please refer to Figures 1 to 6 At least one of the first valve structure 7, the second valve structure 8, and the third valve structure 9 is a three-way valve. Specifically, in one embodiment, the first valve structure 7, the second valve structure 8, and the third valve structure 9 are all three-way valves. In some other embodiments, the three-way valve can also be replaced by a three-way connector and two shut-off valves. The three ports of the first valve structure 7 are respectively connected to the inlet line 1, the first chromatography column 2, and the first bypass line 5; the three ports of the second valve structure 8 are respectively connected to the first chromatography column 2, the first bypass line 5, and the first series line 4; and the three ports of the third valve structure 9 are respectively connected to the second chromatography column 3, the first series line 4, and the second bypass line 6.

[0057] To facilitate control of the opening of the three-way valve, in one embodiment, the three-way valve is an electrically controlled valve. Of course, in another embodiment, the three-way valve can also be a manually operated valve. Manual valves are less expensive.

[0058] In one embodiment, please refer to Figures 1 to 6 The number of inlet pipes 1 is one.

[0059] For a chromatography experimental method using the chromatography experimental apparatus in any of the above embodiments, please refer to... Figures 1 to 6 ,include:

[0060] By controlling the first valve structure 7, the second valve structure 8 and the third valve structure 9, the liquid in the inlet pipeline 1 passes through the first chromatography column 2 and the second chromatography column 3 in series in sequence, and performs alkali disinfection, pre-equilibration and pre-loading equilibration operations on the first chromatography column 2 and the second chromatography column 3.

[0061] By controlling the first valve structure 7, the second valve structure 8, and the third valve structure 9, the liquid in the inlet pipe 1 passes sequentially through the first bypass pipe 5 and the second bypass pipe 6, and the first bypass pipe 5 and the second bypass pipe 6 are balanced. By controlling the first valve structure 7, the second valve structure 8, and the third valve structure 9, the liquid in the inlet pipe 1 passes sequentially through the first chromatography column 2 and the second bypass pipe 6, and the first chromatography column 2 is subjected to sample loading, post-loading balancing, and elution operations in sequence.

[0062] By controlling the first valve structure 7, the second valve structure 8, and the third valve structure 9, the liquid in the inlet pipe 1 passes sequentially through the first bypass pipe 5 and the second bypass pipe 6, and the first bypass pipe 5 and the second bypass pipe 6 are balanced. By controlling the first valve structure 7, the second valve structure 8, and the third valve structure 9, the liquid in the inlet pipe 1 passes sequentially through the first bypass pipe 5 and the second chromatography column 3, and the second chromatography column 3 is subjected to sample loading, post-loading balancing, and elution operations sequentially.

[0063] By controlling the first valve structure 7, the second valve structure 8 and the third valve structure 9, the liquid in the inlet pipeline 1 passes sequentially through the first chromatography column 2 and the second chromatography column 3 connected in series, and performs regeneration, alkali disinfection, balancing and storage operations on the first chromatography column 2 and the second chromatography column 3 in one operation.

[0064] The above examples illustrate the present invention only to aid in understanding it and are not intended to limit the scope of the invention. Those skilled in the art can make various simple deductions, modifications, or substitutions based on the principles of this invention.

Claims

1. A chromatographic experimental method using a chromatographic experimental apparatus, characterized in that, The chromatography experimental apparatus includes a liquid inlet line, a first chromatography column, a second chromatography column, and a first series line, wherein the first series line is used to connect the first chromatography column and the second chromatography column in series, and also includes a first bypass line connected in parallel with the first chromatography column and a second bypass line connected in parallel with the second chromatography column; the chromatography experimental apparatus includes a first valve structure, a second valve structure, and a third valve structure; The two ends of the first bypass pipeline are respectively connected to the first valve structure and the second valve structure, and the two ends of the first chromatography column are respectively connected to the first valve structure and the second valve structure. The first valve structure is connected to the inlet pipeline and is used to allow the liquid in the inlet pipeline to selectively enter the first chromatography column or the first bypass pipeline. The second valve structure is used to select whether the liquid flowing out of the first chromatography column or the liquid flowing out of the first bypass line enters the first series pipeline; The third valve structure is located between the second valve structure and the second chromatography column, and the liquid inlet end of the second chromatography column is connected to the third valve structure; the two ends of the first series pipeline are respectively connected to the second valve structure and the third valve structure, and the liquid inlet end of the second bypass pipeline is connected to the third valve structure; the third valve structure is used to allow the liquid in the first series pipeline to selectively enter the second bypass pipeline or the second chromatography column. The chromatography experimental method includes: By controlling the first valve structure, the second valve structure and the third valve structure, the liquid in the inlet pipeline passes through the first chromatography column and the second chromatography column in series in sequence, and performs alkali disinfection, pre-equilibration and pre-loading equilibration operations on the first chromatography column and the second chromatography column. By controlling the first valve structure, the second valve structure, and the third valve structure, the liquid in the inlet pipeline passes through the first bypass pipeline and the second bypass pipeline in sequence, and the first bypass pipeline and the second bypass pipeline are balanced. By controlling the first valve structure, the second valve structure, and the third valve structure, the liquid in the inlet pipeline passes through the first chromatography column and the second bypass pipeline in sequence, and the first chromatography column is subjected to sample loading, post-loading balancing, and elution operations in sequence. By controlling the first valve structure, the second valve structure, and the third valve structure, the liquid in the inlet pipeline passes through the first bypass pipeline and the second bypass pipeline in sequence, and the first bypass pipeline and the second bypass pipeline are balanced. By controlling the first valve structure, the second valve structure, and the third valve structure, the liquid in the inlet pipeline passes through the first bypass pipeline and the second chromatography column in sequence, and the second chromatography column is subjected to sample loading, post-loading balancing, and elution operations in sequence. By controlling the first valve structure, the second valve structure, and the third valve structure, the liquid in the inlet pipeline passes sequentially through the first and second chromatography columns connected in series, and the first and second chromatography columns are regenerated, disinfected with alkali, balanced, and stored sequentially.

2. The chromatography experimental method according to claim 1, characterized in that, The chromatography apparatus includes a third chromatography column and a second series pipeline, the second series pipeline being used to connect the second chromatography column and the third chromatography column in series. The chromatography apparatus also includes a third bypass pipeline connected in parallel with the third chromatography column. The chromatography apparatus includes a fourth valve structure and a fifth valve structure. The fifth valve structure is located between the fourth valve structure and the third chromatography column, and the inlet end of the third chromatography column is connected to the fifth valve structure; the inlet end of the third bypass pipeline is connected to the fifth valve structure; the fourth valve structure is used to select whether the liquid flowing out of the second chromatography column or the liquid flowing out of the second bypass pipeline enters the second series pipeline; the fifth valve structure is used to select whether the liquid in the second series pipeline enters the third bypass pipeline or the third chromatography column.

3. The chromatography experimental method according to claim 1, characterized in that, The number of the first chromatography columns is two or more, and the first chromatography columns are arranged in parallel.

4. The chromatography experimental method according to claim 2, characterized in that, Each of the first chromatography columns is connected to the first valve structure, which allows the liquid in the inlet line to selectively enter any number of the first chromatography columns.

5. The chromatography experimental method according to claim 3, characterized in that, The first valve structure includes a main valve and a branch valve. The main valve is a three-way valve, and the branch valve is a shut-off valve. Each branch valve corresponds to a first chromatography column. The main valve is used to allow the liquid in the inlet pipeline to selectively enter either the first bypass pipeline or the branch valve. The branch valve is used to control the connection between the first chromatography column corresponding to the branch valve and the main valve.

6. The chromatography experimental method according to any one of claims 1-5, characterized in that, At least one of the first valve structure, the second valve structure, and the third valve structure is a three-way valve.

7. The chromatography experimental method according to claim 6, characterized in that, The three-way valve is an electrically controlled valve.

8. The chromatography experimental method according to claim 6, characterized in that, The three-way valve is a manual valve.

9. The chromatography experimental method according to any one of claims 1-5, characterized in that, The number of inlet pipes is one.

Images