A method for determining the content of rhamnolipids

By combining HPLC-MS and HPLC-CAD, using a trifluoroacetic acid solution system and self-made standards, the specificity and sensitivity issues of rhamnolipid content detection were resolved, achieving efficient and accurate determination of multi-component rhamnolipid content.

CN117554521BActive Publication Date: 2026-06-12HUNAN LONGTENG BIOTECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HUNAN LONGTENG BIOTECH
Filing Date
2023-11-21
Publication Date
2026-06-12

AI Technical Summary

Technical Problem

Existing methods for detecting rhamnolipid content have poor specificity and low sensitivity, cannot be directly quantified, and are complex to operate, making it difficult to accurately detect multi-component mixtures.

Method used

HPLC-MS and HPLC-CAD methods were used, with trifluoroacetic acid aqueous solution and trifluoroacetic acid-containing acetonitrile solution as mobile phases for gradient elution. Combined with self-made rhamnolipin standards, the peak positions of each component were qualitatively analyzed by HPLC-MS, the peak areas were determined by HPLC-CAD, and the concentrations were calculated using the linear external standard method.

Benefits of technology

It improves the specificity, sensitivity and accuracy of detection, simplifies the pretreatment process, reduces operational complexity and improves the reproducibility of detection.

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Abstract

The present application relates to a kind of methods for determining rhamnolipid content, with rhamnolipid-containing solution as standard solution, HPLC-MS detection is carried out, mobile phase A is trifluoroacetic acid aqueous solution, mobile phase B is trifluoroacetic acid-containing acetonitrile solution, gradient elution is carried out, the position of each rhamnolipid component is determined;Different concentrations of standard solution are detected by HPLC-CAD, the relationship between the peak area of each rhamnolipid component and concentration is calculated, and the regression equation is obtained;Test sample is detected by HPLC-CAD, the peak area of each rhamnolipid component is calculated, and the rhamnolipid content in test sample is obtained by combining the regression equation;The present application does not need complex pretreatment process, is more time-saving and labor-saving, and has higher specificity, sensitivity and accuracy, and better reproducibility.
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