Application of linalool in prevention and treatment of anthracnose of sorghum

Abstract

This invention belongs to the field of plant disease and pest control technology, specifically disclosing the application of linalool in the control of sorghum anthracnose. In vitro antibacterial tests showed that linalool at concentrations of 0.2–1.2 mg / L significantly inhibited the growth of *Anthracnose fungus* on sorghum, with the diameter of the fungal masses being significantly lower than the control group. Seven days after inoculating different sorghum varieties with *Anthracnose fungus* treated with linalool, observations of the disease course and severity were conducted. Compared with the control, the use of 0.8 mg / L linalool significantly reduced the lesion area of ​​sorghum anthracnose. Therefore, linalool is a drug that conforms to green and ecological control practices and is of great significance in the prevention and control of sorghum anthracnose.

Description

Technical Field

[0001] This invention belongs to the field of plant disease and pest control technology, specifically relating to the application of linalool in the control of anthracnose in sorghum. Background Technology

[0002] Anthracnose in sorghum in my country is mainly caused by *Colletotrichum sublineola*, a fungus belonging to the genus *Colletotrichum*, whose conidiophores are black. Anthracnose is a significant disease of sorghum, occurring in all sorghum-producing areas. Currently, it is mainly controlled through chemical agents; however, long-term and excessive application of chemical agents can lead to drug resistance and pesticide residues.

[0003] Therefore, there is an urgent need to develop a safe and green antibacterial agent for the prevention and control of anthracnose disease caused by C. sublineola in sorghum. Summary of the Invention

[0004] The purpose of this invention is to provide new applications for linalool, specifically in the prevention and control of anthracnose in sorghum or in the inhibition of the growth of anthracnose fungus (Colletotrichum sublineola).

[0005] To achieve the above objectives, the present invention provides the following technical solution:

[0006] In vitro antibacterial tests showed that linalool at concentrations of 0.2–1.2 mg / L significantly inhibited the growth of *Bacillus anthracis* in sorghum, and the diameter of the bacterial masses was significantly smaller than that of the control group. The inhibitory effect of linalool at concentrations of 0.6–1.2 mg / L was the best.

[0007] Anthracnose bacteria were inoculated into different sorghum varieties treated with linalool for 7 days. The disease course and disease severity were then observed. The results showed that, compared with the control, the use of 0.8 mg / L linalool significantly reduced the lesion area of ​​sorghum anthracnose. Therefore, it can be used to control sorghum anthracnose. Attached Figure Description

[0008] Figure 1 The effects of different concentrations of linalool on the growth of *Colletotrichum sublineola* in sorghum. A. Growth status of *Colletotrichum sublineola* on culture media containing different concentrations of linalool (0, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2 mg / L); B. Statistical results of *Colletotrichum sublineola* plaque diameter under different concentrations of linalool; C. Changes in *Colletotrichum sublineola* plaque diameter after treatment with 0.8 mg / L linalool.

[0009] Figure 2This section shows the leaf lesion infection status of sorghum after linalool treatment. A. Leaf morphology of *Hypericum hongyingzi* and 115 after inoculation with *Hypericum hongyingzi*; B. Statistical results of lesion area of ​​*Hypericum hongyingzi* and 115 after inoculation with *Hypericum hongyingzi*. Mock is the water control, L is 0.8 mg / L linalool treatment, L+T is inoculation with *Hypericum hongyingzi* and linalool treatment, and T is inoculation with *Hypericum hongyingzi* only. *Hypericum hongyingzi* and 115 are different sorghum varieties. Detailed Implementation

[0010] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0011] Example 1: Inhibitory effect of linalool on the growth of *Anthracis chinensis* in sorghum.

[0012] To identify the inhibitory effect of linalool on *Colletotrichum sublineola*, 5 mm diameter *Colletotrichum sublineola* mycelial blocks were punched and added to PDA solid medium containing different concentrations of linalool (0, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2 mg / L). After 7 days of growth, the diameter of the mycelial blocks was observed. Statistical analysis showed that all concentrations of linalool had a significant inhibitory effect on *Colletotrichum sublineola*, with the mycelial block diameter significantly lower than the control group. The 0.6–1.2 mg / L linalool concentration showed the best inhibitory effect on *Colletotrichum sublineola*. Figure 1 ).

[0013] Example 2: The effect of linalool on the prevention and control of anthracnose in sorghum.

[0014] Sorghum plants (two varieties, Hongyingzi and 115) were divided into four groups: control plants (without anthracnose inoculation), linalool (0.8 mg / L) treatment group, anthracnose treatment group, and anthracnose treatment + linalool (0.8 mg / L) treatment group. The anthracnose inoculation concentration was 10. 6 CFU / ml, with a spraying volume of 20 μL per inoculated leaf. Disease progression and severity were observed 7 days after inoculation of different sorghum varieties with anthracnose. Statistical analysis revealed that, compared to the control, the use of 0.8 mg / L linalool significantly reduced the area of ​​anthracnose lesions. Figure 2 ).

[0015] As demonstrated by the above examples, linalool has a good control effect on anthracnose in sorghum. Compared with existing anthracnose inhibitors on the market, linalool is a plant-derived volatile compound, leaving less residue and showing significant efficacy in the control of anthracnose in sorghum.

Claims

1. Linalool in the prevention and treatment of anthrax caused by bacteria Colletotrichum sublineola Its application in sorghum anthracnose is characterized by, The concentration of linalool used is 0.6 ~ 1.0 mg / L.

2. Linalool inhibits anthrax bacteria. Colletotrichum sublineola Applications during growth, characterized by, The concentration of linalool used is 0.6 ~ 1.0 mg / L.

3. The application according to claim 2, characterized in that, The concentration of linalool used is 0.8 mg / L.

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