A method for improving the ability of kiwifruit to resist soft rot

Abstract

The application discloses a method for improving the soft rot resistance of kiwifruit, and specifically relates to a method for irradiating the picked kiwifruit with blue light. 2 The wavelength of the blue light is 450 nm, and the light intensity of the blue light is 75 umol / m 2 / s or more. The method can effectively improve the soft rot resistance of the kiwifruit, reduce the incidence of soft rot of the kiwifruit during storage, prolong the storage time of the kiwifruit, and is simple in operation and convenient for large-scale use, thereby effectively solving the problems in the prior art.

Description

Technical Field

[0001] This invention belongs to the field of kiwifruit preservation technology, specifically relating to a method for improving the resistance of kiwifruit to soft rot. Background Technology

[0002] In recent years, with the continuous development of the kiwifruit industry and the expansion of its planting area, the problem of diseases has become increasingly prominent. The rapid spread and difficulty in controlling kiwifruit diseases have become one of the important factors restricting the development of the kiwifruit industry. Recent studies have shown that there are more than 30 common diseases of kiwifruit, including brown spot, flower rot, fruit soft rot, root-knot nematode disease, root rot, black spot, and bacterial canker. Among them, kiwifruit soft rot is extremely serious during the post-harvest storage period, with a general incidence rate of about 20%, and as high as 50% in severe cases, greatly hindering the development of the kiwifruit industry.

[0003] Currently, the main method for controlling soft rot is still chemical spraying of pesticides, which not only causes great harm to the environment but also leaves pesticide residues. Although there is some research on biological control of soft rot, its application in actual production is still very limited, which is not conducive to large-scale promotion. Summary of the Invention

[0004] To address the aforementioned shortcomings in the existing technology, this invention provides a method for improving the resistance of kiwifruit to soft rot. This method can effectively enhance the resistance of kiwifruit to soft rot, reduce the incidence of soft rot during storage, and extend the storage time of kiwifruit. At the same time, this method is simple to operate and easy to use on a large scale, effectively solving the problems existing in the prior art.

[0005] To achieve the above objectives, the technical solution adopted by the present invention to solve its technical problem is as follows:

[0006] One method to improve the resistance of kiwifruit to soft rot is to irradiate the harvested kiwifruit with blue light.

[0007] Furthermore, the wavelength of blue light is 450-500nm.

[0008] Furthermore, the blue light irradiation intensity was 75 μmol / m 2 / s or more.

[0009] Furthermore, the irradiation treatment time is more than 16 hours per day.

[0010] The beneficial effects of this invention are as follows:

[0011] 1. In this invention, blue light with a wavelength of 450nm is used to treat harvested kiwifruit. Blue light can increase the relative expression levels of redox-related genes (SOD, CAT, POD) and disease resistance genes (PR1), thereby improving the kiwifruit's resistance to soft rot. At the same time, blue light irradiation can also increase the ascorbic acid content in kiwifruit, improving the fruit's resistance to adverse conditions. Finally, blue light can also inhibit the growth of Staphylococcus aureus, the main pathogen of soft rot, reducing its impact on kiwifruit.

[0012] 2. The method in this invention only requires irradiation with blue light, which has the advantages of being simple, convenient and low cost. It does not require the use of chemical reagents and will not have any impact on the environment or human body. It also does not require the use of biological methods, which can greatly improve the efficiency of operation.

[0013] 3. The method in this invention can be used to treat kiwifruit by irradiation during the sale of kiwifruit, thereby extending the shelf life of kiwifruit. Attached Figure Description

[0014] Figure 1 The natural disease development of hardy kiwifruit after exposure to different light sources for different durations;

[0015] Figure 2 The natural disease incidence rate of hardy kiwifruit after exposure to different light sources for different durations;

[0016] Figure 3 The changes in the diameter of disease spots on Hongyang kiwifruit after exposure to different light sources for different durations;

[0017] Figure 4 A statistical chart showing the diameter of disease spots on Hongyang kiwifruit after exposure to different light sources for different durations.

[0018] Figure 5 Line graph showing the change in diameter of diseased spots on Hongyang kiwifruit after exposure to different light sources for different durations;

[0019] Figure 6 The changes in the diameter of diseased spots on Xinzhong No. 1 kiwifruit after exposure to different light sources for different durations;

[0020] Figure 7 A statistical chart showing the diameter of disease spots on Xinzhong No. 1 kiwifruit after exposure to different light sources for different durations.

[0021] Figure 8 Line graph showing the change in diameter of diseased spots on Xinzhong No. 1 kiwifruit after exposure to different light sources for different durations;

[0022] Figure 9 The growth of mycelium in the culture medium after different light treatments;

[0023] Figure 10 A statistical graph showing the diameter of mycelial growth in culture medium after different light treatments;

[0024] Figure 11 Line graph showing the change in hyphal diameter in culture medium after different light treatments;

[0025] Figure 12 The growth of mycelia in the culture medium after 10 days of continuous irradiation. Detailed Implementation

[0026] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to embodiments. It should be understood that the specific embodiments described herein are only for explaining the invention and are not intended to limit the invention; that is, the described embodiments are merely some embodiments of the invention, and not all embodiments.

[0027] Therefore, the following detailed description of the embodiments of the present invention is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.

[0028] It should be noted that relational terms such as "first" and "second" are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus. Unless otherwise specified, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes the element.

[0029] The features and performance of the present invention will be further described in detail below with reference to the embodiments and accompanying drawings.

[0030] Example 1

[0031] One method to improve the resistance of kiwifruit to soft rot involves irradiating the harvested kiwifruit with blue light; the wavelength of the blue light is 450 nm, and the irradiation intensity is 75 μmol / m. 2 / s, irradiation treatment time is 16h / day.

[0032] Example 2

[0033] One method to improve the resistance of kiwifruit to soft rot involves irradiating the harvested kiwifruit with blue light; the wavelength of the blue light is 500 nm, and the irradiation intensity is 85 μmol / m. 2 / s, irradiation treatment time is 18h / day.

[0034] Example 3

[0035] One method to improve the resistance of kiwifruit to soft rot involves irradiating the harvested kiwifruit with blue light; the wavelength of the blue light is 480nm, and the irradiation intensity is 95umol / m. 2 / s, irradiation treatment time is 20h / day.

[0036] Experimental Example

[0037] 1. Observe the natural disease development of kiwifruit under different light conditions;

[0038] Freshly picked hardy kiwifruit were thoroughly washed with running water, dried, and divided into five groups. Each group was treated with different light sources: dark treatment, white light treatment, blue light (450nm) treatment, green light (520nm) treatment, and red light (660nm) treatment. The specific irradiation methods followed those in Example 1. The natural disease development of the hardy kiwifruit was observed over time. Specific results are shown in [link to example]. Figure 1-2 .

[0039] Figure 1 The natural disease development of hardy kiwifruit after exposure to different light sources for different durations;

[0040] Figure 2 The natural disease incidence rate of hardy kiwifruit after exposure to different light sources for different durations;

[0041] pass Figure 1-2 It can be seen that, under natural conditions, the kiwifruit treated with darkness, white light, green light, and red light began to show symptoms on the fourth day, while the kiwifruit treated with blue light did not show symptoms at this time. As time progressed, the incidence of disease in the kiwifruit treated with darkness, white light, green light, and red light gradually increased, while the incidence of disease in the kiwifruit treated with blue light was the lowest. This preliminarily proves that blue light irradiation can inhibit the occurrence of soft rot in kiwifruit. II. Observation of disease incidence in kiwifruit after inoculation with pathogens under different light conditions;

[0042] Freshly picked Hongyang kiwifruit were thoroughly washed with running water, air-dried, disinfected with 75% alcohol, and then air-dried in the shade before being divided into five groups. Wounds (0.5 cm deep) were created on each group by puncturing the fruit with a sterile inoculation needle. Using a 0.5 cm diameter sterile punch, identical mycelial cakes were created from the edge of a pre-cultured *Botrytis cinerea* agar plate. The mycelial side of the mycelial cake was then placed against the wound on the kiwifruit. The cakes were then placed on moist filter paper and kept at 25°C. Each group of kiwifruit was then subjected to dark treatment, white light treatment, blue light treatment, green light treatment, and red light treatment, respectively, following the specific irradiation methods described in Example 1. Disease incidence was observed over time. Specific results are shown in [link to example]. Figure 3-5 .

[0043] Figure 3 The changes in the diameter of disease spots on Hongyang kiwifruit after exposure to different light sources for different durations;

[0044] Figure 4 A statistical chart showing the diameter of disease spots on Hongyang kiwifruit after exposure to different light sources for different durations.

[0045] Figure 5 Line graph showing the change in diameter of diseased spots on Hongyang kiwifruit after exposure to different light sources for different durations;

[0046] pass Figure 3-5 It can be seen that after inoculating *Botrytis cinerea* into Hongyang kiwifruit, starting from day 5, the diameter of the bacterial patches treated with blue light was significantly smaller than that of the bacterial patches treated with other light, further proving that blue light irradiation has a certain inhibitory effect on the severity of soft rot in Hongyang kiwifruit.

[0047] The same experiment was conducted on Xinzhong No. 1 kiwifruit using the above method. Specific experimental results are shown below. Figure 6-8 .

[0048] Figure 6 The changes in the diameter of diseased spots on Xinzhong No. 1 kiwifruit after exposure to different light sources for different durations;

[0049] Figure 7 A statistical chart showing the diameter of disease spots on Xinzhong No. 1 kiwifruit after exposure to different light sources for different durations.

[0050] Figure 8 Line graph showing the change in diameter of diseased spots on Xinzhong No. 1 kiwifruit after exposure to different light sources for different durations;

[0051] pass Figure 6-8 This also demonstrates that blue light irradiation treatment has a certain inhibitory effect on the incidence of soft rot in Xinzhong No. 1 kiwifruit.

[0052] 3. Equal amounts of *Botrytis cinerea* were inoculated into PDA medium, and the medium was then placed under different light treatment conditions, following the irradiation method described in Example 1. Changes in hyphal diameter were observed and recorded on days 3 and 4. Specific results are shown in [link to example]. Figure 9-11 After 10 consecutive days of treatment, continue to observe the changes in mycelium, see details below. Figure 12 .

[0053] Figure 9 The growth of mycelium in the culture medium after different light treatments;

[0054] Figure 10 A statistical graph showing the diameter of mycelial growth in culture medium after different light treatments;

[0055] Figure 11 Line graph showing the change in hyphal diameter in culture medium after different light treatments;

[0056] Figure 12 The growth of mycelia in the culture medium after 10 days of continuous irradiation;

[0057] pass Figure 9-11 It can be seen that the mycelial diameter after blue light irradiation is significantly smaller than that of other treatment groups.

[0058] pass Figure 12 It can be seen that after 10 days of continuous culture, the culture medium irradiated with blue light had the lowest mycelial content, which further proves that blue light irradiation can inhibit mycelial growth.

Claims

1. Application of blue light irradiation in improving the resistance of kiwifruit to soft rot, wherein the improvement of the resistance to soft rot is achieved by inhibiting the growth of *Botrytis cinerea*, and the intensity of the blue light irradiation is 75 μmol / m². 2 / s or more; the irradiation treatment time is 16h / day or more; the wavelength of blue light is 450-500nm.

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