Production method of water-retaining agent for aquatic products

Abstract

The invention relates to the technical field of water-retaining agent production, and in particular to a production method of a water-retaining agent for aquatic products. EDTA nanoparticles are blended with a PVP solution in an electrostatic environment so that the EDTA nanoparticles are adsorbed onto PVP molecular chains. EDTA can chelate magnesium ions and calcium ions in muscle protein to loosen the protein structure. Hydroxyproline is reacted with the PVP solution so that the water-retaining agent has better biocompatibility with aquatic products, can improve the relaxation of muscle proteins in aquatic products, and can form a more uniform and delicate three-dimensional network structure of PVP. Papain is combined with PVP to promote the dissociation of motor globulin in aquatic products into actin and myosin in a saline solution. The prepared water-retaining agent for aquatic products can achieve a water-retaining effect equivalent to that of a composite phosphate water-retaining agent.

Description

Technical Field

[0001] The present invention relates to the technical field of water-retaining agent production, in particular to a production method of a water-retaining agent for aquatic products. Background Art

[0002] Water retention is a type of substance used to maintain the water retention of food during food processing and improve the shape, flavor and color of food. The existing water retention agents used in food are phosphates. Although phosphates themselves have good water retention properties, excessive intake of phosphates by the human body is not conducive to the calcium-phosphorus balance of the human body.

[0003] At present, most water-retaining agents used in aquatic products still use composite phosphates. Therefore, there is an urgent need to develop a production method for a phosphate-free water-retaining agent for aquatic products so that the prepared water-retaining agent for aquatic products can achieve a water-retaining effect equivalent to that of a composite phosphate water-retaining agent. Summary of the Invention

[0004] The technical problem to be solved by the present invention is: there is an urgent need to develop a production method for a phosphorus-free water-retaining agent for aquatic products, so that the prepared water-retaining agent for aquatic products can achieve a water-retaining effect equivalent to that of a composite phosphate water-retaining agent.

[0005] In order to solve the above technical problems, the technical solution adopted by the present invention is:

[0006] A method for producing a water-retaining agent for aquatic products, comprising the following steps:

[0007] Step 1: Mix 2.5-3.5 parts by mass of PVP powder, 3-6 parts by mass of sodium carbonate, 3-5 parts by mass of sodium chloride and 90-100 parts by mass of deionized water into a reactor, stir evenly, and prepare a PVP solution;

[0008] Step 2: Add 0.5-1 parts by mass of hydroxyproline to the reaction kettle, stir evenly at 75-80°C, and react for 10-15 minutes;

[0009] Step 3: Lower the temperature in the reactor to 50-60°C, add 0.8-1 parts by mass of EDTA into the reactor, generate static electricity in the reactor through an electrostatic generator, and react for 15-20 minutes under the action of static electricity;

[0010] Step 4: Lower the temperature in the reactor to 35-45°C, add 0.08-0.14 parts by weight of papain into the reactor, and react for 15-20 minutes under the action of electrostatics;

[0011] Step 5: Add 15-20 parts by weight of trehalose into the reactor and fully dissolve it;

[0012] Step 6: freeze-dry the solution in the reactor and grind it to obtain a water-retaining agent for aquatic products.

[0013] Furthermore, in the above-mentioned method for producing a water-retaining agent for aquatic products, after step 5, the method further comprises step 51: adding sodium hydroxide solution into the reaction kettle to adjust the pH value to 9-10.

[0014] Furthermore, in the above-mentioned method for producing aquatic product moisture-retaining agent, step 5 further comprises adding 2-4 parts by mass of ice structure protein into the reaction kettle.

[0015] Furthermore, in the above-mentioned production method of the water-retaining agent for aquatic products, step 1 is specifically: 3 parts by mass of PVP powder, 4 parts by mass of sodium carbonate, 4 parts by mass of sodium chloride and 95 parts by mass of deionized water are mixed and added into a reactor, stirred evenly, and prepared into a PVP solution.

[0016] Furthermore, in the above-mentioned method for producing the water-retaining agent for aquatic products, the step 2 is specifically as follows: adding 0.7 parts by mass of hydroxyproline into a reaction kettle, stirring evenly at 78° C., and reacting for 12 minutes.

[0017] Furthermore, in the above-mentioned production method of the water-retaining agent for aquatic products, step 3 specifically comprises: lowering the temperature in the reactor to 55° C., adding 0.9 parts by mass of EDTA into the reactor, generating static electricity in the reactor through an electrostatic generator, and reacting for 17 minutes under the action of static electricity.

[0018] Furthermore, in the above-mentioned method for producing a water-retaining agent for aquatic products, the step 5 specifically comprises: adding 17 parts by mass of trehalose into a reaction kettle and fully dissolving it.

[0019] The present invention also relates to a water-retaining agent for aquatic products prepared by the production method of the water-retaining agent for aquatic products.

[0020] The beneficial effects of the present invention are as follows: PVP itself is a porous polymer material. By blending EDTA nanoparticles with PVP solution under an electrostatic environment, the EDTA nanoparticles are adsorbed on the PVP molecular chain. EDTA can chelate magnesium ions and calcium ions in muscle protein, release the carboxyl groups of muscle protein in aquatic products, make the protein negatively charged, thereby increasing the electrostatic repulsion between hydroxyl groups, resulting in a loose protein structure. By reacting hydroxyproline with the PVP solution, the hydroxyproline is hydrogen-bonded with the amino groups on the PVP molecular chain through the hydroxyl group, so that the water retaining agent has the same properties as aquatic products. The products have better biocompatibility, can improve the relaxation between muscle proteins in aquatic products, and can also make PVP form a more uniform and delicate three-dimensional network structure, thereby improving water retention performance. Through the binding reaction of papain and PVP, the papain is physically adsorbed on the molecular chain of PVP through electrostatic action. Papain can promote the dissociation of motor globulin in aquatic products into actin and myosin in salt solution, thereby increasing the water retention of aquatic products and improving the tenderness of aquatic products. The prepared aquatic product water retention agent can achieve a water retention effect equivalent to that of the composite phosphate water retention agent. DETAILED DESCRIPTION

[0021] To explain the technical content, achieved objectives and effects of the present invention in detail, the following describes them in conjunction with the implementation methods.

[0022] The specific embodiment of the present invention relates to a method for producing a water-retaining agent for aquatic products, comprising the following steps:

[0023] Step 1: Mix 2.5-3.5 parts by mass of PVP powder, 3-6 parts by mass of sodium carbonate, 3-5 parts by mass of sodium chloride and 90-100 parts by mass of deionized water into a reactor, stir evenly, and prepare a PVP solution;

[0024] Step 2: Add 0.5-1 parts by mass of hydroxyproline to the reaction kettle, stir evenly at 75-80°C, and react for 10-15 minutes;

[0025] Step 3: Lower the temperature in the reactor to 50-60°C, add 0.8-1 parts by mass of EDTA into the reactor, generate static electricity in the reactor through an electrostatic generator, and react for 15-20 minutes under the action of static electricity;

[0026] Step 4: Lower the temperature in the reactor to 35-45°C, add 0.08-0.14 parts by weight of papain into the reactor, and react for 15-20 minutes under the action of electrostatics;

[0027] Step 5: Add 15-20 parts by weight of trehalose into the reactor and fully dissolve it;

[0028] Step 6: freeze-dry the solution in the reactor and grind it to obtain a water-retaining agent for aquatic products.

[0029] In the above embodiment, PVP itself is a porous polymer material. By blending EDTA nanoparticles with PVP solution under an electrostatic environment, the EDTA nanoparticles are adsorbed on the PVP molecular chain. EDTA can chelate magnesium ions and calcium ions in muscle protein, release the carboxyl groups of muscle protein in aquatic products, and make the protein negatively charged, thereby increasing the electrostatic repulsion between hydroxyl groups, resulting in a loose protein structure. By reacting hydroxyproline with PVP solution, hydroxyproline is hydrogen bonded to the amino groups on the PVP molecular chain through the hydroxyl group, so that the water retention agent has the same properties as aquatic products. It has better biocompatibility between products, can improve the relaxation between muscle proteins in aquatic products, and can make PVP form a more uniform and delicate three-dimensional network structure, thereby improving water retention performance. Through the binding reaction of papain and PVP, the papain is physically adsorbed on the molecular chain of PVP through electrostatic action. Papain can promote the dissociation of motor globulin in aquatic products into actin and myosin in salt solution, thereby increasing the water retention of aquatic products and improving the tenderness of aquatic products. The prepared aquatic product water retention agent can achieve a water retention effect equivalent to that of the composite phosphate water retention agent.

[0030] As a preferred embodiment, after step 5, the process further includes step 51: adding sodium hydroxide solution into the reaction kettle to adjust the pH value to 9-10.

[0031] As the pH value of the system rises, when the moisture retainer is used, the calcium in the aquatic product activates the papain to tenderize the aquatic meat product.

[0032] As a preferred embodiment, the step 5 further comprises adding 2-4 parts by mass of ice structure protein into the reaction kettle.

[0033] When acting on aquatic products, ice-structuring proteins can prevent the formation of ice crystals and reduce water loss and dehydration during the thawing process of aquatic products.

[0034] As a preferred embodiment, the step 1 is specifically as follows: 3 parts by mass of PVP powder, 4 parts by mass of sodium carbonate, 4 parts by mass of sodium chloride and 95 parts by mass of deionized water are mixed and added into a reactor, and stirred evenly to prepare a PVP solution.

[0035] As a preferred embodiment, the step 2 specifically comprises: adding 0.7 parts by mass of hydroxyproline into a reaction kettle, stirring evenly at 78° C., and reacting for 12 minutes.

[0036] As a preferred embodiment, the step 3 specifically comprises: lowering the temperature in the reactor to 55° C., adding 0.9 parts by mass of EDTA into the reactor, generating static electricity in the reactor through an electrostatic generator, and reacting for 17 minutes under the action of static electricity.

[0037] As a preferred embodiment, the step 5 specifically includes: adding 17 parts by mass of trehalose into the reactor and fully dissolving it.

[0038] Example 1

[0039] A method for producing a water-retaining agent for aquatic products, comprising the following steps:

[0040] Step 1: 3 parts by mass of PVP powder, 4.5 parts by mass of sodium carbonate, 4 parts by mass of sodium chloride and 95 parts by mass of deionized water were mixed and added to a reactor, stirred evenly to prepare a PVP solution;

[0041] Step 2: Add 0.7 parts by mass of hydroxyproline to the reaction kettle, stir evenly at 78°C, and react for 12 minutes;

[0042] Step 3: Lower the temperature in the reactor to 55°C, add 0.9 parts by mass of EDTA into the reactor, generate static electricity in the reactor using an electrostatic generator, and react for 17 minutes under the action of static electricity;

[0043] Step 4: Lower the temperature in the reactor to 40°C, add 0.08-0.14 parts by weight of papain into the reactor, and react for 17 minutes under the action of electrostatics;

[0044] Step 5: Add 17 parts by mass of trehalose and 3 parts by mass of ice structure protein to the reactor and fully dissolve them;

[0045] Step 51: adding sodium hydroxide solution to the reaction kettle and adjusting the pH value to 9.5;

[0046] Step 6: freeze-dry the solution in the reactor and grind it to obtain a water-retaining agent for aquatic products.

[0047] Example 2

[0048] A method for producing a water-retaining agent for aquatic products, comprising the following steps:

[0049] Step 1: 2.5 parts by mass of PVP powder, 3 parts by mass of sodium carbonate, 3 parts by mass of sodium chloride and 90-100 parts by mass of deionized water were mixed and added to a reactor, stirred evenly to prepare a PVP solution;

[0050] Step 2: Add 0.5 parts by mass of hydroxyproline to the reaction kettle, stir evenly at 75°C, and react for 10 minutes;

[0051] Step 3: Lower the temperature in the reactor to 50°C, add 0.8 parts by mass of EDTA into the reactor, generate static electricity in the reactor using an electrostatic generator, and react for 15-20 minutes under the action of static electricity;

[0052] Step 4: Lower the temperature in the reactor to 35°C, add 0.08 parts by mass of papain into the reactor, and react for 15 minutes under the action of electrostatics;

[0053] Step 5: Add 15 parts by mass of trehalose and 2 parts by mass of ice structure protein to the reactor and fully dissolve them;

[0054] Step 51: Add sodium hydroxide solution to the reaction kettle and adjust the pH value to 9;

[0055] Step 6: freeze-dry the solution in the reactor and grind it to obtain a water-retaining agent for aquatic products.

[0056] Example 3

[0057] A method for producing a water-retaining agent for aquatic products, comprising the following steps:

[0058] Step 1: 3.5 parts by mass of PVP powder, 6 parts by mass of sodium carbonate, 5 parts by mass of sodium chloride and 100 parts by mass of deionized water were added to a reactor and stirred to prepare a PVP solution;

[0059] Step 2: Add 1 part by mass of hydroxyproline to the reaction kettle, stir evenly at 80°C, and react for 15 minutes;

[0060] Step 3: Lower the temperature in the reactor to 60°C, add 1 part by mass of EDTA into the reactor, generate static electricity in the reactor using an electrostatic generator, and react for 20 minutes under the action of static electricity;

[0061] Step 4: Lower the temperature in the reactor to 45°C, add 0.14 parts by mass of papain into the reactor, and react for 20 minutes under the action of electrostatics;

[0062] Step 5: Add 20 parts by mass of trehalose and 4 parts by mass of ice structure protein to the reactor and fully dissolve them;

[0063] Step 51: Add sodium hydroxide solution to the reaction kettle and adjust the pH value to 10;

[0064] Step 6: freeze-dry the solution in the reactor and grind it to obtain a water-retaining agent for aquatic products.

[0065] Comparative Example 1

[0066] The method for producing the water-retaining agent for aquatic products described in Example 1 is different in that step 2 is omitted.

[0067] Comparative Example 2

[0068] The method for producing the water-retaining agent for aquatic products described in Example 1 is different in that step 3 is omitted.

[0069] Comparative Example 3

[0070] The method for producing the water-retaining agent for aquatic products described in Example 1 is different in that step 4 is omitted.

[0071] Comparative Example 4

[0072] The method for producing the water-retaining agent for aquatic products described in Example 1 is different in that step 51 is omitted.

[0073] Comparative Example 5

[0074] A moisture retaining agent comprises phosphate, wherein the phosphate is a mixture of sodium tripolyphosphate, sodium pyrophosphate and sodium hexametaphosphate in a mass ratio of 2:2:1.

[0075] Performance test of water retention agent:

[0076] Experimental method: Prepare a piece of fish meat with basically the same tissue structure, cut it into 6 pieces of equal weight, and perform the following experiments on each piece;

[0077] Experimental Example 1

[0078] The moisture retaining agent prepared in Example 1 was evenly applied on the surface of one of the fish fillets, and the amount of moisture retaining agent added was 0.6% of the total weight of the fish fillet.

[0079] Experimental Example 2

[0080] The moisture retaining agent prepared in Comparative Example 1 was evenly applied on the surface of one of the fish slices, and the amount of the moisture retaining agent added was 0.6% of the total weight of the fish slice.

[0081] Experimental Example 3

[0082] The moisture retaining agent prepared in Comparative Example 2 was evenly applied on the surface of one of the fish slices, and the amount of the moisture retaining agent added was 0.6% of the total weight of the fish slice.

[0083] Experimental Example 4

[0084] The moisture retaining agent prepared in Comparative Example 3 was evenly applied on the surface of one of the fish slices, and the amount of the moisture retaining agent added was 0.6% of the total weight of the fish slice.

[0085] Experimental Example 5

[0086] The moisture retaining agent prepared in Comparative Example 4 was evenly applied on the surface of one of the fish slices, and the amount of the moisture retaining agent added was 0.6% of the total weight of the fish slice.

[0087] Experimental Example 6

[0088] The moisture retaining agent prepared in Comparative Example 5 was evenly applied on the surface of one of the fish slices, and the amount of the moisture retaining agent added was 0.6% of the total weight of the fish slice.

[0089] The six fish fillets processed in Experimental Examples 1-6 were frozen at -20°C for 6 hours, taken out, and thawed at room temperature for 6 hours. The thawing weight loss rate of each fish fillet was calculated. The five thawed fish fillets were then steamed in the same boiling water pot for 5 minutes, and the cooking loss rate of each fish fillet was calculated.

[0090] Wherein, thawing weight loss rate = original weight of fish fillet before freezing - weight after thawing / original weight × 100%;

[0091] Wherein, cooking loss rate = weight after thawing - weight after cooking / weight after thawing × 100%;

[0092] The experimental results are shown in Table 1:

[0093] Table 1

[0094]

[0095] As can be seen from the results in Table 1, the water retention effect of the fish fillet in Experimental Example 6 is the best, followed by Experimental Example 1. However, the results of Experimental Examples 1 and 6 are very similar, indicating that the water retention agent for aquatic products prepared in Example 1 can basically achieve a water retention effect equivalent to that of the composite phosphate water retention agent.

[0096] The above descriptions are merely embodiments of the present invention and are not intended to limit the patent scope of the present invention. Any equivalent transformations made using the contents of the present invention specification, or directly or indirectly applied in related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A method for producing a water-retaining agent for aquatic products, characterized in that: The following steps are involved: Step 1: Mix 2.5-3.5 parts by mass of PVP powder, 3-6 parts by mass of sodium carbonate, 3-5 parts by mass of sodium chloride and 90-100 parts by mass of deionized water into a reactor, stir evenly, and prepare a PVP solution; Step 2: Add 0.5-1 parts by mass of hydroxyproline to the reaction kettle, stir evenly at 75-80°C, and react for 10-15 minutes; Step 3: Lower the temperature in the reactor to 50-60°C, add 0.8-1 parts by mass of EDTA into the reactor, generate static electricity in the reactor through an electrostatic generator, and react for 15-20 minutes under the action of static electricity; Step 4: Lower the temperature in the reactor to 35-45°C, add 0.08-0.14 parts by weight of papain into the reactor, and react for 15-20 minutes under the action of electrostatics; Step 5: Add 15-20 parts by weight of trehalose into the reactor and fully dissolve it; Step 6: freeze-dry the solution in the reactor and grind it to obtain a water-retaining agent for aquatic products.

2. The method for producing a water-retaining agent for aquatic products according to claim 1, characterized in that: After step 5, the method further includes step 51: adding sodium hydroxide solution into the reaction kettle to adjust the pH value to 9-10.

3. The method for producing a water-retaining agent for aquatic products according to claim 1, characterized in that: The step 5 further includes adding 2-4 parts by mass of ice structure protein into the reaction kettle.

4. The method for producing a water-retaining agent for aquatic products according to claim 1, characterized in that: The step 1 is specifically as follows: 3 parts by mass of PVP powder, 4 parts by mass of sodium carbonate, 4 parts by mass of sodium chloride and 95 parts by mass of deionized water are mixed and added into a reactor, and stirred evenly to prepare a PVP solution.

5. The method for producing a water-retaining agent for aquatic products according to claim 1, characterized in that: The step 2 specifically comprises: adding 0.7 parts by mass of hydroxyproline into a reaction kettle, stirring evenly at 78° C., and reacting for 12 minutes.

6. The method for producing a water-retaining agent for aquatic products according to claim 1, characterized in that: The step 3 specifically comprises: lowering the temperature in the reactor to 55° C., adding 0.9 parts by mass of EDTA into the reactor, generating static electricity in the reactor through an electrostatic generator, and reacting for 17 minutes under the action of static electricity.

7. The method for producing a water-retaining agent for aquatic products according to claim 1, characterized in that: The step 5 specifically includes: adding 17 parts by mass of trehalose into the reactor and fully dissolving it.

8. The water-retaining agent for aquatic products produced by the production method of the water-retaining agent for aquatic products according to any one of claims 1 to 7.

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