A urinary catheter

Abstract

The application belongs to the field of medical treatment and health, and particularly relates to a urinary catheter. The urinary catheter of the embodiment of the application is entirely covered with a nano coating prepared from an aqueous solution of aminated multi-walled carbon nanotubes. The nano coating can prevent bacterial adhesion, thereby effectively reducing the occurrence of complications such as infection and thrombus during use of the urinary catheter, can reduce the friction coefficient of the surface of the urinary catheter, improve the smoothness and wear resistance of the surface, reduce damage to the urethra, and has long-term performance stability and biocompatibility, thereby avoiding potential toxicity and side effects.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of medical health, and particularly relates to a urinary catheter. BACKGROUND

[0002] Urinary dysfunction is a common condition with a certain incidence in people of different ages. Common causes include prostatic hyperplasia, overactive bladder, urethral stricture, neurogenic bladder, urinary tract infection, etc. When severe urinary dysfunction such as urinary retention occurs, urinary catheterization is one of the most commonly used methods.

[0003] Urinary catheterization can relieve the pressure in the bladder by directly inserting a urinary catheter into the urethra to drain urine out of the body, thereby alleviating symptoms. However, when using a urinary catheter, bacteria can easily enter the urethra, increasing the risk of urinary tract infection. Such infection can cause diseases such as cystitis, pyelonephritis, and sepsis; at the same time, the urinary catheter can cause discomfort and pain when inserted and removed.

[0004] Therefore, how to reduce infection and discomfort and pain caused by the use of a urinary catheter has attracted more and more attention. SUMMARY

[0005] The present application is based on the discovery and understanding of the inventors of the following facts and problems:

[0006] In order to reduce infection and reduce damage to the urethra when using a urinary catheter, a silver ion nano coating is coated on the surface of the urinary catheter in the prior art, which can reduce the risk of infection during urinary catheterization. However, silver ions are gradually released during use, resulting in a decrease in antibacterial effect and accumulation of silver ions in the body, which can cause potential toxicity and side effects, and silver ions can be irritating and toxic to some artificial materials.

[0007] The present application aims to at least partially solve one of the technical problems in the related art. To this end, an embodiment of the present application proposes a urinary catheter, which is entirely covered with a nano coating that can prevent bacterial adhesion, thereby effectively reducing the incidence of complications such as infection and thrombosis during urinary catheterization, while reducing the friction coefficient of the surface of the urinary catheter, improving the smoothness and wear resistance of the surface, reducing damage to the urethra, and having long-term performance stability and biocompatibility, thereby avoiding potential toxicity and side effects.

[0008] A urinary catheter according to an embodiment of the present application, wherein the urinary catheter is entirely covered with a nano coating.

[0009] The nano coating is prepared from an aqueous solution of aminated multi-walled carbon nanotubes.

[0010] The urethral catheter of the embodiment of the present application has the following advantages and technical effects: 1. The amino-functionalized multi-walled carbon nanotubes have high hydrophilicity, can adsorb and destroy microbial cell walls, and can effectively reduce frictional damage to the urethra; 2. The amino-functionalized multi-walled carbon nanotubes can provide long-term performance stability, so that the urethral catheter coated with the nano coating has a longer service life; 3. The urethral catheter modified by the nano coating containing the amino-functionalized multi-walled carbon nanotubes has higher biocompatibility, and can reduce the occurrence of irritation and rejection reactions; 4. The urethral catheter modified by the amino-functionalized multi-walled carbon nanotubes is not dependent on the release of the material in terms of antibacterial effect, and potential toxicity and side effects are avoided.

[0011] In some embodiments, the preparation method of the nano coating comprises the following steps:

[0012] (1) After the urethral catheter is soaked in an organic solvent, the urethral catheter is washed with water;

[0013] (2) The urethral catheter treated in the step (1) is soaked in an aqueous solution of amino-functionalized multi-walled carbon nanotubes, and is subjected to shaking treatment in a horizontal shaking table, and is naturally dried after being taken out.

[0014] In some embodiments, in the step (1), the organic solvent comprises chloroform.

[0015] In some embodiments, in the step (1), the soaking treatment time is 3-4 min.

[0016] In some embodiments, in the step (2), the concentration of the aqueous solution of amino-functionalized multi-walled carbon nanotubes is 0.1-0.5 mg / mL.

[0017] In some embodiments, in the step (2), the shaking treatment time is 5-6 h.

[0018] In some embodiments, in the step (2), the frequency of shaking in the horizontal shaking table is 80-100 rpm.

[0019] In some embodiments, in the step (2), before the natural drying, the urethral catheter is first washed with pure water.

[0020] In some embodiments, the thickness of the nano coating is 5-10 μm. BRIEF DESCRIPTION OF DRAWINGS

[0021] Figure 1 is a photo of the Staphylococcus aureus cultured and diluted 10 times by the aqueous solution of amino-functionalized multi-walled carbon nanotubes, wherein the right side is a photo magnified by 35 times;

[0022] Figure 2 is the photo of the Staphylococcus aureus after being cultured with the diluted 100 times aqueous solution of the amino-functionalized multi-walled carbon nanotubes, in which the right side is the photo after being enlarged 35 times;

[0023] Figure 3 is the photo of the Staphylococcus aureus after being cultured with the diluted 10 times aqueous solution of silver nitrate, in which the right side is the photo after being enlarged 35 times;

[0024] Figure 4 is the photo of the Staphylococcus aureus after being cultured with the diluted 100 times aqueous solution of silver nitrate, in which the right side is the photo after being enlarged 35 times;

[0025] Figure 5 is the photo of the Staphylococcus aureus after being cultured with the diluted 10 times in the control group, in which the right side is the photo after being enlarged 35 times;

[0026] Figure 6 is the photo of the Staphylococcus aureus after being cultured with the diluted 100 times in the control group, in which the right side is the photo after being enlarged 35 times. DETAILED DESCRIPTION

[0027] Embodiments of the present application are described in detail below with reference to examples shown in the accompanying drawings. The embodiments described below by reference to the drawings are exemplary and are intended to explain the present application, and cannot be understood as a limitation of the present application.

[0028] The urinary catheter of the embodiment of the present application is entirely covered with a nano coating;

[0029] The nano coating is prepared from an aqueous solution of amino-functionalized multi-walled carbon nanotubes.

[0030] The urinary catheter of the embodiment of the present application, the amino-functionalized multi-walled carbon nanotubes have high hydrophilicity, can adsorb and destroy microbial cell walls while effectively reducing frictional damage to the urethra; the amino-functionalized multi-walled carbon nanotubes can provide long-term performance stability, enabling the urinary catheter covered with the nano coating to have a longer service life; the urinary catheter modified with the nano coating containing the amino-functionalized multi-walled carbon nanotubes has higher biocompatibility, which can reduce the occurrence of irritation and rejection; the urinary catheter modified with the amino-functionalized multi-walled carbon nanotubes is not dependent on the release of the material in terms of antibacterial effect, avoiding the occurrence of potential toxicity and side effects.

[0031] In some embodiments, preferably, the preparation method of the nano coating comprises the following steps:

[0032] (1) after the urinary catheter is soaked in an organic solvent, it is rinsed with water;

[0033] (2) soaking the catheter after the step (1) in an aqueous solution of the amino-functionalized multi-walled carbon nanotubes and placing it in a horizontal shaker for shaking treatment, and then naturally air-drying after taking it out.

[0034] In the embodiment of the present application, the preparation method of the nano coating is preferred, the catheter is first soaked and treated to expand the catheter, the gap of the catheter is expanded, which is beneficial to the attachment of the amino-functionalized multi-walled carbon nanotubes on the surface of the catheter, and then the catheter is soaked in an aqueous solution of the amino-functionalized multi-walled carbon nanotubes and simultaneously subjected to shaking treatment on a shaker, which can make the amino-functionalized multi-walled carbon nanotubes fully contact with the catheter, be fixed on the surface of the catheter, and prevent the precipitation of the amino-functionalized multi-walled carbon nanotubes, so that the amino-functionalized multi-walled carbon nanotubes fully contact with the catheter and are more uniformly distributed on the surface of the catheter.

[0035] In some embodiments, preferably, in the step (1), the organic solvent comprises chloroform. Further preferably, in the step (1), the soaking treatment is performed for 3-4 min.

[0036] In the embodiment of the present application, the soaking treatment time is preferred, if the soaking time is too long, the catheter may be excessively expanded and cannot be retracted, and if the soaking time is too short, the catheter cannot be sufficiently expanded, which is not conducive to the attachment and fixation of the amino-functionalized multi-walled carbon nanotubes on the surface of the catheter.

[0037] In some embodiments, preferably, in the step (2), the concentration of the aqueous solution of the amino-functionalized multi-walled carbon nanotubes is 0.1-0.5 mg / mL.

[0038] In some embodiments, preferably, in the step (2), the shaking treatment is performed for 5-6 h. Further preferably, in the step (2), the frequency of shaking in the horizontal shaker is 80-100 rpm.

[0039] In the embodiment of the present application, the shaking treatment time is preferred, which can make the expanded catheter retract.

[0040] In some embodiments, preferably, in the step (2), before the natural air-drying, the catheter is first flushed with pure water.

[0041] In the embodiment of the present application, the catheter is first flushed with pure water before the natural air-drying, which can flush away the amino-functionalized multi-walled carbon nanotubes that are not fixed on the surface of the catheter, so as to avoid falling off during use and causing influence.

[0042] In some embodiments, preferably, the thickness of the nano coating is 5-10 μm.

[0043] In the embodiment of the present application, the thickness of the nano coating is preferably selected to improve the antibacterial effect of the nano coating. If the thickness is too large, the mechanical properties of the coating such as flexibility and wear resistance will be reduced, and the production cost will be increased. If the thickness is too small, the thin coating cannot provide sufficient antibacterial effect, which is not conducive to the improvement of the antibacterial effect. In addition, the thin coating has poor adhesion and is easy to fall off, which will affect the durability and stability of the coating.

[0044] The technical solutions of the present application will be described in detail below in combination with specific embodiments.

[0045] The amino-functionalized multi-walled carbon nanotubes used in the embodiment are prepared by the following method:

[0046] (a) Acyl chloride of carboxylated multi-walled carbon nanotubes: 1.0 g of MWNT-COOH (carboxylated multi-walled carbon nanotubes) is placed in a three-necked flask, 20 mL of dichlorosulfoxide is added, and stirring reflux is carried out in a 70℃ water bath for 24 h. The obtained mixed solution is filtered under reduced pressure with a polytetrafluoroethylene membrane, repeatedly washed with tetrahydrofuran (THF), and the obtained black powder-like solid is dried under vacuum at 50℃ to prepare MWNT-COCl;

[0047] (b) Amidation: MWNT-COCl is placed in a three-necked flask, 5 g of ammonium carbonate is added, and 50 mL of concentrated ammonia water is added dropwise. After stirring for 6 h, the solvent is removed by filtration, repeatedly washed with distilled water, and the obtained black powder-like substance is dried under vacuum at 50℃ to obtain MWNT-CONH2.

[0048] (c) Hofmann elimination reaction: MWNT-CONH2 prepared is placed in a three-necked flask, 30 mL of sodium hypochlorite is slowly added dropwise under ice bath conditions (0-5℃), and after 30 min, vigorous stirring is carried out for 4 h to complete the Hofmann elimination reaction. The obtained product is moved to a 70℃ water bath for continuous reaction for 2 h, filtered under reduced pressure after cooling, and repeatedly washed with distilled water to obtain a black powder-like substance, which is dried under vacuum to obtain MWNT-NH2 (amino-functionalized multi-walled carbon nanotubes).

[0049] Embodiment 1

[0050] (1) The urinary catheter is soaked in chloroform for 4 minutes. After the volume of the urinary catheter is slightly expanded, it is taken out and washed with a large amount of pure water.

[0051] (2) The urinary catheter treated in step (1) is soaked in an aqueous solution of amino-functionalized multi-walled carbon nanotubes with a concentration of 0.1 mg / mL, and is treated by shaking in a horizontal shaker at a shaking frequency of 90 revolutions per minute for 5 h. After being taken out, it is washed with pure water and naturally dried.

[0052] To prove that the nano coating of the amino-functionalized multi-walled carbon nanotube has good antibacterial effect, the following test is carried out. The test method is as follows: under the condition of 37℃, Staphylococcus aureus is cultured on the solid medium for 1h, then 0.1mg / mL amino-functionalized multi-walled carbon nanotube aqueous solution or 200mg / L silver nitrate aqueous solution is added dropwise on the surface, and the culture is continued for 48h, the number of bacteria on the culture medium is counted, and the culture medium without adding the amino-functionalized multi-walled carbon nanotube aqueous solution and the silver nitrate aqueous solution is used as a blank control group, and the results are shown in Table 1, wherein the dilution multiples of 10 -1 and 10 -2 respectively refer to dilution of the Staphylococcus aureus mother liquor by 10 times and 100 times. The photos after the amino-functionalized multi-walled carbon nanotube aqueous solution culture of the Staphylococcus aureus diluted by 10 times and 100 times are shown in Figure 1 and Figure 2 respectively, the photos after the silver nitrate aqueous solution culture of the Staphylococcus aureus diluted by 10 times and 100 times are shown in Figure 3 and Figure 4 respectively, and the photos after the blank control group culture of the Staphylococcus aureus diluted by 10 times and 100 times are shown in Figure 5 and Figure 6 respectively.

[0053] Table 1

[0054]

[0055] From the data in Table 1, it can be seen that after 48h of culture, the number of bacteria in the culture medium added with the amino-functionalized multi-walled carbon nanotube aqueous solution is obviously reduced compared with the blank control group, and the antibacterial effect is comparable to that of the silver nitrate aqueous solution.

[0056] In the present application, the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. mean that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In the present specification, the illustrative representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any appropriate manner in any one or more embodiments or examples. In addition, the different embodiments or examples described in the present specification and the features of the different embodiments or examples can be combined and combined by those skilled in the art without contradiction.

[0057] Although the above embodiments have been shown and described, it can be understood that the above embodiments are exemplary and cannot be understood as limiting the present application, and the changes, modifications, replacements and variations of the above embodiments made by those skilled in the art are within the protection scope of the present application.

Claims

1. A urinary catheter, characterized in that The whole catheter is covered with a nano coating prepared from an aqueous solution of aminated multi-walled carbon nanotubes.

2. The urinary catheter of claim 1, wherein, The preparation method of the nano coating comprises the following steps: (1) after soaking treatment of the catheter in an organic solvent, the catheter is washed with water; (2) the catheter treated in step (1) is soaked in an aqueous solution of aminated multi-walled carbon nanotubes and is subjected to oscillation treatment in a horizontal shaking table, and is naturally dried after being taken out.

3. The urinary catheter of claim 2, wherein, In step (1), the organic solvent comprises chloroform.

4. The urinary catheter according to claim 2 or 3, characterized in that In step (1), the soaking treatment time is 3-4 min.

5. The urinary catheter of claim 2, wherein, In step (2), the concentration of the aqueous solution of aminated multi-walled carbon nanotubes is 0.1-0.5 mg / mL.

6. The urinary catheter according to claim 2 or 5, characterized in that In step (2), the oscillation treatment time is 5-6 h.

7. The urinary catheter of claim 6, wherein, In step (2), the oscillation frequency in the horizontal shaking table is 80-100 rpm.

8. The urinary catheter of claim 2, wherein, In step (2), before natural drying, the catheter is first washed with pure water.

9. The urinary catheter of claim 1, wherein, The thickness of the nano coating is 5-10 μm.

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