New use and method of a tetrahydro-beta-carboline derivative for promoting the color change of crop fruits

Abstract

This invention relates to the field of fruit color-changing technology, specifically to a novel application and method of a tetrahydroβ-carboline derivative for promoting fruit color-changing in crops. The tetrahydroβ-carboline derivative is specifically (1S,3S)-N'-(4-chlorobenzylmethyl)-1-methyl-2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indole-3-carbohydrazide. The tetrahydroβ-carboline derivative can promote fruit color-changing, allowing fruits to be harvested earlier, improving fruit quality, and increasing economic benefits. Furthermore, the tetrahydroβ-carboline derivative used in this invention is relatively safe for fruits and has not caused any adverse effects.

Description

Technical Field

[0001] This invention relates to the field of fruit color-changing technology, specifically to a novel use and method of a tetrahydroβ-carbazoline derivative to promote crop fruit color-changing. Background Technology

[0002] In the cultivation and management of crops such as grapes, strawberries, tomatoes, citrus fruits, cherries, apples, dragon fruit, and peaches, many factors influence prices, primarily the time of market and quality. Fruit quality determines price; different qualities of fruit can have vastly different prices. The color and appearance of the fruit are crucial factors in evaluating its quality. Promoting fruit color development and sugar content is one way to improve fruit quality. In production, certain products are often used to help increase the sweetness and color of the fruit. Using coloring agents not only accelerates and evenly colores the fruit but also allows for earlier market entry, increasing farmers' income and creating higher economic value. Therefore, farmers have a significant demand for color-enhancing products.

[0003] Although there are many color-changing products on the market, the market is flooded with products of varying quality and additives, making it difficult to distinguish the quality of color-changing products.

[0004] For example, there are many types of grape color-changing products on the market. Based on their color-changing principles, they can be divided into two categories: one is nutrient-based, such as potassium sulfate, manganese sulfate, amino acid fertilizers, and bio-fertilizers, which mainly rely on supplementing nutrients to promote grape color change; the other is hormone-based, such as S-inducer and ethephon, which rely on regulating the growth and development process of grapes to accelerate coloring. The advantage of the first type of substance is its safety, and it can also improve fruit quality to some extent, but its disadvantage is that the color-changing speed is slow. In the second type, S-inducer and ethephon can easily cause leaf and fruit drop and shorten the fruit's storage period, while abscisic acid is unstable and easily decomposes in light, thus its use is subject to higher barriers to entry.

[0005] (1S,3S)-N'-(4-chlorobenzylmethyl)-1-methyl-2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indole-3-carboxylhydrazine is a novel antiviral drug developed as a plant antiviral agent. In tomato experiments, we unexpectedly discovered that spraying (1S,3S)-N'-(4-chlorobenzylmethyl)-1-methyl-2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indole-3-carboxylhydrazine not only promoted tomato fruit growth but also accelerated fruit color change. Inspired by this phenomenon, we attempted to use (1S,3S)-N'-(4-chlorobenzylmethyl)-1-methyl-2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indole-3-carboxylhydrazine to promote grape color change, and the experiment ultimately achieved excellent results. Summary of the Invention

[0006] To address the technical problems of existing fruit color-changing products, such as slow color-changing speed, easy leaf and fruit drop, shortened fruit storage period, or instability, this invention provides a new application and method for promoting crop fruit color-changing using a tetrahydroβ-carboline derivative. After treating crops with the tetrahydroβ-carboline derivative, the color-changing speed and effect of the fruit are significantly improved, allowing tomatoes and grapes to be harvested earlier, increasing economic benefits. Furthermore, this product is relatively safe for tomatoes and grapes.

[0007] First aspect of the invention:

[0008] The use of a tetrahydroβ-carboline derivative to promote fruit color change in crops, wherein the tetrahydroβ-carboline derivative is specifically (1S,3S)-N'-(4-chlorobenzylmethyl)-1-methyl-2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indole-3-carbohydrazide.

[0009] As a preferred option, the concentration of the tetrahydroβ-carbazoline derivative is 0.05-1.0 g / L.

[0010] As a preferred option, the concentration of the tetrahydroβ-carbazoline derivative is 0.06-0.25 g / L.

[0011] As a preferred option, the crop is grapes or tomatoes.

[0012] Second aspect of the invention:

[0013] A method for promoting fruit color change in crops involves treating crops with a tetrahydroβ-carboline derivative, specifically (1S,3S)-N'-(4-chlorobenzylmethyl)-1-methyl-2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indole-3-carbohydrazide.

[0014] As a preferred option, tetrahydroβ-carboline derivatives are applied during the fruit expansion and / or color-changing stages of the crop.

[0015] As a preferred method, the pesticide is applied to the crop ears, leaves, branches, or the entire crop. Further, the application method is spraying.

[0016] As a preferred option, the concentration of the tetrahydroβ-carbaline derivative is 0.05-0.1.0 g / L.

[0017] As a preferred option, the concentration of the tetrahydroβ-carbazoline derivative is 0.06-0.25 g / L.

[0018] As a preferred option, formulations made from tetrahydroβ-carboline derivatives and agriculturally acceptable adjuvants are used to treat crops.

[0019] Furthermore, the formulation of the tetrahydroβ-carbamoline derivative is selected from one or more pesticide formulations such as emulsifiable concentrates, water-in-oil emulsions, suspension concentrates, wettable powders, water-dispersible granules, soluble liquids, soluble granules, and microemulsions, but is not limited to the formulations mentioned above.

[0020] Furthermore, the tetrahydroβ-carbamate derivative is an emulsifiable concentrate formulation and also includes one or more pesticide-acceptable adjuvants such as cyclohexanone, N,N-dimethylpyrrolidone, N,N-dimethylformamide, N,N-dimethylacetamide, dimethyl sulfoxide, ethanol, octylphenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, phenethylphenol formaldehyde ester polyoxyethylene ether, styrene-based formaldehyde resin polyoxyethylene-polyoxypropylene block polyether, calcium dodecylbenzenesulfonate, C10 solvent, xylene, and 200# solvent oil.

[0021] Furthermore, the tetrahydroβ-carbamoline derivative is an emulsion formulation and also includes one or more pesticide-acceptable adjuvants such as C10 solvent, toluene, xylene, octylphenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, phenethylphenol formaldehyde ester polyoxyethylene ether, octylphenol formaldehyde resin polyoxyethylene ether, ethylene glycol, and water.

[0022] Furthermore, the tetrahydroβ-carbamate derivative is a suspension formulation and also includes one or more pesticide-acceptable adjuvants such as sodium methylene bisnaphthalene sulfonate, styrene-phenol formaldehyde resin polyoxyethylene-polyoxypropylene block polyether, separating powder, alkylphenol polyoxyethylene ether, naphthalene sulfonate formaldehyde condensate, alcohol ether phosphate, polycarboxylate, ethylene glycol, glycerol, Kathon, magnesium aluminum silicate, silica, xanthan gum, organosilicon defoamer, and water.

[0023] Furthermore, the tetrahydroβ-carbamate derivative is a wettable powder formulation and also includes one or more pesticide-acceptable adjuvants such as splitting powder, sodium dodecylbenzene sulfonate, naphthalene sulfonate formaldehyde condensate, sodium lignosulfonate, polycarboxylate, silica, kaolin, and diatomaceous earth.

[0024] Furthermore, the tetrahydroβ-carbamate derivative is a water-dispersible granule formulation, and also includes one or more pesticide-acceptable adjuvants such as naphthalene sulfonate formaldehyde condensate, polycarboxylate, sodium dodecylbenzene sulfonate, sodium lignosulfonate, calcium lignosulfonate, phenethylphenol polyoxyethylene sulfate sodium sulfate, sodium polyacrylate, dextrin, oxidized corn starch, ammonium sulfate, sodium gluconate, light calcium carbonate, kaolin, and silica.

[0025] Furthermore, the tetrahydroβ-carbamoline derivative is a soluble formulation and also includes one or more pesticide-acceptable adjuvants such as cyclohexanone, N,N-dimethylpyrrolidone, N,N-dimethylformamide, N,N-dimethylacetamide, ethanol, octylphenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, phenethylphenol formaldehyde ester polyoxyethylene ether, styrene-based formaldehyde resin polyoxyethylene-polyoxypropylene block polyether, calcium dodecylbenzenesulfonate, and water.

[0026] Furthermore, the tetrahydroβ-carbamate derivative is a soluble granular formulation and also includes one or more pesticide-acceptable adjuvants such as naphthalene sulfonate formaldehyde condensate, polycarboxylate, sodium dodecylbenzene sulfonate, sodium lignosulfonate, calcium lignosulfonate, phenethylphenol polyoxyethylene sulfate sodium sulfate, sodium polyacrylate, dextrin, ammonium sulfate, sodium gluconate, and xanthan gum.

[0027] Furthermore, the tetrahydroβ-carbamoline derivative is a microemulsion formulation and also includes one or more pesticide-acceptable adjuvants such as cyclohexanone, N,N-dimethylpyrrolidone, N,N-dimethylformamide, N,N-dimethylacetamide, octylphenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, phenethylphenol polyoxyethylene ether, octylphenol formaldehyde resin polyoxyethylene ether, ethylene glycol, water, and water-soluble defoamers.

[0028] Furthermore, the crops are grapes and / or tomatoes.

[0029] The beneficial effects of this invention are as follows:

[0030] This invention can promote fruit color change, allowing the fruit to be marketed earlier, improving fruit quality, and increasing economic benefits. Furthermore, the tetrahydroβ-carboline derivative used in this invention is relatively safe for the fruit and has not caused any adverse effects. Detailed Implementation

[0031] To enable those skilled in the art to better understand the technical solutions of this invention, the technical solutions in the embodiments of this invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of this invention, and not all embodiments. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without creative effort should fall within the scope of protection of this invention.

[0032] The tetrahydroβ-carbamate derivative used in the specific embodiments of this invention is (1S,3S)-N'-(4-chlorobenzylmethyl)-1-methyl-2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indole-3-carbamoylhydrazine, with the following structural formula:

[0033] .

[0034] The 10% S-inducer soluble concentrate used in the specific embodiments of the present invention was purchased from Sichuan Runer Technology Co., Ltd.

[0035] Example 1: 10% Tetrahydroβ-carboline Derivative Suspension

[0036] By weight percentage, the formulation includes 10% tetrahydroβ-carboline derivative, 2% sodium methylene bisnaphthalene sulfonate, 3% styrene-phenol-formaldehyde resin polyoxyethylene-polyoxypropylene block polyether, 5% ethylene glycol, 0.2% Kathon, 1% magnesium aluminum silicate, 2% silica, 0.15% xanthan gum, 0.3% silicone defoamer, and water to 100%.

[0037] After mixing the above materials, they are added to a sand mill for grinding to obtain a 10% tetrahydroβ-carboline derivative suspension.

[0038] Example 2: 10% Tetrahydroβ-carbaline derivative wettable powder

[0039] By weight percentage, the formula includes 10% tetrahydroβ-carboline derivative, 3% stretching powder, 6% naphthalene sulfonate formaldehyde condensate, 3% sodium lignosulfonate, 15% silica, and kaolin to make up 100%.

[0040] The above materials are coarsely pulverized in proportion and then mixed evenly in a mixer. After being pulverized by air jet milling, a 10% tetrahydroβ-carbamoline derivative wettable powder is obtained.

[0041] Example 3: 10% Tetrahydroβ-carboline Derivative Emulsifiable Oil

[0042] By weight percentage, the formulation includes 10% tetrahydroβ-carboline derivative, 5% cyclohexanone, 10% N,N-dimethylpyrrolidone, 5% octylphenol polyoxyethylene ether, 5% fatty alcohol polyoxyethylene ether, 1% phenethylphenol formaldehyde ester polyoxyethylene ether, 4% calcium dodecylbenzenesulfonate, and C10 is made up to 100%.

[0043] The above materials are added into a mixing vessel and heated to 40±5℃ to fully dissolve and mix evenly. After filtration, a 10% tetrahydroβ-carboline derivative emulsion is obtained.

[0044] Example 4: 10% Tetrahydroβ-carbaline derivative water-dispersible granules

[0045] By weight percentage, the formulation includes 10% tetrahydroβ-carboline derivative, 5% naphthalene sulfonate formaldehyde condensate, 5% polycarboxylate, 2% sodium dodecylbenzene sulfonate, 5% calcium lignosulfonate, 10% oxidized corn starch, and ammonium sulfate to bring the total to 100%.

[0046] The above materials are mixed, pulverized by ultra-micro airflow, extruded and granulated, dried at 60°C, and sieved to obtain 10% tetrahydroβ-carboline derivative water-dispersible granules.

[0047] Example 5: 10% Tetrahydroβ-carboline derivative microemulsion

[0048] By weight percentage, the formulation includes 10% tetrahydroβ-carboline derivative, 10% cyclohexanone, 15% N,N-dimethylformamide, 10% phenethylphenol polyoxyethylene ether, 5% fatty alcohol polyoxyethylene ether, 5% ethylene glycol, 0.2% water-soluble defoamer, and deionized water to make up to 100%.

[0049] Tetrahydroβ-carboline derivative, cyclohexanone, N,N-dimethylformamide, phenethylphenol polyoxyethylene ether, and fatty alcohol polyoxyethylene ether were thoroughly stirred and heated until completely dissolved. Then, ethylene glycol, silicone defoamer, and deionized water were added, and the mixture was stirred and mixed until clear and transparent to obtain a 10% tetrahydroβ-carboline derivative microemulsion.

[0050] Experimental Example 1: Effect of Tetrahydroβ-carbazoline Derivative Spraying on Tomato Plants and its Color Change

[0051] The experiment was conducted in Pangjia Town, using the Maofen tomato variety. The tomato seedlings were purchased from a nursery and cultivated for 35 days before transplanting on September 15, 2022. All tomato cultivation conditions within the greenhouse (soil type, cultivation substrate, organic matter content, pH, soil moisture, fertility, tillage, and disinfection methods, etc.) were consistent and aligned with local tomato planting practices. The tomato fruits were sprayed during the early fruit expansion to color-changing stages.

[0052] Experimental groups 1-5 were sprayed with a 750-fold dilution of the formulations from Examples 1-5, respectively;

[0053] Control group 1 was sprayed with a 500-fold dilution of 10% S-inducer soluble solution;

[0054] Control group 2 was sprayed with an equal amount of water;

[0055] Each treatment was sprayed with 10 plants, and the treatment was repeated 4 times.

[0056] During application, each treatment was diluted with the corresponding pesticide solution, using 30L of solution per acre as the standard. The solution was sprayed onto the leaves, branches, and fruit surfaces of the tomato plants using a sprayer, ensuring that water droplets did not pool and fall. The control group 2 was also sprayed with 30L of water per acre. The treatments were applied twice: once during the fruit expansion stage (November 10, 2022) and once during the color-changing stage (November 23, 2022).

[0057] Fruit circumference was measured on the day of each spraying and seven days after the second spraying. Two fruits were randomly selected from the same location on each tomato plant for circumference measurement, with a total of 80 fruits measured for each treatment. The average circumference change and the magnitude of increase or decrease after each application were calculated using the following formula:

[0058] Increase / decrease (%) = (mean circumference change after treatment in the treatment group - mean circumference change after 2 days of treatment in the control group) / mean circumference change after 2 days of treatment in the control group × 100%.

[0059] The color change of the fruit was recorded on the day of the first spray and seven days after the second spray. The tomatoes with different color changes were graded visually and divided into six grades: "-", "+", "++", "+++", "++++", and "+++++". The grading criteria are as follows:

[0060] "-": The fruit has not changed color at all;

[0061] "+": The area of ​​fruit color change accounts for less than 20% of the total fruit surface area;

[0062] "++": The area of ​​fruit color change accounts for 20%-40% of the total fruit surface area. 20%-40% means excluding 20% ​​and including 40%, and the same applies below.

[0063] "+++": The area of ​​fruit color change accounts for 40%-60% of the total fruit surface area;

[0064] "++++": The area of ​​fruit color change accounts for 60%-80% of the total fruit surface area;

[0065] "+++++": The color-changing area of ​​each fruit accounts for 80%-100% of the total fruit surface area, meaning that the fruit has basically completely changed color.

[0066] Fruit yield was investigated on the day of the first spray and seven days after the second spray. Two fruits were randomly selected from the same location on each tomato plant and weighed. A total of 80 fruits were harvested for each treatment, and the weight of each fruit was calculated.

[0067] Based on statistical analysis, the data on the perimeter, color change, and weight of tomato fruits are shown in Tables 1-4 below.

[0068] Table 1. Statistical Analysis of Tomato Fruit Circumference and Circumference Variation (Unit: cm)

[0069]

[0070] Table 1 shows that tetrahydroβ-carboline derivatives have a significant promoting effect on increasing the perimeter of tomato fruits, basically reaching or exceeding the effect of the commonly used growth regulator S-inducer. Seven days after the second application, the fruit perimeter of experimental group 2 was the longest. However, considering that the initial perimeter of experimental group 2 was also the longest, the difference between the two perimeter measurements was used as the main reference. Among the two perimeter differences, the perimeter change of experimental group 1 was the largest, increasing by 35.71% compared to control group 2; followed by experimental group 2, with a perimeter change increasing by 28.57% compared to control group 2.

[0071] Table 2. Statistical Analysis of Tomato Fruit Color Change Before Spraying

[0072]

[0073] Table 3. Statistical Analysis of Tomato Fruit Color Change Seven Days After the Second Application

[0074]

[0075] As shown in Tables 2 and 3, tetrahydroβ-carboline derivatives have a significant promoting effect on tomato fruit color change, basically reaching or exceeding the effect of commonly used growth regulator S-inducer. Among them, experimental group 1 showed the best color change effect, with 87.5% of the fruits fully color-changing. Experimental group 2 also showed a good color change effect, with 85% of the fruits fully color-changing. The proportion of fully color-changing fruits in the other experimental groups also reached over 60%, which was better than control group 2 (treated with water), demonstrating a certain color-promoting effect.

[0076] Table 4. Statistical Analysis of Tomato Fruit Weight (Unit: g)

[0077]

[0078] Table 4 shows that tetrahydroβ-carboline derivatives have a significant promoting effect on tomato fruit yield, basically reaching or exceeding the effect of the commonly used growth regulator S-inducer. Among all treatment groups, experimental group 1 had the largest tomato fruit weight, at 221.54g, an increase of 27.04% compared to control group 2; followed by experimental group 2 and control group 1, at 197.64g and 195.67g respectively, representing increases of 13.34% and 12.21% compared to control group 2.

[0079] In summary, treating tomatoes with tetrahydroβ-carboline derivatives can effectively increase the circumference and weight of the fruit and promote fruit color change without adversely affecting the fruit. The effect is best achieved with a 10% tetrahydroβ-carboline derivative suspension diluted 750 times. S-inducer is a common growth regulator often used as a fruit color-changing agent. While it can promote fruit color change, its effect on increasing fruit yield is relatively weak. Furthermore, inducer has a certain ripening effect, leading to problems such as fruit drop, soft fruit, fruit fall, and difficulty in storage and transportation. It can also cause premature yellowing and drying of leaves. The color-changing effect of tetrahydroβ-carboline derivatives is comparable to or even better than that of S-inducer, without affecting normal plant growth, and does not cause problems such as fruit drop, soft fruit, fruit fall, difficulty in storage and transportation, or premature yellowing and drying of leaves. Therefore, it can be used as a color-changing agent to promote tomato fruit color change.

[0080] Experimental Example 2: Effect of spraying grape bunches with tetrahydroβ-carboline derivatives on grape color change.

[0081] The experiment was conducted in Pangjia Town, using the Muscat grape variety. All grape cultivation conditions within the greenhouse (soil type, cultivation substrate, organic matter content, pH, soil moisture, fertility, tillage and disinfection methods, etc.) were consistent and in line with local grape growing practices. The grapes were sprayed with the solution during the early stages of color change.

[0082] Experimental groups 1-3 were sprayed with the formulation of Example 1 at dilutions of 1500, 750, and 500 times, respectively.

[0083] Control group 1 was sprayed with a 500-fold dilution of 10% S-inducer soluble solution;

[0084] Control group 2 was sprayed with an equal amount of water;

[0085] Each treatment was sprayed with 10 plants, and the treatment was repeated 4 times.

[0086] When applying, dilute the corresponding solution according to the different dosages of tetrahydro-β-carboline derivative suspension, using 30L of solution per acre as the standard, and spray evenly on all surfaces of the grape bunches using a sprayer. Spraying was carried out on July 15, 2022. After spraying, fruits with the same color change were selected from each treatment group and marked, with 20 bunches of fruit marked in each treatment group, and the process was repeated 3 times.

[0087] The color change of the grapes was investigated seven days (July 22) and fourteen days (July 29) after spraying. The grapes with different color changes were graded by visual inspection, and the grading criteria were the same as in Experiment 1.

[0088] After the investigation was completed, the color change status of each treatment group was analyzed, and the results are shown in Table 5-7.

[0089] Table 5. Statistical Analysis of Grape Fruit Color Change Before Spraying

[0090]

[0091] Table 6. Statistical Analysis of Grape Fruit Color Change in the First Survey After Application of Pesticides

[0092]

[0093] As shown in Table 6, seven days after drug administration, the color change level of experimental group 2, which used 750 times diluted 10% tetrahydroβ-carboline derivative suspension, was the highest, significantly higher than that of experimental group 1, which used 1500 times diluted 10% tetrahydroβ-carboline derivative suspension, and experimental group 3, which used 500 times diluted 10% tetrahydroβ-carboline derivative suspension. It was also significantly higher than that of control group 1 and control group 2.

[0094] Table 7. Statistical Analysis of Grape Fruit Color Change in the Second Post-Treatment Survey

[0095]

[0096] As shown in Table 7, fourteen days after treatment, the color change of grapes in all treatment groups (experimental groups 1-3) using Example 1 was significantly higher than that in control group 1 and control group 2; among them, the color change of grapes in experimental group 2 (750 times dilution of 10% tetrahydroβ-carboline derivative suspension) and experimental group 3 (500 times dilution of 10% tetrahydroβ-carboline derivative suspension) was significantly different from that in control group 2.

[0097] In summary, treatment with tetrahydroβ-carboline derivatives significantly promotes grape ripening. Spraying grape bunches with a 10% tetrahydroβ-carboline derivative suspension at a dilution of 1500-500 times is more effective than applying a 10% S-inducer soluble concentrate. Considering both cost and duration of efficacy, a 750-fold dilution is preferred for treatment at the beginning of the grape ripening period.

[0098] Experimental Example 3: Effect of spraying the entire grape plant with tetrahydroβ-carboline derivative on grape color change.

[0099] The grape variety used was Jinxin No. 6, a late-maturing grape variety. The spraying time was at the early stage of grape color change. The test site was located in Lijin County, Binzhou City, Shandong Province. All grape cultivation conditions in the greenhouse (soil type, cultivation substrate, organic matter content, pH, soil moisture, fertility, cultivation and disinfection methods, etc.) were consistent and in line with local grape planting practices.

[0100] The formulations of Example 1, applied at 1500, 750, and 500 times dilution, were set as test groups 1-3. The 10% S-inducer soluble concentrate applied at 500 times dilution was set as control group 1, and the solution applied with water was set as control group 2.

[0101] When applying the pesticide, dilute the pesticide solution according to the different treatments and dosages set. Spray the entire grapevine with a sprayer at a standard rate of 30L of pesticide solution per acre. Spraying was carried out on September 22, 2022. After spraying, select fruits with the same color change status for each treatment and mark them. Mark 20 bunches of fruit for each treatment, and repeat three times.

[0102] The color change of the fruit was investigated on October 13 and October 26. The investigation and statistical methods were the same as in Example 1.

[0103] Table 8. Grape color change status survey before the experiment (September 22)

[0104]

[0105] Table 9. Grape color change after the first treatment (October 13th)

[0106]

[0107] As shown in Table 9, in the first post-treatment survey, the color change levels of all experimental groups 1-3 and control group 1 were better than those of the water control group; among them, the color change level of experimental group 3 treated with 500 times dilution was the highest, significantly higher than that of the water control group.

[0108] Table 10 Grape color change after the second treatment (October 26)

[0109]

[0110] As shown in Table 10, in the second post-treatment survey, the color change level of all treatment groups in Example 1 (experimental groups 1-3) and control group 1 was better than that of the water control group; among them, the best color change effect was achieved by the 500x experimental group 3 and the 750x experimental group 2, while the 1500x experimental group 1 and control group 1 had basically the same effect.

[0111] The analysis results of the two surveys show that spraying the entire grape vine with the treatment groups 1-3 during the early stage of color change can accelerate the color change of the late-maturing variety Jinxin No. 6 grape.

[0112] In conclusion, spraying the entire grape vine with tetrahydroβ-carboline derivatives can achieve a good color-changing effect.

[0113] Although the present invention has been described in detail by way of preferred embodiments, the present invention is not limited thereto. Various equivalent modifications or substitutions can be made to the embodiments of the present invention by those skilled in the art without departing from the spirit and essence of the invention, and such modifications or substitutions should all be within the scope of the present invention. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in the present invention should also be covered within the protection scope of the present invention.

Claims

1. The use of a tetrahydroβ-carboline derivative in promoting fruit color change in crops, characterized in that, The tetrahydroβ-carbamate derivative is specifically (1S,3S)-N'-(4-chlorobenzylmethyl)-1-methyl-2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indole-3-carbamoylhydrazine.

2. The use as described in claim 1, characterized in that, The concentration of tetrahydroβ-carbazoline derivatives used is 0.05-1.0 g / L.

3. The use as described in claim 1, characterized in that, The concentration of tetrahydroβ-carboline derivatives used is 0.06-0.25 g / L.

4. The use as described in claim 1, characterized in that, The crop in question is either grapes or tomatoes.

5. A method for promoting fruit color change in crops, characterized in that, Crops are treated with a tetrahydroβ-carboline derivative, specifically (1S,3S)-N'-(4-chlorobenzylmethyl)-1-methyl-2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indole-3-carbohydrazide.

6. The method as described in claim 5, characterized in that, Apply tetrahydro-β-carboline derivatives during the fruit expansion and / or color-changing stages of crops.

7. The method as described in claim 5 or 6, characterized in that, Apply pesticides to the ears, leaves, branches, or the entire plant.

8. The method as described in claim 5, characterized in that, The concentration of the tetrahydroβ-carbazoline derivative is 0.05-1.0 g / L.

9. The method as described in claim 5, characterized in that, The concentration of tetrahydroβ-carbazoline derivatives is 0.06-0.25 g / L.

10. The method as described in claim 5, characterized in that, Formulations made from tetrahydro-β-carboline derivatives and agriculturally acceptable adjuvants are used to treat crops.

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