An innovative blood product processing and dispensing method

Abstract

The application discloses a kind of innovative blood product processing and subpackaging method in the technical field of medical devices, step 1: blood raw materials are centrifuged and separated by processing device, so that plasma and blood cells are separated;Step 2: add low-temperature ethanol to the plasma to precipitate, extract plasma protein in the plasma;Step 3: select a plurality of pore sizes corresponding to plasma protein and blood cells Sterile filter membrane, terminal filtration is carried out to plasma protein and blood cells using sterile filter membrane, and plasma protein and blood cells after filtration are subpackaged, to obtain the blood product, to solve the existing problems in the prior art to a certain extent The fixed position of the discharge port is set, and in actual use, due to the fact that the number of various types of blood cells in blood is mostly different, the fixed position of the discharge port can cause the mixed target blood cells and the upper or lower blood cells, affecting the quality of subsequent finished products.

Description

Technical Field

[0001] This invention belongs to the field of medical device technology, specifically an innovative method for processing and packaging blood products. Background Technology

[0002] Blood products refer to the collective term for plasma protein components and formed elements of blood cells produced from the plasma of healthy individuals or specifically immunized individuals through separation, purification, or recombinant DNA technology. Current technologies primarily employ centrifugation, low-temperature ethanol separation, and pressure filtration processes. However, industrial centrifuges, used for centrifugation, cannot separate the separated blood layers during operation. Furthermore, directly aspirating the different liquid layers using pipettes easily leads to mixing during the aspiration process.

[0003] To address the aforementioned issues, Chinese Patent Publication No. CN110064529B discloses an automatic dispensing medical blood centrifuge, comprising a detachably arranged centrifugation device and a liquid-filling component. The liquid-filling component includes a test tube assembly for holding blood and a dispensing mechanism for extracting the blood layer by layer after centrifugation. The test tube assembly is detachably inserted into the middle of the dispensing mechanism. The test tube assembly and the dispensing mechanism are configured to switch between an isolated state and a connected state, with the initial state being the isolated state. The centrifugation device includes an automatic control device for switching the test tube assembly and the dispensing mechanism from the isolated state to the connected state. The centrifugation device includes a vertically arranged rotating mechanism. A clamping mechanism for mounting the liquid-filling component is located at an eccentric position on the top of the rotating mechanism, and a shock-absorbing mechanism for buffering it is located at the bottom of the rotating mechanism. The test tube assembly includes... The device comprises an outer tube and an inner tube that are combined with each other. The outer tube is an upward-opening tube structure, and the inner tube is an open-end tube structure. The inner tube is embedded inside the outer tube, and the two form a sealed sliding connection. An external step three is coaxially provided on the outer circumference of the outer tube, and the external step three is close to the opening of the outer tube. The outer circumference of the outer tube has discharge port one, discharge port two, discharge port three, and discharge port four, which are spirally arranged from bottom to top. Discharge port one is used to discharge the red blood cell layer and corresponds to the bottom of the red blood cell layer; discharge port two is used to discharge the cytokine layer and corresponds to the bottom of the white blood cell layer; discharge port three is used to discharge the platelet layer and corresponds to the bottom of the platelet layer; and discharge port four is used to discharge the plasma layer and corresponds to the bottom of the plasma layer.

[0004] The aforementioned device solves the problem in existing technologies where, after centrifugation, the blood layers need to be aspirated using a pipette, which can easily cause mixing of the liquid layers. However, the device has a fixed discharge port position. In actual use, since the number of different types of blood cells varies greatly, the fixed discharge port position may cause the discharged target blood cells to mix with the blood cells in the upper or lower layers, affecting the quality of the subsequent finished product. Therefore, it is necessary to propose an innovative blood product processing and packaging method to solve, to some extent, the problem in existing technologies where the fixed discharge port position may cause the discharged target blood cells to mix with the blood cells in the upper or lower layers, affecting the quality of the subsequent finished product. Summary of the Invention

[0005] The purpose of this invention is to provide an innovative method for processing and packaging blood products, which can, to a certain extent, solve the problem in the prior art where the discharge port is fixed in position. In actual use, since the number of various types of blood cells in the blood is mostly different, the fixed position of the discharge port may cause the discharged target blood cells to mix with the blood cells in the upper or lower layers, affecting the quality of the subsequent finished products.

[0006] To achieve the above objectives, the technical solution of the present invention is as follows: an innovative method for processing and packaging blood products, comprising:

[0007] Step 1: The blood raw material is centrifuged and separated using a processing device to separate the plasma and blood cells;

[0008] Step 2: Add low-temperature ethanol to the plasma to precipitate and extract plasma proteins;

[0009] Step 3: Select several sterile filter membranes with pore sizes corresponding to plasma proteins and blood cells, use the sterile filter membranes to perform terminal filtration of plasma proteins and blood cells, and then dispense the filtered plasma proteins and blood cells to obtain the blood products.

[0010] Furthermore, the processing device includes a housing, with a centrifugal assembly and a water bath on the bottom wall of the housing. The centrifugal assembly includes a base, with a chamber inside the base. A drive motor is fixedly connected to the bottom wall of the chamber, and a connecting shaft is coaxially fixedly connected to the output shaft of the drive motor. The connecting shaft extends through the top wall of the chamber to the outside of the chamber and is rotatably engaged with the top wall of the chamber. A connecting seat is fixedly connected to the end of the connecting shaft away from the drive motor. A centrifugal tube is detachably connected to the centrifugal assembly. An inverted U-shaped outlet tube is fixedly connected to the top wall of the housing. One end of the outlet tube extends into the centrifugal tube, and the other end of the outlet tube is connected to a collection tube. A first one-way valve is fixedly connected to the inner side wall of the outlet tube near the collection tube. The collection tube is placed in the water bath. Several first openings are opened on the side wall of the outlet tube inside the centrifugal tube, and the first openings are arranged vertically. A first solenoid valve is fixedly connected to the side wall of each of the first openings. An air bladder is connected to the side wall of the outlet tube, and a second solenoid valve is fixedly connected to the connection. A second opening is opened at the top of the outlet tube, and a third solenoid valve is fixedly connected to the side wall of the second opening.

[0011] It also includes a control system, which consists of a camera and a controller; the camera is used to capture images of the centrifuged blood material inside the centrifuge tube; the controller is used to control the operation of the first and third solenoid valves based on the image information.

[0012] Beneficial effects: When using this device, blood raw materials are placed in centrifuge tubes, the drive motor is started, and the centrifuge tubes are centrifuged, causing plasma, platelets, red blood cells, and white blood cells to separate into corresponding centrifuged layers under the action of centrifugal force. Then, the first solenoid valve is kept closed, and the second solenoid valve is opened. By squeezing the air bladder, the air pressure in the outlet tube decreases, and the device is then activated. The camera continuously collects images of each centrifuged layer in the centrifuge tube. The operator opens the first solenoid valve corresponding to the desired blood product layer, and the target liquid of that layer enters the outlet tube and then the collection tube. The target liquid continuously flows from the outlet tube into the collection tube. The upper centrifuged layer corresponding to the target liquid moves downwards. When the upper centrifuged layer is about to reach the... When the first solenoid valve is nearly opened, the controller activates the third solenoid valve, causing the liquid near the centrifuge tube to fall back into the tube. This ensures that some of the target liquid remains in the centrifuge tube, guaranteeing that the extracted liquid is indeed the target liquid and improving the extraction accuracy of the device. The design of the first solenoid valve and other components makes the device less susceptible to the influence of the number of blood cells in the blood raw material. It enables more precise collection of different centrifugation layers and solves the problem of fixed discharge port positions in existing technologies. In actual use, because the number of different types of blood cells varies, a fixed discharge port may cause the discharged target blood cells to mix with the upper or lower layers, affecting the quality of the final product.

[0013] Furthermore, the side wall of the outlet tube is connected to a liquid storage tube, which contains an anticoagulant.

[0014] Beneficial effects: When using this device, the anticoagulant required for the target liquid is added to the storage tube. As the target liquid moves in the outlet tube, it drives the gas at the connection between the storage tube and the outlet tube, causing the gas pressure at that point to drop. The anticoagulant in the storage tube enters the outlet tube and mixes with the target liquid, thereby reducing the need for subsequent anticoagulant addition steps and improving the steps for operators to process blood products.

[0015] Furthermore, a second check valve is fixedly connected at the connection between the outlet tube and the storage tube.

[0016] Beneficial effects: During the use of this device, the second one-way valve can, to a certain extent, prevent the target liquid in the outlet tube from entering the storage tube, thus reducing the yield of the target blood products.

[0017] Furthermore, sound-absorbing panels made of polyester fiber are fixedly connected to both the side walls and the top wall of the chamber.

[0018] Beneficial effects: When using this device, the sound-absorbing panel can reduce the impact of vibration generated by the drive motor on the operator's ears, thus improving the user experience.

[0019] Furthermore, the water bath is equipped with an inner bag and a temperature sensor. A semiconductor cooling chip is fixedly connected to the inner wall of the inner bag. The temperature sensor is used to collect temperature information from the collection tube. The controller controls the semiconductor cooling chip to work based on the temperature information and image information.

[0020] Beneficial effects: When using this device, the controller determines the target liquid type corresponding to the collected centrifuged layer based on the image information, and adjusts the set temperature of the water bath in the collection tube according to the target liquid type. The controller adjusts the temperature of the water bath by controlling the semiconductor cooling chip to cool or heat, so that the temperature of the water bath can change according to the different storage temperatures of different blood products, reducing the probability of deterioration during the blood product dispensing process. Attached Figure Description

[0021] Figure 1 This is a flowchart illustrating an embodiment of an innovative blood product processing and packaging method of the present invention;

[0022] Figure 2 This is a cross-sectional view of a processing apparatus according to an embodiment of an innovative blood product processing and packaging method of the present invention;

[0023] Figure 3 This is a circuit diagram of a processing apparatus according to an embodiment of an innovative blood product processing and packaging method of the present invention. Detailed Implementation

[0024] The following detailed description illustrates the specific implementation method:

[0025] The reference numerals in the accompanying drawings include: outer casing 1, centrifuge assembly 2, base 21, sound-absorbing plate 22, drive motor 23, connecting shaft 24, connecting seat 25, centrifuge tube 3, outlet tube 4, first solenoid valve 41, third solenoid valve 42, air bag 5, liquid storage tube 6, collection tube 7, water bath 8, inner bag 8.

[0026] Example 1

[0027] The basic implementation examples are as follows: Figure 1-3 As shown:

[0028] An innovative method for processing and packaging blood products, comprising:

[0029] Step 1: The blood raw material is centrifuged and separated using a processing device to separate the plasma and blood cells;

[0030] Step 2: Add low-temperature ethanol to the plasma to precipitate and extract plasma proteins;

[0031] Step 3: Select several sterile filter membranes with pore sizes corresponding to plasma proteins and blood cells, use the sterile filter membranes to perform terminal filtration of plasma proteins and blood cells, and then dispense the filtered plasma proteins and blood cells to obtain the blood products.

[0032] Example 2

[0033] The difference from the above embodiment is that: the processing device includes a housing 1, and a centrifugal assembly 2 and a water bath 8 are provided on the inner bottom wall of the housing 1. The centrifugal assembly 2 includes a base 21, and a chamber is opened in the base 21. A drive motor 23 is fixedly connected to the bottom wall of the chamber by bolts. The preferred model of the drive motor 23 is CC-M3H040-NN14. A connecting shaft 24 is coaxially welded to the output shaft of the drive motor 23. The connecting shaft 24 extends through the top wall of the chamber and out of the chamber. The connecting shaft 24 is rotatably engaged with the top wall of the chamber. A connecting seat 25 is fixedly connected to the end of the connecting shaft 24 away from the drive motor 23 by bolts. A centrifugal tube 3 is detachably connected to the connecting seat 25 by a buckle. The preferred material of the centrifugal tube 3 is polycarbonate. An inverted U-shaped outlet tube 4 is fixedly connected to the inner top wall of the housing 1 by bolts. One end of the outlet tube 4 extends... The outlet tube 4 extends into the centrifuge tube 3, and the other end of the outlet tube 4 is connected to the collection tube 7. The inner side wall of the outlet tube 4 near the collection tube 7 is fixedly connected to a first one-way valve by bolts. The first one-way valve is preferably Z-0039. The collection tube 7 is placed in the water bath 8. The outlet tube 4 has several first openings on the side wall inside the centrifuge tube 3. The first openings are arranged vertically. A first solenoid valve 41 is glued and fixed to the side wall of each first opening. The first solenoid valve 41 is preferably CPV15BP. An air bag 5 is connected to the side wall of the outlet tube 4. A second solenoid valve is glued and fixed at the connection. The second solenoid valve is preferably CPV15BP. A second opening is opened at the top of the outlet tube 4. A third solenoid valve 42 is glued and fixed to the side wall of the second opening. The third solenoid valve 42 is preferably CPV15BP.

[0034] It also includes a control system, which includes a camera and a controller. The camera is fixedly connected to the inner wall of the outer casing 1 near the centrifuge tube 3, and the controller is fixedly connected to the inner wall of the outer casing 1. The camera is used to collect image information of the centrifuged blood raw material in the centrifuge tube 3, and the controller is used to control the operation of the first solenoid valve 41 and the third solenoid valve according to the image information.

[0035] The specific implementation process is as follows: When using this device, close the first solenoid valve 41 and the second solenoid valve, place the blood raw material into the centrifuge tube 3, start the drive motor 23, and the drive motor 23 drives the centrifuge tube 3 to rotate through the connecting shaft 24 and the connecting seat 25. Due to the different densities of the components in the blood raw material, plasma, platelets, red blood cells, and white blood cells are separated into corresponding centrifuge layers under the action of centrifugal force. Then, keep the first solenoid valve 41 normally closed, open the second solenoid valve, press the air bag 5 to make the air bag 5 rebound, and at the same time close the second solenoid valve, some gas in the outlet tube 4 is discharged, causing the air pressure in the outlet tube 4 to drop. Then, start this device, and the camera continuously collects image information of each centrifuge layer in the centrifuge tube 3. The operator opens the first solenoid valve 41 located in the corresponding centrifuge layer according to the required type of blood product. Under the action of air pressure, The target liquid in the centrifugal layer enters the outlet tube 4 and then the collection tube 7. Due to the siphon principle, the target liquid will continuously flow from the outlet tube 4 into the collection tube 7. The upper centrifugal layer corresponding to the target liquid moves downward. When the upper centrifugal layer is about to approach the first solenoid valve 41, the controller controls the third solenoid valve 42 to open, allowing air to enter the inlet tube through the second opening, blocking the flow of liquid in the inlet tube. The liquid near the centrifugal tube 3 falls back into the centrifugal tube 3 under the action of gravity, so that some target liquid is retained in the centrifugal tube 3. To a certain extent, this ensures that the liquid drawn is the target liquid, thereby improving the extraction accuracy of this device. The setting of the first solenoid valve 41 and other components of this device makes it less susceptible to the influence of the number of blood cells in the blood raw material, and can achieve relatively accurate collection of different centrifugal layers to a certain extent.

[0036] Example 3

[0037] The difference from the above embodiment is that: the side wall of the outlet tube 4 is connected to the liquid storage tube 6, the liquid storage tube 6 is provided with anticoagulant, and the outer side wall of the liquid storage tube 6 is fixedly connected to the inner side wall of the outer shell 1.

[0038] The specific implementation process is as follows: When using this device, the anticoagulant required for the target liquid is added into the storage tube 6. During the movement of the target liquid in the outlet tube 4, the target liquid drives the gas at the connection between the storage tube 6 and the outlet tube 4, causing the gas pressure at that point to drop. Under the action of the gas pressure, the anticoagulant in the storage tube 6 enters the outlet tube 4 and mixes with the target liquid, thereby reducing the subsequent steps of adding anticoagulant and improving the steps for operators to process blood products.

[0039] Example 4

[0040] The difference from the above embodiment is that a second check valve is bonded and fixed at the connection between the outlet tube 4 and the liquid storage tube 6, and the preferred model of the second check valve is Z-0039.

[0041] The specific implementation process is as follows: During the use of this device, the second one-way valve can, to a certain extent, prevent the target liquid in the outlet pipe 4 from entering the storage pipe 6, thereby reducing the target liquid and causing a decrease in the production of the target blood products.

[0042] Example 5

[0043] The difference from the above embodiment is that the side walls and top walls of the chamber are both bonded and fixed with sound-absorbing panels 22 made of polyester fiber.

[0044] The specific implementation process is as follows: When using this device, the sound-absorbing plate 22 can convert the mechanical energy of the vibration generated by the drive motor 23 into its own heat energy, thereby reducing the impact of the vibration generated by the drive motor 23 on the operator's ears and improving the user experience of this device.

[0045] Example 6

[0046] The difference from the above embodiment is that: the water bath 8 is provided with an inner bag 81 and a temperature sensor. The preferred model of the temperature sensor is 718-272969-001. A semiconductor cooling chip is glued and fixed to the inner side wall of the inner bag 81. The preferred model of the semiconductor cooling chip is TEC1-04903. Both the temperature sensor and the inner bag 81 are fixedly connected to the inner side wall of the water bath 8. The temperature sensor is used to collect the temperature information of the collection tube 7. The controller controls the semiconductor cooling chip to work according to the temperature information and the image information.

[0047] The specific implementation process is as follows: When using this device, the controller determines the target liquid type corresponding to the collected centrifuged layer based on the image information, and adjusts the set temperature of the water bath in the collection tube 7 according to the target liquid type. When the temperature of the collection tube 7 exceeds the set temperature, the controller controls the semiconductor cooling chip to cool down, thereby reducing the temperature of the liquid in the water bath 8 through heat transfer, thus reducing the temperature of the collection tube 7. When the temperature of the collection tube 7 is lower than the set temperature, the controller controls the semiconductor cooling chip to heat up, thereby increasing the temperature of the liquid in the water bath 8 through heat transfer, thus increasing the temperature of the collection tube 7. This allows the temperature of the water bath 8 to change according to the different storage temperatures of different blood products, reducing the probability of deterioration during the blood product dispensing process.

[0048] The above descriptions are merely embodiments of the present invention. Commonly known structures and characteristics are not described in detail here. Those skilled in the art are aware of all common technical knowledge in the field prior to the application date or priority date, are aware of all existing technologies in that field, and have the ability to apply conventional experimental methods prior to that date. Those skilled in the art can, under the guidance of this application, improve and implement this solution in combination with their own capabilities. Some typical known structures or methods should not be obstacles for those skilled in the art to implement this application. It should be noted that those skilled in the art can make several modifications and improvements without departing from the structure of the present invention. These should also be considered within the scope of protection of the present invention, and will not affect the effectiveness of the implementation of the present invention or the practicality of the patent. The scope of protection claimed in this application should be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.

Claims

1. An innovative method for processing and packaging blood products, characterized in that: include: Step 1: The blood raw material is centrifuged and separated using a processing device to separate the plasma and blood cells; Step 2: Add low-temperature ethanol to the plasma to precipitate and extract plasma proteins; Step 3: Select several sterile filter membranes with pore sizes corresponding to plasma proteins and blood cells, use the sterile filter membranes to perform terminal filtration of plasma proteins and blood cells, and then package the filtered plasma proteins and blood cells to obtain the blood products. The processing device includes an outer shell, with a centrifuge assembly and a water bath on the bottom wall of the inner shell. The centrifuge assembly is used to centrifuge blood raw materials. Centrifuge tubes are detachably connected to the centrifuge assembly. An inverted U-shaped outlet tube is fixedly connected to the top wall of the inner shell. One end of the outlet tube extends into the centrifuge tube, and the other end of the outlet tube is connected to a collection tube. A first one-way valve is fixedly connected to the inner wall of the outlet tube near the collection tube. The collection tube is placed in the water bath. Several first openings are opened on the side wall of the outlet tube inside the centrifuge tube, and the first openings are arranged vertically. A first solenoid valve is fixedly connected to the side wall of each first opening. An air bladder is connected to the side wall of the outlet tube, and a second solenoid valve is fixedly connected to the connection. A second opening is opened at the top of the outlet tube, and a third solenoid valve is fixedly connected to the side wall of the second opening. It also includes a control system, which is electrically connected to the first solenoid valve. The control system is used to regulate the type of object entering the outlet pipe by controlling the operation of the first solenoid valve and the third solenoid valve. The control system includes a camera and a controller; The camera is used to capture images of the centrifuged blood material inside the centrifuge tubes. The controller is used to control the operation of the first and third solenoid valves based on the image information.

2. The innovative blood product processing and packaging method according to claim 1, characterized in that: The centrifuge assembly includes a base with a chamber inside. A drive motor is fixedly connected to the bottom wall of the chamber. A connecting shaft is coaxially fixedly connected to the output shaft of the drive motor. The connecting shaft extends through the top wall of the chamber and out of the chamber. The connecting shaft is rotatably engaged with the top wall of the chamber. A connecting seat is fixedly connected to the end of the connecting shaft away from the drive motor. The connecting seat is detachably connected to the centrifuge tube.

3. The innovative blood product processing and packaging method according to claim 1, characterized in that: The side wall of the outlet tube is connected to a liquid storage tube, which contains an anticoagulant.

4. The innovative blood product processing and packaging method according to claim 3, characterized in that: A second check valve is fixedly connected at the connection between the outlet tube and the reservoir tube.

5. The innovative blood product processing and packaging method according to claim 2, characterized in that: The side walls and top walls of the chamber are fixedly connected with sound-absorbing panels made of polyester fiber.

6. The innovative blood product processing and packaging method according to claim 1, characterized in that: The water bath is equipped with an inner bag and a temperature sensor. A semiconductor cooling chip is fixedly connected to the inner wall of the inner bag. The temperature sensor is used to collect temperature information from the collection tube. The controller controls the semiconductor cooling chip to work based on the temperature information and image information.

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