An insecticidal composition

Abstract

This invention provides an insecticidal composition comprising a first active ingredient of acephate or quinalphos, and a second active ingredient of bromuconazole or cyclophosphamide, wherein the weight ratio of the first active ingredient to the second active ingredient is 50:1 to 1:20. The weight percentage of the first and second active ingredients in the insecticidal composition is 1% to 90%, and the formulation of the insecticidal composition is an emulsifiable concentrate, a dispersible oil suspension, a wettable powder, or a water-dispersible granule. Compared with existing single-component formulations, the insecticidal composition of this invention has a significant synergistic effect, a significantly improved insecticidal effect, a long-lasting effect, and is safe for crops, making it worthy of widespread use in agriculture.

Description

Technical Field

[0001] This invention belongs to the field of pesticide compound technology, specifically relating to an insecticidal composition containing quinalphos. Background Technology

[0002] Organophosphate insecticides are neurotoxins that kill insects by inhibiting acetylcholinesterase, causing nerve excitation and respiratory distress. They are characterized by a broad insecticidal spectrum and low susceptibility to resistance in pests. Acetaminophen and quinalphos are both organophosphate insecticides.

[0003] Acetaminophen, or acephate, is a highly effective, low-toxicity, broad-spectrum, systemic insecticide. It is primarily used to control chewing, piercing-sucking, and boring insects, as well as mites. In China, it is mainly used to control rice leaf rollers, rice planthoppers, and cabbage caterpillars. This pesticide is characterized by its poor immediate effect but long-lasting efficacy.

[0004] Quintiofos has a broad insecticidal spectrum, with stomach poison and contact action, as well as some penetrability. It can effectively control rice stem borers, planthoppers, leafhoppers, cotton aphids, cotton bollworms, tea aphids, scale insects, etc. It has excellent efficacy, requires low application concentration, and can be widely used on a variety of crops.

[0005] Cyantraniliprole has a broad insecticidal spectrum, exhibiting both stomach poison and contact action. It can control not only lepidopteran pests but also hemiptera, coleopteran, and dipteran pests. It can be used on corn, sugarcane, cotton, grains, rice, fruit trees, and vegetables to control whiteflies, thrips, aphids, stink bugs, American serpentine leafminer, beet armyworm, rice leaf roller, rice stem borer, fruit flies, and beetles, with particularly good activity against whiteflies, leafminers, and beetles.

[0006] Cycloaniliprole, also known as chlorantraniliprole, possesses stomach poison, contact, and systemic action. It enters the insect body through ingestion and skin penetration, exhibiting rapid and highly effective insecticidal activity. It can be applied to various crops such as fruit trees, vegetables, potatoes, tea trees, soybeans, and cotton. It has a broad spectrum of control, exhibiting high biological activity against larvae and adults of Lepidoptera, Coleoptera, Thysanoptera, Diptera, and Hemiptera pests, and is unaffected by temperature. In addition to directly killing the larvae and adults of target pests, it also inhibits oviposition by adult females.

[0007] Organophosphorus insecticides have advantages such as high efficiency, broad spectrum, low price and low bioaccumulation. Currently, some organophosphorus insecticides with good bioselectivity are still widely used. However, due to long-term use, pests have developed resistance to some products. Therefore, it is particularly important to study highly efficient and low-toxic organophosphorus insecticide mixtures. Summary of the Invention

[0008] The purpose of this invention is to provide an insecticide composition that is inexpensive, has good insecticidal effect, and is less likely to cause pests to develop resistance.

[0009] To achieve the above objectives, the technical solution of the present invention is as follows:

[0010] An insecticidal composition, characterized in that it contains two active ingredients.

[0011] The primary active ingredient is acephate or quinalphos.

[0012] Second active ingredient: brofenacin or cyclofenacin

[0013] The weight ratio of the first active ingredient to the second active ingredient is 50:1 to 1:20.

[0014] In addition to the active ingredients, the pesticide compositions of the present invention, when prepared into various application formulations, also include various auxiliary ingredients that are permitted and acceptable in different pesticide formulations.

[0015] The auxiliary ingredients include, but are not limited to, emulsifiers, dispersants, wetting agents, solvents, antifreeze agents, defoamers, thickeners, disintegrants, synergists, preservatives, and fillers. These are all commonly used or permitted ingredients in pesticide formulations and are not particularly limited. The specific ingredients and dosages are determined through experiments based on the formulation requirements.

[0016] The emulsifier can be any emulsifier known in the field of pesticide formulation, and can be one or more of the following: phenylethylphenol polyoxyethylene polyoxypropylene ether, alkylaryl polyoxyethylene polyoxypropylene ether, fatty acid polyoxyethylene ether, fatty alcohol polyoxyethylene ether, glycerol laurate polyoxyethylene ether, styrene-phenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol polyoxypropylene polyoxyethylene ether, styrene-phenyl polyoxyethylene ether, alkylphenol polyoxyethylene ether, sorbitan fatty acid ester polyoxyethylene ether, fatty alcohol polyoxyethylene ether sulfonate, alkylphenol polyoxyethylene formaldehyde condensate, calcium dodecyl sulfonate, alkylphenol polyoxyethylene ether succinate monoester sulfonate, alkylphenol polyoxyethylene phosphate, and alkylphenol polyoxyethylene ether sulfonated succinate.

[0017] The dispersant can be any dispersant known in the field of pesticide formulations, and can be one or more of the following: alkylphenol polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether sulfate, phenethylphenol polyoxyethylene ether phosphate, polyoxyethylene polyoxypropylene ether block copolymer, sodium lignosulfonate, sodium methylene dinaphthalene sulfonate (dispersant NNO), calcium lignosulfonate, polycarboxylate, sodium methylene dinaphthalene sulfonate, sodium methylene dimethylnaphthalene sulfonate, polyvinylpyrrolidone, alkylamide taurate, sodium alkyl naphthalene sulfonic acid condensate, and sodium methylene dinaphthalene sulfonate formaldehyde condensate.

[0018] The wetting agent can be any wetting agent known in the field of pesticide formulation, and can be one or more of the following: sodium alkylnaphthalene sulfonate, sodium dodecyl sulfate, sodium secondary alkyl sulfate, sodium dodecylbenzene sulfonate, fatty alcohol polyethylene glycol ether sulfate, fatty alcohol polyoxyethylene ether, nonylphenol polyoxyethylene ether, sorbitan fatty acid ester polyoxyethylene ether, alkylnaphthalene formaldehyde condensate, alkylnaphthalene sulfonate, and other wetting agents.

[0019] The solvent can be any solvent known in the field of pesticide formulations, such as methanol, ethanol, N-methylpyrrolidone, N,N-dimethylformamide, N,N-dimethylacetamide, tetrahydrofurfuryl alcohol, toluene, xylene, dimethyl sulfoxide, tetrahydrofuran, cyclohexanone, n-butanol, isopropanol, and solvent oil No. 100, No. 150, No. 180, and No. 200.

[0020] The antifreeze agent can be any antifreeze agent known in the field of pesticide formulation, and can be one or more of ethylene glycol, propylene glycol, glycerol, diethylene glycol, urea, and inorganic salts.

[0021] The defoamer can be any defoamer known in the field of pesticide formulation, and can be one or more of silicone compounds, epoxidized soybean oil, emulsified silicone oil, and fatty alcohols.

[0022] The thickener can be any thickener known in the field of pesticide formulation, such as xanthan gum, polyvinyl alcohol, sodium alginate, gum arabic, or magnesium aluminum silicate.

[0023] The disintegrant can be any disintegrant known in the field of pesticide formulation, and can be one or more of ammonium sulfate, sodium carboxymethyl starch, urea, low-substituted hydroxypropyl cellulose, croscarmellose sodium, croscarmellose, sodium alginate, sodium bicarbonate, and sodium chloride.

[0024] The carrier can be any carrier known in the field of pesticide formulation, and can be one or more of glucose, silica, kaolin, diatomaceous earth, light calcium carbonate, heavy calcium carbonate, attapulgite, soluble starch, urea, maltose, sucrose, citric acid, potassium carbonate, sodium carbonate, and anhydrous sodium sulfate.

[0025] The preservative can be any preservative known in the field of pesticide formulation, such as Kathon, sodium benzoate, benzoic acid, potassium sorbate, or formaldehyde.

[0026] The deionized water mentioned is industrially produced deionized water.

[0027] The insecticidal composition of the present invention can be formulated into various agriculturally permissible formulations with the above-mentioned conventional excipients according to methods known to those skilled in the art. The formulation example used in the present invention is an emulsifiable concentrate.

[0028] The beneficial technical effects achieved by this invention are as follows:

[0029] 1. When two organophosphorus insecticides (acephate and quinalphos) are combined with bromocyanamide or cyclophosphamide respectively, they have a significant synergistic effect, which can not only improve the control efficacy, but also reduce the amount of pesticide used.

[0030] 2. Reduces the cost of drug use, resulting in lower prices;

[0031] 3. The two compounds have different mechanisms of action and can effectively delay the development of pesticide resistance in pests. Detailed Implementation

[0032] The indoor synergistic effect of the insecticidal composition of the present invention will be illustrated below by indoor toxicity testing.

[0033] 1. The combined indoor effect of acephate and bromocyanamide on rice leaf folder

[0034] First, the original drug and compound drug were prepared into six series of concentrations using organic solvents for later use. The experimental method was based on NY / T 1154.14-2008, using the rice seedling immersion and insect inoculation method. For each treatment, five rice seedlings with roots, three leaves and one heart were selected and immersed in the test drug for 10 seconds. After removing them and absorbing excess liquid on filter paper, the roots of the rice seedlings were wrapped with a damp cotton ball to keep them moist. The seedlings were then placed in prepared glass test tubes, and ten second-instar larvae of the rice leaf roller were inoculated into the test tubes. The tube openings were tied tightly with gauze. Each treatment was repeated four times. After treatment, the seedlings were placed in an observation room for cultivation (temperature 25±1℃, relative humidity about 78%, photoperiod L:D=(16:8)h).

[0035] After 72 hours, check and record the mortality of the test insects. The criterion for judging the mortality of the test insects is that if there is no reaction when the test insects are gently touched with tweezers, they are considered dead.

[0036] Mortality rate (%) = Number of dead insects / Total number of insects treated × 100

[0037] Corrected mortality rate (%) = [(treatment mortality rate - blank control mortality rate) / (1 - blank control mortality rate)] × 100

[0038] If the control mortality rate is <5%, no correction is needed; if the control mortality rate is between 5% and 20%, correction is needed; if the control mortality rate is >20%, the trial needs to be repeated.

[0039] The evaluation method for combined effects refers to the indoor bioassay test guidelines 《NY / T 1154.7-2006》. The co-toxicity coefficient (CTC) method of Sun & Johnson (1960) was used to calculate the CTC of each mixture combination. A CTC ≥ 120 indicates a synergistic effect, 80 < CTC < 120 indicates an additive effect, and a CTC ≤ 80 indicates an antagonistic effect. The formula for calculating the CTC is as follows:

[0040]

[0041] Theoretical toxicity index (TTI) = ∑(ATI of a drug × percentage of the active ingredient of the drug in the mixture)

[0042]

[0043] Table 1. Toxicity test results of acephate and cyantraniliprole mixtures against rice leaf roller.

[0044]

[0045]

[0046] Table 1 shows that the combination of acephate and cyantraniliprole at a ratio of 50:1 to 1:20 has a certain additive or synergistic effect on rice leaf roller. At a ratio of 50:1 to 1:15, it has a certain synergistic effect. When the ratio is 15:1, the co-toxicity coefficient is the largest and the synergistic effect is the most obvious.

[0047] 2. Indoor synergistic effect of quinalphos and bromocyanamide combined on rice leaf folder

[0048] The experimental method is the same as the above-mentioned method of soaking rice seedlings for insect inoculation.

[0049] Table 2. Toxicity test results of quinalphos and cyanamide mixtures against rice leaf roller.

[0050]

[0051] Table 2 shows that the combination of quinalphos and bromocyanamide in a ratio of 50:1 to 1:20 has a certain synergistic effect on rice leaf roller. When the ratio is 10:1, the co-toxicity coefficient is the largest and the synergistic effect is the most obvious.

[0052] 3. Indoor synergistic effect of acephate and cyclochlorfenapyr combined on rice stem borer

[0053] The experimental method is the same as the above-mentioned method of soaking rice seedlings for insect inoculation.

[0054] Table 3. Toxicity test results of acephate and cyclochlorfenapyr mixtures on rice stem borer.

[0055]

[0056] Table 3 shows that when acephate and cyclochlorfenapyr are combined in a ratio of 50:1 to 1:20, they have a certain synergistic or additive effect on rice stem borer. When the ratio is 50:1 to 1:15, they have a certain synergistic effect. When the ratio is 10:1, the co-toxicity coefficient is the largest and the synergistic effect is the most obvious.

[0057] 4. Indoor synergistic effect of quinalphos and cyclochlorfenapyr combined on rice stem borer

[0058] The experimental method is the same as the above-mentioned method of soaking rice seedlings for insect inoculation.

[0059] Table 4. Toxicity test results of quinalphos and cyclochlorpyrifos mixture on rice stem borer.

[0060]

[0061]

[0062] Table 4 shows that the combination of quinalphos and cyclochlorfenapyr at a ratio of 50:1 to 1:20 has a certain synergistic effect on rice stem borer. When the ratio is 8:1, the co-toxicity coefficient is the largest and the synergistic effect is the most obvious.

[0063] The invention will be further explained below with reference to formulation examples.

[0064] Formulation Example 1

[0065] The following ingredients were mixed and stirred until completely dissolved to obtain a 44% acephate-brominated cyantraniliprole emulsifiable concentrate: 40% acephate, 4% bromocyanamide, 3% N-methylpyrrolidone, 4% cyclohexanone, 5% alkylaryl polyoxyethylene polyoxypropylene ether, 3.5% calcium dodecylbenzenesulfonate, 4.5% sorbitan fatty acid ester polyoxyethylene ether, and No. 200 solvent oil to bring the total to 100%.

[0066] Formulation Example 2

[0067] The following ingredients were mixed: 45% quinalphos, 3% cyantraniliprole, 5% cyclohexanone, 6% alkylphenol formaldehyde resin polyoxyethylene ether, 5% calcium dodecylbenzene sulfonate, 3% polyoxyethylene polyoxypropylene ether block copolymer, 8% ethanol, and No. 150 solvent oil to bring the total to 100%. After mixing and stirring until completely dissolved, a 48% quinalphos·cyantraniliprole emulsifiable concentrate was prepared.

[0068] Formulation Example 3

[0069] The following ingredients were added to a total weight of 100% by weight: 16% acephate, 2% cyclophosphamide, 3% sodium lignosulfonate, 3% sodium alkyl sulfonate, Span-605%#, 3% agricultural emulsion 1601#, 3.5% polyvinyl alcohol, 3% propylene glycol, and methyl oleate. The mixture was then high-speed sheared and dispersed for 30 minutes, followed by sand milling to obtain an 18% acephate·cyclophosphamide dispersible oil suspension.

[0070] Formulation Example 4

[0071] The following ingredients were added to a 100% weight ratio: 20% quinalphos, 2% cyclochlorfenapyr, 5% sodium alkylnaphthalene sulfonate, 4% Tween-60#, 6% Span-60#, 3% agricultural emulsion 700#, 3% sodium naphthalene sulfonate, 2% ethylene glycol, 2% silica, and soybean oil. The mixture was then high-speed sheared and dispersed for 30 minutes, followed by sand milling to obtain a 22% quinalphos·cyclochlorfenapyr dispersible oil suspension.

[0072] Biological Example: Field Efficacy Trial for Controlling Rice Leaf Roller

[0073] Each community has an area of ​​30m² 2 Each treatment was replicated four times, with plots and blocks randomly arranged. A water treatment served as a blank control. Conventional spraying was used, applied during the peak hatching period of rice leaf roller eggs to the early larval stages. At the time of application, the rice plants had a few leaf tips curling and were in the tillering stage, with relatively uniform growth. The water volume was 40 L / mu, and the pesticide was applied only once throughout the experiment.

[0074] Before applying the pesticide, a baseline survey of leaf curling was conducted once, and 14 days after application, when the symptoms of damage were obvious in the blank control area, another survey was conducted.

[0075] Survey method: Five samples were taken from each plot, with five clumps at each sample point. A total of 25 clumps of rice were surveyed for the number of rolled leaves, the number of live insects inside the rolled leaves, and the total number of leaves. The rolled leaf rate, insect infestation rate, and control effect were calculated.

[0076] Method for calculating the effectiveness of prevention:

[0077] Leaf curl rate (%) = (Number of curled leaves surveyed / Total number of leaves surveyed) × 100

[0078] Infestation rate (%) = (Number of live insects inside rolled leaves / Total number of rolled leaves) × 100

[0079] Control efficacy (%) = [(Leaf curling rate in blank control area after application - Leaf curling rate in treatment area after application) / Leaf curling rate in blank control area after application] × 100

[0080] Table 5. Results of field efficacy trials of compound formulations for controlling rice leaf roller.

[0081]

[0082]

[0083] As shown in Table 5, the tested formulations (Examples 1, 2, 3, and 4) and the control formulation all exhibited good control effects against rice leaf roller. The control efficacy of the four tested formulations was higher than that of the single-agent control formulation, and field trials verified that the compositions had a certain synergistic effect.

[0084] Safety investigation: Throughout the entire experiment, no phytotoxicity was found in the rice leaves and plants caused by the application of the test drug, nor was any adverse effect on the surrounding environment found, indicating good safety.

[0085] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. An insecticidal composition, characterized in that, The insecticidal composition comprises two active ingredients: the first active ingredient is acephate and the second active ingredient is bromocyanamide, wherein the weight ratio of the first active ingredient to the second active ingredient is 50:1 to 1:

15.

2. The insecticidal composition according to claim 1, characterized in that, The weight ratio of the first active ingredient to the second active ingredient is 20:1 to 1:

1.

3. The insecticidal composition according to claim 1, characterized in that, The first and second active ingredients are present in the insecticidal composition at a weight percentage of 1% to 90%.

4. The insecticidal composition according to claim 1, characterized in that, The insecticidal composition uses the first and second active ingredients as the main active ingredients, and is formulated with pesticide adjuvants and excipients into any pesticide formulation permitted by law.

5. The insecticidal composition according to claim 4, characterized in that, The dosage form is an emulsifiable concentrate, a dispersible oil suspension, a wettable powder, or a water-dispersible granule.

6. The application of the insecticidal composition according to claim 1 in the control of rice leaf roller and rice stem borer.

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