Detection system

Abstract

This invention discloses a detection system comprising a carrier, a mounting frame, an objective lens, and a diamond. The carrier holds a liquid sample, which includes magnetic beads. The mounting frame is movable relative to the carrier. When the mounting frame is in a predetermined position, a gas chamber is formed between the objective lens, the mounting frame, and the carrier. This gas chamber contains the liquid sample and deforms the carrier when gas is introduced. The diamond is disposed outside the gas chamber and contacts the carrier to detect the liquid sample. When the mounting frame moves to the predetermined position relative to the carrier, the gas chamber is formed between the objective lens and the carrier. By introducing gas into the gas chamber, the carrier deforms, allowing the diamond to contact it. At this time, the diamond and the liquid sample on the carrier approach each other, and the NV color centers within the diamond are affected by the magnetic beads in the liquid sample, thereby achieving magnetic detection of the liquid sample.

Description

Technical Field

[0001] This invention relates to the field of magnetic detection technology, and more particularly to a biomagnetic detection system. Background Technology

[0002] Quantum diamond microscopy is a magnetic imaging device based on the NV color center and utilizing spin magnetic resonance. It employs immunomagnetic bead reactions to perform magnetic imaging of complexes containing biomarkers, measuring the magnetic or microwave field distribution around the sample. This enables quantitative and non-destructive microscopic magnetic field imaging, featuring high spatial resolution, a large field of view, a wide dynamic range of detectable magnetic fields, and fast imaging speed. How to achieve magnetic detection of liquid samples is a technical problem that urgently needs to be solved by those skilled in the art. Summary of the Invention

[0003] This invention provides a detection system.

[0004] The detection system according to embodiments of the present invention includes:

[0005] A carrier for holding a liquid sample, the liquid sample including magnetic beads;

[0006] Mounting bracket, which is movable relative to the support member;

[0007] When the mounting bracket is in a predetermined position, a gas chamber is formed between the objective lens and the carrier, the gas chamber being used to contain the liquid sample and to deform the carrier when gas is introduced;

[0008] A diamond, disposed outside the gas chamber, is used to contact the carrier and detect liquid samples.

[0009] In the detection system of this invention, when the mounting bracket moves to a predetermined position relative to the carrier, a gas chamber is formed between the objective lens and the carrier. By introducing gas into the gas chamber, the carrier can be deformed, thereby allowing the diamond to come into contact with the carrier. At this time, the diamond and the liquid sample on the carrier approach each other, and the NV color centers within the diamond can be affected by the magnetic beads within the liquid sample, thereby achieving magnetic detection of the liquid sample.

[0010] In some embodiments, a gap is formed between the mounting bracket and the objective lens, and a first seal is provided between the mounting bracket and the objective lens to seal the gap. When the mounting bracket is in a predetermined position, a gas chamber is formed between the objective lens, the first seal, the mounting bracket, and the carrier.

[0011] In some embodiments, the mounting bracket has a first opening, and when the mounting bracket is in a predetermined position, the mounting bracket is fitted against the carrier to seal the first opening.

[0012] In some embodiments, the mounting bracket has a first opening, and a slot is provided around the periphery of the first opening. A second seal is provided in the slot. When the mounting bracket is in a predetermined position, the second seal is in contact with the carrier to seal the first opening.

[0013] In some embodiments, the second seal is a star-shaped sealing ring.

[0014] In some embodiments, the carrier includes a body and a groove formed on the body, the liquid sample being contained in the groove, and when the mounting bracket is in a predetermined position, the second seal abuts against at least a portion of the groove to seal the first opening.

[0015] In some embodiments, a sample hole is formed on the bottom wall of the groove, and the liquid sample is contained in the sample hole.

[0016] In some embodiments, a positioning groove is formed circumferentially in the sample hole, and a transparent membrane substrate is provided in the positioning groove. The liquid sample is placed on the transparent membrane substrate, and a receiving hole is opened in the middle of the transparent membrane substrate. A membrane support mesh is provided in the receiving hole, and a thin film is coated on the bottom of the membrane support mesh. When gas is introduced, the membrane support mesh expands under pressure and comes into contact with the diamond.

[0017] In some embodiments, the number of sample wells is multiple.

[0018] In some embodiments, the mounting bracket has a second opening connected to a gas source to allow gas to enter the gas chamber.

[0019] In some embodiments, the detection system further includes a drive mechanism for moving the objective lens and the mounting bracket relative to the carrier.

[0020] In some embodiments, the driving mechanism includes a first driving mechanism, and the detection system further includes a beam splitter for guiding light into the objective lens and for receiving fluorescence emitted by the diamond during detection. The first driving mechanism and the mounting bracket are both mounted on the beam splitter. The first driving mechanism is connected to the objective lens and is used to drive the objective lens to move relative to the carrier.

[0021] In some embodiments, the driving mechanism includes a second driving mechanism connected to the beam splitter. The second driving mechanism is used to drive the beam splitter to move relative to the carrier, thereby causing the objective lens and the mounting bracket to move relative to the carrier, so that the mounting bracket is in a predetermined position.

[0022] In some embodiments, the detection system further includes a detector for detecting fluorescence emitted during diamond detection.

[0023] Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0024] The above and / or additional aspects and advantages of the present invention will become apparent and readily understood from the description of the embodiments taken in conjunction with the following drawings, in which:

[0025] Figure 1 This is a schematic diagram of the detection system according to one embodiment of the present invention;

[0026] Figure 2 This is a partial structural schematic diagram of the detection system according to another embodiment of the present invention;

[0027] Figure 3 yes Figure 2 A cross-sectional view of the detection system along the AA direction;

[0028] Figure 4 This is a cross-sectional view of the detection system along the AA direction when the mounting bracket is in the predetermined position;

[0029] Figure 5 yes Figure 4 Enlarged view of part A of the detection system;

[0030] Figure 6 This is a schematic diagram of the structure of a support member according to an embodiment of the present invention;

[0031] Figure 7 This is a structural schematic diagram of the support member according to another embodiment of the present invention;

[0032] Figure 8 This is a schematic diagram of the fit between the transparent film substrate and the positioning groove according to an embodiment of the present invention;

[0033] Figure 9 This is a schematic diagram of the structure of a transparent film substrate according to an embodiment of the present invention;

[0034] Figure 10 This is a schematic diagram of the process of the membrane support network expanding under pressure according to an embodiment of the present invention.

[0035] Explanation of reference numerals in the attached figures:

[0036] Detection system 100; carrier 10; mounting bracket 20; objective lens 30; gas chamber 40; diamond 50; liquid sample 200; moving module 51; gap 101; first seal 60; first opening 21; slot 22; second seal 70; body 11; groove 12; sample hole 120; positioning groove 121; transparent membrane substrate 80; receiving hole 81; membrane support mesh 82; ear groove 102; second opening 23; drive mechanism 90; first drive mechanism 91; second drive mechanism 92; spectrometer 103; detector 104. Detailed Implementation

[0037] Embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention.

[0038] In the description of this invention, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," and "counterclockwise," etc., indicating orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, are only for the convenience of describing the invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, features defined with "first" and "second" may explicitly or implicitly include one or more of the stated features. In the description of this invention, "a plurality of" means two or more, unless otherwise explicitly specified.

[0039] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection, an electrical connection, or a connection that allows for communication; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0040] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0041] The following disclosure provides many different embodiments or examples for implementing various structures of the invention. To simplify the disclosure, specific examples of components and arrangements are described below. These are merely examples and are not intended to limit the invention. Furthermore, reference numerals and / or letters may be repeated in different examples; such repetition is for simplification and clarity and does not in itself indicate a relationship between the various embodiments and / or arrangements discussed. In addition, examples of various specific processes and materials are provided in this invention, but those skilled in the art will recognize the application of other processes and / or the use of other materials.

[0042] Please see Figures 1 to 4 The detection system 100 of this invention includes a carrier 10, a mounting frame 20, an objective lens 30, and a diamond 50. The carrier 10 is used to carry a liquid sample 200, which includes magnetic beads. The mounting frame 20 is movable relative to the carrier 10. When the mounting frame 20 is in a predetermined position, a gas chamber 40 is formed between the objective lens 30, the mounting frame 20, and the carrier 10. The gas chamber 40 is used to contain the liquid sample 200 and deforms the carrier 10 when gas is introduced. The diamond 50 is disposed outside the gas chamber 40 and is used to contact the carrier 10 and detect the liquid sample 200.

[0043] In the detection system 100 of this embodiment, when the mounting bracket 20 moves to a predetermined position relative to the carrier 10, a gas chamber 40 is formed between the objective lens 30 and the carrier 10. By introducing gas into the gas chamber 40, the carrier 10 can be deformed, thereby allowing the diamond 50 to come into contact with the carrier 10. At this time, the diamond 50 and the liquid sample 200 on the carrier 10 approach each other, and the NV color centers in the diamond 50 can be affected by the magnetic beads in the liquid sample 200, thereby realizing the magnetic detection of the liquid sample 200.

[0044] Specifically, the carrier 10 is a structure used to support the sample. The carrier 10 can be a plate-like structure or a container-like structure. The shape of the mounting bracket 20 can be a regular shape, such as a prism or cylinder, or an irregular shape. The objective lens 30 is used to allow light to pass through and focus the light, thereby making the imaging of the fluorescence generated by the NV color center in the diamond 50 clearer. The light rays are shown as dashed lines in the figure, but this is only an example for ease of understanding and should not be considered as a limitation of the embodiments of the present invention. The liquid sample 200 can be a liquid containing magnetic metal elements such as iron, cobalt, and nickel, or a biological sample, and the liquid sample 200 contains magnetic beads. Magnetic beads are tiny magnetic particles that can adhere to target molecules in the liquid sample 200.

[0045] An NV center is a crystal defect present in diamond 50, consisting of a nitrogen atom and two adjacent vacancies. This defect possesses a unique electronic structure that allows it to effectively trap and store electron spin information. Furthermore, NV centers exhibit optical properties, enabling the excitation and detection of their electron spin states using light of specific wavelengths. When a magnetic bead in the liquid sample 200 approaches an NV center, the magnetic state of the bead influences the electron spin information of the NV center. By modulating the intensity and frequency of the excitation light on the NV center, the magnetic state of the bead can be read and analyzed, thereby enabling the magnetic detection of the liquid sample 200.

[0046] The mounting bracket 20 can move toward or away from the carrier 10. During the movement toward the carrier 10, the mounting bracket 20 can move to a predetermined position. The predetermined position can be the position where the mounting bracket 20 and the carrier 10 come into contact. At this time, a gas chamber 40 can be formed between the objective lens 30 and the carrier 10.

[0047] The liquid sample 200 can be located on the side of the support 10 facing the gas chamber 40. By introducing gas into the chamber, the gas pressure inside the gas chamber 40 can be increased, thereby deforming the support 10. After deformation, the support 10 can come into contact with the diamond 50 disposed outside the gas chamber 40. When the support 10 comes into contact with the diamond 50, the magnetic beads in the liquid sample 200 can interfere with the NV color centers in the diamond 50, thereby exciting the diamond 50 to emit fluorescence. The diamond 50 can be in the form of a block, a thin film, etc., and can be placed on the moving module 51. The moving module 51 can be driven by a motor drive, cylinder drive, or other means to move the diamond 50 relative to the support 10.

[0048] The gas introduced into the gas chamber 40 can be a highly stable clean gas such as nitrogen, argon, or helium. These gases do not react with the liquid sample 200 being tested and will not contaminate the liquid sample 200 being tested.

[0049] Please see Figure 3 and Figure 4 In some embodiments, a gap 101 is formed between the mounting bracket 20 and the objective lens 30, and a first seal 60 is provided between the mounting bracket 20 and the objective lens 30 to seal the gap 101. When the mounting bracket 20 is in a predetermined position, a gas chamber 40 is formed between the objective lens 30, the first seal 60, the mounting bracket 20 and the carrier 10.

[0050] Thus, by using the first sealing element 60 to seal the gap 101 between the mounting bracket 20 and the objective lens 30, the gas chamber 40 is isolated from the external environment, which can effectively reduce the interference of the external environment on the detection process and thus improve the reliability of the detection process.

[0051] Specifically, the first sealing element 60 can be a dynamic seal that seals the gap 101 between the mounting bracket 20 and the objective lens 30. The first sealing element 60 can be an elastic rubber sealing ring, a spring-type sealing ring, etc. For example, the first sealing element 60 is an elastic rubber sealing ring. The elastic rubber sealing ring can be an annular sealing ring made of rubber or similar elastic materials. The elastic rubber sealing ring has good elasticity and can adapt to the movement of the connecting part and maintain the sealing performance. The cross-section of the elastic rubber sealing ring can be annular, and the inner diameter can be slightly smaller than the outer diameter of the connecting part to ensure that it fits on the connecting part and forms an effective seal. The elastic rubber sealing ring can be installed at the gap 101 between the mounting bracket 20 and the objective lens 30 to ensure that when the mounting bracket 20 moves, the sealing ring can automatically adapt to the changing gap 101 and maintain the sealed state of the gas chamber 40.

[0052] The carrier 10 can be positioned near the bottom of the mounting bracket 20, and when the mounting bracket 20 moves downward and is in a predetermined position, the carrier 10 can contact the mounting bracket 20. The bottom of the mounting bracket 20 may have a cavity, and when the mounting bracket 20 is in the predetermined position, the gas chamber 40 formed between the objective lens 30, the first seal 60, the mounting bracket 20 and the carrier 10 may include the cavity at the bottom of the mounting bracket 20.

[0053] Please see Figure 5 In some embodiments, the mounting bracket 20 has a first opening 21, and when the mounting bracket 20 is in a predetermined position, the mounting bracket 20 fits against the carrier 10 to seal the first opening 21.

[0054] In this way, gas can contact the liquid sample 200 located on the carrier 10 through the first opening 21, and can deform the carrier 10 so that it can contact the diamond 50, thereby enabling the diamond 50 to detect the liquid sample 200. At the same time, when the mounting bracket 20 is in the predetermined position, the carrier 10 seals the first opening 21, thereby forming a gas chamber 40, and reducing the influence of the external environment on the gas chamber 40 during the detection process, thereby improving the reliability of the detection system 100.

[0055] Specifically, the size and shape of the first opening 21 need to be designed according to specific requirements. The first opening 21 allows gas to pass through, and the first opening 21 can be an opening on the end face of the mounting bracket 20 facing the carrier 10. When the mounting bracket 20 is in a predetermined position, the end face of the mounting bracket 20 facing the carrier 10 can fit against the carrier 10, thereby enabling the carrier 10 to seal the first opening 21.

[0056] Please see Figure 5 In some embodiments, the mounting bracket 20 has a first opening 21, and a groove 22 is provided around the periphery of the first opening 21. A second sealing member 70 is provided in the groove 22. When the mounting bracket 20 is in a predetermined position, the second sealing member 70 fits against the carrier member 10 to seal the first opening 21.

[0057] Thus, the second seal 70 can effectively seal the first opening 21, thereby improving the sealing performance of the gas chamber 40 and reducing the impact of the external environment on the gas chamber 40 during the detection process, thereby improving the reliability of the detection system 100.

[0058] Specifically, the slot 22 can be a structure formed by removing material from the end face of the mounting bracket 20 facing the carrier 10. The slot 22 can be set close to the edge of the first opening 21 or spaced apart from the first opening 21. The size and shape of the second seal 70 need to match the slot 22 to ensure that the second seal 70 can be fully inserted into the slot 22 and achieve an effective seal. The material and shape of the second seal 70 also need to be selected and designed according to specific requirements to ensure that it can provide good sealing performance.

[0059] The second seal 70 can be made of an elastic material, such as rubber or silicone. The second seal 70 can be a sealing ring, sealant, etc. For example, the second seal 70 is a sealing ring that engages in the slot 22. When the mounting bracket 20 is in the predetermined position, the second seal 70 can fit against the carrier 10, thereby sealing the first opening 21.

[0060] Please see Figure 5In some embodiments, the second seal 70 is a star-shaped sealing ring. Due to its shape, the star-shaped sealing ring forms a four-layer barrier structure both inside and out during installation. Therefore, the contact area between the star-shaped sealing ring and the carrier 10 is larger, thereby improving the sealing effect. This multi-layered structure effectively reduces the passage of impurities from the external environment through gaps in the sealing surface, thus mitigating the adverse effects of the external environment on the gas chamber 40.

[0061] Please see Figure 5 and Figure 6 In some embodiments, the carrier 10 includes a body 11 and a groove 12 formed on the body 11, in which the liquid sample 200 is contained. When the mounting bracket 20 is in a predetermined position, the second seal 70 is in contact with at least a portion of the groove 12 to seal the first opening 21.

[0062] During the sampling and placement process, unexpected vibrations or tilting may occur, which could cause the liquid sample 200 to detach from the support 10. Therefore, the groove 12 reduces the probability of the sample detaching from the support 10 during the testing process, preventing the liquid sample 200 from contaminating the environment and other equipment in the testing system, thereby improving the reliability of the testing process.

[0063] Specifically, the groove 12 can be formed by removing material from the body 11. For example, the body 11 can be a plate, and the groove 12 can be a blind groove formed by removing material from the plate. The end face of the groove 12 facing the second seal 70 can be provided with a frosted layer, the friction coefficient of which is greater than that of other parts of the groove 12. The frosted layer includes multiple micro-protrusions and pits, which increases the contact area between the frosted layer and the second seal 70, thereby improving the sealing effect.

[0064] Please see Figure 6 In some embodiments, a sample hole 120 is formed on the bottom wall of the groove 12, and the liquid sample 200 is contained in the sample hole 120. In this way, the sample hole 120 can be used to contain the liquid sample 200 and reduce the probability that the liquid sample 200 flows to the non-detection area during the detection process, thereby improving the reliability of the detection process.

[0065] Specifically, the sample hole 120 can be a through hole provided on the bottom wall of the groove 12 to allow light to pass through. The sample hole 120 can be of a regular shape, such as square or circular, or it can be of an irregular shape. The diameter and depth of the sample hole 120 can be set according to the volume and type of the liquid sample 200. During the detection process, the liquid sample 200 can be dripped into the sample hole 120.

[0066] Please see Figure 7 , Figure 8 , Figure 9and Figure 10 In some embodiments, a positioning groove 121 is formed circumferentially in the sample hole 120, and a transparent membrane substrate 80 is provided in the positioning groove 121. The liquid sample 200 is placed on the transparent membrane substrate 80. A receiving hole 81 is opened in the middle of the transparent membrane substrate 80, and a membrane support mesh 82 is provided in the receiving hole 81. The bottom of the membrane support mesh 82 is coated with a thin film. When gas is introduced, the membrane support mesh 82 expands under pressure and comes into contact with the diamond 50.

[0067] Thus, the positioning groove 121 facilitates the installation of the transparent film substrate 80 and defines its position, allowing the liquid sample 200 to be stably placed on it. Furthermore, when gas is introduced, the pressure within the gas chamber 40 increases, causing the membrane support mesh 82 to expand under pressure and bringing the thin film coated on its bottom into contact with the diamond 50. At this time, the liquid sample 200 is close to the diamond, allowing the magnetic beads in the liquid sample 200 to approach the NV color center of the diamond 50. By modulating the NV color center, information about the magnetic beads in the liquid sample 200 can be obtained, thus providing information about the liquid sample 200 and enabling magnetic detection of the liquid sample 200.

[0068] Specifically, the positioning groove 121 can be located on the side of the carrier 10 facing the diamond 50, so as to facilitate the contact between the film coated on the bottom of the film support mesh 82 and the diamond 50 when the transparent film substrate 80 deforms. The shape of the positioning groove 121 can be a regular shape, such as a ring or a square, or an irregular shape. The positioning groove 121 can be a blind groove, and the groove wall of the positioning groove 121 can be used to limit the transparent film substrate 80. The accommodating hole 81 in the middle of the transparent film substrate 80 means that the accommodating hole 81 is not connected to the edge of the transparent film substrate 80. The transparent film substrate 80 can be set in the positioning groove 121 by adhesive bonding. The transparent film substrate 80 can be a film with good light transmittance, non-magnetic properties, and good elasticity, such as silicon nitride film or alumina film.

[0069] The silicon nitride film substrate can be a silicon substrate, on which a film support network 82 can be formed. The film support network 82 can be used to hold the liquid sample 200. During the manufacture of the silicon nitride film, the film support network 82 can be formed by deposition through incomplete film removal or by etching the support surface.

[0070] Furthermore, the film support network 82 can be deposited separately after the silicon nitride film is manufactured, and can be composed of metals, oxides, semiconductors, or any other amorphous or crystalline material that can be deposited with appropriate spatial resolution and thickness. The film support network 82 can enhance the tensile strength of the silicon nitride film, making it less prone to damage during deformation. The film coated on the bottom of the film support network 82 can be a silicon nitride film, an alumina film, or other films with good light transmittance, non-magnetic properties, and good elasticity.

[0071] The membrane support mesh 82 reduces the rigidity of the transparent membrane substrate 80, making the membrane support mesh 82 on the transparent membrane substrate 80 easier to deform. It can be understood that when the transparent membrane substrate 80 is subjected to the gas pressure within the gas chamber 40, if the transparent membrane substrate 80 is a complete membrane without pores, it will be relatively difficult to deform due to the inherent rigidity of the transparent membrane substrate 80. However, when the receiving hole 81 is opened in the middle of the transparent membrane substrate 80 and the membrane support mesh 82 is provided, the rigidity of the membrane support mesh 82 area will decrease, making the membrane support mesh 82 more prone to deformation under external forces.

[0072] When gas is introduced, the pressure inside the gas chamber 40 increases, causing the membrane support mesh 82 to expand under pressure, thus bringing the film coated on the bottom of the membrane support mesh 82 into contact with the diamond 50. Simultaneously, the transparent membrane substrate 80 undergoes elastic deformation, and the liquid sample 200 on the transparent membrane substrate 80 collects in the region of the membrane support mesh 82 under the influence of gravity, spreading evenly on the membrane support mesh 82. Driven by the moving module 51, the diamond 50 moves towards the membrane support mesh 82 and further adheres to the film coated on the bottom of the membrane support mesh 82. At this point, the liquid sample 200 and the diamond 50 are separated only by the film coated on the bottom of the membrane support mesh 82, enabling the liquid sample 200 to be close to the NV color center of the diamond 50, thereby achieving magnetic detection of the liquid sample 200.

[0073] The transparent film substrate 80 can be bonded into the positioning groove 121. The transparent film substrate 80 can partially or completely cover the positioning groove 121 and further cover the sample hole 120. For example, a heat-sensitive adhesive can be evenly coated on the bottom of the positioning groove 121, and the transparent film substrate 80 can be clamped and bonded into the positioning groove 121 using a tooling. Then, the carrier 10 can be placed in a convection heating system (such as a heating furnace) for heating, thereby ensuring the reliability of the bonding between the transparent film substrate 80 and the positioning groove 121.

[0074] Please see Figure 8 In some embodiments, ear slots 102 may be provided at the four corners of the positioning groove 121. The ear slots 102 may be blind slots located at the four corners of the positioning groove 121, and the area of ​​the ear slots 102 may be much smaller than the area of ​​the positioning groove 121. The ear slots 102 may be used to fix the four corners of the transparent film substrate 80, specifically by filling the ear slots 102 with heat-sensitive adhesive to enhance the adhesion strength of the transparent film substrate 80.

[0075] Liquid sample 200 can be dripped onto transparent film substrate 80, and further, liquid sample 200 can be dripped onto the area of ​​transparent film substrate 80 close to sample orifice 120. When gas is introduced, the gas pressure in gas chamber 40 will increase, causing transparent film substrate 80 carrying liquid sample 200 to expand under pressure, thereby bringing transparent film substrate 80 into contact with diamond 50.

[0076] Please see Figure 6 In some embodiments, the number of sample holes 120 is multiple.

[0077] Thus, multiple sample wells 120 can increase the number of samples that can be detected simultaneously, thereby improving the detection efficiency of the detection system 100.

[0078] Specifically, the number of sample holes 120 can be two, three, four or even more, and multiple sample holes 120 can be arranged in an array on the carrier 10.

[0079] Please see Figure 4 In some embodiments, the mounting bracket 20 is formed with a second opening 23, which is connected to a gas source to allow gas to be introduced into the gas chamber 40.

[0080] In this way, the gas source can adjust the gas pressure in the gas chamber 40 by adjusting the amount of gas introduced into the gas chamber 40, and further control the deformation of the membrane support mesh 82 so that the bonding area between the film coated at the bottom of the membrane support mesh 82 and the diamond 50 can meet the field of view requirements of the objective lens 30, and meet the detection requirements of the detection system 100.

[0081] Specifically, the second opening 23 can be a structure on the mounting frame 20 that differs from the first opening 21. The second opening 23 can be formed by creating a through hole or through slot in the mounting frame 20. An air pipe connector can be installed on the mounting frame 20, which can communicate with the second opening 23, facilitating connection to a gas source. By connecting the second opening 23 to the gas source, operations such as filling, storing, and releasing gas can be performed. Specifically, gas can be introduced from the gas source into the gas chamber 40 of the mounting frame 20 through the second opening 23, or gas can be released from the gas chamber 40 into the external environment through the second opening 23.

[0082] Please see Figure 2 In some embodiments, the detection system 100 further includes a drive mechanism 90 for driving the objective lens 30 and the mounting bracket 20 to move relative to the carrier 10.

[0083] Thus, the drive mechanism 90 can drive the objective lens 30 to move, thereby automatically adjusting the focal length of the objective lens 30 to obtain better detection results. The drive mechanism 90 can also drive the mounting bracket 20 to move, thereby automatically driving the mounting bracket 20 to a predetermined position, thereby improving the detection efficiency of the detection system 100.

[0084] Specifically, the drive mechanism 90 can drive the objective lens 30 and the mounting bracket 20 using methods such as motor transmission and cylinder transmission. For example, the drive mechanism 90 can use a servo motor as the drive source, and convert the rotational motion of the servo motor into precise linear motion through a structure such as a planetary gear reducer or a lead screw and slider. The output end of the planetary gear reducer can be connected to the objective lens 30 and the mounting bracket 20, thereby realizing the movement of the objective lens 30 and the mounting bracket 20 relative to the carrier 10.

[0085] The drive mechanism 90 can drive the mount 20 and the objective lens 30 to move simultaneously or in a time-sharing manner. For example, the drive mechanism 90 may include a motor, the output shaft of which can be directly connected to the mount 20 and the objective lens 30, thereby driving the mount 20 and the objective lens 30 to move simultaneously. As another example, the drive mechanism 90 may include two motors, the output shafts of which can be connected to the mount 20 and the objective lens 30 respectively, thereby driving the mount 20 and the objective lens 30 to move in a time-sharing manner.

[0086] Please see Figure 2 , Figure 3 and Figure 4 In some embodiments, the drive mechanism 90 includes a first drive mechanism 91, and the detection system 100 also includes a beam splitter 103, which is used to guide light into the objective lens 30 and to receive the fluorescence emitted by the diamond 50 during detection. The first drive mechanism 91 and the mounting bracket 20 are both mounted on the beam splitter 103. The first drive mechanism 91 is connected to the objective lens 30 and is used to drive the objective lens 30 to move relative to the carrier 10.

[0087] Thus, the beam splitter 103 can guide light into the objective lens 30 and adjust the direction and intensity of the light, assisting in exciting the NV color centers in the diamond 50 and adjusting the intensity of the generated fluorescence, while also receiving the fluorescence emitted by the diamond 50 during detection. The first drive mechanism 91 can drive the objective lens 30 to move relative to the carrier 10, thereby automatically adjusting the focal length of the objective lens 30 to obtain better detection results. The first drive mechanism 91 and the mounting bracket 20 are both mounted on the beam splitter 103, which can optimize the space occupation of the detection system 100 and improve the space utilization rate of the components of the detection system 100.

[0088] Specifically, the first driving mechanism 91 can be the focusing stage of the piezoelectric objective lens 30. The focusing stage of the piezoelectric objective lens 30 can produce minute and precise movements, thereby driving the objective lens 30 to move relative to the carrier 10, thus achieving precise focusing of the objective lens 30. This can improve the imaging quality and accuracy of the detection system 100. The beam splitter 103 is a device for guiding light into the objective lens 30. The beam splitter 103 includes multiple lenses to ensure that the light can correctly enter the objective lens 30, so that the light can excite the NV color centers in the diamond 50 to obtain a fluorescence image.

[0089] Please see Figure 2 In some embodiments, the drive mechanism 90 includes a second drive mechanism 92 connected to the beam splitter 103. The second drive mechanism 92 is used to drive the beam splitter 103 to move relative to the carrier 10, thereby moving the objective lens 30 and the mounting bracket 20 relative to the carrier 10 so that the mounting bracket 20 is in a predetermined position.

[0090] Thus, the second driving device can drive the beam splitter 103 to move relative to the carrier 10, thereby moving the objective lens 30 and the mounting bracket 20 relative to the carrier 10. This allows the mounting bracket 20 to be positioned at a predetermined location while adjusting the focal length of the objective lens 30, thereby improving the detection efficiency of the detection system 100.

[0091] Specifically, the second drive mechanism 92 and the beam splitter 103 can be fixed and connected by connectors, such as bolts or keys. The second drive mechanism 92 can be a mechanism using a servo motor, stepper motor, or linear motor as the drive source. The speed at which the second drive mechanism 92 drives the beam splitter 103 to move relative to the support member 10 can be greater than the speed at which the first drive mechanism 91 drives the objective lens 30 to move relative to the support member 10. The second drive mechanism 92 can quickly move the mounting bracket 20 toward the support member 10, thereby enabling the mounting bracket 20 to quickly reach a predetermined position. This forms a gas chamber 40 between the objective lens 30, the first seal 60, the mounting bracket 20, and the support member 10, thereby improving the detection efficiency of the detection system 100.

[0092] Please see Figure 1 In some embodiments, the detection system 100 further includes a detector 104 for detecting the fluorescence emitted by the diamond 50 during detection.

[0093] Thus, detector 104 can detect the fluorescence emitted by diamond 50 during detection, thereby enabling the detection of the magnetic field or microwave field distribution around liquid sample 200.

[0094] Specifically, detector 104 can be a device that uses quantum diamond microscopy imaging technology to detect and image magnetic field information by utilizing the magnetic properties of quantum dots. For example, detector 104 can be a camera capable of detecting the fluorescence emitted by diamond 50 during detection. Detector 104 can collect the fluorescence signal emitted by the quantum dots when the NV color center of diamond 50 is in contact with or close to the quantum dot environment, and analyze its intensity and wavelength information to obtain detailed images or data of magnetic field changes.

[0095] Please see Figure 1 In some embodiments, the fluorescence emitted during diamond 50 detection can pass through the spectrometer 103 and be guided by the spectrometer 103 into the detector 104.

[0096] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "illustrative embodiment," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0097] Although embodiments of the invention have been shown and described, those skilled in the art will understand that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.

Claims

1. A detection system, characterized in that, The detection system includes: A carrier for holding a liquid sample, the liquid sample including magnetic beads; Mounting bracket, which is movable relative to the support member; When the mounting bracket is in a predetermined position, a gas chamber is formed between the objective lens and the carrier, the gas chamber being used to contain the liquid sample and to deform the carrier when gas is introduced; A diamond, disposed outside the gas chamber, is used to contact the carrier and detect liquid samples. A gap is formed between the mounting bracket and the objective lens, and a first sealing element is provided between the mounting bracket and the objective lens. The first sealing element is used to seal the gap. When the mounting bracket is in a predetermined position, a gas chamber is formed between the objective lens, the first sealing element, the mounting bracket and the carrier. The carrier includes a body and a groove formed on the body, wherein the liquid sample is contained in the groove; A sample hole is formed on the bottom wall of the groove, and the liquid sample is contained in the sample hole; A positioning groove is formed circumferentially in the sample hole, and a transparent membrane substrate is provided in the positioning groove. The liquid sample is placed on the transparent membrane substrate. A receiving hole is opened in the middle of the transparent membrane substrate, and a membrane support mesh is provided in the receiving hole. The bottom of the membrane support mesh is coated with a thin film. When gas is introduced, the membrane support mesh expands under pressure and comes into contact with the diamond.

2. The detection system according to claim 1, characterized in that, The mounting bracket has a first opening, and when the mounting bracket is in a predetermined position, the mounting bracket fits against the carrier to seal the first opening.

3. The detection system according to claim 1, characterized in that, The mounting bracket has a first opening, and a groove is provided around the periphery of the first opening. A second sealing element is provided in the groove. When the mounting bracket is in a predetermined position, the second sealing element fits against the carrier to seal the first opening.

4. The detection system according to claim 3, characterized in that, When the mounting bracket is in the predetermined position, the second seal is engaged with at least a portion of the groove to seal the first opening.

5. The detection system according to claim 3, characterized in that, The number of sample wells is multiple.

6. The detection system according to claim 1, characterized in that, The mounting bracket has a second opening, which is connected to a gas source to allow gas to be introduced into the gas chamber.

7. The detection system according to claim 1, characterized in that, The detection system further includes a drive mechanism for driving the objective lens and the mounting bracket to move relative to the carrier.

8. The detection system according to claim 7, characterized in that, The driving mechanism includes a first driving mechanism, and the detection system further includes a beam splitter. The beam splitter is used to guide light into the objective lens and to receive the fluorescence emitted by the diamond during detection. The first driving mechanism and the mounting bracket are both mounted on the beam splitter. The first driving mechanism is connected to the objective lens and is used to drive the objective lens to move relative to the carrier.

9. The detection system according to claim 8, characterized in that, The driving mechanism includes a second driving mechanism connected to the beam splitter. The second driving mechanism is used to drive the beam splitter to move relative to the carrier, thereby causing the objective lens and the mounting bracket to move relative to the carrier, so that the mounting bracket is in a predetermined position.

10. The detection system according to claim 1, characterized in that, The detection system also includes a detector for detecting the fluorescence emitted during diamond detection.

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