A constant temperature storage device for NK cell culture medium

Abstract

The present invention discloses a constant-temperature storage device for NK cell culture medium, which belongs to the technical field of tissue culture preservation. The present invention provides a freezer with multiple drawers that can be pulled outward, and storage boxes for storing multiple culture media are fixedly installed on the drawers. When the culture media need to be taken out, the corresponding drawer is pulled outward according to the storage position, and the inner end of the drawer box is limited to the box removal port to play a first blocking and isolation role to prevent the low-temperature environment inside the freezer from being affected by the external environment. Then, the outer ring sleeve is pushed inward to connect with the inner ring sleeve, and the outer ring sleeve and the inner ring sleeve cooperate to perform a second blocking and isolation of the culture medium at this location and the culture medium at other locations. After the material removal port is opened, the elastic band can be detached to take out the culture medium. During the entire taking process, other culture media will not be exposed to the external environment too much, and the influence of other culture media due to frequent taking out will be avoided.

Description

Technical Field

[0001] The present invention relates to the technical field of tissue culture preservation, and more particularly to a constant-temperature storage device for NK cell culture medium. Background Art

[0002] NK cells are important immune cells in the body, primarily distributed in the bone marrow, peripheral blood, liver, spleen, lungs, and lymph nodes. They are a type of lymphocyte that can non-specifically kill tumor cells and virus-infected cells without prior sensitization. Medically, NK cells can be extracted and preserved from healthy individuals. NK cell culture medium is specifically designed for the cultivation and expansion of NK cells, providing them with the nutrients and growth factors they need. Once a tumor is diagnosed, the preserved NK cells are revived and infused back into the body to kill tumor cells.

[0003] NK cell culture medium should be stored in a refrigerator or cold room below 4 degrees Celsius. Avoid exposure to high temperatures or freezing. Traditional storage devices are sealed after the culture dishes are filled and then stored in a refrigerator or cold room. When removing or placing a single culture medium, the entire end cover of the storage device must be opened, exposing the culture medium inside the storage device to the external environment, which instantly affects the internal environment of the storage device. This repeated removal and placement seriously affects the storage environment of other culture media.

[0004] To this end, we propose a constant temperature storage device for NK cell culture medium to address the above problems. Summary of the Invention

[0005] The purpose of the present invention is to solve the problem that it is difficult for existing storage devices to take out and put a single culture medium without affecting the low-temperature storage of other culture media. Compared with the existing technology, a constant-temperature storage device for NK cell culture medium is provided. By arranging multiple pull-out drawers that can be pulled outward and installed with storage boxes in the freezer, when the culture medium needs to be taken out, the corresponding pull-out drawer is pulled outward according to the storage position. The inner end of the drawer box is limited to the box removal port to play the first blocking and isolation role to prevent the low-temperature environment inside the freezer from being affected by the external environment. Then, by pushing the outer ring sleeve inward to make it dock with the inner ring sleeve, the two cooperate to perform a second blocking and isolation of the culture medium at this location and the culture medium at other positions. After the material removal port is opened, the elastic band can be removed to take out the culture medium. During the entire taking process, other culture media will not be exposed to the external environment too much.

[0006] The purpose of the present invention can be achieved through the following technical solutions:

[0007] A constant-temperature storage device for NK cell culture medium, comprising a freezer, wherein a plurality of evenly distributed partitions are fixedly disposed within the freezer, a drawer is slidably mounted on each of the partitions, an outer end wall of the freezer is provided with a box removal opening corresponding to the position of the drawer, the drawer comprising a drawer box slidably fitted on the partitions, a rotating plate rotatably mounted on the outer end wall of the drawer box for sealingly docking with the box removal opening, and a storage box is fixedly mounted on the drawer box;

[0008] The storage box includes an outer box body fixed to the drawer box, an inner box body with an upper end opening rotatably mounted on the inner bottom wall of the outer box body, a plurality of interlocking cavities for embedding culture medium are provided in a ring shape on the outer end wall of the inner box body, a plurality of elastic straps located outside the interlocking cavity are fixedly connected to the outer end wall of the inner box body, an upper cover with a bottom end face abutting against the upper end face of the inner box body is fixedly mounted on the top of the outer box body, a material taking port is provided on the end wall of the outer box body facing the rotating plate, an outer ring sleeve is slidably mounted at the material taking port, an inner ring sleeve corresponding to the outer ring sleeve is slidably mounted inside the upper cover, and grooves matching the outer ring sleeve and the inner ring sleeve are respectively provided on the end walls of the upper cover on both sides of the interlocking cavity.

[0009] Furthermore, a "cross-shaped" cavity is reserved between the two adjacent groups of partition plates on the left and right, and up and down. A refrigeration pipe installed on the inner wall of the freezer is provided in the cavity, and a refrigeration box is also installed on the outer end wall of the freezer. The refrigeration box is connected to the cavity through circulation pipes distributed up and down. The refrigeration pipe can be arranged in contact with the inner wall of the freezer, or a cooling medium can be added in the cavity, and then the refrigeration pipe is installed on the cooling medium. Multiple groups of refrigeration pipes are used to increase the constant low temperature inside the freezer, and a refrigeration box with a pair of circulation pipes is added. A refrigeration structure is also provided in the refrigeration box. When the culture medium is taken out or the temperature in the freezer fluctuates greatly, a pair of refrigeration boxes can be used to replace the air in the freezer to achieve a rapid constant temperature.

[0010] Furthermore, a pair of slide bars are fixedly connected to the bottom end of the drawer box, an inner slide groove matching the slide bar is provided on the partition plate, an outer slide groove matching the slide bar and connected to the inner slide groove is provided on the inner bottom wall of the box taking-out opening, and a buckle groove corresponding to the position of the outer slide groove is provided on the lower end wall of the rotating plate. When the pull-out drawer in the freezer needs to be pulled out, the user inserts his fingers into the buckle groove to apply outward pulling force to the rotating plate.

[0011] Furthermore, the width of the chimeric cavity is smaller than the outer diameter of the culture medium, and the culture medium is pushed into the chimeric cavity from the outside to the inside, and then the outer end wall of the culture medium is tied and limited by an elastic band.

[0012] Furthermore, the elastic band is made of elastic material, one end of the elastic band is fixedly connected to the outer end wall of the inner box body close to the mosaic cavity, the free end of the elastic band is fixedly connected to a magnetic fitting strip, and a magnetic groove matching the magnetic fitting strip is provided on the outer end wall of the inner box body close to the other side of the mosaic cavity. The magnetic fitting strip is made of magnetic material. After the culture medium is embedded in the mosaic cavity, the elastic band is pulled toward the side of the magnetic groove so that the elastic band is tightly fitted to the outer end wall of the culture medium, and the magnetic fitting strip is magnetically embedded in the magnetic groove. At this time, the elastic band plays a limiting role on the outer end wall of the culture medium, and the culture medium is stably stuck on the inner box body.

[0013] Optionally, one side end wall of the outer ring sleeve is fixedly connected to the limiting block, a slot for positioning the limiting block is provided on the outer end wall of the outer box body, the width of the inner ring sleeve is greater than the width of the outer ring sleeve, and an interlocking groove is provided on the end wall of the inner ring sleeve facing the side of the outer ring sleeve away from the limiting block, and the outer ring sleeve is embedded in the interlocking groove after the end away from the limiting block contacts the inner ring sleeve.

[0014] Optionally, the upper and lower end walls of the inner box body are fixedly connected with a rotating ring, and the inner bottom wall of the outer box body and the bottom end wall of the upper cover are both provided with an annular groove that is slidably connected with the rotating ring.

[0015] Optionally, a lower magnetic sleeve is embedded in the upper end wall of the inner box body, and an annular slide is provided on the upper end wall of the upper cover. An upper magnetic sleeve is slidably installed in the annular slide, which is magnetically attracted to the lower magnetic sleeve and corresponds to the upper and lower magnetic sleeve. When a specific culture medium needs to be taken, it is observed from the upper cover and the upper magnetic sleeve is manually rotated. Due to the magnetic attraction between the upper magnetic sleeve and the lower magnetic sleeve, the upper magnetic sleeve drives the lower magnetic sleeve to rotate together with the inner box body, thereby changing the position of the culture medium so that the culture medium to be taken out faces the side of the material taking port.

[0016] Optionally, the upper cover is made of a transparent material, and an air vent is provided on the end wall of the upper cover located on the inner side of the annular slide, which is through-and-through. The air vent is located on the inner side of the inner ring sleeve, and is used for the circulation of cold air between the inner box body and the freezer.

[0017] Compared with the prior art, the advantages of the present invention are:

[0018] (1) This solution is to set up multiple drawers that can be pulled outward in the freezer, and storage boxes for storing multiple culture media are fixedly installed on the drawers. When the culture media need to be taken out, the corresponding drawer is pulled outward according to the storage location. The inner end of the drawer box is limited to the box removal port to play the first blocking and isolation role, so as to prevent the low temperature environment inside the freezer from being affected by the external environment. Then, by pushing the outer ring sleeve inward, it passes through the inner box body and is set against the inner ring sleeve. The outer ring sleeve and the inner ring sleeve cooperate to perform a second blocking and isolation between the culture media at this location and the culture media at other locations. After the material removal port is opened, the culture media can be taken out by removing the elastic band. During the entire taking process, other culture media will not be exposed to the external environment too much, avoiding the impact on other culture media due to frequent taking.

[0019] (2) In this solution, a rotating ring is fixedly connected to the upper and lower end walls of the inner box body, and an annular groove that is slidably connected to the rotating ring is opened on the inner bottom wall of the outer box body and the bottom end wall of the upper cover. A lower magnetic sleeve is embedded in the upper end wall of the inner box body, and an annular slide corresponding to the position of the upper end wall of the upper cover is opened on the upper end wall of the upper cover. An upper magnetic sleeve that is magnetically attracted to the lower magnetic sleeve and corresponds to the upper and lower ends is slidably installed in the annular slide. The upper cover is made of transparent material. When it is necessary to take a specific culture medium, it is observed from the transparent upper cover and manually rotated. Due to the magnetic attraction between the upper magnetic sleeve and the lower magnetic sleeve, the upper magnetic sleeve drives the lower magnetic sleeve to rotate together with the inner box body, thereby changing the position of the culture medium so that the culture medium to be taken out faces the side of the material removal port. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a schematic diagram of the structure of the present invention when one of the drawers is pulled outwards. Figure 1 ;

[0021] Figure 2 Schematic diagram of the internal structure of the freezer of the present invention;

[0022] Figure 3 This is a schematic structural diagram of the freezer compartment of the present invention when the drawer and storage box are not installed;

[0023] Figure 4 This is a schematic diagram of the structure of the present invention when one of the drawers is pulled outward;

[0024] Figure 5 It is a structural schematic diagram of the pull-out drawer of the present invention;

[0025] Figure 6 The explosion at the storage box of the present invention Figure 1 ;

[0026] Figure 7 The explosion at the storage box of the present invention Figure 2 ;

[0027] Figure 8 It is a structural schematic diagram of the inner box body of the present invention;

[0028] Figure 9 This is a schematic diagram of the structure of the present invention when the outer ring sleeve is pushed inward;

[0029] Figure 10 This is a schematic structural diagram of the storage box when the culture medium is taken out of the present invention;

[0030] Figure 11 It is a schematic diagram of the overall structure of the present invention when the culture medium is taken out.

[0031] Description of the numbers in the figure:

[0032] 1. Freezer; 101. Box removal opening; 2. Partition plate; 201. Inner slide; 3. Refrigeration pipe; 4. Refrigeration box; 5. Circulation pipe; 6. Pull-out drawer; 61. Drawer box; 62. Rotating plate; 621. Buckle and pull groove; 63. Slide; 7. Storage box; 71. Outer box body; 72. Upper cover; 721. Annular slide; 73. Inner box body; 731. Rotating ring; 8. Elastic strap; 801. Magnetic fitting strip; 9. Outer ring sleeve; 901. Limit block; 10. Sealing fan plate; 11. Inner ring sleeve; 12. Lower magnetic sleeve; 13. Upper magnetic sleeve; 14. Culture medium. DETAILED DESCRIPTION

[0033] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making creative work shall fall within the scope of protection of the present invention.

[0034] Example 1:

[0035] The present invention discloses a constant temperature storage device for NK cell culture medium. Figure 1-Figure 3The refrigerator 1 has a plurality of partition plates 2 arranged in a uniform pattern, and a cavity 2 having a cross shape is reserved between two adjacent groups of partition plates 2 on the left and right sides and on the top and bottom. A refrigeration pipe 3 is arranged in the cavity, which is installed on the inner wall of the refrigerator 1, and a refrigeration box 4 is also installed on the outer end wall of the refrigerator 1. The refrigeration pipe 3 is connected to the cavity through a circulation pipe 5 distributed up and down, and the refrigeration pipe 3 is also electrically connected to the refrigeration box. The refrigeration pipe 3 can be arranged in contact with the inner wall of the refrigerator 1, or a cooling medium can be added in the cavity, and then the refrigeration pipe 3 is installed on the cooling medium. A plurality of groups of cooling pipes 3 are used to increase the constant low temperature inside the refrigerator 1, and a cooling box 4 with a pair of circulation pipes 5 is added. The cooling box 4 is also provided with a refrigeration structure. When the culture medium is taken in and out or the temperature in the refrigerator 1 fluctuates greatly, the pair of cooling boxes 4 can be used to replace the air in the refrigerator 1, and the air introduced from the outside is extracted to achieve the effect of rapid constant temperature.

[0036] See also Figure 2 and Figure 4 、 Figure 5 , a pull-out drawer 6 is slidably mounted on each partition plate 2, and a box removal opening 101 corresponding to the position of the pull-out drawer 6 is provided on the outer end wall of the freezer 1. The pull-out drawer 6 includes a drawer box 61 that is slidably fitted on the partition plate 2, and a pair of slide bars 63 are fixedly connected to the bottom end of the drawer box 61. A rotating plate 62 that is sealed and docked with the box removal opening 101 is rotatably mounted on the outer end wall of the drawer box 61, and an inner slide groove 201 that cooperates with the slide bar 63 is provided on the partition plate 2. A storage box 7 is fixedly mounted on the drawer box 61, and a slot that matches the slide bar 63 and is aligned with the inner slide groove 201 is provided on the inner bottom wall of the box removal opening 101. 1 is connected to the outer slide groove, and a buckle groove 621 corresponding to the position of the outer slide groove is opened on the lower end wall of the rotating plate 62. When the drawer 6 in the freezer 1 needs to be pulled out, the user inserts his finger into the buckle groove 621 to apply an outward pulling force to the rotating plate 62. The slide bar 63 has an L-shaped structure, and the inner slide groove 201 is deeper than the outer slide groove, which is sufficient to limit the inner end of the slide bar 63 to the end of the inner slide groove 201. When the drawer 6 is pulled outward, the inner end of the drawer box 61 is limited to the box removal opening 101. At this time, the internal environment of the freezer 1 is isolated from the outside.

[0037] See also Figure 6-Figure 8 The storage box 7 includes an outer box body 71 fixed on the drawer box 61, and an inner box body 73 with an upper end opening is rotatably installed on the inner bottom wall of the outer box body 71. A plurality of interlocking cavities for embedding the culture medium 14 are provided in a ring shape on the outer end wall of the inner box body 73. A plurality of elastic bands 8 located outside the interlocking cavity and used to limit the culture medium 14 are fixedly connected to the outer end wall of the inner box body 73. The width of the interlocking cavity is smaller than the outer diameter of the culture medium 14. The culture medium 14 is pushed into the interlocking cavity from the outside to the inside, and then the outer end wall of the culture medium 14 is limited by the elastic band 8.

[0038] An upper cover 72 is fixedly installed on the top of the outer box body 71, and its bottom end face is in contact with the upper end face of the inner box body 73. A material removal port is provided on the end wall of the outer box body 71 facing the rotating plate 62, and an outer ring sleeve 9 is slidably installed at the material removal port. An inner ring sleeve 11 corresponding to the outer ring sleeve 9 is slidably installed inside the upper cover 72, and grooves matching the outer ring sleeve 9 and the inner ring sleeve 11 are respectively provided on the end walls of the upper cover 72 on both sides of the interlocking cavity.

[0039] The elastic band 8 is made of elastic material, one end of the elastic band 8 is fixedly connected to the outer end wall of the inner box body 73 near the mosaic cavity, the free end of the elastic band 8 is fixedly connected to the magnetic fitting strip 801, and a magnetic groove matching the magnetic fitting strip 801 is provided on the outer end wall of the inner box body 73 near the other side of the mosaic cavity. The magnetic fitting strip 801 is made of magnetic material. After the culture medium 14 is embedded in the mosaic cavity, the elastic band 8 is pulled toward the side of the magnetic groove so that the elastic band 8 is tightly fitted to the outer end wall of the culture medium 14, and the magnetic fitting strip 801 is magnetically embedded in the magnetic groove, and a magnetic sheet is embedded in the magnetic groove. At this time, the elastic band 8 plays a limiting role on the outer end wall of the culture medium 14, and the culture medium 14 is stably stuck on the inner box body 73.

[0040] See also Figures 9-11 When the drawer 6 is pushed out of the freezer 1, the inner end of the drawer box 61 is limited to the box removal opening 101 to block and isolate it. Then, the rotating plate 62 is rotated outward, and the hand-held limit block 901 pushes the outer ring sleeve 9 into the outer box body 71. The outer ring sleeve 9 moves inward away from the limit block 901 at one end, penetrates the inner box body 73, and is set against the inner ring sleeve 11. After continuous pushing, the inner ring sleeve 11 moves away from the outer ring sleeve 9 at one end, penetrates the inner box body 73, and is set against the inner end wall of the outer box body 71. At this time, the limit block 901 is limited to the outer end of the material removal opening, and the outer ring sleeve 9 and the inner ring sleeve 11 cooperate to relatively isolate the culture medium 14 at this position from the culture medium 14 at other positions. Then, the elastic band 8 is detached outward, and the culture medium 14 can be easily taken out of the storage box 7.

[0041] Example 2:

[0042] Based on Example 1, this embodiment further optimizes the rotation operation of the inner box body 73 to solve the problem of taking and placing multiple culture media 14 corresponding to one material taking port, as follows:

[0043] See also Figure 6-Figure 7The upper and lower end walls of the inner box body 73 are fixedly connected to the rotating ring 731, and the inner bottom wall of the outer box body 71 and the bottom end wall of the upper cover 72 are provided with annular grooves that are slidably connected to the rotating ring 731. The upper end wall of the inner box body 73 is embedded with a lower magnetic sleeve 12, and the upper end wall of the upper cover 72 is provided with an annular slide 721. The annular slide 721 is slidably installed with an upper magnetic sleeve 13 that is magnetically attracted to the lower magnetic sleeve 12 and corresponds to the upper and lower parts. The upper cover 72 is made of transparent material. When it is necessary to take a specific culture medium 14, it can be observed from the transparent upper cover 72 and manually rotated. Due to the magnetic attraction of the upper magnetic sleeve 13 and the lower magnetic sleeve 12, the upper magnetic sleeve 13 drives the lower magnetic sleeve 12 together with the inner box body 73 to rotate, thereby changing the position of the culture medium 14 so that the culture medium 14 to be taken out faces the side of the material taking port.

[0044] An air vent is provided on the end wall of the upper cover 72 located inside the annular slide 721 and extending vertically therethrough. The air vent is located inside the inner ring sleeve 11 and is used for the circulation of cold air between the inner box body 73 and the freezer 1 .

[0045] It should be added here that one side end wall of the outer ring sleeve 9 is fixedly connected to the limiting block 901, and a slot for positioning the limiting block 901 is provided on the outer end wall of the outer box body 71. The width of the inner ring sleeve 11 is greater than the width of the outer ring sleeve 9. An interlocking groove is provided on the end wall of the inner ring sleeve 11 facing the outer ring sleeve 9 away from the limiting block 901. The outer ring sleeve 9 is embedded in the interlocking groove after the end away from the limiting block 901 contacts the inner ring sleeve 11.

[0046] The inner ring sleeve 11 is slidably installed in the inner box body 73. In the initial state, the inner box body 73 is located inside the upper cover 72, exposing the through groove corresponding to the inner ring sleeve 11. At this time, the interior of the inner box body 73 is connected to the inner wall of the outer box body 71. The space between the inner box body 73 and the outer box body 71 is in a connected space, and the culture medium 14 is in the same constant low-temperature environment inside and outside. However, according to actual needs, the inner ring sleeve 11 can also be fixedly installed on the inner wall of the outer box body 71, and the inner end of the inner ring sleeve 11 extends to the inside of the inner box body 73. At this time, the outer box body 71 and the inner box body 73 are connected through the through groove corresponding to the outer ring sleeve 9. The inner ring sleeve 11 also serves as a spacing between multiple culture media 14. Although the connectivity is reduced, the isolation effect is better.

[0047] In combination with Example 1 and Example 2, a method for using a constant temperature storage device for NK cell culture medium includes the following steps:

[0048] Step 1: When storing the culture medium 14 to be stored, first pull out the multiple drawers 6 to open the storage box 7, and take the inner box body 73 out of the outer box body 71. Then, insert the culture medium 14 into the fitting cavity of the inner box body 73 in turn, and use the elastic band 8 to bind and limit the culture medium 14. After the culture medium 14 is placed, install the inner box body 73 into the outer box body 71, and then fix the upper cover 72 on the top of the outer box body 71. The upper magnetic sleeve 13 and the lower magnetic sleeve 12 are positioned correspondingly.

[0049] This process can also be done without opening the storage box 7, and the culture medium 14 can be placed into the chimeric cavity one by one directly through the material taking port on the outer box body 71, according to actual needs;

[0050] Step 2: After the workpieces are placed, the multiple drawers 6 are retracted into the freezer 1, the rotating plate 62 is sealed at the box removal opening 101, and the multiple groups of refrigeration pipes 3 are used to control the temperature inside the freezer 1;

[0051] Step 3: When it is necessary to take out the culture medium 14, the corresponding drawer 6 is pulled outward according to the storage position, and the inner end of the drawer box 61 is limited to the box taking port 101 to play a blocking and isolating role, and then the rotating plate 62 is rotated outward, and the hand-held limit block 901 pushes the outer ring sleeve 9 into the outer box body 71. The end of the outer ring sleeve 9 away from the limit block 901 moves inward to penetrate the inner box body 73 and abuts against the inner ring sleeve 11. After continuous pushing, the inner ring sleeve 11 away from the end of the outer ring sleeve 9 penetrates the inner box body 73 and abuts against the inner end wall of the outer box body 71. At this time, the limit block 901 is limited to the outer end of the material taking port, and the outer ring sleeve 9 and the inner ring sleeve 11 cooperate to relatively isolate the culture medium 14 at this location from the culture medium 14 at other locations;

[0052] Step 4: Take out the magnetic bonding strip 801 again, and the elastic band 8 returns to its contracted state. The elastic band 8 loses its limiting effect on the culture medium 14. At this time, the culture medium 14 can be easily taken out. After the material is taken out, pull and rotate the outer ring sleeve 9 in the opposite direction. The outer ring sleeve 9 closes the material taking port, and the inner ring sleeve 11 is tightly connected to the outer ring sleeve 9. It moves with the outer ring sleeve 9 to the end wall on the other side of the inner box body 73 and is limited and separated from the outer ring sleeve 9. Flip it upward and engage the rotating plate 62. Finally, push the drawer 6 inward. The rotating plate 62 is re-sealed at the box taking port 101 to complete a single take-in and put, avoiding affecting other culture media 14 due to frequent taking.

[0053] In summary, the present invention provides a plurality of outwardly pull-out drawers 6 in the freezer 1. Storage boxes 7 for storing a plurality of culture media 14 are fixedly mounted on the drawers 6. When the culture media 14 need to be taken out, the corresponding drawer 6 is pulled outward according to the storage location. The inner end of the drawer box 61 is restricted at the box removal opening 101, which acts as a first blocking and isolation function, thereby preventing the low-temperature environment inside the freezer 1 from being affected by the external environment.

[0054] Then, by pushing the outer ring sleeve 9 inward, the material removal port is opened. After continuous pushing, the outer ring sleeve 9 moves inward to penetrate the inner box body 73 and is positioned against the inner ring sleeve 11. The outer ring sleeve 9 and the inner ring sleeve 11 cooperate to perform a second sealing and isolation of the culture medium 14 at this location and the culture medium 14 at other locations. After the material removal port is opened, the culture medium 14 can be taken out by removing the elastic band 8. During the entire taking process, other culture media will not be exposed to the external environment too much, and the impact on other culture media 14 due to frequent taking will be avoided.

[0055] The above are only preferred specific implementation methods of the present invention; however, the protection scope of the present invention is not limited thereto; any technician familiar with the technical field within the technical scope disclosed by the present invention; any equivalent replacement or change based on the technical solution and improved conception of the present invention shall be covered within the protection scope of the present invention.

Claims

1. A constant temperature storage device for NK cell culture medium, comprising a freezer (1), characterized in that: A plurality of evenly distributed partitions (2) are fixed inside the freezer (1), a drawer (6) is slidably mounted on each of the partitions (2), an outer end wall of the freezer (1) is provided with a box removal opening (101) corresponding to the position of the drawer (6), the drawer (6) comprises a drawer box (61) slidably fitted on the partition (2), a rotating plate (62) is rotatably mounted on the outer end wall of the drawer box (61) and is sealed and docked with the box removal opening (101), and a storage box (7) is fixedly mounted on the drawer box (61); The storage box (7) includes an outer box body (71) fixed on the drawer box (61), an inner box body (73) with an upper end opening is rotatably mounted on the inner bottom wall of the outer box body (71), a plurality of interlocking cavities for embedding the culture medium (14) are annularly provided on the outer end wall of the inner box body (73), a plurality of elastic straps (8) located outside the interlocking cavity are fixedly connected to the outer end wall of the inner box body (73), an upper cover (72) whose bottom end face contacts the upper end face of the inner box body (73) is fixedly mounted on the top of the outer box body (71), a material taking port is provided on the end wall of the outer box body (71) facing the rotating plate (62), an outer ring sleeve (9) is slidably mounted on the material taking port, an inner ring sleeve (11) corresponding to the outer ring sleeve (9) is slidably mounted inside the upper cover (72), and grooves matching the outer ring sleeve (9) and the inner ring sleeve (11) are respectively provided on the end walls of the upper cover (72) on both sides of the interlocking cavity; The end wall of one side of the outer ring sleeve (9) is fixedly connected to the limit block (901), and a slot for positioning the limit block (901) is provided on the outer end wall of the outer box body (71). The width of the inner ring sleeve (11) is greater than the width of the outer ring sleeve (9), and an engaging notch is provided on the end wall of the inner ring sleeve (11) facing the outer ring sleeve (9) away from the limit block (901). The end of the outer ring sleeve (9) away from the limit block (901) contacts the inner ring sleeve (11) and is then embedded in the engaging notch. The upper and lower end walls of the inner box body (73) are both fixedly connected to the rotating ring (731), and the inner bottom wall of the outer box body (71) and the bottom end wall of the upper cover (72) are both provided with an annular groove that is slidably connected to the rotating ring (731).

2. A constant temperature storage device for NK cell culture medium according to claim 1, characterized in that: A cavity distributed in a "cross" shape is reserved between two groups of adjacent partition plates (2) on the left and right sides and on the top and bottom sides. A refrigeration pipe (3) installed on the inner wall of the freezer (1) is provided in the cavity, and a refrigeration box (4) is also installed on the outer end wall of the freezer (1). The refrigeration box (4) is connected to the cavity through a circulation pipe (5) distributed on the top and bottom sides.

3. The constant temperature storage device for NK cell culture medium according to claim 1, characterized in that: A pair of slide bars (63) are fixedly connected to the bottom end of the drawer box (61); an inner slide groove (201) matching the slide bar (63) is provided on the partition plate (2); an outer slide groove matching the slide bar (63) and connected to the inner slide groove (201) is provided on the inner bottom wall of the box removal opening (101); and a buckle groove (621) corresponding to the position of the outer slide groove is provided on the lower end wall of the rotating plate (62).

4. The constant temperature storage device for NK cell culture medium according to claim 1, characterized in that: The width of the chimeric cavity is smaller than the outer diameter of the culture medium (14).

5. The constant temperature storage device for NK cell culture medium according to claim 1, characterized in that: The elastic band (8) is made of elastic material, one end of the elastic band (8) is fixedly connected to the outer end wall of the inner box body (73) close to the chimeric cavity, the free end of the elastic band (8) is fixedly connected to a magnetic fitting strip (801), and a magnetic groove matching the magnetic fitting strip (801) is provided on the outer end wall of the inner box body (73) on the other side close to the chimeric cavity, and the magnetic fitting strip (801) is made of magnetic material.

6. The constant temperature storage device for NK cell culture medium according to claim 1, characterized in that: A lower magnetic sliding sleeve (12) is embedded in the upper end wall of the inner box body (73), and an annular slideway (721) is provided on the upper end wall of the upper cover (72). An upper magnetic sliding sleeve (13) is slidably installed in the annular slideway (721) and is magnetically attracted to the lower magnetic sliding sleeve (12) and corresponds to the upper and lower magnetic sliding sleeves (13).

7. The constant temperature storage device for NK cell culture medium according to claim 6, characterized in that: The upper cover (72) is made of a transparent material, and an air hole is provided on the end wall of the upper cover (72) located on the inner side of the annular slideway (721).

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