Application of paenibacillus chibensis space mutant in sewage treatment

Abstract

The application discloses application of a Paenibacillus kribbensis space mutant in sewage treatment, and provides a new application of a Paenibacillus kribbensis space mutant PS04-17 strain. Research shows that the PS04-17 strain can be used for sewage treatment, and has a good ammonia-nitrogen degradation effect on aquaculture wastewater treatment, can effectively reduce the ammonia-nitrogen content in the wastewater, has a total nitrogen degradation rate of 88.9%, and has an ammonia-nitrogen degradation rate of 96.7%. The PS04-17 strain has a good biological flocculation effect when used for sewage treatment, can remove suspended solids in water, improve water quality, and can improve the biodegradation capacity of the wastewater. The PS04-17 strain can effectively treat sewage, remove ammonia-nitrogen in the sewage, reduce the use of chemical agents, reduce secondary pollution of the water body, effectively repair the ecological system of the water body, and protect the ecological environment, and therefore has important significance for effective treatment of sewage.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of microbial degradation. More specifically, it relates to the application of a space mutant of Paenibacillus chibensis in sewage treatment. BACKGROUND

[0002] With the growth of global population and the acceleration of industrialization process, sewage treatment has become an important field of environmental protection. Traditional sewage treatment methods face many challenges and problems, including high energy consumption, low treatment efficiency, and secondary pollution caused by discharged sludge. Traditional sewage treatment methods mainly include physical, chemical and biological treatment processes. Physical treatment usually uses mesh or sieve to remove large solid substances, but it is powerless for tiny particles or dissolved pollutants. Chemical treatment precipitates suspended solids and oxidizes organic matter by adding chemical agents, however, this method not only needs a large amount of agent input, but also produces a large amount of wastewater. Biological treatment is a more environmentally friendly method, which uses microorganisms to degrade organic matter into harmless substances, but the biological treatment process is sensitive to temperature, pH value, oxygen content and other conditions, and is easily affected by external environmental changes, and the existing microbial species is limited. In addition, traditional sewage treatment methods also have problems such as long treatment period, high energy consumption, complex operation, large equipment footprint, etc. For some remote areas or developing countries, traditional treatment processes are not suitable or difficult to implement.

[0003] Due to the fact that aquaculture wastewater contains a large amount of pollutants such as ammonia nitrogen compounds, phosphorus, organic matter, etc.; contains high COD, high ammonia nitrogen, high suspended solids; and contains a large amount of pathogenic microorganisms and parasitic eggs; it can make the water body smell, produce toxic gases such as methane and hydrogen sulfide, and cause a large number of aquatic organisms to die; a large amount of suspended solids in wastewater can make the water body turbid, reduce the photosynthesis of algae in water, limit the normal activities of aquatic organisms, and cause the gradual death of aquatic organisms sensitive to organic pollution; some bacteria, viruses, etc. in urine and feces can flow with water and cause the spread of some epidemics, etc.

[0004] The common method for treating aquaculture wastewater is: first, pretreatment, solid-liquid separation technology and coagulation sedimentation method or coagulation air flotation method are used to remove suspended solids, color and part of organic matter, then biochemical treatment is carried out, and through the way of anaerobic-anoxic-aerobic, removal of organic matter, ammonia nitrogen pollutants and phosphorus pollutants is realized, finally, through advanced treatment process (chemical precipitation method, oxidation ditch, disinfection), standard discharge or reuse is realized. Among them, in the biochemical treatment process, the aerobic treatment refers to a process for treating aquaculture wastewater by using aerobic microorganisms, common microorganisms include hydrolysis and fermentation bacteria, hydrogen-producing and acetic acid-producing bacteria, methanogenic bacteria, and homoacetogenic bacteria population, and the nitrogen and phosphorus pollutants in water are metabolically decomposed by microorganisms, so that the purpose of purifying water quality is achieved. At present, there are still few microbial degradation bacteria for aquaculture wastewater pollution, and there is no report on the use of Paenibacillus kribbensis for sewage treatment. Therefore, it is urgent to provide more microbial degradation bacteria which can be applied to sewage treatment in an efficient, energy-saving and environmentally friendly manner, to reduce the pollution of aquaculture wastewater, and effectively remove harmful substances and reduce pollutant emissions, so as to achieve the goal of sustainable development and environmental protection. SUMMARY

[0005] The technical problem to be solved by the present application is to overcome the shortcomings of the existing sewage treatment strains, and to provide the application of Paenibacillus kribbensis space mutant in sewage treatment.

[0006] The purpose of the present application is to provide a new application of Paenibacillus kribbensis space mutant in sewage treatment.

[0007] Another purpose of the present application is to provide a sewage treatment method.

[0008] Still another purpose of the present application is to provide a method for flocculating particulate matter in water.

[0009] The above purposes of the present application are achieved by the following technical solutions:

[0010] The application provides a new application of a space mutation strain of Paenibacillus kribbensis, namely, a PS04-17 strain, in sewage treatment, and the strain has been preserved in the Guangdong Microbial Culture Collection Center on March 10, 2022, with a preservation number of GDMCC NO:62233 and a preservation address of 100, Xianlie Middle Road, Guangzhou. Research shows that the fermentation liquor of the PS04-17 strain can effectively reduce the total nitrogen value and ammonia nitrogen content in polluted water, the total nitrogen degradation rate reaches 88.9%, and the ammonia nitrogen degradation rate reaches 96.7%; the PS04-17 strain has good bioflocculation effect in sewage treatment, can remove the particulate suspended matter in water, and can be used for improving water quality and improving the biodegradation capacity of wastewater. The PS04-17 strain can not only degrade ammonia nitrogen, but also effectively reduce the chemical oxygen demand (COD content) in wastewater, remove ammonia nitrogen in water, reduce the pollution degree of water by reducing substances, effectively treat sewage, reduce the use of chemical agents, reduce secondary pollution of water, repair the ecological system of water, and protect the ecological environment.

[0011] Therefore, the application provides the application of the space mutation strain of Paenibacillus kribbensis, namely, the PS04-17 strain, or a bacterial liquor thereof in sewage treatment.

[0012] Further, the sewage treatment refers to removing ammonia nitrogen in wastewater, reducing the total nitrogen content and COD content.

[0013] The application provides the application of the space mutation strain of Paenibacillus kribbensis, namely, the PS04-17 strain, in nitrogen removal.

[0014] The application provides the application of the space mutation strain of Paenibacillus kribbensis, namely, the PS04-17 strain, or a bacterial liquor thereof in degrading ammonia nitrogen, reducing the total nitrogen content, or in preparing an ammonia nitrogen degradation preparation.

[0015] The application provides the application of the space mutation strain of Paenibacillus kribbensis, namely, the PS04-17 strain, or a bacterial liquor thereof in preparing a bioflocculant.

[0016] The application provides a sewage treatment method, which uses the space mutation strain of Paenibacillus kribbensis, namely, the PS04-17 strain, or a bacterial liquor thereof to treat sewage.

[0017] Preferably, the bacterial solution is a fermentation solution, and the concentration is not less than 1x10 9 cfu / mL.

[0018] Preferably, the sewage is aquaculture wastewater.

[0019] The application also provides a method for flocculating particulate matter in water, which comprises adding a fermentation solution of a space mutation strain of Paenibacillus kribbensis, △PS04-17, to the water.

[0020] Preferably, the bacterial solution is a fermentation solution, and the concentration is not less than 1x10 9 cfu / mL.

[0021] The application has the following beneficial effects:

[0022] The application provides a new application of a space mutation strain of Paenibacillus kribbensis, △PS04-17, in sewage treatment. Research shows that the space mutation strain of Paenibacillus kribbensis, △PS04-17, has the effect of degrading ammonia nitrogen and can be used for sewage treatment, which can effectively reduce the total nitrogen value and ammonia nitrogen content in polluted water, and the degradation rate of total nitrogen can reach 88.9%, and the degradation rate of ammonia nitrogen can reach 96.7%. The application of the △PS04-17 strain in sewage treatment can be used for treating sewage with high ammonia nitrogen, especially aquaculture wastewater, reducing the dependence on the external environment, and effectively removing harmful substances and reducing pollutant emissions, so as to achieve the goal of sustainable development and environmental protection.

[0023] At the same time, the application of the space mutation strain of Paenibacillus kribbensis, △PS04-17, in sewage treatment also has good bioflocculation effect, which can remove particulate suspended matter in water, improve water quality, and improve the biodegradation capacity of wastewater. The use of the △PS04-17 strain not only can degrade nitrogen-containing components in sewage and effectively treat sewage problems, but also can reduce the use of chemical agents and reduce secondary pollution of water bodies, thereby protecting the ecological environment. BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1 The comparison of the ammonia nitrogen content in the sewage of the control group and the treatment group; CK is the control group, and HTB is the treatment group.

[0025] Figure 2 The comparison of the total nitrogen content in the sewage of the control group and the treatment group; CK is the control group, and HTB is the treatment group.

[0026] Figure 3 The comparison of the flocculation effect of the sewage of the control group and the treatment group; CK is the control group, and HTB is the treatment group. DETAILED DESCRIPTION

[0027] The present application will be further described in conjunction with the accompanying drawings and specific examples, but the examples do not limit the present application in any form. Unless otherwise specified, the reagents, methods and equipment used in the present application are conventional reagents, methods and equipment in the technical field.

[0028] Unless otherwise specified, the reagents and materials used in the following examples are commercially available.

[0029] Example 1 Preparation of Paenibacillus kribbensis fermentation broth

[0030] The Paenibacillus kribbensis space mutation strain △PS04-17 used in this example is the previous research result of the research group of the present applicant, and the △PS04-17 strain has been preserved in the Guangdong Microbial Culture Collection Center on March 17, 2022, with the preservation number of GDMCC NO:62233, the preservation address of Guangzhou City, and is disclosed in the prior art: CN 114480222A (patent publication number).

[0031] Preparation of fermentation broth: inoculate △PS04-17 bacterial liquid into modified Czapek liquid medium (sodium nitrate 1.5 g / L, potassium phosphate dibasic 1 g / L, magnesium sulfate 0.5 g / L, potassium chloride 0.5 g / L, ferrous sulfate 0.01 g / L, sucrose 30 g / L) with an inoculation amount of 1%, and ferment at 25-30℃, 150 rpm for 7 days to prepare a fermentation broth containing Paenibacillus kribbensis space mutation strain △PS04-17, and the concentration of the fermentation broth is 1×10 9 cfu / mL.

[0032] Experimental Example 2 Application in sewage treatment

[0033] The fermentation broth of Paenibacillus kribbensis space mutation strain △PS04-17 prepared in Example 1 was used for testing, and the test was carried out in Guangzhou Shangyi Ecological Co., Ltd. The sewage used in the test sample was pig farm breeding wastewater. The pig farm breeding wastewater was diluted, and after dilution 5 times, the total nitrogen content in the breeding wastewater was detected to be 900 mg / L, and the ammonia nitrogen content was 750 mg / L.

[0034] The pig farm breeding wastewater was diluted and treated, and divided into two groups: a control group (CK) and a treatment group (HTB). The control group was treated blankly, and the treatment group was added with 10 mL of △PS04-17 fermentation broth for treatment for 4 days. The other treatment conditions of the two groups were kept consistent. And the total nitrogen value and ammonia nitrogen value in the water body were detected by gas phase molecular absorption spectroscopy.

[0035] The total nitrogen values in the water body at different times after statistical processing are shown in Table 1. The results show that the total nitrogen value in the aquaculture wastewater is significantly reduced by 50% on the second day after the HTB group is treated with the fermentation broth of △PS04-17. The total nitrogen value in the aquaculture wastewater continues to decrease with time, and the effect is better than that of the CK group. The total nitrogen value is reduced to 100 mg / L on the fourth day, which is decreased by 88.9% compared with the initial value. Figure 1 ).

[0036] Table 1 Total nitrogen values in water body under different treatments

[0037]

[0038] Note: The values in the table are in mg / L.

[0039] Further statistical processing of the ammonia nitrogen content in the water body at different times shows that the ammonia nitrogen value in the aquaculture wastewater is significantly reduced by 46.7% on the third day after the HTB group is treated with the fermentation broth of △PS04-17. Further observation shows that the total nitrogen value in the treatment group is reduced to 25 mg / L on the fourth day, which is decreased by 96.7% compared with the initial value. Figure 2 ).

[0040] Table 2 Ammonia nitrogen content in water body under different treatments

[0041]

[0042] Note: The values in the table are in mg / L.

[0043] Example 3 Flocculation effect in aquaculture wastewater

[0044] Based on the research of Example 2, this example studies the treatment of aquaculture wastewater with the fermentation broth of the space mutation strain △PS04-17 of the Paenibacillus chibensis. The results show that adding the fermentation broth of the △PS04-17 strain in the pig farm aquaculture wastewater has a good biological flocculation effect. Figure 3 The particles suspended in the water interact with each other to form larger clumps, forming flocculation, which facilitates subsequent separation and removal. Further preparation of the space mutation strain △PS04-17 of the Paenibacillus chibensis as a biological flocculant can help remove these suspended solids and improve water quality.

[0045] Further, through chemical detection of the aquaculture wastewater, it is found that the fermentation broth of △PS04-17 can effectively reduce the chemical oxygen demand (COD content) in the wastewater. While removing ammonia nitrogen in the water body, it also reduces the degree of pollution by reducing substances in the water body; at the same time, it improves the biodegradability of the wastewater.

[0046] The above research shows that the application of the space mutation mutant strain PS04-17 of Paenibacillus campinasensis provided by the application can be used for sewage treatment, especially sewage of high ammonia nitrogen type, can effectively reduce the total nitrogen value and ammonia nitrogen content in the polluted water body, the total nitrogen degradation rate can reach 88.9%, and the ammonia nitrogen degradation rate can reach 96.7%; also has good bioflocculation effect, can be used for removing suspended solids in the water body, improving water quality, and improving the biodegradation capacity of wastewater. Using the strain PS04-17 can not only degrade the nitrogen-containing components in the sewage, effectively treat the sewage problem, but also can reduce the use of chemical agents, reduce the secondary pollution of the water body, and protect the ecological environment.

[0047] The above examples are the preferred embodiments of the application, but the embodiments of the application are not limited to the above examples, and any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the application should be equivalent replacement methods, and are all included in the protection scope of the application.

Claims

1. Cribbenoid Bacillus ( Paenibacillus kribbensis The application of the space-induced mutant strain △PS04-17 or its bacterial culture in wastewater treatment is characterized by, The wastewater treatment refers to the removal of ammonia nitrogen from wastewater and the reduction of total nitrogen and COD levels; the *Cribenzia* species mentioned are... Paenibacillus kribbensis The space-induced mutant strain △PS04-17 was deposited at the Guangdong Provincial Center for Microbial Culture Collection on March 17, 2022, with accession number GDMCCNO: 62233.

2. Cribbenoid Bacillus ( Paenibacillus kribbensis Application of the space-induced mutant strain △PS04-17 as a denitrifying bacterium; the Cribbenoid Bacillus ( Paenibacillus kribbensis The space-induced mutant strain △PS04-17 was deposited at the Guangdong Provincial Center for Microbial Culture Collection on March 17, 2022, with accession number GDMCC NO: 62233.

3. Cribbenoid Bacillus ( Paenibacillus kribbensis The application of the space-induced mutant strain △PS04-17 or its bacterial culture in degrading ammonia nitrogen, reducing total nitrogen content, or in the preparation of ammonia nitrogen degradation agents; the Cribbenoid Bacillus ( Paenibacillus kribbensis The space-induced mutant strain △PS04-17 was deposited at the Guangdong Provincial Center for Microbial Culture Collection on March 17, 2022, with accession number GDMCC NO: 62233.

4. Cribbenoid Bacillus ( Paenibacillus kribbensis The application of the space-induced mutant strain △PS04-17 or its bacterial culture in the preparation of bioflocculants; the Cribben-like Bacillus ( Paenibacillus kribbensis The space-induced mutant strain △PS04-17 was deposited at the Guangdong Provincial Center for Microbial Culture Collection on March 17, 2022, with accession number GDMCC NO: 62233.

5. A wastewater treatment method, characterized in that, Using Cribbella ( Paenibacillus kribbensis The space-induced mutant strain △PS04-17 or its bacterial culture is used to treat wastewater; the Cribben-like Bacillus ( Paenibacillus kribbensis The space-induced mutant strain △PS04-17 was deposited at the Guangdong Provincial Center for Microbial Culture Collection on March 17, 2022, with accession number GDMCC NO: 62233.

6. The method according to claim 5, characterized in that, The bacterial solution is a fermentation broth with a concentration of not less than 1×10⁻⁶. 9 cfu / mL.

7. The application according to claim 1, or the method according to claim 5, characterized in that, The sewage is aquaculture wastewater.

8. A method for flocculating particulate matter in water, characterized in that, Bacillus cristatus (Cribbella) Paenibacillus kribbensis The fermentation broth of the space-induced mutant strain △PS04-17 was added to the water; the Cribben-like Bacillus ( Paenibacillus kribbensis The space-induced mutant strain △PS04-17 was deposited at the Guangdong Provincial Center for Microbial Culture Collection on March 17, 2022, with accession number GDMCC NO: 62233.

Images