A composition for regulating sebum secretion, and a method of preparing and using the same

Abstract

The application discloses a kind of compositions for regulating oil secretion and preparation method and application thereof;The composition includes the following mass percentage of ingredients: azelate di-glycine potassium 1-5%;APN activity inhibitor 1-5%;Plankton extract 1-4%;Nicotinamide 1-2%;The composition of the application inhibits the activity of type I 5alpha-reductase, cyclooxygenase-2 and aminopeptidase N in sebaceous gland cells, improves keratinization of hair follicle orifice, dredges pores, and regulates water and oil balance, and inhibits sebaceous gland cell activity through multi-pathway synergy, jointly regulates the secretion of oil;It fundamentally inhibits the synthesis and secretion of oil in sebaceous gland cells.The composition of the application is suitable for application in various cosmetics for regulating the secretion of oil on scalp or skin, and has good industrial application prospect.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and particularly relates to a composition for regulating oil secretion, a preparation method and application thereof. BACKGROUND

[0002] The human scalp is an important part of the skin, with all the structures and functions of the skin in general, but as a unique ecosystem, it also has characteristics such as dense hair, more sweat glands and sebaceous glands, and higher relative humidity.

[0003] The scalp, as the soil on which the hair lives, the balance of its ecological environment is the foundation of the healthy growth of the hair. As part of the balance of the scalp ecosystem, appropriate oil secretion can keep the scalp and hair moisturized, but when the sebaceous glands are affected by endocrine or external stimuli and other factors, excessive oil secretion will cause the scalp to become oily, the reproduction rate of lipophilic fungi (such as Pityrosporum ovale) will increase, and then lead to the breaking of the scalp microbial flora homeostasis and the change of the physiological environment, and easily induce seborrheic dermatitis and even hair loss. In order to protect the ecological balance of the scalp and maintain the freshness and health of the scalp and hair, regulating oil secretion has gradually become the focus of researchers.

[0004] The existing scalp oil control mainly uses physical cleaning and adsorption, shrinking pores, inhibiting 5α-reductase activity, etc., and cannot effectively regulate oil secretion on the scalp, because:

[0005] (1) Physical cleaning and adsorption can only temporarily remove the oil that has been secreted on the surface of the scalp, and cannot change the situation of excessive secretion of sebum. Excessive cleaning may even lead to "compensatory" secretion of sebum, prompting the sebaceous glands to secrete more oil to repair the sebum film, thereby exacerbating the oiliness, and also easily damaging the skin barrier; physical adsorption may cause blockage of the pores due to the presence of micron-sized porous particles on the surface of the scalp, affecting the smooth discharge of the secreted oil, and may cause the occurrence of acne.

[0006] (2) Controlling oil secretion by merely shrinking pores can only reduce the discharge of oil on the surface of the scalp, and the apparent oiliness of the scalp and hair is reduced, but the oil secreted by the sebaceous glands will accumulate in the duct connecting the sebaceous glands and the hair follicle orifice, which will induce the occurrence of comedones, closed pores and acne.

[0007] (3) Inhibiting 5α-reductase activity is to prevent testosterone entering sebaceous glands from being converted into 5α-dihydrotestosterone (DHT) by type I 5α-reductase, thereby inhibiting the proliferation and differentiation of sebaceous gland cells and the secretion of oil. Since 5α-reductase inhibitors are androgen-dependent, for men with excessive oil secretion on the scalp, inhibiting the activity of 5α-reductase in sebaceous gland cells can significantly improve oil secretion, but for normal women with low serum testosterone levels (relative to men), inhibiting the activity of 5α-reductase in sebaceous gland cells obviously cannot effectively improve oil secretion; K. Seiffert found in the research reported in Hormone and Metabolic Research 2007; 39 (2): 141-148 that inhibiting 5α-reductase activity alone is insufficient to reduce overall sebaceous gland cell activity. Therefore, controlling oil secretion by inhibiting 5α-reductase activity alone has certain limitations, and other oil control pathways need to be found to work synergistically with 5α-reductase inhibition to jointly regulate oil secretion on the scalp.

[0008] Current products or technologies for regulating oil secretion can only target a certain factor that leads to excessive oil secretion, or, starting from the mechanism of sebaceous gland secretion, only select a certain pathway that affects sebaceous gland secretion to regulate oil secretion, without systematically and comprehensively analyzing multiple different pathways that affect sebaceous gland secretion. Therefore, the use effect is limited to a certain extent, and the problem of excessive oil secretion cannot be effectively and completely solved. SUMMARY

[0009] In view of the deficiencies and limitations of the existing technologies for regulating oil secretion on the scalp, in order to effectively inhibit oil secretion on the scalp, the purpose of the present application is to provide a composition for regulating oil secretion on the scalp through multiple pathways, which inhibits the activity of type I 5α-reductase, cyclooxygenase (COX-2), and aminopeptidase N (APN) in sebaceous gland cells, improves keratinization of hair follicle orifices, unblocks pores, and regulates water and oil balance, thereby synergistically inhibiting the activity of sebaceous gland cells and jointly regulating the secretion of oil on the scalp. The composition can also be used in skin care products, and also has an oil control effect.

[0010] The technical solution adopted by the present application is as follows:

[0011] In a first aspect of the present application, a composition is provided, which comprises the following ingredients in the following mass percentages: potassium azelate 1-5%; an APN activity inhibitor 1-5%; a plankton extract 1-4%; and nicotinamide 1-2%.

[0012] Preferably, the APN activity inhibitor is one or both of a Photinia serratifolia extract and a Camellia sinensis leaf extract.

[0013] The present application explores a method for inhibiting excessive secretion of oil by analyzing multiple pathways affecting the sebaceous gland to secrete oil and factors causing excessive secretion of oil, including reducing the secretion of oil from the sebaceous gland by inhibiting the activity of oil control targets such as type I 5α-reductase, cyclooxygenase (COX-2) and aminopeptidase N (APN); removing the secreted oil by appropriate physical cleaning and adsorption; removing the secreted oil by exfoliating the keratin of the hair follicle orifice and unblocking the pores; and improving the skin's external oiliness and internal dryness by hydrating and moisturizing the skin and regulating the water-oil balance.

[0014] Cyclooxygenase COX-2 is an inducible enzyme that is not expressed in most normal tissues, but the expression of COX-2 in normal hair follicle sebaceous gland units can promote the differentiation of hair follicle stem cells into sebaceous gland cells, leading to sebaceous gland hyperplasia and increased sebaceous secretion; plankton extract can down-regulate the gene expression of COX-2 involved in sebaceous secretion and reduce the synthesis of prostaglandin (E2), thereby inhibiting the differentiation of hair follicle stem cells into sebaceous gland cells and affecting the secretion of oil.

[0015] Aminopeptidase N (APN) is an exopeptidase that can hydrolyze neutral amino acids at the N-terminus of oligopeptides and is involved in cell proliferation and differentiation. By inhibiting the activity of APN, the proliferation of sebaceous gland cells is inhibited, terminal differentiation is enhanced, and neutral lipid synthesis is inhibited, thereby achieving the purpose of reducing sebaceous secretion. Flavonoids, as an APN inhibitor, can ultimately reduce oil secretion by inhibiting the synthesis of triglycerides. The extract of Photinia serratifolia and the extract of Ilex asprella are rich in proanthocyanidins, which are a type of bioflavonoids with a special molecular structure and have a strong inhibitory effect on APN activity.

[0016] 5α-reductase converts androgen-testosterone into dihydrotestosterone (DHT) in sebaceous gland cells through an endocrine pathway, thereby affecting DNA transcription, promoting sebaceous gland cell proliferation, differentiation and secretion of sebum. There are many components that inhibit 5α-reductase activity, including zinc salt, capryloyl glycine, sarcosine and azelaic acid and its derivatives. The present application selects azelaic acid derivatives with relatively high inhibition efficiency as inhibitors affecting the pathway. Azelaic acid has been confirmed by many aspects as a strong 5α-reductase inhibitor in human skin. In the study of Stamatiadis in British Journal of Dermatology (1988; 119: 627-632), the inhibition rate of 3mmol / l azelaic acid on 5a-reductase activity was 98%; under the same conditions, the concentration of zinc sulfate required to achieve 98% inhibition of 5a-reductase activity was 15mmol / l, which indicates that the inhibition efficiency of azelaic acid on 5a-reductase activity is greater than that of zinc sulfate. Due to the poor solubility, high melting point and large amount of azelaic acid, as well as poor compatibility, its practical application is greatly limited. The present application uses azelaic acid derivative-potassium azelate, which has good water solubility and is not easy to discolor, and has excellent safety and stability compared with azelaic acid.

[0017] The raw materials that can successfully discharge the secreted sebum by softening the keratinization of the hair follicle orifice, exfoliating the keratin and unblocking the pores include fruit acids, salicylic acid and niacinamide. However, the application of salicylic acid and fruit acids is limited to a certain extent due to their irritancy to the scalp. Niacinamide is an amide compound of nicotinic acid and also a water-soluble vitamin. It promotes the exfoliation of sebum in the ducts connected to the sebaceous glands and the skin surface, accelerates the exfoliated sebum in the ducts to the skin surface, reduces the storage of sebum in the ducts, and ultimately leads to the reduction of sebum on the scalp surface. In addition, niacinamide can also reduce the production of fatty acids and triglycerides in sebum to a certain extent, thereby achieving the effect of controlling sebum secretion. Therefore, niacinamide can replace salicylic acid and fruit acids to gently exfoliate the keratin of the hair follicle orifice and unblock the sebum secretion pipeline.

[0018] Preferably, the mass ratio of the photinia extract and the black tea leaf extract is 1:0.5-2.

[0019] Preferably, the composition further comprises a humectant.

[0020] Preferably, the humectant comprises at least one of 1,3-propanediol, propylene glycol, glycerol, butylene glycol, panthenol, sodium hyaluronate and its derivatives, trehalose, and xylitol glucoside.

[0021] Preferably, the mass percentage of the humectant is 0.1-1.5%.

[0022] Preferably, the remaining amount in the composition is water.

[0023] In a second aspect of the present application, a method for preparing the composition of the first aspect of the present application is provided, comprising the following steps: mixing the humectant, the potassium azelate, the APN activity inhibitor, the plankton extract and the nicotinamide uniformly.

[0024] In a second aspect of the present application, the composition of the first aspect of the present application is used in the preparation of an oil-control cosmetic.

[0025] Preferably, the oil-control cosmetic comprises a care product or a cleansing product.

[0026] Preferably, the care product comprises one or more of a serum, a hair conditioner, a hair mask, a skin cream, a skin jelly, a lotion, a facial mask, a toner.

[0027] Preferably, the cleansing product comprises one or more of a shampoo, a scrub, a body wash, a facial cleanser.

[0028] Preferably, the mass percentage of the composition of the first aspect of the present application in the oil-control cosmetic is 5-15%.

[0029] Preferably, the oil-control cosmetic further comprises an excipient.

[0030] In a third aspect of the present application, an oil-control cosmetic is provided, comprising the composition of the first aspect of the present application.

[0031] Preferably, the mass percentage of the composition of the first aspect of the present application in the oil-control cosmetic is 5-15%.

[0032] Preferably, the oil-control cosmetic further comprises an excipient.

[0033] Preferably, the excipient comprises at least one of a thickening agent, a pH adjuster, a skin conditioning agent, a penetration enhancer, a preservative.

[0034] Preferably, the thickening agent comprises at least one of carbomer, acrylates / C10-30 alkyl acrylate crosspolymer, xanthan gum, cellulose ether.

[0035] Preferably, the pH adjuster comprises at least one of aminomethyl propanol, sodium hydroxide, potassium hydroxide, triethanolamine, arginine, citric acid, sodium citrate, lactic acid, sodium lactate.

[0036] Preferably, the skin conditioning agent comprises at least one of dipotassium glycyrrhizinate, trehalose, allantoin.

[0037] Preferably, the penetration enhancer comprises at least one of ethanol, propylene glycol, menthol.

[0038] Preferably, the preservative comprises at least one of phenoxyethanol, ethylhexylglycerin, caprylyl hydroxamic acid, p-hydroxyacetophenone, caprylyl glycol, 1,2-hexanediol.

[0039] Preferably, the thickening agent has a mass percentage of 0.1-1%.

[0040] Preferably, the pH adjuster has a mass percentage of 0.05-0.15%.

[0041] Preferably, the skin conditioning agent has a mass percentage of 0.5-1%.

[0042] Preferably, the penetration enhancer has a mass percentage of 8-15%.

[0043] Preferably, the preservative has a mass percentage of 0.5-1.5%.

[0044] The beneficial effects of the present application are:

[0045] The present application provides a multi-pathway composition for regulating sebum secretion on the scalp, which inhibits the activity of type I 5α-reductase, cyclooxygenase (COX)-2, and aminopeptidase N (APN) in sebocytes, improves keratosis of the hair follicle orifice, unblocks pores, and regulates water-oil balance, thereby synergistically inhibiting the activity of sebocytes and jointly regulating sebum secretion on the scalp. Compared with existing oil control technologies or products, the multi-pathway composition for regulating sebum secretion on the scalp of the present application has excellent effects of inhibiting sebum secretion, can inhibit the synthesis and secretion of oil in sebocytes from different enzyme pathways that affect the secretion of oil by sebaceous glands, promote the discharge of secreted oil from the duct to the surface of the scalp by improving keratosis of the hair follicle orifice and unblocking pores, and finally restore the water-oil imbalance on the surface of the scalp caused by excessive secretion of oil by moisturizing and hydrating effects of the moisturizing agent. The present application is suitable for application in various cosmetics for regulating sebum secretion on the scalp or skin, and has good prospects for industrial application. DETAILED DESCRIPTION

[0046] The concept and technical effects of the present application will be described below in conjunction with examples for a clear and complete understanding of the purpose, features, and effects of the present application. Obviously, the described examples are only some of the examples of the present application, but not all the examples. Based on the examples of the present application, other examples obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0047] Example 1

[0048] A multi-channel regulating oil secretion composition, consisting of the following components (in percentage by weight): potassium azelate diglycine 3%, black tea leaf extract 2%, plankton extract 2.5%, nicotinamide 2%, panthenol 1%, and the balance deionized water.

[0049] The preparation steps are as follows:

[0050] (1) According to the above ratio, deionized water and panthenol are added to the container, heated to 80-85℃, continuously stirred to mix evenly, and kept warm for 10-20 min;

[0051] (2) The temperature is lowered to about 40-45℃, and potassium azelate diglycine, black tea leaf extract, plankton extract, and nicotinamide are added, continuously stirred and mixed evenly, and the temperature is continuously lowered to about 37℃. After sampling and testing, the multi-channel regulating oil secretion composition is obtained.

[0052] Example 2

[0053] A multi-channel regulating oil secretion composition, consisting of the following components (in percentage by weight): potassium azelate diglycine 3%, black tea leaf extract 2%, plankton extract 2.5%, nicotinamide 2%, panthenol 1%, and the balance deionized water.

[0054] The preparation steps are as follows:

[0055] (1) According to the above ratio, deionized water and panthenol are added to the container, heated to 80-85℃, continuously stirred to mix evenly, and kept warm for 10-20 min;

[0056] (2) The temperature is lowered to about 40-45℃, and potassium azelate diglycine, black tea leaf extract, plankton extract, and nicotinamide are added, continuously stirred and mixed evenly, and the temperature is continuously lowered to about 37℃. After sampling and testing, the multi-channel regulating oil secretion composition is obtained.

[0057] Example 3

[0058] A multi-channel regulating oil secretion composition, consisting of the following components (in percentage by weight): potassium azelate diglycine 3%, black tea leaf extract 2%, plankton extract 2.5%, nicotinamide 2%, panthenol 1%, and the balance deionized water.

[0059] The preparation steps are as follows:

[0060] (1) According to the above ratio, deionized water and panthenol are added to the container, heated to 80-85℃, continuously stirred to mix evenly, and kept warm for 10-20 min;

[0061] (2) cooling to about 40-45°C, adding potassium azelate, extract of black tea leaf, plankton extract, nicotinamide, continuing to stir and mix evenly, continuing to cool to about 37°C, after sampling and testing are qualified, the multi-channel regulation of oil secretion composition is obtained.

[0062] Example 4

[0063] A multi-channel regulation of oil secretion composition, consisting of the following components (in percentage by weight): potassium azelate 1.5%, extract of Photinia frasery, extract of black tea leaf 3%, plankton extract 1%, nicotinamide 1%, 1,3-propanediol 2%, and the balance of deionized water.

[0064] The preparation steps are as follows:

[0065] (1) according to the above ratio, deionized water and 1,3-propanediol are added to the container, heated to 80-85°C, continuously stirred to mix evenly, and kept warm for 10-20 min;

[0066] (2) cooling to about 40-45°C, adding potassium azelate, extract of Photinia frasery, extract of black tea leaf, plankton extract, nicotinamide, continuing to stir and mix evenly, continuing to cool to about 37°C, after sampling and testing are qualified, the multi-channel regulation of oil secretion composition is obtained.

[0067] Example 5

[0068] A multi-channel regulation of oil secretion composition, consisting of the following components (in percentage by weight): potassium azelate 5%, extract of Photinia frasery 1%, extract of black tea leaf 1%, plankton extract 1.5%, nicotinamide 1%, panthenol 1.5%, and the balance of deionized water.

[0069] The preparation steps are as follows:

[0070] (1) according to the above ratio, deionized water and panthenol are added to the container, heated to 80-85°C, continuously stirred to mix evenly, and kept warm for 10-20 min;

[0071] (2) cooling to about 40-45°C, adding potassium azelate, extract of Photinia frasery, extract of black tea leaf, plankton extract, nicotinamide, continuing to stir and mix evenly, continuing to cool to about 37°C, after sampling and testing are qualified, the multi-channel regulation of oil secretion composition is obtained.

[0072] Test 1: Human sebaceous gland cell SZ95 lipid secretion inhibition test

[0073] The above prepared multi-channel regulating oil secretion composition of Example 1-5 was subjected to human sebocyte SZ95 oil secretion inhibition effect test.

[0074] 1. SZ95 cell culture

[0075] After the cryopreserved SZ95 cells were recovered, they were cultured in DMEM high-sugar medium containing 10% fetal bovine serum, 1 x 105 U / L penicillin, and 100 mg / L streptomycin at 37°C in a cell incubator with 5% CO2. When the cells adhered and grew, and the confluence exceeded 80%, they were digested and subcultured with trypsin containing 0.25% EDTA, and the logarithmic growth phase cells were used for experiments.

[0076] 2. MTT detection experiment based on human sebocyte SZ95 cells

[0077] (1) Cell inoculation: Logarithmic growth phase SZ95 cells were inoculated in a 96-well plate at a density of 1 x 10 4 cells / well, and cultured in an incubator (37°C, 5% CO2) for 24 hours.

[0078] (2) Test grouping: The test was set up with a zero setting group, a solvent control group, and a sample group. In the sample group, four concentration gradients (0.002%, 0.02%, 0.2%, and 2%) were set up for each sample, and three repeated wells were set up for each concentration gradient.

[0079] (3) Drug administration: When the cell plating rate in the 96-well plate reached 40%-60%, drug administration was performed. The solvent control group was added with 200 μL of culture solution per well; the sample group was added with 200 μL of culture solution containing the corresponding concentration of the example composition per well; the zero setting group was not inoculated with cells, but was added with 200 μL of culture solution only. After drug administration was completed, the 96-well plate was placed in an incubator (37°C, 5% CO2) and cultured for 24 hours.

[0080] (4) Detection: After the cells were incubated and cultured for 24 hours, the supernatant was removed, 20 μL of MTT solution (0.5 mg / mL) was added for treatment, and incubation was performed at 37°C in the dark for 4 hours. After incubation was completed, the supernatant was removed, 150 μL of DMSO was added to each well, and the plate was placed on a shaker for 10-15 minutes to completely dissolve the formazan crystals. The absorbance (OD) was measured at 570 nm using an enzyme-linked immunoassay instrument, and the relative survival rate of the cells was calculated according to the following formula.

[0081] Relative survival rate of cells (%) = (OD of sample well - OD of zero setting well) / (OD of solvent control well - OD of zero setting well) * 100%.

[0082] Table 1 is the relative survival rate of cells of the oil control composition (Example 1-5)

[0083]

[0084]

[0085] According to the MTT detection results in Table 1, when the dilution concentration of Examples 1-5 is 0.002-0.02%, the relative survival rate of cells is 86-98% compared with the solvent control group, and there is no cytotoxicity, so it can be determined that 0.01% and 0.02% are used as two dilution concentrations of Examples 1-5 for testing in subsequent experiments.

[0086] 3. Nile red staining method for detecting neutral lipids in SZ95 cells

[0087] The logarithmically growing SZ95 cells were digested and counted, and then transferred to each sample well of a 96-well plate at a concentration of 1×10 4 The supernatant was aspirated after 24 h of incubation, and then washed once with PBS. Then 100 μL of the corresponding solution was added according to Table 2, and each group was incubated at 37°C, 5% CO2, and saturated humidity for 48 h. After 48 h of incubation, the DMEM complete medium was discarded, and the cells were carefully washed twice with PBS, and then 10 μg / mL Nile red was added, and the cells were incubated at 37°C in the dark for 15 min. The released fluorescence was detected using a multifunctional enzyme label instrument with fluorescence function at an excitation wavelength of 485 nm and an absorption wavelength of 565 nm, and 3 replicate wells were used for each group. The inhibition rate of the oil control composition on the lipid synthesis of SZ95 cells was calculated according to the following formula.

[0088] Relative content of intracellular neutral lipids (%) = OD of sample group / OD of blank control group*100%;

[0089] Lipid inhibition rate (%) = (OD of blank control group-OD of sample group) / (OD of blank control group)*100%;

[0090] Table 2 Experimental scheme for inhibiting the secretion of oil by human sebaceous gland cells SZ95 cells

[0091]

[0092]

[0093] Oleic acid and linoleic acid can significantly promote the synthesis of neutral lipids in SZ95 cells as stimulators of the secretion of oil by sebaceous gland cells, and the present application simulates an important factor leading to excessive secretion of sebum by human skin.

[0094] Table 3 Effect of different concentrations of the sample to be tested on the level of intracellular neutral lipids

[0095]

[0096] From Table 3, it can be seen that the relative content of neutral lipids in the cells of the positive control group decreased significantly compared with the blank control group, indicating that the positive control was effective in this test. The relative content of neutral lipids in the cells of Examples 1-5 also decreased to varying degrees compared with the blank control group. For example, the relative content of neutral lipids in the cells of Example 1 decreased by 26.7% and 31.3% at dilution concentrations of 0.01% and 0.02%, respectively, compared with the blank control group, and the lipid inhibition rate was higher than that of other examples, indicating that the potassium azelate dipeptide, black tea leaf extract or lindera umbellata thunb extract, plankton extract and nicotinamide in the composition had a synergistic effect, and the content of one or two or three components had an impact on the inhibition of oil secretion. Therefore, the components in the compositions of Examples 1-5 had a synergistic effect when they were compounded in a specific ratio, which could significantly inhibit the secretion of oil by sebaceous gland cells.

[0097] Test 2: Red blood cell hemolysis test

[0098] The eye irritation degree of the above-mentioned composition was evaluated by detecting the influence of the composition on the release of hemoglobin and the denaturation of hemoglobin, and the composition with relatively small irritation was screened.

[0099] 500 uL of 2% red blood cell suspension was mixed with 500 uL of sample solution with mass fraction of 20%, 10%, 2%, 1%, 0.5%, 0.3%, 0.2% and 0.1% (diluted with PBS) in equal volume, and 2% red blood cell suspension was mixed with physiological saline in equal volume as the negative group, and 2% red blood cell suspension was mixed with 1% SDS solution in equal volume as the positive group, and three groups of parallel were set in each group. After being placed in a 37°C biochemical incubator for 3h, 10 uL of supernatant and 90 uL of physiological saline were taken and placed in a 96-well plate for determination of absorbance values at 410, 540 and 575 nm.

[0100]

[0101]

[0102] The regression equation was determined according to the hemolysis rate, the sample concentration HC50(mg / L) when 50% of red blood cells were hemolyzed by the test sample was calculated, and the protein denaturation index DI when the sample concentration was 1% was calculated. The L / D value of the sample was calculated from the HC50 value and the DI value: L / D = HC50 / DI. According to the red blood cell hemolysis test grading standard of European Center for Validation of Alternative Methods (ECVAM), the irritation degree of the test sample was graded. As shown in Table 4 below.

[0103] Table 4 ECVAM cosmetic red blood cell hemolysis test irritation grading standard

[0104]

[0105] Table 5. Results of hemolysis test of red blood cells of compositions of Examples 1-5

[0106]

[0107] From the results of the test in Table 5, it can be seen that the compositions of Examples 1-5 are all non-irritating to the eyes.

[0108] Comparative Example 1

[0109] A multi-channel sebum secretion regulating composition, which is different from the components of Example 1 in that it does not contain potassium azelate diglycine, and deionized water is the balance, and the other components and proportions are unchanged, and the preparation method is consistent with Example 1.

[0110] Comparative Example 2

[0111] A multi-channel sebum secretion regulating composition, which is different from the components of Example 1 in that it does not contain black tea leaf extract, and deionized water is the balance, and the other components and proportions are unchanged, and the preparation method is consistent with Example 1.

[0112] Comparative Example 3

[0113] A multi-channel sebum secretion regulating composition, which is different from the components of Example 1 in that it does not contain plankton extract, and deionized water is the balance, and the other components and proportions are unchanged, and the preparation method is consistent with Example 1.

[0114] Comparative Example 4

[0115] A multi-channel sebum secretion regulating composition, which is different from the components of Example 1 in that it does not contain nicotinamide, and deionized water is the balance, and the other components and proportions are unchanged, and the preparation method is consistent with Example 1.

[0116] Comparative Example 5

[0117] A multi-channel sebum secretion regulating composition, which is different from the components of Example 1 in that it does not contain potassium azelate diglycine and black tea leaf extract, and deionized water is the balance, and the other components and proportions are unchanged, and the preparation method is consistent with Example 1.

[0118] Comparative Example 6

[0119] A multi-channel sebum secretion regulating composition, which is different from the components of Example 1 in that it does not contain potassium azelate diglycine and plankton extract, and deionized water is the balance, and the other components and proportions are unchanged, and the preparation method is consistent with Example 1.

[0120] Comparative Example 7

[0121] A multi-channel oil secretion regulating composition which is different from the components of Example 1 in that it does not contain potassium azelate diglycine and nicotinamide, and the balance is deionized water, other components and proportions remain unchanged, and the preparation method is consistent with Example 1.

[0122] Comparative Example 8

[0123] A multi-channel oil secretion regulating composition which is different from the components of Example 1 in that it does not contain black tea leaf extract and plankton extract, and the balance is deionized water, other components and proportions remain unchanged, and the preparation method is consistent with Example 1.

[0124] Comparative Example 9

[0125] A multi-channel oil secretion regulating composition which is different from the components of Example 1 in that it does not contain black tea leaf extract and nicotinamide, and the balance is deionized water, other components and proportions remain unchanged, and the preparation method is consistent with Example 1.

[0126] Comparative Example 10

[0127] A multi-channel oil secretion regulating composition which is different from the components of Example 1 in that it does not contain plankton extract and nicotinamide, and the balance is deionized water, other components and proportions remain unchanged, and the preparation method is consistent with Example 1.

[0128] Comparative Example 11

[0129] A multi-channel oil secretion regulating composition which is different from the components of Example 1 in that it does not contain potassium azelate diglycine, black tea leaf extract and plankton extract, and the balance is deionized water, other components and proportions remain unchanged, and the preparation method is consistent with Example 1.

[0130] Comparative Example 12

[0131] A multi-channel oil secretion regulating composition which is different from the components of Example 1 in that it does not contain black tea leaf extract, plankton extract and nicotinamide, and the balance is deionized water, other components and proportions remain unchanged, and the preparation method is consistent with Example 1.

[0132] Comparative Example 13

[0133] A multi-channel oil secretion regulating composition which is different from the components of Example 1 in that it does not contain potassium azelate diglycine, black tea leaf extract and nicotinamide, and the balance is deionized water, other components and proportions remain unchanged, and the preparation method is consistent with Example 1.

[0134] Comparative Example 14

[0135] A multi-channel composition for regulating sebum secretion, which is different from the components of Example 1 in that it does not contain potassium azelate diglycine, plankton extract and nicotinamide, and the balance is deionized water, and the other components and proportions remain unchanged, and the preparation method is consistent with Example 1.

[0136] Comparative Example 15

[0137] A multi-channel composition for regulating sebum secretion, which is different from the components of Example 1 in that it does not contain potassium azelate diglycine, plankton extract and nicotinamide, and the balance is deionized water, and the other components and proportions remain unchanged, and the preparation method is consistent with Example 1.

[0138] Application Example 1

[0139] The application example provides a serum for regulating sebum secretion of the scalp, and the formula is shown in Table 6:

[0140] Table 6 Formulation of a serum for regulating sebum secretion of the scalp

[0141]

[0142] The preparation method is as follows:

[0143] 1. Put deionized water in the emulsifying kettle, and slowly put carbomer into the emulsifying kettle under homogenization, and start heating;

[0144] 2. Stop heating when the temperature rises to about 80-85°C, and homogenize and stir until the carbomer is completely swollen in water, and then start cooling;

[0145] 3. When the temperature is reduced to 45°C, add the B phase raw material, neutralize and thicken, stir uniformly, then add the C phase raw material, continue to stir for 10-20 min, and continue to cool;

[0146] 4. When the temperature is reduced to about 37°C, add the D and E phase raw materials, stir for 10-20 min, and ensure that the components are mixed uniformly;

[0147] 5. After sampling and testing, a serum for regulating sebum secretion of the scalp containing the composition of the application is obtained.

[0148] Comparative Application Examples 1-15

[0149] The comparative application example provides 15 serums, and the formula is different from that of application example 1 in that the composition of example 1 is replaced by the composition of comparative examples 1-15 respectively, and the preparation method is consistent with that of application example 1.

[0150] Test 3: Test of sebum content on the scalp

[0151] (1) Test subjects: oily scalp, aged 18-30, with the informed consent of the test subjects, after the test subjects were washed with the same shampoo, they were left for 20 minutes, and the scalp oil content was measured as the initial value, denoted as T0, using a skin oil tester.

[0152] (2) Test environment: test environment temperature 20-22℃, humidity 40-60%;

[0153] (3) Test instrument: skin oil tester Sebumeter SM815 (Courage & Khazaka, Germany);

[0154] (4) Test method: 34 test subjects were selected, and each person's scalp was marked with a 3cm x 3cm area on both sides of the symmetrical region, with the left side being the sample area, and the right side being the control area. The sample area was applied with the composition of the present application (application example 1 and comparative application examples 1-15) to regulate the secretion of scalp oil, and the control area was applied with a general essence without the composition of the present application, each with 0.5ml. The skin oil tester Sebumeter SM815 was used to test and record the scalp oil content after 4, 6 and 8 hours of use, and 3 points were randomly selected from each area for testing, and the average value was taken, denoted as T4, T6 and T8 respectively. The oil control effect was evaluated by calculating the change rate of sebum secretion.

[0155] (5) Sebum secretion change rate (%) = [(T X样品 -T 0样品 )-(T X对照 -T 0对照 )] / (T X对照 -T 0对照 )*100%;

[0156] T0 is the initial scalp sebum content, T X is the scalp sebum content after X hours, and the test results are shown in Table 7:

[0157] Table 7 Test results of application example 1 for inhibiting the secretion of scalp oil

[0158]

[0159] Table 8 Inhibition of scalp essence of application example 1 and comparative application examples 1-15 on sebum secretion

[0160]

[0161] As can be seen from the test results of Table 8, compared with the control area smeared with the ordinary essence liquid not containing the composition of the present application, the sebum secretion of the scalp of the subjects was reduced by 36.23%, 28.93% and 28.04% respectively after 4h, 6h and 8h of using the scalp essence liquid of Application Example 1, indicating that the composition of Example 1 can significantly inhibit the sebum secretion of the scalp. Comparing Application Examples 1-15, due to the lack of one or two or three or four components of azelamide potassium, APN activity inhibitor, plankton extract and nicotinamide in the sebum secretion inhibitor, the decrease in the sebum secretion of the scalp after application is reduced to different degrees, indicating that azelamide potassium, APN activity inhibitor, plankton extract and nicotinamide in the composition have a synergistic effect, and the lack of one or two or three or four components will adversely affect the regulation of sebum secretion of the scalp. Therefore, the composition of the present application containing azelamide potassium, APN activity inhibitor, plankton extract and nicotinamide can significantly inhibit the sebum secretion of the scalp and reduce the oil content on the surface of the scalp.

[0162] The above detailed description of the present application has been made, but the present application is not limited to the above examples, and various changes can be made within the knowledge of those skilled in the art without departing from the spirit of the present application. Furthermore, the embodiments of the present application and the features in the embodiments can be combined with each other without conflict.

Claims

1. A composition for regulating scalp sebum secretion through multiple pathways, characterized in that, It contains the following ingredients by weight percentage: potassium azeloyl diglycinate 1-5%, APN activity inhibitor 1-5%, plankton extract 1-4%, niacinamide 1-2%, moisturizer 0.1-1.5%, and the balance being water; The APN activity inhibitor is one or both of the following: heather extract and black tea leaf extract.

2. The composition according to claim 1, characterized in that, The moisturizer includes 1, 3 At least one of propylene glycol, glycerol, butylene glycol, panthenol, sodium hyaluronate and its derivatives, trehalose, and xylitol glucoside.

3. A method for preparing the composition according to any one of claims 1 to 2, comprising the following steps: mixing the moisturizer, potassium azeloyl diglycinate, APN activity inhibitor, plankton extract, and nicotinamide evenly.

4. The use of the composition according to any one of claims 1 to 2 in the preparation of oil-controlling cosmetics.

5. The application according to claim 4, characterized in that, The oil-controlling cosmetics include skincare or cleansing products.

6. The application according to claim 5, characterized in that, The care products include one or more of the following: serum, conditioner, hair mask, and skin gel.

7. The application according to claim 5, characterized in that, The cleaning products include shampoo.

8. An oil-controlling cosmetic, wherein the oil-controlling cosmetic comprises the composition according to any one of claims 1 to 2.

9. The oil-controlling cosmetic according to claim 8, characterized in that, The oil-controlling cosmetic product comprises 5-15% by mass of the composition according to any one of claims 1-3.

10. The oil-controlling cosmetic according to claim 8, characterized in that, The oil-controlling cosmetics also contain excipients.

11. The oil-controlling cosmetic according to claim 10, characterized in that, The excipients include at least one of the following: thickener, pH adjuster, skin conditioner, penetration enhancer, and preservative.

12. The oil-controlling cosmetic according to claim 11, characterized in that, The thickener includes carbomer, acrylates / C10 At least one of the following: 30-alkyl alcohol acrylate crosspolymer, xanthan gum, and cellulose ether.

13. The oil-controlling cosmetic according to claim 11, characterized in that, The pH adjuster includes at least one of aminomethylpropanol, sodium hydroxide, potassium hydroxide, triethanolamine, arginine, citric acid, sodium citrate, lactic acid, and sodium lactate.

14. The oil-controlling cosmetic according to claim 11, characterized in that, The skin conditioning agent includes at least one of dipotassium glycyrrhizate, trehalose, and allantoin.

15. The oil-controlling cosmetic according to claim 11, characterized in that, The penetration enhancer includes at least one of ethanol, propylene glycol, and menthol.

16. The oil-controlling cosmetic according to claim 11, characterized in that, The preservatives include phenoxyethanol, ethylhexylglycerin, capryloyl hydroxamic acid, p-hydroxyacetophenone, caprylyl glycol, and 1,2-diethyltoluene. At least one of hexanediol.

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