Immunoassay device and method for automatic dilution of a sample

Abstract

The application relates to an immunological analysis device and a sample automatic dilution method. The immunological analysis device comprises a magnetic collection device, a gripper device and a sampling needle device. The magnetic collection device comprises a bearing part, and a sample adding position is arranged on the bearing part. The sample adding position is used for bearing a reaction cup. The gripper device is used for moving the reaction cup to the sample adding position. The sampling needle device can be close to or away from the magnetic collection device, and can add a preset reaction liquid into the reaction cup on the sample adding position. Since the immunological analysis device directly arranges the sample adding position on the bearing part to bear the reaction cup, uses the reaction cup at the sample adding position as a sample dilution container, and uses the gripper device to move the reaction cup, and uses the sampling needle device to add the sample and dilute, the immunological analysis device does not need to additionally design a special sample dilution device. The whole device has a simple structure, occupies a small space, has a low manufacturing cost, has a faster sample dilution speed, and has a higher sample test efficiency.

Description

Technical Field

[0001] This application relates to the field of medical testing equipment technology, and in particular to immunoassay devices and automatic sample dilution methods. Background Technology

[0002] Immunoassay technology has gained increasingly widespread application in recent years due to its advantages such as high sensitivity, good specificity, wide linear range, high throughput, strong platform scalability, and a rich menu of measurable items. During the detection process in immunoassay devices, sample dilution is required. However, most related technologies design a dedicated sample dilution device within the immunoassay apparatus, making the overall structure complex, space-consuming, and costly; alternatively, manual sample dilution is used, but this method reduces testing efficiency. Summary of the Invention

[0003] Therefore, it is necessary to provide an immunoassay device that addresses the problems of existing immunoassay devices being complex in structure, occupying a large space, having high manufacturing costs, and having low testing efficiency.

[0004] An immunoassay device comprising:

[0005] A magnetic collection device, comprising a support member having a sample loading position configured thereon for supporting a reaction vessel;

[0006] A gripper device, the gripper device being used to grip and move the reaction cup to the sample dispensing position;

[0007] The sampling needle device is capable of moving closer to or further away from the magnetic collection device and adding a preset reaction solution to the reaction cup at the sample application position.

[0008] In one embodiment, there are multiple sample application sites, and the multiple sample application sites are spaced apart.

[0009] In one embodiment, the immunoassay apparatus further includes a reagent sample loading device, which includes multiple reagent kits and multiple sample tubes;

[0010] Multiple of the aforementioned kits are used to carry different preset reagents, and multiple of the aforementioned sample tubes are used to carry different samples.

[0011] In one embodiment, the immunoassay apparatus further includes a mixing device;

[0012] The mixing device is configured with a mixing position for supporting the reaction cup; the mixing device is used to perform a mixing operation on the preset reaction liquid in the reaction cup.

[0013] The gripper device is also used to grip and move the reaction cup containing the preset reaction solution to the mixing position.

[0014] This application also provides an automatic sample dilution method based on the immunoassay apparatus described in any of the above embodiments, the automatic sample dilution method comprising:

[0015] The magnetic collection device is controlled to move along a first direction so that the first sample feeding position in the sample feeding position moves to a first preset position;

[0016] The first reaction cup is grasped and moved to the first sample dispensing position using the gripper device;

[0017] Control the movement of the sampling needle device and add a first preset reaction solution into the first reaction cup.

[0018] In one embodiment, the step of controlling the movement of the sampling needle device and adding a first preset reaction solution into the first reaction cup specifically includes:

[0019] Control the sampling needle device to move relative to the reagent sample loading device to a second preset position;

[0020] The sampling needle of the sampling needle device is controlled to draw in a first preset reaction solution at the reagent sample loading device;

[0021] Control the sampling needle device to move relative to the magnetic collection device to a third preset position;

[0022] The sampling needle is controlled to discharge the first preset reaction solution into the first reaction cup.

[0023] In one embodiment, after the step of controlling the sampling needle to discharge the aspirated first preset reaction solution into the first reaction cup, the method further includes:

[0024] The sampling needle device is moved to the cleaning pool and the sampling needle is cleaned.

[0025] In one embodiment, after the step of controlling the sampling needle to discharge the aspirated first preset reaction solution into the first reaction cup, the method further includes:

[0026] Control the magnetic collection device to move along the first direction to the first preset position.

[0027] In one embodiment, the step of controlling the movement of the sampling needle device and adding a first preset reaction solution to the first reaction cup further includes:

[0028] Control the magnetic collection device to move along the first direction to the fourth preset position.

[0029] In one embodiment, after the steps of controlling the movement of the sampling needle device and adding a first preset reaction solution into the first reaction cup, the method further includes:

[0030] The first reaction cup is grasped and moved to the mixing position of the mixing device using the gripper device;

[0031] The mixing device is used to mix the first preset reaction solution in the first reaction cup.

[0032] In one embodiment, the step of mixing the first preset reaction solution in the first reaction cup using the mixing device specifically includes:

[0033] The mixing device is controlled to oscillate eccentrically, causing the first reaction cup to shake.

[0034] In one embodiment, after the step of mixing the first preset reaction solution in the first reaction cup using the mixing device, the method further includes:

[0035] The first reaction cup is grasped and moved to the second sample dispensing position in the sample dispensing position by the gripper device;

[0036] The second reaction cup is grasped and moved to the first sample dispensing position using the gripper device;

[0037] Control the movement of the sampling needle device and add a second preset reaction solution into the second reaction cup.

[0038] In one embodiment, the step of controlling the movement of the sampling needle device and adding a second preset reaction solution into the second reaction cup specifically includes:

[0039] Control the sampling needle device to move relative to the reagent sample loading device to a second preset position;

[0040] The sampling needle of the sampling needle device is controlled to draw a second preset reagent into the reagent kit of the reagent sample loading device;

[0041] Control the sampling needle device to move relative to the magnetic collection device to a third preset position;

[0042] The sampling needle is controlled to draw in the first preset reaction solution from the first reaction cup;

[0043] The second preset reagent and the first preset reaction solution drawn in by the sampling needle are discharged into the second reaction cup.

[0044] In one embodiment, after the step of draining the second preset reagent and the first preset reaction solution drawn in by the sampling needle into the second reaction cup, the method further includes:

[0045] The sampling needle device is moved to the cleaning pool and the sampling needle is cleaned.

[0046] In one embodiment, after the step of controlling the sampling needle to draw in the first preset reaction liquid from the first reaction cup, the method further includes:

[0047] The magnetic collection device is controlled to move along the first direction so that the second sample application position moves to the first preset position;

[0048] The first reaction cup is grasped and moved to the waste cup channel by the gripper device.

[0049] In one embodiment, after the steps of controlling the movement of the sampling needle device and adding the second preset reaction solution into the second reaction cup, the method further includes:

[0050] The second reaction cup is grasped and moved to the mixing position of the mixing device using the gripper device;

[0051] The mixing device is used to mix the second preset reaction solution in the second reaction cup.

[0052] In one embodiment, prior to the step of grasping and moving the second reaction cup to the mixing position of the mixing device using the gripper device, the method further includes:

[0053] The magnetic collection device is controlled to move along the first direction so that the first sample application position moves to the first preset position.

[0054] In one embodiment, the step of mixing the second preset reaction solution in the second reaction cup using the mixing device specifically includes:

[0055] The mixing device is controlled to oscillate eccentrically to shake the second reaction cup.

[0056] When performing sample dilution using the aforementioned immunoassay analyzer, the reaction cup is first moved to the sample dispensing position on the carrier of the magnetic collection device using a gripper. Then, a pre-set reaction solution is added to the reaction cup at the dispensing position using a sampling needle, thus completing the sample dilution within the reaction cup. Because this immunoassay analyzer directly constructs a sample dispensing position on the carrier of the magnetic collection device to hold the reaction cup, and uses the reaction cup at the dispensing position as the sample dilution container, while simultaneously utilizing the gripper to move the reaction cup and the sampling needle to add and dilute the sample, the entire immunoassay analyzer does not require a separate dedicated sample dilution device. Therefore, the overall structure of the immunoassay analyzer is relatively simple, occupies less space, and has a lower manufacturing cost. Furthermore, compared to manual dilution methods, sample dilution is faster, and sample testing efficiency is higher. Attached Figure Description

[0057] Figure 1 This is a first schematic diagram of an immunoassay apparatus provided in some embodiments of this application.

[0058] Figure 2 for Figure 1 The second schematic diagram of the immunoassay device is shown.

[0059] Figure 3 for Figure 1 A top view of the immunoassay apparatus shown.

[0060] Figure 4 for Figure 1 A schematic diagram of the magnetic collection device in the shown immunoassay apparatus.

[0061] Figure 5 for Figure 1 A schematic diagram of the sampling needle device in the shown immunoassay apparatus.

[0062] Figure 6 A flowchart of an automatic sample dilution method provided for some embodiments of this application.

[0063] Reference numerals: 100-Magnetic collection device; 110-Carrier; 111-Sampling position; 1111-First sampling position; 1112-Second sampling position; 112-Magnetic collection position; 120-Magnet; 200-Sampling needle device; 210-Sampling needle; 300-Mixing device; 310-Mixing position; 400-Holder device; 500-Reagent sample loading device; 510-Reagent kit; 520-Sample tube; 600-Reaction cup loading device; 700-Waste cup channel; 800-Reaction cup; Detailed Implementation

[0064] To make the above-mentioned objectives, features, and advantages of this application more apparent and understandable, the specific embodiments of this application are described in detail below with reference to the accompanying drawings. Many specific details are set forth in the following description to provide a thorough understanding of this application. However, this application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar modifications without departing from the spirit of this application. Therefore, this application is not limited to the specific embodiments disclosed below.

[0065] In the description of this application, it should be understood that if terms such as "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential" appear, these terms indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application.

[0066] Furthermore, where the terms "first" and "second" appear, these terms are for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined with "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this application, where the term "multiple" appears, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0067] In this application, unless otherwise expressly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise expressly limited. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0068] In this application, unless otherwise expressly specified and limited, the use of descriptions such as "above" or "below" the second feature indicates that the first and second features are in direct contact or indirect contact via an intermediate medium. Furthermore, "above," "on top of," and "over" the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. Similarly, "below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.

[0069] It should be noted that if an element is referred to as being "fixed to" or "set on" another element, it can be directly on the other element or there may be an intervening element. If an element is considered to be "connected to" another element, it can be directly connected to the other element or there may be an intervening element. If so, the terms "vertical," "horizontal," "upper," "lower," "left," "right," and similar expressions used in this application are for illustrative purposes only and do not represent the only possible implementation.

[0070] Immunoassay technology has gained increasingly widespread application in recent years due to its advantages such as high sensitivity, good specificity, wide linear range, high throughput, strong platform scalability, and rich menu of measurable items. During the detection process in immunoassay devices, sample dilution is required. However, most related technologies design a dedicated sample dilution device within the immunoassay device, making the overall structure complex, space-consuming, and costly; alternatively, manual sample dilution is used, but this method reduces testing efficiency. To address these issues, this application provides an immunoassay device.

[0071] See Figures 1-5 , Figure 1 A first schematic diagram of an immunoassay apparatus provided in some embodiments of this application is shown. Figure 2 It shows Figure 1 The second schematic diagram of the immunoassay device is shown. Figure 3 It shows Figure 1 A top view of the immunoassay apparatus shown. Figure 4 It shows Figure 1 A schematic diagram of the magnetic collection device 100 in the shown immunoassay apparatus. Figure 5 It shows Figure 1The diagram shows a sampling needle device 200 in an immunoassay apparatus. An embodiment of this application provides an immunoassay apparatus including a magnetic collection device 100, a gripper device 400, and a sampling needle device 200. The magnetic collection device 100 includes a support member 110 with a sample application position 111 for supporting a reaction cup 800. The gripper device 400 is used to grasp and move the reaction cup 800 to the sample application position 111. The sampling needle device 200 can move closer to or further away from the magnetic collection device 100 and add a preset reaction solution to the reaction cup 800 at the sample application position 111.

[0072] When performing sample dilution using the aforementioned immunoassay apparatus, the reaction cup 800 is first moved to the sample dispensing position 111 on the carrier 110 of the magnetic collection device 100 using the gripper device 400. Then, the sampling needle device 200 adds a pre-set reaction solution to the reaction cup 800 at the sample dispensing position 111, thus completing the sample dilution within the reaction cup 800. Because this immunoassay apparatus directly constructs the sample dispensing position 111 on the carrier 110 of the magnetic collection device 100 to support the reaction cup 800, and uses the reaction cup 800 at the sample dispensing position 111 as the sample dilution container, while the gripper device 400 moves the reaction cup 800 and the sampling needle device 200 adds and dilutes the sample, the entire immunoassay apparatus does not require a separate dedicated sample dilution device. Therefore, the structure of the entire immunoassay apparatus is relatively simple, occupies less space, and has a lower cost. Furthermore, compared to manual dilution methods, sample dilution is faster and sample testing efficiency is higher.

[0073] It should be noted that there are no restrictions on the type of preset reaction solution added to the reaction cup 800 at the sample dispensing position 111. It can be a sample and / or a preset reagent, or it can be a diluent. Different preset reaction solutions are added to the reaction cup 800 located in the sample dispensing position 111 more than 200 times using the sampling needle device, thereby realizing the sample dispensing and dilution operations within the reaction cup 800. There are no special limitations on this.

[0074] Please see Figure 4In some embodiments, the carrier 110 of the magnetic collection device 100 is further provided with a plurality of magnetic collection positions 112, and magnets 120 are disposed on the outer periphery of the plurality of magnetic collection positions 112. The magnetic collection positions 112 are used to support the reaction cup 800 containing magnetic beads and magnetic bead complex. After the reagent in the reaction cup 800 containing magnetic beads and magnetic bead complex has completed the incubation reaction, the reaction cup 800 is grasped and moved to the magnetic collection position 112, and under the action of the magnetic field of the magnet 120, the magnetic beads and magnetic bead complex are collected on the cup wall of the reaction cup 800, and the part of the reaction material that is not bound to the magnetic beads is removed. The above describes the conventional structure and function of the magnetic collection device 100. In this application, the conventional structure of the magnetic collection device 100 is improved by adding a sample loading position 111 to the carrier 110 of the magnetic collection device 100, thereby completing the sample dilution and loading operation at the magnetic collection device 100, making the overall structure of this immunoassay device more compact, smaller in size, and lower in cost.

[0075] Please see Figure 4 In some embodiments, there are multiple sample dispensing positions 111, which are spaced apart. By providing multiple sample dispensing positions 111, different reaction cups 800 can be placed on each of the multiple dispensing positions 111, thereby enabling multiple sample dilution thread operations to be performed simultaneously during the sample dilution process, further improving the efficiency of sample dilution. Please refer to [link to relevant documentation]. Figure 4 In one specific embodiment, there are two sample application positions 111. The two sample application positions 111 are spaced apart along a first direction.

[0076] Please see Figures 1-3 In some embodiments, the immunoassay apparatus further includes a reagent sample loading device 500, which includes a plurality of reagent kits 510 and sample tubes 520; the plurality of reagent kits 510 are used to carry different preset reagents. The plurality of sample tubes 520 are used to carry different samples. By carrying different preset reagents in the plurality of reagent kits 510 and carrying different test samples in the plurality of sample tubes 520, the immunoassay apparatus can adapt to different testing needs.

[0077] Please see Figure 3 In some embodiments, the immunoassay apparatus further includes a mixing device 300, which is configured with a mixing position 310 for holding a reaction cup 800; the mixing device 300 is used to mix a preset reaction solution in the reaction cup 800; and the gripper device 400 is also used to grip and move the reaction cup 800 containing the preset reaction solution into the mixing position 310.

[0078] Please see Figure 3In some embodiments, the immunoassay apparatus further includes a reaction cup loading device 600 for loading and buffering new reaction cups 800.

[0079] Please see Figure 3 In some embodiments, the immunoassay apparatus further includes a waste cup channel 700 for buffering used waste reaction cups 800, thereby facilitating subsequent collection and processing of the waste reaction cups 800.

[0080] This application also provides an automatic sample dilution method; please refer to [link / reference]. Figure 6 , Figure 6 This is a flowchart of an auto-dilution method for samples provided in some embodiments of this application. Based on the immunoassay apparatus described in any of the above embodiments, the auto-dilution method for samples includes:

[0081] S10: Control the magnetic collection device 100 to move along a first direction, specifically, the first direction is... Figure 2 and Figure 3 The direction of xx' in the sample application position 1111 is used to move the first sample application position 1111 in the sample application position 111 to the first preset position;

[0082] S20: The first reaction cup is grasped and moved to the first sample loading position 1111 by the gripper device 400;

[0083] S30: Control the movement of the sampling needle device 200 and add the first preset reaction solution into the first reaction cup.

[0084] When sample dilution is required, the magnetic collection device 100 is first moved along a first direction, so that the first sample loading position 1111 moves to a first preset position, which is the preset position for the reaction cup 800. Then, the gripper device 400 grabs the first reaction cup from the reaction cup loading device 600 of the immunoassay apparatus and moves it to the first sample loading position 1111. The sampling needle device 200 then adds the first preset reaction solution to the first reaction cup at the first sample loading position 1111, thereby completing the sample dilution within the reaction cup 800. The automatic sample dilution method provided in this application uses the first sample loading position 1111 constructed on the carrier 110 of the magnetic collection device 100 to support the first reaction cup, and uses the first reaction cup as a container for sample dilution. Simultaneously, the gripper device 400 moves the first reaction cup, and the sampling needle device 200 adds and dilutes the sample within the first reaction cup. Therefore, the entire sample dilution process does not require the design of a separate sample dilution device, making it simpler and more convenient. At the same time, compared with the manual dilution method, the sample dilution speed is faster and the sample testing efficiency is higher.

[0085] It should be noted that there are no restrictions on the type of the first preset reaction solution added to the first reaction cup; it can be a sample and / or a preset reagent, or it can be a diluent. Different first preset reaction solutions can be added to the first reaction cup more than 200 times using the sampling needle device, thereby enabling sample addition and dilution operations within the first reaction cup; no special limitations are imposed on this.

[0086] In some embodiments, step S30 of the automatic sample dilution method, which involves controlling the movement of the sampling needle device 200 and adding a first preset reaction solution to the first reaction cup, specifically includes:

[0087] The sampling needle device 200 is controlled to move relative to the reagent sample loading device 500 to a second preset position. The sampling needle device 200 is controlled to move along... Figure 2 The sampling needle device 200 moves relative to the reagent sample loading device 500 in the directions of xx', yy' and zz', thereby moving to the second preset position, which is above the reagent kit 510 or sample tube.

[0088] The sampling needle 210 of the sampling needle device 200 is controlled to draw in a first preset reaction solution at the reagent sample loading device 500. It should be noted that the first preset reaction solution can be a first preset reagent drawn in from the reagent kit 510 of the reagent sample loading device 500, or a sample drawn in from the sample tube 520 of the reagent sample loading device 500; it can also be a mixture of the first preset reagent and the sample. When the sampling needle device 200 moves to the second preset position, the sampling needle 210 is controlled to move up and down along the z' direction, thereby allowing the sampling needle 210 to extend into the reagent kit 510 and draw in the first preset reagent within the reagent kit 510, and / or extend into the sample tube 520 and draw in the sample within the sample tube 520.

[0089] The sampling needle device 200 is controlled to move relative to the magnetic collection device 100 to a third preset position. After the sampling needle 210 draws in the first preset reaction liquid, the sampling needle device 200 is controlled to move along... Figure 2 The sampling needle device 200 moves relative to the magnetic collection device 100 in the directions of xx', yy' and zz', thereby moving to the third preset position so that the sampling needle 210 can discharge the first preset reaction liquid into the first reaction cup.

[0090] The sampling needle 210 is controlled to discharge the first preset reaction solution into the first reaction cup. When the sampling needle device 200 moves relative to the magnetic collection device 100 to the third preset position, the sampling needle 210 is controlled to move along... Figure 2 The zz' direction moves up and down, and controls the air pressure inside the sampling needle 210, so that the sampling needle 210 can discharge the first preset reaction liquid into the first reaction cup.

[0091] In some embodiments, after controlling the sampling needle 210 to discharge the first preset reaction solution into the first reaction cup, the method further includes: controlling the sampling needle device 200 to move to a cleaning tank (not shown) and cleaning the sampling needle 210. After the first preset reaction solution is discharged into the first reaction cup, the sampling needle device 200 is controlled to move along... Figure 2 and Figure 3 The sampling needle device 200 moves in the xx' and yy' directions, thereby moving to the cleaning pool, and the sampling needle 210 is controlled to move along... Figure 2 The sampling needle 210 moves in the zz' direction, causing it to move into the cleaning pool. The cleaning pump, inner wall cleaning valve, vacuum pump and other fluid components in the cleaning pool clean the inner and outer walls of the sampling needle 210, avoiding reagent or sample residue in the sampling needle 210 and facilitating the next liquid aspiration and drainage operation.

[0092] In some embodiments, after controlling the sampling needle 210 to discharge the first preset reaction liquid into the first reaction cup, the method further includes: controlling the magnetic collection device 100 to move along a first direction to a first preset position. After the sampling needle 210 discharges the first preset reaction liquid into the first reaction cup, the magnetic collection device 100 is then controlled to move along the first direction to the first preset position, which is the transfer position of the reaction cup 800. This facilitates easier handling and movement during subsequent mixing of the first preset reaction liquid in the first reaction cup.

[0093] In some embodiments, before step S30: controlling the sampling needle device 200 to move and adding the first preset reaction liquid to the first reaction cup, the method further includes: controlling the magnetic collection device 100 to move along the first direction to a fourth preset position. When it is necessary to add the first preset reaction liquid to the first reaction cup, the magnetic collection device 100 is first moved along the first direction to the fourth preset position, which is the liquid transfer position. At this position, it is convenient for the sampling needle 210 to move along the first direction. Figure 2 The zz' direction in the middle moves up and down, which facilitates the addition and dilution of the first reaction vessel.

[0094] In some embodiments, after step S30: controlling the movement of the sampling needle device 200 and adding the first preset reaction solution into the first reaction cup, the method further includes:

[0095] S40: The gripper device 400 grasps and moves the first reaction cup to the mixing position 310 of the mixing device 300; S50: The mixing device 300 mixes the first preset reaction solution in the first reaction cup. After the first preset reaction solution is added to the first reaction cup, the gripper device 400 grasps the first reaction cup from the first sample addition position 1111 and moves it to the mixing position 310 of the mixing device. Then, the mixing device 300 mixes the first preset reaction solution in the first reaction cup, thereby completing the sample addition, dilution, and mixing operation in the first reaction cup. This makes the first preset reaction solution in the first reaction cup more uniform, and the final analysis and detection results are more accurate.

[0096] In some embodiments, step S50, mixing the first preset reaction liquid in the first reaction cup using the mixing device 300, specifically includes: controlling the mixing device 300 to eccentrically oscillate, causing the first reaction cup to shake. The eccentric oscillation of the mixing device 300 causes the first reaction cup to shake, thereby mixing the first preset reaction liquid within the first reaction cup during the shaking process.

[0097] In some embodiments, after step S50: mixing the first preset reaction liquid in the first reaction cup by the mixing device 300, the method further includes: step S60: using the gripper device 400 to grab and move the first reaction cup to the second sample addition position 1112 in the sample addition position 111; step S70: using the gripper device 400 to grab and move the second reaction cup to the first sample addition position 1111; step S80: controlling the sampling needle device 200 to move and adding the second preset reaction liquid into the second reaction cup.

[0098] After the first preset reaction solution is mixed, the gripper device 400 moves the first reaction cup to the second sample addition position 1112 to make room in the first sample addition position 1111. Then, the gripper device 400 moves a new reaction cup 800, which is the second reaction cup, to the first sample addition position 1111. The sampling needle device 200 is then moved to add the second preset reaction solution to the second reaction cup, thereby realizing the sample addition or dilution operation in the second reaction cup.

[0099] In some embodiments, step S80: controlling the movement of the sampling needle device 200 and adding a second preset reaction solution to the second reaction cup specifically includes: controlling the sampling needle device 200 to move relative to the reagent sample loading device 500 to a second preset position; controlling the sampling needle 210 of the sampling needle device 200 to draw in a second preset reagent from the reagent kit 510 of the reagent sample loading device 500; controlling the sampling needle device 200 to move relative to the magnetic collection device 100 to a third preset position; controlling the sampling needle 210 to draw in a first preset reaction solution from the first reaction cup; and discharging the second preset reagent and the first preset reaction solution drawn in by the sampling needle 210 into the second reaction cup.

[0100] When it is necessary to add the second preset reaction solution to the second reaction cup, the sampling needle device 200 is controlled to move along... Figure 2 and Figure 3 The sampling needle device 200 moves relative to the reagent sample loading device 500 in the xx' and yy' directions, thereby moving to the second preset position. Then, the sampling needle 210 is controlled to move along... Figure 2 The sampling needle 210 moves up and down in the zz' direction to draw in the second preset reagent into the reagent kit 510; then the sampling needle device 200 is controlled to move along... Figure 2 and Figure 3 The sampling needle 210 moves along the xx' and yy' directions to move relative to the magnetic collection device 100 to the third preset position, and then controls the sampling needle 210 to move along... Figure 2 The sampling needle 210 moves up and down in the zz' direction to draw in the first preset reaction solution from the first reaction cup; and finally, it discharges all the second preset reagent and the first preset reaction solution drawn in by the sampling needle 210 into the second reaction cup, at which point the second reaction cup contains a mixture of the second preset reagent and the first preset reaction solution. It should be noted that the second preset reaction solution is the mixture of the second preset reagent and the first preset reaction solution.

[0101] In some embodiments, after the step of adding the second preset reagent and the first preset reaction solution drawn in by the sampling needle 210 into the second reaction cup, the method further includes: controlling the sampling needle device 200 to move to the cleaning pool and performing a cleaning operation on the sampling needle 210. After the sampling needle 210 adds the second preset reagent and the first preset reaction solution drawn in to the second reaction cup, the sampling needle device 200 is controlled to move along... Figure 2 and Figure 3 The sampling needle device 200 moves in the xx' and yy' directions, thereby moving to the cleaning pool, and the sampling needle 210 is controlled to move along... Figure 2The sampling needle 210 moves up and down in the zz' direction, causing it to move into the cleaning pool. The cleaning pump, inner wall cleaning valve, vacuum pump and other fluid components in the cleaning pool clean the inner and outer walls of the sampling needle 210, avoiding reagent or sample residue in the sampling needle 210 and facilitating the next liquid aspiration and drainage operation.

[0102] In some embodiments, after the step of controlling the sampling needle 210 to draw in the first preset reaction liquid in the first reaction cup, the method further includes: controlling the magnetic collection device 100 to move along a first direction so that the second sample application position 1112 moves to a first preset position; and using the gripper device 400 to grasp and move the first reaction cup to the waste cup channel 700. When the sampling needle 210 draws in the first preset reaction liquid from the first reaction cup, the magnetic collection device 100 is then controlled to move along the first direction, that is... Figure 2 and Figure 3 The second sample loading position 1112, which carries the first reaction cup, moves to the first preset position, which is the reaction cup 800 transfer position. This makes it easier for the gripper device 400 to grab and move the first reaction cup to the waste cup channel 700, and it is less likely to cause motion interference.

[0103] In some embodiments, after step S80: controlling the movement of the sampling needle device 200 and adding the second preset reaction solution to the second reaction cup, the method further includes: S90: using the gripper device 400 to grasp and move the second reaction cup to the mixing position 310 of the mixing device 300; S100: using the mixing device 300 to mix the second preset reaction solution in the second reaction cup. After the second preset reaction solution is added to the second reaction cup, the gripper device 400 grasps and moves the second reaction cup to the mixing position 310 of the mixing device 300, thereby using the mixing device 300 to mix the second preset reaction solution. This completes the sample dilution and mixing operation in the second reaction cup, making the second preset reaction solution in the second reaction cup more uniform, and ultimately making the analysis and detection results more accurate. It should be noted that since the second preset reaction solution is a mixture of the first preset reaction solution and the third preset reaction solution, mixing by the mixing device 300 can make the mixture of the first preset reaction solution and the third mixed reagent more uniform.

[0104] In some embodiments, before step S90: moving the second reaction cup to the mixing position 310 of the mixing device 300 using the gripper device 400, the method further includes: controlling the magnetic collection device 100 to move along a first direction so that the first sample loading position 1111 moves to a first preset position. Before moving the second reaction cup to the mixing position 310 of the mixing device 300, the magnetic collection device 100 is first controlled to move along the first direction so that the first sample loading position 1111 carrying the second reaction cup is at the first preset position, which is the displacement position of the reaction cup 800, and then the gripper device 400 is used to move it. This makes the gripping and moving process of the gripper device 400 more convenient and less prone to motion interference.

[0105] In some embodiments, step S100, which involves mixing the second preset reaction liquid in the second reaction cup using the mixing device 300, specifically includes controlling the mixing device 300 to eccentrically oscillate, thereby shaking the second reaction cup. The eccentric oscillation of the mixing device 300 causes the second reaction cup to shake, thus mixing the second preset reaction liquid within the second reaction cup during the shaking process.

[0106] It should be understood that, in the embodiments of this application, at least some steps in the preparation method may include multiple steps or multiple stages. These steps or stages are not necessarily completed at the same time, but may be executed at different times. The execution order of these steps or stages is not necessarily sequential, but may be executed in turn or alternately with other steps or at least some of the steps or stages in other steps.

[0107] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0108] The embodiments described above are merely illustrative of several implementation methods of this application, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent application. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this application, and these all fall within the protection scope of this application. Therefore, the protection scope of this patent application should be determined by the appended claims.

Claims

1. An immunoassay device, characterized in that, The immunoassay device includes: A magnetic collection device is movable along a first direction; the magnetic collection device includes a carrier member, on which a plurality of sample loading positions and a plurality of magnetic collection positions are constructed, the sample loading positions being used to support a reaction vessel; the sample loading positions include a first sample loading position and a second sample loading position spaced apart along the first direction. A gripper device, the gripper device being used to grip and move the reaction cup to the sample dispensing position; A sampling needle device, which can move closer to or further away from the magnetic collection device and add a preset reaction solution to the reaction cup at the sample application position; Reaction cup apparatus, used for assembling and buffering new reaction cups; When diluting the sample, the magnetic collection device moves along the first direction so that the first sample loading position moves to the first preset position; the gripper device grabs the first reaction cup from the reaction cup loading device and moves it to the first sample loading position; the sampling needle device adds the first preset reaction solution to the first reaction cup at the first sample loading position and completes the sample dilution in the reaction cup; After the first preset reaction solution is mixed, the gripper device moves the first reaction cup to the second sample dispensing position, so that the gripper device can move a new reaction cup to the first sample dispensing position.

2. The immunoassay device according to claim 1, characterized in that, The immunoassay device also includes a reagent sample loading device, which includes multiple reagent kits and multiple sample tubes; Multiple of the aforementioned reagent kits are used to carry different pre-set reagents; multiple of the aforementioned sample tubes are used to carry different samples.

3. The immunoassay device according to any one of claims 1-2, characterized in that, The immunoassay device also includes a mixing device; The mixing device is configured with a mixing position for supporting the reaction cup; the mixing device is used to perform a mixing operation on the preset reaction liquid in the reaction cup. The gripper device is also used to grip and move the reaction cup containing the preset reaction solution to the mixing position.

4. An automatic sample dilution method, characterized in that, Based on the immunoassay apparatus according to any one of claims 1-3, the automatic sample dilution method comprises: The magnetic collection device is controlled to move along a first direction so that the first sample feeding position in the sample feeding position moves to a first preset position; The first reaction cup is grasped and moved to the first sample dispensing position using the gripper device; Control the movement of the sampling needle device and add a first preset reaction solution into the first reaction cup.

5. The automatic sample dilution method according to claim 4, characterized in that, The steps of controlling the movement of the sampling needle device and adding the first preset reaction solution into the first reaction cup specifically include: Control the sampling needle device to move relative to the reagent sample loading device to a second preset position; The sampling needle of the sampling needle device is controlled to draw in a first preset reaction solution at the reagent sample loading device; Control the sampling needle device to move relative to the magnetic collection device to a third preset position; The sampling needle is controlled to discharge the first preset reaction solution into the first reaction cup.

6. The automatic sample dilution method according to claim 5, characterized in that, After the step of controlling the sampling needle to discharge the first preset reaction solution into the first reaction cup, the method further includes: The sampling needle device is moved to the cleaning pool and the sampling needle is cleaned.

7. The automatic sample dilution method according to claim 5, characterized in that, After the step of controlling the sampling needle to discharge the first preset reaction solution into the first reaction cup, the method further includes: Control the magnetic collection device to move along the first direction to the first preset position.

8. The automatic sample dilution method according to claim 4, characterized in that, Before the step of controlling the movement of the sampling needle device and adding the first preset reaction solution into the first reaction cup, the method further includes: Control the magnetic collection device to move along the first direction to the fourth preset position.

9. The automatic sample dilution method according to any one of claims 4-8, characterized in that, After the steps of controlling the movement of the sampling needle device and adding the first preset reaction solution into the first reaction cup, the method further includes: The first reaction cup is grasped and moved to the mixing position of the mixing device using the gripper device; The mixing device is used to mix the first preset reaction solution in the first reaction cup.

10. The automatic sample dilution method according to claim 9, characterized in that, The step of mixing the first preset reaction solution in the first reaction cup using the mixing device specifically includes: The mixing device is controlled to oscillate eccentrically, causing the first reaction cup to shake.

11. The automatic sample dilution method according to claim 9, characterized in that, After the step of mixing the first preset reaction solution in the first reaction cup using the mixing device, the method further includes: The first reaction cup is grasped and moved to the second sample dispensing position in the sample dispensing position using the gripper device; The second reaction cup is grasped and moved to the first sample dispensing position using the gripper device; Control the movement of the sampling needle device and add a second preset reaction solution into the second reaction cup.

12. The automatic sample dilution method according to claim 11, characterized in that, The steps of controlling the movement of the sampling needle device and adding the second preset reaction solution into the second reaction cup specifically include: Control the sampling needle device to move relative to the reagent sample loading device to a second preset position; The sampling needle of the sampling needle device is controlled to draw a second preset reagent into the reagent kit of the reagent sample loading device; Control the sampling needle device to move relative to the magnetic collection device to a third preset position; The sampling needle is controlled to draw in the first preset reaction solution from the first reaction cup; The second preset reagent and the first preset reaction solution drawn in by the sampling needle are discharged into the second reaction cup.

13. The automatic sample dilution method according to claim 12, characterized in that, After the step of draining the second preset reagent and the first preset reaction solution drawn in by the sampling needle into the second reaction cup, the method further includes: The sampling needle device is moved to the cleaning pool and the sampling needle is cleaned.

14. The automatic sample dilution method according to claim 12, characterized in that, After the step of controlling the sampling needle to draw in the first preset reaction liquid from the first reaction cup, the method further includes: The magnetic collection device is controlled to move along the first direction so that the second sample application position moves to the first preset position; The first reaction cup is grasped and moved to the waste cup channel by the gripper device.

15. The automatic sample dilution method according to claim 11, characterized in that, After the steps of controlling the movement of the sampling needle device and adding the second preset reaction solution into the second reaction cup, the method further includes: The second reaction cup is grasped and moved to the mixing position of the mixing device using the gripper device; The mixing device is used to mix the second preset reaction solution in the second reaction cup.

16. The automatic sample dilution method according to claim 15, characterized in that, Before the step of grasping and moving the second reaction cup to the mixing position of the mixing device using the gripper device, the method further includes: The magnetic collection device is controlled to move along the first direction so that the first sample application position moves to the first preset position.

17. The automatic sample dilution method according to claim 15, characterized in that, The step of mixing the second preset reaction solution in the second reaction cup using the mixing device specifically includes: The mixing device is controlled to oscillate eccentrically to shake the second reaction cup.

Images