A hydroponic nutrient solution for cultivating forage germplasm resources and its preparation method and use

Abstract

The present invention relates to the technical field of germplasm resource cultivation, and specifically discloses a hydroponic nutrient solution for forage germplasm resource cultivation, and a preparation method and use thereof. The hydroponic nutrient solution is made of the following components: calcium nitrate tetrahydrate, potassium nitrate, ammonium dihydrogen phosphate, magnesium sulfate heptahydrate, disodium ferric ethylenediaminetetraacetate, boric acid, manganese sulfate, copper sulfate, zinc sulfate, sodium molybdate, and composite fermentation liquid; the composite fermentation liquid is prepared according to the following steps: mixing rice washing water and aloe juice, adding a composite bacterial agent for fermentation, the composite bacterial agent is composed of plant lactobacillus, candid mold and bacillus thuringiensis; adding chitosan to the obtained fermentation liquid, standing, and collecting the supernatant, that is, the composite fermentation liquid. The hydroponic nutrient solution provided by the present invention is simple to prepare, low in cost, not easy to breed bacteria, not easy to produce precipitation, can promote the growth of plants to be lush and strong, meet the nutritional needs of a variety of forage growth, and can effectively prevent the occurrence of root rot in hydroponic plants.

Description

Technical Field

[0001] The invention relates to the technical field of germplasm resource cultivation, and specifically discloses a hydroponic nutrient solution for cultivating forage germplasm resources, a preparation method thereof, and uses thereof. Background Art

[0002] Germplasm resources play a very important role in plant genetics. For forage grasses, their germplasm includes wild types, closely related species and artificially cultivated varieties. Storage research on these varieties is an important measure for biological protection and utilization. Laboratory cultivation is an important process in the research of forage grass germplasm resources. General cultivation experiments refer to the method of cultivating plants in a specific environment under artificially controlled conditions by placing the growth medium in a container and placing it in a greenhouse, artificial climate chamber or climate chamber. This method can strictly control environmental conditions such as water, nutrients, temperature, and light. It is a commonly used experimental method in the evaluation and identification of traits, and the study and exploration of physiological and biochemical mechanisms of simulated crop cultivation.

[0003] Cultivation tests can be divided into soil culture, gravel culture and solution culture according to the different growth media filled. Solution culture, also known as hydroponics, is a method of cultivating plants in artificially prepared solutions containing all or part of the nutrient elements. It has the following characteristics: ① fast plant growth; ② medium flow, uniform nutrient distribution, and the phenotype of plants is more likely to remain consistent, which is conducive to reducing the background difference in resource evaluation; ③ saving space, increasing the throughput of evaluation and identification, and improving scientific research efficiency; ④ clean and odorless, not easy to produce soil-borne diseases and pests; ⑤ easy to carry out various experimental treatments, etc., and is becoming more and more popular among scientific researchers.

[0004] Although hydroponic plants are convenient for various experimental treatments during germplasm cultivation or evaluation and identification, the nutrients in the water cannot meet the growth needs of the plants after a long period of cultivation, so it is necessary to supplement the plants with nutrients. General fertilizers directly put into water will not only bring a pungent smell, but also burn the plants due to excessive fertilizer. Moreover, chemical fertilizers discharged into the environment will threaten the soil and water environment, livestock and even human health through enrichment.

[0005] The existing hydroponic nutrient solution has complex ingredients, and consumers need to purchase a variety of different chemical reagents to meet the formulation requirements. In addition, the existing nutrient solution cannot combat the problem of pathogenic microorganisms infecting crop roots. Therefore, it is of great significance to develop a hydroponic nutrient solution with easy-to-obtain raw materials, comprehensive nutrient supply and demand, green and environmentally friendly, and able to combat plant root rot. Summary of the invention

[0006] In view of the deficiencies in the prior art, the present invention provides a hydroponic nutrient solution for cultivating forage germplasm resources, which has simple and readily available raw materials, a simple preparation process, and can effectively combat pathogenic microorganisms that infect plant roots.

[0007] The hydroponic nutrient solution for a forage germplasm resource cultivation experiment provided by the present invention is prepared from the following components: 372-417 mg / L of calcium nitrate tetrahydrate, 314-349 mg / L of potassium nitrate, 73-102 mg / L of ammonium dihydrogen phosphate, 262-283 mg / L of magnesium sulfate heptahydrate, 45.8-62.1 mg / L of disodium ferric ethylenediaminetetraacetate, 2.55-2.71 mg / L of boric acid, 2.06-2.14 mg / L of manganese sulfate, 0.09-0.13 mg / L of copper sulfate, 0.32-0.44 mg / L of zinc sulfate, 0.02-0.03 mg / L of sodium molybdate, and 305-450 mL / L of composite fermentation liquid;

[0008] The composite fermentation broth is prepared according to the following steps:

[0009] 10 to 20 parts of rice washing water and 8 to 12 parts of aloe vera juice are mixed, and a composite bacterial agent is added at an inoculum amount of 0.8 to 2 wt % for fermentation, wherein the composite bacterial agent is composed of Lactobacillus plantarum, Geotrichum candidum and Bacillus thuringiensis;

[0010] Chitosan is added to the obtained fermentation liquid, the mixture is allowed to stand, and the supernatant is collected to obtain the composite fermentation liquid.

[0011] Preferably, the hydroponic nutrient solution is made of the following components: 384 mg / L calcium nitrate tetrahydrate, 335 mg / L potassium nitrate, 87 mg / L ammonium dihydrogen phosphate, 274 mg / L magnesium sulfate heptahydrate, 51.8 mg / L disodium ferric ethylenediaminetetraacetate, 2.63 mg / L boric acid, 2.06 mg / L manganese sulfate, 0.11 mg / L copper sulfate, 0.38 mg / L zinc sulfate, 0.02 mg / L sodium molybdate, and 420 mL / L composite fermentation liquid;

[0012] The composite fermentation liquid is prepared by mixing 18 parts of rice washing water and 10 parts of aloe juice, and adding a composite bacterial agent at an inoculation amount of 1.5 wt % for fermentation.

[0013] Preferably, the amount of chitosan added is 1.0-2.5% based on the total amount of the fermentation broth.

[0014] Preferably, the composite bacterial agent is composed of Lactobacillus plantarum, Geotrichum candidum and Bacillus thuringiensis in equal proportions, and the number of effective live bacteria in the composite bacterial agent is 5 to 8×10 9 cfu / mL.

[0015] Preferably, the fermentation is carried out at 32-40° C. for 28-36 hours.

[0016] Preferably, the standing is at 10-25° C. for 6-10 hours.

[0017] Preferably, the rice washing water is the filtrate obtained by adding water equivalent to 8 to 10 times the total mass of rice or millet or a mixture of the two and soaking for 0.5 to 1 hour.

[0018] The present invention also provides a method for preparing a hydroponic nutrient solution for cultivating forage germplasm resources, which is specifically carried out according to the following steps:

[0019] Weigh calcium nitrate tetrahydrate, potassium nitrate, ammonium dihydrogen phosphate, magnesium sulfate heptahydrate, disodium ferric ethylenediaminetetraacetate, boric acid, manganese sulfate, copper sulfate, zinc sulfate, sodium molybdate, and composite fermentation liquid for later use;

[0020] Calcium nitrate tetrahydrate, potassium nitrate, magnesium sulfate heptahydrate, manganese sulfate, copper sulfate, zinc sulfate, and sodium molybdate are mixed to obtain solution A; ammonium dihydrogen phosphate, boric acid, and disodium iron ethylenediaminetetraacetate are mixed to obtain solution B;

[0021] The solution A, the solution B and the composite fermentation liquid are mixed to obtain the hydroponic nutrient solution.

[0022] The invention also provides a use of the hydroponic nutrient solution in promoting plant growth or resisting plant diseases.

[0023] Preferably, the plant is a forage grass.

[0024] Compared with the prior art, the beneficial effects of the present invention are:

[0025] 1. The hydroponic nutrient solution provided by the present invention for cultivating forage germplasm resources, calcium nitrate tetrahydrate provides calcium ions required for growth to plants, improves the selective absorption capacity of root cell membranes, and enhances resistance to environmental stress; ammonium dihydrogen phosphate provides nitrogen elements required for growth to plants, and promotes the development of plant leaves; potassium nitrate provides potassium elements required for growth to plants, and potassium ions can also change the absorption of water and oxygen in the external environment by the root system, effectively regulate the absorption rate of oxygen in the external environment by the root system, and avoid root rot due to lack of oxygen in the root system during the growth process of the plant; disodium ferric ethylenediaminetetraacetic acid provides plants with potassium elements required for growth. Supply iron ions; magnesium sulfate provides magnesium ions and sulfate ions to plants, promoting photosynthesis of plants and promoting the synthesis of proteins under photosynthesis; manganese sulfate provides the manganese element required for plant growth, promotes photosynthesis of plants, and helps to improve the disease resistance of plants; copper sulfate provides copper elements for plants and regulates the absorption of the external environment by root cells, and zinc sulfate provides zinc elements for plants; boric acid provides boron elements required for plant growth, promotes the normal operation of carbohydrates, and can also promote the production of auxin, thereby promoting faster growth of plants; sodium molybdate provides molybdenum elements required for plant growth.

[0026] 2. The compound fermentation liquid contains nitrogen, phosphorus, potassium, iron, magnesium, copper, zinc, molybdenum and other elements needed by plants. It can help plants grow strong and luxuriantly, and can also improve the plants' resistance to diseases. In addition, the mixed fermented aloe vera juice has better antibacterial and bactericidal effects, and can effectively prevent the growth of bacteria and the rot of plant roots during the hydroponic culture of plants; at the same time, after the composite fermentation liquid is fermented, a chitosan solution is used for clarification treatment. Chitosan has an antibacterial effect and can induce the plant to have a disease resistance reaction. It works together with the aloe vera juice fermentation product in the composite fermentation liquid to better prevent the root rot during the hydroponic culture of plants; the excess chitosan in the composite fermentation liquid can form a chitosan copper chelate with the copper ions in the culture medium. This substance has a positive effect on the prevention and control of plant diseases and pests and the absorption of trace elements by plants. Moreover, since chitosan can be used as a good film-forming agent, the presence of chitosan can also form a protective film on the surface of the plant root system. While playing an antibacterial role, it can also effectively prevent pathogenic microorganisms from invading the plant root system, and does not hinder the plant's absorption of other elements in the hydroponic nutrient solution.

[0027] 3. The nitrate ions ionized from the composite fermentation liquid, potassium nitrate and nitrate ions combine with the hydrogen ions at the water ionization site to form a weakly acidic environment in the hydroponic system, thereby forming a beneficial bacterial community around the roots of the plants, controlling the invasion of harmful pathogens and effectively preventing the problem of root rot in hydroponic plants.

[0028] 4. The hydroponic nutrient solution for cultivating forage germplasm resources provided by the present invention is easy to use and only requires 2 to 3 drops to be dripped every 7 to 10 days; and the raw materials are easily available, the preparation process is simple, and the operation is convenient. DETAILED DESCRIPTION

[0029] In order to enable those skilled in the art to better understand and implement the technical solution of the present invention, the present invention is further described below in conjunction with specific embodiments, but the embodiments are not intended to limit the present invention.

[0030] The calcium nitrate tetrahydrate, potassium nitrate, ammonium dihydrogen phosphate, magnesium sulfate heptahydrate, ethylenediaminetetraacetic acid disodium iron, boric acid, manganese sulfate, copper sulfate, zinc sulfate, rice, millet, and aloe used in the embodiments of the present invention are all commercially available, and the test methods, unless otherwise specified, are all conventional methods.

[0031] The plant lactobacillus and Bacillus thuringiensis used in the embodiments of the present invention were purchased from Hubei Industrial Microbiological Culture Collection and Research Center, with the collection number of plant lactobacillus being HBCICC56402 and the collection number of Bacillus thuringiensis being HBCICC 80115. The candidum fungus used was purchased from China General Microbiological Culture Collection Administration Center with the collection number of CGMCCNo 2.1327.

[0032] The aloe juice described in the present invention is freshly squeezed juice of aloe.

[0033] Example 1

[0034] A hydroponic nutrient solution for cultivating forage germplasm resources, which is made of the following components: 372 mg of calcium nitrate tetrahydrate, 314 mg of potassium nitrate, 73 mg of ammonium dihydrogen phosphate, 262 mg of magnesium sulfate heptahydrate, 45.8 mg of disodium iron ethylenediaminetetraacetate, 2.55 mg of boric acid, 2.06 mg of manganese sulfate, 0.09 mg of copper sulfate, 0.32 mg of zinc sulfate, 0.02 mg of sodium molybdate, and 305 mL of composite fermentation liquid;

[0035] The composite fermentation broth is prepared according to the following steps:

[0036] 100 g of rice washing water and 80 g of aloe vera juice were mixed, and a composite bacterial agent was added at an inoculum rate of 0.8%, and the mixture was fermented at 35°C for 30 h. Chitosan equivalent to 1.2% of its total weight was added to the obtained fermentation liquid. After clarification for 6 h, the mixture was centrifuged at 1000 r / min for 8 min, and the supernatant was collected. The composite bacterial agent was composed of Lactobacillus plantarum, Geotrichum candidum and Bacillus thuringiensis in equal proportions, and the number of effective live bacteria in the composite bacterial agent was 5×10 9 cfu / mL.

[0037] The rice washing water is obtained by adding water equal to 8 times the mass of rice to the rice and soaking it for 0.5 hours, and then filtering the filtrate.

[0038] The method for preparing the hydroponic nutrient solution for cultivating the forage germplasm resources is specifically carried out according to the following steps:

[0039] S1. Weigh calcium nitrate tetrahydrate, potassium nitrate, ammonium dihydrogen phosphate, magnesium sulfate heptahydrate, disodium ferric ethylenediaminetetraacetate, boric acid, manganese sulfate, copper sulfate, zinc sulfate, sodium molybdate, and composite fermentation liquid for later use;

[0040] S2, mixing the calcium nitrate tetrahydrate, potassium nitrate, magnesium sulfate heptahydrate, manganese sulfate, copper sulfate, zinc sulfate, and sodium molybdate in S1 to obtain solution A;

[0041] The ammonium dihydrogen phosphate, boric acid and disodium ferric ethylenediaminetetraacetate in S1 are mixed to obtain solution B;

[0042] S3. Mix solution A, solution B and the composite fermentation liquid, add deionized water to make the volume to 1 L, and obtain the hydroponic nutrient solution.

[0043] When using hydroponic nutrient solution, dilute it 10 times and add 2 to 3 drops every 7 to 10 days.

[0044] Example 2

[0045] A hydroponic nutrient solution for cultivating forage germplasm resources, which is made of the following components: 417 mg of calcium nitrate tetrahydrate, 328 mg of potassium nitrate, 89 mg of ammonium dihydrogen phosphate, 283 mg of magnesium sulfate heptahydrate, 57.4 mg of disodium iron ethylenediaminetetraacetate, 2.71 mg of boric acid, 2.14 mg of manganese sulfate, 0.11 mg of copper sulfate, 0.37 mg of zinc sulfate, 0.025 mg of sodium molybdate, and 450 mL of composite fermentation liquid;

[0046] The above-mentioned composite fermentation broth is prepared according to the following steps:

[0047] 180g of rice washing water and 120g of aloe vera juice were mixed, and a composite bacterial agent was added according to an inoculum amount of 1.2wt%, and fermented at 37°C for 32h. The resulting fermentation liquid was added with chitosan equivalent to 2.5% of its total weight. After clarifying for 8h, the mixture was centrifuged at 1000r / min for 8min, and the supernatant was collected. The composite bacterial agent was composed of Lactobacillus plantarum, Geotrichum candidum and Bacillus thuringiensis in equal proportions, and the number of effective live bacteria in the composite bacterial agent was 8×10 9 cfu / mL.

[0048] The rice washing water is obtained by adding water equal to 8 times the mass of rice to the rice and soaking it for 0.5 hours, and then filtering the filtrate.

[0049] The method for preparing the hydroponic nutrient solution for cultivating the forage germplasm resources is specifically carried out in the following steps:

[0050] S1. Weigh calcium nitrate tetrahydrate, potassium nitrate, ammonium dihydrogen phosphate, magnesium sulfate heptahydrate, disodium ferric ethylenediaminetetraacetate, boric acid, manganese sulfate, copper sulfate, zinc sulfate, sodium molybdate, and composite fermentation liquid for later use;

[0051] S2, mixing the calcium nitrate tetrahydrate, potassium nitrate, magnesium sulfate heptahydrate, manganese sulfate, copper sulfate, zinc sulfate, and sodium molybdate in S1 to obtain solution A;

[0052] The ammonium dihydrogen phosphate, boric acid and disodium ferric ethylenediaminetetraacetate in S1 are mixed to obtain solution B;

[0053] S3. Mix solution A, solution B and the composite fermentation liquid, add deionized water to make the volume to 1 L, and obtain the hydroponic nutrient solution.

[0054] When using hydroponic nutrient solution, dilute it 12 times and add 2 to 3 drops every 7 to 10 days.

[0055] Example 3

[0056] A hydroponic nutrient solution for cultivating forage germplasm resources, which is made of the following components: 4.3 mg / L calcium nitrate tetrahydrate, 349 mg potassium nitrate, 95 mg ammonium dihydrogen phosphate, 283 mg magnesium sulfate heptahydrate, 62.1 mg disodium iron ethylenediaminetetraacetate, 2.67 mg boric acid, 2.11 mg manganese sulfate, 0.12 mg copper sulfate, 0.44 mg zinc sulfate, 0.03 mg sodium molybdate, and 416 mL composite fermentation liquid;

[0057] The composite fermentation liquid is prepared by mixing 200 g of rice washing water and 120 g of aloe juice, adding a composite bacterial agent according to an inoculum amount of 2 wt%, fermenting at 32° C. for 36 h, adding chitosan equivalent to 1.5% of its total weight to the obtained fermentation liquid, clarifying for 10 h, centrifuging at 1000 r / min for 10 min, and collecting the supernatant; the composite bacterial agent is composed of Lactobacillus plantarum, Geotrichum candidum and Bacillus thuringiensis in equal proportions, and the number of effective live bacteria in the composite bacterial agent is 6×10 9 cfu / mL.

[0058] Among them, rice washing water is the filtrate obtained by adding water equivalent to 10 times the mass of rice to soak for 1 hour and filtering it.

[0059] The method for preparing the hydroponic nutrient solution for cultivating the forage germplasm resources is specifically carried out in the following steps:

[0060] S1. Weigh calcium nitrate tetrahydrate, potassium nitrate, ammonium dihydrogen phosphate, magnesium sulfate heptahydrate, disodium ferric ethylenediaminetetraacetate, boric acid, manganese sulfate, copper sulfate, zinc sulfate, sodium molybdate, and composite fermentation liquid for later use;

[0061] S2, mixing the calcium nitrate tetrahydrate, potassium nitrate, magnesium sulfate heptahydrate, manganese sulfate, copper sulfate, zinc sulfate, and sodium molybdate in S1 to obtain solution A;

[0062] The ammonium dihydrogen phosphate, boric acid and disodium ferric ethylenediaminetetraacetate in S1 are mixed to obtain solution B;

[0063] S3. Mix solution A, solution B and the composite fermentation liquid, add deionized water to make the volume to 1 L, and obtain the hydroponic nutrient solution.

[0064] When using hydroponic nutrient solution, dilute it 12 times and add 2 to 3 drops every 7 to 10 days.

[0065] Example 4

[0066] A hydroponic nutrient solution for cultivating forage germplasm resources, which is made of the following components: 372 mg of calcium nitrate tetrahydrate, 334 mg of potassium nitrate, 102 mg of ammonium dihydrogen phosphate, 269 mg of magnesium sulfate heptahydrate, 62.1 mg of disodium ferric ethylenediaminetetraacetate, 2.57 mg of boric acid, 2.08 mg of manganese sulfate, 0.13 mg of copper sulfate, 0.37 mg of zinc sulfate, 0.02 mg of sodium molybdate, and 450 mL of composite fermentation liquid;

[0067] The composite fermentation liquid is prepared by mixing 80g of rice washing water and 120g of aloe juice, adding a composite bacterial agent according to an inoculum amount of 2wt%, fermenting at 40°C for 28h, adding 2.0% chitosan to the obtained fermentation liquid, clarifying for 10h, centrifuging at 1000r / min for 10min, and collecting the supernatant; the composite bacterial agent is composed of Lactobacillus plantarum, Geotrichum candidum and Bacillus thuringiensis in a mass ratio of 1:1:2, and the number of effective live bacteria in the composite bacterial agent is 6×10 9 cfu / mL.

[0068] Among them, rice washing water is the filtrate obtained by adding water equivalent to 10 times the mass of rice to soak for 1 hour and filtering it.

[0069] The method for preparing the hydroponic nutrient solution for cultivating the forage germplasm resources is specifically carried out in the following steps:

[0070] S1. Weigh calcium nitrate tetrahydrate, potassium nitrate, ammonium dihydrogen phosphate, magnesium sulfate heptahydrate, disodium ferric ethylenediaminetetraacetate, boric acid, manganese sulfate, copper sulfate, zinc sulfate, sodium molybdate, and composite fermentation liquid for later use;

[0071] S2, mixing the calcium nitrate tetrahydrate, potassium nitrate, magnesium sulfate heptahydrate, manganese sulfate, copper sulfate, zinc sulfate, and sodium molybdate in S1 to obtain solution A;

[0072] The ammonium dihydrogen phosphate, boric acid and disodium ferric ethylenediaminetetraacetate in S1 are mixed to obtain solution B;

[0073] S3. Mix solution A, solution B and the composite fermentation liquid, add deionized water to make the volume to 1 L, and obtain the hydroponic nutrient solution.

[0074] When using hydroponic nutrient solution, dilute it 12 times and add 2 to 3 drops every 7 to 10 days.

[0075] Example 5

[0076] A hydroponic nutrient solution for cultivating forage germplasm resources, which is made of the following components: 384 mg of calcium nitrate tetrahydrate, 335 mg of potassium nitrate, 87 mg of ammonium dihydrogen phosphate, 274 mg of magnesium sulfate heptahydrate, 51.8 mg of disodium iron ethylenediaminetetraacetate, 2.63 mg of boric acid, 2.06 mg of manganese sulfate, 0.11 mg of copper sulfate, 0.38 mg of zinc sulfate, 0.02 mg of sodium molybdate, and 420 mL of composite fermentation liquid;

[0077] The composite fermentation liquid is prepared by mixing 100 g of rice washing water and 120 g of aloe juice, adding a composite bacterial agent at an inoculation rate of 2 wt%, fermenting at 40° C. for 30 h, adding chitosan equivalent to 1 wt % of the total weight of the obtained fermentation liquid, clarifying for 10 h, centrifuging at 1000 r / min for 10 min, and collecting the supernatant; the composite bacterial agent is composed of plant lactobacillus, candidum and Bacillus thuringiensis in a mass ratio of 2:1:1, and the number of effective live bacteria in the composite bacterial agent is 6×10 9 cfu / mL.

[0078] Among them, rice washing water is the filtrate obtained by adding water equivalent to 10 times the mass of rice to soak for 1 hour and filtering it.

[0079] The method for preparing the hydroponic nutrient solution for cultivating the forage germplasm resources is specifically carried out in the following steps:

[0080] S1. Weigh calcium nitrate tetrahydrate, potassium nitrate, ammonium dihydrogen phosphate, magnesium sulfate heptahydrate, disodium ferric ethylenediaminetetraacetate, boric acid, manganese sulfate, copper sulfate, zinc sulfate, sodium molybdate, and composite fermentation liquid for later use;

[0081] S2, mixing the calcium nitrate tetrahydrate, potassium nitrate, magnesium sulfate heptahydrate, manganese sulfate, copper sulfate, zinc sulfate, and sodium molybdate in S1 to obtain solution A;

[0082] The ammonium dihydrogen phosphate, boric acid and disodium ferric ethylenediaminetetraacetate in S1 are mixed to obtain solution B;

[0083] S3. Mix solution A, solution B and the composite fermentation liquid, add deionized water to make the volume to 1 L, and obtain the hydroponic nutrient solution.

[0084] When using hydroponic nutrient solution, dilute it 12 times and add 2 to 3 drops every 7 to 10 days.

[0085] Example 6

[0086] A hydroponic nutrient solution for cultivating forage germplasm resources, which is made of the following components: 417 mg of calcium nitrate tetrahydrate, 349 mg of potassium nitrate, 102 mg of ammonium dihydrogen phosphate, 283 mg of magnesium sulfate heptahydrate, 62.1 mg of disodium ferric ethylenediaminetetraacetate, 2.71 mg of boric acid, 2.14 mg of manganese sulfate, 0.13 mg of copper sulfate, 0.44 mg of zinc sulfate, 0.03 mg of sodium molybdate, and 450 mL of composite fermentation liquid;

[0087] The composite fermentation liquid is prepared by mixing 100 g of rice washing water and 120 g of aloe juice, adding a composite bacterial agent at an inoculation rate of 2 wt%, fermenting at 40° C. for 30 h, adding chitosan equivalent to 1.5% of its total weight to the obtained fermentation liquid, clarifying for 10 h, centrifuging at 1000 r / min for 10 min, and collecting the supernatant; the composite bacterial agent is composed of Lactobacillus plantarum, Geotrichum candidum and Bacillus thuringiensis in equal proportions, and the number of effective live bacteria in the composite bacterial agent is 6×10 9 cfu / mL.

[0088] Among them, rice washing water is the filtrate obtained by adding water equivalent to 10 times the mass of rice to soak for 1 hour and filtering it.

[0089] The method for preparing the hydroponic nutrient solution for cultivating the forage germplasm resources is specifically carried out in the following steps:

[0090] S1. Weigh calcium nitrate tetrahydrate, potassium nitrate, ammonium dihydrogen phosphate, magnesium sulfate heptahydrate, disodium ferric ethylenediaminetetraacetate, boric acid, manganese sulfate, copper sulfate, zinc sulfate, sodium molybdate, and composite fermentation liquid for later use;

[0091] S2, mixing the calcium nitrate tetrahydrate, potassium nitrate, magnesium sulfate heptahydrate, manganese sulfate, copper sulfate, zinc sulfate, and sodium molybdate in S1 to obtain solution A;

[0092] The ammonium dihydrogen phosphate, boric acid and disodium ferric ethylenediaminetetraacetate in S1 are mixed to obtain solution B;

[0093] S3. Mix solution A, solution B and the composite fermentation liquid, add deionized water to make the volume to 1 L, and obtain the hydroponic nutrient solution.

[0094] When using hydroponic nutrient solution, dilute it 15 times and add 2 to 3 drops every 7 to 10 days.

[0095] Comparative Example 1

[0096] A hydroponic nutrient solution for cultivating forage germplasm resources, which is made of the following components: 384 mg of calcium nitrate tetrahydrate, 335 mg of potassium nitrate, 87 mg of ammonium dihydrogen phosphate, 274 mg of magnesium sulfate heptahydrate, 51.8 mg of disodium ferric ethylenediaminetetraacetate, 2.63 mg of boric acid, 2.06 mg of manganese sulfate, 0.11 mg of copper sulfate, 0.38 mg of zinc sulfate, and 0.02 mg of sodium molybdate;

[0097] The method for preparing the hydroponic nutrient solution for cultivating the forage germplasm resources is specifically carried out in the following steps:

[0098] S1. Weigh calcium nitrate tetrahydrate, potassium nitrate, ammonium dihydrogen phosphate, magnesium sulfate heptahydrate, disodium ferric ethylenediaminetetraacetate, boric acid, manganese sulfate, copper sulfate, zinc sulfate, and sodium molybdate for later use;

[0099] S2, mixing the calcium nitrate tetrahydrate, potassium nitrate, magnesium sulfate heptahydrate, manganese sulfate, copper sulfate, zinc sulfate, and sodium molybdate in S1 to obtain solution A;

[0100] The ammonium dihydrogen phosphate, boric acid and disodium ferric ethylenediaminetetraacetate in S1 are mixed to obtain solution B;

[0101] S3. Mix solution A and solution B, add deionized water to make the volume to 1 L, and obtain the hydroponic nutrient solution.

[0102] When using hydroponic nutrient solution, dilute it 10 times and add 2 to 3 drops every 7 to 10 days.

[0103] Comparative Example 2

[0104] A hydroponic nutrient solution for cultivating forage germplasm resources, which is made of the following components: 384 mg of calcium nitrate tetrahydrate, 335 mg of potassium nitrate, 87 mg of ammonium dihydrogen phosphate, 274 mg of magnesium sulfate heptahydrate, 51.8 mg of disodium iron ethylenediaminetetraacetate, 2.63 mg of boric acid, 2.06 mg of manganese sulfate, 0.11 mg of copper sulfate, 0.38 mg of zinc sulfate, 0.02 mg of sodium molybdate, and 420 mL of composite fermentation liquid;

[0105] The composite fermentation liquid is prepared by mixing 100 g of rice washing water and 120 g of aloe vera juice, adding a composite bacterial agent at an inoculum amount of 2 wt % and fermenting at 40° C. for 30 h. The obtained fermentation liquid is centrifuged at 1000 r / min for 10 min, and the supernatant is collected. The composite bacterial agent is composed of Lactobacillus plantarum, Geotrichum candidum and Bacillus thuringiensis in equal proportions, and the number of effective live bacteria in the composite bacterial agent is 6×10 9 cfu / mL.

[0106] Among them, rice washing water is the filtrate obtained by adding water equivalent to 10 times the mass of rice to soak for 1 hour and filtering it.

[0107] The method for preparing the hydroponic nutrient solution for cultivating the forage germplasm resources is specifically carried out in the following steps:

[0108] S1. Weigh calcium nitrate tetrahydrate, potassium nitrate, ammonium dihydrogen phosphate, magnesium sulfate heptahydrate, disodium ferric ethylenediaminetetraacetate, boric acid, manganese sulfate, copper sulfate, zinc sulfate, sodium molybdate, and composite fermentation liquid for later use;

[0109] S2, mixing the calcium nitrate tetrahydrate, potassium nitrate, magnesium sulfate heptahydrate, manganese sulfate, copper sulfate, zinc sulfate, and sodium molybdate in S1 to obtain solution A;

[0110] The ammonium dihydrogen phosphate, boric acid and disodium ferric ethylenediaminetetraacetate in S1 are mixed to obtain solution B;

[0111] S3. Mix solution A, solution B and the composite fermentation liquid, add deionized water to make the volume to 1 L, and obtain the hydroponic nutrient solution.

[0112] When using hydroponic nutrient solution, dilute it 12 times and add 2 to 3 drops every 7 to 10 days.

[0113] Comparative Example 3

[0114] A hydroponic nutrient solution for cultivating forage germplasm resources, which is made of the following components: 384 mg of calcium nitrate tetrahydrate, 335 mg of potassium nitrate, 87 mg of ammonium dihydrogen phosphate, 274 mg of magnesium sulfate heptahydrate, 51.8 mg of disodium iron ethylenediaminetetraacetate, 2.63 mg of boric acid, 2.06 mg of manganese sulfate, 0.11 mg of copper sulfate, 0.38 mg of zinc sulfate, 0.02 mg of sodium molybdate, and 420 mL of composite fermentation liquid;

[0115] The composite fermentation liquid is prepared by adding a composite bacterial agent at an inoculum amount of 2 wt % to 100 g of rice washing water, fermenting at 40° C. for 30 h, adding chitosan equivalent to 1 wt % of the total weight to the obtained fermentation liquid, clarifying for 10 h, centrifuging at 1000 r / min for 10 min, and collecting the supernatant; the composite bacterial agent is composed of Lactobacillus plantarum, Geotrichum candidum and Bacillus thuringiensis in equal proportions, and the number of effective live bacteria in the composite bacterial agent is 6×10 9 cfu / mL.

[0116] Among them, rice washing water is the filtrate obtained by adding water equivalent to 10 times the mass of rice to soak for 1 hour and filtering it.

[0117] The method for preparing the hydroponic nutrient solution for cultivating the forage germplasm resources is specifically carried out in the following steps:

[0118] S1. Weigh calcium nitrate tetrahydrate, potassium nitrate, ammonium dihydrogen phosphate, magnesium sulfate heptahydrate, disodium ferric ethylenediaminetetraacetate, boric acid, manganese sulfate, copper sulfate, zinc sulfate, sodium molybdate, and composite fermentation liquid for later use;

[0119] S2, mixing the calcium nitrate tetrahydrate, potassium nitrate, magnesium sulfate heptahydrate, manganese sulfate, copper sulfate, zinc sulfate, and sodium molybdate in S1 to obtain solution A;

[0120] The ammonium dihydrogen phosphate, boric acid and disodium ferric ethylenediaminetetraacetate in S1 are mixed to obtain solution B;

[0121] S3. Mix solution A, solution B and the composite fermentation liquid, add deionized water to make the volume to 1 L, and obtain the hydroponic nutrient solution.

[0122] When using hydroponic nutrient solution, dilute it 12 times and add 2 to 3 drops every 7 to 10 days.

[0123] Comparative Example 4

[0124] A hydroponic nutrient solution for cultivating forage germplasm resources, which is made of the following components: 384 mg of calcium nitrate tetrahydrate, 335 mg of potassium nitrate, 87 mg of ammonium dihydrogen phosphate, 274 mg of magnesium sulfate heptahydrate, 51.8 mg of disodium iron ethylenediaminetetraacetate, 2.63 mg of boric acid, 2.06 mg of manganese sulfate, 0.11 mg of copper sulfate, 0.38 mg of zinc sulfate, 0.02 mg of sodium molybdate, and 420 mL of composite fermentation liquid;

[0125] The composite fermentation liquid is prepared by mixing 100 g of rice washing water and 120 g of aloe vera juice, adding Bacillus thuringiensis at an inoculum amount of 2 wt%, fermenting at 40°C for 30 h, adding chitosan equivalent to 1.0% of its total weight to the obtained fermentation liquid, clarifying for 10 h, centrifuging at 1000 r / min for 10 min, and collecting the supernatant; the effective viable count of Bacillus thuringiensis is 5×10 9 cfu / mL.

[0126] Among them, rice washing water is the filtrate obtained by adding water equivalent to 10 times the mass of rice to soak for 1 hour and filtering it.

[0127] The method for preparing the hydroponic nutrient solution for cultivating the forage germplasm resources is specifically carried out in the following steps:

[0128] S1. Weigh calcium nitrate tetrahydrate, potassium nitrate, ammonium dihydrogen phosphate, magnesium sulfate heptahydrate, disodium ferric ethylenediaminetetraacetate, boric acid, manganese sulfate, copper sulfate, zinc sulfate, sodium molybdate, and composite fermentation liquid for later use;

[0129] S2, mixing the calcium nitrate tetrahydrate, potassium nitrate, magnesium sulfate heptahydrate, manganese sulfate, copper sulfate, zinc sulfate, and sodium molybdate in S1 to obtain solution A;

[0130] The ammonium dihydrogen phosphate, boric acid and disodium ferric ethylenediaminetetraacetate in S1 are mixed to obtain solution B;

[0131] S3. Mix solution A, solution B and the composite fermentation liquid, add deionized water to make the volume to 1 L, and obtain the hydroponic nutrient solution.

[0132] When using hydroponic nutrient solution, dilute it 12 times and add 2 to 3 drops every 7 to 10 days.

[0133] Examples 1 to 6 all prepared hydroponic nutrient solutions with good effects. Since the hydroponic nutrient solutions prepared in Examples 1 to 6 have basically the same effects, the following only takes the nutrient solution prepared in Example 5 as an example to compare with various comparative examples to illustrate the effects of the present invention.

[0134] The culture solution prepared in Example 5 and the culture solutions prepared in Comparative Examples 1 to 4 were diluted by corresponding multiples and used for seedling cultivation of wheatgrass, oats, and ryegrass, respectively. Two drops were added every 7 days from the time when the wheatgrass, oats, and ryegrass turned white. After 4 times, the root growth and growth status of the plants, the status of the hydroponic substrate, and the root rot of the plants were counted. The results are shown in Tables 1 to 3.

[0135] Table 1 Effects on wheatgrass cultivation

[0136]

[0137] Table 2 Effects on oat cultivation

[0138]

[0139]

[0140] Table 3 Effects on ryegrass cultivation

[0141]

[0142] Note: In the above Tables 1 to 3, the degree of odor is: obvious odor > slight odor > slight odor > slight odor > no odor. The degree of root rot is: root rot > slight root rot > slight root rot > no root rot.

[0143] It can be seen from the above results that the hydroponic nutrient solution provided by the present invention is suitable for hydroponic cultivation of various forage grasses, and while promoting plant growth, it can also resist infection by pathogenic microorganisms and effectively prevent root rot of plants during hydroponic cultivation.

[0144] It should be noted that when the claims of the present invention involve a numerical range, it should be understood that the two endpoints of each numerical range and any value between the two endpoints can be selected. Since the steps and methods used are the same as those in Examples 1 to 3, in order to avoid redundancy, the present invention describes the preferred embodiments, but once those skilled in the art know the basic creative concept, they can make other changes and modifications to these embodiments. Therefore, the attached claims are intended to be interpreted as including the preferred embodiments and all changes and modifications that fall within the scope of the present invention.

[0145] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A hydroponic nutrient solution for cultivating forage germplasm resources, characterized in that: It is made of the following components: 372-417 mg / L of calcium nitrate tetrahydrate, 314-349 mg / L of potassium nitrate, 73-102 mg / L of ammonium dihydrogen phosphate, 262-283 mg / L of magnesium sulfate heptahydrate, 45.8-62.1 mg / L of disodium ferric ethylenediaminetetraacetate, 2.55-2.71 mg / L of boric acid, 2.06-2.14 mg / L of manganese sulfate, 0.09-0.13 mg / L of copper sulfate, 0.32-0.44 mg / L of zinc sulfate, 0.02-0.03 mg / L of sodium molybdate, and 305-450 mL / L of composite fermentation liquid; The composite fermentation broth is prepared according to the following steps: 10 to 20 parts of rice washing water and 8 to 12 parts of aloe vera juice are mixed, and a composite bacterial agent is added at an inoculum amount of 0.8 to 2 wt % for fermentation, wherein the composite bacterial agent is composed of Lactobacillus plantarum, Geotrichum candidum and Bacillus thuringiensis; Add chitosan to the obtained fermentation liquid, let it stand, and collect the supernatant to obtain the composite fermentation liquid; The rice washing water is the filtrate obtained by adding water equivalent to 8 to 10 times the total mass of rice or millet or a mixture of the two and soaking for 0.5 to 1 hour.

2. The hydroponic nutrient solution according to claim 1, characterized in that: It is made of the following components: 384 mg / L calcium nitrate tetrahydrate, 335 mg / L potassium nitrate, 87 mg / L ammonium dihydrogen phosphate, 274 mg / L magnesium sulfate heptahydrate, 51.8 mg / L ethylenediaminetetraacetic acid disodium iron, 2.63 mg / L boric acid, 2.06 mg / L manganese sulfate, 0.11 mg / L copper sulfate, 0.38 mg / L zinc sulfate, 0.02 mg / L sodium molybdate, and 420 mL / L composite fermentation liquid; The composite fermentation liquid is prepared by mixing 18 parts of rice washing water and 10 parts of aloe juice, and adding a composite bacterial agent at an inoculation amount of 1.5 wt % for fermentation.

3. The hydroponic nutrient solution according to claim 1, characterized in that: The amount of chitosan added is 1.0-2.5% based on the total mass of the fermentation liquid.

4. The hydroponic nutrient solution according to claim 1, characterized in that: The composite bacterial agent is composed of Lactobacillus plantarum, Geotrichum candidum and Bacillus thuringiensis in equal proportions, and the number of effective live bacteria in the composite bacterial agent is 5 to 8×10 9 cfu / mL.

5. The hydroponic nutrient solution according to claim 1, characterized in that: The fermentation is carried out at 32-40° C. for 28-36 hours.

6. The hydroponic nutrient solution according to claim 1, characterized in that: The standing is standing at 10-25° C. for 6-10 hours.

7. A method for preparing the hydroponic nutrient solution according to claim 1, characterized in that: Follow these steps: Weigh calcium nitrate tetrahydrate, potassium nitrate, ammonium dihydrogen phosphate, magnesium sulfate heptahydrate, disodium ferric ethylenediaminetetraacetate, boric acid, manganese sulfate, copper sulfate, zinc sulfate, sodium molybdate, and composite fermentation liquid for later use; Calcium nitrate tetrahydrate, potassium nitrate, magnesium sulfate heptahydrate, manganese sulfate, copper sulfate, zinc sulfate, and sodium molybdate are mixed to obtain solution A; ammonium dihydrogen phosphate, boric acid, and disodium iron ethylenediaminetetraacetate are mixed to obtain solution B; The solution A, the solution B and the composite fermentation liquid are mixed to obtain the hydroponic nutrient solution.

8. Use of the hydroponic nutrient solution according to claim 1 in promoting plant growth or resisting plant diseases.

9. The use according to claim 8, characterized in that The plant is forage grass.

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