A bactericidal composition comprising methamidophos and fluopyram and its application.

Abstract

This invention belongs to the field of agricultural fungicides, and provides a fungicidal composition containing methamidophos and fluopyram and its application, wherein the mass ratio of methamidophos and fluopyram is 1:2-1:70; the fungicidal composition has excellent synergistic effects on crop diseases; it can effectively reduce the dosage of pesticides, slow down the development of pesticide resistance in diseases, reduce environmental pollution, and is safe for crops in field observations.

Description

Technical Field

[0001] This invention belongs to the field of agricultural fungicides, specifically relating to a fungicidal composition comprising methamidophos and fluopyram and its application. Background Technology

[0002] Methiazolidinone is a thiadiazole-based plant disease activator jointly developed by Nankai University. CN100420686C discloses the synthesis method of methiazolidinone and also discloses its excellent control effects against *Fusarium wilt* of cucumber, *Fusarium graminearum* of wheat, *Aspergillus oryzae* of rice, *Sclerotinia sclerotinia* of rapeseed, *Fungi rot*, *Anthracnose* of banana, *Anthracnose* of cucumber, *Verticillium wilt* of cotton, and late blight of potato. CN101091490A discloses its excellent control effect against downy mildew of cucumber. Current research results show that this compound has a good inducing effect on viral diseases, and its mechanism of action is to induce systemic and broad-spectrum resistance activity in plants.

[0003] Fluopyram is a pyrazole carboxamide fungicide with a mechanism of action as a succinate dehydrogenase inhibitor (SDHI). Fluopyram can quickly bind to the waxy layer, resist rain washout, and increase its residual effect. It can control wheat scab, rapeseed sclerotinia rot, and peanut leaf spot.

[0004] The current unregulated use of pesticides has led to increasingly stronger resistance from diseases, resulting in a year-on-year increase in pesticide usage and consequently, more severe environmental pollution. Therefore, the rational use of pesticides and the preparation of fungicide combinations to broaden the fungicidal spectrum and reduce disease resistance are urgently needed. There are currently no reports on the combination of methamidophos and fluopyram. Summary of the Invention

[0005] This invention provides a bactericidal composition with excellent synergistic effects, the specific technical solution of which is as follows:

[0006] A bactericidal composition comprising methamidophos and fluopyram, wherein the active ingredients are methamidophos and fluopyram, and the mass ratio of methamidophos to fluopyram is 1:2 to 1:70.

[0007] The mass ratio of methamidophos and fluopyram in the bactericidal composition is 1:5-1:50.

[0008] The mass ratio of methamidophos and fluopyram in the bactericidal composition is 1:10-1:40.

[0009] The mass ratio of methamidophos and fluopyram in the bactericidal composition is 1:10-1:25.

[0010] The bactericidal composition contains adjuvants, and the weight percentage of the active ingredient is 1-70%. Preferably, the weight percentage of the active ingredient is 10-50%.

[0011] The additives are one or more of the following: wetting agents, emulsifiers, dispersants, suspending agents, antifreeze agents, preservatives, thickeners, defoamers, solvents, and fillers.

[0012] The bactericidal composition is formulated as a dispersible oil suspension, an aqueous suspension, a wettable powder, a water-dispersible granule, or a microemulsion.

[0013] The bactericidal composition is used for the prevention and control of crop diseases, preferably wheat scab.

[0014] By adopting the above technical solution, the present invention has the following beneficial effects:

[0015] When the mass ratio of methamidophos to fluopyram is 1:5-1:50, the composition exhibits a significant synergistic effect against wheat scab pathogens. This synergistic effect effectively reduces pesticide dosage, slows the development of pesticide resistance, and mitigates environmental pollution. Furthermore, field observations have shown its safety to crops. Detailed Implementation

[0016] The present invention will be further explained below with reference to specific embodiments.

[0017] Example 1: Indoor toxicity test of the combination of methamidophos and fluopyram to the pathogen of wheat scab.

[0018] Experimental materials:

[0019] The pathogen of wheat scab (Fusarium graminearum); collected and identified in the field, and cultured and preserved indoors.

[0020] Test method:

[0021] The mycelial growth rate was determined using the mycelial growth rate method. Based on preliminary experiments, a series of drug-containing PDA mediums with varying concentrations were prepared. Mycelial blocks were cut along the edge of the colony using a 5mm diameter punch and placed on different concentration gradients of drug-containing medium. The media were then incubated. When the control group colonies reached 4 / 5 of the plate's capacity, the colony diameter was measured using a cross-sectional method, and the mycelial growth inhibition rate was calculated. Regression analysis was performed based on the concentrations of each single agent and combination, and their corresponding efficacy, to determine the EC50 of each agent. 50 value.

[0022] Inhibition rate % = (Coronary diameter of control group - Colony diameter of treatment group) / (Coronary diameter of control group - 5) × 100%.

[0023] Using the co-toxicity coefficient method calculated by Sun Yunpei, the co-toxicity coefficient (CTC) of the mixture was calculated to determine the synergistic effect of the mixture.

[0024] Single-dose toxicity index = (EC50 of standard dose) 50 / A single-dose EC 50 )×100;

[0025] Theoretical toxicity index (TTI) = toxicity index of single agent A × proportion of single agent A in the mixture + toxicity index of single agent B × proportion of single agent B in the mixture;

[0026] Actual Toxicity Index (ATI) = (EC50 of a standard single dose) 50 EC values / mixtures 50 (value) × 100;

[0027] Cotoxicity coefficient (CTC) = (Measured toxicity index / Theoretical toxicity index) × 100;

[0028] When CTC is greater than 120, the mixture has a synergistic effect; when CTC is less than 80, it has an antagonistic effect; and when CTC is between 80 and 120, it has an additive effect.

[0029] Test Results

[0030] Table 1 shows that methoxyfenozide has an EC50-95% effect on the pathogen of wheat scab. 50 The EC50 value of fluopyram was 1.7825 mg / L, indicating that fluopyram was effective against the pathogen of wheat scab. 50 The concentration of the compound was 0.1182 mg / L. When the mass ratio of methamidophos to fluopyram was 1:5-1:50, the composition showed a significant synergistic effect against the pathogen of wheat scab, with a CTC value greater than 120. When the mass ratio of methamidophos to fluopyram was 25:1-1:1, the composition showed an additive effect against the pathogen of wheat scab. When the composition exhibits a synergistic effect, it can effectively reduce the amount of active ingredient used, reduce environmental pollution, and slow down the development of drug resistance in diseases.

[0031] Table 1: Indoor testing of the combination of methamidophos and fluopyram with wheat scab pathogen

[0032]

[0033] Example 2: Field control efficacy of methamidophos combined with fluopyram for wheat scab.

[0034] Test crop disease: Wheat Fusarium head blight

[0035] Test reagent:

[0036] 26% Methiazolidinyl and Fluopyram hydroxylamine suspension, containing the following percentage ingredients: Methiazolidinyl 1%, Fluopyram hydroxylamine 25%, Tersperse 2700 6%, Calcium Dodecylbenzene Sulfonate 2%, Sodium Lignosulfonate 2%, Ethylene Glycol 5%, Magnesium Aluminum Silicate 0.8%, Sodium Benzoate 0.2%, Dimethyl Silicone Oil 0.5%, Bentonite 0.8%, Water to make up 100%;

[0037] 24% Methiazolidinyl and Fluopyram hydroxylamine suspension, containing the following percentage ingredients: Methiazolidinyl 4%, Fluopyram hydroxylamine 20%, Tersperse 2700 4%, Calcium Dodecylbenzene Sulfonate 1%, Sodium Lignosulfonate 2%, Ethylene Glycol 4%, Magnesium Aluminum Silicate 0.6%, Sodium Benzoate 0.15%, Dimethyl Silicone Oil 0.4%, Bentonite 0.8%, Water to make up 100%;

[0038] 22% Methiazolidinedion and Fluopyram wettable powder, containing the following percentage ingredients: Methiazolidinedion 2%, Fluopyram 20%, Atlox 491 34%, NNO 3%, Silica 6%, Kaolin to make up 100%.

[0039] 32% Methiazolidinedion and Fluopyram wettable powder, containing the following percentage ingredients: Methiazolidinedion 2%, Fluopyram 30%, Atlox 491 36%, NNO 4%, precipitated silica 6%, and kaolin to make up 100%.

[0040] Test method:

[0041] The pesticide was applied twice: the first application was made at the early flowering stage of wheat, and the second application was made 7 days later; the plot area was 50m². 2 Each treatment was replicated four times. Water consumption was 450 L / hm². 21 days after application, samples were taken at five points diagonally across each plot, with 100 panicles surveyed at each point. After the survey, the disease index and control efficacy for each treatment were calculated.

[0042] Survey and calculation methods: Refer to industry standard NY / T1464.15—2007.

[0043] Grading method:

[0044] Grade 0: Disease-free throughout the ear of grain.

[0045] Grade 1: The area of ​​withered ears accounts for less than 1 / 4 of the total ear area;

[0046] Grade 3: The area of ​​dead ears accounts for 1 / 4 to 1 / 2 of the total ear area;

[0047] Grade 5: The area of ​​withered ears accounts for 1 / 2-3 / 4 of the total ear area;

[0048] Grade 7: The area of ​​dead ears accounts for more than 3 / 4 of the total ear area.

[0049]

[0050]

[0051] In the formula: CK1 is the disease index after drug administration in the blank control area; PT1 is the disease index after drug administration in the drug-treated area.

[0052] Test Results

[0053] As shown in Table 2, the tested agents of this invention have excellent field control efficacy against wheat scab, with a field control efficacy of over 94%, and the crops were observed to be safe in the field.

[0054] Table 3: Field efficacy test of compound pesticides against wheat scab

[0055]

[0056]

[0057] Finally, it should be noted that the above examples are merely a few specific embodiments of the present invention. All modifications that can be directly derived or conceived by those skilled in the art from the content disclosed in this invention are considered to be within the scope of protection of this invention.

Claims

1. A fungicidal composition comprising methlimbamine, characterized in that, The active ingredients consist of methicillin and fluopyram, with a mass ratio of methicillin to fluopyram of 1:5 to 1:

50.

2. The bactericidal composition according to claim 1, characterized by The mass ratio of methamidophos and fluopyram is 1:10-1:

40.

3. The germicidal composition according to claim 1, wherein The mass ratio of methamidophos and fluopyram is 1:10-1:

25.

4. The bactericidal composition according to any one of claims 1 to 3, characterized in that, The bactericidal composition contains adjuvants, and the weight percentage of the active ingredient is 1-70%.

5. The fungicidal composition according to claim 4, characterized in that, The weight percentage of active ingredients is 10-50%.

6. The germicidal composition according to claim 4, characterized in that, The additives are one or more of the following: wetting agents, emulsifiers, dispersants, suspending agents, antifreeze agents, preservatives, thickeners, defoamers, solvents, and fillers.

7. The germicidal composition according to claim 6, characterized in that, The bactericidal composition is formulated as a dispersible oil suspension, an aqueous suspension, a wettable powder, a water-dispersible granule, or a microemulsion.

8. The use of the bactericidal composition according to any one of claims 1-7 for the prevention and control of crop diseases, characterized in that, The crop disease is wheat scab caused by Gibberella zeae. Fusarium graminearum The crop disease is wheat scab caused by Gibberella zeae.

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