A method for closed factory seedling raising of prunus triloba

Abstract

The application discloses a closed factory seedling raising method for Prunus triloba, which comprises Prunus triloba seedling raising substrate preparation and light environment regulation technology in different growth periods, determination of CO2 saturation points of Prunus triloba in each growth period under suitable temperature, humidity and light conditions, and determination of soil and Prunus triloba respiratory CO2 release rates, and preparation of a seedling raising substrate suitable for Prunus triloba growth and development; and light environment regulation treatment is carried out on Prunus triloba seeds after seedling emergence, and the light source is a red light, blue light and far red light LED lamp. The method shortens the seedling raising period of Prunus triloba, solves the seasonal limitation problem in the Prunus triloba seedling raising process, effectively guarantees the production quality of seedlings, and provides an efficient, high-quality and energy-saving closed plant factory seedling raising technology for large-scale supply of Prunus triloba landscape afforestation seedlings.

Description

Technical Field

[0001] This invention relates to the field of industrialized seedling production technology for garden seedlings, specifically to a method for industrialized seedling production of closed-system Prunus triloba. Background Technology

[0002] *Amygdalus triloba* Lindl., also known as the 'Little Peach', belongs to the Rosaceae family and the *Prunus* genus. It is a common landscaping tree species in Northeast China and a relatively common early spring flowering plant. *Amygdalus triloba* has a long flowering period, dense foliage, and abundant, brightly colored flowers, making it popular in landscaping, urban greening, and village greening, especially in northern regions. This indicates a high demand for *Amygdalus triloba* for landscaping. However, under natural conditions, its sowing time is limited by the season, and seed germination and seedling development are slow, resulting in a long seedling period.

[0003] Plant factories utilize high-precision environmental control systems to automatically control temperature, humidity, light, CO2 concentration, and water and fertilizer conditions for plant growth. This minimizes or eliminates natural constraints on plant growth, increasing seedling production capacity. Furthermore, the three-dimensional cultivation model within plant factories maximizes the use of vertical space, thereby improving land utilization. In addition, the respiration of specific seedling substrates supplements CO2 for the photosynthesis of the elm-leaf plum, while specific ratios of red, blue, and far-red light provide suitable light quality conditions for different growth stages, maximizing energy savings, reducing seedling costs, and improving seedling efficiency.

[0004] To date, there are few reports on closed-system factory-style seedling cultivation methods for Prunus triloba. Therefore, selecting appropriate substrate ratios and seedling environment conditions is of long-term significance for the large-scale production of Prunus triloba seedlings. Summary of the Invention

[0005] In view of this, the purpose of the present invention is to provide a method for closed-loop factory-style seedling cultivation of Prunus triloba.

[0006] The specific technical solution adopted in this invention is as follows:

[0007] A method for closed-loop, factory-style seedling cultivation of Prunus triloba, characterized by the following steps:

[0008] (1) Seed germination treatment: About 7 to 15 days before sowing, soak the selected seeds in a 250 mg / L gibberellin solution for 2 to 3 days to promote germination;

[0009] (2) Determination of CO2 saturation point of Prunus triloba: CO2 gradient test was set up under suitable temperature, humidity and light conditions at each growth stage of Prunus triloba to find the CO2 saturation point of Prunus triloba at each stage;

[0010] (3) Measurement of the respiration rate of seedling substrate and elm-leaf plum: The soil respiration rate of seedling substrate and the respiration rate of elm-leaf plum at various growth stages under suitable temperature, humidity and light conditions were measured.

[0011] (4) Seedling substrate: The seedling substrate of Prunus triloba is made of organic waste materials that have been sterilized at high temperature and then combined with clay soil, water-retaining agent and stabilizer. The CO2 release rate is adjusted by adjusting the substrate ratio.

[0012] (5) Sowing and seedling raising: When 50% of the seeds of the elm-leaf plum show white sprouts, sow 2 seeds per hole in a fully moist substrate, with the sowing depth being 2-3 cm from the surface of the substrate;

[0013] (6) Seedling emergence stage: After the seedlings of *Prunus triloba* emerge from the soil, turn on the plant lights to illuminate the *Prunus triloba*. The light quality ratio of red light, blue light, and far-red light is 8:1:1, and the light intensity is 80 μmol·m⁻¹. -2 ·s -1 10 μmol·m -2 ·s -1 and 10 μmol·m -2 ·s -1 The total light intensity is 100 μmol·m -2 ·s -1 The photoperiod is 8 hours per day;

[0014] (7) Leaf area index of Prunus triloba ≥4: When the leaf area index of Prunus triloba ≥4, adjust the light quality ratio of red light, blue light and far-red light from the plant lamp to 5:1:1, and the light intensity to be 115 μmol·m⁻¹. -2 ·s -1 23 μmol·m -2 ·s -1 and 23 μmol·m -2 ·s -1 The total illuminance was 161 μmol·m. -2 ·s -1 The photoperiod is 10 hours per day;

[0015] (8) Leaf area index of Prunus triloba ≥10: When the leaf area index of Prunus triloba ≥10, adjust the light quality ratio of red light, blue light and far-red light from the plant lamp to 3:1:1, and the light intensity to be 150 μmol·m⁻¹. -2 ·s -1 50 μmol·m -2 ·s -1 and 50 μmol·m -2 ·s -1 The total light intensity is 250 μmol·m -2 ·s -1 The photoperiod is 12 hours per day;

[0016] To better realize the present invention, in step (4), the seedling substrate is adjusted in proportion so that the soil respiration rate from sowing to emergence is 1.0 to 3.0 μmol·m³. -2 ·s -1 The total amount of CO2 released daily from the seedling substrate and the respiration of the Chinese tallow tree should match the CO2 absorption by the Chinese tallow tree through photosynthesis. As the leaf area index of the Chinese tallow tree increases, the photosynthetic rate also increases. By increasing the application of nitrogen fertilizer to adjust the carbon-nitrogen ratio of the seedling substrate and adding microbial agents, the soil respiration capacity can be increased accordingly, thereby improving the CO2 release capacity in the closed plant factory environment.

[0017] To better realize the present invention, in step (4), the volume ratio of organic waste material, clay soil, water-retaining agent and stabilizer in the elm-leaf plum seedling substrate is 40-50%: 50-60%: 0.2-0.5%: 0.05-0.1%.

[0018] In order to better realize the present invention, the red light wavelength in steps (6), (7) and (8) is 640nm, the blue light wavelength is 440nm, and the far-red light wavelength is 730nm.

[0019] To better realize this invention, the ambient temperature for seedling cultivation is 25°C during the day and 15°C at night.

[0020] To better realize the present invention, in step (5), the moisture content of the seedling substrate is 40-45%.

[0021] To better realize the present invention, in steps (6), (7) and (8), the moisture content of the seedling substrate is 18-25%.

[0022] The beneficial effects of this invention are as follows:

[0023] This invention, in the closed-loop factory-style seedling cultivation of *Prunus triloba*, achieves precise control of the seedling substrate ratio and seedling environment. Firstly, the substrate ratio establishes a balance between the CO2 saturation point and the CO2 release rate from the soil and *Prunus triloba* under suitable temperature, humidity, and light conditions at various growth stages. The CO2 required for *Prunus triloba* growth is provided through soil respiration and *Prunus triloba* respiration, eliminating the need for additional supplementation. Secondly, the light quality ratio, photoperiod, and light intensity selected in this invention have significantly superior effects on the morphological characteristics and photosynthetic rate of *Prunus triloba* seedlings. Thirdly, by mounting LED beads on containerized plant growth racks, this invention allows for year-round seedling cultivation, greatly improving production efficiency while ensuring seedling quality. Finally, the precise control of the seedling substrate ratio and seedling environment in this invention is more energy-efficient and reduces costs, contributing to my country's ecological civilization construction. This invention provides a short-cycle and energy-saving method for large-scale supply of *Prunus triloba* seedlings. Detailed Implementation

[0024] The technical solution of the present invention will be further explained and illustrated below through specific embodiments.

[0025] Example

[0026] (1) Seed germination treatment: 10 days before sowing, the selected seeds are soaked in a 250 mg / L gibberellin solution for 3 days to promote germination;

[0027] (2) Determination of CO2 saturation point of Prunus triloba: During the seedling stage of Prunus triloba, the optimal air temperature, soil moisture, and light intensity were 20℃, 18%, and 100 μmol·m⁻², respectively. -2 ·s -1 The CO2 saturation point of *Prunus triloba* was measured to be 106 μmol·mol⁻¹. -1 When the leaf area index of *Prunus triloba* is ≥4, the optimal air temperature, soil moisture, and light intensity are 20℃, 20%, and 161 μmol·m⁻², respectively. -2 ·s -1 The CO2 saturation point of *Prunus triloba* was measured to be 643 μmol·mol⁻¹. -1 When the leaf area index of *Prunus triloba* is ≥10, the optimal air temperature, soil moisture, and light intensity are 20℃, 22%, and 250 μmol·m⁻², respectively. -2 ·s -1 The CO2 saturation point of *Prunus triloba* was measured to be 1607 μmol·mol⁻¹. -1 ;

[0028] (3) Measurement of respiration rate of seedling substrate and elm-leaf plum: The ratio of seedling substrate was adjusted to achieve a soil respiration rate of 1.5 μmol·m⁻¹. -2 ·s -1 During the seedling stage of *Prunus triloba*, the optimal air temperature, soil moisture, and light intensity are 25℃, 18%, and 100 μmol·m⁻², respectively. -2 ·s -1 The CO2 saturation point of *Prunus triloba* was measured to be 106 μmol·mol⁻¹. -1 The respiration rates of the seedling substrate and the elm-leaf plum were measured to be 1.2 and 0.3 μmol·m⁻¹, respectively. -2 ·s -1 When the leaf area index of *Prunus triloba* is ≥4, the optimal air temperature, soil moisture, and light intensity are 25℃, 20%, and 161 μmol·m⁻², respectively. -2 ·s -1 The respiration rates of the seedling substrate and the elm-leaf plum were measured to be 1.1 and 0.5 μmol·m⁻¹, respectively. -2 ·s -1When the leaf area index of *Prunus triloba* is ≥10, the optimal air temperature, soil moisture, and light intensity are 25℃, 22%, and 250 μmol·m⁻¹, respectively. -2 ·s -1 The respiration rates of the seedling substrate and the elm-leaf plum were measured to be 0.9 and 0.8 μmol·m⁻¹, respectively. -2 ·s -1 ;

[0029] (4) Seedling substrate: The seedling substrate of Prunus triloba is made of organic waste materials that have been sterilized at high temperature and then combined with clay soil, water-retaining agent and stabilizer. The volume ratio of organic waste materials, clay soil, water-retaining agent and stabilizer is 40.0%: 59.4%: 0.5%: 0.1%;

[0030] (5) Preparation of seedling substrate: After the clay soil is disinfected with carbendazim, it is mixed evenly according to the ratio in step (4), and watered thoroughly to make the moisture content of the seedling substrate 40%.

[0031] (6) Sowing and seedling raising: When 50% of the seeds of the elm-leaf plum show white sprouts, sow 2 seeds per hole in a fully moist substrate, with the sowing depth being 3 cm from the surface of the substrate.

[0032] (7) Seedling emergence stage: After the seedlings of *Prunus triloba* emerge from the soil, turn on the plant lights to illuminate the *Prunus triloba*. The light quality ratio of red light, blue light, and far-red light is 8:1:1, and the light intensity is 80 μmol·m⁻¹. -2 ·s -1 10 μmol·m -2 ·s -1 and 10 μmol·m -2 ·s -1 The total light intensity is 100 μmol·m -2 ·s -1 The photoperiod is 8 hours per day; the red light wavelength is 640 nm, the blue light wavelength is 440 nm, and the far-red light wavelength is 730 nm; the moisture content of the seedling substrate is 18%, and the ambient temperature for seedling cultivation is 25°C during the day and 15°C at night.

[0033] (8) Leaf area index of Prunus triloba ≥4: When the leaf area index of Prunus triloba is ≥4, adjust the light quality ratio of red light, blue light and far-red light from the plant lamp to 5:1:1, and the light intensity to be 115 μmol·m⁻¹. -2 ·s -1 23 μmol·m -2 ·s -1 and 23 μmol·m -2 ·s -1 The total illuminance was 161 μmol·m. -2 ·s -1The photoperiod is 10h / d; the red light wavelength is 640nm, the blue light wavelength is 440nm, and the far-red light wavelength is 730nm; the moisture content of the seedling substrate is 20%, and the ambient temperature for seedling cultivation is 25℃ during the day and 15℃ at night.

[0034] (9) Leaf area index of Prunus triloba ≥10: When the leaf area index of Prunus triloba ≥10, adjust the light quality ratio of red light, blue light and far-red light from the plant lamp to 3:1:1, and the light intensity to be 150 μmol·m⁻¹. -2 ·s -1 50 μmol·m -2 ·s -1 and 50 μmol·m -2 ·s -1 The total light intensity is 250 μmol·m -2 ·s -1 The photoperiod is 12h / d; the red light wavelength is 640nm, the blue light wavelength is 440nm, and the far-red light wavelength is 730nm; the humidity of the seedling substrate is 22%, and the ambient temperature for seedling cultivation is 25℃ during the day and 15℃ at night.

[0035] Comparative Example 1

[0036] The similarities between this comparative example and the embodiment will not be repeated here. The difference is that the soil respiration rate of the substrate prepared in this comparative example is 0.5 μmol·m⁻¹. -2 ·s -1 .

[0037] Comparative Example 2

[0038] The similarities between this comparative example and the embodiment will not be repeated here. The difference is that in this comparative example, the light environment for *Prunus triloba* during the seedling stage, leaf area index ≥4, and leaf area index ≥10 growth stages is as follows: the light quality ratio of red light, blue light, and far-red light is 5:1:1, and the light intensity is 115 μmol·m⁻¹. -2 ·s -1 23 μmol·m -2 ·s -1 and 23 μmol·m -2 ·s -1 The total illuminance was 161 μmol·m. -2 ·s -1 The photoperiod is 10h / d; the red light wavelength is 640nm, the blue light wavelength is 440nm, and the far-red light wavelength is 730nm.

[0039] Comparative Example 3

[0040] This comparative example shows the growth of Prunus triloba in its natural environment.

[0041] In conjunction with the examples and comparative examples, seedling substrate and light tests were conducted on *Prunus triloba* seedlings. The morphological characteristics and chlorophyll content of *Prunus triloba* seedlings from Examples 1, 2, and 3 after 30 days of cultivation were evaluated. The experimental results are shown in Table 1.

[0042] Table 1

[0043]

[0044]

[0045] Morphology and chlorophyll content of Prunus triloba were monitored 30 days after sowing. The results showed that, compared with Comparative Examples 1, 2 and 3, the Prunus triloba with better morphological characteristics and healthier plants could be cultivated through this example.

[0046] Based on the requirement that *Prunus triloba* seedlings must be >35cm tall, the time required for the germination rate to reach this requirement was recorded for both the example and comparative studies. The experimental results are shown in Table 2.

[0047] Table 2

[0048] deal with Example Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Seedling time / day 121 134 156 147 247

[0049] The experimental results from sowing to emergence of Prunus triloba showed that, compared with Comparative Examples 1, 2 and 3, the implementation example can shorten the seedling cycle of Prunus triloba and avoid the impact of outdoor overwintering.

[0050] While the specific embodiments of the present invention have been described in detail above, the scope of protection of the present invention is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in the present invention should be included within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the scope of the claims.

Claims

1. A method for closed-loop, factory-style seedling cultivation of Prunus triloba, characterized in that, Includes the following steps: (1) Seed germination treatment: 7 to 15 days before sowing, soak the selected seeds in a 250 mg / L gibberellin solution for 2 to 3 days to promote germination; (2) Determination of CO2 saturation point of Prunus triloba: CO2 gradient experiments were conducted at various growth stages of Prunus triloba under suitable temperature, humidity and light conditions to determine the CO2 saturation point at each stage. During the seedling stage of Prunus triloba, the optimal air temperature, soil moisture and light intensity were 20℃, 18% and 100 μmol·m, respectively. -2 ·s -1 The CO2 saturation point of *Prunus triloba* was measured to be 106 μmol·mol⁻¹. -1 When the leaf area index of *Prunus triloba* is ≥4, the optimal air temperature, soil moisture, and light intensity are 20℃, 20%, and 161 μmol·m⁻², respectively. -2 ·s -1 The CO2 saturation point of *Prunus triloba* was measured to be 643 μmol·mol⁻¹. -1 When the leaf area index of *Prunus triloba* is ≥10, the optimal air temperature, soil moisture, and light intensity are 20℃, 22%, and 250 μmol·m⁻², respectively. -2 ·s -1 The CO2 saturation point of *Prunus triloba* was measured to be 1607 μmol·mol⁻¹. -1 ; (3) Measurement of the respiration rate of the seedling substrate and *Prunus triloba*: The soil respiration rate of the seedling substrate and the respiration rate of *Prunus triloba* at various growth stages under suitable temperature, humidity and light conditions were measured. The ratio of the seedling substrate was adjusted to achieve a soil respiration rate of 1.5 μmol·m⁻¹. -2 ·s -1 During the seedling stage of *Prunus triloba*, the optimal air temperature, soil moisture, and light intensity are 25℃, 18%, and 100 μmol·m⁻², respectively. -2 ·s -1 The CO2 saturation point of *Prunus triloba* was measured to be 106 μmol·mol⁻¹. -1 The respiration rates of the seedling substrate and the elm-leaf plum were measured to be 1.2 and 0.3 μmol·m⁻¹, respectively. -2 ·s -1 When the leaf area index of *Prunus triloba* is ≥4, the optimal air temperature, soil moisture, and light intensity are 25℃, 20%, and 161 μmol·m⁻², respectively. -2 ·s -1 The respiration rates of the seedling substrate and the elm-leaf plum were measured to be 1.1 and 0.5 μmol·m⁻¹, respectively. -2 ·s -1 When the leaf area index of *Prunus triloba* is ≥10, the optimal air temperature, soil moisture, and light intensity are 25℃, 22%, and 250 μmol·m⁻¹, respectively. -2 ·s -1 The respiration rates of the seedling substrate and the elm-leaf plum were measured to be 0.9 and 0.8 μmol·m⁻¹, respectively. -2 ·s -1 ; (4) Seedling substrate: The seedling substrate for Prunus triloba is made from organic waste materials that have been sterilized at high temperature, combined with clay soil, water-retaining agent and stabilizer. The ratio of the seedling substrate is adjusted so that the soil respiration rate from sowing to emergence is 1.0~3.0 μmol·m -2 ·s -1 The total amount of CO2 released daily from the seedling substrate and the respiration of the Chinese plum should match the CO2 absorption of the Chinese plum through photosynthesis. As the leaf area index of the Chinese plum increases, the photosynthetic rate also increases. By increasing the application of nitrogen fertilizer to adjust the carbon-nitrogen ratio of the seedling substrate and adding microbial agents, the soil respiration capacity can be increased accordingly, thereby improving the CO2 release capacity in the closed plant factory environment to match the CO2 required for plant photosynthesis. (5) Sowing and seedling raising: When 50% of the seeds of the elm-leaf plum show white sprouts, sow 2 seeds per hole in a fully moist substrate, with the sowing depth being 2-3 cm from the surface of the substrate; (6) Seedling emergence period: After the seedlings of Prunus triloba have emerged from the soil, turn on the plant lights to illuminate the Prunus triloba. The light quality ratio of red light, blue light and far-red light is 8:1:1, and the light intensity is 80 μmol·m⁻¹. -2 ·s -1 10 μmol·m -2 ·s -1 and 10 μmol·m -2 ·s -1 The total light intensity is 100 μmol·m -2 ·s -1 The photoperiod is 8 h / d; (7) Leaf area index of Prunus triloba ≥4: When the leaf area index of Prunus triloba ≥4, adjust the light quality ratio of red light, blue light and far-red light of the plant lamp to 5:1:1, and the light intensity to 115 μmol·m -2 ·s -1 23 μmol·m -2 ·s -1 and 23 μmol·m -2 ·s -1 The total illuminance was 161 μmol·m -2 ·s -1 The photoperiod is 10 h / d; (8) Leaf area index of Prunus triloba ≥10: When the leaf area index of Prunus triloba ≥10, adjust the light quality ratio of red light, blue light and far-red light of the plant lamp to 3:1:1, and the light intensity to 150 μmol·m -2 ·s -1 50 μmol·m -2 ·s -1 and 50 μmol·m -2 ·s -1 The total light intensity was 250 μmol·m -2 ·s -1 The photoperiod is 12 h / d.

2. The method for closed-system factory-style seedling cultivation of Prunus triloba according to claim 1, characterized in that, In step (4), the volume ratio of organic waste material, clay soil, water-retaining agent and stabilizer in the elm-leaf plum seedling substrate is 40~50%: 50~60%: 0.2~0.5%: 0.05~0.1%.

3. The method for closed-system factory-style seedling cultivation of Prunus triloba according to claim 1, characterized in that, In steps (6), (7) and (8), the red light wavelength is 640 nm, the blue light wavelength is 440 nm, and the far-red light wavelength is 730 nm.

4. The method for closed-loop factory-style seedling cultivation of Prunus triloba according to claim 1, characterized in that, The ambient temperature for seedling cultivation is 25℃ during the day and 15℃ at night.

5. The method for closed-system factory-style seedling cultivation of Prunus triloba according to claim 1, characterized in that, In step (5), the moisture content of the seedling substrate is 40-45%.

6. The method for closed-system factory-style seedling cultivation of Prunus triloba according to claim 1, characterized in that, In steps (6), (7) and (8), the moisture content of the seedling substrate is 18-25%.

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