A micro-zooplankton, a preparation method and a method for targeting radical treatment of cyanobacterial bloom

Abstract

The application discloses a kind of microphage, preparation method and its method for targeting radical cure cyanobacterial bloom, belong to surface water pollution repair / governance field.The preparation method of microphage is as follows: after phage, Fe (III) flocculant and biological surfactant are mixed, the pH value of system is adjusted to neutral, then high-speed stirring is obtained to the surface of microphage by Fe (III) and its hydrolyzed form adhering phage.After pumping microphage into the surface of surface water to be treated, after producing algal residue, by scraping residue, collection removes the algal residue that floats to the surface of water, and the targeting radical cure of cyanobacterial bloom is completed.The method can efficiently enrich, target kill, quickly separate blue-green algae and its residue, successfully eradicate the possibility of water bloom outbreak again;Using phage and biological surfactant, the amount of chemical flocculant is reduced, secondary pollution of water body is avoided, and a reliable method for removing water bloom cyanobacteria is provided.

Description

Technical Field

[0001] This invention belongs to the field of surface water pollution remediation / treatment, specifically involving a micro algae phagocytizer, its preparation method, and a method for targeted eradication of cyanobacterial blooms. Background Technology

[0002] The massive proliferation of cyanobacteria (blue-green algae) in surface water has triggered widespread freshwater blooms, severely impacting aquatic ecosystems and threatening human health and water safety. Although countries worldwide, including my country, are actively addressing the problem, cyanobacterial blooms remain severe and fluctuate at high levels. Taking Taihu Lake as an example, cyanobacterial blooms occur for 11-12 months each year, with the largest bloom area exceeding 1403 km². 2 The algae density exceeds 9.2 × 10⁻⁶. 7 per L.

[0003] Common methods for treating algal blooms are mainly divided into chemical, biological, and physical methods. Chemical methods require adding large amounts of chemical reagents to the water. While this treats algae to some extent, it also introduces a large amount of chemicals into the aquatic environment, easily causing secondary pollution. Physical methods for treating algal blooms use filtration and harvesting, which can directly and effectively remove algae from the water. However, these methods are time-consuming, costly, and only affect a localized area, failing to address the root cause of the problem.

[0004] Algophages are a type of bacteriophage that specifically infects and lyses cyanobacteria, acting as natural enemies of cyanobacteria. They participate in regulating the seasonal fluctuations of cyanobacteria and intervene in the outbreak of cyanobacterial blooms, thus showing promise as an environmentally friendly tool for controlling blooms. However, conventional methods of using algophages to control cyanobacteria require a long timeframe, failing to quickly and efficiently resolve bloom problems, and also impacting the aquatic ecosystem. Therefore, there is an urgent need to provide a method for effectively and thoroughly controlling cyanobacterial blooms in surface water using algophages. Summary of the Invention

[0005] The purpose of this invention is to overcome the shortcomings of existing technologies and provide a method for targeted and radical control of cyanobacterial blooms in surface water using a micro-algae phagocytizer. The advantage of this method over conventional treatment techniques lies in its ingenious integration of physicochemical treatment, microbial remediation, and physical separation into a single process, making it environmentally friendly and requiring minimal reagent dosage. This method enables rapid, efficient, and targeted removal of cyanobacteria.

[0006] The specific technical solution adopted in this invention is as follows:

[0007] In a first aspect, the present invention provides a method for preparing a miniature algae phagocytizer, as detailed below:

[0008] After mixing algae phages, Fe(III) flocculants, and biosurfactants, the pH of the system was adjusted to neutral to obtain a microalgae phagocyte preparation solution. The concentrations of algae phages, Fe(III) flocculants, and biosurfactants in the microalgae phagocyte preparation solution were 1.3 × 10⁻⁶. 7 ~2×10 9 PFU / L, 1–16 mmol / L (calculated as Fe), 16.7–700 mg / L; then the microalgae phagocyte preparation solution was stirred at a high speed of 10,000–30,000 rpm to obtain a microalgae phagocyte suspension with Fe(III) and its hydrolyzed form adhering to the surface.

[0009] Preferably, the algophage is one of the following:

[0010] A highly efficient lytic phycophage of Nostoc, named YongM, was deposited on January 13, 2020, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 18383. Information on this phycophage has been published in Chinese Invention Patent No. CN 113736745B, filed on December 3, 2021.

[0011] A broad-spectrum, virulent phycophage, named Me-ZS1, was deposited on November 28, 2018, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 16812. Information about this phycophage has been published in Chinese invention patent application CN 111269891A, filed on June 12, 2020.

[0012] A lytic phycophage, named MACPNOA1, was deposited on October 24, 2016, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 12996. Information on this phycophage has been published in Chinese Invention Patent No. CN 109097339B, filed on December 28, 2018.

[0013] The single-strand DNA cyanophage strain MaSSC-P from *Microcystic aeruginosa* was deposited on May 25, 2015, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 10598. Information on this cyanophage has been published in Chinese Invention Patent No. CN 105176933B, filed on December 23, 2015.

[0014] A broad-spectrum virulent cyanophage (Microcystisaeruginosa cyanophage), named MinS1, was deposited on September 7, 2021, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 23089. This has been disclosed in Chinese Invention Patent No. CN 113862228B, with a publication date of December 31, 2021.

[0015] Preferably, the Fe(III) flocculant is one of ferric chloride, ferric sulfate, polyferric chloride, polyferric sulfate, polyferric silicate, polyferric aluminum chloride, polyferric aluminum sulfate, or polyferric aluminum silicate.

[0016] Preferably, the biosurfactant is anionic or nonionic rhamnolipid, sophorolipid, or saponin.

[0017] Preferably, the high-speed stirring time is 0.5 to 2 minutes.

[0018] Secondly, the present invention provides a microalgae phagocytother prepared using the preparation method described in the first aspect.

[0019] Thirdly, the present invention provides a method for targeted eradication of cyanobacterial blooms using the microalgae phagocytes described in the second aspect, specifically as follows: a microalgae phagocyte suspension is pumped into the surface water of the area to be treated where cyanobacterial blooms are present, below the water surface, so that the microalgae phagocyte suspension rapidly diffuses and enriches cyanobacterial cells, and the algae phagocytes target and infect and kill the cyanobacterial cells, producing algal residue; after the microalgae phagocyte suspension is completely introduced into the water body, it reacts for 5-10 minutes, and then the algal residue floating to the water surface is removed by scraping and collecting; the volume ratio of the microalgae phagocyte suspension to the surface water to be treated is (0.5-3):10.

[0020] Preferably, the concentrations of algae phages, Fe(III) flocculants, and biosurfactants are determined based on the density of cyanobacteria in the surface water to be treated; when the density of cyanobacteria in the surface water to be treated is less than 4 × 10⁻⁶, the concentrations are determined based on the density of cyanobacteria. 6 When the concentration of algae phagosomes is 4 × 10⁶ mg / L, ensure that after pumping in the micro algae phagosomes, the concentration of biosurfactants in the surface water to be treated is 5–20 mg / L, and the concentration of algae phagosomes is 4 × 10⁶ mg / L. 6 ~4×10 7 The concentration of PFU / L and Fe(III) flocculant is 0.3–0.5 mmol / L, calculated as Fe;

[0021] When the density of cyanobacteria in the surface water to be treated is in the range of 4×10 6 ~1×10 7 When the concentration of algae is 1 × 10⁶ mg / L, ensure that after pumping in the micro algaecide, the concentration of biosurfactant in the surface water to be treated is 20–35 mg / L, and the concentration of algae is 1 × 10⁶ mg / L.7 ~1×10 8 The concentration of PFU / L and Fe(III) flocculant is 0.5–0.8 mmol / L, calculated as Fe.

[0022] Preferably, the rate at which the micro algaecide is pumped into the surface water to be treated is 1 L / min to 4 L / min.

[0023] As a preferred method, the micro algaecide is pumped into the surface water to be treated from 0.3m to 0.8m below the water surface.

[0024] Compared with the prior art, the present invention has the following advantages:

[0025] (1) This invention provides a method for preparing a micro algaecide. This method utilizes Fe(III) flocculant to adhere cyanobacteria phagocytes (nanoscale particles) to the surface of microbubbles, cleverly integrating the three processes of microbubble capture of cyanobacteria, phagocyte killing of cyanobacteria, and microbubble floatation to separate pollutants into a single treatment process. Applying this micro algaecide to the treatment of cyanobacterial blooms in surface water can significantly improve treatment efficiency and effectiveness.

[0026] (2) Because the surface of the micro algaecide is loaded with Fe flocculant and algae, the simultaneous implementation of biological and physicochemical methods improves the utilization efficiency of both flocculant and algae, thereby reducing the amount of both used. This not only reduces the cost of algae removal but also reduces secondary pollution from chemicals.

[0027] (3) The method for targeted eradication of cyanobacterial blooms provided by this invention directly pumps in a micro algaecide. Apart from this, the method does not require separate dosing of other drugs or microorganisms (algaecides), and the problem of re-emergence of cyanobacterial blooms in the treated water area can be completely eliminated. In a specific embodiment, algal density monitoring was conducted in the treated bloom area for 15–20 days, and no risk of re-emergence was found.

[0028] (4) The method for targeted eradication of cyanobacterial blooms provided by the present invention has a good treatment effect on surface water cyanobacterial blooms with different cyanobacterial densities. Therefore, the present invention can be used to treat them during the early warning period, the initial stage and the outbreak period of the bloom. Detailed Implementation

[0029] The present invention will be further described and illustrated below with reference to specific embodiments. The technical features of each embodiment of the present invention can be combined accordingly, provided that there is no mutual conflict.

[0030] Example 1

[0031] This embodiment provides a method for targeted and radical control of cyanobacterial blooms in surface water using micro-algae phagocytics, as detailed below:

[0032] In this embodiment, the density of cyanobacteria in the surface water to be treated is 5 × 10⁻⁶. 6 / L. In this embodiment, bacteriophage No. 1, a broad-spectrum virulent algaeophage, was used and named Me-ZS1. It was deposited at the China General Microbiological Culture Collection Center (CGMCC) on November 28, 2018, with accession number CGMCC No. 16812. Information on this algaeophage has been published in Chinese invention patent application CN 111269891A, with a publication date of June 12, 2020.

[0033] (1) Preparation of microalgae phagocytic microbubble system

[0034] After mixing algae 1, FeCl3 and nonionic rhamnose in a certain proportion, the pH of the system was adjusted to neutral to obtain the microalgae phagocyte preparation solution. Then, the microalgae phagocyte preparation solution was stirred at high speed at a speed of 12000 rpm for 1 min to obtain microalgae phagocytes with Fe(III) and its hydrolyzed form adhering to the surface.

[0035] (2) The prepared microalgae phagocytizer was pumped into the surface water to be treated at a depth of 0.70 m below the water surface at a flow rate of 3.5 L / min. The density of cyanobacteria in the surface water to be treated was 5 × 10⁻⁶. 6 Therefore, ensure that the concentration of nonionic rhamnolipids is 25 mg / L, the concentration of FeCl3 is 0.6 mmol / L, and the concentration of bacteriophages is 5 × 10⁻⁶ mg / L when pumped into the surface water to be treated. 7 PFU / L.

[0036] The microalgae phagocytes rapidly diffused and enriched cyanobacterial cells in the surface water to be treated, and the phagocytes targeted and killed the cyanobacterial cells. After 5 minutes, water samples were taken at a depth of 0.70m to measure relevant indicators, and the results are shown in Table 1. At the same time, a sludge scraper was used to scrape and collect the algal residue on the water surface and remove it from the water body.

[0037] Comparative Example 1

[0038] This comparative example provides a method for controlling cyanobacterial blooms in surface water, as follows:

[0039] (1) Preparation of microbubble suspension

[0040] Mix the algae 1 and FeCl3 in a moderate dose as in Example 1 and adjust the pH of the system to neutral. After large aggregates are formed, add the nonionic rhamnose lipolipase in a moderate dose as in Example 1. Then stir at high speed for 10,000 to 30,000 rpm for 1 min to obtain a microbubble suspension.

[0041] (2) The prepared microbubble suspension was pumped into the surface water to be treated at a depth of 0.70 m below the water surface at a flow rate of 3.5 L / min. The density of cyanobacteria in the surface water to be treated was 5 × 10⁻⁶. 6 Therefore, ensure that the concentration of nonionic rhamnolipids is 25 mg / L, the concentration of FeCl3 is 0.6 mmol / L, and the concentration of bacteriophages is 5 × 10⁻⁶ mg / L when pumped into the surface water to be treated. 7 PFU / L. After 5 minutes, a water sample was taken at a depth of 0.70m to measure relevant indicators. The results are shown in Table 1. At the same time, a sludge scraper was used to scrape and collect algae residue from the water surface and remove it from the water body.

[0042] In this comparative example, the density of cyanobacteria in the surface water to be treated and the amount of bacteriophage used were the same as in Example 1, as were the amounts of FeCl3, algae phage No. 1, and nonionic rhamnolipid.

[0043] Table 1 Comparison of treatment effects between Example 1 and Comparative Example 1

[0044]

[0045] As shown in Table 1, under the same dosage of flocculant, biosurfactant and algae phage, according to the results of Comparative Example 1, the flocculant and algae phage in the water first aggregate with the cyanobacterial cells and are then separated by microbubbles. Their algae removal and algae regeneration inhibition effects are significantly lower than the treatment effect of Example 1 of the present invention.

[0046] Example 2

[0047] This embodiment provides a method for targeted and radical control of cyanobacterial blooms in surface water using micro-algae phagocytics, as detailed below:

[0048] In this embodiment, the density of cyanobacteria in the surface water to be treated is 3 × 10⁻⁶. 6 / L. The bacteriophage No. 2 used in this embodiment is a lytic algal phage named MACPNOA1, which was deposited at the China General Microbiological Culture Collection Center on October 24, 2016, with accession number CGMCC No. 12996. Information on this algal phage has been disclosed in Chinese invention patent application published on December 28, 2018, with authorization announcement number CN 109097339B.

[0049] (1) Preparation of microalgae phagocytic microbubble system

[0050] After mixing algae 2, polyferric chloride (PFCl), and nonionic sophorolipid in a certain proportion, the pH of the system was adjusted to neutral to obtain a microalgae phagocyte preparation solution. Then, the microalgae phagocyte preparation solution was stirred at high speed at a speed of 25,000 rpm for 1.5 min to obtain a microalgae phagocyte with the surface of which the bacteriophage is adhered by Fe(III) and its hydrolyzed form.

[0051] (2) The prepared microalgae phagocytogen was pumped into the surface water to be treated at a depth of 0.50 m below the water surface at a flow rate of 1 L / min. The density of cyanobacteria in the surface water to be treated was 5 × 10⁻⁶. 6 Therefore, ensure that the concentration of nonionic sophorolipids is 20 mg / L, the concentration of polyferric chloride (PFCl) is 0.4 mmol / L, and the concentration of bacteriophage No. 2 is 2 × 10⁻⁶ mg / L when pumping into the surface water to be treated. 7 PFU / L.

[0052] The microalgae phagocytes rapidly diffused and enriched cyanobacterial cells in the surface water to be treated, and the phagocytes targeted and killed the cyanobacterial cells. After 8 minutes, water samples were taken at a depth of 0.50m to measure relevant indicators, and the results are shown in Table 2. At the same time, a sludge scraper was used to scrape and collect the algal residue on the water surface and remove it from the water body.

[0053] Comparative Example 2

[0054] This comparative example provides a method for controlling cyanobacterial blooms in surface water, as follows:

[0055] (1) Preparation of microbubble suspension

[0056] Mix the algae 2 phage and polyferric chloride (PFCl) in a moderate dose as in Example 2 and adjust the pH of the system to neutral. After large aggregates are formed, add the nonionic sophorolipid in a moderate dose as in Example 2. Then stir at high speed for 1.5 min at a stirring rate of 25,000 rpm to obtain a microbubble suspension.

[0057] (2) The prepared microbubble suspension was pumped into the surface water to be treated at a depth of 0.50 m below the water surface at a flow rate of 1 L / min. The density of cyanobacteria in the surface water to be treated was 3 × 10⁻⁶. 6 The concentration of nonionic sophorolipids was ensured to be 20 mg / L, polyferric chloride (PFCl) to be 0.4 mmol / L, and phage No. 2 to be 2 × 10⁷ PFU / L when pumped into the surface water to be treated. Water samples were taken at a depth of 0.50 m after 8 minutes to measure relevant indicators; the results are shown in Table 2. Simultaneously, a sludge scraper was used to collect algal debris from the water surface and remove it from the water body.

[0058] In this comparative example, the density of cyanobacteria in the surface water to be treated and the amount of bacteriophage No. 2 used are the same as in Example 2. The amounts of polyferric chloride (PFCl), bacteriophage No. 2, and nonionic sophorolipid are also the same as in Example 2.

[0059] Table 2 Comparison of treatment effects between Example 2 and Comparative Example 2

[0060]

[0061] As shown in Table 2, under the same dosage of flocculant, biosurfactant and algae phage, according to the results of Comparative Example 1, the flocculant and algae phage in the water first aggregate with the cyanobacterial cells and are then separated by microbubbles. Their algae removal and algae regeneration inhibition effects are significantly lower than the treatment effect of Example 2 of the present invention.

[0062] Example 3

[0063] This embodiment provides a method for targeted and radical control of cyanobacterial blooms in surface water using micro-algae phagocytics, as detailed below:

[0064] In this embodiment, the density of cyanobacteria in the surface water to be treated is 7 × 10⁻⁶. 5 / L. In this embodiment, bacteriophage No. 3, a broad-spectrum virulent algae phage (Microcystis aeruginosa cyanophage), named MinS1, was deposited at the China General Microbiological Culture Collection Center on September 7, 2021, with accession number CGMCC No. 23089. This has been disclosed in Chinese invention patent application published on December 31, 2021, with authorization announcement number CN 113862228B.

[0065] (1) Preparation of microalgae phagocytic microbubble system

[0066] After mixing algae 3, polyferric silicate (PFSi) and biosurfactant saponin in a certain proportion, the pH of the system was adjusted to neutral to obtain the micro algae phagocyte preparation solution. Then, the micro algae phagocyte preparation solution was stirred at high speed at a speed of 30,000 rpm for 2 min to obtain micro algae phagocytes with Fe(III) and its hydrolyzed form adhering to the surface.

[0067] (2) The prepared microalgae phagocytogen was pumped into the surface water to be treated at a depth of 0.60 m below the water surface at a flow rate of 1.2 L / min. The density of cyanobacteria in the surface water to be treated was 5 × 10⁻⁶. 6 Therefore, ensure that the concentration of saponins pumped into the surface water to be treated is 10 mg / L, the concentration of polyferric silicate (PFSi) is 0.35 mmol / L, and the concentration of bacteriophage No. 3 is 1 × 10⁻⁶. 6 PFU / L.

[0068] The microalgae phagocytes rapidly diffused and enriched cyanobacterial cells in the surface water to be treated, and the phagocytes targeted and killed the cyanobacterial cells. After 10 minutes, water samples were taken at a depth of 0.60m to measure relevant indicators, and the results are shown in Table 3. At the same time, a sludge scraper was used to scrape and collect the algal residue on the water surface and remove it from the water body.

[0069] Comparative Example 3

[0070] This comparative example provides a method for controlling cyanobacterial blooms in surface water, as follows:

[0071] (1) Preparation of microbubble suspension

[0072] Mix the algae 3 and polyferric silicate (PFSi) in a moderate dose as in Example 3 and adjust the pH of the system to neutral. After large aggregates are formed, add the saponin in a moderate dose as in Example 2. Then stir at high speed for 2 minutes at a stirring rate of 30,000 rpm to obtain a microbubble suspension.

[0073] (2) The prepared microbubble suspension was pumped into the surface water to be treated at a depth of 0.60 m below the water surface at a flow rate of 1.2 L / min. The density of cyanobacteria in the surface water to be treated was 5 × 10⁻⁶. 6 Therefore, ensure that the concentration of saponins pumped into the surface water to be treated is 10 mg / L, the concentration of polyferric silicate (PFSi) is 0.35 mmol / L, and the concentration of bacteriophage No. 3 is 1 × 10⁻⁶. 6 PFU / L. After 10 minutes, a water sample was taken at a depth of 0.60m to measure relevant indicators. The results are shown in Table 3. At the same time, a sludge scraper was used to scrape and collect algae residue from the water surface and remove it from the water body.

[0074] In this comparative example, the density of cyanobacteria in the surface water to be treated and the use of bacteriophage No. 3 are the same as in Example 3. The amounts of polyferric silicate (PFSi), bacteriophage No. 3, and saponins are also the same as in Example 3.

[0075] Table 3 Comparison of treatment effects between Example 3 and Comparative Example 3

[0076]

[0077] As shown in Table 3, under the same dosage of flocculant, biosurfactant and algae phage, according to the results of Comparative Example 3, the flocculant and algae phage in the water first aggregate with the cyanobacterial cells and are then separated by microbubbles. Their algae removal and algae regeneration inhibition effects are significantly lower than the treatment effect of Example 3 of the present invention.

[0078] In summary, the method provided by this invention can target, kill, and efficiently separate harmful cyanobacteria in surface water, thus completely controlling cyanobacterial blooms. First, cyanobacterial phages are combined with flocculants and biosurfactants to form a complex system and prepared into microbubbles. The phages adhere to the surface of the microbubbles through the flocculant, thereby forming micro-algebras. Subsequently, after introducing the micro-algebras into surface water areas during the early warning or outbreak period of cyanobacterial blooms, the cyanobacteria are rapidly captured on the surface of the microbubbles and inactivated by the specific infection of the phages on the surface. Simultaneously, the cyanobacterial cells float to the water surface with the microbubbles and are removed through sediment-liquid separation.

[0079] This invention utilizes a microalgae phagocytes, composed of algae phagocytes, flocculants, and biosurfactants in a specific ratio. These microalgae phagocytes carry a positively charged surface, enabling rapid and efficient (approximately 5-10 minutes) targeted killing and separation of cyanobacteria in algal blooms (removal rate >3.01g). Furthermore, algal density measurements of the treated area within 15-20 days revealed no algal regeneration, indicating that this method also inhibits the recurrence of algal blooms. The technology involved in this invention efficiently enriches, targets, and rapidly separates cyanobacteria and their residues, successfully eradicating the possibility of recurring algal blooms. The use of algae phagocytes and biosurfactants reduces the amount of chemical flocculants used in the system, avoiding secondary water pollution and providing a reliable method for removing cyanobacteria blooms.

[0080] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the invention. Those skilled in the art can make various changes and modifications without departing from the spirit and scope of the invention. Therefore, all technical solutions obtained through equivalent substitution or transformation fall within the protection scope of the present invention.

Claims

1. A method for preparing a microalgae phagocytotherm, characterized in that, Specifically as follows: After mixing algae phages, Fe(III) flocculants, and biosurfactants, the pH of the system was adjusted to neutral to obtain a microalgae phagocyte preparation solution. The concentrations of algae phages, biosurfactants, and Fe(III) flocculants in the microalgae phagocyte preparation solution were 1.3 × 10⁻⁶. 7 ~2×10 9 PFU / L, 16.7~700 mg / L and 1~16 mmol / L, wherein the concentration of Fe(III) flocculant is expressed as Fe; then the microalgae phagocyte preparation solution is stirred at high speed at a stirring rate of 10000~30000 rpm to obtain a microalgae phagocyte suspension with Fe(III) and its hydrolyzed form adhering to the surface of the bacteriophage; Fe(III) flocculant is used to adhere nanoscale algae phages of cyanobacteria to the surface of micron-sized bubbles, integrating the three processes of microbubble capture of cyanobacteria, algae phage killing of cyanobacteria, and microbubble floatation to separate pollutants into a single treatment process.

2. The method for preparing the microalgae phagocytotherm according to claim 1, characterized in that, The algophage is one of the following: A highly efficient lytic phycophage of *Nostoc*, named YongM, was deposited at the China General Microbiological Culture Collection Center (CGMCC) on January 13, 2020, with accession number CGMCC No. 18383; a broad-spectrum virulent phycophage, named Me-ZS1, was deposited at the CGMCC on November 28, 2018, with accession number CGMCC No. 16812; a lytic phycophage, named MACPNOA1, was deposited at the CGMCC on October 24, 2016, with accession number CGMCC No. 12996; and a single-stranded DNA phycophage strain of *Microcystis aeruginosa*, MaSSC-P, was deposited at the CGMCC on May 25, 2015, with accession number CGMCC No. 10598; A broad-spectrum, highly virulent phycophage, named MinS1, was deposited on September 7, 2021, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 23089.

3. The method for preparing the microalgae phagocytotherm according to claim 1, characterized in that, The Fe(III) flocculant is one of ferric chloride, ferric sulfate, polyferric chloride, polyferric sulfate, or polyferric silicate.

4. The method for preparing the microalgae phagocytotherm according to claim 1, characterized in that, The biosurfactant is anionic or nonionic rhamnolipid, sophorolipid, or saponin.

5. The method for preparing the microalgae phagocytizer according to claim 1, characterized in that, The high-speed stirring time is 0.5~2 min.

6. A microalgae phagocytizer prepared using any one of the preparation methods described in claims 1 to 5.

7. A method for targeted eradication of cyanobacterial blooms using the microalgae phagocytizer described in claim 6, characterized in that, Specifically, the following steps are taken: a suspension of microalgae phagocytosors is pumped into the surface water below the water surface where cyanobacterial blooms are present. This allows the microalgae phagocytosors suspension to rapidly diffuse and enrich cyanobacterial cells. The algae phagocytoses then target and kill the cyanobacterial cells, producing algal residue. After the microalgae phagocytosors suspension is completely introduced into the water body, the reaction takes 5-10 minutes. Subsequently, the algal residue that floats to the water surface is removed by scraping and collecting. The volume ratio of the microalgae phagocytosors suspension to the surface water is (0.5-3):

10.

8. The method for targeted eradication of cyanobacterial blooms according to claim 7, characterized in that, The concentrations of algae phages, Fe(III) flocculants, and biosurfactants are determined based on the density of cyanobacteria in the surface water to be treated; when the density of cyanobacteria in the surface water to be treated is less than 4 × 10⁻⁶, the concentrations are determined based on the density of cyanobacteria. 6 When the concentration of algae phagosomes is 4 × 10⁶ mg / L, ensure that after pumping in the micro algae phagosomes, the concentration of biosurfactants in the surface water to be treated is 5-20 mg / L and the concentration of algae phagosomes is 4 × 10⁶ mg / L. 6 ~4×10 7 The concentration of PFU / L and Fe(III) flocculant is 0.3~0.5 mmol / L, calculated as Fe; When the density of cyanobacteria in the surface water to be treated is in the range of 4×10 6 ~1×10 7 When the concentration of algae is 1 × 10⁶ mg / L, ensure that after pumping in the micro algaecide, the concentration of biosurfactant in the surface water to be treated is 20-35 mg / L, and the concentration of algae is 1 × 10⁶ mg / L. 7 ~1×10 8 The concentration of PFU / L and Fe(III) flocculant is 0.5~0.8 mmol / L, calculated as Fe.

9. The method for targeted eradication of cyanobacterial blooms according to claim 7, characterized in that, The rate at which the microalgae phagocytizer is pumped into the surface water to be treated is 1 L / min-4 L / min.

10. The method for targeted eradication of cyanobacterial blooms according to claim 7, characterized in that, The micro algaecide is pumped into the surface water to be treated from 0.3 m to 0.8 m below the water surface.

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