An apparatus for rearing larval parasitoid wasps

Abstract

This invention discloses an apparatus and method for propagating parasitic wasps larvae. The apparatus includes a non-transparent bottom box, a transparent cover, and a non-transparent cover, arranged sequentially from bottom to top and capable of being sealed together. The non-transparent bottom box has an opening, and a first inlet on its side wall connects to its internal chamber. A second inlet corresponding to the internal chamber is provided on the transparent cover, such that when the transparent cover is placed over the opening of the non-transparent bottom box, the second inlet connects to the internal chamber. A third inlet corresponding to the second inlet is provided on the non-transparent cover. The apparatus also includes a first plug capable of sealing the first inlet and a first transparent adapter detachably connected to the first inlet. Furthermore, it includes a second plug capable of sealing the second inlet and a second transparent adapter that passes through the third inlet and is detachably connected to the second inlet. This apparatus and method offer significant advantages in terms of labor time, parasitism rate, and cocoon harvesting rate, significantly reducing propagation costs and improving propagation efficiency.

Description

Technical Field

[0001] This invention relates to the field of parasitic wasp propagation devices, and more particularly to a device for propagating parasitic wasp larvae. Background Technology

[0002] Lepidoptera pests belonging to the family Noctuidae are widely distributed across various countries and regions. Representative species include: *Spodoptera litura Fabricius*, *Spodoptera frugiperda*, *Spodoptera exigua*, and *Helicoverpa armigera*. These pests are characterized by their voracious appetite, omnivorous diet, and high reproductive rate. The larvae feed on plants, causing holes or gaps in leaves, and in severe cases, leading to plant death.

[0003] Currently, chemical control remains the primary method for controlling noctuid moths. However, the long-term use of chemical pesticides is leading to increasingly prominent problems such as excessive pesticide residues, increased pest resistance, and disruption of the ecological balance. Controlling pests through natural enemies, as an important component of biological control, is a green and safe long-term pest control method. Pest parasitic wasps, as natural enemies of pests, are an important biological control resource. Their large-scale propagation, production, and packaging can enable them to be used as biological control products for pests, offering significant application value for green pest control.

[0004] The Manila parasitic wasp *Microplitis manilae* and the spotted wasp *Meteorus pulchricornis* can parasitize lepidopteran larvae, playing a crucial role in controlling various lepidopteran pests such as the beet armyworm, cotton bollworm, fall armyworm, cabbage looper, and gypsy moth, demonstrating high potential for biological control. Both parasitic wasps lay their eggs inside lepidopteran larvae. After being parasitized, the larvae's food intake rapidly decreases. Once mature inside the wasp, the wasp larvae emerge from the lepidopteran larvae, pupate, and eventually die. This significantly reduces the damage caused by pests to plants.

[0005] Currently, there is limited research both domestically and internationally on the large-scale propagation technology of *Condor Parasiticus manilais* and *Condor Parasiticus stigmatus*. In particular, there are no ideal technical solutions regarding parasitic containers, rapid separation methods between parasitic wasps and hosts, and the susceptibility of host colonies to disease and mortality, resulting in high propagation costs. This invention / invention primarily focuses on the innovative design of parasitic and rearing containers for both the wasps and hosts, enabling them to meet the needs of large-scale production and achieve low-cost, high-efficiency propagation of parasitic wasps. Summary of the Invention

[0006] The technical problem solved by the present invention is to provide a device for propagating parasitic wasps larvae.

[0007] The specific technical solution is as follows:

[0008] An apparatus for propagating parasitic wasps larvae includes an opaque bottom box, a transparent cover, and an opaque cover arranged sequentially from bottom to top and capable of being sealed together. The opaque bottom box is open, and a first inlet is provided on its side wall, connecting to its internal chamber. The transparent cover has a second inlet corresponding to the internal chamber, such that when the transparent cover is placed over the opening of the opaque bottom box, the second inlet connects to the internal chamber. The opaque cover has a third inlet corresponding to the second inlet. The apparatus also includes a first plug capable of sealing the first inlet and a first transparent adapter body capable of detachably connecting to the first inlet. Furthermore, it includes a second plug capable of sealing the second inlet and a second transparent adapter body capable of passing through the third inlet and detachably connecting to the second inlet.

[0009] Preferably, the first inlet is a first circular opening, the second inlet is a second circular opening, and the third inlet is a third circular opening. The first transparent adapter is a first transparent adapter tube, and the second transparent adapter is a second transparent adapter tube. The first and second transparent adapter tubes are a shared transparent adapter tube, with opposite ends being a first end and a second end, respectively. The first end is used to connect to the first circular opening, and the second end is used to connect to the second circular opening. The first circular opening is directly formed on the sidewall, and the first end is shaped to match the first circular opening and has an outer sleeve. A rubber ring is provided to seal the connection to the first circular opening. A through hole is provided on the transparent cover, and an upwardly protruding hollow ring is provided at the edge of the through hole. The through hole and the internal channel of the hollow ring form the second circular opening. Two opposing arc-shaped snap-fit ​​protrusions are provided on the outer tube wall of the second end. Two opposing arc-shaped snap-fit ​​grooves corresponding to the two arc-shaped snap-fit ​​protrusions are provided on the inner side wall of the hollow ring, so that the two arc-shaped snap-fit ​​protrusions at the second end can be inserted into the second circular opening and can be rotated and snapped into the two arc-shaped snap-fit ​​grooves. The third circular opening of the non-transparent cover can be fitted onto the outer periphery of the hollow ring.

[0010] Preferably, the lower end of the hollow ring is detachably connected to the transparent cover by an adhesive bonding method.

[0011] Preferably, the shared transparent adapter pipe is in the form of a bend.

[0012] Preferably, the first and second circular openings are the same size.

[0013] Preferably, the first plug is a first round plug and the second plug is a second round plug.

[0014] Preferably, the first round plug and the second round plug are a common round plug.

[0015] Preferably, the non-transparent bottom box, the transparent cover, and the non-transparent cover are connected sequentially by a snap-fit ​​connection.

[0016] Preferably, the dimensions of the non-transparent base box are: length 20-60cm, width 15-50cm, and height 4-10cm.

[0017] The device for propagating parasitic wasps larvae provided by this invention has the following technical effects:

[0018] When in use, the device includes the following:

[0019] ① Place a measured amount of host of appropriate age and an appropriate amount of host feed in the inner chamber of the non-transparent bottom box, put in a cotton ball soaked in honey water, put the transparent cover on the non-transparent bottom box, and plug the second round opening on the transparent cover with the second round opening plug.

[0020] ② Remove the first round plug from the first round opening on the side wall of the non-transparent bottom box, connect the first transparent adapter tube, introduce the parasitic wasp into the non-transparent bottom box (using the parasitic wasp's photorepulsive and upward attraction), and finally seal the first round opening with the first round plug.

[0021] ③ Place the opaque bottom box from step ② in a rearing room under certain conditions, and the parasitic wasps will complete the parasitism process inside the box;

[0022] ④ After the parasitic process is over, place the non-transparent cap on top of the transparent cap;

[0023] ⑤ Remove the second round plug and connect the second transparent adapter tube. Use the phototaxis and uplift of the parasitic wasp to guide it into the adapter tube, and then bring out the device.

[0024] Steps ① through ⑤ above complete one parasitism cycle. This cycle repeats throughout the entire parasitism process, achieving the goal of rapid proliferation of parasitic wasps. The specific effects are as follows:

[0025] ① It can quickly and without damage separate the host and the parasitic wasp. Compared with the traditional separation methods (manual grabbing, suction of insects), it is not only more efficient, but also does not cause any damage to the parasitic wasp.

[0026] ②The breeding method using this device has significant advantages over existing breeding methods in terms of labor time, parasitism rate, and cocoon harvesting rate, and can significantly reduce breeding costs and improve breeding efficiency.

[0027] ③ It solves the problem of susceptibility of noctuid moth larvae to diseases during large-scale propagation (virus, fungal, and bacterial infections of noctuid moth larvae), thus ensuring the stability of large-scale propagation.

[0028] ④ This solved the problem of self-mutilation of moth larvae during large-scale propagation, reduced propagation losses, and lowered the proportion of moth larvae that were secondary contaminated by pathogens due to the decay of their bodies after self-mutilation.

[0029] ⑤ This solved the problem of moth larvae feeding on parasitic wasp cocoons during large-scale propagation, reducing propagation losses.

[0030] The present invention also provides a breeding method, which uses the aforementioned device for propagating parasitic larvae to breed parasitic larvae, comprising the following:

[0031] Place a measured amount of host of appropriate age and an appropriate amount of host feed in the non-transparent bottom box, add a cotton ball soaked in honey water, place the transparent cover on the non-transparent bottom box, and plug the second round opening on the transparent cover with the second round opening plug.

[0032] Remove the first round plug from the first round opening, connect the first transparent adapter tube, introduce the parasitic wasp inside the transparent adapter tube into the non-transparent bottom box, and finally close the first round opening with the first round plug.

[0033] In an insect rearing chamber under certain conditions, parasitic wasps complete the parasitism process within the device.

[0034] After the parasitic process is complete, place the non-transparent cap on top of the transparent cap;

[0035] Remove the second round plug from the transparent cap and connect the second transparent adapter tube, then introduce the parasitic wasp into the second transparent adapter tube.

[0036] As a preferred option, the following steps are also included:

[0037] Host rearing after parasitism: Single-head rearing method is adopted. The container for single-head rearing is a 32-hole rearing tray. Each hole is filled with an appropriate amount of artificial feed for the host. The host in the non-transparent bottom box is transferred to the 32-hole feed tray, one host per hole. The tray is placed on the shelf of the insect rearing room for rearing.

[0038] Harvesting bee cocoons: On the 10th-11th day after parasitism, open the breathable sealing film of the 32-hole tray, take out the bee cocoons from the holes, and collect them into the cocoon collection container. When the bee cocoons are about to emerge, transfer them to the parasitic bee rearing round box or the first transparent transfer tube, provide honey water, and wait for them to emerge for use. Attached Figure Description

[0039] Figure 1 This is a schematic diagram of the device for propagating parasitic wasps larvae provided by the present invention.

[0040] Figure 2 This is a schematic diagram of the relevant structure, including a non-transparent bottom box and a transparent cover;

[0041] Figure 3This is a schematic diagram of the provided transparent cover and hollow ring.

[0042] The following are the labels in the diagram: 1. Non-transparent base box, 2. Transparent cover, 3. Non-transparent cover, 4. First round opening, 5. Second round opening, 6. Third round opening, 7. Shared transparent adapter tube, 8. Mesh, 9. Arc-shaped snap-fit ​​protrusion, 10. Shared round opening plug, 11. Hollow ring, 12. Arc-shaped snap-fit ​​groove. Detailed Implementation

[0043] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0044] like Figure 1-3 As shown, the present invention provides an apparatus for propagating parasitic wasps larvae, comprising a non-transparent bottom box 1, a transparent cover 2, and a non-transparent cover 3 arranged sequentially from bottom to top and capable of being sealed together. The non-transparent bottom box 1 is open, and a first inlet communicating with its internal cavity is provided on the side wall of the non-transparent bottom box 1. A second inlet corresponding to the internal cavity is provided on the transparent cover 2, such that when the transparent cover 2 is placed over the opening of the non-transparent bottom box 1, the second inlet communicates with the internal cavity. A third inlet corresponding to the second inlet is provided on the non-transparent cover 3. The apparatus also includes a first plug capable of sealing the first inlet and a first transparent adapter body capable of detachably communicating with the first inlet. Furthermore, the apparatus includes a second plug capable of sealing the second inlet and a second transparent adapter body capable of passing through the third inlet and detachably communicating with the second inlet.

[0045] In one specific implementation, such as Figure 1-3 As shown, the first inlet is a first circular opening 4, the second inlet is a second circular opening 5, and the third inlet is a third circular opening 6. The first transparent adapter is a first transparent adapter tube, and the second transparent adapter is a second transparent adapter tube. The first transparent adapter tube and the second transparent adapter tube are a shared transparent adapter tube 7. The two opposite ends of the shared transparent adapter tube 7 are a first end and a second end, respectively. The first end is used to connect to the first circular opening 4, and the second end is used to connect to the second circular opening 5. The first circular opening 4 is directly formed on the side wall, and the first end is shaped to match the first circular opening 4 and is fitted with a rubber ring for... The transparent cover 2 has a through hole and an upward-protruding hollow ring 11 at the edge of the through hole. The through hole and the internal channel of the hollow ring 11 form the second circular opening 5. The outer tube wall of the second end has two opposing arc-shaped snap-fit ​​protrusions 9. The inner side wall of the hollow ring 11 has two opposing arc-shaped snap-fit ​​grooves 12 corresponding to the two arc-shaped snap-fit ​​protrusions 9, so that the two arc-shaped snap-fit ​​protrusions 9 at the second end can be inserted into the second circular opening 5 and rotated to snap into the two arc-shaped snap-fit ​​grooves 12. The third circular opening 6 of the non-transparent cover 3 can be fitted onto the outer periphery of the hollow ring 11.

[0046] When in use, the device includes the following:

[0047] ① Place a measured amount of age-appropriate host and an appropriate amount of host feed into the internal cavity of the non-transparent bottom box 1, place a cotton ball soaked in honey water, put the transparent cover 2 on top of the non-transparent bottom box 1, and plug the second round opening 5 on the transparent cover 2 with the second round opening plug.

[0048] ② Remove the first round plug at the first round opening 4 on the side wall of the non-transparent bottom box 1, connect the first transparent adapter tube, introduce the parasitic wasp into the non-transparent bottom box 1 (using the parasitic wasp's photorepulsiveness and upward attraction), and finally seal the first round opening 4 with the first round plug.

[0049] ③ Place the non-transparent bottom box 1 from step ② in a rearing room under certain conditions, and the parasitic wasps complete the parasitism process inside the box;

[0050] ④ After the parasitic process is over, place the non-transparent cover 3 on top of the transparent cover 2;

[0051] ⑤ Remove the second round plug and connect the second transparent adapter tube. Use the phototaxis and uplift of the parasitic wasp to guide it into the adapter tube, and then bring out the device.

[0052] The above steps ① to ⑤ complete one parasitism process, and this cycle repeats throughout the entire parasitism process to achieve the goal of rapid proliferation of parasitic wasps.

[0053] In one specific embodiment, the lower end of the hollow ring 11 is detachably connected to the transparent cover 2 by an adhesive bonding method.

[0054] like Figure 1 As shown, the shared transparent adapter pipe 7 is a bent pipe.

[0055] In one specific embodiment, the first circular opening 4 and the second circular opening 5 are of the same size.

[0056] The first plug is a first round plug, and the second plug is a second round plug.

[0057] In one specific embodiment, the first round plug and the second round plug are a common round plug 10.

[0058] like Figure 2 As shown, in one specific embodiment, the transparent cover 2 has a square opening in the middle, and the square opening is covered with a 60-100 mesh mesh of gauze 8.

[0059] In one specific embodiment, the non-transparent bottom box 1, the transparent cover 2, and the non-transparent cover 3 are connected sequentially by a snap-fit ​​connection.

[0060] The dimensions of the non-transparent bottom box 1 are: length 20-60cm, width 15-50cm, and height 4-10cm.

[0061] The following settings are used for propagation using this device:

[0062] (1) Rearing environment: The following rearing steps and processes are all carried out in the insect rearing room, with a photocycle of 16 hours of light and 8 hours of darkness, a temperature of 26±1℃, and a humidity of 45±5%.

[0063] (2) Host feeding: After the noctuid moth eggs hatch in the rearing box, artificial feed is provided for feeding. When they develop to the 2nd or 3rd instar, they can be used as suitable hosts for parasitic wasps to parasitize.

[0064] (3) Parasitic wasp rearing: When parasitic wasps do not need to parasitize, they are kept in parasitic wasp rearing boxes. The cotton balls soaked in 10% honey water are replaced once a day to provide nutrition for the adult parasitic wasps. When parasitic wasps need to parasitize, the cocoons that are about to emerge are placed in the "transfer tube" to wait for them to emerge. The tube is filled with cotton balls soaked in 10% honey water.

[0065] (4) Parasitism: A certain amount of host larvae of the noctuid moth are placed in the non-transparent bottom box 1, and corresponding host feed is provided. The transparent cover 2 is covered, and the parasitic wasps in the tube are introduced into the non-transparent bottom box 1 through the first round opening 4 using the first transparent adapter tube. After plugging the first round opening, the box is placed on the shelf of the insect rearing room to begin parasitism.

[0066] (5) Exiting the parasitic wasps after parasitism ends: Place the non-transparent cover 3 on top of the transparent cover 2, remove the second round plug on the transparent cover 2, connect the second transparent tube to the second round opening 5, and use phototaxis and upward attraction to introduce the parasitic wasps into the second transparent tube.

[0067] (6) Post-parasitic host rearing: Transfer the noctuid moth larvae host in the non-transparent bottom box 1 to the 36-hole paint box for single rearing, place an appropriate amount of feed in each hole, and close the lid of the 36-hole paint box.

[0068] (7) Parasitic wasp cocoon collection: On the 10th to 12th day after the host is parasitized, open the lid of the 36-hole paint box, take out the bee cocoon from the hole, and collect it into the cocoon collection container.

[0069] Example 1: Parasitism of the Manila lateral furrow wasp on the beet armyworm A

[0070] (1) The source of the test insects: the parasitic wasp was Manila lateral groove braconid wasp, and the host was the larvae of the beet armyworm.

[0071] (2) Parasite container: The dimensions of the non-transparent bottom box 1 are 35cm long, 25cm wide and 5cm high; the dimensions of the square opening of the transparent cover 2 are 25cm long and 18cm wide, and an 80-mesh mesh 8 is attached to the square opening for ventilation.

[0072] (3) Operation process:

[0073] ① Place 300 early third instar larvae of the beet armyworm in the non-transparent bottom box 1, along with an appropriate amount of blocky artificial feed for the armyworm, and two defatted cotton balls soaked in 10% honey water. Place the transparent cover 2 on top of the non-transparent bottom box 1 and plug the second round opening 5 on the transparent cover 2.

[0074] ② Transfer 30 pairs of Manila parasitic wasps that have emerged 3 days ago from the parasitic wasp rearing box to the first transparent transfer tube of the parasitic device. Use the first transparent transfer tube to introduce the parasitic wasps into the non-transparent bottom box 1. After the transfer is completed, plug the first round opening 4 to start the first day of parasitism, which lasts for 24 hours.

[0075] ③ After the parasitism ends on the first day, place the non-transparent cover 3 on top of the transparent cover 2, remove the second round plug on the second round opening 4 of the transparent cover 2 and connect the second transparent adapter tube, and introduce all 30 pairs of parasitic wasps into the second transparent adapter tube.

[0076] ④ After the parasitism ends, the larvae of the beet armyworm are transferred to a 36-cell paint box for single-head rearing in one cell. An appropriate amount of artificial feed for armyworms is placed in each cell, and then the lid of the paint box is closed.

[0077] ⑤ On the 10th to 12th day after the host is parasitized, open the lid of the 36-hole paint box, take out the bee cocoons from the holes, collect them into the cocoon collection container, and count the number of host deaths and parasitism of the noctuid moth larvae, as well as the number of cocoons collected by the parasitic wasps.

[0078] Host mortality rate = Number of host deaths / Total number of hosts

[0079] Parasite quantity: The number of noctuid moth larvae that are parasitized.

[0080] Parasitism rate = number of parasites / total number of hosts

[0081] Cocoon count: The number of parasitic wasp cocoons formed after the noctuid moth larvae are parasitized.

[0082] Cocoon harvest rate = Number of cocoons harvested / Total number of host plants

[0083] ⑥ The parasitism operation on the second day is the same as on the first day. The transfer tube that introduced 30 pairs of parasitic wasps in step ③ is reconnected to the new parasitism device to start the parasitism on the second day.

[0084] Under these experimental conditions, the parasitism continued until the third day.

[0085] This embodiment is repeated 5 times, and finally the data is processed and analyzed.

[0086] Control 1: Manila lateral groove wasp parasitizing Spodoptera litura A

[0087] (1) The source of the test insects: the parasitic wasp was Manila lateral groove braconid wasp, and the host was beet armyworm.

[0088] (2) Parasite container: Parasite box 1 is a plastic square box with a length of 36cm, a width of 24cm and a height of 10cm; the lid has a square opening with a length of 18cm and a width of 12cm in the middle, and an 80-mesh mesh is attached to the square opening for ventilation.

[0089] (3) Operation process:

[0090] ① Place 300 noctuid moth larvae and an appropriate amount of artificial feed for noctuid moth larvae into parasitic box 1, then place two defatted cotton balls soaked in 10% honey water in the box, and then close and cover parasitic box 1.

[0091] ② Transfer 30 pairs of 3-day-old Manila parasitic wasps from the parasitic wasp rearing box to parasitic box 1. The transfer method is to use a brush to pick up a single parasitic wasp and introduce it into the box through the round opening on the side of parasitic box 1. Finally, plug the round opening with a cotton ball to start the first day of parasitism, which lasts for 24 hours.

[0092] ③ After the parasitism ends on the first day, place parasitism box 1 in a 40*40*40cm insect rearing cage, open its lid to allow 30 pairs of parasitic wasps to fly into the insect rearing cage, and then remove the parasitism container from the insect rearing cage.

[0093] ④ After the parasitism ends, the larvae of the beet armyworm are group-raised in parasitism box 1, and a fixed amount of artificial feed is added daily.

[0094] ⑤ On the 10th to 12th day after the host is parasitized, the bee cocoons are removed from the box and collected into a cocoon collection container. The number of host deaths and parasitism of the noctuid moth larvae, as well as the number of cocoons collected by the parasitic wasps, are counted.

[0095] Host mortality rate = Number of host deaths / Total number of hosts

[0096] Parasite quantity: The number of noctuid moth larvae that are parasitized.

[0097] Parasitism rate = number of parasites / total number of hosts

[0098] Cocoon count: The number of parasitic wasp cocoons formed after the noctuid moth larvae are parasitized.

[0099] Cocoon harvest rate = Number of cocoons harvested / Total number of host plants

[0100] ⑥ The parasitism operation on the second day is the same as on the first day. The 30 pairs of parasitic wasps in the insect rearing cage in step ③ are introduced into the new parasitism box 1 again using a paintbrush to start the parasitism on the second day.

[0101] Under these experimental conditions, the parasitism continued until the third day. This control treatment was repeated five times, and finally, the data were processed and analyzed.

[0102] The experimental results of Control 1 and Example 1 show that: (1) the parasitism rate of the parasitic wasp propagation method in Example 1 is higher than that in Control 1; (2) the cocoon harvesting rate of the parasitic wasp propagation method in Example 1 is much higher than that in Control 1, because the single-head rearing method of the host in its propagation method reduces the number of disease-causing insects in the group rearing process of Spodoptera litura and eliminates the drawback of mutual cannibalism among Spodoptera litura larvae in the group rearing process; (3) the labor time used by the parasitic wasp propagation method in Example 1 is about 1 / 2 of that of the method in Control 1, which greatly reduces the labor cost.

[0103] Table 1 Comparison of propagation effects of Manila parasitic wasps

[0104]

[0105]

[0106] Example 2: Parasitism of the Manila lateral furrow wasp on the beet armyworm B

[0107] (1) The source of the test insects: the parasitic wasp was Manila lateral groove braconid wasp, and the host was the larvae of the beet armyworm.

[0108] (2) Parasite container: The dimensions of the non-transparent bottom box 1 are 42cm long, 30cm wide and 7cm high; the dimensions of the transparent lid square opening are 28cm long and 20cm wide, and 100-mesh mesh is attached to the square opening for ventilation.

[0109] (3) Operation process:

[0110] ① Place 450 early third instar larvae of the beet armyworm in the bottom box of the non-transparent bottom box 1, along with an appropriate amount of blocky artificial feed for the armyworm, and two defatted cotton balls soaked in 10% honey water. Place the transparent cover on top of the bottom box and plug the second round opening on the transparent cover.

[0111] ② Transfer 30 pairs of Manila parasitic wasps that have emerged 3 days ago from the parasitic wasp rearing box to the first transparent transfer tube of the non-transparent bottom box 1. Use the first transparent transfer tube to introduce the parasitic wasps into the non-transparent bottom box 1. After the transfer is completed, plug the first round opening 4 on the side of the non-transparent bottom box 1 to start the first day of parasitism, which lasts for 24 hours.

[0112] ③ After the parasitism ends on the first day, place the non-transparent cover 3 on top of the transparent cover 2, remove the plug on the second round opening 5 and connect the second transparent adapter tube, and introduce all 30 pairs of parasitic wasps into the second adapter tube.

[0113] ④ After the parasitism ends, the larvae of the beet armyworm are transferred to a 36-cell paint box for single-head rearing in one cell. An appropriate amount of artificial feed for armyworms is placed in each cell, and then the lid of the paint box is closed.

[0114] ⑤ On the 10th to 12th day after the host is parasitized, open the lid of the 36-hole paint box, take out the bee cocoons from the holes, collect them into the cocoon collection container, and count the number of host deaths and parasitism of the noctuid moth larvae, as well as the number of cocoons collected by the parasitic wasps.

[0115] Host mortality rate = Number of host deaths / Total number of hosts

[0116] Parasite quantity: The number of noctuid moth larvae that are parasitized.

[0117] Parasitism rate = number of parasites / total number of hosts

[0118] Cocoon count: The number of parasitic wasp cocoons formed after the noctuid moth larvae are parasitized.

[0119] Cocoon harvest rate = Number of cocoons harvested / Total number of host plants

[0120] ⑥ The parasitism operation on the second day is the same as on the first day. The transfer tube that introduced 30 pairs of parasitic wasps in step ③ is reconnected to the new parasitism device to start the parasitism on the second day.

[0121] Under these experimental conditions, the parasitism continued until the third day.

[0122] This embodiment repeats the process three times, and finally performs data processing and analysis.

[0123] Control 2: Manila lateral groove wasp parasitizing Spodoptera litura B

[0124] (1) The source of the test insects: the parasitic wasp was Manila lateral groove braconid wasp, and the host was beet armyworm.

[0125] (2) Parasite container: Parasite box 2 is a plastic square box with a length of 42cm, a width of 30cm and a height of 14cm; the lid has a square opening with a length of 25cm and a width of 18cm in the middle, and a 100-mesh mesh is attached to the square opening for ventilation.

[0126] (3) Operation process:

[0127] ① Place 450 noctuid moth larvae and an appropriate amount of artificial feed for noctuid moth larvae into parasitic box 2, then place two defatted cotton balls soaked in 10% honey water in the box, and then close and cover parasitic box 2.

[0128] ② Transfer 30 pairs of 3-day-old Manila parasitic wasps from the parasitic wasp rearing box to parasitic box 2. The transfer method is to use a brush to pick up a single parasitic wasp and introduce it into the box through the round opening on the side of the parasitic box 2. Finally, plug the round opening with a cotton ball to start the first day of parasitism, which lasts for 24 hours.

[0129] ③ After the parasitism ends on the first day, place parasitism box 2 in a 60*60*60cm insect rearing cage, open its lid to allow 30 pairs of parasitic wasps to fly into the insect rearing cage, and then remove the parasitism container from the insect rearing cage.

[0130] ④ After the parasitism ends, the larvae of the beet armyworm are group-raised in parasitism box 2, and a fixed amount of artificial feed is added daily.

[0131] ⑤ On the 10th to 12th day after the host is parasitized, the bee cocoons are removed from the box and collected into a cocoon collection container. The number of host deaths and parasitism of the noctuid moth larvae, as well as the number of cocoons collected by the parasitic wasps, are counted.

[0132] Host mortality rate = Number of host deaths / Total number of hosts

[0133] Parasite quantity: The number of noctuid moth larvae that are parasitized.

[0134] Parasitism rate = number of parasites / total number of hosts

[0135] Cocoon count: The number of parasitic wasp cocoons formed after the noctuid moth larvae are parasitized.

[0136] Cocoon harvest rate = Number of cocoons harvested / Total number of host plants

[0137] ⑥ The parasitism operation on the second day is the same as on the first day. The 30 pairs of parasitic wasps in the insect rearing cage in step ③ are introduced into the new parasitism box 2 again using a paintbrush to start the parasitism on the second day.

[0138] Under these experimental conditions, the parasitism continued until the third day. The control treatment was repeated three times, and finally, the data were processed and analyzed.

[0139] Under the experimental conditions of increasing the volume of the parasitic device, increasing the number of hosts, and keeping the number of parasitic wasps unchanged, the experimental results of Control 2 and Example 2 show that: (1) the parasitism rate and cocoon harvesting rate in Example 2 are higher than those in Control 2; (2) the labor time required for the parasitic wasp propagation method in Example 2 is about 39% of that in Control 2, which greatly reduces labor costs.

[0140] Table 2 Comparison of propagation effects of Manila parasitic wasps

[0141]

[0142] Example 3: Parasitic armyworms on the varicella wasp

[0143] (1) The source of the test insects: the parasitic wasp was *Sclerotium spp.* and the host was the larvae of armyworms.

[0144] (2) Parasite container: The dimensions of the non-transparent bottom box 1 are 35cm long, 25cm wide and 5cm high; the dimensions of the square opening of the transparent cover 2 are 25cm long and 18cm wide, and an 80-mesh mesh 8 is attached to the square opening for ventilation.

[0145] (3) Operation process:

[0146] ①Because the spotted hymen bee is a parthenogenetic strain, 30 spotted hymen bee cocoons that are about to emerge are placed in the first transparent transfer tube in advance, and a cotton ball soaked in 10% honey water is placed in it.

[0147] ② Place 300 armyworm larvae in the non-transparent bottom box 1, along with an appropriate amount of blocky artificial feed for noctuid moths, and two defatted cotton balls soaked in 10% honey water. Place the transparent cover 2 on top of the bottom box and plug the second round opening 5 on the transparent cover 2.

[0148] ③ Connect 30 molting parasitic wasps that have emerged 3 days ago to the first round opening 4 on the side of the non-transparent bottom box 1 through the first transparent transfer tube, and introduce them into the non-transparent bottom box 1. After the transfer is completed, plug the first round opening 4 and start the first day of parasitism, which lasts for 24 hours.

[0149] ④ After the parasitism ends on the first day, place the non-transparent cover 3 on top of the transparent cover 2, remove the plug on the second round opening 5 of the transparent cover and connect the second transparent adapter tube, and introduce all 30 parasitic wasps into the second transparent adapter tube.

[0150] ⑤ After the parasitic period ends, transfer the armyworm larvae to a 36-cell paint box for single-head rearing. Place an appropriate amount of armyworm artificial feed in each cell, and then close the lid of the paint box.

[0151] ⑥ On the 10th to 12th day after the host is parasitized, open the lid of the 36-hole paint box, take out the bee cocoons from the holes, collect them into the cocoon collection container, and count the number of host deaths and parasitism of armyworm larvae, as well as the number of cocoons collected by parasitic wasps.

[0152] Host mortality rate = Number of host deaths / Total number of hosts

[0153] Parasite quantity: The number of noctuid moth larvae that are parasitized.

[0154] Parasitism rate = number of parasites / total number of hosts

[0155] Cocoon count: The number of parasitic wasp cocoons formed after the noctuid moth larvae are parasitized.

[0156] Cocoon harvest rate = Number of cocoons harvested / Total number of host plants

[0157] ⑦ The parasitism operation on the second day is the same as on the first day. The adapter that introduced 30 parasitic wasps in step ④ is reconnected to the new parasitism device to start the parasitism on the second day.

[0158] Under these experimental conditions, the parasitism continued until the third day. This example was repeated five times, and finally, data processing and analysis were performed.

[0159] Control 3: *Ichthyophthirius multifiliis* parasitized by the armyworm *Ichthyoph

[0160] (1) The source of the test insects: the parasitic wasp was *Sclerotium spp.* and the host was armyworm.

[0161] (2) Parasite container: Parasite box 1 is a plastic square box with a length of 36cm, a width of 24cm and a height of 10cm; the lid has a square opening with a length of 18cm and a width of 12cm in the middle, and an 80-mesh mesh is attached to the square opening for ventilation.

[0162] (3) Operation process:

[0163] ① Place 300 noctuid moth larvae and an appropriate amount of artificial feed for armyworm larvae into parasite box 1, then place two defatted cotton balls soaked in 10% honey water, and cover parasite box 1 with a lid.

[0164] ② Transfer 30 three-day-old spotted parasitic wasps from the parasitic wasp rearing box to parasitic box 1. The transfer method is to use a brush to pick up a single parasitic wasp and introduce it into the box through the round opening on the side of parasitic box 1. Finally, plug the round opening with a cotton ball to start the first day of parasitism, which lasts for 24 hours.

[0165] ③ After the parasitism ends on the first day, place parasitism box 1 in a 40*40*40cm insect rearing cage, open its lid to allow 30 parasitic wasps to fly into the insect rearing cage, and then remove the parasitism container from the insect rearing cage.

[0166] ④ After the parasitism ends, the armyworm larvae are group-raised in parasitism box 1, and a fixed amount of artificial feed is added daily.

[0167] ⑤ On the 10th to 12th day after the host is parasitized, the bee cocoons are removed from the box and collected into a cocoon collection container. The number of host deaths and parasitism of armyworm larvae, as well as the number of cocoons collected by parasitic bees, are counted.

[0168] Host mortality rate = Number of host deaths / Total number of hosts

[0169] Parasite quantity: The number of noctuid moth larvae that are parasitized.

[0170] Parasitism rate = number of parasites / total number of hosts

[0171] Cocoon count: The number of parasitic wasp cocoons formed after the noctuid moth larvae are parasitized.

[0172] Cocoon harvest rate = Number of cocoons harvested / Total number of host plants

[0173] ⑥ The parasitism operation on the second day is the same as on the first day. The 30 parasitic wasps in the insect rearing cage in step ③ are introduced into the new parasitism box 1 again using a paintbrush to start the parasitism on the second day.

[0174] Under these experimental conditions, the parasitism continued until the third day. This control treatment was repeated five times, and finally, the data were processed and analyzed.

[0175] The experimental results of Control 3 and Example 3 show that: (1) the parasitism rate of the parasitoid wasp propagation method in Example 3 is higher than that in Control 3; (2) the cocoon harvesting rate of the parasitoid wasp propagation method in Example 3 is higher than that in Control 3, because the single-head rearing method of the host in its propagation method reduces the number of larvae infected during the group rearing process, and also avoids the problem of cannibalism among larvae and the consumption of cocoons during the group rearing process; (3) the labor time required for the propagation method of parasitoid wasp in Example 3 is about 45% of that in Control 3, which significantly reduces labor costs.

[0176] Table 3 Comparison of propagation effects of *Sargassum fusiforme*

[0177]

[0178] The above description is merely an embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structural or procedural transformations made based on the content of the present invention specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of the present invention.

Claims

1. A device for propagating parasitic wasps larvae, characterized in that, The device includes a non-transparent base box, a transparent cover, and a non-transparent cover arranged sequentially from bottom to top and capable of being sealed together. The non-transparent base box is open, and a first inlet connected to its internal cavity is provided on its side wall. The transparent cover has a second inlet corresponding to the internal cavity, such that when the transparent cover is placed over the opening of the non-transparent base box, the second inlet is connected to the internal cavity. The non-transparent cover has a third inlet corresponding to the second inlet. The device also includes a first plug capable of sealing the first inlet and a first transparent adapter body detachably connected to the first inlet, as well as a second plug capable of sealing the second inlet. A second transparent adapter body that can pass through the third inlet and is detachably connected to the second inlet; the first transparent adapter body is a first transparent adapter pipe, the second transparent adapter body is a second transparent adapter pipe, and the first transparent adapter pipe and the second transparent adapter pipe are a shared transparent adapter pipe; the first inlet is a first circular opening, the second inlet is a second circular opening, the third inlet is a third circular opening, and the opposite ends of the shared transparent adapter pipe are a first end and a second end, respectively, the first end is used to connect to the first circular opening, the second end is used to connect to the second circular opening, the first plug is a first circular opening plug, and the second plug is a second circular opening plug.

2. The device for propagating parasitic wasps larvae according to claim 1, characterized in that, The first end is shaped like a round opening matching the first round opening, and a rubber ring is fitted over it to seal and connect to the first round opening. A through hole is provided on the transparent cover, and an upwardly protruding hollow ring is provided at the edge of the through hole. The through hole and the internal channel of the hollow ring form the second round opening. Two opposing arc-shaped snap-fit ​​protrusions are provided on the outer tube wall of the second end. Two opposing arc-shaped snap-fit ​​grooves are provided on the inner side wall of the hollow ring, corresponding to the two arc-shaped snap-fit ​​protrusions, so that the two arc-shaped snap-fit ​​protrusions of the second end can be inserted into the second round opening and can be rotated and snapped into the two arc-shaped snap-fit ​​grooves. The third round opening of the non-transparent cover can be fitted onto the outer periphery of the hollow ring.

3. The device for propagating parasitic wasps larvae according to claim 2, characterized in that, The lower end of the hollow ring is detachably connected to the transparent cover by an adhesive bonding method.

4. The device for propagating parasitic wasps larvae according to claim 2, characterized in that, The shared transparent adapter pipe is in the form of a bend.

5. The device for propagating parasitic wasps larvae according to claim 2, characterized in that, The first and second circular openings are the same size.

6. The device for propagating parasitic wasps larvae according to claim 1, characterized in that, The first round plug and the second round plug are a common round plug.

7. The device for propagating parasitic wasps larvae according to claim 1, characterized in that, The non-transparent bottom box, the transparent cover, and the non-transparent cover are connected in sequence by snap-fit ​​connections.

8. The device for propagating parasitic wasps larvae according to claim 1, characterized in that, The dimensions of the non-transparent bottom box are: length 20-60cm, width 15-50cm, and height 4-10cm.

9. A method for breeding parasitic wasps larvae, characterized in that, The breeding of parasitic wasps using the apparatus for propagating larval parasitic wasps according to any one of claims 1-8 includes the following: Place a measured amount of host of appropriate age and an appropriate amount of host feed in the non-transparent bottom box, add a cotton ball soaked in honey water, place the transparent cover on the non-transparent bottom box, and plug the second round opening on the transparent cover with the second round opening plug. Remove the first round plug from the first round opening, connect the first transparent adapter tube, introduce the parasitic wasp inside the transparent adapter tube into the non-transparent bottom box, and finally close the first round opening with the first round plug. In an insect rearing chamber under certain conditions, parasitic wasps complete the parasitism process within the device. After the parasitic process is complete, place the non-transparent cap on top of the transparent cap; Remove the second round plug from the transparent cap and connect the second transparent adapter tube, then introduce the parasitic wasp into the second transparent adapter tube.

10. The method for breeding parasitic wasps larvae according to claim 9, characterized in that, It also includes the following steps: Host rearing after parasitism: Single-head rearing method is adopted. The container for single-head rearing is a 32-hole rearing tray. Each hole is filled with an appropriate amount of artificial feed for the host. The host in the non-transparent bottom box is transferred to the 32-hole feed tray, one host per hole. The tray is placed on the shelf of the insect rearing room for rearing. Harvesting bee cocoons: On the 10th-11th day after parasitism, open the breathable sealing film of the 32-hole tray, take out the bee cocoons from the holes, and collect them into the cocoon collection container. When the bee cocoons are about to emerge, transfer them to the parasitic bee rearing round box or the first transparent transfer tube, provide honey water, and wait for them to emerge for use.

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