A plant composition with penetration-promoting efficacy, and a preparation method and application thereof

Abstract

The application discloses a plant composition with penetration promoting efficacy, and raw materials of active ingredients are as follows: 0.5-1 parts of Schinus terebinthifolius, 13-18 parts of Mentha spicata, and 5-10 parts of ginger root.The plant composition with penetration promoting efficacy has the characteristics of remarkable effect, weak smell and no irritation, solves the deficiency of traditional penetration promoters in cosmetic application, and through the efficacy synergy among multiple components, the diffusion coefficient of drugs is increased, the penetration and diffusion of drugs to the deep layer of skin are promoted, the bioavailability of efficacy materials is improved, the efficacy materials reach the target position, and the efficacy of whitening, soothing, anti-aging, acne-removing and other active substances in cosmetics is improved, the irritation of chemical synthetic penetration promoters to skin can be avoided, the use safety of products is ensured, and the plant composition has a wide development prospect.

Description

Technical Field

[0001] This invention relates to a natural extract, specifically to a plant composition with enhanced penetration properties, its preparation method, and its application. Background Technology

[0002] The skin, the largest organ in the human body, acts as both a chemical and physical barrier, and is the primary barrier to the transdermal absorption of foreign substances. Various active ingredients in cosmetics must be absorbed through the skin to enter the body. However, among all skin layers, the stratum corneum has the strongest barrier effect. Its brick-wall structure, composed of stratum corneum cells and intercellular lipids, hinders the transdermal absorption of various active ingredients in cosmetics. Because most cosmetic active ingredients have poor transdermal permeability, the penetration rate and amount are insufficient to meet the effective dosage requirements. Consequently, the active ingredients in cosmetics rarely penetrate the skin to reach the body and exert their intended effects. Therefore, increasing the amount of active ingredients that penetrate the skin is crucial for transdermal drug delivery systems.

[0003] Currently, cosmetic penetration enhancement technologies mainly include physical methods such as iontophoresis, ultrasound iontophoresis, electroporation, and microneedling. However, most of these technologies are still in the laboratory research stage, and due to the poor portability and safety of the instruments, there are no commercially viable products on the market. Chemical penetration enhancers remain the most widely used and convenient method, commonly including dimethyl sulfoxide, laurocapram, isosorbide dimethyl ether, sodium tetradecyl sulfate, and dimethylformamide. However, there are still no completely ideal chemical penetration enhancers. Most chemical transdermal absorption enhancers have poor biocompatibility or are highly irritating, and even some relatively ideal chemical penetration enhancers exhibit toxicity with increasing concentration. On the other hand, the effectiveness of chemical penetration enhancers is highly dose-dependent, thus creating a contradiction between adverse drug reactions and the drug's penetration-enhancing effects.

[0004] Existing penetration enhancers are mainly oil-soluble substances with a strong odor, resulting in drawbacks in cosmetic applications such as low solubility, unpleasant smells, and toxic side effects. Compared to chemical penetration enhancers, traditional Chinese medicine transdermal absorption enhancers are less irritating to the skin, have lower toxicity, and can also produce synergistic effects with drugs.

[0005] Therefore, there is an urgent need to develop a safe, non-irritating, natural plant-derived, effective, and stable high-efficiency transdermal absorption enhancer to address the shortcomings of existing technologies. Summary of the Invention

[0006] The purpose of this invention is to overcome at least one deficiency of the prior art and to provide a plant composition with a penetration-enhancing effect and its application.

[0007] The technical solution adopted in this invention is:

[0008] In the first aspect, the present invention provides a plant composition with a penetration-enhancing effect, wherein the active ingredients are pepper fruit, peppermint, and ginger root.

[0009] In some instances, the active ingredient is an aqueous extract of the raw material.

[0010] In some examples, the raw materials of the active ingredient are in the following mass ratios: 0.5-1 part of Sichuan pepper fruit, 13-18 parts of peppermint, and 5-10 parts of ginger root.

[0011] In some instances, the raw materials of the active ingredient are proportioned as follows: 0.8 parts of Sichuan pepper fruit, 16 parts of peppermint, and 8 parts of ginger root.

[0012] In some instances, the method for preparing the plant composition includes the following steps:

[0013] 1) Weigh out the pepper fruit, peppermint, and ginger root according to the proportion, grind each herb into powder and mix evenly to obtain a compound herbal powder.

[0014] 2) Add 20 to 40 times the weight of deionized water to the compound plant powder obtained in step 1 for water extraction, then centrifuge and collect the clear liquid to obtain the compound plant extract.

[0015] 3) The plant compound extract collected in step 2 was separated using a ceramic membrane with a pore size of 200-500 nm, purified using an ultrafiltration membrane with a pore size of 5000-8000 dal, and concentrated using a nanofiltration membrane with a pore size of 100-200 dal until the relative density was 1.06-1.16, thus obtaining the concentrate.

[0016] 4) Add 1 to 3 times the amount of extractant to the concentrate by weight, stir and disperse evenly, let stand for 24 to 36 hours, centrifuge and collect the clear liquid to obtain the plant composition with the aforementioned penetrating effect.

[0017] In some instances, the water extraction speed in step 2) is 60–90 rpm, the water extraction temperature is 25–65°C, and the water extraction time is 60–120 min.

[0018] In some instances, the extractant in step 4) is selected from at least one of C3-C4 diols.

[0019] In a second aspect, the present invention provides a penetration enhancer comprising excipients and an active ingredient, wherein the active ingredient is a plant composition as described in any one of claims 1 to 7.

[0020] In some instances, the excipients include at least one of salicylic acid, lauryl ketone, tetrahydropiperine, propylene glycol, and borneol.

[0021] Thirdly, the application of the penetration enhancer described in the second aspect of the present invention in the preparation of cosmetics, wherein the mass addition amount of the penetration enhancer is 0.1-10%.

[0022] The beneficial effects of this invention are:

[0023] This invention provides a plant composition with enhanced penetration effects, characterized by significant efficacy, mild odor, and gentle safety, overcoming the shortcomings of traditional penetration enhancers in cosmetic applications. This plant composition with enhanced penetration effects is derived from plant extracts. Specifically, the alkaloids and amides in Sichuan pepper fruit promote blood circulation, increasing blood flow within the skin's blood vessels and facilitating the diffusion of active ingredients from the epidermis to the dermis. The menthol and menthone in peppermint have a strong affinity for cholesterol in the lipid layer, increasing the fluidity of the stratum corneum lipid bilayer by acting on the A and B chains of ceramides and free fatty acid molecules, thus improving the permeability of active substances. The gingerol and gingerol in ginger root alter the microstructure of the stratum corneum, increasing the interlayer porosity and promoting transdermal penetration of active substances, thereby enhancing the skin's storage capacity. The present invention provides a plant composition with penetration-enhancing effects. Through the synergistic effect of multiple components, it increases the diffusion coefficient of the active ingredients, promotes the penetration and diffusion of the active ingredients into the deep layers of the skin, and helps to improve the bioavailability of the active ingredients. This allows the active ingredients to reach the target location, thereby enhancing the whitening, soothing, anti-aging, and acne-removing effects of cosmetics. It avoids the skin irritation caused by chemically synthesized penetration enhancers, ensures the safety of product use, and has broad development prospects. Detailed Implementation

[0024] The following disclosure provides many different implementations or examples for different ways of implementing the present invention.

[0025] Sichuan pepper fruit, also known as zanthoxylum bungeanum Maxim., is the mature fruit of this plant, belonging to the Rutaceae family. It is warm in nature, pungent in taste, and enters the spleen, stomach, and kidney meridians. It possesses medicinal properties including dispelling wind and pathogens, reducing swelling and relieving pain, warming the middle jiao (spleen and stomach) to relieve pain, killing parasites and relieving itching, and invigorating the spleen and stomach. Sichuan pepper fruit can dilate blood vessels, promote blood circulation, and increase blood flow in the skin's blood vessels, thereby accelerating the body's absorption and metabolism.

[0026] Spicy peppermint is the dried aerial part of the plant *Mentha piperita* Linn., belonging to the Lamiaceae family. It is pungent and cool in nature. It enters the lung and liver meridians. It disperses wind-heat, clears the head and eyes, soothes the throat, promotes rash eruption, and soothes the liver and regulates qi. It is used for wind-heat colds, early stages of wind-heat syndrome, headache, red eyes, sore throat, mouth ulcers, urticaria, measles, and chest and rib distension. It also has expectorant, anti-inflammatory, analgesic, anti-tumor, central nervous system stimulant, and absorption-promoting effects.

[0027] Ginger root, the rhizome of the ginger plant (Zingiber officinale Rosc.), is pungent and slightly warm in nature, and enters the lung, spleen, and stomach meridians. It relieves exterior syndromes and dispels cold, warms the middle jiao and stops vomiting, resolves phlegm and stops coughing, and detoxifies fish and crab poisoning. It is used for common cold due to wind-cold, vomiting due to stomach cold, cough with cold phlegm, and fish and crab poisoning.

[0028] Clove is the dried flower bud of the clove (Eugenia caryophyllata Thunb.), a plant in the Myrtaceae family. It is pungent and warm in nature. It enters the spleen, stomach, lung, and kidney meridians. It warms the middle jiao (spleen and stomach) and relieves nausea and vomiting, tonifies the kidneys and strengthens yang. It is used for spleen and stomach deficiency-cold, hiccups and vomiting, poor appetite and diarrhea, cold pain in the heart and abdomen, and kidney deficiency-induced impotence. Its volatile oils and eugenol, among other substances, have antibacterial, anti-inflammatory, analgesic, and strong penetrating properties.

[0029] The mass fractions of each plant material in Examples 1-8 and Comparative Examples 1-7 of this invention are shown in Table 1 below. In Comparative Example 7, the plant material peppermint was replaced with clove, which has a similar function. The preparation methods for Examples 1-8 are described below.

[0030] Table 1

[0031] Qin pepper fruit / serving Spicy Mint / Serving Ginger root / serving Example 1 0.8 16 8 Example 2 0.5 13 10 Example 3 1 18 5 Example 4 0.6 15 7 Example 5 0.9 17 6 Example 6 0.8 16 8 Example 7 0.8 16 8 Example 8 0.8 16 8 Comparative Example 1 0.8 0 0 Comparative Example 2 0 16 0 Comparative Example 3 0 0 8 Comparative Example 4 0.8 16 0 Comparative Example 5 0 16 8 Comparative Example 6 0.8 0 8 Comparative Example 7 0.8 16 8

[0032] Example 1

[0033] The preparation method of the plant composition with enhanced penetration effect in this embodiment includes the following steps:

[0034] Step 1: Weigh out 0.8 parts of Sichuan pepper fruit, 16 parts of peppermint, and 8 parts of ginger root. Grind each herb into 80 mesh, mix them evenly, and obtain the compound herbal powder.

[0035] Step 2: Place the plant compound herbal powder obtained in Step 1 into a Chinese herbal medicine directional extraction device, add 30 times the weight of deionized water, stir at 80 rpm, extract at 30℃, and extract for 90 min; the obtained extract is centrifuged and the clear liquid is collected to obtain the plant compound extract.

[0036] Step 3: The plant compound extract collected in Step 2 was separated using a ceramic membrane with a pore size of 300 nm, purified using an ultrafiltration membrane with a pore size of 6000 dal, and concentrated using a nanofiltration membrane with a pore size of 100 dal, with the specific gravity of the concentrate controlled at 1.06.

[0037] Step 4: Add 2 times the amount of 1,3-butanediol to the concentrate by weight, stir and disperse evenly, let stand for 24 hours, centrifuge to separate, and the clear liquid obtained is a plant composition with the function of promoting penetration.

[0038] Example 2

[0039] The preparation method of the plant composition with enhanced penetration effect in this embodiment includes the following steps:

[0040] Step 1: Weigh out 0.5 parts of Sichuan pepper fruit, 13 parts of peppermint, and 10 parts of ginger root. Grind each herb into 100 mesh, mix them evenly, and obtain the compound herbal powder.

[0041] Step 2: Place the plant compound herbal powder obtained in Step 1 into a Chinese herbal medicine directional extraction device, add 20 times the weight of deionized water, stir at 80 rpm, extract at 65℃, and extract for 120 min; the obtained extract is centrifuged and the clear liquid is collected to obtain the plant compound extract.

[0042] Step 3: Separate the plant compound extract collected in Step 2 using a ceramic membrane with a pore size of 200 nm, purify it using an ultrafiltration membrane with a pore size of 6000 dal, and concentrate it using a nanofiltration membrane with a pore size of 200 dal, controlling the specific gravity of the concentrate to be 1.09.

[0043] Step 4: Add 1.5 times the amount of 1,2-propanediol to the concentrate by weight, stir and disperse evenly, let stand for 30 hours, centrifuge to separate, and obtain the clear liquid, which is a plant composition with the function of promoting penetration.

[0044] Example 3

[0045] The preparation method of the plant composition with enhanced penetration effect in this embodiment includes the following steps:

[0046] Step 1: Weigh out 1 part of Sichuan pepper fruit, 18 parts of peppermint, and 5 parts of ginger root. Grind each herb into 60 mesh, mix them evenly, and obtain the compound herbal powder.

[0047] Step 2: Place the plant compound herbal powder obtained in Step 1 into a Chinese herbal medicine directional extraction device, add 40 times the weight of deionized water, stir at 80 rpm, extract at 40℃, and extract for 60 min; the obtained extract is centrifuged and the clear liquid is collected to obtain the plant compound extract.

[0048] Step 3: The plant compound extract collected in Step 2 was separated using a ceramic membrane with a pore size of 500 nm, purified using an ultrafiltration membrane with a pore size of 8000 dal, and concentrated using a nanofiltration membrane with a pore size of 120 dal, with the specific gravity of the concentrate controlled at 1.16.

[0049] Step 4: Add 3 times the amount of 1,4-butanediol to the concentrate by weight, stir and disperse evenly, let stand for 24 hours, centrifuge to separate, and obtain the clear liquid, which is a plant composition with the function of promoting penetration.

[0050] Example 4

[0051] The preparation method of the plant composition with enhanced penetration effect in this embodiment includes the following steps:

[0052] Step 1: Weigh out 0.6 parts of Sichuan pepper fruit, 15 parts of peppermint, and 7 parts of ginger root. Grind each herb into 60 mesh, mix them evenly, and obtain the compound herbal powder.

[0053] Step 2: Place the plant compound herbal powder obtained in Step 1 into a Chinese herbal medicine directional extraction device, add 20 times the weight of deionized water, stir at 90 rpm, extract at 25℃, and extract for 60 min; the obtained extract is centrifuged and the clear liquid is collected to obtain the plant compound extract.

[0054] Step 3: Separate the plant compound extract collected in Step 2 using a ceramic membrane with a pore size of 200 nm, purify it using an ultrafiltration membrane with a pore size of 8000 dal, and concentrate it using a nanofiltration membrane with a pore size of 100 dal, controlling the specific gravity of the concentrate to be 1.13.

[0055] Step 4: Add 1.5 times the amount of 1,3-propanediol to the concentrate by weight, stir and disperse evenly, let stand for 36 hours, centrifuge to separate, and the clear liquid obtained is a plant composition with the function of promoting penetration.

[0056] Example 5

[0057] The preparation method of the plant composition with enhanced penetration effect in this embodiment includes the following steps:

[0058] Step 1: Weigh out 0.9 parts of Sichuan pepper fruit, 17 parts of peppermint, and 6 parts of ginger root. Grind each herb into 100 mesh, mix them evenly, and obtain the compound herbal powder.

[0059] Step 2: Place the plant compound herbal powder obtained in Step 1 into a Chinese herbal medicine directional extraction device, add 40 times the amount of deionized water by weight, stir at 60 rpm, extract at 50℃, and extract for 120 min; the obtained extract is centrifuged and the clear liquid is collected to obtain the plant compound extract.

[0060] Step 3: Separate the plant compound extract collected in Step 2 using a ceramic membrane with a pore size of 500 nm, purify it using an ultrafiltration membrane with a pore size of 5000 dal, and concentrate it using a nanofiltration membrane with a pore size of 200 dal, controlling the specific gravity of the concentrate to be 1.080.

[0061] Step 4: Add 2.0 times the weight of butanediol to the concentrate, stir and disperse evenly, let stand for 24 hours, centrifuge to separate, and obtain the clear liquid, which is a plant composition with the function of promoting penetration.

[0062] Example 6

[0063] The preparation method of the plant composition with enhanced penetration in this embodiment is the same as in Example 1, except that in step 4, 0.5 times the amount of 1,3-butanediol is added to the concentrate, stirred and dispersed evenly, and allowed to stand for 12 hours.

[0064] Example 7

[0065] The method for preparing the plant composition with enhanced penetration in this embodiment is as follows: 2% tetrahydropiperine is added to the plant composition obtained in Example 1.

[0066] Example 8

[0067] The method for preparing the plant composition with enhanced penetration in this embodiment is as follows: 2% lauryl ketone is added to the plant composition obtained in Example 1.

[0068] The preparation methods of the traditional Chinese medicine compositions in Comparative Examples 1 to 7 are the same as those in Example 1.

[0069] Take the plant compositions obtained in Examples 1-8 and Comparative Examples 1-7, and prepare the corresponding skin care essences according to the formulas in Table 2.

[0070] Table 2

[0071]

[0072] To prepare the A-phase solution, the A-phase powder from Table 2 was dispersed evenly in butylene glycol, then added to deionized water while stirring. The water was heated to approximately 85°C until completely dissolved and homogeneous into a transparent liquid. This mixture was kept at this temperature for 20 minutes for sterilization, and then cooled to below 45°C to obtain the A-phase solution. Pre-dissolved and homogeneous components of phases B and C were added to the A-phase solution and stirred until dissolved to obtain the skincare essence. Skincare essence products from each example and comparative example were prepared using the same method, and then the penetration-enhancing efficacy of each active ingredient and the safety assessment of its irritation were performed.

[0073] Permeation-enhancing performance test of permeation-enhancing active ingredients

[0074] Human ex vivo skin is an ideal medium for permeation enhancement studies; however, due to significant differences in skin structure between different donors or different sites, and ethical constraints, animal skin differs greatly from human ex vivo skin in morphological characteristics, water content, and stratum corneum lipid composition—factors crucial to skin barrier function. In contrast, 3D full-thickness skin models, derived from human cells and grown under the same conditions, possess advantages such as morphological and structural characteristics, lipid composition and arrangement, and barrier function against external substances that more closely resemble natural human skin, thus increasing the stability and reliability of experimental results.

[0075] The penetration-enhancing performance test used a 3D full-thickness skin model as a carrier, and the test was conducted in accordance with GB / T27818-2011 standard for in vitro testing methods of chemical skin absorption. The penetration-enhancing ability of the skincare serums prepared in the above examples and comparative examples was tested using a Franz vertical diffusion cell. The blank group consisted of phase B of the skincare serum, which did not contain any of the penetration-enhancing active ingredients from the examples and comparative examples. The test procedures and results are as follows.

[0076] (1) Fix the 3D skin between the upper and lower chambers of the Franz diffusion cell, with the epidermal layer facing the drug delivery cell and the dermal layer facing the receiving chamber. Add 0.5 mL of receiving solution (physiological saline) using a pipette to ensure close contact between the dermal layer and the receiving solution to expel air. The total volume of the receiving solution is 7.5 mL.

[0077] (2) Fix the Franz diffusion cell in a constant temperature water bath, turn on the electromagnetic stirrer to stir at a speed of 300 rpm / min, maintain a constant temperature water bath of 36.5℃, and ensure that there are no air bubbles in the water bath jacket.

[0078] (3) After the water bath temperature stabilizes, accurately pipette a fixed volume of 400 μl of skin care product sample and evenly apply it to the skin surface in the supply chamber. Remove the 3D skin at 8 hours, and use a syringe to collect a sample from the receiving chamber. After filtering the sample through a 0.2 μm microporous membrane, analyze the content of the active ingredient ectoine using high performance liquid chromatography (HPLC), and calculate the 8-hour diffusion rate P using the following formula.

[0079]

[0080] In the formula: P is the diffusion percentage over 8 hours, P t P0 is the sample content in the receiving cell after 8 hours, and P0 is the initial sample content in the supply cell.

[0081] The percentage of penetration of the skin care essence prepared in each embodiment, comparative example, and blank group at 8 hours is shown in Table 3.

[0082] Table 3

[0083]

[0084]

[0085] The results of the 8-hour penetration percentage test show that, compared with the blank group, Examples 1-5 significantly improved the diffusion rate of the active ingredient ectoine, with an average penetration increase of 24.08%.

[0086] The test results of Example 6 show that when the preparation process parameters are not within the scope of the present invention, the penetration-enhancing effect is significantly reduced, but still higher than that of the comparative examples.

[0087] The test results from Examples 7 and 8 show that when the plant composition of the present invention is combined with tetrahydropiperine or water-soluble lauryl acetone, its penetration-enhancing effect is improved to a certain extent.

[0088] The test results of Comparative Examples 1 to 6 show that using one or two kinds of medicinal materials does not significantly improve the diffusion rate of the active ingredient ectoine.

[0089] The test results of Comparative Example 7 show that when the raw materials of the active ingredients are replaced with Chinese medicinal herbs with similar functions, the penetration-enhancing effect will be significantly reduced. At the same time, the skin care essence prepared in this comparative example has a strong odor, which affects the consumer's user experience.

[0090] The 8-hour penetration percentage test results of the examples and comparative examples show that the plant composition of the present invention has a significant penetration-promoting effect and a clear synergistic effect under specific raw material combinations and preparation process conditions.

[0091] Irritation safety assessment of permeation-enhancing active ingredients

[0092] Cosmetics are daily consumer products that come into direct, long-term contact with human skin, making the safety of cosmetic materials a crucial aspect of ensuring consumer safety. Currently, most countries worldwide have passed laws prohibiting animal testing for cosmetics. The chicken embryo chorioallantoic membrane (CAM) test is one of the earliest adopted in vitro irritation assessment methods. The chorioallantoic membrane is a respiratory membrane surrounding the chicken embryo. Utilizing the intact, clear, and transparent vascular system of the mid-stage hatched chicken embryo's chorioallantoic membrane, a certain amount of the test substance is directly exposed to the membrane. After a period of time, changes in chorioallantoic membrane toxicity indicators (such as hemorrhage, coagulation, and vascularization) are observed. These indicators reflect changes in the morphology, color, and permeability of blood vessels and vascular networks, as well as phenomena such as chorioallantoic membrane protein denaturation and the degree of damage. These results are then combined to obtain a score used to assess the irritant properties of the test substance.

[0093] The irritation safety test of the penetration-enhancing active ingredient was conducted according to GB / T27818-2011, the standard for in vitro tests on skin absorption of chemicals. A 0.9% sodium chloride solution was used as the negative control, and a 1% SDS solution was used as the positive control. 0.3 ml of the test substance was applied to cover the CAM surface, and the CAM reaction was observed immediately after application for 5 minutes, with the results recorded. Bleeding, coagulation, and vascularization were graded and scored according to their severity; the grading and scoring criteria are shown in Table 4.

[0094] Table 4

[0095] 0 points 1 point 2 points 3 points Bleeding No bleeding Mild bleeding Moderate bleeding Severe bleeding Blood clotting No clotting Mild coagulation Moderate coagulation Severe coagulation blood vessel dissolution Angiolysis Mild angiogenesis moderate vessel dissolution severe vasolysis

[0096] The stimulus score (IS) is calculated using the following formula, and the result is rounded to two decimal places.

[0097]

[0098] In the formula: sec H: the average time for the onset of bleeding observed on the CAM membrane, in seconds (s); sec L: the average time for the onset of vascular dissolution observed on the CAM membrane, in seconds (s); sec C: the average time for the onset of coagulation observed on the CAM membrane, in seconds (s).

[0099] Based on the calculated IS values, the irritant properties of the test substances are classified according to Table 5.

[0100] Table 5

[0101] Stimulus rating Irritant Classification IS<1 Non-irritating 1≤IS<5 Mild irritation 5≤IS<9 moderate irritation IS≥10 Strongly irritating / corrosive

[0102] The irritation test results of the skin care essences prepared in each embodiment and comparative example, as well as the negative control group and positive control group, are shown in Table 6.

[0103] Table 6

[0104] sample IS value Irritant Classification negative control 0.57 Non-irritating Positive control 12.63 Strong irritant Example 1 0.62 Non-irritating Example 2 0.73 Non-irritating Example 3 0.67 Non-irritating Example 4 0.72 Non-irritating Example 5 0.69 Non-irritating Example 6 0.58 Non-irritating Example 7 0.92 Non-irritating Example 8 2.67 Mild irritation Comparative Example 1 0.61 Non-irritating Comparative Example 2 0.63 Non-irritating Comparative Example 3 0.65 Non-irritating Comparative Example 4 0.69 Non-irritating Comparative Example 5 0.69 Non-irritating Comparative Example 6 0.87 Non-irritating Comparative Example 7 0.82 Mild irritation

[0105] The response results of the negative and positive control groups in the irritant test fell within the categories of non-irritant and strongly irritant, respectively, indicating that the test results were acceptable.

[0106] As can be seen from the results of the irritation tests in Examples 1 to 6, the plant compositions of the present invention with the effect of promoting penetration are all non-irritating.

[0107] As can be seen from the results of the irritation test in Example 7, tetrahydropiperine is not irritating;

[0108] The results of the irritation test in Example 8 show that water-soluble laurocapram has mild irritation.

[0109] The results of the irritation tests of Comparative Examples 1 to 6 show that the medicinal materials used in this invention are all non-irritating.

[0110] The results of the irritation experiment in Comparative Example 7 show that replacing the plant-based raw material peppermint with cloves, which have similar functions, results in a certain degree of mild irritation.

[0111] The present invention provides a plant-based composition with enhanced skin penetration, derived from plant extracts. This avoids the skin irritation caused by chemically synthesized penetration enhancers, ensuring the safety of the product. Furthermore, it has no unpleasant odor and does not affect the user experience of cosmetic consumers.

[0112] The above is a further detailed description of the present invention and should not be considered as a limitation on the specific implementation of the present invention. For those skilled in the art, simple deductions or substitutions without departing from the concept of the present invention are all within the protection scope of the present invention.

Claims

1. A plant composition with penetrating power, characterized in that, The active ingredients are derived from Sichuan pepper fruit, peppermint, and ginger root. The mass ratio of the active ingredients is: Sichuan pepper fruit 0.5-1 part, peppermint 13-18 parts, and ginger root 5-10 parts. The preparation method of the plant composition includes the following steps: 1) Weigh out the pepper fruit, peppermint, and ginger root according to the proportion, grind each herb into powder and mix evenly to obtain a compound herbal powder. 2) Add 20 to 40 times the weight of deionized water to the plant compound powder obtained in step 1 for water extraction. The water extraction speed is 60 to 90 rpm, the water extraction temperature is 25 to 65℃, and the water extraction time is 60 to 120 min. Then centrifuge and collect the clear liquid to obtain the plant compound extract. 3) The plant compound extract collected in step 2 is separated by ceramic membrane with a pore size of 200-500 nm, purified by ultrafiltration membrane with a pore size of 5000-8000 dal, and concentrated by nanofiltration membrane with a pore size of 100-200 dal. The relative density of the concentrated solution is controlled to be 1.06-1.16 to obtain the concentrated solution. 4) Add 1 to 3 times the amount of extractant by weight to the concentrate. The extractant is selected from at least one of C3 to C4 diols. Stir and disperse evenly, let stand for 24 to 36 hours, centrifuge and collect the clear liquid to obtain the plant composition with the aforementioned penetrating effect.

2. The plant composition according to claim 1, characterized in that, The mass ratio of the raw materials of the active ingredient is as follows: 0.8 parts of Sichuan pepper fruit, 16 parts of peppermint, and 8 parts of ginger root.

3. A penetration enhancer, comprising excipients and an active ingredient, characterized in that, The active ingredient is the plant composition described in claim 1 or 2.

4. The penetration enhancer according to claim 3, characterized in that, The excipients include at least one of salicylic acid, lauryl ketone, tetrahydropiperine, and borneol.

5. The application of the penetration enhancer according to claim 3 or 4 in the preparation of cosmetics, characterized in that, The mass addition amount of the penetration enhancer is 0.1% to 10%.

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