A spindle-shaped lysinibacillus and its application

Abstract

The invention discloses a spindle-shaped lysinibacillus and an application thereof, and relates to the field of microbial screening and application. The deposit number of the spindle-shaped lysinibacillus is CGMCC No. 28950. The spindle-shaped lysinibacillus provided by the invention has the properties of dissolving phosphorus and potassium, promoting the growth of crops, and having a good control effect on southern root-knot nematodes.

Description

Technical Field

[0001] The invention relates to the field of microbial screening and application, and in particular to a lysine bacillus fusiformis and application thereof. Background Art

[0002] In recent years, plant parasitic nematodes have become one of the important factors restricting the development of the global greenhouse vegetable industry. Among them, root-knot nematode disease is one of the most common and destructive soil-borne diseases in greenhouse vegetable continuous cropping, causing huge losses to the greenhouse vegetable industry. Many studies have been conducted on the prevention and control measures of root-knot nematodes, with the focus on breeding resistant plant varieties and developing nematicides. Breeding resistant plant varieties can effectively reduce the losses caused by nematodes, but due to the severe selection pressure on resistant plants, the invasion of many other pathogens will lead to weakened resistance. Nematicides can effectively control nematodes, but their toxic residues affect the environment and human health. In order to achieve green vegetable production, most nematicides are gradually being banned or strictly restricted. Therefore, there is an urgent need to develop alternative control strategies and long-term integrated approaches to protect against nematodes to replace chemical nematicides.

[0003] Biological control has attracted attention because it does not damage the agricultural ecological environment and avoids the harm of pesticides to human health. At present, the root-knot nematode antagonistic microorganisms commonly used in biological control are mostly screened from crop soils infected by root-knot nematodes, while there are relatively few studies on the isolation and screening of root-knot nematode antagonistic bacteria from organic fertilizers. Organic fertilizers based on organic waste, such as livestock and poultry manure, straw, and green manure, have become a research hotspot at this stage because of their many advantages such as low cost, easy availability, improvement of soil environment, control of soil-borne diseases, promotion of plant growth, waste recycling, and reduction of environmental pollution.

[0004] The fusiform lysinibacillus provided by the present invention has the performance of dissolving phosphorus and potassium, promotes the growth of crops, and has a good control effect on southern root-knot nematodes. Summary of the invention

[0005] The purpose of the present invention is to provide a spindle-shaped lysine bacillus and application thereof to solve the problems existing in the prior art.

[0006] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0007] The invention discloses a spindle-shaped lysinobacil. The spindle-shaped lysinobacil is classified as spindle-shaped lysinobacil and has been deposited in the General Microbiological Center of China Microbiological Culture Collection Committee with a deposit number of CGMCC No.28950.

[0008] Compared with the prior art, the beneficial effects achieved by the present invention are:

[0009] The present invention provides a spindle-shaped lysinibacillus, which is found through experimental detection to have an excellent control effect on southern root-knot nematodes and can dissolve phosphorus and potassium in the soil to promote the growth of crops. The spindle-shaped lysinibacillus is used as an antagonistic bacterium to southern root-knot nematodes and applied to agricultural production, so that waste can be reused, and the damage to the environment caused by chemical pesticide residues is avoided, which is green and environmentally friendly.

[0010] The present invention also provides the use of the fusiform lysinibacillus in treating southern root-knot nematodes.

[0011] The invention also provides application of the spindle-shaped lysine bacillus in producing protease and chitinase.

[0012] The invention also provides application of the fusiform lysinibacillus in dissolving phosphorus and potassium. DETAILED DESCRIPTION

[0013] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0014] Test Example 1

[0015] Physiological and biochemical characteristics test

[0016] Test method: Refer to the "Common Bacteria System Identification Manual" to conduct physiological and biochemical identification tests on lysine Bacillus fusiformis. The results are shown in Table 1.

[0017] Table 1

[0018]

[0019] Test Example 2

[0020] Collagenase and chitinase production capacity test:

[0021] Test method: Spindle-shaped lysine Bacillus was inoculated into gelatin solid culture medium and chitin solid culture medium, and inverted and cultured at 30°C for 24h and 48h respectively, and the growth state of the bacteria was observed, and the diameter of the transparent hydrolysis zone (D) and the diameter of the colony (d) were measured respectively, and the value of the diameter ratio D / d was calculated. The results are shown in Table 2.

[0022] Table 2

[0023]

[0024] From the analysis of the experimental data in Table 2, it can be seen that the spindle-shaped lysine Bacillus prepared by the present invention has excellent ability to produce collagenase and chitinase.

[0025] Test Example 3

[0026] Phosphorus and potassium dissolving performance test

[0027] Test method: Spindle-shaped lysine Bacillus was inoculated into phosphate-solubilizing solid medium and potassium-solubilizing solid medium, inverted and cultured at 30°C for 5 days and 7 days respectively, the growth state of the bacteria was observed, the diameters of the phosphate-solubilizing circle and potassium-solubilizing circle (D) and the colony diameter (d) were measured respectively, and the value of the diameter ratio D / d was calculated. The results are shown in Table 3.

[0028] Table 3

[0029]

[0030] From the experimental data analysis in Table 3, it can be seen that the spindle-shaped lysine Bacillus prepared by the present invention has excellent phosphate and potassium solubilization performance.

[0031] Test Example 4

[0032] Performance test of controlling southern root-knot nematode

[0033] Test method: The test site was the scientific research base of Shenyang Agricultural University. The diseased soil was used as the cultivation soil for tomatoes. When the tomato seedlings grew to 3 true leaves, they were transplanted into 15cm×13cm pots, with one plant transplanted per pot and 1kg of soil. Sterile water was used to adjust the concentration of the spindle-shaped Lysinibacillus fermentation liquid to 1×10 9CFU / mL, as the fermentation liquid. Take 10mL of the fermentation liquid, 10-fold dilution and 50-fold dilution respectively, and use them to irrigate the roots of tomato seedling potted plants, and do three repeated experiments. The potted plants were randomly placed in a constant temperature and light incubator at 28℃, and cultured under 16h light and 8h dark conditions. No additional fertilizer was applied, and the potted plants were watered in a timely and quantitative manner. In order to ensure a consistent environment in the constant temperature and light incubator, the position of the pots was randomly moved regularly. 45 days after transplanting, the tomato roots in the pots were poured out together with the soil, and the soil around the roots was gently patted to obtain the intact roots of the tomato plants. The soil on the root surface was gently rinsed with water to remove the soil, and the tomato plant height, stem thickness, root fresh weight, aboveground fresh weight and number of root knots were observed and recorded; at the same time, 100g of soil around the roots of the tomato plants was taken to detect the number of second-instar larvae in the soil under a microscope. The disease level was recorded according to the root-knot nematode disease level standard (Barker, KR, 1985), and the root knot index and control effect were calculated. Root knot index = Σ (number of diseased plants at each level × corresponding level value) / (total number of plants investigated × highest level) × 100%; control effect = [(control root knot index - treatment root knot index) / control root knot index] × 100%. The results are shown in Table 4.

[0034] Table 4

[0035]

[0036] From the test data analysis of Table 4, it can be seen that the fusiform Lysinibacillus prepared by the present invention has a good control effect on tomato root-knot nematodes. Different dilutions of fusiform Lysinibacillus significantly increased the stem diameter and aboveground fresh weight of tomatoes, but had no obvious effect on plant height and root fresh weight.

[0037] It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above and that the invention can be implemented in other specific forms without departing from the spirit or essential features of the invention. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations within the meaning and scope of the equivalent elements of the claims be included in the invention. Any marking in a claim should not be considered as limiting the claim to which it relates.

Claims

1. A spindle-shaped lysinophilic Bacillus ( Lysinibacillus fusiformis ), characterized in that: It was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on November 13, 2023, with the deposit number: CGMCC No.28950.

2. Use of the lysinophilic Bacillus according to claim 1 in producing collagenase.

3. Use of the lysinibacillus fusiformis according to claim 1 in producing chitinase.

4. Use of the lysinibacillus fusiformis according to claim 1 in phosphate and potassium solubilization.

Images