A composition of agarwood and moxa and a preparation method thereof and application of the composition to hangover products

Abstract

This invention provides a composition of agarwood and mugwort, its preparation method, and its application in hangover remedies, relating to the field of plant extraction technology. The hangover remedy prepared from the agarwood and mugwort composition obtained by the extraction method of this invention has excellent hangover-relieving and liver-protecting effects for patients with excessive drinking or alcohol poisoning, improving the discomfort symptoms caused by excessive drinking. The hangover remedy containing the agarwood and mugwort composition prepared by this invention accelerates the metabolism of ethanol in the liver by reducing malondialdehyde content and increasing the activity of alcohol dehydrogenase and glutathione, thus achieving the effects of relieving hangovers, protecting the liver, and alleviating hangovers. Simultaneously, the aroma of agarwood and mugwort also has a calming and soothing effect, relieving tension, anxiety, and other emotional states caused by drinking.

Description

Technical Field

[0001] This invention relates to the field of plant extraction technology, and in particular to a composition of agarwood and mugwort, its preparation method, and its application in hangover remedies. Background Technology

[0002] Long-term excessive alcohol consumption can cause alcoholic liver damage, often initially inducing fatty liver, which can then progress to alcoholic hepatitis, liver fibrosis, and cirrhosis, ultimately leading to liver dysfunction. In recent years, the harm caused by excessive drinking has attracted widespread social attention. Therefore, the research and development of hangover remedies has become a hot topic in the medical field. Currently, the treatment for alcohol poisoning caused by drinking often involves intravenous injection of naloxone, etc. Developing hangover remedies from traditional Chinese medicine has enormous potential. my country is rich in traditional Chinese medicinal resources, and hangover remedies made from traditional Chinese medicine are booming. Traditional Chinese medicine preparations are widely favored by people due to their fewer side effects, unique efficacy, and low price.

[0003] Agarwood is the woody part containing black resin from the Aquilaria sinensis or Aquilaria agallocha plants of the Thymelaeaceae family. Modern pharmacological studies have shown that agarwood has sedative, antiarrhythmic, central nervous system inhibitory, antibacterial, and anti-inflammatory effects. Artemisia floss is the downy part of the leaves of Artemisia argyi, a traditional Chinese medicine. Artemisia argyi is a common Chinese herbal medicine with warming, dispersing, blood-activating, and insect-expelling effects. Artemisia floss is often used in moxibustion and decoctions of traditional Chinese medicine, providing auxiliary therapeutic effects. It can also be used in some external ointments and plasters. In traditional Chinese medicine health preservation, some people choose to use artemisia floss for moxibustion. However, there is currently little research on the use of a combination of agarwood and artemisia floss in hangover remedies.

[0004] Therefore, the purpose of this invention is to develop a composition of agarwood and mugwort for preparing hangover remedies, which has the effect of clearing the liver and relieving hangovers, and improving the discomfort symptoms caused by excessive drinking. Summary of the Invention

[0005] In view of this, the present invention proposes a composition of agarwood and mugwort, its preparation method and its application in hangover remedies.

[0006] The technical solution of this invention is implemented as follows:

[0007] A composition of agarwood and mugwort floss, prepared from agarwood and mugwort floss in a mass ratio of 3-5:1.

[0008] A method for preparing a composition of agarwood and mugwort floss includes the following steps: pulverizing and sieving agarwood and mugwort floss, adding water, and ultrasonically extracting at 20-30℃ for 30-50 min; then raising the temperature to 60-80℃ and the pressure to 0.6-0.8 MPa for 1-3 h; finally adjusting the temperature to 40-50℃ and reducing the pressure to 0.08-0.1 MPa to obtain the extract, and concentrating under reduced pressure to obtain the composition of agarwood and mugwort floss.

[0009] Furthermore, the agarwood and mugwort floss are pulverized through a 30-50 mesh sieve.

[0010] Furthermore, the total mass of the agarwood and mugwort floss is in a liquid-to-water ratio of 1:25-30 g / mL.

[0011] Application of a combination of agarwood and mugwort in the preparation of hangover remedies.

[0012] A hangover remedy comprising the aforementioned agarwood and mugwort composition.

[0013] Furthermore, the concentration of the agarwood and mugwort composition in the hangover remedy is 7.7-14.2 wt%.

[0014] Furthermore, the agarwood and mugwort composition is prepared by the aforementioned preparation method.

[0015] Furthermore, the hangover remedy is a decoction or tablets.

[0016] A method for preparing a hangover remedy includes the following steps: mixing a composition of agarwood and mugwort, sodium carboxymethyl cellulose, hydroxypropyl methylcellulose and polyvinylpyrrolidone evenly, wherein the mass ratio of the agarwood and mugwort composition to sodium carboxymethyl cellulose, hydroxypropyl methylcellulose and polyvinylpyrrolidone is 1:3-5:1-3:2-4; granulating the mixture in a granulator; and compressing the mixture into tablets to obtain a hangover remedy tablet.

[0017] Compared with existing technologies, the beneficial effects of this invention are as follows: The hangover remedy prepared from the agarwood and mugwort composition of this invention has the effects of clearing and protecting the liver and relieving hangovers, improving the discomfort symptoms caused by excessive drinking. Agarwood has the effects of soothing the mind and calming the nerves, regulating qi and relieving pain, and moistening the lungs and resolving phlegm. Mugwort has the effects of warming the meridians and dispelling cold, and promoting blood circulation and relieving pain. This invention efficiently extracts the effective components of agarwood and mugwort, combines them synergistically, promotes blood circulation, accelerates metabolism, speeds up the onset of drug effects, improves the imbalance state inside the body, and can also relieve symptoms such as systemic pain caused by excessive drinking, without side effects. The aroma of agarwood and mugwort also has a calming and soothing effect, which can relieve tension, anxiety and other emotional states caused by drinking. The hangover remedy prepared from the agarwood and mugwort composition of this invention can significantly reduce MDA content and increase ADH and GSH activity, thereby accelerating the metabolism of ethanol in the liver, achieving the effects of relieving hangovers, protecting the liver, and relieving hangovers. Detailed Implementation

[0018] To better understand the technical content of this invention, specific embodiments are provided below to further illustrate the invention.

[0019] Unless otherwise specified, the experimental methods used in the embodiments of this invention are all conventional methods.

[0020] Unless otherwise specified, all materials and reagents used in the embodiments of this invention are commercially available.

[0021] Example 1

[0022] S1. Grind agarwood and mugwort in a mass ratio of 4:1, mix the powder and pass it through a 40-mesh sieve. Add distilled water with a total mass ratio of 1:28 g / mL to agarwood and mugwort, and perform ultrasonic extraction at 25℃ for 40 min to obtain the extract.

[0023] S2. At a temperature of 70℃ and a pressure of 0.7 MPa, the extract of S1 was extracted for another 2 hours. Finally, the temperature was lowered to 45℃ and the pressure was lowered to 0.09 MPa to obtain the extract. The extract was then concentrated under reduced pressure to obtain the agarwood and mugwort composition.

[0024] S3. Mix the agarwood and mugwort composition, sodium carboxymethyl cellulose, hydroxypropyl methylcellulose and polyvinylpyrrolidone in S2 evenly, wherein the mass ratio of the agarwood and mugwort composition to sodium carboxymethyl cellulose, hydroxypropyl methylcellulose and polyvinylpyrrolidone is 1:4:2:3. Put the mixture into a granulator to granulate, compress into tablets, and obtain a tablet hangover relief product, wherein the concentration of the agarwood and mugwort composition in the tablet hangover relief product is 10wt%.

[0025] Example 2

[0026] S1. Grind agarwood and mugwort in a mass ratio of 3:1, mix the powder and pass it through a 30-mesh sieve. Add distilled water with a total mass ratio of 1:25 g / mL to agarwood and mugwort, and perform ultrasonic extraction at a temperature of 20℃ for 30 minutes to obtain the extract.

[0027] S2. At a temperature of 60℃ and a pressure of 0.6 MPa, the extract of S1 was extracted for another 1 hour. Finally, the temperature was lowered to 40℃ and the pressure was lowered to 0.08 MPa to obtain the extract. The extract was then concentrated under reduced pressure to obtain the agarwood and mugwort composition.

[0028] S3. Mix the agarwood and mugwort composition, sodium carboxymethyl cellulose, hydroxypropyl methylcellulose and polyvinylpyrrolidone from S2 evenly, wherein the mass ratio of the agarwood and mugwort composition to sodium carboxymethyl cellulose, hydroxypropyl methylcellulose and polyvinylpyrrolidone is 1:3:1:2. Granulate the mixture in a granulator and compress it into tablets to obtain a hangover relief product, wherein the concentration of the agarwood and mugwort composition in the hangover relief product is 14.2 wt%.

[0029] Example 3

[0030] S1. Grind agarwood and mugwort in a mass ratio of 5:1, mix the powder and pass it through a 50-mesh sieve. Add distilled water with a total mass ratio of 1:30 g / mL to agarwood and mugwort, and perform ultrasonic extraction at a temperature of 30℃ for 50 min to obtain the extract.

[0031] S2. At a temperature of 80℃ and a pressure of 0.8 MPa, the extract of S1 was extracted for another 3 hours. Finally, the temperature was reduced to 50℃ and the pressure was reduced to 0.1 MPa to obtain the extract. The extract was then concentrated under reduced pressure to obtain the agarwood and mugwort composition.

[0032] S3. Mix the agarwood and mugwort composition, sodium carboxymethyl cellulose, hydroxypropyl methylcellulose and polyvinylpyrrolidone in S2 evenly, wherein the mass ratio of the agarwood and mugwort composition to sodium carboxymethyl cellulose, hydroxypropyl methylcellulose and polyvinylpyrrolidone is 1:5:3:4. Granulate the mixture in a granulator and compress it into tablets to obtain a hangover relief product, wherein the concentration of the agarwood and mugwort composition in the hangover relief product is 7.7 wt%.

[0033] Example 4

[0034] S1. Grind agarwood and mugwort in a mass ratio of 4:1, mix the powder and pass it through a 40-mesh sieve. Add distilled water with a total mass ratio of 1:28 g / mL to agarwood and mugwort, and perform ultrasonic extraction at 25℃ for 40 min to obtain the extract.

[0035] S2. At a temperature of 70℃ and a pressure of 0.7 MPa, the extract of S1 was extracted for another 2 hours. Finally, the temperature was lowered to 45℃ and the pressure was lowered to 0.09 MPa to obtain the extract. The extract was then concentrated under reduced pressure to obtain the agarwood and mugwort composition.

[0036] S3. Dissolve the agarwood and mugwort composition, honey and rock sugar in water and stir evenly. The mass ratio of the agarwood and mugwort composition to the honey and rock sugar is 1:5:4. Simmer over low heat to obtain a decoction for relieving hangovers. The concentration of the agarwood and mugwort composition in the decoction for relieving hangovers is 10wt%.

[0037] Comparative Example 1

[0038] The difference from Example 1 is that it lacks agarwood extract; otherwise, it is the same as Example 1.

[0039] S1. Crush the mugwort floss, pass the powder through a 40-mesh sieve, add distilled water at a ratio of 1:28 g / mL to the mugwort floss, and perform ultrasonic extraction for 40 minutes at a temperature of 25℃ to obtain the extract.

[0040] S2. At a temperature of 70℃ and a pressure of 0.7 MPa, the extract from S1 was extracted for another 2 hours. Finally, the temperature was lowered to 45℃ and the pressure was lowered to 0.09 MPa to obtain the extract. The extract was then concentrated under reduced pressure to obtain the Artemisia argyi extract.

[0041] S3. Mix the Artemisia argyi extract, sodium carboxymethyl cellulose, hydroxypropyl methylcellulose and polyvinylpyrrolidone from S2 evenly, wherein the mass ratio of Artemisia argyi extract to sodium carboxymethyl cellulose, hydroxypropyl methylcellulose and polyvinylpyrrolidone is 1:4:2:3. Put the mixture into a granulator to granulate, compress into tablets, and obtain a tablet product for relieving hangovers, wherein the concentration of Artemisia argyi extract in the tablet product for relieving hangovers is 10wt%.

[0042] Comparative Example 2

[0043] The difference from Example 1 is that the Artemisia argyi extract is missing; otherwise, it is the same as Example 1.

[0044] S1. Crush the agarwood, pass the powder through a 40-mesh sieve, add distilled water with a material-to-liquid ratio of 1:28 g / mL to the agarwood, and perform ultrasonic extraction for 40 minutes at a temperature of 25℃ to obtain the extract.

[0045] S2. At a temperature of 70℃ and a pressure of 0.7 MPa, the extract from S1 was extracted for another 2 hours. Finally, the temperature was lowered to 45℃ and the pressure was lowered to 0.09 MPa to obtain the extract. The extract was then concentrated under reduced pressure to obtain the agarwood extract.

[0046] S3. Mix the agarwood extract, sodium carboxymethyl cellulose, hydroxypropyl methylcellulose and polyvinylpyrrolidone from S2 evenly, wherein the mass ratio of agarwood extract to sodium carboxymethyl cellulose, hydroxypropyl methylcellulose and polyvinylpyrrolidone is 1:4:2:3. Put the mixture into a granulator to granulate, compress into tablets, and obtain a hangover relief tablet product, wherein the concentration of Artemisia argyi extract in the hangover relief tablet product is 10wt%.

[0047] Comparative Example 3

[0048] The difference from Example 1 is that the mass ratio of agarwood to mugwort is 1:1; otherwise, it is the same as Example 1.

[0049] S1. Grind agarwood and mugwort in a mass ratio of 1:1, mix the powders and pass them through a 40-mesh sieve. Add distilled water in a mass ratio of 1:28 g / mL to the total mass of agarwood and mugwort, and perform ultrasonic extraction at 25℃ for 40 min to obtain the extract.

[0050] S2. At a temperature of 70℃ and a pressure of 0.7 MPa, the extract of S1 was extracted for another 2 hours. Finally, the temperature was lowered to 45℃ and the pressure was lowered to 0.09 MPa to obtain the extract. The extract was then concentrated under reduced pressure to obtain the agarwood and mugwort composition.

[0051] S3. Mix the agarwood and mugwort composition, sodium carboxymethyl cellulose, hydroxypropyl methylcellulose and polyvinylpyrrolidone in S2 evenly, wherein the mass ratio of the agarwood and mugwort composition to sodium carboxymethyl cellulose, hydroxypropyl methylcellulose and polyvinylpyrrolidone is 1:4:2:3. Put the mixture into a granulator to granulate, compress into tablets, and obtain a tablet hangover relief product, wherein the concentration of the agarwood and mugwort composition in the tablet hangover relief product is 10wt%.

[0052] Comparative Example 4

[0053] S1. Grind agarwood and mugwort in a mass ratio of 4:1, mix the powder and pass it through a 40-mesh sieve. Add distilled water with a total mass ratio of 1:28 g / mL to agarwood and mugwort, extract at 25℃ for 40 min, filter to obtain the aqueous extract, and collect the filter residue.

[0054] S2. Add 10 times the weight of 80% v / v ethanol solution to the filter residue and extract for 60 min. Reflux with ultrasound at 70℃. Collect the ethanol extract and combine it with the water extract from S1. Concentrate under reduced pressure to obtain the agarwood and mugwort composition.

[0055] S3. Mix the agarwood and mugwort composition, sodium carboxymethyl cellulose, hydroxypropyl methylcellulose and polyvinylpyrrolidone in S2 evenly, wherein the mass ratio of the agarwood and mugwort composition to sodium carboxymethyl cellulose, hydroxypropyl methylcellulose and polyvinylpyrrolidone is 1:4:2:3. Put the mixture into a granulator to granulate, compress into tablets, and obtain a tablet hangover relief product, wherein the concentration of the agarwood and mugwort composition in the tablet hangover relief product is 10wt%.

[0056] Test case

[0057] 1. Modeling Methods

[0058] One hundred clean-grade male rats (weighing 200±10g) were purchased and randomly divided into a blank control group (n=10) and a model group (n=90). The model group was administered 50% alcohol by gavage at a dose of 20mL / kg of body weight for 20 consecutive weeks to establish an animal model of alcohol poisoning caused by excessive drinking. The model was successfully established in 90 rats.

[0059] 2. Administration method

[0060] The administration method was gavage. Ninety rats with alcohol poisoning due to excessive drinking were randomly divided into nine groups: the model group, Examples 1-4, and Comparative Examples 1-4, with 10 rats in each group. The dosage was 0.5g twice daily for two weeks. The blank control group and the model group were given the same volume of physiological saline.

[0061] 3. Detection indicators

[0062] Alcohol dehydrogenase is an enzyme involved in ethanol metabolism, primarily responsible for converting ethanol into acetaldehyde. In the liver, the activity of alcohol dehydrogenase reflects the status of ethanol metabolism and is significant for the diagnosis and monitoring of alcohol consumption. Due to the free radicals and oxidative stress generated by alcohol metabolism, liver cells suffer oxidative damage, and malondialdehyde, a product of lipid peroxidation, will increase in its concentration. Glutathione plays a role in scavenging free radicals and protecting cells from oxidative damage within cells.

[0063] After administration, rats were anesthetized by intraperitoneal injection of sodium pentobarbital. Blood was collected from the abdominal main vein to determine biochemical indicators. The liver was removed and homogenized into a 10% liver homogenate with physiological saline and centrifuged. The hangover-relieving effect of the product of this invention was evaluated by measuring the contents of alcohol dehydrogenase, malondialdehyde (GSH), and glutathione. ADH is alcohol dehydrogenase, GSH is glutathione, and MDA is malondialdehyde. Specific test data are shown in Table 1.

[0064] Table 1

[0065] Group ADH (U / mL) GSH (mg / g) MDA (nmol / mg) Blank control group 3.16±0.33 15.65±2.78 2.08±0.27 Model group <![CDATA[1.06±0.53 α ]]> <![CDATA[10.23±2.44 α ]]> <![CDATA[3.84±0.35 α ]]> Example 1 <![CDATA[3.04±0.28 β ]]> <![CDATA[15.71±2.79 β ]]> <![CDATA[2.09±0.11 β ]]> Example 2 <![CDATA[3.01±0.26 β ]]> <![CDATA[15.51±2.34 β ]]> <![CDATA[2.11±0.12 β ]]> Example 3 <![CDATA[2.99±0.27 β ]]> <![CDATA[15.49±2.55 β ]]> <![CDATA[2.12±0.14 β ]]> Example 4 <![CDATA[3.05±0.24 β ]]> <![CDATA[15.69±2.75 β ]]> <![CDATA[2.09±0.13 β <!-- 4 -->]]> Comparative Example 1 <![CDATA[1.26±0.38 β ]]> <![CDATA[10.65±2.33 β ]]> <![CDATA[3.65±0.24 β ]]> Comparative Example 2 <![CDATA[1.28±0.31 β ]]> <![CDATA[10.84±2.18 β ]]> <![CDATA[3.63±0.21 β ]]> Comparative Example 3 <![CDATA[1.45±0.33 β ]]> <![CDATA[13.84±2.21 β ]]> <![CDATA[3.23±0.24 β ]]> Comparative Example 4 <![CDATA[1.38±0.32 β ]]> <![CDATA[12.84±2.14 β ]]> <![CDATA[3.31±0.30 β ]]>

[0066] Note: α Compared with the blank control group, P<0.05; β Compared with the model group, P<0.05

[0067] As shown in Table 1, after 2 weeks, compared with the blank control group, the model group showed a significant decrease in ADH and GSH, and a significant increase in MDA. Compared with the model group, Examples 1-4 significantly reduced MDA (P<0.05) and increased ADH and GSH (P<0.05). This indicates that the hangover remedy prepared by this invention, containing agarwood and artemisia flakes, can significantly reduce MDA content and increase ADH and GSH activity, thereby accelerating the metabolism of ethanol in the liver and achieving the effects of relieving hangovers, protecting the liver, and sobering up.

[0068] Comparative Example 1 differs from Example 1 in that it lacks agarwood extract; Comparative Example 2 differs from Example 1 in that it lacks mugwort extract; and Comparative Example 3 differs from Example 1 in that the mass ratio of agarwood to mugwort is 1:1. Table 1 shows that, compared to Example 1, the ADH and GSH activities (P<0.05) of Comparative Examples 1-3 are worse, while the MDA content is higher, resulting in a poorer sobering effect. This indicates that the agarwood and mugwort composition of the present invention is scientifically formulated and synergistically enhances the effect, making the sobering product of the present invention more effective.

[0069] The difference between Comparative Example 4 and Example 1 lies in the extraction method of the agarwood and mugwort composition: conventional water extraction followed by alcohol extraction. Table 1 shows that the ADH and GSH activities (P<0.05) of Comparative Example 4 decreased, while the MDA content (P<0.05) increased, resulting in a poorer sobering effect. This indicates that the sobering product prepared from the agarwood and mugwort composition obtained using the extraction method of this invention has excellent sobering and liver-protecting effects for patients with excessive drinking or alcohol poisoning.

[0070] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. The application of a composition of agarwood and mugwort in the preparation of hangover remedies, characterized in that, The agarwood and mugwort composition is prepared from agarwood and mugwort in a mass ratio of 3-5:1; The specific steps of the preparation method of the agarwood and mugwort composition include: pulverizing and sieving the agarwood and mugwort, adding water, and ultrasonically extracting at 20-30℃ for 30-50 minutes. Then, raising the temperature to 60-80℃ and the pressure to 0.6-0.8 MPa, extracting for 1-3 hours. Finally, adjusting the temperature to 40-50℃ and reducing the pressure to 0.08-0.1 MPa to obtain the extract, and concentrating under reduced pressure to obtain the agarwood and mugwort composition.

2. The application as described in claim 1, characterized in that, In the preparation method of the agarwood and mugwort composition, the agarwood and mugwort are pulverized and passed through a 30-50 mesh sieve.

3. The application as described in claim 1, characterized in that, In the preparation method of the agarwood and mugwort composition, the total mass of the agarwood and mugwort is in a material-to-liquid ratio of 1:25-30 g / mL to water.

4. A hangover remedy, characterized in that, It comprises the agarwood and mugwort composition as described in claim 1.

5. The hangover remedy as described in claim 4, characterized in that, The concentration of the agarwood and mugwort composition in the hangover remedy is 7.7-14.2 wt%.

6. The hangover remedy as described in claim 4, characterized in that, The hangover remedy mentioned is either a decoction or tablets.

7. The hangover remedy according to claim 6, characterized in that, The specific steps of the preparation method of the tablet hangover remedy product include: mixing agarwood and mugwort composition, sodium carboxymethyl cellulose, hydroxypropyl methylcellulose and polyvinylpyrrolidone evenly, wherein the mass ratio of the agarwood and mugwort composition to sodium carboxymethyl cellulose, hydroxypropyl methylcellulose and polyvinylpyrrolidone is 1:3-5:1-3:2-4, granulating the mixture in a granulator, and compressing it into tablets to obtain the tablet hangover remedy product.