A method for breeding a high alkali consumption value three-line sterile line of rice in Guangxi Zhuang Autonomous Region

Abstract

The present application relates to rice breeding technology field, and provides a high alkali consumption value Guangxi Zhuang autonomous region rice three-line sterile line breeding method, which comprises the following steps: taking a high alkali consumption value variety in Guangxi Zhuang autonomous region as a male parent, taking a three-line maintainer line rice variety as a female parent, hybridizing to obtain F1 generation; backcrossing the female parent with F1 generation to obtain BC1F1 generation; continuously backcrossing to obtain BC5F1 generation, selfing to obtain BC5F2 generation; continuously selfing BC5F2 generation to obtain a three-line maintainer line; hybridizing the three-line maintainer line as a male parent with a three-line sterile line rice variety to obtain a sterile line rice; backcrossing the sterile line rice with the three-line maintainer line to obtain backcross progeny; taking the three-line maintainer line as a recurrent male parent, selecting a target plant in the backcross progeny for continuous backcrossing to obtain a high alkali consumption value rice three-line sterile line. The method is simple and easy to operate, and provides an excellent rice three-line sterile line. It can be used for rice improvement and quality improvement of rice.

Description

TECHNICAL FIELD

[0001] The application relates to the technical field of rice breeding, and in particular to a high-alkali-elimination-value Guangxi Zhuang Autonomous Region rice three-line sterile line breeding method. BACKGROUND

[0002] The alkali elimination value refers to the erosion degree of alkali liquor to milled rice grains, and the alkali elimination value is an effective method for evaluating the gelatinization temperature of rice. In actual rice quality evaluation, the gelatinization temperature is often indirectly measured by measuring the alkali elimination value, and the method is simple to operate. The alkali elimination value is opposite to the gelatinization temperature, that is, the greater the alkali elimination value, the lower the corresponding gelatinization temperature, and vice versa.

[0003] According to the provisions in the industry standard, the alkali elimination value of 1, 2 high-quality indica rice should be greater than or equal to 6.0, and the alkali elimination value of 3 high-quality indica rice should be greater than or equal to 5.0; the alkali elimination value of 1, 2 high-quality japonica rice should be greater than or equal to 7.0, and the alkali elimination value of 3 high-quality japonica rice should be greater than or equal to 6.0.

[0004] However, the alkali elimination value of hybrid rice is currently low, generally about 4.5-6.0, which seriously affects the cooking quality of rice and the standard rate of high-quality rice. Therefore, breeding rice varieties with high alkali elimination value is very important for the development of hybrid rice. SUMMARY

[0005] The purpose of the application is to provide a high-alkali-elimination-value Guangxi Zhuang Autonomous Region rice three-line sterile line breeding method, and to provide an excellent rice three-line sterile line.

[0006] In order to achieve the above-mentioned application purposes, the application provides the following technical solutions:

[0007] The application provides a high-alkali-elimination-value Guangxi Zhuang Autonomous Region rice three-line sterile line breeding method, which comprises the following steps:

[0008] (1) a Guangxi Zhuang Autonomous Region high-alkali-elimination-value variety is used as a male parent, a three-line maintainer rice variety is used as a female parent, and F1 generation is obtained by hybridization;

[0009] (2) the three-line maintainer rice variety is used as the female parent and backcrossed with the F1 generation to obtain the BC1F1 generation; a target plant is selected, and continuous backcrossing is performed for 5 times to obtain the BC5F1 generation;

[0010] (3) the target plant is selected from the BC5F1 generation and selfed to obtain the BC5F2 generation;

[0011] (4) the target plant with the characteristics of the maintainer line and the high-alkali-elimination-value is selected from the BC5F2 generation and continuously selfed to obtain the high-alkali-elimination-value rice three-line maintainer line;

[0012] (5) take the high alkali elimination value rice three-line maintainer line as the father, the three-line sterile line rice variety in step (1) as the mother, and select the sterile line rice after hybridization;

[0013] (6) backcross the sterile line rice in step (5) with the high alkali elimination value rice three-line maintainer line to obtain the backcross progeny;

[0014] (7) take the high alkali elimination value rice three-line maintainer line as the recurrent father, and select the target plant in the backcross progeny for continuous backcrossing to obtain the high alkali elimination value rice three-line sterile line.

[0015] As preferred, the high alkali elimination value variety in Guangxi Zhuang Autonomous Region in step (1) is Gui 3158 or Gui 582, and the three-line maintainer line rice variety is Keyuan B.

[0016] As preferred, the selection criteria of the target plant in steps (2)-(4) and (7) are: alkali elimination value 6.5-7.0, moderate plant height, thick and strong stem, and good color change at the mature stage.

[0017] As preferred, the number of generations of continuous self-crossing in step (4) is 5-7 generations.

[0018] As preferred, the number of generations of continuous backcrossing in step (7) is 5-6 generations.

[0019] The application provides a high alkali elimination value rice three-line sterile line breeding method in Guangxi Zhuang Autonomous Region, which comprises the following steps: taking a high alkali elimination value variety in Guangxi Zhuang Autonomous Region as the father, taking a three-line maintainer line rice variety as the mother, and hybridizing to obtain F1 generation; backcrossing the mother with F1 generation to obtain BC1F1 generation; continuously backcrossing to obtain BC5F1 generation, self-crossing to obtain BC5F2 generation; continuously self-crossing BC5F2 generation to obtain a three-line maintainer line; hybridizing the three-line maintainer line as the father with a three-line sterile line rice variety to obtain a sterile line rice; backcrossing the sterile line rice with the three-line maintainer line to obtain backcross progeny; taking the three-line maintainer line as the recurrent father, and selecting a target plant in the backcross progeny for continuous backcrossing to obtain a high alkali elimination value rice three-line sterile line. The method is simple and easy to operate, and provides an excellent rice three-line sterile line. The method can be used for rice improvement and quality improvement of rice. DETAILED DESCRIPTION

[0020] The application provides a high alkali elimination value rice three-line sterile line breeding method in Guangxi Zhuang Autonomous Region, which comprises the following steps:

[0021] (1) taking a high alkali elimination value variety in Guangxi Zhuang Autonomous Region as the father, and taking a three-line maintainer line rice variety as the mother, and hybridizing to obtain F1 generation;

[0022] (2) Using a three-line maintainer rice variety as the female parent, backcross with the F1 generation to obtain the BC1F1 generation; select target plants and backcross 5 times consecutively to obtain the BC5F1 generation;

[0023] (3) Select target plants from the BC5F1 generation for self-pollination to obtain the BC5F2 generation;

[0024] (4) Select target plants with maintainer line and high alkali reduction value characteristics from BC5F2 generation and conduct continuous self-pollination to obtain a three-line maintainer line of high alkali reduction value rice.

[0025] (5) The high alkali reduction value rice three-line maintainer line is used as the male parent and the three-line sterile rice variety in step (1) is used as the female parent. After hybridization, sterile rice is selected.

[0026] (6) Backcross the sterile rice line from step (5) with the three-line maintainer line of high alkali reduction rice to obtain backcross offspring;

[0027] (7) Using the high alkali reduction value rice three-line maintainer line as the recurrent male parent, target plants were selected from the backcross progeny for continuous backcrossing to obtain the high alkali reduction value rice three-line sterile line.

[0028] In this invention, the high alkali reduction value variety of Guangxi Zhuang Autonomous Region mentioned in step (1) is preferably Gui 3158 or Gui 582, and the three-line maintainer rice variety is preferably Keyuan B.

[0029] In this invention, the selection criteria for the target plants in steps (2) to (4) and (7) are: alkali spreading value of 6.5 to 7.0, moderate plant height, thick stem, and good color change at maturity.

[0030] In this invention, the number of generations of continuous self-crossing in step (4) is preferably 5 to 7, and more preferably 6.

[0031] In this invention, the number of generations of continuous backcrossing in step (7) is preferably 5 to 6, and more preferably 5.

[0032] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0033] Example 1

[0034] A method for breeding three-line male-sterile rice with high alkali digestibility in Guangxi Zhuang Autonomous Region includes the following steps:

[0035] (1) Using the high alkali reduction value variety Gui 3158 from Guangxi Zhuang Autonomous Region as the male parent and the three-line maintainer rice variety Keyuan B as the female parent, the F1 generation was obtained by hybridization;

[0036] (2) take the three-line maintainer rice variety as the female parent and backcross with the F1 generation to obtain the BC1F1 generation; select target plants and continuously backcross 5 times to obtain the BC5F1 generation;

[0037] (3) select target plants from the BC5F1 generation for selfing to obtain the BC5F2 generation;

[0038] (4) select target plants with the characteristics of the maintainer line and high alkali solubility from the BC5F2 generation for continuous selfing for 5 generations to obtain a high-alkali-solubility rice three-line maintainer line;

[0039] (5) take the high-alkali-solubility rice three-line maintainer line as the male parent and the three-line sterile rice variety in step (1) as the female parent, and select a sterile rice line after hybridization;

[0040] (6) backcross the sterile rice line in step (5) with the high-alkali-solubility rice three-line maintainer line to obtain a backcross progeny;

[0041] (7) take the high-alkali-solubility rice three-line maintainer line as a recurrent male parent, and select target plants from the backcross progeny for continuous backcrossing for 6 generations to obtain a high-alkali-solubility rice three-line sterile line.

[0042] Note: The selection criteria for the target plants in steps (2)-(4) and (7) are: alkali solubility of 6.5-7.0, moderate plant height, thick and strong stem, and good color change at the mature stage.

[0043] Example 2

[0044] A method for breeding a high-alkali-solubility rice three-line sterile line in Guangxi Zhuang Autonomous Region, comprising the following steps:

[0045] (1) take a high-alkali-solubility variety Gui582 in Guangxi Zhuang Autonomous Region as the male parent and a three-line maintainer rice variety Keyuan B rice as the female parent, and hybridize to obtain an F1 generation;

[0046] (2) take the three-line maintainer rice variety as the female parent and backcross with the F1 generation to obtain a BC1F1 generation; select target plants and continuously backcross 5 times to obtain a BC5F1 generation;

[0047] (3) select target plants from the BC5F1 generation for selfing to obtain a BC5F2 generation;

[0048] (4) select target plants with the characteristics of the maintainer line and high alkali solubility from the BC5F2 generation for continuous selfing for 7 generations to obtain a high-alkali-solubility rice three-line maintainer line;

[0049] (5) take the high-alkali-solubility rice three-line maintainer line as the male parent and the three-line sterile rice variety in step (1) as the female parent, and select a sterile rice line after hybridization;

[0050] (6) the sterile line rice of step (5) is crossed with the high alkali value rice three-line maintainer line to obtain a backcross progeny;

[0051] (7) the high alkali value rice three-line maintainer line is used as a recurrent male parent, and a target plant is selected from the backcross progeny for continuous backcrossing for 5 generations to obtain a high alkali value rice three-line sterile line.

[0052] Note: the selection criteria for the target plant in steps (2)-(4) and (7) are: alkali value 6.5-7.0, moderate plant height, thick stem, and good color change at the mature stage.

[0053] Example 3

[0054] A method for breeding a high alkali value rice three-line sterile line in Guangxi Zhuang Autonomous Region, comprising the following steps:

[0055] (1) a high alkali value variety Gui 3158 in Guangxi Zhuang Autonomous Region is used as a male parent, and a three-line maintainer line rice variety Keyuan B is used as a female parent to obtain an F1 generation;

[0056] (2) the three-line maintainer line rice variety is used as a female parent to cross with the F1 generation to obtain a BC1F1 generation; a target plant is selected, and continuous backcrossing is performed for 5 times to obtain a BC5F1 generation;

[0057] (3) the target plant is selected from the BC5F1 generation for selfing to obtain a BC5F2 generation;

[0058] (4) the target plant with the characteristics of the maintainer line and the high alkali value is selected from the BC5F2 generation for continuous selfing for 6 generations to obtain a high alkali value rice three-line maintainer line;

[0059] (5) the high alkali value rice three-line maintainer line is used as a male parent, and the three-line sterile line rice variety in step (1) is used as a female parent, and a sterile line rice is selected after crossing;

[0060] (6) the sterile line rice of step (5) is crossed with the high alkali value rice three-line maintainer line to obtain a backcross progeny;

[0061] (7) the high alkali value rice three-line maintainer line is used as a recurrent male parent, and a target plant is selected from the backcross progeny for continuous backcrossing for 6 generations to obtain a high alkali value rice three-line sterile line.

[0062] Note: the selection criteria for the target plant in steps (2)-(4) and (7) are: alkali value 6.5-7.0, moderate plant height, thick stem, and good color change at the mature stage.

[0063] Test Example

[0064] The three-line sterile rice and the three-line maintaining rice prepared in the examples 1-3 are taken, the sterile plant rate and the sterility degree of each group of sterile rice are identified, and the alkali digestion value of the maintaining rice is detected, and the results are shown in Table 1.

[0065] Table 1

[0066]

[0067] From the above examples, it can be seen that the application provides a high-alkali-digestion-value Guangxi Zhuang Autonomous Region rice three-line sterile line breeding method, which comprises the following steps: taking a high-alkali-digestion-value variety in the Guangxi Zhuang Autonomous Region as a male parent, taking a three-line maintaining rice variety as a female parent, and hybridizing to obtain an F1 generation; backcrossing the female parent with the F1 generation to obtain a BC1F1 generation; continuously backcrossing to obtain a BC5F1 generation, selfing to obtain a BC5F2 generation; continuously selfing the BC5F2 generation to obtain a three-line maintaining line; hybridizing the three-line maintaining line as a male parent with a three-line sterile rice variety to obtain a sterile line rice; backcrossing the sterile line rice with the three-line maintaining line to obtain a backcross progeny; and taking the three-line maintaining line as a recurrent male parent, selecting a target plant in the backcross progeny for continuous backcrossing to obtain a high-alkali-digestion-value rice three-line sterile line. The method of the application is simple and easy to operate, and provides an excellent rice three-line sterile line. The method can be used for rice improvement and quality improvement of rice.

[0068] The above only describes the preferred embodiments of the application, and it should be noted that, for those skilled in the art, without departing from the principles of the application, several improvements and refinements can be made, and these improvements and refinements should also be considered as the protection scope of the application.

Claims

1. A method for breeding high-alkali-digestion-value three-line male-sterile rice in Guangxi Zhuang Autonomous Region, characterized in that, Includes the following steps: (1) Using a high alkali reduction value variety from Guangxi Zhuang Autonomous Region as the male parent and a three-line maintainer rice variety as the female parent, the F1 generation was obtained by hybridization; (2) Using a three-line maintainer rice variety as the female parent and backcrossing it with the F1 generation, the BC1F1 generation was obtained; Select target plants and backcross them five times consecutively to obtain the BC5F1 generation. (3) Select target plants from the BC5F1 generation for self-pollination to obtain the BC5F2 generation; (4) Select target plants with maintainer line and high alkali reduction value characteristics from BC5F2 generation and conduct continuous self-pollination to obtain a three-line maintainer line of high alkali reduction value rice; (5) Using the high alkali reduction value rice three-line maintainer line as the male parent and the three-line male sterile rice variety as the female parent, the male sterile rice was obtained by hybridization; (6) Backcross the sterile rice line from step (5) with the three-line maintainer line of high alkali reduction rice to obtain backcross offspring; (7) Using the high alkali reduction value rice three-line maintainer line as the recurrent male parent, target plants were selected from the backcross progeny for continuous backcrossing to obtain the high alkali reduction value rice three-line sterile line; The high alkali reduction value rice varieties mentioned in step (1) of Guangxi Zhuang Autonomous Region are Gui 3158 or Gui 582, and the three-line maintainer rice variety is Keyuan B; The selection criteria for the target plants mentioned in steps (2) to (4) and (7) are: alkali spreading value of 6.5 to 7.0, moderate plant height, thick stem, and good color change at maturity.

2. The breeding method according to claim 1, characterized in that, The number of generations of continuous self-crossing mentioned in step (4) is 5 to 7.

3. The breeding method according to any one of claims 1 to 2, characterized in that, The number of generations of continuous backcrossing mentioned in step (7) is 5 to 6.

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