A method for breeding hemp-feathered broilers with high inosinic acid content

Abstract

The invention discloses a breeding method for hemp-feather broilers with high inosinic acid content, which relates to the technical field of poultry breeding, comprising the following steps: obtaining an F1 generation hemp-feather broiler female parent by hybridizing a Hetian chicken male parent and a Chongren hemp chicken female parent, obtaining an F2 generation hemp-feather broiler female parent after backcrossing with the Hetian chicken male parent, hybridizing the F2 generation hemp-feather broiler female parent and a Qingyuan hemp chicken male parent to obtain an F3 generation hemp-feather broiler, cross-crossing and fixing the F3 generation hemp-feather broiler to obtain an hemp-feather broiler with high inosinic acid content; the invention The Hetian chicken male and the Chongren Ma chicken female were selected and hybridized to obtain the F1 generation of Ma Yu broilers. The F1 generation of Ma Yu broilers female was then backcrossed with the Hetian chicken male to obtain the F2 generation of Ma Yu broilers. The F2 generation of Ma Yu broilers were used as the female parent and the Qingyuan Ma chicken was selected as the male parent for hybridization to obtain the F3 generation of Ma Yu broilers. After that, male and female individuals with growth rates that met the standards were selected from the F3 generation of Ma Yu broilers for cross-crossing and fixation to obtain Ma Yu broilers with high inosinic acid content.

Description

Technical Field

[0001] The invention relates to the technical field of poultry breeding, and in particular to a breeding method for hemp-feather broilers with high inosinic acid content. Background Art

[0002] Inosinic acid (inosincacid, inosinemonphosphate, IMP), also known as inosinic acid or inosinic acid, is a nucleotide found in ribonucleic acid (RNA). Under the action of enzymes, inosinic acid can be decomposed into hypoxanthine. More and more studies have confirmed that inosinic acid has flavor characteristics. In recent years, a large number of studies by domestic and foreign scholars have shown that the main material basis of the umami characteristics of chicken meat is determined by inosinic acid. How to solve the problem of how humans can enhance taste and appetite while absorbing rich nutrients has always been a historical responsibility of animal husbandry production.

[0003] Ma-feather broilers are a native broiler breed with a long history in my country. Compared with white-feather broilers, Ma-feather broilers are more suitable for Chinese cooking and are widely welcomed by consumers. The existing Ma-feather broiler breeding method has a complicated process, and the breeding direction tends to promote the growth and weight gain of Ma-feather broilers, but the improvement of chicken flavor and meat quality is not obvious. It is difficult to breed Ma-feather broilers with high inosinic acid content, resulting in a decrease in the quality and flavor of Ma-feather broilers, which cannot meet people's increasing requirements for chicken flavor and quality, and has a certain impact on economic benefits. Therefore, the present invention proposes a breeding method for Ma-feather broilers with high inosinic acid content to solve the problems existing in the prior art. Summary of the invention

[0004] In view of the above problems, the purpose of the present invention is to propose a breeding method for hemp-feather broilers with high inosinic acid content, so as to solve the problem that the existing hemp-feather broiler breeding methods do not significantly improve the flavor and quality of chicken, and it is difficult to breed hemp-feather broilers with high inosinic acid content, resulting in a decrease in the quality and flavor of hemp-feather broilers, and unable to meet people's increasingly higher requirements for chicken flavor and quality.

[0005] In order to achieve the purpose of the present invention, the present invention is implemented by the following technical scheme: a breeding method for high inosinic acid content hemp feather broiler chickens, comprising the following steps:

[0006] Step 1: The breast muscles and leg muscles of Hetian chicken, Wenchang chicken, Huxu chicken, Xianju chicken and Zhengyang Sanhuang chicken were collected for determination of inosinic acid content. The results showed that the inosinic acid content in the breast muscles and leg muscles of Hetian chicken was the highest. Therefore, Hetian chicken was selected as the male parent, and then Chongren Ma chicken with hemp feather trait and growth rate up to standard was selected as the female parent, and hybridized with the selected Hetian chicken male parent. After egg laying and hatching, female individuals with hemp feather trait were selected for seed keeping as F1 generation hemp feather broiler chickens;

[0007] Step 2: raising F1 generation hemp-feather broilers and selecting F1 generation hemp-feather broilers with growth rates that meet the standards as female parents, and then backcrossing the selected F1 generation hemp-feather broiler female parents with the Hetian chicken male parent selected in step 1, and after laying and hatching eggs, selecting female individuals with hemp-feather traits to keep as F2 generation hemp-feather broilers;

[0008] Step 3: raising F2 generation hemp-feather broilers and selecting F2 generation hemp-feather broilers with growth rate that meets the standard as female parents, and then collecting breast muscles and leg muscles of Qingyuan hemp chickens, Yinxiang hemp chickens, Luohe hemp chickens and Huaibei hemp chickens for inosinic acid content determination, and the results show that the inosinic acid content of breast muscles and leg muscles of Qingyuan hemp chickens is the highest. Therefore, Qingyuan hemp chickens with hemp-feather traits are selected as male parents, and hybridized with the selected F2 generation hemp-feather broiler female parents. After laying eggs and hatching, individuals with hemp-feather traits are selected for seed keeping as F3 generation hemp-feather broilers;

[0009] Step 4: Raise the F3 generation of hemp-feathered broilers and select male and female individuals that meet the growth rate standards for cross-crossing. After the eggs are laid and hatched, select individuals with hemp-feather traits for breeding as a new strain of hemp-feathered broilers with a high inosinic acid content.

[0010] A further improvement is that in the step 1, the selected Hetian chicken father is older than 200 days old and weighs more than 2.5 kg; in the step 3, the selected Qingyuan Ma chicken father is older than 200 days old and weighs more than 2.5 kg.

[0011] The further improvement lies in that: in the step 1, the specific steps of determining the inosinic acid content of the breast muscle and leg muscle of Hetian chicken, Wenchang chicken, bearded chicken, Xianju chicken and Zhengyang Sanhuang chicken are as follows: first, using a 5% perchloric acid solution to extract the inosinic acid in each muscle sample, then reacting it with a 0.5 mol / L sodium hydroxide solution to generate sodium inosinate, and finally detecting it by a high performance liquid chromatograph equipped with an ultraviolet detector, and using an external standard method for quantitative analysis to obtain the inosinic acid content value of each muscle sample.

[0012] A further improvement is that in step one, the age of the Chongren Ma chicken mother selected is 5 months, and the weight of the Chongren Ma chicken mother selected is greater than 1.25 kg, which means that the growth rate meets the standard.

[0013] A further improvement is that in the step 2, the selected F1 generation hemp-feather broiler mother is 5 months old, and the selected F1 generation hemp-feather broiler mother has a body weight greater than 1.25 kg, which means that the growth rate meets the standard.

[0014] A further improvement is that in step three, the selected F2 generation hemp-feather broiler mother is 5 months old, and the selected F2 generation hemp-feather broiler mother has a body weight greater than 1.30 kg, which means that the growth rate meets the standard.

[0015] The further improvement lies in that: in the step three, the specific steps of determining the inosinic acid content of the pectoral muscle and leg muscle of Qingyuan Ma chicken, Yinxiang Ma chicken, Luohe Ma chicken and Huaibei Ma chicken are as follows: first, use a 5% perchloric acid solution to extract the inosinic acid in each muscle sample, then react it with a 0.5 mol / L sodium hydroxide solution to generate sodium inosinate, and finally detect it by a high performance liquid chromatograph equipped with an ultraviolet detector, and use an external standard method for quantitative analysis to obtain the inosinic acid content value of each muscle sample.

[0016] A further improvement is that in step 4, the male and female F3 generation broilers selected are both 5 months old, and the weight of the male F3 generation broilers selected is greater than 1.35 kg, which means that the growth rate meets the standard, and the weight of the female F3 generation broilers selected is greater than 1.25 kg, which means that the growth rate meets the standard.

[0017] The beneficial effects of the present invention are as follows: the present invention first selects Hetian chicken and Chongren Ma chicken as the male and female parents respectively and hybridizes to obtain F1 generation Ma Yu broilers, then uses the F1 generation Ma Yu broilers as the female parent and backcrosses with the Hetian chicken male parent to obtain F2 generation Ma Yu broilers, then uses the F2 generation Ma Yu broilers as the female parent and selects Qingyuan Ma chicken as the male parent for hybridization to obtain F3 generation Ma Yu broilers, then selects male and female individuals with growth rates that meet the standards from the F3 generation Ma Yu broilers for cross-crossing and fixation, and finally obtains a new strain of Ma Yu broilers with high inosinic acid content, and through multiple generations of breeding, increases the inosinic acid content in the meat of Ma Yu broilers, and at the same time improves the advantage of fast growth rate to a certain extent, thereby improving the chicken quality and flavor of Ma Yu broilers to meet people's increasing requirements for chicken flavor and quality, and can bring good economic benefits to the broiler breeding industry. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0019] Figure 1 The present invention is a schematic diagram of the process of breeding a method for high-inosinic acid content hemp-feathered broiler chickens. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] See also Figure 1 This embodiment provides a breeding method for high-inosinic acid content hemp-feathered broiler chickens, comprising the following steps:

[0022] Step 1: Collect breast muscles and leg muscles of three yellow chickens of different varieties, including Hetian chicken, Wenchang chicken, bearded chicken, Xianju chicken and Zhengyang three yellow chicken, and then measure the inosinic acid content of each sample. The test results show that the inosinic acid content in the breast muscles and leg muscles of Hetian chickens is the highest. Therefore, a Hetian chicken rooster older than 200 days and weighing more than 2.5 kg is selected as the male parent, and then a Chongren hemp chicken with hemp feather traits and a growth rate that meets the standard is selected as the female parent (the age is 5 months and the weight is greater than 1.25 kg, which means the growth rate meets the standard). The female parent older than 6 months can be hybridized with the selected Hetian chicken male parent. After laying eggs and hatching, female individuals with hemp feather traits are selected for breeding as F1 generation hemp feather broilers;

[0023] About the selection of three yellow chicken breeds:

[0024] Hetian chicken is a high-quality chicken breed originating from Hetian Town, Changting County, Fujian Province. With its excellent quality and unique flavor, it has become one of the famous local and even national delicacies. It has the characteristics of tender meat, rich nutrition, delicious taste and attractive color. Therefore, in this embodiment, the breast muscle and leg muscle of Hetian chicken are selected for the determination of inosinic acid content and used as the male parent candidate;

[0025] Wenchang chicken is a local fine broiler breed in Hainan Province with a history of more than 400 years. It is famous for its unique flavor and nutritional value. It has the characteristics of sweet and tender meat, rich nutrition, high medicinal value, and good color, aroma, taste and shape. Therefore, in this example, Wenchang chicken breast muscle and leg muscle are selected for inosinic acid content determination and as the male parent candidate;

[0026] Bearded chicken is a kind of excellent local broiler chicken originating from Huiyang area of ​​Guangdong Province. It is famous for its large population, wide distribution, well-developed breast muscle, early maturity and easy fattening, and excellent meat quality. It has the characteristics of high nutritional value and delicious meat. Therefore, in this embodiment, bearded chicken breast muscle and leg muscle are selected for inosinic acid content determination and as a male parent candidate;

[0027] Xianju chicken is a famous local fine breed chicken in China, which has many commendable advantages, including tender meat, delicious taste, rich nutrition, strong adaptability, low feed consumption and excellent egg-laying performance. Therefore, the breast muscle and leg muscle of Xianju chicken were selected in this embodiment for the determination of inosinic acid content and as the paternal candidate;

[0028] Zhengyang Sanhuang Chicken is a local chicken breed originating from Zhengyang County, Henan Province. It is not only a Chinese national geographical indication product, but also enjoys the reputation of "medicinal chicken" and "Chinese precious chicken breed". It has the characteristics of delicious meat, high nutritional value, and suitable for mechanized cage breeding. Therefore, in this embodiment, the breast muscle and leg muscle of Zhengyang Sanhuang Chicken are selected for the determination of inosinic acid content and as the male parent candidate;

[0029] The specific steps of measuring the inosinic acid content of the breast muscle and leg muscle of Hetian chicken, Wenchang chicken, bearded chicken, Xianju chicken and Zhengyang Sanhuang chicken in this embodiment are as follows: first, using a 5% perchloric acid solution to extract the inosinic acid in each muscle sample, then reacting it with a 0.5 mol / L sodium hydroxide solution to generate sodium inosinate, and finally detecting it by a high performance liquid chromatograph equipped with an ultraviolet detector, and using an external standard method for quantitative analysis to obtain the inosinic acid content value of each muscle sample;

[0030] Step 2: raising F1 generation hemp-feather broilers and selecting F1 generation hemp-feather broilers with growth rate that meet the standard as female parents, with a month age of 5 months and a body weight greater than 1.25 kg, which means the growth rate meets the standard, and then backcrossing the selected F1 generation hemp-feather broiler female parents with the Hetian chicken male parent selected in step 1, and after laying eggs and hatching, selecting female individuals with hemp-feather traits to keep as F2 generation hemp-feather broilers;

[0031] Step 3: raising F2 generation hemp-feather broilers and selecting F2 generation hemp-feather broilers with growth rate that meets the standard as female parents, with an age of 5 months and a body weight greater than 1.30 kg, which means that the growth rate meets the standard, and then collecting breast muscles and leg muscles of hemp-feather chickens, including Qingyuan hemp chickens, Yinxiang hemp chickens, Luohe hemp chickens and Huaibei hemp chickens, and then measuring the inosinic acid content of each muscle sample. It was found that the inosinic acid content of the breast muscles and leg muscles of Qingyuan hemp chickens was the highest. Therefore, Qingyuan hemp chickens with hemp-feather traits, an age of more than 200 days, and a body weight of more than 2.5 kg were selected as male parents, and hybridized with the selected F2 generation hemp-feather broiler female parents. After laying eggs and hatching, individuals with hemp-feather traits were selected for seed preservation as F3 generation hemp-feather broilers;

[0032] About the selection of hemp feather chicken breeds:

[0033] Qingyuan Ma chicken is a high-quality chicken breed originating from Qingxin County, Qingyuan City, Guangdong Province. It is one of the top ten famous chickens in China. It has the characteristics of tender meat, high nutritional value and good nourishing effect. Therefore, in this embodiment, the breast muscle and leg muscle of Qingyuan Ma chicken are selected for the determination of inosinic acid content and used as the male parent candidate;

[0034] Yinxiang hemp chicken is a carefully selected local chicken breed with delicious meat, high nutritional value and strong adaptability. Therefore, the breast muscle and leg muscle of Yinxiang hemp chicken are selected in this embodiment for the determination of inosine content and as the male parent candidate;

[0035] Luohe Ma chicken is a specialty of Luohe City, Henan Province, and has national agricultural product geographical indication certification. It has the characteristics of excellent production performance, delicious and tender meat and high nutritional value. Therefore, in this embodiment, the breast muscle and leg muscle of Luohe Ma chicken are selected for the determination of inosine content and used as the male parent candidate;

[0036] Huaibei Ma chicken is a specialty poultry from Huaibei City, Anhui Province, with a long breeding history and excellent breed characteristics, and has the characteristics of tender and delicious meat, high nutritional value, strong adaptability, and heat and moisture resistance. Therefore, this example uses Huaibei Ma chicken breast muscle and leg muscle to determine the inosinic acid content and as a male parent candidate;

[0037] The specific steps of measuring the inosinic acid content of the breast and leg muscles of Qingyuan Ma chicken, Yinxiang Ma chicken, Luohe Ma chicken and Huaibei Ma chicken in this embodiment are as follows: first, using a 5% perchloric acid solution to extract the inosinic acid in each muscle sample, then reacting it with a 0.5 mol / L sodium hydroxide solution to generate sodium inosinate, and finally detecting it by a high performance liquid chromatograph equipped with an ultraviolet detector, and using an external standard method for quantitative analysis to obtain the inosinic acid content value of each muscle sample;

[0038] Step 4: Raise the F3 generation of hemp-feathered broilers and select male and female individuals that meet the growth rate standards for cross-breeding. The age of both individuals is 5 months. Male individuals with a body weight greater than 1.35kg meet the growth rate standards, and female individuals with a body weight greater than 1.25kg meet the growth rate standards. After egg laying and hatching, select individuals with hemp-feather traits for breeding as a new strain of hemp-feathered broilers with a high inosinic acid content.

[0039] The inosinic acid content of the breast muscle and leg muscle of the high-inosinic acid content bred in the embodiment of the present invention was tested, and the breast muscle and leg muscle of the broiler currently purchased on the market were used as a comparative example. The test results are shown in Table 1 below:

[0040] Table 1 Detection comparison table

[0041]

[0042] As can be seen from Table 1 above, the inosinic acid content of the hemp-feathered broilers bred using the breeding method of the present invention is significantly higher.

[0043] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for breeding broiler chickens with high inosinic acid content, characterized in that: The following steps are involved: Step 1: The breast muscles and leg muscles of Hetian chicken, Wenchang chicken, bearded chicken, Xianju chicken and Zhengyang Sanhuang chicken were collected for determination of inosinic acid content. The results showed that the inosinic acid content in the breast muscles and leg muscles of Hetian chicken was the highest. Therefore, Hetian chicken was selected as the male parent, and then Chongren Ma chicken with hemp feather trait and growth rate up to standard was selected as the female parent, and hybridized with the selected Hetian chicken male parent. After egg laying and hatching, female individuals with hemp feather trait were selected for seed keeping as F1 generation hemp feather broilers. The selected Hetian chicken male parent was older than 200 days old and weighed more than 2.5 kg. The selected Chongren Ma chicken female parent was 5 months old and weighed more than 1.25 kg, which means the growth rate was up to standard. Step 2: raising F1 generation hemp feather broiler chickens and selecting F1 generation hemp feather broiler chickens with growth rate that meet the standard as female parents, and then backcrossing the selected F1 generation hemp feather broiler female parents with the Hetian chicken male parent selected in step 1. After laying eggs and hatching, female individuals with hemp feather traits are selected for breeding as F2 generation hemp feather broiler chickens. The selected F1 generation hemp feather broiler female parents are 5 months old, and the selected F1 generation hemp feather broiler female parents have a weight of more than 1.25 kg, which means that the growth rate meets the standard; Step 3: raising F2 generation hemp-feather broilers and selecting F2 generation hemp-feather broilers with growth rate that meets the standard as the female parent, and then collecting the breast muscles and leg muscles of Qingyuan hemp chicken, Yinxiang hemp chicken, Luohe hemp chicken and Huaibei hemp chicken for inosinic acid content determination, and the results show that the inosinic acid content of the breast muscles and leg muscles of Qingyuan hemp chicken is the highest, so Qingyuan hemp chicken with hemp feather trait is selected as the male parent, and hybridized with the selected F2 generation hemp feather broiler female parent, after laying eggs and hatching, individuals with hemp feather trait are selected for seed retention as F3 generation hemp feather broilers, the selected Qingyuan hemp chicken male parent is older than 200 days old and weighs more than 2.5kg, the selected F2 generation hemp feather broiler female parent is 5 months old, and the selected F2 generation hemp feather broiler female parent weighs more than 1.30kg, which means that the growth rate meets the standard; Step 4: Raise the F3 generation of hemp-feather broilers and select male and female individuals that meet the growth rate standards for cross-crossing. After egg laying and hatching, select individuals with hemp-feather traits for breeding as a new strain of hemp-feather broilers with high inosinic acid content. The male and female F3 generation hemp-feather broilers selected are both 5 months old. The weight of the male F3 generation hemp-feather broilers selected is greater than 1.35kg, which means that the growth rate standards are met. The weight of the female F3 generation hemp-feather broilers selected is greater than 1.25kg, which means that the growth rate standards are met.

2. The method for breeding high inosinic acid content broiler chickens according to claim 1, characterized in that: In the step 1, the specific steps of determining the inosinic acid content of the breast muscle and leg muscle of Hetian chicken, Wenchang chicken, bearded chicken, Xianju chicken and Zhengyang Sanhuang chicken are as follows: first, use a 5% perchloric acid solution to extract the inosinic acid in each muscle sample, then react it with a 0.5 mol / L sodium hydroxide solution to generate sodium inosinate, and finally detect it by a high performance liquid chromatograph equipped with an ultraviolet detector, and use an external standard method for quantitative analysis to obtain the inosinic acid content value of each muscle sample.

3. The method for breeding high inosinic acid content broiler chickens according to claim 1, characterized in that: In the step three, the specific steps of determining the inosinic acid content in the pectoral muscles and leg muscles of Qingyuan Ma chicken, Yinxiang Ma chicken, Luohe Ma chicken and Huaibei Ma chicken are as follows: first, use a 5% perchloric acid solution to extract the inosinic acid in each muscle sample, then react it with a 0.5 mol / L sodium hydroxide solution to generate sodium inosinate, and finally detect it by a high performance liquid chromatograph equipped with an ultraviolet detector, and use the external standard method for quantitative analysis to obtain the inosinic acid content value of each muscle sample.

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