A feed additive for improving the polysaccharide in the body wall of sea cucumbers, its preparation method and application

Abstract

The present invention discloses a feed additive for improving the polysaccharide in the body wall of sea cucumbers, its preparation method and application, belonging to the technical field of functional biological additives for aquatic compound feeds. It comprises the following components in parts by weight: 0-2 parts of fucose, 1-3 parts of glucose, and 5-7 parts of fucoidan. The preparation method of the present invention is simple and has obvious effects, extremely significantly improving the content of polysaccharide in the body wall of sea cucumbers and increasing the commercial value of sea cucumbers. The present invention is a mixture of three sugars, which has a certain effect of promoting the growth and enhancing the immunity of sea cucumbers.

Description

Technical Field

[0001] The present invention relates to the technical field of functional biological additives for aquatic compound feeds, and particularly to a feed additive for increasing the polysaccharide in the body wall of sea cucumbers, its preparation method and application. Background Art

[0002] Sea cucumbers are the species with the highest nutritional and economic value in the class Holothuroidea. In recent years, studies have found that sea cucumber polysaccharides are one of the main functional substances, mainly including two types: holothurian glycosaminoglycan or acidic mucopolysaccharide (HGAG) and fucoidan (HF). HGAG contains a large amount of sulfate groups and fucose, and is highly fucosylated chondroitin sulfate (FuCS), which has biological functional activities such as anticoagulation, anti-tumor, and antioxidant, and is closely related to the biological activity functions of sea cucumbers.

[0003] The in vivo synthesis of sea cucumber polysaccharides is affected by the supply of glycosyl and sulfonyl groups and the activity of polysaccharide synthase. The abundant SO42- in seawater ensures the synthesis of sulfonyl donors, while the abundance of fucose in organisms is low and the synthesis pathway is complex. Its donor, guanosine diphosphate-fucose (GDP-fucose), is one of the limiting factors for the synthesis of sea cucumber polysaccharides.

[0004] Currently, during the cultivation of sea cucumbers, carbohydrate additives (such as dextran, chitosan oligosaccharide, etc.) have been used, but most of them are used as immune enhancers or growth promoters, and have not yet been involved in the field of increasing the polysaccharide content in the body wall of sea cucumbers. Therefore, how to increase the polysaccharide content in the body wall of sea cucumbers is an urgent problem to be solved at present. Summary of the Invention

[0005] The purpose of the present invention is to provide a feed additive for increasing the polysaccharide in the body wall of sea cucumbers, its preparation method and application, so as to solve the problems existing in the above-mentioned prior art.

[0006] To achieve the above purpose, the present invention provides the following solutions:

[0007] The present invention provides a feed additive for increasing the polysaccharide in the body wall of sea cucumbers, comprising the following components in parts by weight: 0-2 parts of fucose, 1-3 parts of glucose, and 5-7 parts of fucoidan.

[0008] Preferably, it comprises the following components in parts by weight: 1 part of fucose, 2 parts of glucose, and 6 parts of fucoidan.

[0009] The present invention also provides a preparation method of the feed additive, weighing the raw materials and carrageenan according to the mass ratio, adding water to adjust to a thick state, heating, cooling to form a solid block, drying, cooling, and grinding into powder, which is the feed additive.

[0010] Preferably, the mass ratio of the raw materials to carrageenan is 1:1.

[0011] Preferably, the heating is water bath heating at a temperature of 90°C for 15 minutes.

[0012] Preferably, the drying temperature is 75°C.

[0013] Preferably, after grinding, it is sieved through a 40-mesh sieve, and the material on the sieve is ≤1%.

[0014] The present invention also provides a feed for increasing the polysaccharide in the body wall of sea cucumbers, including the feed additive.

[0015] Preferably, the addition amount of the feed additive is 0.25%.

[0016] The present invention also provides a method for increasing the polysaccharide in the body wall of sea cucumbers, using the feed to feed sea cucumbers.

[0017] Preferably, the feed is started to be used 2 months before the end of the sea cucumber cultivation period.

[0018] Based on the above technical solutions, the present invention has the following technical effects:

[0019] 1. The present invention is simple to manufacture, with obvious effects, extremely significantly increasing the content of polysaccharide in the body wall of sea cucumbers and increasing the commercial value of sea cucumbers.

[0020] 2. The present invention is a mixture of three sugars, which has a certain effect on promoting the growth and enhancing the immunity of sea cucumbers. Detailed Embodiments

[0021] Now, various exemplary embodiments of the present invention will be described in detail. In the embodiments, the methods are all conventional methods unless otherwise specified, and the reagents are all conventional commercially available reagents or reagents prepared by conventional methods unless otherwise specified. This detailed description should not be considered as a limitation of the present invention, but should be understood as a more detailed description of certain aspects, characteristics and implementation schemes of the present invention.

[0022] It should be understood that the terms described in the present invention are only for describing specific embodiments and are not used to limit the present invention. In addition, for the numerical ranges in the present invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Each intermediate value within any stated value or stated range, as well as each smaller range between any other stated value or intermediate value within the stated range, is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded from the range.

[0023] Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Although this invention only describes preferred methods and materials, any methods and materials similar or equivalent to those described herein can also be used in the practice or testing of this invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials related to the documents. In case of conflict with any incorporated document, the content of this specification shall prevail.

[0024] Without departing from the scope or spirit of this invention, various improvements and changes can be made to the specific embodiments of the specification of this invention, which are obvious to those skilled in the art. Other embodiments obtained from the specification of this invention are obvious to those skilled in the art. The specification and examples of this invention are merely exemplary.

[0025] Regarding the use of "comprising", "including", "having", "containing", etc. herein, they are all open-ended terms, meaning including but not limited to.

[0026] The technical solutions described in this invention, unless otherwise specified, are all conventional solutions in the art. The reagents or raw materials used, unless otherwise specified, are all purchased from commercial channels or are already publicly available.

[0027] In the examples of this invention, fucose is commercially available with a purity ≥ 85%; glucose is commercially available with a purity ≥ 95%; fucoidan is commercially available with a purity ≥ 95%.

[0028] This invention is obtained based on the following experiments:

[0029] Different gradients of glucose (0, 0.5%, 1.0%, 1.5%, 2.0%), fucose (0, 0.25%, 0.75%), and fucoidan (0, 0.5%, 1.0%, 1.5%, 2.0%, 2.5%) were respectively added to the compound feed for sea cucumbers, and the sea cucumbers were fed for 60 days.

[0030] Table 1: Contents of total sugar and acidic mucopolysaccharide in the body wall of sea cucumbers

[0031]

[0032] Based on the GDP-fucose synthesis pathway in sea cucumbers, this invention provides a preparation capable of increasing the polysaccharide content in the body wall of sea cucumbers and its preparation and use method. Specifically, a sugar combination capable of increasing the polysaccharide content in the body wall of sea cucumbers is screened through experiments as a functional feed additive, so that it has the effect of increasing the polysaccharide content in the body wall of sea cucumbers, thereby improving the nutritional and health care and commercial value of sea cucumbers.

[0033] Example 1

[0034] A functional feed additive for increasing the polysaccharide in the body wall of sea cucumbers, which is composed of the following components in parts by weight: 1 part of fucose, 2 parts of glucose, and 6 parts of fucoidan.

[0035] The preparation method is as follows:

[0036] Weigh 3 raw materials according to the mass ratio, mix them thoroughly, then weigh carrageenan, mix it with the raw materials according to the mass ratio of raw materials to carrageenan of 1:1, add an appropriate amount of water to make it thick, heat and stir in a water bath at 90 °C for 15 min, dry it into a solid block with a freeze dryer after cooling, then dry it at 75 °C, grind it into powder after cooling, pass through a 40-mesh standard sieve, and the material on the sieve is ≤1%. The 24-hour dissolution rate is measured to be 45%.

[0037] Example 2

[0038] A functional feed additive for increasing the polysaccharide in the body wall of sea cucumbers, which is composed of the following components in parts by weight: 2 parts of fucose, 1 part of glucose, and 7 parts of fucoidan.

[0039] Weigh 3 raw materials according to the mass ratio, mix them thoroughly, then weigh carrageenan, mix it with the raw materials according to the mass ratio of raw materials to carrageenan of 1:1, add an appropriate amount of water to make it thick, heat and stir in a water bath at 90 °C for 15 min, dry it into a solid block with a freeze dryer after cooling, then dry it at 75 °C, grind it into powder after cooling, pass through a 40-mesh standard sieve, and the material on the sieve is ≤1%. The 24-hour dissolution rate is measured to be 45%.

[0040] Example 3

[0041] A functional feed additive for increasing the polysaccharide in the body wall of sea cucumbers, which is composed of the following components in parts by weight: 3 parts of glucose and 5 parts of fucoidan.

[0042] Weigh 2 raw materials according to the mass ratio, mix them thoroughly, then weigh carrageenan, mix it with the raw materials according to the mass ratio of raw materials to carrageenan of 1:1, add an appropriate amount of water to make it thick, heat and stir in a water bath at 90 °C for 15 min, dry it into a solid block with a freeze dryer after cooling, then dry it at 75 °C, grind it into powder after cooling, pass through a 40-mesh standard sieve, and the material on the sieve is ≤1%. The 24-hour dissolution rate is measured to be 45%.

[0043] Test example

[0044] Mix the functional compound feed additive produced in Example 1 with sea cucumbers according to the ratio of feed to additive of 400:1. The results are shown in Table 2.

[0045] Feed sea cucumbers with commercially available conventional feed for 60 days. The total sugar content in the body wall is 17.54%, the content of acidic mucopolysaccharide is 11.87%, the weight gain rate is 27.51%, the lysozyme activity in the coelomic fluid is 161.24 U / mg prot, and the superoxide dismutase activity is 38.81 U / mg prot.

[0046] The functional compound feed additive prepared in Example 1 of the present invention was mixed into the conventional feed and fed to sea cucumbers for 60 days. The total sugar content in the body wall was 26.45%, the acidic mucopolysaccharide content was 19.54%, the weight gain rate was 63.24%, the lysozyme activity was 179.33 U / mg prot, and the superoxide dismutase activity was 62.87 U / mg prot.

[0047] Table 2: Total sugar and acidic mucopolysaccharide contents in the body wall of sea cucumbers

[0048]

[0049] Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the present invention, rather than limitations on the implementation manners of the present invention. For those of ordinary skill in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to enumerate all the implementation manners here. Any modifications, equivalent replacements, and improvements made within the spirit and principle of the present invention shall be included within the protection scope of the claims of the present invention.

Claims

1. A feed additive for increasing the polysaccharide content of sea cucumber body wall, characterized in that: The invention comprises the following components in parts by weight: 1 part of fucose, 2 parts of glucose and 6 parts of fucoidan; the sea cucumber body wall polysaccharide comprises acid mucopolysaccharide.

2. The method for preparing the feed additive according to claim 1, characterized in that: The raw materials are weighed and mixed with carrageenan according to the mass ratio, water is added to make it thick, heated, cooled to form solid blocks, dried, cooled and ground into powder to obtain the feed additive.

3. The preparation method according to claim 2, characterized in that: The mass ratio of the raw material to carrageenan is 1:

1.

4. The preparation method according to claim 2, characterized in that: The heating is water bath heating at 90° C. for 15 min.

5. The preparation method according to claim 2, characterized in that: The drying temperature is 75°C.

6. The preparation method according to claim 2, characterized in that: After the powder is ground, it is passed through a 40-mesh sieve, and the material on the sieve is ≤1%.

7. A feed for increasing the polysaccharide content of sea cucumber body wall, characterized in that: Including the feed additive according to claim 1.

8. The feed according to claim 7, characterized in that The added amount of the feed additive is 0.25%.

9. A method for increasing the polysaccharide content of sea cucumber body wall, characterized in that: The sea cucumber is fed with the feed according to claim 7, wherein the sea cucumber body wall polysaccharide comprises acidic mucopolysaccharide.

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