Application of spectrum and light intensity in promoting increase of number of thorns of stichopus japonicus

Abstract

The invention relates to an application of spectrum and light intensity in promoting increase of the number of thorns of stichopus japonicus, and belongs to the field of aquaculture. The application method comprises the following steps: culturing young stichopus japonicus under indoor culture conditions; the illumination period is set to be 12L: 12D circulation; in the culture process, blue light is adopted for irradiation, the wavelength of the blue light is 440-490 nm, and the illumination intensity is 1500 lx. According to the method, the technical effect of increasing the number of the thorns of the stichopus japonicus in the indoor environment is achieved by adopting the blue light irradiation with the specific wavelength and illumination intensity in the stichopus japonicus breeding process and combining the proper illumination period.

Description

Technical Field

[0001] The invention belongs to the field of aquaculture, and in particular relates to a breeding method for promoting the growth of the number of sea cucumber spines by regulating spectrum and light intensity. Background Art

[0002] Sea cucumber (Apostichopus japonicus) belongs to the phylum Echinodermata, class Holothuroidea. It is mainly distributed in the northern coastal areas of China, Japan, North Korea, and the Russian Far East. It grows in temperate shallow sea areas, usually inhabiting shallow sea areas with a depth of 3 to 15 meters, and preferably inhabiting the bottom of reefs or fine sand and mud with less fluctuations and rich seaweed. Due to its excellent nutritional value and medicinal function, sea cucumber has long been regarded as a precious food and is deeply favored by consumers. Due to its high economic value, it has become an important marine aquaculture species in my country in recent years. The breeding methods of sea cucumbers in my country mainly include pond breeding, cofferdam breeding, shallow seabed seeding breeding, hanging cage breeding, factory breeding, etc. Factory breeding of sea cucumbers has the advantages of controllable environment, short breeding cycle, less disease outbreak, and high economic benefits of breeding. However, how to set reasonable lighting conditions and adopt reasonable feeding strategies in factory breeding production has become a problem of concern.

[0003] As a typical echinoderm, sea cucumbers have spines as their distinctive appearance feature. The spines are a protruding tissue on the surface of the sea cucumber, usually distributed on its back and sides. The size, shape and distribution of these spines can change with the growth and development of the sea cucumber and changes in environmental factors. Although sea cucumbers lack typical photoreceptor organs, they can still sense changes in light intensity, photoperiod and spectrum, and adapt to these changes through cytochromes and photoreceptors distributed in spines, tube feet, body wall and nervous system.

[0004] Light is a relatively complex external ecological factor, including spectrum, light intensity and photoperiod, which has a direct or indirect impact on the life activities of aquatic animals. Sea cucumbers have certain light-avoiding behaviors and are highly sensitive to changes in light intensity. They prefer low-light environments. When the light is too strong, they often hide in the shade and appear to be in a contracted state under strong light. Sea cucumbers are active at night or in low-light conditions and eat a lot. Light is a key environmental factor affecting the growth and behavior of sea cucumbers. Its light intensity and photoperiod fluctuate with changes in water depth and time, so it may have a significant impact on the development and behavior of sea cucumbers. Although indoor shading measures are generally adopted in current artificial breeding practices, there is still no clear conclusion on the most suitable specific parameters such as light intensity, photoperiod and spectral composition. Summary of the invention

[0005] The object of the present invention is to overcome the deficiencies of the prior art and provide a method for significantly increasing the number of spines on the body surface of sea cucumbers through light irradiation, thereby improving the appearance quality of sea cucumbers and enhancing their market value. Light is one of the environmental factors affecting the growth of sea cucumbers. Both the spectrum and light intensity are adjustable factors in the light conditions. Therefore, the present invention realizes the technical effect of increasing the number of spines of sea cucumbers in an indoor environment by using blue light with a specific wavelength and light intensity during the cultivation of sea cucumbers, and combining with an appropriate light cycle.

[0006] The technical solution of the present invention is as follows:

[0007] Application of spectrum and light intensity in promoting the growth of the number of spines of sea cucumbers. The method of the application is: cultivating juvenile sea cucumbers under indoor cultivation conditions; setting the light cycle as a 12L:12D cycle; using blue light irradiation during the cultivation process, the wavelength of the blue light is 440 - 490 nm, and the light intensity is 1500 lx.

[0008] Furthermore, the sea cucumbers are 10 - 20 g, and the depth of the cultivation water body is 80 - 120 cm.

[0009] Furthermore, the duration of using blue light irradiation is more than 15 days.

[0010] Advantages of the present invention compared with the prior art:

[0011] (1) The 1500 lx blue light band adopted by the present invention can increase the number of spines of sea cucumbers.

[0012] (2) The method of the present invention is simple and easy to implement. Only by appropriately illuminating the cultivation pond with blue light, the effect can be achieved without changing the feed formula and water quality conditions, and it has significant economic benefits and broad application prospects. Specific embodiments

[0013] The technical solutions in the embodiments of the present invention will be clearly and completely described below. The following described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. The examples given do not limit the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts fall within the scope of protection of the present invention.

[0014] The experimental methods used in the following embodiments are all conventional methods unless otherwise specified.

[0015] The materials, reagents, etc. used in the following embodiments can be obtained from commercial channels unless otherwise specified.

[0016] Example 1

[0017] Application of spectrum and light intensity in promoting the increase of the number of spines of Apostichopus japonicus, and the said application comprises the following steps. Regulation of spectrum: First, set the light cycle as 12D:12L, and the spectra are respectively yellow light (570 - 600 nm), blue light (440 - 490 nm), green light (510 - 550 nm), red light (630 - 670 nm), composite light (a composite light of red light, blue light and green light), a completely dark group and a control group.

[0018] Select healthy Apostichopus japonicus with a body weight of about 15 g and about 34 spines. During the experiment, the aquaculture tanks are wrapped with black plastic sheets, and the upper part of the completely dark experimental tank is covered with a black plastic film, and the irradiation lamp is the only light source. During the experiment, the water is changed every other day, and at the same time, the bottom is sucked, the water depth is 1 m, and the water change amount is 1 / 2 of the aquaculture water body. The experiment lasts for 60 days, and the number of spines is counted every 15 days. The spectrum experiment is divided into seven groups: the normal natural light environment is the control group, and there are six different treatment groups of darkness, yellow light (570 - 600 nm), blue light (440 - 490 nm), green light (510 - 550 nm), red light (630 - 670 nm) and composite light (a composite light of red light, blue light and green light).

[0019] On the 15th day of illumination, there was a significant difference in the number of spines of the Apostichopus japonicus in the blue light group compared with other experimental groups and the control group (P < 0.05), and the increase in the number of spines was significantly higher than that of other experimental groups and the control group (P < 0.05); on the 30th day and the 45th day of illumination, the number of spines of the Apostichopus japonicus in the blue light group was significantly more than that of the control group, the yellow light group, the green light group, the composite light group and the red light group (P < 0.05), and was extremely significantly more than that of the dark group (P < 0.01) (Table 1). It can be concluded that the increase in the number of spines of Apostichopus japonicus requires illumination, and the group with the most increase in the number of spines of Apostichopus japonicus is the blue light group.

[0020] Table 1 Changes in the number of spines of Apostichopus japonicus under illumination with different spectra for 60 days

[0021]

[0022] Note: Different superscript letters for the data in the same column indicate significant differences between groups (P < 0.05).

[0023] Illumination conditions with different spectra had no significant effect on the weight gain of Apostichopus japonicus, and there was no significant difference in weight change between each experimental group and the control group (P > 0.05) (Table 2), indicating that blue light is directly beneficial to the increase in the number of spines of Apostichopus japonicus rather than through an indirect effect on the growth of Apostichopus japonicus.

[0024] Table 2 Changes in the weight of Apostichopus japonicus under illumination with different spectra for 60 days

[0025]

[0026] Example 2

[0027] A method for promoting the growth of the number of spines of Apostichopus japonicus by regulating the spectrum and light intensity of the present invention includes the following steps. Regulation of the spectrum: First, set the light cycle to 12D:12L, the spectrum to blue light (440 - 490 nm), and the light intensities to 1500 lx, 3000 lx, and 4500 lx respectively.

[0028] Select healthy Apostichopus japonicus with a body weight of about 15 g and about 34 spines. During the experiment, the aquaculture tank was wrapped with black plastic sheeting, and the top of the completely dark experimental tank was covered with a black plastic film. The irradiation lamp was the only light source. During the experiment, the water was changed every other day, and the bottom was siphoned each time. The amount of water changed was 1 / 2 of the aquaculture water body. The experiment was carried out for 60 days, and the number of spines was counted every 15 days. The light intensity experiment was divided into four groups: the normal natural light environment was the control group, and the three different treatment groups with light intensities of 1500 lx, 3000 lx, and 4500 lx.

[0029] The number of spines of Apostichopus japonicus in the two groups with light intensities of 1500 lx and 3000 lx was significantly higher than that in the 4500 lx group and the control group (P < 0.05); at the 30th day, 45th day, and 60th day of illumination with different light intensities, the number of spines of Apostichopus japonicus in the 1500 lx group was significantly more than that in the control group and the 4500 lx group (P < 0.05) (Table 3). When the light intensity was 1500 lx, the number of spines of Apostichopus japonicus increased the most, followed by 3000 lx. However, when the light intensity was 4500 lx, the increase in the number of spines of Apostichopus japonicus was not significantly different from that of the control group (P > 0.05).

[0030] Table 3 Changes in the number of spines of Apostichopus japonicus under blue light illumination with different light intensities for 60 days

[0031]

[0032] Note: Different superscript letters for the data in the same column indicate significant differences between groups (p < 0.05).

[0033] Under the conditions of blue light with different light intensities, there was no significant effect on the body weight gain of Apostichopus japonicus (P > 0.05) (Table 4), indicating that 1500 lx blue light is directly beneficial to the growth of the number of spines of Apostichopus japonicus rather than through an indirect effect on the growth of Apostichopus japonicus.

[0034] Table 4 Changes in the body weight of Apostichopus japonicus under blue light illumination with different light intensities for 60 days

[0035]

Claims

1. Application of spectrum and light intensity in promoting the growth of sea cucumber spines, characterized in that: The application method is as follows: cultivating young sea cucumbers under indoor breeding conditions; setting the light cycle to a 12L:12D cycle; and using blue light irradiation during the cultivation process, wherein the wavelength of the blue light is 440-490nm and the light intensity is 1500lx.

2. The application of the spectrum and light intensity according to claim 1 in promoting the growth of the number of sea cucumber spines, characterized in that: The sea cucumber weighs 10-20g, and the depth of the breeding water is 80-120cm.

3. The application of the spectrum and light intensity according to claim 1 in promoting the growth of the number of sea cucumber spines, characterized in that: The duration of the blue light irradiation is more than 15 days.

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