A composition for rapidly recovering sea cucumber from stress state and a use method thereof

Abstract

The present application relates to a kind of composition and use method for making sea cucumber from stress state fast recovery, it is characterized in that, the composition is composed of taurine and lactobacillus plantarum.The mass fraction ratio of taurine and lactobacillus plantarum is 1:2 optimally, the composition of the present application is healthy and safe, non-toxic and harmless to sea cucumber, and can make the sea cucumber in stress state in 1-3min recovery to normal living state.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of aquaculture, and particularly relates to a composition for quickly recovering sea cucumber from stress state and a use method. BACKGROUND

[0002] In the process of sea cucumber cultivation, the cultivation production unit is prone to make sea cucumber stressed when performing operations such as dividing seedlings, changing pools, and changing water, and sea cucumber appears conditions such as rigid body, cage hanging, greatly reduced food intake, and intestinal vomiting, which affects the normal growth and development of sea cucumber, and long-time stress state can lead to decreased immunity of sea cucumber and more easily cause large-scale serious diseases or death. At present, common anti-stress means of the cultivation production unit include controlling and maintaining stable water environment, applying bottom modifier, detoxifying agent, and compound vitamin, etc. However, these methods have disadvantages such as dependence on experience of cultivation personnel, tedious daily management, and long time for effect. SUMMARY

[0003] The technical problem to be solved by the present application is to provide a composition for quickly recovering sea cucumber from stress state and a use method, which can quickly recover sea cucumber from rigid and contracted state in stress state to a state of natural and relaxed meat spines.

[0004] In order to solve the above technical problem, the technical scheme adopted by the present application is as follows:

[0005] A composition for quickly recovering sea cucumber from stress state, which is composed of taurine and lactobacillus plantarum.

[0006] The mass fraction ratio of the taurine and the lactobacillus plantarum is 0.5-1:0.5-2, and the taurine and the lactobacillus plantarum are both market products.

[0007] Further, the optimal mass fraction ratio of the taurine and the lactobacillus plantarum is 1:2.

[0008] The present application also provides a use method of the composition. After sea cucumber produces stress reaction, the composition is mixed with seawater at a ratio of 3:4000 to form a soaking liquid, sea cucumber is recovered to a normal living state, the soaking liquid can be sprayed to a cultivation pond at a ratio of 1:120, and the method is for the purpose of non-disease treatment and prevention.

[0009] The present application has the following beneficial effects compared with the prior art: the composition of the present application is healthy and safe, has no toxicity and harm to sea cucumber, and can recover sea cucumber in stress state to a normal living state within 1-3 minutes. DETAILED DESCRIPTION

[0010] The technical scheme of the present application is further explained by the following examples, but the protection scope of the present application is not limited in any form by the examples.

[0011] The raw materials used in the examples are all obtained by commercial means.

[0012] Taurine, purchased from Zhengzhou Best Food Additives Co., Ltd., production batch: 20230315.

[0013] Lactobacillus plantarum, purchased from Jiangsu Nake Biological Engineering Co., Ltd., production batch: 20230210.

[0014] Example 1

[0015] The composition for quickly recovering sea cucumber from stress state is prepared by the present application, and the composition is made of the following components in a weight ratio: taurine 1 part, Lactobacillus plantarum 2 parts.

[0016] Application: 3g of the composition is mixed with 4L of seawater to form a soaking solution. 75 healthy sea cucumbers (body weight 2.23±0.24g) are selected and randomly divided into 3 groups. After the sea cucumbers are taken out of the water for 20 minutes, wait for the sea cucumbers to produce stress reaction after being taken out of the water, the body is rigid, and the water is presented. Then soak the sea cucumbers that produce water stress reaction in the soaking solution, observe the recovery state of the sea cucumbers and time.

[0017] Example 2

[0018] The composition for quickly recovering sea cucumber from stress state is prepared by the present application, and the composition is made of the following components in a weight ratio: taurine 0.5 parts, Lactobacillus plantarum 0.5 parts.

[0019] Application: 1g of the composition is mixed with 4L of seawater to form a soaking solution. 75 healthy sea cucumbers (body weight 2.23±0.24g) are selected and randomly divided into 3 groups. After the sea cucumbers are taken out of the water for 20 minutes, wait for the sea cucumbers to produce stress reaction after being taken out of the water, the body is rigid, and the water is presented. Then soak the sea cucumbers that produce water stress reaction in the soaking solution, observe the recovery state of the sea cucumbers and time.

[0020] Example 3

[0021] The composition for quickly recovering sea cucumber from stress state is prepared by the present application, and the composition is made of the following components in a weight ratio: taurine 0.5 parts, Lactobacillus plantarum 2 parts.

[0022] 2.5g of the composition is mixed with 4L of seawater to form a soaking solution. 75 healthy sea cucumbers (body weight 2.23±0.24g) are selected and randomly divided into 3 groups. After the sea cucumbers are taken out of the water for 20 minutes, wait for the sea cucumbers to produce stress reaction after being taken out of the water, the body is rigid, and the water is presented. Then soak the sea cucumbers that produce water stress reaction in the soaking solution, observe the recovery state of the sea cucumbers and time.

[0023] Example 4

[0024] The composition for quickly recovering sea cucumber from stress state is made of the following components in the weight ratio: 1 part of taurine, 0.5 parts of Lactobacillus plantarum.

[0025] 1.5 g of the composition is mixed with 4 L of seawater to form a soaking solution. 75 healthy sea cucumbers (2.23±0.24 g in weight) are selected and randomly divided into 3 groups. The sea cucumbers are taken out of water and left for 20 min, waiting for the sea cucumbers to produce stress reaction after being taken out of water, with the body rigid and in a shrunk state. Then the sea cucumbers producing stress reaction after being taken out of water are soaked in the soaking solution, and the recovery state of the sea cucumbers is observed and the time is recorded.

[0026] Comparative Example 1

[0027] 1 g of taurine is mixed with 4 L of seawater to form a soaking solution.

[0028] 75 healthy sea cucumbers (2.23±0.24 g in weight) are selected and randomly divided into 3 groups. The sea cucumbers are taken out of water and left for 20 min, waiting for the sea cucumbers to produce stress reaction after being taken out of water, with the body rigid and in a shrunk state. Then the sea cucumbers producing stress reaction after being taken out of water are soaked in the soaking solution, and the recovery state of the sea cucumbers is observed and the time is recorded.

[0029] Comparative Example 2

[0030] 2 g of Lactobacillus plantarum is mixed with 4 L of seawater to form a soaking solution.

[0031] 75 healthy sea cucumbers (2.23±0.24 g in weight) are selected and randomly divided into 3 groups. The sea cucumbers are taken out of water and left for 20 min, waiting for the sea cucumbers to produce stress reaction after being taken out of water, with the body rigid and in a shrunk state. Then the sea cucumbers producing stress reaction after being taken out of water are soaked in the soaking solution, and the recovery state of the sea cucumbers is observed and the time is recorded.

[0032] Comparative Example 3

[0033] 75 healthy sea cucumbers (2.23±0.24 g in weight) are selected and randomly divided into 3 groups. The sea cucumbers are taken out of water and left for 20 min, waiting for the sea cucumbers to produce stress reaction after being taken out of water, with the body rigid and in a shrunk state. Then the sea cucumbers producing stress reaction after being taken out of water are soaked in the soaking solution, and the recovery state of the sea cucumbers is observed and the time is recorded.

[0034] The average recovery time of sea cucumbers in Examples 1, 2, 3, 4 and Comparative Examples 1, 2, 3 is 1.79±0.14 min, 2.29±0.17 min, 1.92±0.10 min, 2.40±0.13 min, 2.87±0.11 min, 2.76±0.16 min, and 3.85±0.15 min, respectively.

[0035] The results show that taurine and lactobacillus plantarum can relieve the stress response of sea cucumber, and the composition has good effect on eliminating the stress response such as poor stretching and poor feeding of sea cucumber after being poured into a pool and being changed water.

[0036] The embodiments of the present application only list the lactobacillus plantarum purchased from Jiangsu Nake Biological Engineering Co., Ltd. and the taurine purchased from Zhengzhou Best Food Additives Co., Ltd., and it is found in the development process that other strains of lactobacillus plantarum and taurine can also obtain the same technical effect.

Claims

1. A composition for rapidly recovering a sea cucumber from a stressed state, characterized in that, The composition is composed of taurine and lactobacillus plantarum, and the mass fraction ratio of the taurine and the lactobacillus plantarum is 1:

2.

2. The method of using the composition of claim 1, wherein, When the sea cucumber produces a stress response, the composition is mixed with seawater at a ratio of 3g:4000ml to form a soaking liquid, the sea cucumber is restored to a normal living state, and the soaking liquid is sprayed into the culture pond at a ratio of 1:120, and the method is for the purpose of non-disease treatment and prevention.