Burkholderia HSY-3 and its application

Abstract

The present invention belongs to the field of microbial engineering, and specifically relates to a Burkholderia HSY-3 and its application. The present invention screens out bacteria suitable for uranium mine remediation by constructing a separation, screening and culture system in different environments. The microorganisms provided by the present invention can significantly reduce the cost of restoration, while restoring the activity of microbial communities, and providing long-term environmental stability and environmental resistance.

Description

Technical Field

[0001] The invention belongs to the field of microbial engineering, and in particular relates to Burkholderia HSY-3 and application thereof. Background Art

[0002] At present, uranium mining and smelting mainly adopt three production processes: acid leaching, neutral leaching and alkaline leaching. Microbial remediation technology is used to carry out in-situ remediation of the mobile and diffusible uranium in the seepage water / groundwater of the natural radioactive waste generated by different production processes. Recent studies have confirmed that there is a close interaction between microorganisms and uranium. Through the adsorption of surface groups of microbial cells, the release of electrons from intracellular and extracellular polymers, and the mineralization of biological metabolites, various complex microbial remediation functions can make uranium from mobile U 6+ Converted to stable U 4+ , thus achieving in-situ remediation of uranium. However, for the seepage water / groundwater in the natural radioactive waste generated by uranium mines, due to the complex ion environment and exposure to radionuclides, traditional microbial agents may not be able to survive directly in the current system, resulting in the microbial remediation effect failing to meet expectations. Summary of the invention

[0003] To solve the above problems, the present invention provides a Burkholderia sp. HSY-3, which was deposited in the General Microbiological Center of China National Microbiological Culture Collection Committee on January 26, 2024. The address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC NO.29789.

[0004] The present invention also provides a bacterial agent, wherein the bacterial agent components include the above-mentioned Burkholderia HSY-3.

[0005] On the other hand, the present invention provides the use of Burkholderia HSY-3 in uranium mine pollution control.

[0006] The present invention also provides application of the above-mentioned bacterial agent in uranium mine pollution control.

[0007] The present invention also provides a method for treating uranium mine pollution, wherein Burkholderia HSY-3 or the above-mentioned bacterial agent is injected into the uranium mine seepage water or groundwater to be repaired at an inoculation rate of 1 to 10%, and a corresponding microbial culture system is simultaneously constructed. After the injection process is completed, the corresponding repair effect can be achieved under static conditions.

[0008] The present invention has the following beneficial effects:

[0009] The current lime neutralization technology is easily corroded and dissolved under the long-term natural environment, and the repair effect is unstable. Although the repair effect of reverse osmosis and other technologies is good, the cost is high. The microbial remediation technology provided by the present invention can significantly reduce the repair cost, while restoring the activity of the microbial community, providing long-term environmental stability and environmental resistance. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0011] Figure 1 :15d microbial agent growth status.

[0012] Figure 2 :15d microbial agent restoration effect. DETAILED DESCRIPTION

[0013] Now, various exemplary embodiments of the present invention are described in detail. The methods in the examples are conventional methods unless otherwise specified, and the reagents used are conventional commercially available reagents or reagents prepared by conventional methods unless otherwise specified. This detailed description should not be considered as a limitation of the present invention, but should be understood as a more detailed description of certain aspects, features and embodiments of the present invention.

[0014] It should be understood that the terms described in the present invention are only for describing a particular embodiment and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0015] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0016] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

[0017] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0018] Example 1 Microbial screening and culture system

[0019] The screening culture system components of Burkholderia HSY-3 in the present invention are: 3-3.5g of ferric citrate, 0.5-1g of ammonium chloride, 0.02-0.05g of calcium chloride, 0.3-0.5g of magnesium sulfate, 0.3-0.5g of dipotassium hydrogen phosphate, 0.1-0.2g of potassium dihydrogen phosphate, 5-10g of glucose, pH 6.8-7.2, and 1000mL of distilled water.

[0020] When the Burkholderia HSY-3 of the present invention is cultured in the culture system, the microorganism can grow stably at a temperature of 20 to 35° C. and under static conditions, and the preparation of the microbial agent can be completed after 3 to 5 days of culture.

[0021] Example 2 Bacteria domestication and cultivation

[0022] According to the main characteristic indicators of seepage water / groundwater in different uranium mines, especially in neutral / weakly acidic environments, pH value and U concentration were selected as the initial acclimation conditions. Taking the seepage water of a uranium mine in the south as an example, according to the main water quality characteristics of the seepage water, pH value is 6.0-7.5, U concentration is 0.5-2.0 mg / L, the functional microbial strains obtained by separation and screening were gradually acclimated to pH value and U concentration. For pH value, the pH value was set to 6.0 in the culture system. Under the condition of pH value 6.0, the number of viable cells of the microorganism reached 10 7 CFU / mL, take 1% of the bacterial agent and inoculate it into the next system. After the pH value is increased by 0.5 to 6.5, it is judged again that the number of viable cells of the microorganism reaches 10 7 CFU / mL, until the acclimation is completed in a pH 7.5 environment. At the same time, the U concentration also adopts this gradient acclimation method, with the initial uranium concentration of 0.5 mg / L gradually increasing from 0.5 mg / L to 2.0 mg / L. Finally, a microbial agent with tolerance to mine groundwater was obtained.

[0023] Example 3 In-situ repair

[0024] According to the pollution level, different microbial agents developed are injected into the uranium mine seepage water / groundwater to be repaired at an inoculation rate of 1-10%, and a corresponding microbial culture system is constructed at the same time. After the injection process is completed, the corresponding repair effect can be achieved under static conditions.

[0025] For the seepage water of uranium tailings reservoir of a hard rock uranium mine in southern my country, its water quality characteristics are pH value of 6.85 and uranium concentration of 0.5mg / L, which belongs to the general pollution level. Based on the water quality characteristics, when the microbial agent described in the present invention is used for in-situ remediation, the microbial agent obtained after domestication is inoculated into 10L of seepage water at a 1% inoculation amount, and the room temperature static remediation method is adopted. After 5 days of remediation at room temperature of 25-30℃, the number of microorganisms growing in the seepage water is significantly increased, the pH shows an upward trend, and the uranium concentration is reduced to the expected level. The results show that the microbial agent described in the present invention has significant tolerance and remediation ability for uranium mine seepage water.

[0026] Example 4 Results Analysis

[0027] 15d growth status and corresponding repair effect Figure 1 and Figure 2 As shown in the figure, it can be seen that the bacteria provided by the present invention have produced relatively significant results in the uranium pollution control method.

[0028] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A Burkholderia sp. strain HSY-3, characterized in that The strain was deposited in the General Microbiology Center of China Culture Collection Administration on January 26, 2024, with the address at No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, with the deposit number CGMCC NO.29789.

2. A bacterial agent, characterized in that The bacterial agent component includes the Burkholderia strain HSY-3 as claimed in claim 1.

3. Use of the Burkholderia strain HSY-3 as claimed in claim 1 in uranium mine pollution control.

4. Use of the bacterial agent as claimed in claim 2 in uranium mine pollution control.

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