An antibacterial peptide QLX-227-13, and a preparation method and application thereof

By designing an antimicrobial peptide QLX-227-13 composed of 14 amino acids, and using solid-phase synthesis and reversed-phase high-performance liquid chromatography purification, combined with D-type amino acid substitution, the problems of high production cost and low activity of existing antimicrobial peptides were solved, achieving safe and efficient antimicrobial effects, and making it suitable for a variety of antimicrobial products.

CN118406115BActive Publication Date: 2025-11-07SHANDONG UNIV QILU HOSPITAL
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Patent Information

Application Number
CN202410829412.X
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-03-20
Publication Date
2025-11-07
Estimated Expiration
2044-03-20

AI Technical Summary

Technical Problem

Existing technologies for the discovery and production of antimicrobial peptides suffer from problems such as long development cycles, high costs, low activity of natural antimicrobial peptides, easy degradation, high synthesis costs, and strong immunogenicity. Furthermore, the limited sequence arrangement of natural antimicrobial peptides makes large-scale production difficult.

Method used

An antimicrobial peptide QLX-227-13 composed of 14 amino acids was designed and synthesized by solid-phase synthesis and purified by reversed-phase high-performance liquid chromatography. By introducing D-type amino acid substitution and combining it with computer high-throughput screening, an antimicrobial peptide with specific properties was generated, which is suitable for the preparation of drugs and antimicrobial products against Gram-positive and Gram-negative bacteria.

Benefits of technology

The generated antimicrobial peptides have broad-spectrum antimicrobial activity, good safety, short peptide chains, and low synthesis cost. They are suitable for use in internal and external medicines, disinfectants, detergents, and feed additives, solving the problems of high cost and low activity in existing technologies and achieving safe and efficient antimicrobial effects.

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Abstract

The application belongs to the technical field of biology and specifically relates to an antibacterial peptide QLX-227-13 and a preparation method and application thereof. The amino acid sequence of the antibacterial peptide is shown as SEQ ID NO. 13. The antibacterial peptide of the application is derived from computer high-throughput screening, introduces preset functional attribute information, can generate antibacterial peptides with specific attributes, and is different from existing antibacterial peptides in nature, which makes up for the defects of limited arrangement of natural antibacterial peptide amino acid sequences. After strict detection and screening of antibacterial performance, hemolytic toxicity and cytotoxicity in vitro and in vivo, the antibacterial peptide has broad-spectrum antibacterial activity on gram-positive multi-drug resistant bacteria and / or gram-negative multi-drug resistant bacteria and good safety. The antibacterial peptide of the application is composed of 14 amino acids, the peptide chain is short, the preparation method and technology are mature, and the synthesis cost is low.
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