A method for cultivating wheat seedlings using green light

Abstract

The application belongs to the field of wheat planting, and particularly relates to a method for cultivating wheat seedlings by using green light. The method for cultivating wheat seedlings is used for Zhongke zinulai wheat 168, and the cultivation process is carried out under green light. The application provides a new specific method for cultivating wheat seedlings of Zhongke zinulai wheat 168, and the cultivation process is carried out under full green light. The specific Zhongke zinulai wheat 168 cultivated by the method can help the wheat seedlings to generate and accumulate more ASA (ascorbic acid), and has positive effects on improving the stress resistance of the wheat, promoting photosynthesis after transplanting, helping to bloom earlier, and improving the edible and commercial values of the wheat seedlings.

Description

TECHNICAL FIELD

[0001] The application belongs to the field of wheat planting, and particularly relates to a method for cultivating wheat seedling by using green light. BACKGROUND

[0002] Wheat seedling cultivation is an important stage for laying the whole growth and development state of the plant. Meanwhile, the wheat seedling contains rich nutrients such as dietary fiber, vitamins, trace elements and antioxidant active ingredients, and has the effects of removing free radicals in the body, resisting oxidation, resisting fatigue, regulating blood lipids, reducing blood pressure, improving gastrointestinal function and assisting weight loss. Therefore, with the continuous improvement of living standards, wheat seedling leaves are also developed into various foods, health products and even drugs.

[0003] In the cultivation of wheat seedling, light is one of the core cultivation conditions. Under different light conditions, the accumulation amount of various nutrients, metabolites and other substances closely related to growth and development in the wheat seedling obtained by wheat seedling cultivation is significantly different, and the apparent characteristics of the wheat seedling also appear obvious differences.

[0004] Generally speaking, red light has obvious promoting effect on the growth rate of wheat, and when the red light intensity is higher than that of blue light, red-blue composite light is more conducive to the growth of wheat. In addition, different monochromatic light has different effects on the phototropic growth of wheat coleoptile, and the bending degree of coleoptile under orange light is greater than that under blue-violet light. Under white light conditions, the bending degree of coleoptile is smaller than that under red, orange and purple light conditions.

[0005] However, with the continuous development of new wheat varieties, researchers have gradually found that the above rules are not universal, and the same light conditions may bring completely opposite results for different wheat varieties. This may be related to the genotype of different wheat varieties. Therefore, for varieties with different genetic backgrounds, it is necessary to re-explore the seedling cultivation conditions.

[0006] Zhongke Zinuo 168 is a spring wheat variety selected and bred from Zhongke Mai 138 and PW18, and has the disease resistance of medium resistance to stripe rust, high susceptibility to powdery mildew and medium susceptibility to sclerotinia disease. It not only has a unique purple whole waxy property, but also contains anthocyanins and other minerals. It is a new wheat variety bred by the Chengdu Institute of Biology, Chinese Academy of Sciences.

[0007] At present, the research on this wheat variety is still in the initial stage, and it is necessary to provide a specific seedling cultivation method for the variety. SUMMARY

[0008] The purpose of the present application is to provide a method for cultivating wheat seedling by using green light.

[0009] To achieve the above-mentioned object, the present application adopts the technical solution of a method for cultivating wheat seedlings, wherein the wheat is Zhongke Zinuo Wheat 168, and the cultivation of the wheat seedlings is performed under green light.

[0010] Preferably, after the wheat seeds start to germinate, the wheat seeds are placed under green light for the cultivation of the wheat seedlings.

[0011] Preferably, the method comprises the following steps:

[0012] (1) selecting full wheat seeds and soaking them until they start to germinate;

[0013] (2) selecting the seeds that have just started to germinate and have an intact structure, and growing them under green light, at a temperature of 20-28℃, and under alternating 16h green light illumination and 8h darkness for 10-16 days until the wheat seedlings reach the three-leaf stage.

[0014] Preferably, in step (1), the soaking is performed in 1%-1.5% hydrogen peroxide at a temperature of 4-10℃.

[0015] Preferably, the soaking in hydrogen peroxide is performed for 10-14h, and then the seeds are soaked in clean water until they start to germinate.

[0016] Correspondingly, a method for helping wheat seedlings to accumulate ascorbic acid, wherein the wheat is Zhongke Zinuo Wheat 168, and the cultivation of the wheat seedlings is performed under green light.

[0017] Preferably, the cultivation of the wheat seedlings is performed under alternating 16h green light illumination and 8h darkness.

[0018] Correspondingly, a method for increasing the dry leaf weight of wheat seedlings, wherein the wheat is Zhongke Zinuo Wheat 168, and the cultivation of the wheat seedlings is performed under green light.

[0019] Preferably, the cultivation of the wheat seedlings is performed under alternating 16h green light illumination and 8h darkness.

[0020] Correspondingly, a food, health care product or medicine prepared from the leaves of the wheat seedlings cultivated by the method, or a food, health care product or medicine containing the leaves of the wheat seedlings cultivated by the method.

[0021] The present application has the following beneficial effects: the present application provides a new specific seedling cultivation method for wheat 168, and the whole wheat seedling cultivation process is carried out under green light conditions. The specific wheat 168 variety wheat seedlings cultivated by the method of the present application can help the wheat seedlings to generate and accumulate more ASA (ascorbic acid, VC), which has a positive effect on improving the antioxidant capacity of wheat, reducing cell stress, and helping wheat to bloom earlier. At the same time, the wheat seedlings have the effect of improving the gastrointestinal function, and there are a large number of wheat seedling powder, wheat seedling juice and other health foods on the market. Ascorbic acid has various medical and health care effects, and increasing the content of ascorbic acid in wheat seedlings can effectively improve the edible and commercial value of wheat seedlings. BRIEF DESCRIPTION OF DRAWINGS

[0022] Figure 1 Fig. 1 is a schematic diagram of the wheat seedling cultivation of Zhongke Zinuo wheat 168 under green light treatment.

[0023] Figure 2 Fig. 2 is a schematic diagram of the wheat seedling cultivation of Zhongke wheat 138 under green light treatment. DETAILED DESCRIPTION

[0024] The present application provides a wheat seedling cultivation method. The wheat is Zhongke Zinuo wheat 168 (hereinafter referred to as wheat 168 in full), and the public can contact the Chengdu Institute of Biology of the Chinese Academy of Sciences to obtain wheat 168. The method comprises the following steps:

[0025] 1. Select plump wheat seeds, soak them in water for a period of time, preferably 12 h in 4-10℃, 1.2% (v / v) hydrogen peroxide, to help the seeds to break dormancy and enter the germination state.

[0026] 2. Select seeds that have started to turn white (i.e. germination) and are structurally complete, grow them under green light (wavelength 526 nm) at a temperature of 25℃, under light conditions of 16 h green light illumination + 8 h darkness, until the seedlings reach the three-leaf stage, under light intensity of 3000-5000 lux.

[0027] The technical solutions in the embodiments of the present application will be described clearly and completely in combination with the drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, not all the embodiments. If not specifically indicated, the technical means used in the embodiments are conventional means familiar to those skilled in the art. The obtained data are all average values obtained after at least 3 repetitions, and each repetition obtains valid data.

[0028] Example: Effect of different light treatments on wheat seedlings

[0029] 1. Selecting Zhongke Zinuaomai 168 (referred to as wheat 168) as the research material, and Zhongke Mai 138 (referred to as wheat 138) as the control material. Among them, wheat 168 is a purple whole waxy wheat variety, and wheat 138 is a high-yield wheat variety.

[0030] Selecting the full wheat seeds collected this year and naturally dried, and soaked in 1.2% hydrogen peroxide for 12 hours, and then replaced with water for 1-2 days, until the wheat seeds were in the initial germination state. Selecting the seeds of each variety that are in the initial germination state and have complete structure, and irradiating for 14 days at 25°C under different light treatments, with 10 replicates in each group.

[0031] The light treatments used in each group include: ultraviolet light (UV, 410 nm), blue light (B, 450 nm), sky blue light (T, 480 nm), green light (G, 526 nm), orange light (O, 590 nm), red light (R, 660 nm), far red light (Fr, 730 nm), full spectrum (Q), and darkness (D). Among them, except for darkness (D) which means 24h continuous darkness treatment, the rest of the light treatments mean corresponding light treatment for 16h and darkness treatment for 8h, alternating, with light intensity of 5000lux. For example: UV treatment means UV treatment for 16h and darkness treatment for 8h, alternating. After the treatment of each group is completed, the seedling phenotypes are investigated, and the ASA (ascorbic acid) content in the leaves is measured.

[0032] 2. Using the Solabio ascorbic acid (ASA) content detection kit (BC1230-50T / 48S) kit to determine ASA. Take 0.1g of fresh wheat leaves from each group, add 1mL of kit extraction solution, and homogenize in an ice bath. Then centrifuge at 8000g and 4°C for 20min, take the supernatant and place it on ice. Prepare standard tubes and measurement tubes, and use an enzyme marker to measure (set wavelength 265nm), record the absorbance values at 10s and 130s, standard tube absorbance is recorded as A1 and A2, ΔA standard tube = A1-A2, measurement tube records the absorbance of the sample at 10s and 130s, recorded as A3 and A4, ΔA measurement tube = A3-A4. According to the formula ASA content (nmol / g mass) = 400 x ΔA measurement tube ÷ ΔA control tube ÷ W, where W is the sample mass, the mass of ASA is obtained. The results are shown in Table 1. Unit: nmol / g.

[0033] Table 1: Comparison of ASA results in each group

[0034]

[0035] The results showed that wheat 168 exhibited different traits and light properties from conventional wheat. If no light quality screening is performed, the light treatment condition used in the breeding process is more likely to be full spectrum covering all wavelengths, which is considered suitable for the entire growth and development process. However, under full spectrum conditions, the ASA content in wheat 168 is extremely low, only 297.8 nmol / g, which is about 5% of that in conventional wheat 138. While under dark conditions, the ASA content of wheat 138 decreased slightly from 6573.6 nmol / g to 5850 nmol / g, indicating that full spectrum light has no significant effect on ASA synthesis in conventional wheat. However, in wheat 168, ASA increased to 1451 nmol / g under dark conditions, indicating that full spectrum light conditions seem to inhibit the synthesis of ASA in wheat 168. Therefore, if high ASA content wheat seedlings are screened under commonly used full spectrum and natural light conditions close to full spectrum, wheat 168 will be eliminated according to the general screening approach.

[0036] In addition to full spectrum treatment, monochromatic light irradiation is also a common light treatment method. Currently, the commonly used monochromatic light is blue light and red light. Among them, blue light is commonly used for seedling cultivation, while red light is mostly used for inducing fruit coloration and ripening stage. Compared with red and blue light, the effect of other visible light on wheat growth and development has been less studied. Ultraviolet light, which is outside the visible light range, has been found to be related to the accumulation of anthocyanin content in wheat grains. Therefore, in addition to red and blue light, this study also adopted green and orange light irradiation within the visible light range, and added ultraviolet and far infrared light irradiation outside the visible light range, to observe whether the effect of different wavelengths of light sources on ASA is linear, hoping to explain the inhibitory effect of full spectrum on wheat 168.

[0037] The results showed that:

[0038] (1) Contrary to the guess, for both wheat 138 and wheat 168, the wavelength did not show a linear relationship with the accumulation of wheat seedling ASA. This indicates that the effect of different light treatments on ASA accumulation cannot be predicted by the length of the wavelength.

[0039] (2) For wheat 138, taking the ASA content under full spectrum as the basic control, the effect of different wavelengths of light sources on wheat ASA content can be divided into the promotion group: UV and B (both groups have significantly higher ASA than the full spectrum condition), the inhibition group: G and R (both groups have significantly lower ASA than the full spectrum condition), and the insensitive group: Fr and O (both groups have similar ASA to the full spectrum condition). For wheat 168, all treatments were significantly higher than the ASA content under full spectrum conditions. This indicates that different wheat varieties do not have a predetermined pattern for different light treatments, and only the specific treatment can be used to screen the appropriate conditions.

[0040] (3) Under the blue light condition commonly used for seedling raising, the ASA content of both wheat 138 and wheat 168 was significantly improved, in which the ASA content of wheat 138 was improved by 2.66 times compared with that under the full spectrum condition, and the ASA content of wheat 168 was improved by 47.77 times, but was still slightly lower than that of wheat 138. It is shown that under the blue light condition, the ASA content of different wheat materials can be improved, but the improvement range, i.e. the sensitivity, is different, and the improvement range of wheat 168 is higher, but since the ASA background level of wheat 168 under the full spectrum condition, i.e. the conventional condition, is extremely low, the ASA of wheat 168 is still lower than that of wheat 138 after the blue light treatment, and if only the common blue light treatment is used, the ASA improvement potential of wheat 168 will not be found.

[0041] (4) Compared with the full light and dark treatment, the ASA accumulation of wheat 138 under the green light treatment is greatly reduced, which is only about 1 / 3 of the full light treatment and only about 1 / 2 of the dark treatment, proving that the conventional wheat is not accumulated with ASA when the wheat seedling is cultivated with the full green light. However, wheat 168 shows completely opposite results, and the ASA value accumulated in the wheat seedling under the green light treatment is 10.67 times of that of wheat 138 and 103.12 times of that under the full spectrum condition, and is significantly higher than that under other light conditions, even more than 2 times of that under the blue light treatment, which proves that the green light treatment can help wheat 168 to generate and accumulate ASA with high efficiency.

[0042] The green light treatment shows completely different results on wheat 168 and wheat 138, which proves that the light treatment effect is closely related to the specific variety of wheat and the physiological and biochemical performance of the specific variety. After changing the variety, the green light treatment may not have a positive promoting effect or even have a negative effect.

[0043] 3. Further comparison of the seedling length and leaf weight of each wheat under the green light treatment. The seedling period of wheat 168 of Tsinghua purple glutinous wheat is as shown in Figure 1 , and the seedling period of wheat 138 of Tsinghua wheat is as shown in Figure 2 . The results are shown in Table 2. In Table 2, the loss = fresh leaf weight - dry leaf weight.

[0044] Table 2: Comparison table of seedling phenotype results under green light treatment of each group

[0045]

[0046] The results show that: under the full spectrum condition, the seedling height and fresh leaf weight of wheat 168 and wheat 138 have no significant difference, that is, under the normal light condition, the biomass of wheat 168 and wheat 138 has no significant difference at the seedling stage, and wheat 138 can be used as a control to analyze the change of ASA content in wheat 168. Under green light treatment, the seedling height of wheat 168 is significantly higher than that of wheat 138, indicating that green light can promote the growth of wheat 168 seedlings, but the fresh leaf weight is significantly lower than that of wheat 138, suggesting that it may be more "tall and thin", resulting in a decrease in fresh weight. Moreover, under green light conditions, the fresh leaf weight of wheat 138 is significantly increased compared with itself under full spectrum conditions, while the fresh weight of wheat 168 changes little. But unexpectedly, the dry leaf weight of wheat 168 is significantly higher than that of wheat 138, even higher than that of itself under full spectrum conditions, while wheat 138 is on the contrary, the fresh leaf weight increases under green light conditions, but the dry leaf weight is lower than that under full spectrum conditions; It shows that the dry matter accumulation of wheat 168 under green light conditions is actually higher. Further analysis of the loss shows that the loss of wheat 168 during drying is significantly lower than that of wheat 138, which may be related to the difference in water content, etc.

[0047] In commercial factory processing of wheat seedlings, it is generally necessary to dry the seedlings first to obtain dry powder, and then to proceed to the next step. Therefore, under green light conditions, the dry matter content of wheat 168 accumulates more, the dry leaf weight is higher, the output is higher, and it is more beneficial to prepare food and medicine related to wheat seedlings, as well as the extraction and utilization of ASA and other substances.

[0048] The above-described embodiments are only to describe the preferred modes of the present application, and not to limit the scope of the present application, and various modifications, variations, modifications, replacements of the technical solutions of the present application made by those skilled in the art without departing from the design spirit of the present application shall fall within the protection scope determined by the claims of the present application.

Claims

1. A method of assisting in the accumulation of ascorbic acid in wheat seedlings, characterized by: The wheat is Zhongke zinuo wheat 168, and the wheat seeds are placed under green light for wheat seedling cultivation after the wheat seeds start to germinate.

2. The method of claim 1, wherein: The method comprises the following steps: (1) selecting full wheat seeds, soaking to the starting germination state; (2) selecting the seeds starting to germinate and being complete in structure, growing under the alternating treatment of 16 h green light illumination and 8 h darkness at 20-28 DEG C for 10-16 days, to the three-leaf stage of the wheat seedling.

3. The method of claim 2, wherein: In step (1), soaking in 4-10 DEG C, 1%-1.5% hydrogen peroxide.

4. The method of claim 3, wherein: Soaking in the hydrogen peroxide for 10-14 h, and then soaking in clean water to the starting germination state.

5. The method of claim 1, wherein: Cultivating the wheat seedling under the alternating treatment of 16 h green light illumination and 8 h darkness.

6. Food, health care product or medicine prepared from the wheat seedling leaves cultivated by the method according to any one of claims 1-5, or food, health care product or medicine containing the wheat seedling leaves cultivated by the method according to any one of claims 1-5.

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