An external skin-regenerating oil preparation for turning white hair into black hair without bleaching or dyeing, and its preparation method and application

Abstract

The present invention relates to the technical field of new drug indications, and specifically discloses an external skin-rejuvenating oil preparation for turning white hair into black hair without bleaching and dyeing, and its preparation method and application. The preparation method of the external skin-rejuvenating oil preparation is as follows: 1) Preparation of freeze-dried powder of skin-rejuvenating oil alcohol extract: taking raw materials such as astragalus, angelica, tangerine peel, rhubarb, scutellaria, coptis root, phellodendron, borneol, etc. according to a certain weight ratio, and adding them to a container respectively, and then adding an ethanol solution with a volume fraction of 50%-90% to the container, soaking, flash extraction, filtrate concentration, freeze drying to obtain; 2) dissolving the freeze-dried powder of the skin-rejuvenating oil alcohol extract and egg yolk lecithin in vegetable oil to prepare an oil phase, and then dropping the oil phase into the water phase obtained by dissolving Tween 60 and glycerol in ultrapure water, stirring and high-speed shearing, high-pressure homogenization, to obtain. The external skin-rejuvenating oil preparation of the present invention has the effect of quickly nourishing the scalp, increasing the elasticity and toughness of the hair, and realizing the effect of turning white hair into black in a short period of time.

Description

Technical Field

[0001] The invention relates to the technical field of new drug indications, and in particular to an external skin-regenerating oil preparation for converting white hair into black hair without bleaching or dyeing, and a preparation method and application thereof. Technical Background

[0002] Black hair not only represents a kind of natural beauty, but also a manifestation of youth and health. But apart from genetic factors, with age and health changes, hair begins to fade and even gradually turns white (gray hair). People with gray hair, especially those with gray hair at a young age, usually use bleaching agents to artificially dye their hair to maintain their personal good image. However, the long-term use of chemical hair dyes (mostly amine or phenolic oxidative hair dyes) will not only destroy the scalp's protective film, but is also more likely to cause allergies, inflammation, scalp cancer and other diseases. Therefore, finding a drug that can quickly turn gray hair black and is safe has become the pursuit of gray hair researchers and the expectation of gray hair people around the world.

[0003] White hair refers to a skin disease in which part or all of the hair turns white, which is a loss of pigment or disappearance. It can be divided into congenital and acquired types. Congenital white hair has a family history, and localized white hair at the hairline is more common. Compared with the difficult-to-treat congenital white hair, acquired white hair can be divided into juvenile white hair (with a family history, and no other physical health problems except for the increase in white hair) and senile white hair, and there are many treatment options (including improving nutrition and adjusting mental state, as well as treating corresponding diseases).

[0004] The reason why black hair turns white is nothing more than mental factors, autoimmunity, genetic factors and aging, which lead to a lack of melanin that forms black hair (microscopically, the number of pigment cells decreases, the melanin function of melanosomes decreases, or the number of melanosomes decreases; in fact, nearly 80% of the population does not have the problem of a decrease in the number of pigment cells), or insufficient nutrition supplied to the scalp and hair (lack of pigment materials or the ability to transport pigment materials is blocked; it can be understood as a microcirculation disorder that transports nutrients and oxygen to the scalp and hair). Studies have shown that mental tension and stress can cause melanin stem cells in the hair to migrate to the skin. The greater the stress, the more melanin stem cells migrate, which causes the hair to turn white and the skin pigmentation to increase. Therefore, people who are under great stress and often stay up late are prone to white hair and a dull complexion. In addition, mental tension and excessive stress can increase the level of adrenaline hormones in the body, causing contraction of the peripheral blood vessels that supply nutrition to the hair, and subsequently impairing the melanin-producing function of the hair papilla at the root of the hair (even if melanin can still be produced, the output channel is blocked), resulting in a large amount of gray hair in a short period of time.

[0005] At present, there is no effective drug in Western medicine for the treatment of gray hair. In the long-term clinical practice, Chinese medicine has accumulated a large number of Chinese medicines and Chinese medicine prescriptions for black hair. For example, Volume 6 of Shoushibaoyuan records Chinese medicines such as He Shouwu, Eclipta prostrata, Sophora japonica, and Rehmannia root, which have the effects of tonifying the kidney and clearing heat, strengthening teeth and improving eyesight, blackening hair and beard, and moisturizing the mouth and body. In addition, the long-term use of prescriptions such as Shouwu tablets and Liuwei Dihuang pills can help turn gray hair black. Based on the Chinese prescription database, a search was conducted and it was found that Chinese medicine believes that the growth of hair is most closely related to the liver and kidney (such as the kidney stores essence and the kidney is in the hair; the liver stores blood and the hair is the rest of the blood, etc.). Nearly 700 groups of prescriptions related to nourishing the kidney and blood, clearing dryness and heat, and relieving liver depression can also be found, which have very important guiding value for the clinical treatment of gray hair. However, it is necessary to remember that some Chinese medicines such as He Shouwu have clear toxicity and cannot be taken for a long time or in large quantities. In addition, traditional Chinese medicine's internal use of Chinese medicine for blackening hair takes a long time and has limited effects.

[0006] In ancient times, doctors also mentioned that "when blood is abundant, hair is moist, and when blood is weak, hair is weak" (Ming Dynasty doctor Li Zhen in "Introduction to Medicine") and "when blood is abundant, hair is prosperous, and hair is beautiful..." (Wang Kentang in "Standards for Diagnosis and Treatment"), pointing out that there is a close relationship between hair and pathological changes such as the prosperity and decline of blood and qi and stasis. However, traditional Chinese medicine for external use of black hair is more frequently used with natural plant dyes represented by gallnut, terminalia chebula, and saponin, and natural minerals represented by alum and red copper, and it is clear that the main purpose of external use of black hair is dyeing hair. In other words, traditional Chinese medicine for external use of black hair is not the non-bleaching and dyeing type of white hair turning black advocated by the inventor.

[0007] It is obvious that in the actual process of turning gray hair into black hair, Chinese medicine practitioners do not focus on using Chinese medicine such as "replenishing qi and nourishing blood, promoting blood circulation and removing blood stasis", but instead focus on "nourishing the liver and nourishing the kidneys". Therefore, whether it is taken internally or applied externally, the effect expected by people with gray hair is not achieved. Summary of the invention

[0008] In view of the problem that there is a lack of non-bleaching and dyeing external-use special-effect drugs for turning white hair into black hair in the above-mentioned prior art:

[0009] The first purpose of the present invention is to provide a non-bleaching and dyeing external skin-regenerating oil preparation that can turn gray hair into black in a short period of time. The external skin-regenerating oil preparation is obtained by the inventor after substantial adjustments to the early external skin-regenerating oil formula of the inventor's team for the treatment of gray hair.

[0010] The second object of the present invention is to provide a method for preparing the above-mentioned external skin-regenerating oil preparation for treating white hair.

[0011] The third object of the present invention is to provide the medical use of the above-mentioned external skin-regenerating oil preparation for treating white hair.

[0012] The inventive concept of the present invention is as follows:

[0013] Gray hair is an obvious sign of gradual aging, but aging-related gray hair itself does not have a significant impact on human life activities. In order to reduce or even prevent gray hair from revealing the message of "aging" to others, people with gray hair usually choose hair dyeing that is simple to operate and has a quick effect. Unfortunately, after dyeing with chemical hair dyes, the white hair roots will appear in about a month. In order to cover up gray hair, you have to continue dyeing your hair every month, even though everyone knows that hair dyes are very damaging to the hair and scalp. Because bleaching and dyeing gray hair to black hair poses certain health risks to the body, it is very necessary to develop external drugs that can turn non-bleached and dyed gray hair to black hair.

[0014] Based on the pathological characteristics of gray hair, the applicant believes that gray hair is mainly caused by the obstruction of local meridians in the scalp, which leads to stagnation of qi and blood, causing the hair to lose its nutrition and gradually fade. Therefore, the applicant pointed out that in the treatment of gray hair, priority should be given to the possibility that the human scalp and hair still have difficulty obtaining the nutrients that form melanin under living conditions with very rich nutrients. The applicant adopts the prescription "Shengfu Oil" that "replenishes qi and nourishes blood, activates blood circulation and removes blood stasis", and uses a non-bleaching and dyeing mode to achieve the effect of quickly turning gray hair black.

[0015] In order to achieve the first purpose, the present invention adopts the following technical measures:

[0016] A topical skin-rejuvenating oil preparation for treating white hair is prepared from the following raw materials in proportions by weight: 5-30 parts by weight of astragalus, 5-30 parts by weight of angelica, 5-30 parts by weight of tangerine peel, 5-30 parts by weight of scutellaria, 5-30 parts by weight of coptis root, 5-30 parts by weight of phellodendron, 5-30 parts by weight of rhubarb, and 3-5 parts by weight of borneol.

[0017] In order to achieve the second purpose, the present invention adopts the following technical measures:

[0018] A method for preparing the above-mentioned external skin-regenerating oil preparation for treating gray hair, the steps of which are as follows: The present invention provides a skin-regenerating oil preparation, which is a water-in-oil preparation of skin-regenerating oil alcohol extract, and the solute of the skin-regenerating oil preparation is a freeze-dried powder of skin-regenerating oil alcohol extract; wherein the content of skin-regenerating oil alcohol extract in the water-in-oil preparation of skin-regenerating oil alcohol extract is greater than 0 and not more than 30 mg / ml, preferably the content is not less than 10 mg / ml and not more than 30 mg / ml, and more preferably the content is 30 mg / ml.

[0019] Among them, the preparation method of the lyophilized powder of the alcohol extract of raw skin oil is as follows: 8 raw materials of raw skin oil (the weight of each raw material is: 5-30 weight parts of astragalus, 5-30 weight parts of angelica, 5-30 weight parts of tangerine peel, 5-30 weight parts of scutellaria, 5-30 weight parts of coptis, 5-30 weight parts of phellodendron, 5-30 weight parts of rhubarb, 3-5 weight parts of borneol) are taken in a certain weight ratio, a total of 500g, except for borneol, the remaining drugs are ground (passed through a 200 mesh sieve) and placed in a 5L stainless steel cup, 2.9L-4.4L of ethanol with a volume fraction of 50%-90% is added, soaked for 2-3h, flash extracted 3-5 times, each time for 5min, and the filtered supernatant is quickly concentrated by a multi-stage flash evaporator, and then freeze-dried to obtain the alcohol extract of raw skin oil (the yield is 11.7%-13.6%).

[0020] Next, the present invention also provides a method for preparing a skin-enhancing oil preparation using a freeze-dried powder of a skin-enhancing oil alcohol extract, the method comprising the following steps: dissolving the freeze-dried powder of the skin-enhancing oil alcohol extract and egg yolk lecithin in an edible vegetable oil to prepare an oil phase, and dripping the oil phase into an aqueous phase prepared by dissolving Tween 60 and glycerol in ultrapure water at a volume ratio of 1:4 between the oil phase and the aqueous phase, stirring, high-speed shearing, and high-pressure homogenization to obtain; the zeta potential and particle size distribution of the obtained skin-enhancing oil-in-water preparation of the alcohol extract are uniform; its particle size distribution is 141 to 825 nm, and there is no multiple scattering phenomenon, the average particle size is 290±10 nm; the average zeta potential is -50 mV to -40 mV. Wherein, the edible vegetable oil is selected from at least one of sesame seed oil and olive oil. Preferably, the method comprises the following steps:

[0021] 1) Dissolving the freeze-dried powder of the alcohol extract of raw skin oil and egg yolk lecithin in edible vegetable oil to prepare an oil phase; the final concentration of the egg yolk lecithin is 6% (W / V; g / ml); 2) Dissolving Tween 60 and glycerol in ultrapure water to prepare an aqueous phase; the final concentration of Tween 60 is 1.2% (V / V); the final concentration of glycerol is 2% (V / V); 3) Under the condition of a constant temperature water bath at 60° C., the oil phase prepared in step 1) is dripped into the aqueous phase prepared in step 2), and stirred at 1200 r / min for 15 min; the volume ratio of the oil phase to the aqueous phase is 1:4; 4) High-speed shearing is performed at 10000 rpm for 2 min, and then high-pressure homogenization is performed at 1000 bar for 6 times to obtain an oil-in-water preparation of the alcohol extract of raw skin oil.

[0022] In order to achieve the third purpose of the present invention, the present invention also adopts the following technical measures:

[0023] The use of the above-mentioned external skin-regenerating oil preparation or the external skin-regenerating oil preparation prepared by the above-mentioned method in the preparation of a drug for preventing or treating gray hair, especially in the preparation of a drug for preventing or treating non-bleached and dyed gray hair from turning black hair. Further, the gray hair is acquired gray hair, including juvenile gray hair and senile gray hair.

[0024] The formulation principles of each Chinese medicinal raw material of the present invention are as follows:

[0025] Astragalus, Chinese angelica, dried orange peel, etc. are the main ingredients that nourish qi and blood; rhubarb removes blood stasis and dredges meridians, which is the minister; scutellaria, coptis, phellodendron, etc. (TCM believes that blood heat and wind dryness can easily damage yin blood, and boiling blood will cause blood stasis, so this prescription uses heat-clearing and blood-cooling products to prevent blood stasis) cool blood and eliminate evil, which is the auxiliary; borneol and vegetable oil (use their viscosity to cover the scalp and hair, keep the hair hydrated in the form of an oil film, and prevent the hair from drying out) are the envoys. All the medicines work together to improve the microcirculation of the scalp, so that nutrients and oxygen can reach the hair root cells, which can not only nourish yin and moisturize dryness, maintain the elasticity and toughness of hair, but also activate the hair root cells to secrete melanin particles, and finally turn white hair black without bleaching or dyeing.

[0026] Compared with the prior art, the present invention has the following advantages and effects:

[0027] The research results show that the skin-regenerating oil preparation prepared by the present invention can quickly nourish the scalp and hair, increase the elasticity and toughness of the hair, and increase the number of white hair turning black and the thickness of black hair within a few days; and in just one month, almost all white hair is transformed into gray hair (mostly black hair); and during the application period, there is no abnormal stimulation and discomfort such as hair loss and dandruff.

[0028] The external skin-regenerating oil preparation of the present invention has the following advantages for turning gray hair into black hair: 1. The drug cost is low, it is non-irritating, has no toxic side effects, and has no health threats to the body caused by other bleaching and dyeing hair dyes; 2. The operation is extremely simple (only a thin layer is applied to the scalp and hair roots, and there is no need to apply it to the entire hair. For example, 2 to 3 ml of the skin-regenerating oil preparation is dripped on the scalp and hair roots at the temples and the top of the head, and quickly spread with a comb), and the topical administration directly reaches the scalp and hair roots; 3. For people with gray hair of different ages, it has a good effect on promoting recovery, and the time required is very short. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 The particle size and potential of the skin-regenerating oil preparations prepared in Examples 1 and 2 are characterized in FIG.

[0030] Figure 2 This is a graph showing the effect of the skin-regenerating oil preparation prepared in Example 1 on the transformation of white hair to black.

[0031] Figure 3 This is a graph showing the effect of the skin-regenerating oil preparation prepared in Example 1 on the diameters of white hair and black hair.

[0032] Figure 4 This is a graph showing the effect of the skin-regenerating oil preparation prepared in Example 2 on the transformation of white hair to black.

[0033] Figure 5 This is a graph showing that the skin-regenerating oil preparation prepared in Example 1 has no effect on the blackening of white hair in vitro. DETAILED DESCRIPTION

[0034] The applicant will further explain the technical solution of the present invention in detail below in conjunction with the accompanying drawings and specific embodiments. It should be understood that the following content should not limit the protection scope of the present invention in any way.

[0035] The dry powders of the seven Chinese medicinal materials, namely, astragalus, angelica, dried orange peel, rhubarb, scutellaria, coptis and phellodendron, used in the embodiments of the present invention were all sieved through a 200-mesh sieve.

[0036] Example 1: Preparation and characterization of skin-regenerating oil preparation

[0037] 1) Preparation of skin-regenerating oil preparation

[0038] Preparation of freeze-dried powder of raw skin oil alcohol extract: Take 343g of raw skin oil raw materials (80g each of dry powder of astragalus, angelica, and tangerine peel, 30g of dry powder of rhubarb, 20g each of dry powder of scutellaria, coptis, and phellodendron, and 13g of borneol), stir evenly and place in a 5L stainless steel cup, add 2L of 90v / v% ethanol, soak for 3h, flash extract 3 times, each time for 5min, and quickly concentrate the supernatant filtered three times by a multi-stage flash evaporator, and then freeze-dry to obtain freeze-dried powder of raw skin oil alcohol extract (40.1g).

[0039] Preparation of skin-generating oil preparation: ① Dissolve the freeze-dried powder of skin-generating oil alcohol extract and egg yolk lecithin in sesame seed oil to prepare the oil phase, and the final concentration of egg yolk lecithin is 6% (W / V). ② Dissolve Tween 60 and glycerol in ultrapure water to prepare the water phase, and the final concentration of Tween 60 is 1.2% (V / V), and the final concentration of glycerol is 2% (V / V). ③ Under the condition of a 60°C constant temperature water bath, drop the oil phase into the water phase, stir at 1200r / min for 15min, and the volume ratio of the oil phase to the water phase is 1:4. ④ High-speed shearing at 10000rpm for 2min, and then high-pressure homogenization at 1000bar for 6 times to obtain a skin-generating oil alcohol extract water-in-oil preparation, referred to as a skin-generating oil preparation, and the content of the skin-generating oil alcohol extract in the obtained skin-generating oil preparation is equal to 30mg / ml.

[0040] 2) Characterization of particle size and zeta potential of skin-regenerating oil preparations

[0041] When measuring the particle size of the skin-regenerating oil preparation, the sample was diluted with ultrapure water to make its count rate between 200 and 300 Kcps (1,000 counts per second), and then the particle size and polydispersity index were measured with a laser particle size analyzer at a scattering angle of 90° and a temperature of 25°C. When measuring the zeta potential, the conductivity of the skin-regenerating oil preparation was adjusted to 50 S / cm with a 0.9% (w / w) sodium chloride solution. Figure 1 As shown in (A, B), the particle size of the skin-regenerating oil preparation prepared in Example 1 is mainly distributed in the range of 141 to 531 nm, the average particle size is about 290 nm, and there is no multiple scattering phenomenon; and the zeta potential is evenly distributed, with an average of -42 mV.

[0042] Example 2: Preparation and characterization of skin-regenerating oil preparation

[0043] 1) Preparation of skin-regenerating oil preparation

[0044] Preparation of freeze-dried powder of raw skin oil alcohol extract: Take 228g of raw skin oil raw materials (30g each of dry powder of astragalus, angelica, tangerine peel, scutellaria, rhubarb, coptis, and phellodendron, 18g of borneol), stir evenly and place in a 5L stainless steel cup, add 2L of 50v / v% ethanol, soak for 3h, flash extract 5 times, 5min each time, and quickly concentrate the supernatant filtered five times by a multi-stage flash evaporator, and then freeze-dry to obtain freeze-dried powder of raw skin oil alcohol extract (31g).

[0045] Preparation of skin-generating oil preparation: ① Dissolve the freeze-dried powder of skin-generating oil alcohol extract and egg yolk lecithin in olive oil to prepare the oil phase, and the final concentration of egg yolk lecithin is 6% (W / V). ② Dissolve Tween 60 and glycerol in ultrapure water to prepare the water phase, and the final concentration of Tween 60 is 1.2% (V / V), and the final concentration of glycerol is 2% (V / V). ③ Under the condition of a 60°C constant temperature water bath, drop the oil phase into the water phase, stir at 1200r / min for 15min, and the volume ratio of the oil phase to the water phase is 1:4. ④ High-speed shearing at 10000rpm for 2min, and then high-pressure homogenization at 1000bar for 6 times to obtain a skin-generating oil alcohol extract water-in-oil preparation, referred to as a skin-generating oil preparation, and the content of the skin-generating oil alcohol extract in the obtained skin-generating oil preparation is equal to 30mg / ml.

[0046] 2) Characterization of particle size and zeta potential of skin-regenerating oil preparations

[0047] When measuring the particle size of the skin-regenerating oil preparation, the sample was diluted with ultrapure water to make its count rate between 200 and 300 Kcps (1,000 counts per second), and then the particle size and polydispersity index were measured with a laser particle size analyzer at a scattering angle of 90° and a temperature of 25°C. When measuring the zeta potential, the conductivity of the skin-regenerating oil solution was adjusted to 50 S / cm with a 0.9% (w / w) sodium chloride solution. Figure 1 As shown in (C, D), the particle size of the skin-regenerating oil preparation prepared in Example 2 is mainly distributed in the range of 142 to 825 nm, the average particle size is about 295 nm, and there is no multiple scattering phenomenon; and its zeta potential is evenly distributed, with an average of -47.3 mV.

[0048] Example 3 Effect of the skin-regenerating oil preparation prepared in Example 1 on the transformation of acquired white hair to black

[0049] Volunteer Professor T1, 54 years old, is in good health and has no other skin diseases except gray hair. During Professor T1's early years studying and working abroad (1998-2009), his hair gradually faded and was almost completely white by the end of 2008. From 2009 to 2023, he used Rishiri Kelp Hair Dye and CIELO Hair Dye irregularly, but the effect lasted less than a month after application. At the end of February 2024, he used a skin-regenerating oil preparation for external application.

[0050] Before the evaluation, go to the barber shop to cut the hair on the temples and the top of the head short, then wash and dry it and take a clear photo. Then add 2-3 ml of the skin-regenerating oil preparation prepared in Example 1 to the scalp and hair roots on the temples and the top of the head, and spread it quickly with a comb (a small amount of the skin-regenerating oil preparation may be transferred to the hair); once a day, keep it for at least 1 hour each time, and then wash it. During the application period, do not deliberately change your personal eating habits and work and rest habits. In order to intuitively see the repair effect of the skin-regenerating oil preparation, volunteer Professor T1 used a mobile phone to take high-definition photos before and after application for comparative analysis of the changes from white hair to black.

[0051] The results are as follows Figure 2As shown in the figure, the upper part (left) and the lower part (right) show that from before the application of the skin-regenerating oil preparation to 30 days after continuous application (in order to identify the number of hairs with the naked eye, I went to the barber shop and trimmed my temples appropriately), the sideburns: (1) turned from white to black overall; (2) using the special software of the microscope to count, taking the yellow dotted circle in the picture as an example, before the application, the number of black hairs on the left was 43, and after 4 days, 10 days, and 30 days of application, the number of black hairs was 118, 159, and 193 respectively; before the application, the number of black hairs on the right was 50, and after 30 days of application, the number of black hairs increased to 150. This shows that the degree of white hair turning black (measured by the number of black hairs on the same scalp area in the same area) has increased significantly; (3) Figure 3 As shown in the figure, compared with the surrounding white hair (randomly select 3 white hairs on the top of the head and take the part one centimeter away from the scalp, the average diameter is 87.3 microns; the length of the ruler in the picture is 100 microns), the thickness of the black hair (take the 3 black hairs next to the front 3 white hairs and measure the diameter of the part one centimeter away from the scalp, the average value is 101.4 microns; the length of the ruler in the picture is 100 microns) is significantly increased (this part of the test is completed by using the software of the scanning system after Z-axis scanning using the IX71 inverted microscope of Nikon Company of Japan); (4) compared with the surrounding white hair, by touching, it can be felt that the black hair is more elastic and tough; (5) There is no special color change or dandruff on the scalp.

[0052] Example 4 Effect of the skin-regenerating oil preparation prepared in Example 2 on the transformation of acquired white hair to black

[0053] Volunteer Professor T2, 62 years old, is in good health and has no other skin diseases except gray hair. In the second year of Professor T2's work at Huazhong University of Science and Technology (formerly Huazhong University of Science and Technology) in his early years (1988-1991), his hair was almost completely white. Since then, he has used hair dye after getting a haircut at the barber shop from time to time, but the effect is not ideal; in recent years, he refused to use bleaching and dyeing products to blacken his hair, and instead used products such as Codonopsis pilosula, deer antler, and wolfberry, but he hardly saw the desired effect. In mid-March 2024, he used a skin-regenerating oil preparation for external application.

[0054] Before the evaluation, go to the barber shop to cut the hair on the temples and the top of the head short, then wash and dry it and take a clear photo. Then add 2-3 ml of the skin-regenerating oil preparation prepared in Example 2 to the scalp and hair roots on the temples and the top of the head, and spread it quickly with a comb (a small amount of the skin-regenerating oil preparation may be transferred to the hair); once a day, keep it for at least 1 hour each time, and then wash it. During the application period, do not deliberately change your personal eating habits and work and rest habits. In order to intuitively see the repair effect of the skin-regenerating oil preparation, volunteer Professor T2 used a mobile phone to take high-definition photos before and after the application for comparative analysis of the changes from white hair to black.

[0055] The results are as follows Figure 4 As shown in the figure, the upper half (left) and the lower half (right) indicate that from before application of the skin-regenerating oil preparation to 10 days of continuous application, the sideburns: (1) changed from white to black overall, and according to Professor T2 himself, the hair growth rate was faster than before; (2) using the microscope's special software for counting, taking the yellow dotted circle in the picture as an example, before application, the number of black hairs on the left was 96, and after 1 day, 4 days, and 10 days of application, the number of black hairs was 121, 139, and 182 respectively; before application, the number of black hairs on the right was 99, and after 1 day, 4 days, and 10 days of application, the number of black hairs was 111, 201, and 214 respectively. This indicates that the degree of white hair turning to black (measured by the number of black hair on the same scalp area in the same region) has increased significantly; (3) the thickness of the black hair has increased significantly compared with the surrounding white hair; (4) by touching, the black hair can be felt to have greater elasticity and toughness than the surrounding white hair; (5) there is no special color change or dandruff on the scalp.

[0056] Example 5 The skin-regenerating oil preparation prepared in Example 1 has no effect on the blackening of isolated white hair

[0057] The above two application examples have clearly shown that the skin-regenerating oil preparation can quickly and effectively promote the transformation of white hair to black. So, can the skin-regenerating oil preparation promote the transformation of white hair to black and belong to the bleaching and dyeing category of hair dyes like the black hair materials circulating in the market? In order to answer this mystery, the inventor took the white hair on the top of the head of volunteer Professor T1 (19 white hair samples were taken before the first application of the skin-regenerating oil preparation), took a photo, washed it with Clear shampoo, and then placed it in a beaker (containing the skin-regenerating oil preparation prepared in Example 1) so that the white hair was completely immersed under the liquid surface, and the entire beaker was sealed with plastic wrap, and then left to stand in a 37°C thermostat for 48 hours (the temperature and time were selected to simulate the scalp temperature in Examples 3 and 4 and the total time that the scalp and hair were in contact with the skin-regenerating oil), took it out after soaking for 48 hours, washed it with Clear shampoo, and took a photo. In order to visually see whether the skin-growing oil preparation has the ability to bleach and dye, high-definition photos were taken with a mobile phone before and after the isolated white hair was soaked in the skin-growing oil preparation, and used for comparative analysis of the changes in white hair turning black.

[0058] The results are as follows Figure 5As shown, by comparing the left part of the figure (before immersion) with the right part of the figure (after immersion for 48 hours), there is no sign that the skin-regenerating oil preparation can turn the in vitro white hair black within 48 hours (according to the time of applying once a day and maintaining for 1 hour in Examples 3 and 4, the time of in vitro immersion for 48 hours is equivalent to the time of applying to the scalp and hair roots for 48 days). This shows that the skin-regenerating oil preparation that can turn white hair black in this application is not like the hair dye products sold and used on the market. It does not have the effect of dyeing white hair into black hair, indicating that it does not belong to hair dye (hair dyes on the market can usually achieve a rapid bleaching and dyeing effect within half an hour).

Claims

1. Use of a skin-regenerating oil preparation in the preparation of an external-use drug for preventing or treating non-bleached and dyed white hair to black hair, characterized in that: The skin-regenerating oil preparation is a skin-regenerating oil alcohol extract oil-in-water preparation; the content of the skin-regenerating oil alcohol extract in the skin-regenerating oil alcohol extract oil-in-water preparation is not less than 10 mg / ml and not more than 30 mg / ml; the preparation method of the skin-regenerating oil alcohol extract oil-in-water preparation is: The freeze-dried powder of the alcohol extract of raw skin oil and egg yolk lecithin are dissolved in edible vegetable oil to prepare an oil phase, and the oil phase is dropped into an aqueous phase prepared by dissolving Tween 60 and glycerol in ultrapure water at a volume ratio of 1:4, and then stirred and high-speed sheared and high-pressure homogenized to obtain; the obtained oil-in-water preparation of the alcohol extract of raw skin oil has uniform zeta potential and particle size distribution; The preparation method of the lyophilized powder of the alcohol extract of raw skin oil is as follows: taking dry powders of seven raw medicinal materials of raw skin oil and borneol according to a certain weight ratio and adding them into a container, then adding an ethanol solution with a volume fraction of 50%-90% into the container, soaking for 2-3 hours, flash extraction for 3-5 times, each time for 5 minutes, concentrating the filtrate, freeze-drying, and obtaining the lyophilized powder of the alcohol extract of raw skin oil; The seven raw materials of the skin-regenerating oil are astragalus, angelica, tangerine peel, scutellaria, coptis, phellodendron, and rhubarb; the weight proportions of each raw material and borneol are as follows: 5-30 weight parts of astragalus, 5-30 weight parts of angelica, 5-30 weight parts of tangerine peel, 5-30 weight parts of scutellaria, 5-30 weight parts of coptis, 5-30 weight parts of phellodendron, 5-30 weight parts of rhubarb, and 3-5 weight parts of borneol; The relationship between the total weight of the eight raw materials of the skin-generating oil and the amount of the ethanol solution with a volume fraction of 50%-90% is: 500g: (2.9-4.4) L; The gray hair is acquired gray hair.

2. The use according to claim 1, characterized in that: The preparation method of the oil-in-water preparation of the skin-regenerating oil alcohol extract comprises the following steps: 1) dissolving the freeze-dried powder of the alcohol extract of raw skin oil and egg yolk lecithin in sesame seed oil to prepare an oil phase; the final concentration of the egg yolk lecithin is 6% W / V; 2) dissolving Tween 60 and glycerol in ultrapure water to prepare an aqueous phase; the final concentration of Tween 60 is 1.2% V / V; the final concentration of glycerol is 2% V / V; 3) In a constant temperature water bath at 60° C., drop the oil phase prepared in step 1) into the water phase prepared in step 2), and stir at 1200 r / min for 15 min; the volume ratio of the oil phase to the water phase is 1:4; 4) High-speed shearing was performed at 10,000 rpm for 2 min, and then high-pressure homogenization was performed at 1,000 bar for 6 times to obtain an oil-in-water preparation of the alcohol extract of the raw skin oil.

3. The use according to any one of claims 1-2, characterized in that: The zeta potential and particle size distribution of the obtained oil-in-water preparation of the alcohol extract of raw skin oil are uniform. The particle size distribution is 141 ~ 825 nm, and there is no multiple scattering phenomenon. The average particle size is 290±10 nm; the average zeta potential is -50 mV~-40 mV.

4. The use according to claim 1, characterized in that: The dry powders of the seven Chinese medicinal materials, namely, astragalus, angelica, dried orange peel, scutellaria, coptis, phellodendron and rhubarb, are all sieved through a 200-mesh sieve.

5. The use according to claim 1, characterized in that: The edible vegetable oil is selected from at least one of sesame seed oil and olive oil.

6. The use according to claim 1, characterized in that: The content of the alcohol extract of raw skin oil in the oil-in-water preparation of alcohol extract of raw skin oil is 30 mg / ml.

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