A bactericidal composition and its use

Abstract

The present application belongs to the field of fungicide, discloses a kind of fungicide composition, and the active ingredient of the fungicide composition includes active ingredient A, active ingredient B and active ingredient C, the active ingredient A is ametoctradin, the active ingredient B is prothioconazole, and the active ingredient C is mancozeb.The present application is reasonable in component, has good fungicidal effect, and its activity and fungicidal effect are not simply superimposed each component activity, compared with the existing single preparation, in addition to having significant fungicidal effect, but also has significant synergistic effect, and good crop safety.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of fungicide, in particular to a fungicide composition and application thereof. BACKGROUND

[0002] Pydiflumetofen is a succinate dehydrogenase inhibitor fungicide developed by Syngenta Crop Protection AG. It is suitable for a variety of crops, especially the highest activity on leaf spot and powdery mildew, and is highly effective on diseases caused by pathogenic fungi such as grapevine, sclerotinia and claviceps, etc. Pydiflumetofen also breaks through the prevention and treatment of diseases caused by fusarium on cereals. Chemical name: 3-(difluoromethyl)-N-methyl-N-[1-methyl-2-(2,4,6-trichlorophenyl)ethyl]-1H-pyrazole-4-formamide, the chemical structure is as follows:

[0003]

[0004] Prothioconazole is a new triazole sulfone fungicide developed by Bayer in 2004. It inhibits the demethylation of lanosterol precursor and interferes with the biosynthesis of sterols, thereby playing a fungicidal role. Prothioconazole has good systemicity, unique mechanism of action, excellent protection, treatment and eradication activity, and wide fungicidal spectrum. It is mainly used in cereal crops such as wheat, barley, rice, peanut, rapeseed and legumes, etc. to prevent and treat various diseases. Chemical name: 2-[2-(1-chlorocyclopropyl)-3-(2-chlorophenyl)-2-hydroxypropyl]-1H-1,2,4-triazole-3-thione, the chemical structure is as follows:

[0005]

[0006] Mancozeb is a broad-spectrum and highly effective fungicide, which can prevent and treat various diseases of crops, vegetables and fruit trees such as cucumber, peanut, potato, rice, tomato, grape, pear, apple, corn, etc. It is one of the most widely used protective fungicides in agricultural production. Due to years of use, some diseases have developed resistance to mancozeb.

[0007] The applicant accidentally found that the combination of pydiflumetofen, prothioconazole and mancozeb with different mechanisms of action for preventing and treating plant diseases has good fungicidal effect, and the activity and fungicidal effect are not a simple addition of the activity of each component. Compared with existing single preparations, it has not only significant fungicidal effect, but also significant synergistic effect, and is safe to crops. SUMMARY

[0008] Based on the above, the present application aims to provide a fungicidal composition, the active ingredient of which comprises pyroquilon, prothioconazole and mancozeb. The components of the present application are reasonable, the fungicidal effect is good, and the activity and fungicidal effect are not a simple superposition of the activity of each component. Compared with the existing single preparation, in addition to having a significant fungicidal effect, it also has a significant synergistic effect, and is safe for crops.

[0009] Another object of the present application is to provide a fungicidal composition containing pyroquilon, prothioconazole and mancozeb.

[0010] Another object of the present application is to provide the use of a fungicidal composition containing pyroquilon, prothioconazole and mancozeb in the prevention and treatment of plant diseases.

[0011] In order to achieve the above-mentioned object, the following technical scheme is provided: a fungicidal composition, the active ingredient of which comprises active ingredient A, active ingredient B and active ingredient C, the active ingredient A is pyroquilon, the active ingredient B is prothioconazole, and the active ingredient C is mancozeb.

[0012] Further, the mass ratio of the active ingredient A, the active ingredient B and the active ingredient C is (1-15):(1-15):(10-100);

[0013] Further, the mass ratio of the active ingredient A, the active ingredient B and the active ingredient C is (1-10):(1-10):(20-60) or any value between the above-mentioned values;

[0014] Further, the mass ratio of the active ingredient A, the active ingredient B and the active ingredient C is:

[0015]

[0016]

[0017] Further, the mass ratio of the active ingredient A, the active ingredient B and the active ingredient C is:

[0018] mass ratio active ingredient A active ingredient B active ingredient C A:B:C 1 1 30 A:B:C 1 2 25 A:B:C 5 14 175 A:B:C 12 35 375 A:B:C 12 35 450 A:B:C 1 3 35 A:B:C 1 3 40 A:B:C 5 16 150 A:B:C 5 16 175 A:B:C 2 7 50 A:B:C 2 7 60

[0019] Further, the total content of the active ingredients in the fungicidal composition is 1-90wt% based on 100wt% of the total weight of the fungicidal composition;

[0020] Further, the total content of the active ingredients in the fungicidal composition is 5-80wt%;

[0021] Further, the content of the active ingredients in the fungicidal composition is 29wt%, 34wt%, 49.7wt%, 55wt%, 59wt%, 68wt%.

[0022] Further, the fungicidal composition comprises, in addition to the active ingredients, agriculturally acceptable auxiliary ingredients selected from one or more of wetting agents, dispersants, emulsifiers, thickening agents, disintegrants, antifreezing agents, antifoaming agents, solvents, preservatives, stabilizers, synergists or carriers.

[0023] The wetting agent is selected from one or more of alkyl benzene sulfonate, alkyl naphthalene sulfonate, lignin sulfonate, sodium dodecyl sulfate, dioctyl phthalate sulfonate sodium, alpha olefin sulfonate, alkyl phenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkyl phenol ethoxylate, fatty alcohol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm excrement, soap nut powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0024] The dispersant is selected from one or more of lignin sulfonate, alkyl naphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, styryl phenol ethoxylate phosphate, fatty alcohol ethoxylate, alkyl phenol polyoxyethylene ether, alkyl phenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylate, polyacrylate, phosphate, EO-PO block copolymer and EO-PO graft copolymer; and / or

[0025] The emulsifier is selected from one or more of calcium dodecyl benzene sulfonate, alkyl phenol formaldehyde resin polyoxyethylene ether, phenylethyl phenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styryl phenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkyl phenol ether phosphate; and / or

[0026] The thickening agent is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and / or

[0027] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0028] The antifreezing agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0029] The antifoaming agent is selected from one or more of C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10One or more of the fatty alcohols; and / or

[0030] The solvent is selected from one or more of benzene, toluene, xylene, mesitylene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, hydrocarbon carbonates, diesel oil, solvent oil, vegetable oil, vegetable oil derivatives, and water; and / or

[0031] The preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, Kathon, and 1,2-benzisothiazolin-3-one; and / or

[0032] The stabilizer is selected from one or more of the following: disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, silica, talc, montmorillonite, and starch; and / or

[0033] Synergists are selected from synergistic phosphorus, synergistic ether; and / or

[0034] The carrier is selected from one or more of the following: ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils, and vegetable oil derivatives.

[0035] Furthermore, the bactericidal composition can be prepared into agriculturally permissible formulations, wherein the formulations are selected from solid and / or liquid formulations;

[0036] The solid dosage forms include powders, granules, balls, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets, or soluble granules.

[0037] The liquid formulations include soluble agents, colloids, oils, spreading oils, emulsifiable concentrates, latexes, dispersible liquids, ointments, water emulsions, oil emulsions, microemulsions, lipids, suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspensions, microcapsule suspension-suspensions, microcapsule suspension-water emulsions, or microcapsule suspension-suspension emulsions.

[0038] Furthermore, the solid formulation is selected from wettable powders and water-dispersible granules; the liquid formulation is selected from suspension concentrates and dispersible oil suspensions.

[0039] The present invention also discloses the application of the above-described bactericidal composition for the prevention and control of plant diseases;

[0040] Furthermore, the plants mentioned are crops, vegetables, and fruit trees.

[0041] Further, the plant disease is caused by Basidiomycetes or Mitosporic fungi;

[0042] Further, the Basidiomycetes is Puccinia or Phakopsora, and the Mitosporic fungi is Pyricularia, Drechslera, Exserohilum, Cercospora, Rhizoctonia or Corynespora;

[0043] Further, the plant disease is wheat leaf rust (Puccinia recondita f.sp.tritici), wheat stripe rust (Puccinia striiformis f.sp.tritici), soybean rust (Phakopsora pachyrhizi), rice blast (Pyricularia oryzae), corn southern blight (Exserohilum turcicum), soybean brown spot (Cercospora sojina), rice sheath blight (Rhizoctonia solani), cucumber target spot (Corynespora cassiicola), peanut black spot (Cercospora personata) or peanut brown spot (Corynespora arachidicola).

[0044] Further, the fungicidal composition or the preparation thereof is applied on the disease to be controlled or the growth medium thereof in an effective dose.

[0045] The present application has good control effect on the plant disease caused by Basidiomycetes or Mitosporic fungi, low cost, and the activity and fungicidal effect are not the simple superposition of the activity of each component. Compared with the existing single preparation, the present application has significant synergistic effect, is safe to crops, and meets the safety requirements of pesticide preparation. DETAILED DESCRIPTION

[0046] In order to make the technical solutions, purposes and advantages of the present application clearer and more apparent, the present application is described in the following specific embodiments, but the present application can be implemented in various forms and should not be limited by the embodiments described herein.

[0047] Specific preparation examples:

[0048] Preparation Example 1:

[0049] 34% fluoxastrobin+propiconazole+mancopper suspension concentrate (3+3+28)

[0050] Formula composition: fluazinam 3%, prothioconazole 3%, mancozeb 28%, isomeric tridecanol polyoxyethylene ether 1.2%, naphthalene sulfonate formaldehyde condensate 0.5%, styrene phenol polyoxyethylene ether phosphate ester salt 3%, EO / PO block polyether 2%, xanthan gum 0.2%, magnesium aluminum silicate 0.5%, glycerol 5%, benzoisothiazolinone 0.01%, silicone oil 0.5%, deionized water to make up the balance;

[0051] Preparation method: mix water, dispersant and wetting agent uniformly, add defoaming agent and antifreeze agent in turn under stirring, mix uniformly by high-speed shearing, then add the original drug, continue to shear and mix uniformly, then enter the horizontal sand mill for grinding, control the particle size of the material to be below 5μm, finally add thickening agent, shear uniformly to obtain the suspension agent.

[0052] Preparation example 2:

[0053] 49.7% fluazinam·prothioconazole·mancozeb dispersible oil suspension (1.2+3.5+45)

[0054] Formula composition: fluazinam 1.2%, prothioconazole 3.5%, mancozeb 45%, triphenyl ethenyl phenol polyoxyethylene ether 7.5%, alkyl phenol polyoxyethylene ether 7%, calcium dodecylbenzenesulfonate 2%, solvent oil 12%, fumed white carbon black 0.5%, organic bentonite 0.2%, methyl oleate to make up 100%;

[0055] Preparation method: mix methyl oleate and wetting dispersant uniformly, then add the original drug, continue to shear and mix uniformly, then enter the horizontal sand mill for grinding, control the particle size of the material to be below 5μm, finally mix uniformly to obtain the dispersible oil suspension.

[0056] Preparation example 3:

[0057] 29% fluazinam·prothioconazole·mancozeb dispersible oil suspension (1.5+3.5+25)

[0058] Formula composition: fluazinam 1.5%, prothioconazole 3.5%, mancozeb 25%, triphenyl ethenyl phenol polyoxyethylene ether 8%, alkyl phenol polyoxyethylene ether 6%, calcium dodecylbenzenesulfonate 2%, solvent oil 15%, fumed white carbon black 0.5%, organic bentonite 0.2%, methyl oleate to make up 100%;

[0059] Preparation method: same as preparation example 2.

[0060] Preparation example 4:

[0061] 68% of myclobutanil · prothioconazole · mancozeb wettable powder (3+9+56)

[0062] Formulation composition: myclobutanil 3%, prothioconazole 9%, mancozeb 56%, tea saponin 10%, naphthalene sulfonate formaldehyde condensate 3%, polycarboxylic acid sodium salt 2%, sodium dodecyl sulfate 2%, kaolin to make up the balance.

[0063] Preparation method: the active ingredients myclobutanil and pyraclostrobin are mixed with dispersants, wetting agents and fillers, uniformly stirred in a stirred tank, and uniformly mixed by airflow crusher for multiple times. The wettable powder of the present application can be prepared.

[0064] Preparation Example 5:

[0065] 59% of myclobutanil · prothioconazole · mancozeb water dispersible granules (2+7+50)

[0066] Formulation composition: myclobutanil 2%, prothioconazole 7%, mancozeb 50%, naphthalene sulfonate formaldehyde condensate 10%, sodium lignosulfonate 5%, polycarboxylic acid sodium salt 2%, sodium dodecyl sulfate 2.5%, kaolin to make up the balance.

[0067] Preparation method: the active ingredients and dispersants, wetting agents and fillers are mixed, uniformly stirred in a stirred tank, and then 10-25% water is added after airflow crushing. The water dispersible granule product is prepared by kneading, granulating, drying and screening; or the crushed powder is sprayed with water in a fluidized bed granulator, granulated, dried, and then screened to prepare the product.

[0068] Preparation Example 6:

[0069] 55% of myclobutanil · prothioconazole · mancozeb water dispersible granules (1.5+3.5+50)

[0070] Formulation composition: myclobutanil 1.5%, prothioconazole 3.5%, mancozeb 50%, naphthalene sulfonate formaldehyde condensate 10%, sodium lignosulfonate 3.5%, polycarboxylic acid sodium salt 2%, sodium dodecyl sulfate 2.5%, kaolin to make up the balance.

[0071] Preparation method: same as Preparation Example 5.

[0072] Laboratory toxicity determination

[0073] Laboratory Example 1

[0074] Phakopsora pachyrhizi - pot method

[0075] Mixing test reference: NY / T 1156.6-2006 "Guidelines of Pesticide Laboratory Bioassay Test Part 6: Joint Action of Fungicides in Mixtures".

[0076] Test agents: 98% myclobutanil, 95% prothioconazole, 88% mancozeb, provided by the Group R&D Center

[0077] Material preparation: Select soybean seedlings of uniform growth (2 true leaves) and susceptible variety for standby.

[0078] Agent preparation: Dissolve the test agent in acetone first, then dilute with 0.05% Tween 80 aqueous solution to prepare single agent stock solution, and set 5 series of mass concentrations according to the purpose of mixing and the activity of the agent.

[0079] Application method: Spray treatment, inoculate spore suspension 24h after agent treatment, inoculation concentration is 5×10 5 mL,

[0080] After inoculation, place in an artificial climate incubator, humidity 100% in the first 24h, dark culture, 24h later, temperature control at 25±1℃, relative humidity 80%-90%, light / dark ratio 12h / 12h, culture for 12-15d.

[0081] Disease classification refers to GB / T 17980.89-2004 "Guidelines for Field Efficacy Trials (II) Fungicides Part 89: Fungicides for Soybean Rust Control", calculate control effect by disease index.

[0082] Specifically: Classification standard

[0083] 0: No disease;

[0084] 1: Disease area accounts for less than 5% of the entire leaf area;

[0085] 3: Disease area accounts for 6%-25% of the entire leaf area;

[0086] 5: Disease area accounts for 26%-50% of the entire leaf area;

[0087] 7: Disease area accounts for 51%-75% of the entire leaf area;

[0088] 9: Disease area accounts for more than 76% of the entire leaf area.

[0089] Pharmacodynamic calculation method:

[0090]

[0091]

[0092] Test statistics: The data were processed by the method of probability value analysis. DPS statistical analysis system was used to analyze the data, and the toxicity regression line, R, EC 50 values were calculated to evaluate the activity of the test agent on the biological test material.

[0093] Wadley method: The synergistic effect of the mixed agent was evaluated according to the synergistic coefficient (SR).

[0094] The synergistic coefficient (SR) was calculated according to the following formula:

[0095]

[0096] In the formula:

[0097] X1 - EC 50 theoretical value of the mixed agent, unit: milligrams per liter (mg / L);

[0098] P A - the percentage content of A in the mixed agent, unit: percentage (%);

[0099] P B - the percentage content of B in the mixed agent, unit: percentage (%);

[0100] P C - the percentage content of C in the mixed agent, unit: percentage (%);

[0101] A - EC 50 value of A in the mixed agent, unit: milligrams per liter (mg / L);

[0102] B - EC 50 value of B in the mixed agent, unit: milligrams per liter (mg / L);

[0103] C - EC 50 value of C in the mixed agent, unit: milligrams per liter (mg / L);

[0104]

[0105] In the formula:

[0106] SR - synergistic coefficient of the mixed agent;

[0107] X1 - EC 50 theoretical value of the mixed agent, unit: milligrams per liter (mg / L);

[0108] X2 - EC 50 measured value of the mixed agent, unit: milligrams per liter (mg / L).

[0109] The synergistic coefficient (SR) of the mixed agent was calculated according to the Wadley method:

[0110] SR≥1.5 indicates a synergistic effect; SR≤0.5 indicates an antagonistic effect; and 0.5

[0111] Test results:

[0112] From the indoor activity test (see Table 1), the synergistic coefficient SR of the mixture of amisolan, prothioconazole and mancozeb against soybean rust EC 50 0.084 mg / L, 0.159 mg / L and 3.840 mg / L, respectively. The synergistic coefficient SR of the mixture of amisolan, prothioconazole and mancozeb (1:2:25, 5:14:175, 12:35:375, 12:35:450, 1:3:35, 1:3:40), 5:16:150, 5:16:175) against soybean rust was greater than 1.5, showing a synergistic effect.

[0113] Table 1 Indoor synergistic effect test results of the mixture of amisolan, prothioconazole and mancozeb against soybean rust

[0114]

[0115] Indoor Example 2

[0116] Corynespora arachidicola - plate method

[0117] Test basis: The test refers to NY / T 1156.2-2006 "Pesticide Indoor Biological Test Guidelines Fungicides Part 2: Inhibition of Mycelial Growth Test Plate Method" and NY / T 1156.6-2006 "Pesticide Indoor Biological Test Guidelines Fungicides Part 6: Synergistic Effect Test of Mixtures".

[0118] Test agents: 98% amisolan technical, 95% prothioconazole technical and 88% mancozeb technical, provided by the Group R&D Center.

[0119] Agent preparation: The test technical was first dissolved in acetone, and then diluted with 0.1% Tween 80 aqueous solution. Single-agent stock solutions were prepared, and five series of mass concentrations were set according to the purpose of mixing and the activity of the agent, with the final content of organic solvent not exceeding 2%.

[0120] After melting the PDA medium in a microwave oven, it was cooled to about 50°C. According to the principle of low concentration to high concentration, 1 mL of the prepared test solution and 9 mL of PDA medium were added to a culture dish with a diameter of 9 cm, mixed well, and the corresponding concentration of the drug-containing plate was prepared.

[0121] Inoculation: The pathogenic fungi were inoculated on the medium with a 6mm puncher under sterile conditions. After the medium solidified, the fungus cake was placed in the center of the medium. The Petri dish was sealed with sealing film and incubated at 27℃. The blank solution without the fungicide was used as a control. Each treatment was repeated 4 times.

[0122] Investigation: The colony diameter was measured with a caliper after 12 days of culture. The diameter was measured vertically with a cross method and the average value was obtained.

[0123] Data statistics and analysis: The mycelial growth of the pathogenic fungi was investigated according to the growth of the fungi in the blank control Petri dish. The colony diameter was measured with a cross method.

[0124] According to the investigation results, the mycelial growth inhibition rate of the test target fungi was calculated for each treatment concentration, which was expressed as a percentage (%). The calculation results were rounded to two decimal places.

[0125] D=D1-D2

[0126] In the formula:

[0127] D — colony growth diameter;

[0128] D1 — colony diameter;

[0129] D2 — fungus cake diameter.

[0130]

[0131] I — mycelial growth inhibition rate;

[0132] D0 — blank control colony growth diameter;

[0133] D t — fungicide-treated colony growth diameter.

[0134] Test statistics: The data was processed using the probability value analysis method. The DPS statistical analysis system was used for analysis to obtain the virulence regression line, R, EC 50 value, and evaluate the activity of the test fungicide on the biological test material.

[0135] Wadley method: The synergistic effect of fungicide mixing was evaluated according to the synergistic coefficient (SR).

[0136] The synergistic coefficient (SR) was calculated according to the following formula:

[0137]

[0138] In the formula:

[0139] X1 - EC of the mixture 50 Theoretical value in milligrams per liter (mg / L);

[0140] P A Percentage content of A in the mixture, in percent (%);

[0141] P B Percentage content of B in the mixture, in percent (%);

[0142] P C Percentage content of C in the mixture, in percent (%);

[0143] A - EC of A in the mixture 50 Value in milligrams per liter (mg / L);

[0144] B - EC of B in the mixture 50 Value in milligrams per liter (mg / L);

[0145] C - EC of C in the mixture 50 Value in milligrams per liter (mg / L);

[0146]

[0147] In the formula:

[0148] SR - Synergistic coefficient of the mixture;

[0149] X1 - EC of the mixture 50 Theoretical value in milligrams per liter (mg / L);

[0150] X2 - EC of the mixture 50 Measured value in milligrams per liter (mg / L).

[0151] The synergistic coefficient (SR) of the mixture is calculated according to the Wadley method. SR > 1.5 indicates synergistic effect; SR < 0.5 indicates antagonism; 0.5 < SR < 1.5 indicates additive effect.

[0152] Test results:

[0153] From the indoor activity test (see Table 2), it can be seen that fluoxastrobin, prothioconazole and mancozeb have synergistic effect on peanut brown spot disease EC 500.072 mg / L, 0.577 mg / L, 63.268 mg / L. The synergistic coefficients SR of pydiflumetofen, prothioconazole and mancozeb mixed at 1:2:25, 5:14:175, 12:35:375, 12:35:450, 1:3:35, 1:3:40, 5:16:150, 5:16:175 against peanut brown spot were all greater than 1.5, showing synergistic effect.

[0154] Table 2 In vitro combined action test results of pydiflumetofen, prothioconazole and mancozeb against peanut brown spot

[0155]

[0156]

[0157] Field efficacy test

[0158] Field example 1

[0159] Field efficacy test of pydiflumetofen, prothioconazole and mancozeb against peanut leaf spot

[0160] Test basis: Refer to GB / T 17980.89-2004 "Guidelines for Field Efficacy Test (II) Fungicides Part 85: Fungicides for Controlling Peanut Leaf Spot".

[0161] Test crop: peanut (Lu Hua No. 8)

[0162] Test site: Jining Jinxiang, Shandong, the previous crop of the test site was peanut, winter fallow, and the field soil was light sandy soil with an organic matter content of 1.8 g / kg.

[0163] Test site conditions: Sowing in mid-April 2021.

[0164] Test design: The test plots were arranged in random blocks, and the plot area was 25 m 2 , repeated 4 times per plot.

[0165] Application time: Uniformly spray the plants with Gongnong 16 type knapsack sprayer, and the application liquid amount is 45 kg / acre. The test was carried out on June 30, 2021 for the first time (flowering and needle stage), and then once every 7 days, a total of two times, and the control area was sprayed with water.

[0166] Test treatment and dosage:

[0167] Table 3 Test pesticides and dosages

[0168]

[0169]

[0170] Test investigation: Investigate the disease base before application, and investigate the disease condition, diseased leaf number and plant overall growth after 7 days of each application. Randomly sample 5 points per plot before the first application, investigate 3 plants per point, and randomly investigate the upper, middle and lower 3 compound leaves per plant to investigate the occurrence of peanut leaf spot in each treatment. Record the total leaf number and the number of diseased leaves at each level 7 days after the first application, 10 days after the first application and 10 days after the second application, and calculate the disease index and control effect.

[0171] Specifically:

[0172] Classification standard

[0173] 0 level: no disease;

[0174] 1 level: the area of disease spot is less than 5% of the entire leaf area;

[0175] 3 level: the area of disease spot is 6% to 25% of the entire leaf area;

[0176] 5 level: the area of disease spot is 26% to 50% of the entire leaf area;

[0177] 7 level: the area of disease spot is 51% to 75% of the entire leaf area;

[0178] 9 level: the area of disease spot is more than 76% of the entire leaf area;

[0179] Method for calculating efficacy:

[0180]

[0181]

[0182] Safety investigation: During the test, the peanut plants in each treatment plot grew well, and the test agents at the test concentration did not cause phytotoxicity to the peanut plants and other non-target organisms.

[0183] Yield measurement: Field yield measurement was performed during the peanut harvesting period, and sample points were randomly selected for each treatment plot. Five samples were taken per sample point, and the sample area was 1m 2 . Double rows of plants were taken, the number of peanut plants per mu was calculated, the peanut plants in the sample points were pulled out, all the pods were removed, and the weight was measured. The yield per mu was converted.

[0184] The results of the field efficacy test are shown in the following table:

[0185] The field efficacy showed (see Table 4): Under the same environmental conditions, the plant height of different treatments for controlling peanut leaf spot was 10.28-11.97 cm 7 days after the first application, and there were differences between the treatments.

[0186] Table 4 Field efficacy test results of peanut leaf spot

[0187]

[0188] The field efficacy shows (see Table 5): the overall control effect of each treatment in the field is 61.75%~89.55% at 10 days after the first application. Among them, 49.7% pydiflumetofen + prothioconazole + mancozeb dispersible oil suspension (1.2+3.5+45), 55% pydiflumetofen + prothioconazole + mancozeb water dispersible granules (1.5+3.5+50) two ternary mixture treatments show good control effect, the control effect is 89.06%, 89.55% respectively, and are better than the conventional pesticides 25% pyraclostrobin suspension and the control pesticides (25% pydiflumetofen + prothioconazole (10+15) dispersible oil suspension, 20% pydiflumetofen + mancozeb wettable powder (10+10), 44% mancozeb + prothioconazole suspension, 200g / L pydiflumetofen suspension, 480g / L prothioconazole suspension, 80% mancozeb wettable powder).

[0189] Table 5 The field efficacy of each treatment of test pesticide compound on peanut leaf spot at 10 days after the first application

[0190]

[0191]

[0192] Note: lowercase letters represent 5% level of significant difference, and uppercase letters represent 1% level of significant difference.

[0193] The field efficacy shows (see Table 6): the overall control effect of each treatment in the field is increased, and the control effect is 78.29%~94.36% at 10 days after the second application. 49.7% pydiflumetofen + prothioconazole + mancozeb dispersible oil suspension (1.2+3.5+45), 55% pydiflumetofen + prothioconazole + mancozeb water dispersible granules (1.5+3.5+50) show good persistence, and the control effect is 94.36%, 93.95% respectively.

[0194] Table 6 The field efficacy of each treatment of test pesticide compound on peanut leaf spot at 10 days after the second application

[0195]

[0196]

[0197] Note: lowercase letters represent 5% level of significant difference, and uppercase letters represent 1% level of significant difference.

[0198] The field efficacy shows (see Table 7) that in terms of yield, 49.7% lufenuron·prothioconazole·manganese ethylene bisdithiocarbamate dispersible oil suspension (1.2+3.5+45), 55% lufenuron·prothioconazole·manganese ethylene bisdithiocarbamate water dispersible granules (1.5+3.5+50) can increase the yield by more than 30% compared with the blank control.

[0199] Table 7 Yield-increasing effect of the pesticide on the control of peanut leaf spot

[0200]

[0201] Through indoor toxicity determination and field tests, the pesticide composition prepared by compounding lufenuron, prothioconazole and manganese ethylene bisdithiocarbamate according to the present application shows good control effect on peanut leaf spot.

[0202] The prepared pesticide composition or its preparation has significant control effect, is superior to single agent in delaying the generation of drug resistance and prolonging the effective period. In addition, no drug damage to crops is found in the test, which shows that in the case of synergistic effect of the prepared pesticide composition or its preparation, the production cost and use cost can be reduced, and the crops are safe.

[0203] Although the present application has been described in detail by general description and specific embodiments above, some modifications or improvements can be made on the basis of the present application, which is obvious to those skilled in the art, and therefore, these modifications or improvements made on the basis of not deviating from the spirit of the present application all belong to the scope of protection required by the present application.

Claims

1. A fungicidal composition, characterized by, The active ingredients of the fungicidal composition consist of active ingredient A, active ingredient B and active ingredient C, the active ingredient A is pydiflumetofen, the active ingredient B is prothioconazole, and the active ingredient C is mancozeb, and the mass ratio of the active ingredient A, the active ingredient B and the active ingredient C is 1:2:25, 5:14:175, 12:35:375, 12:35:450, 1:3:35, 1:3:40, 5:16:150, 5:16:

175.

2. The bactericidal composition according to claim 1, characterized by The total content of the active ingredients in the fungicidal composition is 1-90wt% based on the total weight of the fungicidal composition being 100wt%.

3. The germicidal composition according to claim 2, characterized in that, The total content of the active ingredients in the fungicidal composition is 5-80wt%.

4. The germicidal composition according to claim 1, wherein The fungicidal composition further comprises one or more of the following agriculturally acceptable auxiliary ingredients in addition to the active ingredients: wetting agents, dispersants, emulsifiers, thickening agents, disintegrating agents, antifreezing agents, antifoaming agents, solvents, preservatives, stabilizers, synergists or carriers.

5. The germicidal composition according to claim 1, wherein The fungicidal composition is prepared into an agriculturally acceptable formulation form, which is selected from solid formulations and / or liquid formulations; the solid formulation is selected from wettable powder and water dispersible granule; the liquid formulation is selected from suspension concentrate and dispersible oil suspension.

6. Use of a fungicidal composition according to any one of claims 1 to 5 for the control of plant diseases, characterized in that, The plant diseases are soybean rust and peanut brown spot.

7. Use according to claim 6, characterized in that, The fungicidal composition is applied to the diseases to be controlled or the growth medium thereof in an effective amount.

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