A traditional Chinese medicine composition for resisting chicken coccidiosis and a preparation method thereof

Abstract

This invention relates to the field of veterinary medicine technology and provides a traditional Chinese medicine composition for treating coccidiosis in chickens, along with its preparation method. The composition is prepared from a combination of raw materials including *Gnaphalium affine*, *Hedyotis diffusa*, *Dendrobium nobile*, *Hemiberlesia argyi*, and *Thalictrum sylvestris*. This pure traditional Chinese medicine preparation exhibits no drug resistance, no drug residues, no toxic side effects, and no palatability issues. It also possesses advantages such as significant efficacy, rational formulation, simple preparation, low cost, and environmental friendliness. The traditional Chinese medicine composition of this invention has the effects of stopping diarrhea and killing parasites, clearing heat and drying dampness, and tonifying the middle and replenishing qi. It has a significant effect against coccidiosis in chickens, effectively preventing abnormal feces, intestinal bleeding, and intestinal ulcers caused by coccidiosis, and rapidly restoring the production performance of chicken flocks, thus improving breeding profits.

Description

Technical Field

[0001] This invention belongs to the field of veterinary drug technology, specifically relating to a traditional Chinese medicine composition for treating coccidiosis in chickens and its preparation method. Background Technology

[0002] As is well known, coccidiosis in chickens is a common and highly damaging parasitic disease, posing a serious threat to poultry farming. It is caused by one or more species of coccidia in the genus *Eimeria* that parasitize the intestinal epithelial cells of chickens. *Eimeria tenella* is the most damaging and pathogenic species, with a morbidity rate as high as 50%-70%. The mortality rate of infected chickens is close to 20-40%, and in severe cases, it can exceed 60%. It can cause chicken mortality, slow growth, decreased egg production, reduced feed utilization, and increased feeding costs.

[0003] Coccidiosis in chickens is prevalent, especially in free-range or floor-raised chicken farms, and tends to recur. In housed chicken farms, the disease can occur year-round. Chicks aged 15-70 days are most susceptible, with outbreaks typically occurring in chickens aged 3-6 weeks, and it is more prevalent in warm, humid seasons. Infected chickens are the primary source of infection. Their feces spread through the chicken coop, cages, feed, feeding equipment, and via rodents and flies. Symptoms include loss of appetite, huddling together, closed eyes and retracted neck, bloody stools, and death. After infection, the coccidia primarily parasitize the small intestine and cecum. The cecum becomes highly swollen, with numerous small, hard, white nodules on the vermiformes, sometimes showing purulent necrotic foci. The small intestine is filled with gas and mucus, and the intestinal lumen is filled with blood clots and sloughed tissue fragments. Subsequently, a red or red-white intestinal core forms; later, it sloughs off from the mucosa, the cecum atrophies and shortens, and the intestinal wall thickens.

[0004] Based on the pathogenic conditions, symptoms, and necropsy findings of chickens, many scholars in Traditional Chinese Veterinary Medicine agree that chicken coccidiosis is caused by damp-heat and external parasitic pathogens, resulting in damp-heat dysentery and parasitic infestation. In the Eight Principles of Differentiation, it is further differentiated as a condition that progresses from excess to deficiency. Chicken coccidiosis often occurs in hot and humid climates. Not only does the parasitic pathogen damage yin and deplete blood and fluids, but the internal damp-heat also weakens the spleen yang. Therefore, in the early stages, affected chickens exhibit reduced appetite, lethargy, loose stools with blood, and thirst. Later stages show poor blood circulation, pale combs, emaciation, and ultimately death. Treatment focuses on stopping diarrhea, killing parasites, clearing heat and drying dampness, and tonifying the middle jiao and replenishing qi.

[0005] Coccidiosis in chickens causes significant economic losses to the poultry industry. Currently, clinical treatment still primarily relies on chemical anticoccidial drugs, especially as feed additives. However, long-term use of chemical drugs not only leads to drug resistance in chicken coccidia but also exhibits strong hepatotoxicity and nephrotoxicity when used in chicks, severely harming animal growth. Furthermore, the residue of chemical drugs in meat or eggs ends up on the dinner table, seriously impacting human health. Traditional Chinese medicine possesses unique therapeutic advantages. Utilizing the non-toxic side effects, no drug restriction period, no residue, and environmental friendliness of herbal preparations, replacing chemical drugs in the poultry industry is an inevitable trend. This study aims to provide a traditional Chinese medicine preparation for treating chicken coccidiosis and to offer a reference for using traditional Chinese medicine as an alternative to antibiotics and for treating chicken coccidiosis with traditional Chinese medicine. Summary of the Invention

[0006] To address the shortcomings of existing technologies and meet clinical needs, this invention provides a traditional Chinese medicine composition for treating coccidiosis in chickens that is highly effective, rationally formulated, simple to prepare, and low in cost, as well as its preparation method.

[0007] To achieve the above objectives, the technical solution proposed by this invention is as follows:

[0008] A traditional Chinese medicine composition for treating coccidiosis in chickens and its preparation method thereof, wherein the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10-20 parts of *Gynostemma pentaphyllum*, 5-15 parts of germinated barley, 20-35 parts of *Toona sinensis* leaves, 5-15 parts of lotus seeds, 10-20 parts of *Hedyotis diffusa*, 5-15 parts of *Dendrobium nobile*, 20-30 parts of *Polygonum hydropiper*, 10-20 parts of *Thalictrum cylindrica*, and 3-10 parts of licorice.

[0009] Preferably, the traditional Chinese medicine composition is made from the following raw materials in parts by weight: 12-18 parts of *Gynostemma pentaphyllum*, 6-13 parts of germinated barley, 25-32 parts of *Toona sinensis* leaves, 8-13 parts of lotus seeds, 12-18 parts of *Hedyotis diffusa*, 8-13 parts of *Dendrobium nobile*, 22-28 parts of *Polygonum hydropiper*, 12-18 parts of *Thalictrum cylindrica*, and 5-8 parts of licorice.

[0010] More preferably, the traditional Chinese medicine composition is made from the following raw materials in parts by weight: 15 parts of Venus simonii, 10 parts of germinated barley, 30 parts of Toona sinensis leaves, 10 parts of lotus seeds, 15 parts of Water lily, 10 parts of Dendrobium, 25 parts of Firethorn, 15 parts of Thalictrum lucidum, and 6 parts of licorice.

[0011] The traditional Chinese medicine composition is made from the following raw materials in parts by weight: 15 parts of Venus simonii, 10 parts of germinated barley, 25 parts of Toona sinensis leaves, 10 parts of lotus seeds, 15 parts of Water lily, 10 parts of Dendrobium, 23 parts of Fire vine, 15 parts of Thalictrum lucidum, and 6 parts of licorice.

[0012] More preferably, the traditional Chinese medicine composition is characterized in that: the traditional Chinese medicine composition is a powder, oral liquid, granule, pill or tablet.

[0013] More preferably, the dosage form of the traditional Chinese medicine composition is a powder.

[0014] The preparation method for the formulation used to prepare the above-mentioned traditional Chinese medicine composition is as follows:

[0015] The first step is to weigh each raw material according to the stated weight proportions; wash, dry, cut into sections, crush and pass through a No. 2 sieve, mix evenly, put into an extraction tank, add an appropriate amount of water to submerge the medicinal materials by 2-5 cm, and soak for 1 hour;

[0016] The second step involves heating the Chinese medicinal materials from the first step to 40-60℃, adding 0.2-0.5% cellulase, adjusting the pH to 4.5-6.0, and hydrolyzing for 3-5 hours while stirring continuously.

[0017] The third step is to add water at 10-12 times the total weight of all the herbs after enzymatic hydrolysis, bring to a boil, and then simmer for 1.5 hours.

[0018] The fourth step is to filter the Chinese medicine from the third step, and then add 8-10 times the total weight of the medicinal materials to the filter residue and decoct for 1 hour.

[0019] Fifth step: Filter the Chinese medicine from the fourth step, then add 7-9 times the total weight of the medicinal materials and decoct for 1 hour;

[0020] Step 6: Combine the decoctions from the three decoctions, concentrate them under reduced pressure to a relative density of 1.07-1.12, spray dry to obtain material 1; then freeze dry the filter residue after enzymatic hydrolysis under vacuum, pulverize it through an 80-100 mesh sieve to obtain material 2; mix material 1 and material 2 evenly, sterilize, and package to obtain the traditional Chinese medicine compound preparation of the present invention.

[0021] The present invention also provides the application of the aforementioned traditional Chinese medicine composition in the treatment of coccidiosis in chickens.

[0022] The medicinal properties and effects of each medicinal material in the herbal composition of this invention are as follows:

[0023] Venus Grass; Properties: Bitter; Cold. Channels Entered: Heart, Large Intestine, Small Intestine, Spleen. Functions and Indications: Clears heat, cools blood, detoxifies. Treats carbuncles, boils, scrofula, malignant sores, conjunctivitis, gonorrhea, and intestinal bleeding.

[0024] Germinated rice; Properties: neutral, sweet. Channels entered: stomach, spleen. Functions and effects: promotes digestion, harmonizes the stomach, strengthens the spleen and improves appetite. Used for indigestion, abdominal distension, halitosis, spleen and stomach weakness, and poor appetite. Fried germinated rice is more effective in promoting digestion and is used for poor appetite. Roasted germinated rice is good at resolving stagnation and is used for indigestion.

[0025] Toona sinensis leaves; Properties: neutral, bitter. Channels entered: Heart, Spleen, Large Intestine. Functions and effects: anti-inflammatory, detoxifying, and insecticidal. Treats enteritis, dysentery, boils, carbuncles, lacquer dermatitis, scabies, and alopecia areata.

[0026] Lotus seeds; Properties: neutral, sweet, astringent. Channels entered: heart, spleen, kidney. Functions: tonifies the spleen and stops diarrhea, benefits the kidneys and astringes essence, nourishes the heart and calms the mind. Used for chronic diarrhea due to spleen deficiency, seminal emission, leukorrhea, palpitations, and insomnia.

[0027] Water lily; Properties: neutral, bitter. Channels entered: spleen, liver, large intestine. Functions and effects: clears heat and removes dampness; disperses blood stasis and relieves pain; stops bleeding and promotes wound healing. Mainly used for dysentery; enteritis; edema; carbuncles and boils; eczema; non-healing ulcers; and traumatic bleeding.

[0028] Dendrobium; Properties: Slightly cold, sweet. Channels entered: Spleen, Stomach, Kidney. Functions and effects: Strengthens the spleen and stomach, nourishes yin and clears heat. It has certain effects on symptoms such as spleen deficiency and stomach heat. It can promote food digestion, help the spleen and stomach function, and is also beneficial for replenishing qi and nourishing blood.

[0029] Firefly; Properties: Cold, bitter. Channels: Heart, Large Intestine. Functions: Reduces swelling, promotes bowel movement, kills parasites. Treats abdominal distension, acute gastroenteritis, boils, and scabies.

[0030] Thalictrum lucidum; Properties: Cold, bitter. Channels entered: Heart, Large Intestine, Liver. Functions and effects: Clears heat and drains fire; dries dampness and detoxifies. Mainly used for feverish irritability; damp-heat diarrhea; lung-heat cough; red and swollen eyes; carbuncles and boils.

[0031] Licorice root; neutral in nature, sweet in taste. Meridians it enters: Heart, Stomach, Spleen, and Lung. Functions and effects: Tonifies the spleen and replenishes qi, relieves cough and eliminates phlegm, alleviates spasms and pain, and harmonizes the properties of other herbs.

[0032] The beneficial effects of this invention are as follows: In this formula, *Toona sinensis* leaves and *Polygonum hydropiper* are the principal herbs, working together to kill parasites and stop diarrhea. *Gynostemma pentaphyllum*, *Hylocereus undatus*, and *Thalictrum sylvestris* work together to clear heat and dry dampness, detoxify and reduce swelling, purge fire and stop diarrhea, enhancing the efficacy of the principal herbs; these are the assistant herbs in this formula. Germinated barley, lotus seeds, and *Dendrobium nobile* can tonify the spleen and stop diarrhea, promote digestion and harmonize the middle jiao, and replenish qi and vital energy; these are the adjuvant herbs in this formula. Licorice root tonifies the spleen and replenishes qi, moistens the lungs, relieves spasms and pain, moderates the properties of the herbs, and harmonizes the effects of all the herbs; this is the guiding herb. This invention is based on the principles of syndrome differentiation and treatment in traditional Chinese veterinary medicine, rationally combining herbs to fully utilize their effects. The combined use of these herbs synergistically enhances the overall efficacy, possessing the effects of stopping diarrhea and killing parasites, clearing heat and drying dampness, and tonifying the middle jiao and replenishing qi. It has a significant effect on the treatment of coccidiosis in chickens. Attached Figure Description

[0033] Figures A1-A6 show the bloody stools of chickens in the control group after modeling in Experiment 1 of this invention;

[0034] Figures B1-B6 show the cecal lesions of chickens in the infected control group after modeling in Experiment 1 of this invention.

[0035] Figures C1-C2 show the clinical symptoms of test group 2 before the experiment in experiment 2 of this invention;

[0036] Figures D1-D2 show the results after the experiment in test group 2 of experiment 2 of the present invention;

[0037] Figures E1-E2 show the clinical symptoms of test group 1 before the test in test 3 of this invention;

[0038] Figures F1-F2 show the results of the experiment in test group 1 of experiment 3 of the present invention. Detailed Implementation

[0039] To make the objectives, technical solutions, and advantages of this invention clearer, the invention is described below with reference to specific embodiments, but it should not be construed as limiting the scope of the invention to the following embodiments.

[0040] Example 1

[0041] A traditional Chinese medicine composition for treating coccidiosis in chickens is prepared from the following raw materials in parts by weight: 10 parts of *Gynostemma pentaphyllum*, 6 parts of germinated barley, 20 parts of *Toona sinensis* leaves, 6 parts of lotus seeds, 10 parts of *Hedyotis diffusa*, 6 parts of *Dendrobium nobile*, 20 parts of *Polygonum hydropiper*, 10 parts of *Thalictrum sylvestris*, and 5 parts of licorice. This traditional Chinese medicine composition for treating coccidiosis in chickens is prepared according to the following steps:

[0042] Step 1: Weigh out the following raw materials according to the specified weight proportions: 10 parts of Venus simonii, 6 parts of germinated barley, 20 parts of Toona sinensis leaves, 6 parts of lotus seeds, 10 parts of Water lily, 6 parts of Dendrobium, 20 parts of Fireweed, 10 parts of Thalictrum lucidum, and 5 parts of licorice; wash, dry, cut into sections, crush and pass through a No. 2 sieve, mix evenly, put into an extraction tank, add an appropriate amount of water to submerge the herbs by 4 cm, and soak for 1 hour;

[0043] The second step involves heating the Chinese medicinal materials from the first step to 50°C, adding 0.3% cellulase, adjusting the pH to 5, and hydrolyzing for 4 hours while stirring continuously.

[0044] The third step is to add water at a ratio of 10 times the total weight of all the herbs after the enzymatic hydrolysis is completed, bring to a boil, and then simmer for 1.5 hours.

[0045] The fourth step is to filter the Chinese medicine from the third step, and then add water equal to nine times the total weight of the medicinal materials to the filter residue and decoct for 1 hour.

[0046] Fifth step: Filter the Chinese medicine from the fourth step, then add water equal to 8 times the total weight of the medicinal materials and decoct for 1 hour.

[0047] Step 6: Combine the decoctions from the three decoctions, concentrate them under reduced pressure to a relative density of about 1.1, and spray dry to obtain material 1; then freeze-dry the enzymatically hydrolyzed residue under vacuum, pulverize it through an 80-mesh sieve to obtain material 2; mix material 1 and material 2 evenly, sterilize, and package to obtain the traditional Chinese medicine compound preparation of the present invention.

[0048] Example 2

[0049] The difference between this embodiment and Embodiment 1 lies in the raw materials (by weight): 15 parts of Venus simonii, 10 parts of germinated rice, 25 parts of Toona sinensis leaves, 10 parts of lotus seeds, 15 parts of water lily, 10 parts of Dendrobium, 23 parts of fire vine, 15 parts of Thalictrum lucidum, and 6 parts of licorice.

[0050] For specific preparation methods, please refer to Example 1.

[0051] Example 3

[0052] The difference between this embodiment and Embodiment 1 lies in the raw materials (by weight): 15 parts of Venus simonii, 10 parts of germinated rice, 30 parts of Toona sinensis leaves, 10 parts of lotus seeds, 15 parts of Water lily, 10 parts of Dendrobium, 25 parts of Fire Seedling, 15 parts of Thalictrum lucidum, and 6 parts of licorice.

[0053] For specific preparation methods, please refer to Example 1.

[0054] Example 4

[0055] The difference between this embodiment and Embodiment 1 lies in the raw materials (by weight): 15 parts of Venus simonii, 10 parts of germinated rice, 20 parts of Toona sinensis leaves, 10 parts of lotus seeds, 15 parts of Water lily, 10 parts of Dendrobium, 25 parts of Fire vine, 15 parts of Thalictrum lucidum, and 6 parts of licorice.

[0056] For specific preparation methods, please refer to Example 1.

[0057] Example 5

[0058] The difference between this embodiment and Embodiment 1 lies in the raw materials (by weight): 12 parts of Venus simonii, 8 parts of germinated rice, 30 parts of Toona sinensis leaves, 8 parts of lotus seeds, 12 parts of Water lily, 8 parts of Dendrobium, 25 parts of Fire Seedling, 12 parts of Thalictrum lucidum, and 5 parts of licorice.

[0059] For the specific preparation method, please refer to Example 1.

[0060] Comparative Example 1

[0061] This embodiment is a comparative example of a traditional Chinese medicine composition for treating coccidiosis in chickens. The difference between this comparative example and the embodiment is in the raw materials (by weight): 8 parts of *Gynostemma pentaphyllum*, 20 parts of germinated barley, 15 parts of *Toona sinensis* leaves, 15 parts of lotus seeds, 10 parts of *Hedyotis diffusa*, 10 parts of *Dendrobium nobile*, 10 parts of *Hypericum perforatum*, 20 parts of *Thalictrum sylvestris*, and 6 parts of licorice.

[0062] For the specific preparation method, please refer to Example 1.

[0063] Comparative Example 2

[0064] This example is another comparative example of a traditional Chinese medicine composition for treating coccidiosis in chickens, without the addition of Toona sinensis leaves and Water Flos. The difference between this comparative example and Example 3 is in the raw materials (by weight): 15 parts of Venus simonii, 10 parts of germinated rice, 10 parts of lotus seeds, 10 parts of Dendrobium, 25 parts of Ficus pumila, 15 parts of Thalictrum lucidum, and 6 parts of Glycyrrhiza uralensis.

[0065] For the specific preparation method, please refer to Example 1.

[0066] Comparative Example 3

[0067] This embodiment is another comparative example of a traditional Chinese medicine composition for treating coccidiosis in chickens. The difference between this comparative example and the embodiment lies in the raw materials (by weight parts):

[0068] 15 parts germinated rice, 30 parts toon leaves, 15 parts water lily, 15 parts dendrobium, 25 parts fire vine, 30 parts thalia sylvestris, and 6 parts licorice.

[0069] For the specific preparation method, please refer to Example 1.

[0070] Comparative Example 4

[0071] This embodiment is another comparative example of a traditional Chinese medicine composition for treating coccidiosis in chickens. Dendrobium is replaced with Astragalus membranaceus, and Venus simonii and Pteris vittata are not added. The difference between this comparative example and the embodiment is in the raw materials (by weight): 10 parts germinated rice, 30 parts Toona sinensis leaves, 10 parts lotus seeds, 15 parts Water lily, 10 parts Astragalus membranaceus, 15 parts Thalictrum lucidum, and 6 parts licorice.

[0072] Astragalus; Flavor and nature: warm; sweet. Channels entered: lung and spleen. Functions and effects: tonifies qi and strengthens the exterior, promotes tissue regeneration and healing of sores. Indications: spontaneous sweating due to weakness, chronic diarrhea, rectal prolapse, uterine prolapse, chronic nephritis, edema due to weakness, chronic ulcers, and slow-healing sores.

[0073] For the specific preparation method, please refer to Example 1.

[0074] Experimental Example 1: Verification of the efficacy of the traditional Chinese medicine composition of the present invention in the prevention and treatment of coccidiosis in artificially infected chickens.

[0075] 1. Experimental Materials

[0076] experimental animals

[0077] One-day-old male chicks were purchased from Beijing Huadu Yukou Poultry Industry Co., Ltd.

[0078] 1.2 The test insect species, E. tenella oocysts, was identified and preserved by the testing center of the Biotechnology Research Institute of Beijing Shengtai Technology Co., Ltd.

[0079] 2. Test Methods

[0080] 2.1 Insect treatment

[0081] The preserved Eimeria tenella oocyst fluid was centrifuged (3500 rpm, 10 min), the supernatant was discarded, and the precipitate was washed twice with water. The final wash with purified water was used to prepare an oocyst solution of a specific concentration. The final concentration was determined by McMaster plate counting to be 3.5 × 10⁻⁶. 4 Cells / ml. Virus challenge model was established at 3.5 × 10⁻⁶. 4 The virus was administered via gavage at a concentration of 1 ml per animal.

[0082] 2.2 Experimental grouping and treatment

[0083] Male chicks aged 1 day were raised to 14 days old. Weak and stunted chicks were removed, and 360 chicks were selected and divided into 12 experimental groups of 30 chicks each, based on the principle of uniform weight. Prophylactic administration of the drug was started on the day of grouping by mixing it into the feed. On the third day after grouping, coccidioidomyelitis was administered via gavage. The control group was given diclazuril granules (Shengtai (Inner Mongolia) Technology Co., Ltd.) at a dosage of 0.02% per kilogram of feed. Experimental groups 1-5 were administered the drugs described in Examples 1, 2, 3, 4, and 5 of this invention, respectively. Comparative groups 1-4 were administered the drugs described in Comparative Examples 1, 2, 3, and 4 of this invention, respectively. The grouping and treatment methods are shown in Table 1.

[0084] Table 1. Experimental grouping and treatment methods

[0085]

[0086] 2.3 Observation of experimental indicators

[0087] 2.3.1 Symptoms and necropsy findings

[0088] After the chickens were challenged with the virus, their mental state and fecal condition were observed. Dead chickens were dissected to observe cecal lesions.

[0089] 2.3.2 Blood in stool score

[0090] Fecal scoring was performed according to the method of Suo Xun (1997). On the 5th day after infection, the proportion of bloody stool was observed, photographed and recorded, and bloody stool was scored: For Eimeria tenella infection, within a given time range of 12-24 hours, 0 points indicated 100% bloodless stool, +1 point indicated 25% bloody stool, +2 points indicated 50% bloody stool, +3 points indicated 75% bloody stool, and +4 points indicated 100% bloody stool.

[0091] 2.3.3 Oocyte value

[0092] Seven days after the challenge, feces excreted within one day in each group were randomly sampled at multiple points. The McMaster counting method was used to count fecal oocysts to determine the number of oocysts per gram of feces in each group. The oocyst value was then calculated based on Table 2.

[0093] Oocyst ratio (%) = Number of oocysts per gram of feces in each group / Number of oocysts per gram of feces in the challenge group × 100%.

[0094] Table 2 Criteria for Determining Oocyte Bud Value

[0095]

[0096] 2.3.4 Relative weight gain rate

[0097] One day before the start of the experiment (grouping day) and on the 9th day of the experiment (slaughter day), each chicken in each group was weighed on an empty stomach, and the relative weight gain rate was calculated.

[0098] Relative weight gain rate = (average weight gain in the infected group / average weight gain in the healthy control group) × 100%.

[0099] 2.3.5 Survival rate

[0100] During the experiment, the number of surviving chickens in each group was counted, and the survival rate was calculated.

[0101] Survival rate = (Number of surviving chickens in each group / Total number of chickens in each group) × 100%.

[0102] 2.3.6 Cecal Lesion Values

[0103] The cecum was dissected, and cecal lesions were scored according to the method of Johnson and Reid (1970), and lesion values ​​were calculated.

[0104] Lesion score = (Total lesion score of the whole group / Total number of chickens in the group) × 10

[0105] Table 3 Scoring criteria for cecal lesions

[0106]

[0107] Note: Chickens that die after infection should be weighed and necropsyd immediately to determine the cause of death; any chicken that dies from coccidiosis will be scored 4 points for lesions.

[0108] 2.3.7 ACI Index Calculation

[0109] The anticoccidial ACI index was calculated according to the method of Merck & Co., Inc., and the calculation method is: ACI = (relative weight gain rate + survival rate) - (lesion value + oocyst value).

[0110] Judgment criteria: ACI ≥ 180 is considered sensitive, 160 ≤ ACI < 180 is considered good, 120 ≤ ACI < 160 is considered poor, and ACI < 120 is considered invalid.

[0111] 3. Experimental Results

[0112] Table 4. Statistical analysis of drug resistance to coccidiosis in each experimental group

[0113] Group Relative weight gain rate (%) Survival rate (%) ovarian cyst value Cecal lesion value ACI Blood in stool score Health Group 100 100 0 0 200 0 Model group 72.36 86.67 40 31.33 87.7 3 Chemical drug control group 91.2 100 5 5.33 180.87 0 <![CDATA[Experimental group 1 > 89.3 100 5 7.33 176.97 0 <![CDATA[Experimental group 2 > 89.89 100 5 6.33 178.56 0 <![CDATA[Experimental group 3 > 90.1 100 5 6.00 179.10 0 <![CDATA[Experimental group 4 > 88.66 100 5 6.67 176.99 0 <![CDATA[Experimental group 5 > 89.23 100 5 7.00 177.23 0 <![CDATA[Control group 1 > 86.23 100 10 15.33 160.90 1 <![CDATA[Control group 2 > 84.81 93.33 20 16.67 141.47 2 <![CDATA[Control group 3 > 85.63 100 10 17.00 158.63 2 <![CDATA[Control group 4 > 84.33 90 20 19.00 135.33 3

[0114] As shown in Table 4, the anticoccidial index (ACI) of experimental groups 1-5 in this experiment was higher than that of the model group and control groups 1-4, and close to that of the chemical drug control group (180.87). Among them, experimental group 3 (179.10) was 91.4 higher than that of the model group (87.7). The blood stool scores of experimental groups 1-5 were lower than those of the model group and control groups 1-4. The above clinical trial results show that the traditional Chinese medicine composition prepared in this invention has a significant anticoccidial effect in chickens and can effectively prevent and treat fecal abnormalities caused by coccidiosis.

[0115] Experimental Example 2: Clinical efficacy verification of the traditional Chinese medicine composition of the present invention in treating cecal coccidiosis.

[0116] Coccidiosis is common in poultry farming, especially in free-range chickens. This experiment was conducted at a poultry farm in Nanning, Guangxi, with a flock of over 20,000 chickens. The affected flock was 32 days old and free-range. Preliminary diagnosis based on on-site observation and necropsy was acute cecal coccidiosis infection. Clinical symptoms: bloody stools and feed droppings were observed on-site. Necropsy findings: cecal hemorrhage was found upon necropsy. Preliminary clinical diagnosis: outbreak of cecal coccidiosis. The experiment was randomly divided into four groups: an infection control group, a drug-treated control group, experimental group 1, and experimental group 2, with 5,000 chickens in each group. The drug treatment regimen and recorded data are shown in the table.

[0117] Table 5: Clinical treatment regimens and observation records for cecal coccidiosis

[0118]

[0119] Clinical trials have shown that the traditional Chinese medicine composition prepared in this invention can significantly improve cecal lesions, eliminate bloody stools, and reduce the number of deaths in chickens due to coccidiosis infection, indicating that the traditional Chinese medicine composition prepared in this invention has a good anti-coccidiosis effect in chickens.

[0120] Experimental Example 3: Clinical efficacy verification of the traditional Chinese medicine composition of the present invention in treating coccidiosis of the small intestine.

[0121] This clinical trial was conducted at a poultry farm in Yulin with a population of over 10,000 chickens. The affected flock consisted of chickens aged 76 days, free-range, exhibiting clinical symptoms including tomato-shaped droppings, feed-based droppings, loose feces, poor feed intake, and indigestion. The preliminary clinical diagnosis was coccidiosis of the small intestine. The chickens were randomly divided into four groups: an infection control group, a drug control group, experimental group 1, and experimental group 2, with 2,500 chickens in each group. The treatment regimen and recorded data are shown in the table.

[0122] Table 6: Clinical treatment regimens and observation records for small intestinal coccidiosis

[0123]

[0124] Clinical trials have shown that the traditional Chinese medicine composition prepared in this invention can effectively solve problems such as tomato-shaped droppings, unformed droppings, poor feed intake, and poor digestion in chickens caused by coccidiosis of the small intestine. This indicates that the traditional Chinese medicine composition prepared in this invention has a good anti-coccidiosis effect in chickens.

[0125] The herbal composition of this invention is based on the principles of syndrome differentiation and treatment in traditional Chinese veterinary medicine. It is rationally formulated, and all the herbs fully exert their pharmacological effects. When used together, they can achieve synergistic effects. It has the effects of stopping diarrhea and killing parasites, clearing heat and drying dampness, and tonifying the middle and replenishing qi. In addition, the herbal composition of this invention is prepared from pure Chinese medicinal materials, which has a greater advantage in combating coccidiosis in chickens and has a more obvious curative effect. It is expected to replace the use of traditional antibiotics in clinical practice.

[0126] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it.

Claims

1. A traditional Chinese medicine composition for treating coccidiosis in chickens, characterized in that, It is made from the following ingredients in parts by weight: 12-18 parts of Venus regia, 6-13 parts of germinated barley, 25-32 parts of Toona sinensis leaves, 8-13 parts of lotus seeds, 12-18 parts of water lily, 8-13 parts of Dendrobium, 22-28 parts of fire vine, 12-18 parts of Thalictrum lucidum, and 5-8 parts of licorice.

2. The traditional Chinese medicine composition for treating coccidiosis in chickens according to claim 1, characterized in that, It is made from the following ingredients in parts by weight: 15 parts of Venus regia, 10 parts of germinated rice, 30 parts of Toona sinensis leaves, 10 parts of lotus seeds, 15 parts of water lily, 10 parts of Dendrobium, 25 parts of fire vine, 15 parts of Thalictrum lucidum, and 6 parts of licorice.

3. A traditional Chinese medicine composition for treating coccidiosis in chickens, characterized in that, It includes the traditional Chinese medicine composition as described in claim 1 or 2 and pharmaceutically acceptable excipients.

4. The traditional Chinese medicine composition for treating coccidiosis in chickens according to claim 3, characterized in that, The dosage forms of the traditional Chinese medicine composition are powder, decoction, oral liquid, granules, pills, and tablets.

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