A Chinese medicine composition for nasal anti-inflammation and sterilization and its application

Abstract

The present invention relates to a Chinese medicine composition for nasal anti-inflammatory and sterilization and its application, and relates to the technical field of Chinese medicine. The preparation method of the Chinese medicine composition for nasal anti-inflammatory and sterilization comprises the following steps: under a pressurized state, tangerine peel, mulberry and Alpinia oxyphylla are added to an ammonia solution for soaking treatment, and then filtered to obtain a mixture; the mixture is added to water for reflux extraction, and the obtained extract is concentrated to obtain an extract; the extract is added to an acidic aqueous solution for stirring, and then an organic solvent is added for extraction to obtain an organic layer; the organic layer is concentrated and dried to obtain the Chinese medicine composition. The Chinese medicine composition provided by the present invention has excellent preventive and therapeutic effects on rats with acute bacterial infection rhinitis model, not only has significant anti-inflammatory and sterilization effects, but also can promote the repair of damaged nasal mucosa, making up for the shortcomings of existing nasal anti-inflammatory and sterilization products, and has great application prospects in the preparation of products for treating rhinitis.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicines, and in particular to a traditional Chinese medicine composition for nasal cavity anti-inflammation and sterilization and application thereof. Background Art

[0002] Rhinitis is one of the most common nasal diseases. Patients often experience symptoms such as nasal congestion, runny nose, headache, memory loss, and lack of energy, which seriously affect their daily life, work, and study. As air quality declines due to air pollution, rhinitis symptoms caused by pollen, indoor and outdoor dust, bacteria, animal fur, etc. are becoming more and more common. In severe cases, it can cause lesions such as congestion, swelling, exudation, hyperplasia, atrophy, or necrosis of the nasal mucosa.

[0003] At present, there are many types of products reported in the prior art for nasal anti-inflammatory and sterilization, such as intranasal glucocorticoids for the treatment of chronic rhinitis and oxymetazoline hydrochloride spray for improving the nasal congestion symptoms of allergic rhinitis. Although existing products for the treatment of rhinitis have good anti-inflammatory and sterilization effects, they generally do not have obvious nasal mucosal repair effects, which leads to problems such as easy recurrence of rhinitis patients after stopping medication. Summary of the invention

[0004] In order to solve the above problems, the present invention provides a Chinese medicine composition for nasal anti-inflammation and sterilization and its application.

[0005] In a first aspect, the present invention provides a Chinese medicine composition for nasal anti-inflammation and sterilization, and the preparation method of the Chinese medicine composition for nasal anti-inflammation and sterilization comprises the following steps:

[0006] Under pressure, tangerine peel, mulberry and Alpinia oxyphylla kernel are added into an ammonia solution for soaking, and then filtered and dried to obtain a mixture;

[0007] The mixed material is added into water for reflux extraction, and the obtained extract is concentrated to obtain a solid;

[0008] The solid is added into water, and then stirred under acidic conditions to obtain a mixed solution;

[0009] An organic solvent is added to the mixed solution for extraction, and then the obtained organic layer is concentrated and dried to obtain the traditional Chinese medicine composition.

[0010] Further, under pressure, the steps of adding tangerine peel, mulberry and Alpinia oxyphylla to an ammonia solution for soaking, followed by filtering and drying to obtain a mixture include the following processes:

[0011] Under an external pressure of 10-20 MPa, tangerine peel, mulberry and Alpinia oxyphylla kernel are added into an ammonia solution with a weight percentage of 10-25% and soaked at room temperature for 1-5 minutes, and then filtered and naturally dried to obtain the mixture.

[0012] Furthermore, the external pressure is 15 MPa, the weight percentage of the ammonia solution is 20%, and the time of the room temperature immersion treatment is 35 minutes.

[0013] Furthermore, the working parameters of the reflux extraction include: the reflux extraction temperature is 100-110° C., the reflux extraction time is 1-2 hours, and the weight ratio of the mixture to the water is 1:(8-15).

[0014] Furthermore, the solid is added into water, and then stirred under acidic conditions to obtain a mixed solution, which comprises the following process:

[0015] The solid is added into water to dissolve, and then concentrated hydrochloric acid is added to adjust the pH of the system to 3.2-4.5, and stirred for 10-30 minutes to obtain a mixed solution.

[0016] Furthermore, the pH of the system is 3.6-4.0.

[0017] Furthermore, the organic solvent includes at least one of dichloromethane, ethyl acetate and chloroform.

[0018] Furthermore, the weight ratio of the tangerine peel, the mulberry and the Alpinia oxyphylla kernel is (40-50):(7-13):(1-5).

[0019] In a second aspect, the present invention provides a use of a traditional Chinese medicine composition for nasal anti-inflammatory and sterilization as described in any one of the first aspects in the preparation of a product for treating rhinitis and / or a product for promoting the repair of damaged nasal mucosa.

[0020] Furthermore, the product is in the form of at least one of a liquid lotion, a solid ointment and an aerosol spray.

[0021] The above technical solution provided by the present invention has at least the following advantages compared with the prior art:

[0022] The present invention provides a Chinese medicine composition for nasal anti-inflammatory and sterilization. The present invention uses tangerine peel, mulberry and Alpinia oxyphylla as raw materials, and prepares a Chinese medicine composition for nasal anti-inflammatory and sterilization by a combined treatment method of pre-treating the raw materials with water by soaking in an ammonia solution and acid washing the products after water extraction. The Chinese medicine composition has excellent preventive and therapeutic effects on rats with acute bacterial infection rhinitis model, not only has significant anti-inflammatory and sterilization effects, but also can promote the repair of damaged nasal mucosa, making up for the shortcomings of existing nasal anti-inflammatory and sterilization products, and has great application prospects in the preparation of products for treating rhinitis. DETAILED DESCRIPTION

[0023] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be clearly and completely described in combination with the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0024] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or prepared by existing methods.

[0025] In a first aspect, the present invention provides a Chinese medicine composition for nasal anti-inflammation and sterilization, and the preparation method of the Chinese medicine composition for nasal anti-inflammation and sterilization comprises the following steps:

[0026] Under pressure, tangerine peel, mulberry and Alpinia oxyphylla kernel are added into an ammonia solution for soaking, and then filtered and dried to obtain a mixture;

[0027] The mixed material is added into water for reflux extraction, and the obtained extract is concentrated to obtain a solid;

[0028] The solid is added into water, and then stirred under acidic conditions to obtain a mixed solution;

[0029] An organic solvent is added to the mixed solution for extraction, and then the obtained organic layer is concentrated and dried to obtain the traditional Chinese medicine composition.

[0030] The present invention provides a Chinese medicine composition for nasal anti-inflammatory and sterilization. The present invention uses tangerine peel, mulberry and Alpinia oxyphylla as raw materials, and prepares a Chinese medicine composition for nasal anti-inflammatory and sterilization by a combined treatment method of pre-treating the raw materials with water by soaking in an ammonia solution and acid washing the products after water extraction. The Chinese medicine composition has excellent preventive and therapeutic effects on rats with acute bacterial infection rhinitis model, not only has significant anti-inflammatory and sterilization effects, but also can promote the repair of damaged nasal mucosa, making up for the shortcomings of existing nasal anti-inflammatory and sterilization products, and has great application prospects in the preparation of products for treating rhinitis.

[0031] In the present invention, the tangerine peel is the dried outer peel of the citrus plant tangerine and its cultivars of the Rutaceae family, and has the effects of eliminating phlegm, promoting qi, relaxing the stomach, and dispersing nodules.

[0032] In the present invention, mulberry is the fruit of the plant of the genus Morus in the Moraceae family, and has the effects of lowering blood lipids and blood pressure, strengthening the spleen and nourishing the stomach, and darkening hair and beautifying the skin.

[0033] In the present invention, the Alpinia oxyphylla kernel is the fruit of the plant Alpinia oxyphylla of the Zingiberaceae family, and has the effects of warming the spleen, stopping diarrhea, and controlling salivation, warming the kidney, reducing urine, and consolidating semen.

[0034] In some specific embodiments, the step of adding tangerine peel, mulberry and Alpinia oxyphylla to an ammonia solution for soaking under pressure, followed by filtering and drying to obtain a mixture comprises the following process:

[0035] Under an external pressure of 10-20 MPa, tangerine peel, mulberry and Alpinia oxyphylla kernel are added into an ammonia solution with a weight percentage of 10-25% and soaked at room temperature for 1-5 minutes, and then filtered and naturally dried to obtain the mixture.

[0036] In some specific embodiments, during the soaking treatment, the amount of ammonia solution added is sufficient to ensure that the tangerine peel, mulberries and Alpinia oxyphylla kernels are completely immersed.

[0037] In some specific embodiments, the external pressure is 15 MPa, the weight percentage of the ammonia solution is 20%, and the time of the room temperature immersion treatment is 35 minutes.

[0038] In some specific embodiments, the working parameters of the reflux extraction include: the reflux extraction temperature is 100-110° C., the reflux extraction time is 1-2 hours, and the weight ratio of the mixture to the water is 1:(8-15).

[0039] In some specific embodiments, the step of adding the solid to water and then stirring under acidic conditions to obtain a mixed solution comprises the following process:

[0040] The solid is added into water to dissolve, and then concentrated hydrochloric acid is added to adjust the pH of the system to 3.2-4.5, and stirred for 10-30 minutes to obtain a mixed solution.

[0041] In some specific embodiments, the pH of the system is 3.6-4.0.

[0042] In some specific embodiments, the organic solvent includes at least one of dichloromethane, ethyl acetate and chloroform; preferably chloroform.

[0043] In some specific embodiments, the weight ratio of the tangerine peel, the mulberry and the Alpinia oxyphylla kernel is (40-50):(7-13):(1-5); preferably 47:11:4.

[0044] It should be noted that, unless otherwise specified or described, the components and raw materials involved in the traditional Chinese medicine composition for nasal anti-inflammatory and sterilization provided by the present invention can be directly commercially available products or homemade using existing preparation processes; at the same time, the process steps and parameters involved can be carried out according to the preparation process disclosed in the prior art or using existing equipment unless otherwise specified or described, and the present invention document will not describe them one by one.

[0045] In a second aspect, the present invention provides a use of a traditional Chinese medicine composition for nasal anti-inflammatory and sterilization as described in any one of the first aspects in the preparation of a product for treating rhinitis and / or a product for promoting the repair of damaged nasal mucosa.

[0046] In some specific embodiments, the product is in the form of at least one of a liquid lotion, a solid ointment, and an aerosol spray.

[0047] The present invention will be further described below in conjunction with specific examples. It should be understood that these examples are intended only to illustrate the present invention and are not intended to limit the scope of the present invention. The experimental methods in the following examples that do not specify specific conditions are usually measured according to national standards. If there is no corresponding national standard, then the conditions recommended by the manufacturer are followed.

[0048] Example 1

[0049] This example provides a Chinese medicine composition for nasal anti-inflammatory and sterilization, and the preparation method of the Chinese medicine composition for nasal anti-inflammatory and sterilization comprises the following steps:

[0050] Step (1): under an external pressure of 15 Mpa, adding tangerine peel, mulberry and Alpinia oxyphylla in a weight ratio of 47:11:4 to a 20% by weight ammonia solution for soaking at room temperature for 3 minutes, then filtering and naturally drying to obtain the mixture;

[0051] Step (2): adding the mixture obtained in step (1) into water for reflux extraction, the reflux extraction temperature is 105° C., the reflux extraction time is 1.5 hours, the weight ratio of the mixture to the water is 1:10, and the obtained extract is concentrated to obtain a solid;

[0052] Step (3): adding the solid obtained in step (2) into water to dissolve, then adding concentrated hydrochloric acid to adjust the pH of the system to 3.8, stirring for 20 minutes, and obtaining a mixed solution;

[0053] Step (4): adding chloroform to the mixed solution obtained in step (3) for extraction, separating the liquids, and then concentrating and vacuum drying the obtained organic layer to obtain the traditional Chinese medicine composition.

[0054] Example 2

[0055] This example provides a Chinese medicine composition for nasal anti-inflammatory and sterilization, and the preparation method of the Chinese medicine composition for nasal anti-inflammatory and sterilization comprises the following steps:

[0056] Step (1): under an external pressure of 20 MPa, adding tangerine peel, mulberry and Alpinia oxyphylla kernel in a weight ratio of 40:7:1 to a 10% by weight ammonia solution for soaking at room temperature for 1 minute, then filtering and naturally drying to obtain the mixture;

[0057] Step (2): adding the mixture obtained in step (1) into water for reflux extraction, the reflux extraction temperature is 105° C., the reflux extraction time is 1.5 hours, the weight ratio of the mixture to the water is 1:10, and the obtained extract is concentrated to obtain a solid;

[0058] Step (3): adding the solid obtained in step (2) into water to dissolve, then adding concentrated hydrochloric acid to adjust the pH of the system to 3.2, stirring for 20 minutes, and obtaining a mixed solution;

[0059] Step (4): adding chloroform to the mixed solution obtained in step (3) for extraction, separating the liquids, and then concentrating and vacuum drying the obtained organic layer to obtain the traditional Chinese medicine composition.

[0060] Example 3

[0061] This example provides a Chinese medicine composition for nasal anti-inflammatory and sterilization, and the preparation method of the Chinese medicine composition for nasal anti-inflammatory and sterilization comprises the following steps:

[0062] Step (1): under an external pressure of 10 Mpa, adding tangerine peel, mulberry and Alpinia oxyphylla kernel in a weight ratio of 50:13:5 to a 25% by weight ammonia solution for soaking at room temperature for 5 minutes, then filtering and naturally drying to obtain the mixture;

[0063] Step (2): adding the mixture obtained in step (1) into water for reflux extraction, the reflux extraction temperature is 105° C., the reflux extraction time is 1.5 hours, the weight ratio of the mixture to the water is 1:10, and the obtained extract is concentrated to obtain a solid;

[0064] Step (3): adding the solid obtained in step (2) into water to dissolve it, then adding concentrated hydrochloric acid to adjust the pH of the system to 4.5, stirring for 20 minutes, and obtaining a mixed solution;

[0065] Step (4): adding chloroform to the mixed solution obtained in step (3) for extraction, separating the liquids, and then concentrating and vacuum drying the obtained organic layer to obtain the traditional Chinese medicine composition.

[0066] Comparative Example 1

[0067] This example provides a Chinese medicine composition for nasal anti-inflammatory and sterilization and a preparation method thereof, which is different from Example 1 only in that the raw water is not pre-treated by soaking in ammonia solution in advance and the product after water extraction is not acid-washed; the remaining steps and parameters are the same.

[0068] The preparation method of the above-mentioned Chinese medicine composition for nasal anti-inflammatory and sterilization comprises the following steps:

[0069] Step (1): mixing tangerine peel, mulberry and Alpinia oxyphylla kernel in a weight ratio of 47:11:4 to obtain a mixture;

[0070] Step (2): adding the mixture obtained in step (1) into water for reflux extraction, the reflux extraction temperature is 105° C., the reflux extraction time is 1.5 hours, the weight ratio of the mixture to the water is 1:10, and the obtained extract is concentrated to obtain the Chinese medicine composition.

[0071] Comparative Example 2

[0072] This example provides a Chinese medicine composition for nasal anti-inflammatory and sterilization and a preparation method thereof, which is different from Example 1 only in that the raw water is not pre-treated by soaking in ammonia solution in advance; the remaining steps and parameters are the same.

[0073] The preparation method of the above-mentioned Chinese medicine composition for nasal anti-inflammatory and sterilization comprises the following steps:

[0074] Step (1): mixing tangerine peel, mulberry and Alpinia oxyphylla kernel in a weight ratio of 47:11:4 to obtain a mixture;

[0075] Step (2): adding the mixture obtained in step (1) into water for reflux extraction, the reflux extraction temperature is 105° C., the reflux extraction time is 1.5 hours, the weight ratio of the mixture to the water is 1:10, and the obtained extract is concentrated to obtain a solid;

[0076] Step (3): adding the solid obtained in step (2) into water to dissolve, then adding concentrated hydrochloric acid to adjust the pH of the system to 3.8, stirring for 20 minutes, and obtaining a mixed solution;

[0077] Step (4): adding chloroform to the mixed solution obtained in step (3) for extraction, separating the liquids, and then concentrating and vacuum drying the obtained organic layer to obtain the traditional Chinese medicine composition.

[0078] Comparative Example 3

[0079] This example provides a Chinese medicine composition for nasal anti-inflammatory and sterilization and a preparation method thereof, which is different from Example 1 only in that the product after water extraction is not subjected to acid washing; the remaining steps and parameters are the same.

[0080] The preparation method of the above-mentioned Chinese medicine composition for nasal anti-inflammatory and sterilization comprises the following steps:

[0081] Step (1): under an external pressure of 15 Mpa, adding tangerine peel, mulberry and Alpinia oxyphylla in a weight ratio of 47:11:4 to a 20% by weight ammonia solution for soaking at room temperature for 3 minutes, then filtering and naturally drying to obtain the mixture;

[0082] Step (2): adding the mixture obtained in step (1) into water for reflux extraction, the reflux extraction temperature is 105° C., the reflux extraction time is 1.5 hours, the weight ratio of the mixture to the water is 1:10, and the obtained extract is concentrated to obtain a solid;

[0083] Step (3): adding the solid obtained in step (2) into water to dissolve it, then adding chloroform to extract it, separating the liquids, and finally concentrating and vacuum drying the obtained organic layer to obtain the traditional Chinese medicine composition.

[0084] Comparative Example 4

[0085] This example provides a Chinese medicine composition for nasal anti-inflammatory and sterilization and a preparation method thereof, which is different from Example 1 only in that the weight ratio of tangerine peel, mulberry and Alpinia oxyphylla is adjusted to 11:47:4; the remaining steps and parameters are the same.

[0086] Test Case

[0087] In this example, the efficacy of the Chinese medicine compositions provided in Examples 1 to 3 and Comparative Examples 1 to 4 was tested.

[0088] Experimental animals: 90 SD rats, half male and half female, were divided into a normal control group (no modeling, no medication), a model control group (modeling, no medication) and 7 experimental groups (modeling, medication, oral administration of 1.0 g kg -1 ), 10 in each group.

[0089] Experimental method: According to the existing method, the rat rhinitis model was prepared by nasal drops of Staphylococcus aureus yeast venom solution (10 mL of bacterial solution contains 1 g of dry yeast). Before the experiment, the experimental group was given preventive medication for 3 days; except for the normal control group, the rats in the other groups were given 120 μL·d of Staphylococcus aureus yeast venom solution. -1Nasal drops were continuously administered for 4 days to establish the model. At the same time, the corresponding dose of the drug solution was administered by gavage. The normal control group and the model control group were gavaged with the same amount of water. The drug was continued for 3 days after the last nasal drops to establish the model. Blood was collected from the femoral artery 0.5 hours after the last administration, and the serum was separated. The level of C-reactive protein (CRP) in the serum was detected by immunoturbidimetry using an automatic biochemical analyzer. The animals were then killed, and the nasal mucosa and bronchial tissues were taken, fixed with 5% formalin, and stained with HE to observe the pathological morphological changes of the nasal mucosal epithelium and bronchial epithelial tissues. The experimental data were processed and analyzed using software, and the test results were expressed as mean ± standard deviation. The test results are shown in Table 1.

[0090] Table 1

[0091]

[0092]

[0093] It can be seen from Table 1 that: compared with the comparative example, the Chinese medicine composition provided by the embodiment of the present invention has a better therapeutic effect on rats with acute bacterial infection rhinitis model, and has a significant effect of promoting the repair of damaged nasal mucosa, and can be used to prepare products for treating rhinitis, and / or, products that promote the repair of damaged nasal mucosa.

[0094] In summary, the present invention provides a Chinese medicine composition for nasal anti-inflammatory and sterilization. The present invention uses tangerine peel, mulberry and Alpinia oxyphylla as raw materials, and prepares a Chinese medicine composition for nasal anti-inflammatory and sterilization by a combined treatment method of pre-treating the raw materials with water by soaking in ammonia solution and acid washing the products after water extraction. The Chinese medicine composition has excellent preventive and therapeutic effects on rats with acute bacterial infection rhinitis model, not only has significant anti-inflammatory and sterilization effects, but also can promote the repair of damaged nasal mucosa, making up for the shortcomings of existing nasal anti-inflammatory and sterilization products, and has great application prospects in the preparation of products for treating rhinitis.

[0095] The foregoing is merely a specific embodiment of the present invention, which enables those skilled in the art to understand or implement the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features claimed herein.

Claims

1. A Chinese medicine composition for nasal anti-inflammatory and sterilization, characterized in that: The preparation method of the Chinese medicine composition for nasal anti-inflammatory and sterilization comprises the following steps: Under an external pressure of 10-20 MPa, the tangerine peel, mulberry and Alpinia oxyphylla kernel are added to an ammonia solution with a weight percentage of 10%-25% and soaked at room temperature for 1-5 minutes, and then filtered and naturally dried to obtain a mixture; The mixed material is added into water for reflux extraction, and the obtained extract is concentrated to obtain a solid; The solid was added into water to dissolve, and then concentrated hydrochloric acid was added to adjust the pH of the system to 3.2-4.5, and stirred for 10-30 minutes to obtain a mixed solution; Adding an organic solvent to the mixed solution for extraction, and then concentrating and drying the obtained organic layer to obtain the traditional Chinese medicine composition; The working parameters of the reflux extraction include: the reflux extraction temperature is 100-110°C, the reflux extraction time is 1-2 hours, and the weight ratio of the mixture to the water is 1:(8-15); The organic solvent comprises at least one of dichloromethane, ethyl acetate and chloroform; The weight ratio of the tangerine peel, the mulberry and the Alpinia oxyphylla kernel is (40-50): (7-13): (1-5).

2. The Chinese medicine composition for nasal anti-inflammatory and sterilization according to claim 1, characterized in that: The external pressure is 15 MPa, the weight percentage of the ammonia solution is 20%, and the time of the room temperature immersion treatment is 35 minutes.

3. The Chinese medicine composition for nasal anti-inflammatory and sterilization according to claim 1, characterized in that: The pH of the system is 3.6-4.

0.

4. Use of a Chinese medicine composition for nasal anti-inflammatory and sterilization according to any one of claims 1 to 3 in the preparation of a product for treating rhinitis or promoting the repair of damaged nasal mucosa.

5. The use according to claim 4, characterized in that: The product is in the form of at least one of a liquid lotion, a solid ointment and an aerosol spray.

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