Intelligent management of bacterial strain preservation system

Abstract

The present invention discloses an intelligently managed bacterial strain preservation system. The system includes: an abnormal sample identification and processing module, a bacterial strain transfer-in module, a bacterial strain transfer-out module, a bacterial strain preservation detection module, and a freezer early warning module. The present invention controls a mechanical arm to transfer the corresponding bacterial strain preservation box of the normal bacterial strain sample on the initial input transmission track to an idle position in the freezer according to bacterial strain sample information. The freezer controls the mechanical arm to transfer the corresponding bacterial strain preservation box of the bacterial strain sample from the corresponding position of the freezer to the bacterial strain receiving position according to the bacterial strain transfer-out command information. The freezer area corresponds to the bacterial strain category one by one. The freezer detects the bacterial strain samples with a variation trend in the freezer in real time. The variation refers to an obvious change in the colony state and the death of the bacterial strain. The bacterial strain samples with a variation trend in the freezer are also processed in time, thereby completing the bacterial strain sample transfer-in, transfer-out and preservation operations, and then intelligently managing the bacterial strain sample preservation, solving the problem of inconvenient bacterial strain preservation management.

Description

Technical Field

[0001] The present invention relates to the technical field of bacterial strain preservation, and in particular to a bacterial strain preservation system with intelligent management. Background Art

[0002] With the continuous development of science and technology, more and more new technologies are applied to the preservation of bacterial strains. Bacterial strains have the characteristic of easy mutation. During the preservation process, the metabolic activity of the bacterial strains is kept in an inactive or relatively static state to slow down the decay rate of the bacterial strains, preserve the original excellent traits of the bacterial strains, and prevent contamination by other bacteria. For the preservation of bacterial strains, the use of freezing cabinets can control the temperature and humidity at a constant temperature, protect the strains from contamination by other bacteria, and effectively extend the storage time of the strains.

[0003] At present, there are many aspects of research on strain preservation. For example, Chinese invention patent CN116426376A discloses an intelligent strain culture medium filling and preservation device, in which a strain processing component is provided on one side of the raw material processing component, a culture medium diversion component is provided at one end of the raw material processing component, a storage component is provided at the middle bottom of the culture medium diversion component, a buffer platform is provided between the culture medium diversion component and the raw material processing component, a lateral processing component is provided at the distal axially symmetrical position of the raw material processing component, and a filling component is provided on the side of the raw material processing component and the lateral processing component. The selection and integration of equipment such as liquid processing systems, sorting equipment and sensors using automated technical solutions can realize automated strain culture medium filling and storage. The liquid processing system can perform liquid processing and filling without stopping, thereby improving production efficiency and sorting. The equipment can quickly and accurately classify and separate liquids, thereby further improving production efficiency; for example, Chinese invention patent CN115512773A discloses a food strain resource library management system that assists in food strain management and sharing, through a strain library and a strain resource management subsystem and a sharing platform subsystem respectively connected to the strain library; the strain resource management subsystem includes: a visual management module, a container management module, a strain management module, and an application management module, which are used by administrators to approve the entry and exit of strains; the sharing platform subsystem includes: a strain search module, which is used to search for strain sample information in the strain library by name and view and trace; a strain statistics module, which is used to count the strains in the strain library and view the strain sample information; a strain application module, which is used for the public to apply for strains; and a platform service module, which is used to introduce service information.

[0004] However, current strain preservation equipment is generally backward and is usually stored in household -20℃ refrigerators or industrial -80℃ ultra-low temperature refrigerators. These strain preservation devices are not specially designed for strain preservation. The equipment temperature is unstable, humidity control is difficult, and the location of the strains cannot be accurately determined. It is impossible to identify abnormal samples or accurately transfer strain samples in and out, resulting in inconvenience in strain preservation and management.

[0005] Therefore, in summary, the prior art has the problem of inconvenient strain preservation and management. Summary of the invention

[0006] The embodiment of the present application solves the problem of inconvenient strain preservation management in the prior art by providing an intelligently managed strain preservation system, and realizes intelligent management of strain sample preservation.

[0007] The embodiment of the present application provides an intelligently managed strain preservation system, including: an abnormal sample identification and processing module, a strain transfer-in module, a strain transfer-out module, a strain preservation detection module and a freezer warning module; wherein the abnormal sample identification and processing module is used to identify and process the strain sample to be preserved, obtain initial input abnormal sample information and strain sample information, and control a mechanical arm to process the initial input abnormal sample according to the initial input abnormal sample information and strain sample information; the strain transfer-in module is used to control the mechanical arm to transfer the corresponding strain preservation box of the normal strain sample on the initial input conveying track to an empty position in the freezer according to the strain sample information; the strain transfer-out module is used to control the mechanical arm to transfer the corresponding strain preservation box of the normal strain sample on the initial input conveying track to an empty position in the freezer according to the strain sample information; The freezer controls the robotic arm to transfer the corresponding strain preservation box of the strain sample from the corresponding position of the freezer to the strain receiving place according to the strain transfer command information sent by the user; the strain preservation detection module is used to detect the strain samples with mutation trends in the freezer in real time, and is also used to deal with the strain samples with mutation trends in the freezer in time, and analyze the strain preservation quality index of all categories of strains, and the strain preservation quality index is used to judge the preservation capacity of the freezer for different categories of strains; the freezer early warning module is used to issue an early warning according to the strain preservation quality index. When the strain preservation quality index is lower than the first threshold value, the freezer issues an early warning, indicating that there is a problem with the strain preservation quality of the freezer.

[0008] Furthermore, the specific method for identifying and processing the strain samples to be stored is as follows: when the track trigger sensor on the initial input conveying track identifies the presence of a strain preservation box, the strain sample identification device identifies the barcode on the strain preservation box to obtain the strain sample information; the strain sample identification device detects the strain samples in all the strain preservation boxes on the initial input conveying track, and if the strain sample in the strain preservation box is identified as an abnormal strain sample, it is recorded as the initial input abnormal sample, otherwise it is recorded as a normal strain sample; when the strain sample identification device detects that the strain sample on the initial input conveying track is an abnormal strain sample, the strain sample identification device sends the initial input abnormal sample information and the strain sample information of the strain preservation box to the freezer.

[0009] Furthermore, the specific operating method for processing the initial input abnormal sample is: the freezer forwards the received initial input abnormal sample information and the strain sample information of the strain preservation box to the robotic arm, and sends a second control instruction to the robotic arm, and the second control instruction is used to control the robotic arm to grab the corresponding strain preservation box of the initial input abnormal sample to the corresponding detection and transmission track to re-detect the abnormal sample; when the robotic arm receives the second control instruction, the robotic arm grabs the corresponding strain preservation box of the initial input abnormal sample according to the initial input abnormal sample information, and places the abnormal sample on the corresponding detection and transmission track according to the strain sample information of the strain preservation box.

[0010] Furthermore, the specific method of controlling the robot arm to transfer the corresponding strain preservation boxes of the normal strain samples on the initial input conveying track to the vacant positions in the freezer is as follows: after the freezer analyzes the corresponding area information of the strain sample in the freezer based on the strain category information in the acquired strain sample information, it sends the transfer information to the robot arm and sends a first control instruction to the robot arm, wherein the first control instruction is used to control the robot arm to transfer the corresponding strain preservation boxes of all strain samples to the forked conveying track that can reach the corresponding area in the freezer; when the robot arm receives the first control instruction, the robot arm transfers the corresponding strain preservation boxes of the normal strain samples on the initial input conveying track to the vacant positions in the freezer based on the transfer information. The strain preservation box containing the normal strain sample is initially input onto the conveying track and is transferred to the forked conveying track that can reach the corresponding freezer area; when the strain preservation box is transferred to the end of the forked conveying track, the forked conveying track sends a transfer information to the freezer; the freezer sends the transfer information to the robotic arm, and sends a third control instruction to the robotic arm, wherein the third control instruction is used to control the robotic arm to grab the corresponding strain preservation box of the normal strain sample to an empty position in the freezer; when the robotic arm receives the third control instruction, the robotic arm grabs the strain preservation box of the normal strain sample to an empty position in the corresponding area of ​​the freezer according to the transfer information.

[0011] Furthermore, the strain transfer-out module includes a position determination unit and a transfer-out unit; the position determination unit is used for the freezer to determine the position information of the strain preservation box where the strain sample to be transferred out is located in the freezer according to the strain transfer-out command information, and then the freezer sends the transfer-out information to the robotic arm, wherein the strain transfer-out command information includes the type of strain to be taken out, the number of the strain sample to be taken out, and the number of strains to be taken out; the transfer-out unit is used for the robotic arm to grab the strain preservation box from the freezer to the output conveying track and transmit it to the strain transfer-out receiving point according to the position information of the strain preservation box where the strain sample is located in the transfer-out information.

[0012] Furthermore, the specific method for determining the position information of the strain preservation box where the strain sample to be transferred out is located in the freezer is: the freezer analyzes the position information of the strain preservation box where the strain sample to be transferred out is located in the freezer based on the received strain transfer command information, and the strain transfer command information includes taking out strain samples of specified strain categories and the number of strains is greater than one, and also includes taking out a strain sample with a specified strain sample number; when the strain transfer command information is to take out strain samples of specified strain categories and the number of strains is greater than one, the freezer area where the transfer operation needs to be performed is determined according to the transferred strain category, and the strain sample position in the freezer area closest to the freezer exit is used as the determined position; when the strain transfer command information is to take out a strain sample with a specified strain sample number, according to the storage position information of the specified strain sample number stored in the freezer, it is used as the determined position.

[0013] Furthermore, the strain preservation detection module includes a preservation condition determination unit, a variation sample detection unit, and a strain preservation quality index analysis unit; the preservation condition determination unit is used to divide the freezer into N areas, and there are N types of strain samples, the freezer areas correspond to the strain categories one by one, and the temperature and humidity set in the freezer areas are the optimal preservation temperature and optimal preservation humidity corresponding to the strain categories, respectively; the variation sample detection unit is used to detect in real time whether the strain samples in the freezer have a variation trend. When it is detected that the strain samples in the freezer have a variation trend, the strain samples with the variation trend are processed in time to obtain variation sample information, and the variation sample information includes the strain sample information, the time of entry into the cabinet, and the time of appearance; the strain preservation quality index analysis unit is used to analyze the strain preservation quality index of each strain category according to the variation sample information.

[0014] Furthermore, the specific method for timely processing of bacterial species samples with a tendency to mutate is as follows: the freezer monitors in real time whether the bacterial species samples being frozen and stored have a tendency to mutate; if there is no tendency to mutate, the bacterial species samples being frozen and stored continue to be monitored; if there is a tendency to mutate, the bacterial species samples with a tendency to mutate are recorded as frozen abnormal bacterial species samples, and the freezer sends the frozen abnormal sample information to the robotic arm, and sends a fourth control command to the robotic arm, and the fourth control command is used to control the robotic arm to grab the corresponding bacterial species storage box of the frozen abnormal sample to the detection transmission track; when the robotic arm receives the fourth control command, the robotic arm grabs the frozen abnormal bacterial species sample according to the frozen abnormal sample information. The strain preservation box should be put on the detection conveyor track; the detection conveyor track will convey the frozen abnormal sample to the detection room of the corresponding strain category for re-testing of the strain sample; if it is determined that the strain sample has mutated, the strain sample will be recorded as a mutant sample, and the strain will be discarded according to user needs or the sample will be changed into a normal strain sample in the detection room through certain means, new strain sample information will be generated, and a bar code generated by the corresponding strain sample information encoding will be printed on the new strain preservation box, and the strain preservation box will be placed on the initial input conveyor track for transfer; if it is determined that the strain sample has not mutated, the strain sample will be returned to the original position of the freezer for refrozen storage.

[0015] Furthermore, the specific analysis method of the strain preservation quality index is: sequentially obtain the appearance time and entry time of variant samples under a strain category; based on the appearance time and entry time of the variant samples, count the preservation time of the variant samples; obtain the number and appearance ratio of variant samples during variant sample detection; based on the preservation time and the number of appearance of variant samples, calculate the average preservation time of all variant samples under a strain category; obtain the theoretical shelf life of the strain category, compare it with the average preservation time, and obtain the shelf life comparison ratio; comprehensively consider the appearance ratio of variant samples and the shelf life comparison ratio, and use the normalization method to construct a strain preservation quality index calculation formula; and obtain the strain preservation quality index according to the strain preservation quality index calculation formula.

[0016] Furthermore, the calculation formula of the strain preservation quality index is:

[0017]

[0018] In the formula, YC i is the strain preservation quality index of the i-th strain, A1 is the weight value of the shelf life comparison ratio in the strain preservation quality index, A2 is the weight value of the occurrence ratio of variant samples in the strain preservation quality index, i is the strain category number, i=1,2,3,…,N, N is the total number of strain categories, are the appearance time and entry time of the jth variant sample of the i-th strain, respectively. j is the variant sample number under the i-th strain category, j = 1, 2, 3, ..., j0, j0 is the total number of variant samples, RG i is the theoretical shelf life of the i-th strain, YS i is the occurrence ratio of variant samples of the i-th bacterial species.

[0019] One or more technical solutions provided in the embodiments of the present application have at least the following technical effects or advantages:

[0020] 1. By controlling the robot arm according to the strain sample information, the corresponding strain preservation box of the normal strain sample on the initial input transmission track is transferred to the vacant position in the freezer. The freezer controls the robot arm to transfer the corresponding strain preservation box of the strain sample from the corresponding position of the freezer to the strain receiving position according to the strain transfer command information sent by the user. The freezer area corresponds to the strain category one by one. The freezer detects the strain samples with mutation trends in the freezer in real time, and is also used to process the strain samples with mutation trends in the freezer in time, thereby completing the strain sample transfer-in, transfer-out and storage operations, thereby realizing intelligent management of strain sample storage, and effectively solving the problem of inconvenient strain storage management in the prior art.

[0021] 2. By combining the appearance time, cabinet entry time, appearance quantity, appearance ratio and theoretical shelf life of variant samples under a bacterial species category, the bacterial species preservation quality index of the species is analyzed, and then the preservation capacity of the entire freezer for a single category of bacterial species is evaluated.

[0022] The received initial input abnormal sample information and the strain sample information of the strain preservation box are forwarded to the robotic arm through the freezer, and a second control instruction is sent to the robotic arm. The robotic arm grabs the corresponding strain preservation box of the initial input abnormal sample to the detection and transmission track according to the initial input abnormal sample information to re-detect the abnormal sample, thereby ensuring that there are no abnormal samples on the initial input transmission track, and further achieving the goal of no abnormal samples being transferred to the freezer for frozen storage. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 A schematic diagram of the structure of the intelligently managed bacterial strain storage system provided in an embodiment of the present application;

[0024] Figure 2 A schematic diagram of the structure of the strain transfer module provided in the embodiment of the present application;

[0025] Figure 3 This is a schematic diagram of the structure of the strain preservation detection module provided in the embodiment of the present application. DETAILED DESCRIPTION

[0026] The embodiment of the present application solves the problem of inconvenient strain preservation management in the prior art by providing an intelligently managed strain preservation system. The system controls a robotic arm according to strain sample information to transfer the strain preservation box corresponding to the normal strain sample on the initial input transmission track to an empty position in the freezer. The freezer controls the robotic arm to transfer the strain preservation box corresponding to the strain sample from the corresponding position of the freezer to the strain receiving position according to the strain transfer command information sent by the user. The freezer area corresponds to the strain category one by one. The freezer detects strain samples with mutation trends in the freezer in real time and processes the strain samples with mutation trends in the freezer in a timely manner, thereby completing the strain sample transfer-in, transfer-out and preservation operations and realizing intelligent management of strain sample preservation.

[0027] The technical solution in the embodiment of the present application is to solve the problem of inconvenient storage and management of the above-mentioned strains, and the overall idea is as follows:

[0028] By obtaining the strain sample information such as the strain sample number, strain name, strain category name, etc. of the strain sample, the strain sample information is encoded to generate a corresponding barcode, the generated barcode is printed on a strain preservation box, the corresponding strain sample is placed in the strain preservation box, and all the pre-treated strain preservation boxes are placed on the strain initial input transmission track; when the track trigger sensor on the strain initial transmission track recognizes that there is a strain preservation box, the strain sample identification device identifies the barcode on the strain preservation box to obtain the strain sample information, and the strain sample identification device sends the strain sample information to the freezer, and the freezer detects the strain sample according to the strain sample information. The strain category information is analyzed to obtain the corresponding storage area information of the strain sample freezer, and the freezer sends the transfer information to the robotic arm; a strain sample identification device is installed on the initial input conveying track, which can identify abnormal samples of the strain samples in the strain preservation box. When the strain sample identification device identifies the abnormal sample, the robotic arm will grab the strain preservation box with the abnormal sample to the corresponding sample detection conveying track, and re-test the strain sample to ensure that there is no abnormal sample on the initial input conveying track; for the strain preservation boxes of other normal samples on the initial input conveying track, the strain preservation boxes are re-tested according to the freezer area information analyzed previously. The storage box is transferred to the strain bifurcated conveying track that can reach the corresponding freezer area. When the strain storage box is transferred to the end of the strain bifurcated conveying track, the robotic arm places the corresponding strain storage box in the vacant position of the corresponding freezer area. The freezer records the time when the strain sample enters the cabinet and the strain sample storage location information to complete the strain transfer. The freezer has N areas, and each area of ​​the freezer stores strain samples of corresponding categories. At the same time, each area has the best storage temperature and humidity for each strain sample. The freezer detects strain samples with mutation trends in the freezer in real time. If there are no strain samples with mutation trends, the freezer continues to monitor. For the bacterial sample in the freezer, if there is a bacterial sample with a mutation trend, the bacterial sample with the mutation trend will be transferred out of the freezer to the detection conveyor track for re-testing the bacterial sample; the bacterial preservation quality index of all bacterial categories is analyzed, and the preservation capacity of the freezer for different types of bacterial species is evaluated according to the bacterial preservation quality index of all bacterial categories; when the freezer receives the bacterial transfer command, according to the bacterial sample storage position information, the robotic arm grabs the bacterial preservation box from the corresponding position of the freezer to the output conveyor track, and transmits the bacterial preservation box to the bacterial receiving place through the output track to complete the bacterial transfer, so as to achieve intelligent management of bacterial sample storage.

[0029] In order to better understand the above technical solution, the above technical solution will be described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0030] like Figure 1As shown, it is a structural schematic diagram of the intelligently managed strain preservation system provided in the embodiment of the present application. The intelligently managed strain preservation system provided in the embodiment of the present application includes: an abnormal sample identification and processing module, a strain transfer-in module, a strain transfer-out module, a strain preservation detection module and a freezer early warning module; wherein the abnormal sample identification and processing module is used to identify and process the strain sample to be preserved, obtain the initial input abnormal sample information and the strain sample information, and control the mechanical arm to process the initial input abnormal sample according to the initial input abnormal sample information and the strain sample information; the strain transfer-in module is used to control the mechanical arm to transfer the corresponding strain preservation box of the normal strain sample on the initial input transmission track into the freezer according to the strain sample information. a free position of the strain transfer module, which is used for the freezer to control the robotic arm to transfer the corresponding strain storage box of the strain sample from the corresponding position of the freezer to the strain receiving place according to the strain transfer command information sent by the user; a strain preservation detection module, which is used to detect the strain samples with mutation trends in the freezer in real time, and is also used to deal with the strain samples with mutation trends in the freezer in time, and analyze the strain preservation quality index of all categories of strains, and the strain preservation quality index is used to judge the preservation capacity of the freezer for different categories of strains; a freezer early warning module, which is used to issue an early warning according to the strain preservation quality index. When the strain preservation quality index is lower than the first threshold value, the freezer issues an early warning, indicating that there is a problem with the strain preservation quality of the freezer.

[0031] In this embodiment, the strain sample information includes the strain sample number, strain name, strain category name, strain source, strain storage temperature, strain storage humidity, etc.; the initial input abnormal sample information is the information transmitted to the freezer when the initial input is detected to have an abnormal sample on the initial input transmission track. After the freezer receives the information, the freezer sends the information to the mechanical arm, and the mechanical arm grabs the abnormal sample to the detection transmission track according to the initial input abnormal sample information; the lower the strain preservation quality index, the worse the preservation ability of the freezer for different types of strains; when the strain preservation quality index is lower than the first threshold, the freezer issues an early warning, and the staff inspects and repairs the corresponding area of ​​the freezer according to which type of strain preservation quality index is lower than the first threshold. The freezer early warning module also includes a temperature control alarm unit, which is used for the freezer to issue an early warning when the freezer deviates from the optimal set temperature by 10% and the time lasts for 2 hours or more, so as to ensure the survival rate of the preserved strains and repair the freezer, and if necessary, perform the recovery and subculture of all frozen strains.

[0032] Furthermore, the specific method for identifying and processing the strain samples to be stored is as follows: when the track trigger sensor on the initial input conveying track identifies the presence of a strain preservation box, the strain sample identification device identifies the barcode on the strain preservation box to obtain the strain sample information; the strain sample identification device detects the strain samples in all the strain preservation boxes on the initial input conveying track, if the strain sample in the strain preservation box is identified as an abnormal strain sample, it is recorded as the initial input abnormal sample, otherwise it is recorded as a normal strain sample; when the strain sample identification device detects that the strain sample on the initial input conveying track is an abnormal strain sample, the strain sample identification device sends the initial input abnormal sample information and the strain sample information of the strain preservation box to the freezer.

[0033] In this embodiment, the freezer, the initial input conveyor track, the strain output track and the robotic arm are all equipped with information receiving and output devices, which can receive and output various information for completing the abnormal sample processing of the strain, the transfer of strain samples in and out; obtaining the strain sample information, encoding the strain sample information to generate a corresponding barcode, printing the generated barcode on the strain preservation box, and placing the strain sample in the corresponding strain preservation box; placing the strain preservation boxes containing the strain samples on the initial input conveyor track. Since the initial input conveyor track is equipped with an infrared scanning device, the barcode on the strain preservation box can be identified to obtain the strain sample information.

[0034] Furthermore, the specific operating method for processing the initial input abnormal sample is as follows: the freezer forwards the received initial input abnormal sample information and the strain sample information of the strain preservation box to the robotic arm, and sends a second control instruction to the robotic arm, and the second control instruction is used to control the robotic arm to grab the corresponding strain preservation box of the initial input abnormal sample to the corresponding detection and transmission track to re-detect the abnormal sample; when the robotic arm receives the second control instruction, the robotic arm grabs the corresponding strain preservation box of the initial input abnormal sample according to the initial input abnormal sample information, and places the abnormal sample on the corresponding detection and transmission track according to the strain sample information of the strain preservation box.

[0035] In this embodiment, the strain sample identification device can identify whether the strain sample is an abnormal strain sample according to the colony state of the strain sample. When the strain sample identification device identifies that the strain state of the strain sample to be analyzed is different from the known colony state of the normal strain sample, and the colony morphology changes from round to irregular, the edges are not neat, the shape is irregular, and the colony color and colony size are inconsistent, the current strain sample is likely to be an abnormal sample, and normal colony state pictures of all categories of strain samples are obtained. By taking the colony state picture of the current strain sample and comparing it with the normal colony state picture of the corresponding category, the abnormal sample is identified. Perform comparative analysis and identification, vectorize the colony state image of the current strain sample and the normal colony state image, analyze the cosine similarity of the two images, and also obtain the similarity of the two images through SSIM (structural similarity algorithm), histogram, hash function and other algorithms. When the similarity of the two images is lower than the first threshold, the strain sample is recorded as the initial input abnormal sample, and the initial input abnormal sample information is sent to the robot arm, which grabs the abnormal sample to the detection transmission track according to the abnormal sample information. Since the appearance of the strain sample has changed, it is not necessarily possible to conclude that the strain sample has mutated, but only that the strain sample has changed. The strain sample has a tendency to mutate, and needs to be retested. After using microscope, strain identification and drug sensitivity, gene sequencing and other technologies to detect whether the abnormal sample has really mutated, the strain sample retest information is sent to the freezer for further processing; the freezer obtains the strain sample retest information, and the freezer determines whether the strain has mutated based on the strain sample retest information; if it is determined that the strain sample has mutated, the strain is discarded according to the user's needs, or in the test room, the abnormal sample is changed into a normal strain sample by certain means, and new strain sample information is generated and printed in a new strain storage box. The barcode corresponding to the strain sample information encoding is generated, and the strain preservation box is placed on the initial input conveying track for transfer; if no mutation occurs, the strain sample is put back on the initial input conveying track; a strain sample identification device is installed at the entrance of the initial input conveying track, and the strain sample identification device can detect whether there are abnormal samples in all strain preservation boxes on the initial input conveying track, and the abnormal samples on the initial input track are grabbed by the mechanical arm to the corresponding detection conveying track to ensure that there are no abnormal samples on the initial input conveying track, thereby avoiding abnormal samples from being stored in the freezer.

[0036] Furthermore, the specific method of controlling the robot arm to transfer the corresponding strain preservation boxes of the normal strain samples on the initial input conveying track to the vacant positions in the freezer is as follows: after the freezer analyzes the corresponding area information of the strain sample in the freezer based on the strain category information in the acquired strain sample information, it sends the transfer information to the robot arm and sends a first control instruction to the robot arm, the first control instruction is used to control the robot arm to transfer the corresponding strain preservation boxes of all strain samples to the forked conveying track that can reach the corresponding area in the freezer; when the robot arm receives the first control instruction, the robot arm transfers the initial strain preservation boxes to the vacant positions in the freezer based on the transfer information. The strain preservation box containing the normal strain sample on the input conveying track is transferred to the forked conveying track that can reach the corresponding freezer area; when the strain preservation box is transferred to the end of the forked conveying track, the forked conveying track sends a transfer information to the freezer; the freezer sends the transfer information to the robotic arm, and sends a third control instruction to the robotic arm, and the third control instruction is used to control the robotic arm to grab the corresponding strain preservation box of the normal strain sample to an empty position in the freezer; when the robotic arm receives the third control instruction, the robotic arm grabs the strain preservation box of the normal strain sample to an empty position in the corresponding area of ​​the freezer according to the transfer information.

[0037] In this embodiment, the freezer records the entry time and storage location information of the bacterial sample number.

[0038] Further, such as Figure 2 As shown, it is a structural schematic diagram of the strain transfer-out module provided in an embodiment of the present application, and the strain transfer-out module includes a position determination unit and a transfer-out unit; the position determination unit is used for the freezer to determine the position information of the strain preservation box where the strain sample to be transferred out is located in the freezer according to the strain transfer-out command information, and then the freezer sends the transfer-out information to the robotic arm, and the strain transfer-out command information includes the type of strain to be taken out, the number of the strain sample to be taken out, and the number of strains to be taken out; the transfer-out unit is used for the robotic arm to grab the strain preservation box from the freezer and transfer it to the output conveying track to the strain transfer-out receiving point according to the position information of the strain preservation box where the strain sample is located in the transfer-out information.

[0039] In this embodiment, when the freezer receives the bacterial strain transfer-out command information sent by the user, the position information of the bacterial strain storage box containing the bacterial strain sample to be transferred out in the freezer is determined.

[0040] Furthermore, a specific method for determining the position information of the strain preservation box containing the strain sample to be transferred out in the freezer is as follows: the freezer analyzes the position information of the strain preservation box containing the strain sample to be transferred out in the freezer based on the received strain transfer-out command information, the strain transfer-out command information including taking out strain samples of a specified strain category and a strain number greater than one, and also including taking out a strain sample with a specified strain sample number; when the strain transfer-out command information is to take out strain samples of a specified strain category and a strain number greater than one, the freezer area where the transfer-out operation needs to be performed is determined based on the transferred strain category, and the strain sample position in the freezer area closest to the freezer exit is used as the determined position; when the strain transfer-out command information is to take out a strain sample with a specified strain sample number, based on the storage position information of the specified strain sample number stored in the freezer, it is used as the determined position.

[0041] In this embodiment, when the strain transfer command information is to take out a strain sample of a specified strain category and the number of strains is greater than one, the strain sample position closest to the freezer exit in the freezer area is used as the determined position, and the corresponding number of strain samples are transferred out of the freezer from the determined position; when a strain sample with a specified strain sample number is taken out, the storage position information of the specified strain sample number stored in the freezer is used as the determined position, and a strain sample with the corresponding strain sample number is transferred out of the freezer from the determined position and ends; dividing the strain transfer command information into two categories after analysis is conducive to better organization and management of the strain sample transfer process. When a large number of strain samples of the same category need to be transferred out, the strain sample samples of the specified category and strain number can be selected to be taken out, and when a strain sample with a specified strain sample number needs to be taken out, the strain sample with the specified strain sample number can be directly taken out, thereby improving the work efficiency of strain sample transfer.

[0042] Further, such as Figure 3 As shown, it is a structural schematic diagram of the strain preservation detection module provided in an embodiment of the present application, the strain preservation detection module includes a preservation condition determination unit, a variation sample detection unit, and a strain preservation quality index analysis unit; the preservation condition determination unit is used to divide the freezer into N areas, and there are N types of strain samples, the freezer areas correspond to the strain categories one by one, and the temperature and humidity set in the freezer areas are the optimal preservation temperature and the optimal preservation humidity corresponding to the strain categories, respectively; the variation sample detection unit is used to detect in real time whether the strain samples in the freezer have a variation trend. When it is detected that the strain samples in the freezer have a variation trend, the strain samples with the variation trend are processed in time to obtain the variation sample information, and the variation sample information includes the strain sample information, the cabinet entry time and the appearance time; the strain preservation quality index analysis unit is used to analyze the strain preservation quality index of each strain category according to the variation sample information.

[0043] In this embodiment, the freezer is divided into N areas, and there are N types of bacterial samples. The freezer areas correspond to the bacterial types one by one. The temperature and humidity set in the freezer areas are the optimal storage temperature and optimal storage humidity corresponding to the bacterial types, respectively. Storing different types of bacterial samples separately can effectively prevent cross-contamination and mixing. Each area has a specific storage temperature and humidity, which can provide different types of bacterial samples with the most suitable environmental conditions for their growth and survival, thereby better maintaining the purity and integrity of the bacterial samples, helping to prevent the metabolism and growth rate of the bacterial samples from being too fast or too slow, extending the shelf life of the bacterial samples, and reducing quality loss.

[0044] Furthermore, a specific method for timely processing of bacterial samples with a tendency to mutate is as follows: the freezer monitors in real time whether the bacterial samples being frozen and stored have a tendency to mutate; if there is no tendency to mutate, the bacterial samples being frozen and stored continue to be monitored; if there is a tendency to mutate, the bacterial samples with a tendency to mutate are recorded as abnormal frozen bacterial samples, and the freezer sends abnormal frozen sample information to the robot arm, and sends a fourth control command to the robot arm, the fourth control instruction is used to control the robot arm to grab the bacterial storage box corresponding to the abnormal frozen sample to the detection transmission track; when the robot arm receives the fourth control command, the robot arm grabs the abnormal frozen sample according to the abnormal frozen sample information. The corresponding strain storage box of the common strain sample is put onto the detection conveyor track; the detection conveyor track conveys the frozen abnormal sample to the detection room of the corresponding strain category for re-testing the strain sample; if it is determined that the strain sample has mutated, the strain is discarded according to the user's needs or the abnormal sample is changed into a normal strain sample in the detection room through certain means, new strain sample information is generated and a barcode generated by the corresponding strain sample information encoding is printed in the new strain storage box, and the strain storage box is placed on the initial input conveyor track for transfer; if it is determined that the strain sample has not mutated, the strain sample is returned to the original position of the freezer for refrozen storage.

[0045] In this embodiment, the abnormal frozen sample information is the information sent to the robot arm after the freezer detects that the strain sample being frozen has a tendency to mutate. The robot arm grabs the corresponding strain preservation box to the detection conveying track according to the abnormal frozen sample information and performs subsequent operations. The abnormal frozen sample information includes the location information of the strain preservation box of the abnormal frozen strain sample in the freezer area and the strain type information of the abnormal frozen strain sample. In order to preserve the strain sample and conduct subsequent inspections, a small opening can be left on the test tube cover. The small opening on the test tube cover can be used by the strain sample high-resolution identification device of the freezer to take pictures of the colony status of each strain sample, and oxygen can also be provided to enter the test tube to maintain the survival state of the colony. The test tube mouth is covered by a pore membrane. This material can filter out bacteria and other contaminants, and can also be transparent for the identification device to take high-definition pictures of the colony status. When designing and using the small opening on the test tube cover, ensure that the small opening is small enough to prevent external microorganisms and contaminants from entering the test tube. A strain sample high-resolution identification device is installed in each area and each layer of the freezer. , it is possible to take real-time pictures of the colony status of the bacterial sample as high-resolution pictures. These high-resolution pictures can check the colony status in more detail, compare and analyze the colony status picture of the current bacterial sample with the normal colony status picture of the same resolution, vectorize the colony status picture of the current bacterial sample and the normal colony status picture, and analyze the cosine similarity of the two pictures. In addition, the similarity of the two pictures can be obtained by algorithms such as SSIM (structural similarity algorithm), histogram, and hash function. When the similarity of the two pictures is lower than the second threshold, the bacterial sample has a mutation trend and is recorded as a frozen abnormal bacterial sample. The freezer sends the frozen abnormal sample information to the mechanical arm, and the mechanical arm grabs the abnormal sample to the detection transmission track according to the frozen abnormal sample information, and re-tests the bacterial sample to avoid freezing and storing frozen abnormal bacterial samples in the freezer; in the detection room, use microscopy, bacterial identification and drug sensitivity, gene sequencing and other technologies to detect whether the frozen abnormal sample is really mutated, and then proceed to the next step; if it is determined that the bacterial sample is mutated, the bacterial sample is called a mutant sample.

[0046] Furthermore, the specific analysis method of the strain preservation quality index is as follows: obtain the appearance time and entry time of variant samples under a strain category in turn; based on the appearance time and entry time of the variant samples, count the preservation time of the variant samples; obtain the number and appearance ratio of variant samples when detecting variant samples; based on the preservation time and the number of appearance of variant samples, calculate the average preservation time of all variant samples under a strain category; obtain the theoretical shelf life of the strain category, compare it with the average preservation time, and obtain the shelf life comparison ratio; comprehensively consider the appearance ratio of variant samples and the shelf life comparison ratio, and use the normalization method to construct a strain preservation quality index calculation formula; and obtain the strain preservation quality index based on the strain preservation quality index calculation formula.

[0047] In this embodiment, when the appearance of the colony state in the bacterial sample changes, the colony morphology changes from round to irregular, or the edges are uneven, the shape is irregular, the colony color is uneven, and the colony size is inconsistent, it indicates that the bacterial sample has a tendency to mutate, and the bacterial sample with a tendency to mutate is recorded as a frozen abnormal bacterial sample; however, further testing is required to determine whether the bacterial sample has mutated. If a mutation is determined through microscopic examination, bacterial identification and drug sensitivity, gene sequencing and other tests, the mutated bacterial sample is called a mutant sample.

[0048] Furthermore, the calculation formula of the strain preservation quality index is:

[0049]

[0050] In the formula, YC i is the strain preservation quality index of the i-th strain, A1 is the weight value of the shelf life comparison ratio in the strain preservation quality index, A2 is the weight value of the occurrence ratio of variant samples in the strain preservation quality index, i is the strain category number, i=1,2,3,…,N, N is the total number of strain categories, are the appearance time and entry time of the jth variant sample of the i-th strain, respectively. j is the variant sample number under the i-th strain category, j = 1, 2, 3, ..., j0, j0 is the total number of variant samples, RG i is the theoretical shelf life of the i-th strain, YS i is the occurrence ratio of variant samples of the i-th bacterial species.

[0051] In this embodiment, when the freezer is transferring bacterial samples in and out, changes and fluctuations in temperature and humidity will occur, because each time the freezer area door is opened, the indoor temperature and humidity will change in a short term. After fresh air enters the freezer, it needs to restore the low temperature state and the humidity state suitable for the preservation of bacterial samples. This process takes a certain amount of time to recover. Changes and fluctuations in the temperature and humidity of the freezer may cause the frozen preservation quality of the bacterial samples to decline, thereby affecting the survival stability of the bacterial samples. The preservation status of the overall bacterial species in the freezer can be obtained based on the average bacterial species preservation quality index, so as to avoid misjudging the overall preservation capacity of the freezer due to the poor preservation capacity of the freezer for a single type of bacterial species. The calculation formula of the average bacterial species preservation quality index is: Where YC is the average preservation quality index of the strain.

[0052] In summary, with the aid of the above-mentioned technical scheme of the present invention, the corresponding strain preservation box of the normal strain sample initially input on the conveying track is transferred to an empty position in the freezer by controlling the robotic arm according to the strain sample information. The freezer controls the robotic arm to transfer the corresponding strain preservation box of the strain sample from the corresponding position of the freezer to the strain receiving position according to the strain transfer-out command information sent by the user. The freezer area corresponds to the strain category one by one. The freezer detects the strain samples with mutation trends in the freezer in real time and processes the strain samples with mutation trends in the freezer in time, thereby completing the strain sample transfer-in, transfer-out and storage operations, and further realizing the intelligent management of strain sample storage.

[0053] Through the description of the above implementation methods, technical personnel in the relevant field can understand that for the convenience and simplicity of description, only the division of the above-mentioned functional modules is used as an example. In actual applications, the above-mentioned functions can be assigned to different functional modules as needed, that is, the internal structure of the specific device can be divided into different functional modules to complete all or part of the functions described above.

[0054] Those of ordinary skill in the art will appreciate that the modules and algorithm steps of each example described in conjunction with the embodiments disclosed herein can be implemented in electronic hardware, computer software, or a combination of the two. In order to clearly illustrate the interchangeability of hardware and software, the composition and steps of each example have been generally described in terms of function in the above description. Whether these functions are performed in hardware or software depends on the specific application and design constraints of the technical solution. Professional and technical personnel can use different methods to implement the described functions for each specific application, but such implementation should not be considered to be beyond the scope of this application.

[0055] In several embodiments covered by the present application, it should be understood that the disclosed systems, devices and methods can be implemented in other ways. For example, the division of modules is only a logical function division, and there may be other division methods in actual implementation, such as multiple modules or components can be combined or integrated into another system, or some features can be ignored or not executed. In addition, the mutual coupling or direct coupling or communication connection shown or discussed can be an indirect coupling or communication connection through some interfaces, devices or modules, or it can be an electrical, mechanical or other form of connection.

[0056] The modules described as separate components may or may not be physically separated, and the components displayed as modules may or may not be physical modules, that is, they may be located in one place or distributed on multiple network modules. Some or all of the modules may be selected according to actual needs to achieve the purpose of the embodiments of the present application.

[0057] In addition, each functional module in each embodiment of the present application can be implemented in the form of hardware or in the form of software functional modules. If these functional modules are implemented in the form of software functional modules and sold or used as independent products, they can be stored in a computer-readable storage medium. Based on such an understanding, the technical solution of the present application is essentially or part of the prior art that contributes to the prior art, or all or part of the technical solution can be embodied in the form of a computer program product, which includes one or more computer instructions. When loading and executing computer program instructions on a computer, the process or function according to the embodiment of the present application is generated in whole or in part. The computer can be a general-purpose computer, a special-purpose computer, a computer network, or other programmable devices. The computer program product is stored in a computer-readable storage medium, or transmitted from one computer-readable storage medium to another computer-readable storage medium, for example, a computer instruction can be transmitted from a website site, a computer, a server or a data center by wired (e.g., coaxial cable, optical fiber, digital subscriber line (DSL)) or wireless (e.g., infrared, wireless, microwave, etc.) mode to another website site, computer, server or data center. The computer-readable storage medium can be any available medium that a computer can access or a data storage device such as a server or a data center that includes one or more available media integrations. The available media may be magnetic media (eg, floppy disks, hard disks, magnetic tapes), optical media (eg, DVDs), or semiconductor media (eg, solid state drives (SSDs)).

[0058] The above contents are merely examples and explanations of the structure of the present invention. The technicians in this technical field may make various modifications or additions to the specific embodiments described or replace them in a similar manner. As long as they do not deviate from the structure of the invention or exceed the scope defined by the claims, they should all fall within the protection scope of the present invention.

Claims

1. Intelligent management of bacterial strain preservation system, characterized by: include: Abnormal sample identification and processing module, strain transfer-in module, strain transfer-out module, strain preservation detection module and freezer early warning module; The abnormal sample identification and processing module is used to identify and process the bacterial sample to be stored, obtain the initial input abnormal sample information and bacterial sample information, and control the mechanical arm to process the initial input abnormal sample according to the initial input abnormal sample information and bacterial sample information; The bacterial strain transfer module is used to control the mechanical arm to transfer the corresponding bacterial strain storage box of the normal bacterial strain sample on the initial input conveying track to an empty position in the freezer according to the bacterial strain sample information; The strain transfer module is used for the freezer to control the mechanical arm to transfer the corresponding strain storage box of the strain sample from the corresponding position of the freezer to the strain receiving place according to the strain transfer command information sent by the user; The bacterial strain preservation detection module is used to detect bacterial strain samples with mutation trends in the freezer in real time, and is also used to timely process bacterial strain samples with mutation trends in the freezer, and analyze the bacterial strain preservation quality index of all types of bacterial strains. The bacterial strain preservation quality index is used to judge the preservation capacity of the freezer for different types of bacterial strains; The freezer warning module is used to issue a warning according to the strain preservation quality index. When the strain preservation quality index is lower than a first threshold, the freezer issues a warning, indicating that there is a problem with the strain preservation quality of the freezer; The strain preservation detection module includes a preservation condition determination unit, a variant sample detection unit, and a strain preservation quality index analysis unit; The storage condition determination unit is used to divide the freezer into N areas, and there are N types of bacterial samples in total. The freezer areas correspond to the bacterial types one by one, and the temperature and humidity set in the freezer areas are the optimal storage temperature and optimal storage humidity corresponding to the bacterial types respectively; The variant sample detection unit is used to detect in real time whether the bacterial samples in the freezer have a variation trend. When it is detected that the bacterial samples in the freezer have a variation trend, the bacterial samples with the variation trend are processed in time to obtain the variant sample information, which includes the bacterial sample information, the time of entry into the freezer, and the time of appearance; The bacterial strain preservation quality index analysis unit is used to analyze the bacterial strain preservation quality index of each bacterial strain category according to the variant sample information; The specific analysis method of the strain preservation quality index is: Obtain the appearance time and cabinet entry time of variant samples under a bacterial species category in sequence; According to the appearance time and entry time of the variant samples, the storage time of the variant samples is counted; Obtain the number and ratio of variant samples during variant sample detection; According to the storage time and the number of variant samples, the average storage time of all variant samples under a bacterial species category was calculated; Obtain the theoretical shelf life of each fungus type, compare it with the average storage time, and obtain the shelf life comparison ratio; The normalization method was used to construct the calculation formula of the strain preservation quality index by combining the occurrence ratio of variant samples and the comparison ratio of shelf life. The strain preservation quality index was obtained according to the strain preservation quality index calculation formula.

2. The intelligent management bacterial strain storage system according to claim 1, characterized in that: The specific method for identifying and processing the bacterial species sample to be preserved is: When the track trigger sensor on the initial input conveying track recognizes that there is a strain preservation box, the strain sample identification device identifies the barcode on the strain preservation box to obtain the strain sample information; The strain sample identification device is used to detect the strain samples in all strain storage boxes on the initial input conveying track. If the strain sample in the strain storage box is identified as an abnormal strain sample, it will be recorded as the initial input abnormal sample, otherwise it will be recorded as a normal strain sample. When the strain sample identification device detects that the strain sample on the initial input conveying track is an abnormal strain sample, the strain sample identification device sends the initial input abnormal sample information and the strain sample information of the strain storage box to the freezer.

3. The intelligent management bacterial strain storage system according to claim 1, characterized in that: The specific operation method of the initial input abnormal sample processing is: The freezer forwards the received initial input abnormal sample information and the strain sample information of the strain storage box to the robotic arm, and sends a second control instruction to the robotic arm, wherein the second control instruction is used to control the robotic arm to grab the strain storage box corresponding to the initial input abnormal sample to the corresponding detection conveying track to re-detect the abnormal sample; When the robot arm receives the second control instruction, the robot arm grabs the corresponding strain preservation box of the initial input abnormal sample according to the initial input abnormal sample information, and places the abnormal sample on the corresponding detection and transmission track according to the strain sample information of the strain preservation box.

4. The intelligently managed bacterial strain preservation system according to claim 1, characterized in that: The specific method of controlling the mechanical arm to transfer the corresponding bacterial strain storage box of the normal bacterial strain sample initially input on the conveying track to the vacant position in the freezer is: The freezer analyzes the corresponding area information of the strain sample in the freezer according to the strain category information in the obtained strain sample information, sends transfer information to the robot arm, and sends a first control instruction to the robot arm, wherein the first control instruction is used to control the robot arm to transfer the corresponding strain storage boxes of all strain samples to the forked transmission track that can reach the corresponding area in the freezer; When the robot arm receives the first control instruction, the robot arm transfers the bacterial strain preservation box containing the normal bacterial strain sample on the initial input conveying track to the forked conveying track that can reach the corresponding freezer area according to the transfer information; When the culture storage box is transferred to the end of the forked conveying track, the forked conveying track sends the transfer information to the freezer; The freezer sends the transfer information to the robot arm, and sends a third control instruction to the robot arm, wherein the third control instruction is used to control the robot arm to grab the corresponding strain storage box of the normal strain sample to an empty position of the freezer; When the robot arm receives the third control instruction, the robot arm grabs the bacteria preservation box of the normal bacteria sample to an empty position in the corresponding area of ​​the freezer according to the transfer information.

5. The intelligently managed bacterial strain storage system according to claim 1, characterized in that: The strain transfer module includes a position determination unit and a transfer unit; The position determination unit is used for the freezer to determine the position information of the strain preservation box where the strain sample to be transferred out is located in the freezer according to the strain transfer command information, and then the freezer sends the transfer information to the robot arm, wherein the strain transfer command information includes the type of strain to be taken out, the number of the strain sample to be taken out, and the number of strains to be taken out; The transfer unit is used to grab the strain preservation box from the freezer and transfer it to the output conveying track to the strain transfer receiving point by a mechanical arm according to the position information of the strain preservation box containing the strain sample in the freezer in the transfer information.

6. The intelligent management bacterial strain storage system according to claim 5, characterized in that: The specific method for determining the position information of the strain storage box where the strain sample to be transferred out is located in the freezer is: The freezer analyzes the position information of the strain preservation box containing the strain sample to be transferred out in the freezer according to the received strain transfer command information, wherein the strain transfer command information includes taking out strain samples of a specified strain category and a strain number greater than one, and also includes taking out a strain sample of a specified strain sample number; When the bacterial strain transfer-out command information is to take out bacterial strain samples of a specified bacterial strain category and the number of bacterial strains is greater than one, the freezer area that needs to be transferred out is determined according to the transferred bacterial strain category, and the position of the bacterial strain sample in the freezer area closest to the freezer exit is used as the determined position; When the strain transfer out command information is to take out a strain sample with a specified strain sample number, the storage location information of the specified strain sample number stored in the freezer is used as the determined location.

7. The intelligently managed bacterial strain storage system according to claim 1, characterized in that: The specific method for timely processing of bacterial samples with a tendency to mutate is as follows: The freezer monitors in real time whether the bacterial samples being frozen and stored have any mutation trend; If there is no mutation trend, continue to monitor the strain samples that are being frozen; If there is a variation trend, the strain sample with the variation trend is recorded as a frozen abnormal strain sample, and the freezer sends the frozen abnormal sample information to the robot arm, and sends a fourth control command to the robot arm, and the fourth control command is used to control the robot arm to grab the corresponding strain storage box of the frozen abnormal sample to the detection conveying track; When the robot arm receives the fourth control command, the robot arm grabs the strain storage box corresponding to the frozen abnormal strain sample and puts it on the detection conveying track according to the frozen abnormal sample information; The abnormal frozen samples are transported to the testing room of the corresponding bacterial species by the testing conveyor track for retesting of the bacterial species; When the freezer obtains the retest information of the strain sample, the freezer determines whether the strain has mutated based on the retest information of the strain sample; If it is determined that the bacterial sample has mutated, the bacterial sample can be discarded according to user needs, or the abnormal sample can be converted into a normal bacterial sample in the testing room through certain means, new bacterial sample information is generated, and a barcode generated by the corresponding bacterial sample information encoding is printed in a new bacterial preservation box, and the bacterial preservation box is placed on the initial input transmission track for transfer. If it is determined that the bacterial sample has not mutated, the bacterial sample is returned to the original position in the freezer for refrozen preservation.

8. The intelligent management bacterial strain storage system according to claim 1, characterized in that: The calculation formula of the strain preservation quality index is: In the formula, YC i is the strain preservation quality index of the i-th strain, A1 is the weight value of the shelf life comparison ratio in the strain preservation quality index, A2 is the weight value of the occurrence ratio of variant samples in the strain preservation quality index, i is the strain category number, i=1,2,3,...,N, N is the total number of strain categories, are the appearance time and entry time of the jth variant sample of the i-th strain, respectively. j is the variant sample number under the i-th strain category, j=1,2,3,...,j0, j0 is the total number of variant samples, RG i is the theoretical shelf life of the i-th strain, YS i is the occurrence ratio of variant samples of the i-th bacterial species.

Images