A traditional Chinese medicine composition for treating jet lag syndrome and its preparation method

Abstract

This invention relates to the field of traditional Chinese medicine, specifically disclosing a traditional Chinese medicine composition for treating jet lag syndrome and its preparation method. The traditional Chinese medicine composition is made from the following raw materials in parts by weight: 20 parts of Acanthopanax senticosus, 12 parts of Polygonatum sibiricum, 12 parts of Salvia miltiorrhiza, 5 parts of Bupleurum chinense, 5 parts of Schisandra chinensis, 3 parts of Scutellaria baicalensis, 3 parts of Glycyrrhiza uralensis (processed), and 9 parts of Ziziphus jujuba. The herbs complement each other, playing a role in tonifying deficiency, promoting blood circulation, and replenishing qi. This can alleviate the symptoms of jet lag syndrome, specifically by shortening the time to adjust to the time difference, improving sleep quality, alleviating depressive mood, and enhancing anti-fatigue ability.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine, and in particular to a traditional Chinese medicine composition for treating jet lag syndrome and its preparation method. Background Technology

[0002] Jet lag syndrome (JLS) is a type of circadian rhythm disorder characterized by a range of psychological and physiological symptoms that occur after flying across multiple time zones. These symptoms primarily include fatigue, insomnia, low mood, indigestion, and headaches. With increasing globalization, at least 2 million people in my country suffer from JLS annually, significantly impacting their work and daily lives. A circadian rhythm is a recurring biological activity with a period of approximately 24 hours, resulting from biological adaptation to periodically changing environments. The biological clock is the intrinsic mechanism that generates and regulates circadian rhythms; at the molecular level, it is composed of a dozen or so clock genes and proteins. When the body's internal biological clock is out of sync with the external environmental clock (such as during cross-time zone flights), both the central and peripheral biological clocks become disordered, leading to a series of symptoms, including JLS.

[0003] Jet lag syndrome falls under the category of "insomnia" in Traditional Chinese Medicine (TCM). The pathogenesis of insomnia is attributed to pathogenic factors overpowering the internal organs, causing defensive qi to circulate in the yang and fail to enter the yin, or to yin deficiency with excessive fire, or to deficiency-heat and irritability due to consumptive diseases. In short, it is mainly caused by an imbalance of yin and yang in the body, generally characterized by an excess of yang and a deficiency of yin, resulting in a disruption of the yin-yang balance. This can be attributed to either yin deficiency preventing the absorption of yang or an excess of yang failing to enter the yin. Therefore, restoring the body's yin-yang balance is an effective way to prevent and treat jet lag syndrome. TCM believes that the human body is an organic whole, with functions changing according to time and exhibiting a diurnal rhythm. Furthermore, TCM emphasizes the correspondence between man and nature, believing that the yin-yang changes within the human body should adapt to the yin-yang changes in nature, driven by the body's biological clock. Therefore, regulating the body's internal biological clock through exogenous substances to adapt the body's rhythm to the external environment is a rapid and effective way to prevent and treat jet lag syndrome. Traditional Chinese medicine has the advantages of being safe, effective, and having few adverse reactions. Gua sha is one of the methods used in the clinical application of jet lag syndrome, but its treatment course is relatively long, and its feasibility for people with fast-paced work schedules needs to be considered. In addition, there are currently no widely available Chinese medicine formulas specifically for jet lag syndrome on the market. Therefore, the development of Chinese medicine decoctions for practical application is of great significance, and developing more Chinese medicine treatment options will be beneficial to the prevention and treatment of this disease.

[0004] Chinese patent application CN201910553010.0 discloses a traditional Chinese medicine granule for regulating sleep and its preparation method, comprising the following raw materials: Rehmannia glutinosa, Acanthopanax senticosus, Schisandra chinensis, Polygonatum sibiricum, Polygonum multiflorum vine, mulberry, and pearl. The formula of this sleep-regulating traditional Chinese medicine granule is derived by combining the pharmacopoeia's recommended raw material dosages, product development requirements, and relevant requirements for health food review, and it has the effect of improving sleep. This traditional Chinese medicine mainly regulates sleep, but it is not suitable for jet lag syndrome, especially for those who cannot regulate their biological clock cycle and recover from jet lag. Summary of the Invention

[0005] To address the aforementioned technical problems, this invention provides a traditional Chinese medicine composition for treating jet lag syndrome and its preparation method.

[0006] To achieve the above objectives, the present invention is implemented according to the following technical solution:

[0007] One objective of this invention is to provide a traditional Chinese medicine composition for treating jet lag syndrome, which is made from the following raw materials in parts by weight:

[0008] Acanthopanax senticosus 20 parts, Polygonatum sibiricum 12 parts, Salvia miltiorrhiza 12 parts, Bupleurum chinense 5 parts, Schisandra chinensis 5 parts, Scutellaria baicalensis 3 parts, Glycyrrhiza uralensis (processed) 3 parts, Ziziphus jujuba 9 parts.

[0009] Preferably, the dosage form of the traditional Chinese medicine composition is a decoction.

[0010] The second objective of this invention is to provide a method for preparing a traditional Chinese medicine composition for treating jet lag syndrome, the specific steps of which are as follows:

[0011] Mix all ingredients thoroughly; add water to cover all ingredients, soak for 30 minutes, bring to a boil over high heat, then simmer over low heat for 40 minutes. Add water again and simmer for another 30 minutes. Collect the decoction to obtain the traditional Chinese medicine composition decoction for treating jet lag syndrome.

[0012] The common pharmacological effects of the traditional Chinese medicine raw materials selected in this invention in this field are as follows:

[0013] Eleutherococcus senticosus: Warm in nature, pungent and bitter in taste. It enters the spleen, kidney, and heart meridians, and has the effects of invigorating qi and strengthening the spleen, tonifying the kidneys and calming the mind. Clinically, it is used for spleen and kidney yang deficiency, soreness and weakness of the lower back and knees, general weakness and fatigue, insomnia, excessive dreaming, and loss of appetite. It is also used to treat injuries from falls and blows.

[0014] Polygonatum: It is neutral in nature and sweet in taste. It enters the lung, spleen and kidney meridians. It has the effects of replenishing qi and nourishing yin, strengthening the spleen, moistening the lungs and benefiting the kidneys. Clinically, it is used to treat spleen and stomach weakness, fatigue, dry mouth and poor appetite, lung deficiency and dry cough, insufficient essence and blood, and internal heat and thirst.

[0015] Salvia miltiorrhiza: It is slightly cold in nature and bitter in taste. It enters the heart and liver meridians. It has the effects of removing blood stasis and relieving pain, promoting blood circulation and regulating menstruation, and clearing the heart and relieving irritability. Clinically, it is used to treat menstrual disorders, amenorrhea and dysmenorrhea, abdominal masses, chest and abdominal pain, hot arthralgia, carbuncles and boils, insomnia, hepatosplenomegaly, and angina pectoris.

[0016] Bupleurum: It is slightly cold in nature and bitter in taste. It enters the liver, gallbladder and lung meridians. It has the effects of harmonizing the exterior and interior, soothing the liver and raising yang. Clinically, it is used to treat colds with fever, alternating chills and fever, chest and rib pain, irregular menstruation, uterine prolapse and rectal prolapse.

[0017] Schisandra chinensis: It is warm in nature, sour and sweet in taste, and enters the lung, heart and kidney meridians. It has the effects of astringing and consolidating, replenishing qi and promoting body fluid, tonifying the kidney and calming the mind. Clinically, it is used to treat chronic cough and wheezing, nocturnal emission and spermatorrhea, enuresis and frequent urination, chronic diarrhea, spontaneous sweating and night sweats, thirst due to fluid depletion, internal heat and thirst, palpitations and insomnia.

[0018] Scutellaria baicalensis: It is cold in nature and bitter in taste. It enters the lung, gallbladder, spleen, large intestine and small intestine meridians. It has the effects of clearing heat and drying dampness, purging fire and detoxifying, stopping bleeding and calming the fetus. Clinically, it is used to treat damp-heat, summer-dampness chest tightness and nausea, damp-heat fullness, diarrhea, jaundice, lung-heat cough, high fever and thirst, blood-heat vomiting and nosebleeds, carbuncles and boils, and threatened abortion.

[0019] Prepared licorice root: It is warm in nature and sweet in taste. It enters the heart, lung, stomach, and spleen meridians. It has the effects of tonifying the spleen and stomach, replenishing qi and restoring pulse. Clinically, it is used to treat spleen and stomach weakness, fatigue, palpitations, and irregular pulse.

[0020] Jujube: It is warm in nature and sweet in taste. It enters the spleen, stomach and heart meridians. It has the effects of replenishing qi and blood and calming the mind. Clinically, it is used to treat spleen deficiency with poor appetite, insufficient qi, blood and body fluids, fatigue and loose stools, and hysteria in women.

[0021] Compared with existing technologies, the traditional Chinese medicine composition of this invention is rigorously formulated and based on the principles of etiology and treatment. Traditional Chinese medicine believes that jet lag syndrome is caused by an imbalance of Yin and Yang in the body, with the main pathogenesis being the invasion of pathogenic factors into the internal organs, causing Wei Qi (defensive Qi) to circulate in the Yang and fail to enter the Yin, or Yin deficiency with exuberant fire and consumptive diseases with fever and irritability. The combination of herbs conforms to the principle of "principal, assistant, adjuvant, and guide" in traditional Chinese medicine, and the formula is scientifically sound. The entire formula contains eight herbs, and the properties and positions of each herb in the formula are as follows: * **Acanthopanax senticosus:** Warm in nature, bitter and pungent in taste, enters the spleen, kidney, and heart meridians, with the functions of strengthening the spleen and replenishing Qi, tonifying the kidneys and calming the mind; it is the principal herb. * **Polygonatum sibiricum:** Neutral in nature, sweet in taste, with the functions of replenishing Qi and nourishing Yin, strengthening the spleen, and tonifying the kidneys; it is also a principal herb. * **Salvia miltiorrhiza:** Slightly cold in nature, bitter in taste, enters the heart and liver meridians, with the functions of removing blood stasis and relieving pain, promoting blood circulation and regulating menstruation, and clearing heat and relieving pain. Irritability, along with other herbs, are the principal herbs; Bupleurum, slightly cold in nature and bitter, enters the liver, lung, and gallbladder meridians, and its functions are to clear the heart and relieve irritability, soothe the liver, and raise yang, serving as the assistant herb; Jujube, warm in nature and sweet in taste, enters the stomach and spleen meridians, and its functions are to tonify the middle energizer, replenish qi, nourish blood, and calm the mind, serving as the assistant herb; Schisandra, warm in nature and sour and sweet in taste, tonifies the kidneys and calms the heart, replenishes qi and generates fluids, serving as the assistant herb; Scutellaria, cold in nature and bitter in taste, clears heat and dries dampness, drains fire and detoxifies, serving as the adjuvant herb; Prepared licorice, warm in nature and sweet in taste, enters the heart, lung, stomach, and spleen meridians, and its functions are to tonify the spleen and stomach, replenish qi and restore pulse, serving as the adjuvant herb; These herbs complement each other, playing a role in tonifying deficiency, activating blood, and replenishing qi, which can alleviate the symptoms of jet lag syndrome, specifically by shortening the time to adjust to the time difference, improving sleep quality, improving depressive mood, and enhancing anti-fatigue ability.

[0022] The traditional Chinese medicine compositions of the present invention all use natural Chinese medicinal materials, the preparation method is simple, the raw materials are widely available, the cost is low, and there are no hormonal stimulant side effects, making them suitable for long-term use. Attached Figure Description

[0023] Figure 1 Figure 1 shows the results of the study on the effects of different concentrations of traditional Chinese medicine compositions on the biorhythmic photometric measurement results and the expression of rhythmic characteristics of the clock gene PER2: (a) Biorhythmic photometric measurement results; (b) Effect on amplitude expression; (c) Effect on phase expression; (d) Effect on period expression.

[0024] Figure 2 The figure shows the results of a study on the effects of different concentrations of traditional Chinese medicine compositions on the activity of mice in a jet lag model.

[0025] Figure 3 The figure shows the results of a study on the effect of different concentrations of traditional Chinese medicine compositions on the recovery time of a time lag model mouse.

[0026] Figure 4 The figure shows the results of the study on the average adaptation time of mice with different concentrations of traditional Chinese medicine compositions in a jet lag model.

[0027] Figure 5 The figure shows the results of a study on the effects of different concentrations of traditional Chinese medicine compositions on the activity cycle of mice in a jet lag model.

[0028] Figure 6The figure shows the results of a study on the effects of different concentrations of traditional Chinese medicine compositions on the activity levels of mice in a jet lag model.

[0029] Figure 7 The figure shows the results of a study on the effects of different concentrations of traditional Chinese medicine compositions on 24-hour sleep in a jet lag model mouse.

[0030] Figure 8 The figure shows the results of a study on the effects of different concentrations of traditional Chinese medicine compositions on sleep during the light exposure period in a jet lag model mouse.

[0031] Figure 9 The figure shows the results of a study on the effects of different concentrations of traditional Chinese medicine compositions on sleep during the dark period in a jet lag model mouse.

[0032] Figure 10 The figure shows the results of a study on the effect of different concentrations of traditional Chinese medicine compositions on immobility time in a forced swimming experiment in a jet lag model mouse.

[0033] Figure 11 The figure shows the results of a study on the effect of different concentrations of traditional Chinese medicine compositions on immobility time in the tail suspension experiment of mice with jet lag.

[0034] Figure 12 Figure 1 shows the results of a study on the anti-fatigue effects of different concentrations of traditional Chinese medicine compositions on a jet lag model mouse. Detailed Implementation

[0035] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to embodiments. The specific embodiments described herein are for illustrative purposes only and are not intended to limit the invention.

[0036] The experimental animals used in the following examples were male C57 / BL6 mice, weighing 18-22g, purchased from Beijing Huafukang Biotechnology Co., Ltd. The mice were housed under alternating day and night lighting conditions and had free access to water.

[0037] Example 1: Preparation of a traditional Chinese medicine composition for treating jet lag syndrome

[0038] Weigh out 20g of Acanthopanax senticosus, 12g of Polygonatum sibiricum, 12g of Salvia miltiorrhiza, 5g of Bupleurum chinense, 5g of Schisandra chinensis, 3g of Scutellaria baicalensis, 3g of Glycyrrhiza uralensis (processed), and 9g of Ziziphus jujuba, and mix them evenly. Add water to cover all the ingredients, soak for 30 minutes, bring to a boil over high heat, then simmer over low heat for 40 minutes. Add water again and simmer for another 30 minutes. Collect the decoction to obtain the traditional Chinese medicine composition decoction for treating jet lag syndrome. Subsequent examples all use this traditional Chinese medicine composition decoction for treating jet lag syndrome.

[0039] Example 2: Regulation of the rhythmic expression of the clock gene PER2 by a traditional Chinese medicine composition

[0040] 1. Experimental Methods

[0041] U2OS cells carrying Per2-Luc (gifted by Researcher Zhang Erquan from the Beijing Institute of Life Sciences) were cultured in six-well plates until the cell density reached 80-90% (control group). The original culture medium was replaced with rhythm monitoring medium containing a traditional Chinese medicine composition (pH 7.2). The rhythm monitoring medium containing the traditional Chinese medicine composition was divided into a low-dose group and a high-dose group. The low-dose group consisted of low-glucose DMEM (containing 0.29 mg / mL L-glutamine), 3.5 mg / mL L-glucose, 0.35 mg / mL sodium bicarbonate, 10 mM HEPES, 10% FBS, 100 U / mL penicillin, 100 μg / mL streptomycin, 0.1 mM Beetle Luciferin (luciferase substrate), and SCHJ-50 μg / mL. The high-dose group consisted of low-glucose DMEM (containing 0.29 mg / mL L-glutamine), 3.5 mg / mL... Composed of D-glucose, 0.35 mg / mL sodium bicarbonate, 10 mM HEPES, 10% FBS, 100 U / mL penicillin, 100 μg / mL streptomycin, 0.1 mM Beetle Luciferin (luciferase substrate), and SCHJ-100 μg / mL.

[0042] 2. Cell treatment and indicator observation

[0043] After sealing the petri dish with sealing film, place it in a LumiCycle 32-channel biorhythmic photometer for real-time monitoring for 5 days (results see [link]). Figure 1 In section (a), the rhythmic characteristics (period, phase, and amplitude) of Per2-Luc cells were analyzed using Lumicycle software. Rhythmic oscillation signals of Per2-Luc expression in U2OS cells were continuously recorded. After the experiment, the raw data were exported, and the period, phase, and amplitude were calculated using LumicycleAnalysis software.

[0044] 3. Results

[0045] The above experimental results show that, compared with the control group, the amplitude of the PER2 gene was significantly increased in the SCHJ-50μg / ml group and the SCHJ-100μg / ml group, with the increase in amplitude being more pronounced in the SCHJ-100μg / ml group (see [link to experimental results]). Figure 1 (b)). Meanwhile, compared to the control group, the phase of the PER2 gene was shifted backward in the SCHJ-50 μg / ml group, while no significant phase change was observed in the SCHJ-100 μg / ml group (see [reference]). Figure 1(c)). Furthermore, it was found that the PER2 gene cycle was slightly prolonged in the SCHJ-50 μg / ml group compared to the control group, while no significant change was observed in the SCHJ-100 μg / ml group (see [reference]). Figure 1 (d) in the middle.

[0046] Example 3: Traditional Chinese medicine composition shortens recovery time after jet lag

[0047] 1. Grouping and administration

[0048] C57BL / 6 mice were placed in a 12-hour light / 12-hour dark environment for 5 days and were divided into three groups. The mice were intraperitoneally injected with blank solvent (physiological saline, 10 μL / g), SCHJ-50 mg / kg (low-dose group of the traditional Chinese medicine composition), and SCHJ-100 mg / kg (high-dose group of the traditional Chinese medicine composition), respectively, once daily for 5 consecutive days. Simultaneously, the daily light exposure time was advanced by 8 hours, and spontaneous activity of the mice was monitored and analyzed using an animal running wheel Clocklab data acquisition and analysis system.

[0049] 2. Results

[0050] An acute jet lag model was constructed using the classic method of 8-hour advance lighting. After jet lag, mice in the solvent group took an average of 6 days to adapt to the 8-hour advance; mice in the low-dose group (50 mg / kg) of the traditional Chinese medicine composition took an average of 4 days to adapt; and mice in the high-dose group (100 mg / kg) of the traditional Chinese medicine composition took an average of only 2 days to adapt (see [link to relevant documentation]). Figure 2 and Figure 3 Compared with the solvent control group, the number of days with the 50% phase shift was reduced in the low-dose group of the traditional Chinese medicine composition, and the difference was statistically significant; the reduction in the number of days with the 50% phase shift was even more significant in the high-dose group of the traditional Chinese medicine composition, and the result was statistically significant (see [link to relevant documentation]). Figure 4 Furthermore, compared to the solvent group, the herbal composition (low-dose and high-dose groups) had no effect on the activity cycle of mice (see [link]). Figure 5 Analysis of the average daily activity levels of the three groups of mice revealed that the traditional Chinese medicine composition (low-dose and high-dose groups) significantly increased the activity levels of the mice, primarily by increasing their activity levels during the night (see [link to relevant documentation]). Figure 6 ).

[0051] Example 4: Effects of Traditional Chinese Medicine Composition on Sleep in Jet Lag Mice

[0052] 1. Implantation of electroencephalogram (EEG) recording electrodes

[0053] Mice were anesthetized and fixed on a sterile stereotaxic apparatus. The area was disinfected with povidone-iodine, the scalp was cut open, the meninges were removed, and the anterior fontanelle was located. The frontal lobe region (AP 1.80, ML±0.40) was determined based on the mouse brain atlas. A hole was made using a cranial drill, and recording electrodes were implanted in the frontal lobe, with a reference electrode placed in the cerebellum. Two gold wires were inserted bilaterally into the neck muscles to record electromyography (EMG). After the electrodes were installed, they were fixed with dental cement. Related experiments were performed one week after surgery. EEG and EMG data were acquired using the Medusa EEG and EMG data acquisition system and analyzed using LabChart 8.0 software.

[0054] 2. Grouping and Processing

[0055] Mice with implanted electrodes were divided into three groups and injected intraperitoneally with blank solvent (physiological saline, 10 μL / g), SCHJ-50 mg / kg, and SCHJ-100 mg / kg, respectively, once daily for 5 consecutive days. Starting from day 6, the start time of light exposure was advanced by 8 hours each day. Electroencephalography (EEG) and electromyography (EMG) were recorded simultaneously, and the mice's sleep was analyzed.

[0056] 3. Electroencephalogram (EEG) signal analysis

[0057] After 7 consecutive days of EEG / EMG recording, the EEG / EMG data were analyzed using LabChart 8.0 software. The sampling rate of EEG and EMG signals was 500Hz, and the signals were amplified and filtered (EMG filtering range was 10-500Hz, and EEG high-pass filtering was 1.0Hz). The EEG and EMG signals were analyzed in 10s units, and wakefulness, NREM sleep, and REM sleep were determined according to the following criteria: (1) wakefulness (high amplitude EMG activity and low amplitude EEG signal); (2) NREM sleep (high amplitude EEG activity with δ wave dominance and low amplitude EMG signal); (3) REM sleep (low amplitude EMG activity with θ wave dominance and no EMG signal). After analysis, the wakefulness, NREM sleep, and REM sleep times of different groups of mice during the light and dark periods and 24 hours were recorded.

[0058] 4. Results

[0059] Using EEG and EMG data, we were able to examine the effects of the traditional Chinese medicine composition on sleep in jet lag mice. Compared with the solvent group, mice in the 50 mg / kg and 100 mg / kg dose groups showed shorter wakefulness time and longer NREM and REM sleep durations throughout the day in a dose-dependent manner, indicating that the traditional Chinese medicine composition can significantly improve sleep in jet lag mice (see [link to relevant documentation]). Figure 7Based on this, we further analyzed the effects of the traditional Chinese medicine composition on various sleep stages in mice during the light and dark periods. Compared with the solvent group, mice in the 50 mg / kg and 100 mg / kg dose groups showed shorter wakefulness time and longer NREM and REM sleep durations during the light period, with the most significant effect on REM sleep duration (see [link to study].) Figure 8 In addition, the herbal composition significantly increased NREM and REM sleep duration during the dark period, but had no effect on wakefulness (see [link]). Figure 9 The above results indicate that this traditional Chinese medicine composition can improve sleep in mice experiencing jet lag.

[0060] Example 5: Effects of a Traditional Chinese Medicine Composition on Depressive-like Behavior in Jet Lag Mice

[0061] 1. Grouping and processing

[0062] C57BL / 6 mice were placed in a 12-hour light / 12-hour dark environment for 5 days and were divided into three groups. The mice were intraperitoneally injected with blank solvent (physiological saline, 10 μL / g), SCHJ-50 mg / kg, and SCHJ-100 mg / kg, respectively, once a day for 5 consecutive days. Starting from day 6, the light exposure time was advanced by 8 hours.

[0063] 2. Depressive-like behavior detection

[0064] The effects of traditional Chinese medicine compositions on depressive-like behavior in mice were mainly evaluated using the forced swimming test (FST) and the tail suspension test (TST). The forced swimming test was performed as follows: all mice underwent pre-swimming the day before the experiment. After adjusting to the time difference, the mice were placed in a glass cylinder (20 cm high, 14 cm in diameter) with a water depth of 10 cm and a water temperature of 22–24 °C. They swam for 6 minutes, and the immobility time within the last 4 minutes was recorded. The swimming test primarily measured immobility time, i.e., the time the mouse stopped struggling in the water and floated with only its head above the surface. The tail suspension test was performed as follows: after adjusting to the time difference, the tails of mice in different groups were fixed 1 cm from the end with tape, suspending the mice upside down with their heads 15 cm above the platform. This position was maintained for 6 minutes, and the immobility time within the last 4 minutes was recorded.

[0065] 3. Results

[0066] After jet lag, mice showed a significant increase in immobility time in both the forced swimming test (FST) and the tail suspension test (TST), indicating that jet lag increases depressive-like behavior in mice. Figure 10 and Figure 11 Compared with the solvent group, mice in the 50 mg / kg and 100 mg / kg dose groups showed significantly shorter immobility time in the forced swimming test (FST), and this reduction was dose-dependent (see [link to relevant documentation]). Figure 10Mice in the 50 mg / kg and 100 mg / kg dose groups also showed significantly less immobility time in the tail suspension test (TST), with higher doses showing a more significant improvement (see [link to original text]). Figure 11 This indicates that the traditional Chinese medicine composition can significantly improve depressive-like behavior in mice after jet lag.

[0067] Example 6: Effect of Traditional Chinese Medicine Composition on Anti-fatigue Ability of Mice Reversing Jet Lag

[0068] 1. Grouping and processing

[0069] C57BL / 6 mice were placed in a 12-hour light / 12-hour dark environment and divided into three groups. The mice were intraperitoneally injected with blank solvent (physiological saline, 10 μL / g), SCHJ-50 mg / kg, and SCHJ-100 mg / kg, respectively, once daily for 5 consecutive days. Starting from day 6, the light exposure time was advanced by 8 hours.

[0070] 2. Material processing and indicator observation

[0071] On the day of the test, mice should be kept in their own cages and allowed to acclimatize in the testing room for at least 15 minutes (acclimatization phase). Weigh each mouse and record its weight on the data sheet, then turn on the rotarod device. Place the mice in the aisles and try to get them to walk forward on the bar to maintain their balance. The bar initially rotates at a constant speed of 4 rpm to allow all mice to stay in their respective aisles. After startup, the bar will accelerate from 4 rpm to 40 rpm within 300 seconds. Throughout the experiment, the rotarod device will automatically record the latency, fall speed, and distance traveled by each mouse when it falls from the bar, and record the reason for the end of the experiment (fall, jump, passive rotation).

[0072] 3. Results

[0073] The rotarod fatigue test is mainly used to assess the motor function of mice. Compared with the solvent group, the drop latency of mice in the 50 mg / kg and 100 mg / kg dose groups was significantly prolonged in a dose-dependent manner, and the results were statistically significant. This indicates that the traditional Chinese medicine composition has the effect of enhancing the motor function of mice, and the higher the dose, the better the anti-fatigue effect (see...). Figure 12 ).

[0074] In summary, the traditional Chinese medicine composition described in this invention and its application in mice with jet lag syndrome demonstrate that the compound significantly improves jet lag syndrome, specifically by shortening the adaptation time, improving sleep quality, alleviating depressive mood, and enhancing anti-fatigue ability. Furthermore, because the gene sequences of mice and humans are highly similar, even their chromosomes are very close (humans have 42 pairs of chromosomes, while mice have 40 pairs), mice are chosen as experimental subjects because similar symptoms can be found in mice to identify treatment methods. Moreover, the traditional Chinese medicine composition used in this invention employs natural medicinal materials and has no hormonal or toxic side effects, thus making it suitable for treating human jet lag syndrome.

[0075] The technical solutions of the present invention are not limited to the specific embodiments described above. Any technical modifications made in accordance with the technical solutions of the present invention fall within the protection scope of the present invention.

Claims

1. A traditional Chinese medicine composition for treating jet lag, characterized in that, The traditional Chinese medicine composition is made from the following raw materials by weight: Radix acanthopanacis 20 parts, Rhizoma polygonati 12 parts, Radix salviae miltiorrhizae 12 parts, Radix bupleuri 5 parts, Fructus schisandrae 5 parts, Radix scrophulariae 3 parts, Radix glycyrrhizae preparata 3 parts, Fructus jujubae 9 parts.

2. The traditional Chinese medicine composition for treating jet lag according to claim 1, characterized in that: The dosage form of the traditional Chinese medicine composition is a decoction.

3. The method of claim 2, wherein the herbal composition is prepared by mixing the following ingredients: The specific steps are as follows: ​ Mix all the raw materials evenly; add water to cover all the raw materials, soak for 30 min, boil with high heat, and then continue to cook with small heat for 40 min; add water again for the second time and continue to cook for 30 min; collect the decoction to obtain the traditional Chinese medicine composition decoction for treating jet lag syndrome.

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