Brain donation in situ freezing box

Abstract

This invention discloses a donated brain in situ cryopreservation box in the field of biological research technology, comprising: a box body; an inner wall of the box body having an installation layer, on which a first partition is provided, dividing the installation layer into first sections; a second partition is provided on the installation layer within each first section, dividing the installation layer within the first section into second sections; slots are provided on the installation layers within each second section, each slot having a cryopreservation tube and a locking component for locking the cryopreservation tube; a drive component for individually adjusting the locking component is provided within the installation layer; a partition cover is provided on the top of each first partition; a pressing component is also provided on the top of the first partition, used to control the opening and closing of the partition cover and the operation of the drive component. This solution has the advantages of rapid fixation, rapid retrieval and placement of cryopreservation tubes, and easy and quick location of the required sample.

Description

Technical Field

[0001] This invention belongs to the field of biological research technology, specifically the donation of brain cryopreservation kits. Background Technology

[0002] The National Brain Tissue Resource Bank for Health and Disease (hereinafter referred to as the Brain Bank) was established to meet the needs of scientific research by integrating experts and resources from neuroscience, human anatomy, pathology, and other related disciplines. The Brain Bank collects and stores post-mortem brain tissue donated by patients with various neuropsychiatric diseases and healthy controls, along with their ante-death medical histories, according to international standards. It also conducts detailed and accurate neuropathological diagnoses (i.e., "final diagnoses") on these brain tissue samples. Through a formally approved application and approval process for human brain tissue research, the Brain Bank provides relevant brain tissue samples, (anonymous) ante-death medical histories, and neuropathological diagnostic reports to scientists for research. The aim is to discover and elucidate the causes of human neuropsychiatric diseases, such as Alzheimer's disease, Parkinson's disease, depression, and schizophrenia, providing scientists with the most direct and effective research materials to find related pathogenesis mechanisms and establish effective treatment methods.

[0003] Current techniques primarily involve preserving the cerebral cortex by cutting it into 157 pieces according to the Brodmann partition (a system that divides the cerebral cortex into a series of anatomical regions based on cellular structure). These pieces are then stored at -80°C, typically in liquid nitrogen tanks. However, the large number of pieces and the potential for confusion between samples from different donors and different brain regions make subsequent retrieval difficult and extraction challenging.

[0004] Therefore, there is an urgent need for a donated brain cryopreservation kit that can classify and preserve the segmented samples according to the brain structure, so as to quickly determine the brain region where the sample is located during later retrieval, thereby facilitating rapid sample retrieval and placement. Summary of the Invention

[0005] To address the aforementioned problems, the purpose of this invention is to provide an in situ cryopreservation box for donated brains, which can classify and preserve samples according to their distribution location on the brain, shortening the time required for subsequent sample retrieval and thus improving the convenience of sample retrieval and placement.

[0006] To achieve the above objectives, the technical solution of the present invention is as follows:

[0007] Donate brain cryopreservation kits, including: the kit body;

[0008] The inner wall of the box is provided with an installation layer, and a first enclosure plate is provided on the installation layer, which divides the installation layer into first sections; a second enclosure plate is provided on the installation layer in each first section, which divides the installation layer in the first section into second sections; a slot is provided on the installation layer in each second section, and a cryopreservation tube and a locking component for locking the cryopreservation tube are provided on the slot; a drive component for adjusting the locking component is provided in the installation layer.

[0009] Each of the first enclosure panels has a partition cover on its top; a pressing component is also provided inside the top of the first enclosure panel, which is used to control the opening and closing of the partition cover and to control the operation of the drive component.

[0010] The above approach has the following beneficial effects:

[0011] 1. This solution divides the box into two sections: a first section and a second section. Slots are set according to the second section, which makes it easier to find the corresponding position when placing the cryopreservation box and achieve classified preservation.

[0012] 2. This solution incorporates a locking mechanism to ensure greater stability when the cryopreservation tubes are placed on the box, preventing the box from shaking and causing the cryopreservation tubes to deviate from their original positions, thus avoiding confusion.

[0013] 3. This solution controls the opening and closing of the partition cover by pressing the component, and simultaneously controls the locking component. This allows staff to release the cryovials as the partition cover is opened, enabling faster retrieval and placement of cryovials. Compared to traditional methods, such as opening the cover, adjusting the cryovial's tightness, and then retrieving it, or placing the cryovial, clamping it, and then closing the cover, this solution combines the processes of opening the cover and adjusting the cryovial's tightness into one step, thus shortening the retrieval and placement time and increasing efficiency.

[0014] In summary, this method has the advantages of rapid fixation and quick retrieval of cryovials, as well as the ability to quickly locate the required sample.

[0015] Furthermore, the locking assembly includes a carrier and a locking pin disposed at the bottom of the cryopreservation tube; the top of the carrier is provided with an insertion hole, and the locking pin slides into the insertion hole; a second movable groove is provided inside the carrier; the second movable groove communicates with the insertion hole; an inverted metal locking cup is provided above the second movable groove, and the inner diameter of the bottom of the cup is smaller than that of the cup body, and the locking cup slides into the second movable groove; a first spring is provided inside the locking cup; the bottom of the locking cup is provided with a through groove and several radial grooves arranged circumferentially with the through groove, the through groove communicating with the radial grooves respectively, and a locking ball is provided in the radial groove, the locking ball slidingly engaging with the radial groove and the second movable groove respectively, wherein the side of the locking ball near the through groove intersects with the extension line of the insertion hole in the length direction.

[0016] Beneficial effects: When the locking pin is inserted into the socket, as it enters the through groove, the locking pin pushes the locking ball away from both sides along the radial groove. The locking ball slides downward along the side wall of the second movable groove. At the same time, the first spring is subjected to downward pressure from the locking cup. After the locking pin is fully inserted, the first spring pushes the locking cup upward, and the locking ball slides upward along the side wall of the second movable groove. During the upward sliding of the locking ball, the side wall of the second movable groove squeezes the locking ball towards the center of the through groove, causing the locking ball to gradually lock the locking pin, thereby completing the fixation of the cryopreservation tube.

[0017] Furthermore, the drive component includes a first electromagnet disposed below the mounting layer within each of the second partitions.

[0018] Beneficial effects: When the cryopreservation tube needs to be removed, the first electromagnet is activated to generate magnetic force, which attracts the locking cup. After being attracted, the locking cup moves downward along the second movable groove. During the downward movement of the locking cup, the locking ball slides to both sides along the radial groove, releasing the locking pin from its locked state, so that the locking pin can be smoothly removed from the socket.

[0019] Furthermore, the pressing component includes a first movable groove disposed within the top of the first enclosure; a movable block disposed within the first movable groove, the movable block slidingly engaging with the first movable groove; a second spring fixedly connected between the bottom of the movable block and the bottom of the first movable groove; a connecting rod disposed on the side wall of the movable block, one end of the connecting rod rotatably connected to the movable block, and the other end of the connecting rod having a cylindrical protrusion; a touch switch disposed within the top of the movable block, the touch switch being electrically connected to an electromagnet; a limit protrusion and a stop block disposed on the side wall of the first movable groove, the limit protrusion slidingly engaging with the cylindrical protrusion, and the stop block being used to push the cylindrical protrusion back above the limit protrusion; wherein, the end of the partition cover furthest from the first movable groove is rotatably connected to the first enclosure, the bottom of the end of the partition cover closest to the first movable groove has a first engaging protrusion, and the top of the movable block also has a second engaging protrusion, the first engaging protrusion engaging with the second engaging protrusion.

[0020] Beneficial effects: When the cryopreservation tube needs to be retrieved, pressing the partition cover of the target area of ​​the desired cryopreservation tube causes the end of the partition cover near the first movable groove to be squeezed downwards. The first engaging protrusion squeezes the movable block, causing the movable block to move downwards. The cylindrical protrusion slides along the surface of the limiting protrusion until it slides from the bottom of the limiting protrusion to the top of the limiting protrusion. Then, under the action of the second spring, the movable block bounces upwards, and the engagement between the first engaging protrusion and the second protrusion is disengaged. At this time, the partition cover opens. Simultaneously, as the partition cover opens, the first engaging protrusion squeezes the top of the movable block downwards, triggering the touch switch located at the top of the movable block. This drives the first electromagnet, causing it to attract the locking cup and release the locking ball from its locked state, allowing the operator to retrieve the cryopreservation box after opening the partition cover. When the partition cover is closed, the first engaging protrusion engages with the second engaging protrusion, and the touch switch is triggered again, causing the first electromagnet to disconnect. After the magnetic force disappears, the locking ball returns to its locked state, completing the self-fixation.

[0021] Furthermore, it also includes a second electromagnet, a display, and a controller located below the first movable slot; the display is used to receive information; the controller is used to determine which partition cover needs to be opened based on the received information; the controller is also electrically connected to the first electromagnet and the second electromagnet; wherein, the movable block is made of metal.

[0022] Beneficial effects: When personnel unfamiliar with brain regions (such as trainees) retrieve cryopreservation tubes, they can input the required cerebral cortex sample information through the display. The controller will match the corresponding partition cover based on the input information and open the partition cover, so that trainees can quickly and accurately retrieve and place cryopreservation tubes.

[0023] Furthermore, a first spring groove is provided at the bottom of the second movable groove, and the side wall of the first spring groove corresponds to the side wall of the locking cup.

[0024] Beneficial effects: By setting a first spring groove at the bottom of the second movable groove, the first spring can be prevented from deflecting during deformation. At the same time, the side wall of the first spring groove can limit the descent height of the locking cup, preventing the locking pin from falling too far and damaging the bottom of the second movable groove.

[0025] Furthermore, the first region is divided into the frontal lobe, parietal lobe, occipital lobe, and temporal lobe regions.

[0026] Beneficial effects: By initially dividing the area into frontal lobe, parietal lobe, occipital lobe and temporal lobe, it is easier to quickly locate the required cryopreservation tube (the cryopreservation tube contains a section of the cerebral cortex).

[0027] Furthermore, the second partition is partitioned according to the Broadman partitioning system.

[0028] Beneficial effects: Broadman partitioning allows for more precise placement of cryopreservation tubes (i.e., cut cerebral cortex).

[0029] Furthermore, the box is shaped like a brain.

[0030] Beneficial effects: By shaping the box into the shape of the human brain, it helps medical personnel quickly determine the location of the required cerebral cortex based on experience.

[0031] Furthermore, the slots are arranged along the gyri of the brain.

[0032] Beneficial effects: By arranging the slots along the gyri, it is easier to store the tissue according to the original site of the incision, so as to quickly and accurately locate the cryopreservation tubes for placing the cerebral cortex. Attached Figure Description

[0033] Figure 1 This is a schematic diagram of the structure of Embodiment 1 of the present invention.

[0034] Figure 2 for Figure 1 A schematic diagram of the box after the partition cover is removed.

[0035] Figure 3 for Figure 2 A cross-sectional schematic diagram of one of the second sections.

[0036] Figure 4 for Figure 3 A schematic diagram of the locking assembly.

[0037] Figure 5 for Figure 3 A schematic diagram of the structure of the middle pressing component.

[0038] Figure 6 This is a schematic diagram of the cryopreservation tube in Embodiment 1 of the present invention.

[0039] Figure 7 This is a block diagram of the control system in Embodiment 2 of the present invention. Detailed Implementation

[0040] Embodiments of the present invention are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention.

[0041] In the description of this invention, it should be understood that the terms "longitudinal", "lateral", "vertical", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", and "outer" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0042] In the description of this invention, unless otherwise specified and limited, it should be noted that the terms "installation", "connection" and "linking" should be interpreted broadly. For example, they can refer to mechanical or electrical connections, or internal connections between two components. They can be direct connections or indirect connections through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms according to the specific circumstances.

[0043] The following detailed description illustrates the specific implementation method:

[0044] The reference numerals in the accompanying drawings include: box body 1, slot 2, cryopreservation tube 3, first electromagnet 4, touch switch 5, carrier 6, second electromagnet 7, display 8, partition cover 101, first engaging protrusion 1011, first enclosure 102, first movable groove 1021, limiting protrusion 1022, stop block 1023, second enclosure 1024, movable block 103, connecting rod 1031, cylindrical protrusion 1032, second engaging protrusion 1033, insertion hole 201, second movable groove 202, locking cup 203, locking ball 204, radial groove 2041, through groove 2042, first spring 205, first spring groove 206, and locking pin 301.

[0045] Example 1

[0046] The basics are as follows: Figures 1-6 As shown:

[0047] Donate a brain cryopreservation box, which includes box 1, which is shaped like a brain, specifically the left hemisphere.

[0048] The inner wall of the box 1 is bonded with an installation layer, and a first enclosure 102 is welded onto the installation layer. The first enclosure 102 divides the installation layer into frontal lobe, parietal lobe, occipital lobe and temporal lobe regions. Each region is marked by color, shape or text. A second enclosure 1024 is welded onto the installation layer of each frontal lobe, parietal lobe, occipital lobe and temporal lobe region. The second enclosure 1024 divides the installation layer in the first region according to the Broadman partitioning system. Each installation layer partitioned according to the Broadman partitioning system has a slot 2. The slot 2 is arranged along the gyri of the brain. Each slot corresponds to the region of the cerebral cortex after partitioning by the Broadman partitioning system. For samples located below the surface of the cerebral cortex, such as the insula and basal ganglia and midbrain region, the slot 2 is located at the mapping position of the insula and basal ganglia and midbrain region on the surface of the cerebral cortex. Each slot 2 is equipped with a cryopreservation tube 3 and a locking component for locking the cryopreservation tube 3.

[0049] The locking assembly includes a carrier 6 ( Figure 4 ) and the locking pin 301 located at the bottom of the cryopreservation tube 3 Figure 6 The carrier 6 has an insertion hole 201 at its top, and the locking pin 301 slides into the insertion hole 201; a second movable groove 202 is provided inside the carrier 6; the second movable groove 202 communicates with the insertion hole 201; as shown in the attached figure. Figure 4 As shown, an inverted metal locking cup 203 is provided above the second movable groove 202, and the inner diameter of the bottom of the cup is smaller than that of the cup body. The locking cup 203 slides in conjunction with the second movable groove 202. A first spring groove 206 is also welded to the bottom of the second movable groove 202. The side wall of the first spring groove 206 corresponds to the side wall of the locking cup 203, and the side wall of the first spring groove 206 and the side wall of the locking cup 203 can overlap. The locking cup 203 contains a first spring 205, and the two ends of the first spring 205 are welded and fixed to the first spring groove 206 and the locking cup 203, respectively. The bottom of the locking cup 203 is provided with a through groove 2042 and a number of radial grooves 2041 arranged circumferentially around the through groove 2042 (specifically, three radial grooves 2041 in this embodiment, equidistantly distributed around the through groove 2042). The radial grooves 2041 are all connected to the through groove 2042. A locking ball 204 is placed in the radial groove 2041. The locking ball 204 slides with the radial groove 2041 and the second movable groove 202, respectively. The side of the locking ball 204 closer to the through groove 2042 intersects the extension line of the insertion hole 201 in the length direction, and the side of the locking ball 204 away from the through groove 2042 slides with the second movable groove 202.

[0050] The mounting layer is equipped with drive components for individually adjusting the locking components. In this embodiment, the drive components specifically include a first electromagnet 4 bolted to the bottom of the mounting layer in each first partition. In this solution, both the first electromagnet 4 and the second electromagnet 7 are low-temperature resistant electromagnets. The magnetic force generated by each first electromagnet 4 can attract all the locking cups 203 in its respective second partition, thereby adjusting the locking components.

[0051] Each of the first enclosure panels 102 has a partition cover 101 on its top. A pressing component is also provided inside the top of the first enclosure panel 102, used to control the opening and closing of the partition cover 101 and the operation of the drive component. In this embodiment, the pressing component includes a first movable groove 1021 formed inside the top of the first enclosure panel 102; a movable block 103 is provided inside the first movable groove 1021, and the movable block 103 slides in conjunction with the first movable groove 1021; a second spring is welded and fixed between the bottom of the movable block 103 and the bottom of the first movable groove 1021; a connecting rod 1031 is provided on the side wall of the movable block 103, one end of the connecting rod 1031 is hinged to the movable block 103, and a cylindrical protrusion 1032 is welded to the other end of the connecting rod 103; a touch switch 5 is provided inside the top of the movable block 103 (specifically, a space for accommodating the touch switch 5 is formed inside the top of the movable block 103). The groove is used to bolt the touch switch 5 (model TS-1187C) and is electrically connected to the electromagnet. A limit protrusion 1022 and a stop block 1023 are welded to the side wall of the first movable groove 1021 near the cylindrical protrusion 1032. The limit protrusion 1022 slides with the cylindrical protrusion 1032, and the stop block 1023 is used to push the cylindrical protrusion 1032 back above the limit protrusion 1022 (specifically, during the upward reset process of the inclined connecting rod 1031, the rod body of the connecting rod 1031 contacts the stop block 1023, and the connecting rod 1031 transitions from the inclined direction to the vertical direction under the obstruction of the stop block 1023). The end of the partition cover 101 away from the first movable groove 1021 is hinged to the top of the first enclosure plate 102. A first engaging protrusion 1011 is welded to the bottom of the end of the partition cover 101 near the first movable groove 1021. A second engaging protrusion 1033 is also welded to one side of the top of the movable block 103. The first engaging protrusion 1011 and the second engaging protrusion 1033 are engaged and connected.

[0052] The specific implementation process is as follows:

[0053] When placing or removing cryovial 3, medical personnel will place the left cerebral cortex, cut according to the Brodman partitioning system, into cryovial 3, and then press the partition cover 101 corresponding to the cut location. For example, when placing the frontal lobe cortex, as shown... Figure 5As shown, by pressing the partition cover 101 above the frontal lobe, the end of the partition cover 101 above the frontal lobe near the first movable groove 1021 is deflected downwards. The first engaging protrusion 1011 at the bottom of the partition cover 101 above the frontal lobe moves downwards, pressing the movable block 103 downwards. During the downward movement of the movable block 103, the cylindrical protrusion 1032 at one end of the connecting rod 1031 moves counterclockwise from the bottom of the limiting protrusion 1022 along the surface of the limiting protrusion 1022. During the movement, the connecting rod 1031 is restricted in its direction of movement and rotation by the stop block 1023 until the cylindrical protrusion 1032 moves above the limiting protrusion 1022 (as shown in the attached figure). Figure 5 As shown), upon release, the movable block 103 is lifted by the second spring, and the second engaging protrusion 1033 deflects outward, disconnecting the first engaging protrusion 1011 and the second engaging protrusion 1033, thereby opening the partition cover 101 above the frontal lobe. Simultaneously, when the first engaging protrusion 1011 contacts the top of the sliding block, it triggers the touch switch 5. The touch switch 5 is electrically connected to the electromagnet, so when the touch switch 5 is triggered, it controls the first electromagnet 4 below the corresponding frontal lobe area to operate. The first electromagnet 4 generates magnetic force that attracts the locking cup 203 downward.

[0054] Then, based on the experience of medical staff, the corresponding slot 2 (i.e., the position cut according to the Broadman partition) is quickly located from the frontal lobe area of ​​box 1, and then the cryopreservation tube 3 is inserted into slot 2.

[0055] (Operating process of the locking assembly when the first electromagnet 4 does not generate magnetic force) During the insertion of the cryopreservation tube 3, the locking pin 301 enters the through groove 2042 along the insertion hole 201 and comes into frictional contact with the locking ball 204. As the locking pin 301 moves downward, it pushes the locking ball 204 outward along the radial groove 2041. At the same time, the locking ball 204 moves downward along the inner wall of the second movable groove 202 (as shown in the attached figure). Figure 4 As shown in the diagram, after the medical staff releases their grip, the first spring 205 pushes the locking cup 203 upward, and the locking ball 204 slides inward along the radial groove 2041 as it slides upward along the second movable groove 202, thereby locking the locking pin 301 and completing the fixation of the cryopreservation tube 3. When the first electromagnet 4 generates magnetic force, the locking cup 203 is attracted downward. At this time, a sufficient gap is generated between the locking ball 204 and the second movable groove 202, allowing the locking ball 204 to slide freely in the radial groove 2041. That is, the locking ball 204 will be released from its locked state, so that the cryopreservation tube 3 can be inserted and removed from the insertion hole 201.

[0056] Then press the partition cover 101 again. The first engaging protrusion 1011 moves downward, pressing the movable block 103 downward again. At this time, the cylindrical protrusion 1032 rotates counterclockwise from the top of the limiting protrusion 1022 along the surface of the limiting protrusion 1022 until the cylindrical protrusion 1032 moves to the bottom of the limiting protrusion 1022, thereby fixing the movable block 103. At this time, the first engaging protrusion 1011 and the second engaging protrusion 1033 engage and connect. At the same time, the first engaging protrusion 1011 will touch the trigger switch again. 5. The touch switch 5 de-energizes the first electromagnet 4. After the locking cup 203 loses its magnetic attraction, it is pushed upward by the first spring 205, causing the locking ball 204 to lock the locking pin 301 again (the specific principle is that the locking ball 204 locks the locking pin 301, and the friction between the locking pin 301 and the locking ball 204 is related to the position of the locking ball 204. The more force is applied to pull the locking pin 301 upward, the greater the squeezing force of the locking ball 204 on the locking pin 301). This completes the storage and fixation of the cryopreservation tube 3.

[0057] The entire process can be summarized as follows: Pressing the partition cover 101 opens the partition cover 101, energizing the first electromagnet below the partition cover 101, which releases the frozen tube 3 below the partition cover 101 from its locked state, allowing it to be freely removed and placed. After the removal and placement are completed, the partition cover 101 is closed, the first electromagnet 4 is de-energized, and the frozen tube 3 is locked again.

[0058] Example 2

[0059] The only difference from the above embodiment is that it also includes a second electromagnet 7, a display 8, and a controller disposed below the first movable slot 1021; the display 8 is used to receive information and is electrically connected to the controller; the controller is used to determine the partition cover 101 that needs to be opened based on the received information; the controller is also electrically connected to the first electromagnet and the second electromagnet; wherein, the movable block 103 is made of metal material.

[0060] In specific implementation: see attached Figure 7 As shown, when a trainee medical staff member (someone who is not familiar with brain regions) retrieves or places cryopreservation tube 3, the anatomical region to which the cut cerebral cortex belongs (e.g., inferior frontal gyrus) is input via the display 8. Based on the input information (inferior frontal gyrus), the controller determines that the inferior frontal gyrus belongs to the frontal lobe region. Then, it controls the second electromagnet 7 to work intermittently. The second electromagnet 7 generates magnetic force to attract the movable block 103 downward (simulating the action of the staff member pressing the partition cover 101), thereby opening the partition cover 101 of the frontal lobe region. At the same time, it controls the first electromagnet to work, and the locking ball 204 below the frontal lobe region is released from its locked state (without affecting the locking balls 204 of other regions, i.e., the cryopreservation tubes 3 in other regions are all locked by the locking balls 204), so that the trainee medical staff can retrieve or place cryopreservation tubes 3.

[0061] The above descriptions are merely embodiments of the present invention, and common knowledge such as specific structures and / or characteristics in the solutions are not described in detail here. It should be noted that those skilled in the art can make various modifications and improvements without departing from the structure of the present invention, and these should also be considered within the scope of protection of the present invention. These modifications and improvements will not affect the effectiveness of the implementation of the present invention or the practicality of the patent. The scope of protection claimed in this application should be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.

Claims

1. A brain cryopreservation kit for donation, characterized by: Box body; The box is shaped like a brain; The inner wall of the box is provided with an installation layer, and a first enclosure plate is provided on the installation layer, which divides the installation layer into first sections; a second enclosure plate is provided on the installation layer in each first section, which divides the installation layer in the first section into second sections; a slot is provided on the installation layer in each second section, and a cryopreservation tube and a locking component for locking the cryopreservation tube are provided on the slot; a drive component for adjusting the locking component is provided in the installation layer. Each of the first enclosure panels is equipped with a partition cover at the top; a pressing component is also provided inside the top of the first enclosure panel, which is used to control the opening and closing of the partition cover and to control the operation of the drive component. The first region is divided into the frontal lobe, parietal lobe, occipital lobe, and temporal lobe. The second partition is partitioned according to the Broadman partitioning system.

2. The donated brain in situ cryopreservation kit according to claim 1, characterized in that: The locking assembly includes a carrier and a locking pin located at the bottom of the cryopreservation tube; the top of the carrier has an insertion hole, and the locking pin slides into the insertion hole; a second movable groove is provided inside the carrier; the second movable groove communicates with the insertion hole; an inverted metal locking cup is provided above the second movable groove, and the inner diameter of the cup bottom is smaller than that of the cup body, and the locking cup slides into the second movable groove; a first spring is provided inside the locking cup; the bottom of the locking cup has a through groove and several radial grooves arranged circumferentially with the through groove, the through groove communicates with the radial grooves respectively, and a locking ball is provided in the radial groove, the locking ball slides into the radial groove and the second movable groove respectively, wherein the side of the locking ball near the through groove intersects the extension line of the insertion hole in the length direction.

3. The donated brain in situ cryopreservation kit according to claim 1, characterized in that: The drive assembly includes a first electromagnet positioned below the mounting layer within each of the second partitions.

4. The donated brain in situ cryopreservation kit according to claim 3, characterized in that: The pressing assembly includes a first movable groove disposed within the top of the first enclosure; a movable block disposed within the first movable groove, the movable block being slidably engaged with the first movable groove; a second spring being fixedly connected between the bottom of the movable block and the bottom of the first movable groove; a connecting rod disposed on the side wall of the movable block, one end of the connecting rod being rotatably connected to the movable block, and the other end of the connecting rod being provided with a cylindrical protrusion; a touch switch disposed within the top of the movable block, the touch switch being electrically connected to an electromagnet; a limit protrusion and a stop block disposed on the side wall of the first movable groove, the limit protrusion being slidably engaged with the cylindrical protrusion, and the stop block being used to push the cylindrical protrusion back above the limit protrusion; wherein, the end of the partition cover away from the first movable groove is rotatably connected to the first enclosure, the bottom of the end of the partition cover near the first movable groove is provided with a first engaging protrusion, and the top of the movable block is also provided with a second engaging protrusion, the first engaging protrusion and the second engaging protrusion being engagedly connected.

5. The donated brain in situ cryopreservation kit according to claim 4, characterized in that: It also includes a second electromagnet, a display, and a controller located below the first movable slot; the display is used to receive information; the controller is used to determine which partition cover needs to be opened based on the received information; the controller is also electrically connected to the first electromagnet and the second electromagnet; wherein, the movable block is made of metal.

6. The donated brain in situ cryopreservation kit according to claim 5, characterized in that: The bottom of the second movable groove is also provided with a first spring groove, and the side wall of the first spring groove corresponds to the side wall of the locking cup.

7. The donated brain in situ cryopreservation kit according to claim 6, characterized in that: The slots are arranged along the gyri of the brain.

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