A method for promoting sunflower flowering under low light conditions

Abstract

The application provides a method for promoting sunflower flowering under weak light conditions, comprising the following steps: placing sunflower seedlings under weak light conditions for growth, spraying the sunflower seedlings with a sucrose solution, and stopping spraying the sucrose solution until the sunflower starts to show color, so as to obtain the sunflower in bloom. In the prior art, the sunflower cannot bloom or the flower diameter is small and deformed under the weak light conditions of a family environment and a southern region, and the sunflower cannot normally produce seeds. The application promotes the sunflower to bloom normally by spraying the sunflower with the sucrose solution, and can also advance the flowering period of the sunflower. The method is convenient, fast, easy to implement, low in cost, free of toxic side effects, and suitable for family gardening.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of plant flowering, in particular to a method for promoting sunflower flowering under weak light conditions. BACKGROUND

[0002] Sunflower (Helianthus annuus) is an annual herb of the Asteraceae family (Asteraceae) Helianthus genus (Helianthus), which is not only a major oil crop but also an important ornamental flower. Sunflower has excellent characteristics such as salt-tolerant, drought-tolerant, cold-resistant and strong adaptability, but it requires sufficient light and needs a light environment. The industrialization of sunflower planting in central and southern China is mainly limited by light conditions. Sunflower flowering is delayed and the disc diameter is smaller under weak light, and the yield is significantly reduced.

[0003] Potted sunflower is an important direction of family gardening development, but the weak light of family environment directly leads to the abnormal flowering of potted sunflower, which limits the industrial development of potted sunflower. Insufficient light is a key environmental factor affecting the development of sunflower industry, and the effect of light on the flowering period of sunflower is mainly divided into three aspects of photoperiod, light quality and light intensity. Most sunflower varieties are not sensitive to photoperiod, and can bloom under long-day and short-day conditions, but light quality and light intensity are the main light factors for the regulation of sunflower flowering period. Therefore, weak light intensity significantly affects sunflower flowering, and solving the problem of sunflower flowering under weak light conditions in family environment and southern region is crucial to the development of the industry. SUMMARY

[0004] The technical problem to be solved by the present application is how to promote sunflower flowering under weak light conditions.

[0005] The present application solves the above technical problems by the following technical means:

[0006] The present application provides a method for promoting sunflower flowering under weak light conditions, comprising the following steps: placing sunflower seedlings under weak light conditions for growth, spraying sucrose solution to the sunflower seedlings, stopping spraying sucrose solution until the sunflower starts to color, and obtaining flowering sunflower.

[0007] Beneficial effects: In the prior art, sunflower cannot bloom or the flower diameter becomes small and deformed under weak light conditions in family environment and southern region, and cannot normally produce seeds. The present application promotes sunflower to bloom normally by spraying sucrose solution, and can also advance the flowering period of sunflower. The method of the present application is convenient, fast, easy to implement, low in cost, has no toxic and side effects, and is suitable for family gardening.

[0008] Preferably, the light intensity of the weak light condition is 66-200 μmol·m-2 ·s -1 .

[0009] Preferably, the concentration of the sucrose solution is 50 mM.

[0010] Preferably, the spraying of the sucrose solution is performed every 3 days.

[0011] Preferably, the spraying method is spraying to the whole leaf.

[0012] Preferably, the sunflower seedling is a 12-day-old seedling after sowing.

[0013] Preferably, the growth condition is 16 hours of light and 8 hours of darkness, and the growth temperature is 25℃.

[0014] Preferably, the light intensity is 200 μmol·m -2 ·s -1 .

[0015] The advantage of the present application is that:

[0016] The present application promotes the flowering of sunflower under weak light by spraying sucrose solution to sunflower under weak light, and advances the flowering period of sunflower by 5-10 days. The method is simple and effective, and provides a theoretical basis and industrial guidance for the breeding and reasonable cultivation of sunflower under weak light. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 is the flowering period statistics and flowering phenotype of sunflower under different light intensities in the comparative example; A is the flowering period statistics of sunflower treated under different light intensities; B is the flowering phenotype of sunflower treated under different light intensities; C is the flowering phenotype of sunflower treated under light intensity of 66 μmol·m -2 ·s -1 .

[0018] Figure 2 is the flowering phenotype of sunflower treated with sucrose under different light intensities in the example and the comparative example;

[0019] Figure 3 is the flowering period statistics data of sunflower treated with sucrose under different light intensities in the example and the comparative example. DETAILED DESCRIPTION

[0020] In order to make the purposes, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be described clearly and completely below in conjunction with the embodiments of the present application. Obviously, the described embodiments are only some of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative effort belong to the scope of protection of the present application.

[0021] The test materials and reagents used in the following examples, and the like, can be obtained from commercial channels unless otherwise specified.

[0022] The specific techniques or conditions not specified in the examples can be carried out according to the techniques or conditions described in the literature in the art or according to the product instructions.

[0023] The sunflower seeds of the present application are ornamental sunflower'super smile' seeds, which are purchased from Xiamen Aikeng Gardening Company. The sunflower seeds are sowed in peat and germinated and grown in an artificial climate incubator under the condition of 66 μmol·m -2 ·s -1 The growth condition is 16 hours of light and 8 hours of darkness, and the growth temperature is 25℃. The sunflower seedlings are obtained after 12 days of growth.

[0024] Example 1

[0025] The present embodiment provides a method for promoting sunflower flowering under weak light conditions, which comprises the following steps: placing the above-mentioned sunflower seedlings in an artificial climate incubator under the condition of 66 μmol·m -2 ·s -1 Treating the sunflower seedlings with 50 mM sucrose solution, spraying the whole plant leaves, once every 3 days, until the sunflower starts to color, and stopping spraying the sucrose solution, to obtain the flowering sunflower.

[0026] Example 2

[0027] The present embodiment provides a method for promoting sunflower flowering under weak light conditions. The difference between the present embodiment and Example 1 is that the light intensity is different. The light intensity in the present embodiment is 200 μmol·m -2 ·s -1 .

[0028] Comparative Example 1

[0029] The present comparative example provides a method for sunflower flowering under normal conditions, which comprises the following steps: placing the above-mentioned sunflower seedlings in an artificial climate incubator under the condition of 360 μmol·m -2 ·s -1 , 200 μmol·m -2· ·s -1 and 66 μmol·m -2·s -1 The sunflowers were grown under different light intensities, sprayed with water, and their flowering period was observed and recorded.

[0030] Comparative Example 2

[0031] This comparative example provides a method for sunflower flowering. The difference between this comparative example and Example 1 is the light conditions; the light intensity in this comparative example is 360 μmol·m⁻¹. -2 ·s -1 .

[0032] Experimental example: The flowering period of sunflowers in Examples 1-2 and Comparative Examples 1-2 was recorded. The flowering period of sunflowers was calculated from the sowing date to the first flowering after the sunflowers showed color. The number of days was counted to obtain the flowering period of sunflowers.

[0033] Statistical analysis in Comparative Example 1 revealed that 360 μmol·m -2 ·s -1 Sunflowers under the specified light intensity conditions bloom first, with a flowering period earlier than those under 200 μmol·m light intensity. -2· s -1 The sunflowers were treated 3 days earlier, at 66 μmol·m -2 ·s -1 The flowering period of sunflowers treated with this method was significantly delayed compared to those treated with 200 μmol·m -2 ·s -1 The treated sunflowers bloomed 10 days later; statistical data can be found here. Figure 1 A. The flowering period of sunflowers treated under low light conditions was significantly delayed.

[0034] like Figure 1 As shown in BC, it grows at 66 μmol·m -2 ·s -1 Sunflowers under low light intensity conditions will exhibit a phenotype of not being able to bloom normally, with smaller and deformed flower diameters, and will not be able to produce seeds normally.

[0035] Comparing Examples 1-2 and Comparative Example 2, it was found that, Figures 2-3 As shown, at 360 μmol·m -2 ·s -1 After sucrose treatment under high light intensity conditions, the flowering time did not change significantly. At 200 μmol·m⁻¹, the flowering time remained the same. -2· ·s -1 Sunflowers treated with sucrose under low light conditions flowered 3 days earlier, at 66 μmol·m -2· ·s -1 Under low light conditions, sunflowers treated with sucrose bloomed 7 days earlier.

[0036] In conclusion, the sunflower flowering under weak light is promoted by the sucrose solution spraying treatment, and the flowering period of the sunflower is advanced by 3-7 days, the method is convenient, easy to implement, low in cost, free of toxic side effects, suitable for family gardening, and provides a theoretical basis and industrial guidance for sunflower breeding and reasonable cultivation under weak light.

[0037] The research found that the weak light plant flowering delay is partly due to the reduction of carbohydrates under weak light conditions caused by the change of juvenile period, because the plant can only complete the juvenile period to induce the outside environment to complete flowering, weak light prolongs the juvenile period or changes it into the juvenile period to change the flowering time, and exogenous sucrose can partially restore the weak light flowering phenotype.

[0038] Sugar is the main product of photosynthesis in many plants, which is transported from source tissues (such as mature leaves) to sink tissues such as shoot tips, flowers, seeds and roots through phloem to promote the development and growth of plants. The initiation of plant flower bud differentiation requires sufficient carbohydrates as energy supply, and the increase of sucrose provided by shoot apical meristem precedes the start of flower activation, and the induction of sucrose in the meristem to induce flower transition is related to light conditions, and long day or short day conditions can induce the content change of sucrose in the apical meristem. Plants assimilate carbon dioxide and water in vivo by photosynthesis to synthesize carbohydrates, which can be used as energy carriers to support their growth and development, and the reduction of light intensity will affect the carbon balance of crops and thus affect the growth and development of plants. Therefore, spraying sucrose solution under weak light conditions makes the sunflower carbon balance, so that the sunflower can flower under weak light and the flowering period is advanced.

[0039] The above examples are only used to illustrate the technical solutions of the present application, but not to limit it; although the present application has been described in detail with reference to the foregoing examples, those skilled in the art should understand that the technical solutions recorded in the foregoing examples can be modified, or some technical features can be replaced by equivalents; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. A method of promoting flowering of sunflower under low light conditions, characterized in that, The method comprises the following steps: The sunflower seedlings are placed in weak light conditions for growth, and a sucrose solution is sprayed thereon until the spraying of the sucrose solution is stopped when the sunflower begins to color, and a flowering sunflower is obtained; the light intensity of the weak light conditions is 66-200 μmol·m -2 ·s -1 ; and the concentration of the sucrose solution is 50 mM.

2. The method for promoting flowering of sunflower under low light conditions according to claim 1, characterized in that, The spraying of the sucrose solution is performed every 3 days.

3. The method of promoting flowering in sunflower under low light conditions as claimed in claim 1, wherein, The spraying is performed on the whole plant.

4. The method of promoting flowering in sunflower under low light conditions as claimed in claim 1, wherein, The sunflower seedlings are 12 days old.

5. The method of promoting flowering in sunflower under low light conditions as claimed in claim 1, wherein, The growth conditions are 16 hours of light and 8 hours of darkness, and the growth temperature is 25°C.

6. The method of promoting flowering in sunflower under low light conditions as claimed in claim 1, wherein, The light intensity is 200 pmol m -2 ·s -1 .

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