A wrinkle-resistant and skin-firming composition and its application in cosmetics

Abstract

The invention belongs to the field of cosmetics and relates to an anti-wrinkle and skin-firming composition and its application in cosmetics. The anti-wrinkle and skin-firming composition uses dipeptide diaminobutyryl benzylamide diacetate, acetylated sodium hyaluronate, Mirabilis jalapa extract and Glycine soja protein as main active ingredients, and achieves multiple effects of moisturizing, improving skin elasticity and anti-wrinkle under a reasonable mass ratio of (2-3): (5-7): 1: (8-10).

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and particularly relates to an anti-wrinkle and skin-firming composition and application thereof in cosmetics. Background Art

[0002] The skin consists of three layers: the epidermis, dermis, and subcutaneous tissue. The dermis contains a large amount of collagen and elastin, proteins that keep the skin elastic and firm. As we age, the production of these proteins decreases, causing the skin to lose elasticity and wrinkles. Moisture retention in the skin is essential to maintain its elasticity and smoothness. As we age, the skin's ability to retain moisture decreases, which can also lead to dry skin and the appearance of fine lines. Wrinkles are a natural phenomenon of skin aging, mainly caused by the following factors: Natural aging: As we age, the elasticity and collagen of the skin decrease, causing the skin to sag and wrinkles to form; UV exposure: Long-term exposure to UV rays can damage the skin and accelerate the skin aging process; Repetitive facial expressions: Frequently making the same expression, such as frowning or smiling, can cause expression lines on the skin; Bad lifestyle habits: Such as smoking, drinking, lack of sleep and poor diet, can affect skin health; Genetic factors: Some people may be more prone to wrinkles due to genetic reasons.

[0003] Modern research shows that enhanced moisturizing can maintain skin moisture and reduce fine lines caused by dryness; promoting collagen production, using products containing vitamin C, peptides and other ingredients can promote skin collagen production and increase skin elasticity; using products containing antioxidant ingredients such as vitamin E and green tea extract can neutralize free radicals and slow down skin aging; sunscreen components can also prevent ultraviolet damage to the skin and slow down skin aging.

[0004] Anti-wrinkle and skin-firming cosmetics are products specially designed to reduce or prevent skin wrinkles. These products usually contain ingredients that can improve skin elasticity, increase skin moisture, promote collagen production, or protect the skin from environmental damage. However, the anti-wrinkle and skin-firming cosmetics in the prior art are not effective, and the wrinkle improvement effect and skin firming effect are limited. In order to achieve a good anti-wrinkle and skin-firming effect, the present application provides an anti-wrinkle and skin-firming composition to improve the deficiencies of the prior art. Summary of the invention

[0005] The present invention provides an anti-wrinkle and skin-firming composition and application thereof in cosmetics, and rationally compounding the raw material components to achieve the multiple effects of moisturizing, improving skin elasticity and anti-wrinkle.

[0006] The first aspect of the present invention is to provide an anti-wrinkle and skin firming composition comprising the following ingredients:

[0007] Dipeptide diaminobutyroyl benzylamide diacetate, sodium acetylated hyaluronate, Mirabi LISJALAPA extract and Glycine Soja protein.

[0008] In one embodiment, the mass ratio of dipeptide diaminobutyryl benzylamide diacetate, sodium acetylated hyaluronate, Mirabilis jalapa extract and Glycine soja protein is (0.5-4):(1-10):(0.1-1.5):(1-10).

[0009] In one embodiment, the mass ratio of dipeptide diaminobutyryl benzylamide diacetate, sodium acetylated hyaluronate, Mirabilis jalapa extract and Glycine soja protein is (1-4): (3-8): (0.5-1.2): (5-10).

[0010] In one embodiment, the mass ratio of dipeptide diaminobutyryl benzylamide diacetate, sodium acetylated hyaluronate, Mirabilis jalapa extract and Glycine soja protein is (2-3):(5-7):1:(8-10).

[0011] The second aspect of the present invention is to provide an anti-wrinkle and skin-firming cosmetic, including an anti-wrinkle and skin-firming composition.

[0012] In one embodiment, the anti-wrinkle and skin firming composition comprises the following ingredients: dipeptide diaminobutyryl benzylamide diacetate, sodium acetylated hyaluronate, Mirabilis jalapa (MIRABILIS JALAP A) extract and Glycine soja (Glycine soja) protein.

[0013] In one embodiment, the mass ratio of dipeptide diaminobutyryl benzylamide diacetate, sodium acetylated hyaluronate, Mirabilis jalapa extract and Glycine soja protein is (0.5-4):(1-10):(0.1-1.5):(1-10).

[0014] In one embodiment, the mass ratio of dipeptide diaminobutyryl benzylamide diacetate, sodium acetylated hyaluronate, Mirabilis jalapa extract and Glycine soja protein is (1-4): (3-8): (0.5-1.2): (5-10).

[0015] In one embodiment, the mass ratio of dipeptide diaminobutyryl benzylamide diacetate, sodium acetylated hyaluronate, Mirabilis jalapa extract and Glycine soja protein is (2-3):(5-7):1:(8-10).

[0016] In one embodiment, the anti-wrinkle and skin-firming cosmetic further comprises one or more of a moisturizer, an emulsifier, a thickener, a chelating agent, a preservative, a pH adjuster, a colorant, a fragrance, and deionized water.

[0017] In one embodiment, the humectant may be selected from one or more of glycerin, butylene glycol, polyethylene glycol, propylene glycol, xylitol, urea and panthenol.

[0018] In one embodiment, the emulsifier can be selected from one or more of C12-20 alkyl glucoside, acrylates / C10-30 alkyl acrylate crosspolymer, steareth-2, polyglyceryl fatty acid esters, isononyl isononanoate, C14-22 alcohol, cetearyl alcohol, cetearyl glucoside, shea butter, dimethicone and polysorbate-60.

[0019] In one embodiment, the thickener may be selected from one or more of xanthan gum, carbomer, cellulose, carrageenan, gellan gum and polyacrylic acid copolymers.

[0020] In one embodiment, the chelating agent may be selected from disodium EDTA or tetrasodium EDTA.

[0021] In one embodiment, the preservative may be selected from one or more of phenoxyethanol, p-hydroxyacetophenone, methylparaben, propylparaben and ethylhexylglycerin.

[0022] In one embodiment, the pH adjuster may be selected from one or more of citric acid, sodium dihydrogen phosphate, sodium bicarbonate, sodium hydroxide, and triethanolamine.

[0023] In one embodiment, an anti-wrinkle and skin-firming cosmetic comprises: an anti-wrinkle and skin-firming composition, a moisturizer, an emulsifier, a thickener, a preservative and deionized water;

[0024] The anti-wrinkle and skin firming composition comprises the following ingredients: dipeptide diaminobutyryl benzylamide diacetate, acetylated sodium hyaluronate, Mirabilis jalapa extract and Glycine soja protein.

[0025] In one embodiment, the mass ratio of dipeptide diaminobutyryl benzylamide diacetate, sodium acetylated hyaluronate, Mirabilis jalapa extract and Glycine soja protein is (1-4): (3-8): (0.5-1.2): (5-10).

[0026] In one embodiment, the mass ratio of dipeptide diaminobutyryl benzylamide diacetate, sodium acetylated hyaluronate, Mirabilis jalapa extract and Glycine soja protein is (2-3):(5-7):1:(8-10).

[0027] In one embodiment, the anti-wrinkle and skin-firming cosmetic comprises, by mass percentage: 5-12% of the anti-wrinkle and skin-firming composition, 8-15% of a moisturizer, 3-10% of an emulsifier, 0.1-1% of a thickener, 0.01-0.1% of a preservative, and the balance of deionized water;

[0028] The anti-wrinkle and skin firming composition comprises the following ingredients: dipeptide diaminobutyryl benzylamide diacetate, acetylated sodium hyaluronate, Mirabilis jalapa extract and Glycine soja protein.

[0029] In one embodiment, the mass ratio of dipeptide diaminobutyryl benzylamide diacetate, sodium acetylated hyaluronate, Mirabilis jalapa extract and Glycine soja protein is (2-3):(5-7):1:(8-10).

[0030] The third aspect of the present invention is to provide use of the anti-wrinkle and skin-firming composition in the preparation of cosmetics.

[0031] In one embodiment, the cosmetic may be selected from cleansing milk, cleansing cream, toner, lotion, emulsion, cream, essence, facial mask, eye cream, eye mask and body lotion.

[0032] Compared with the prior art, the beneficial technical effects of the present invention are:

[0033] The anti-wrinkle and skin-firming composition of the present invention rationally compounds the raw material components of dipeptide diaminobutyryl benzylamide diacetate, acetylated sodium hyaluronate, Mirabilis jalapa extract and Glycine soja protein, and adjusts the dosage ratio multiple times, so that the dipeptide diaminobutyryl benzylamide diacetate, acetylated sodium hyaluronate, Mirabilis jalapa extract and Glycine soja protein can exert their maximum effect under the condition of a mass ratio of (2-3): (5-7): 1: (8-10). Effective experiments have confirmed that the composition has good moisturizing and hydrating effects, significantly improves the elasticity of the skin at the corners of the eyes and increases the elastin inhibition rate. The multiple effects are combined to achieve the anti-wrinkle and skin-firming effects. In addition, the composition is non-irritating, highly safe, and suitable for a wide range of people.

[0034] Instruction Manual

[0035] Figure 1 Moisturizing effect test results chart DETAILED DESCRIPTION

[0036] In order to more fully understand the technical content of the present invention, the present invention will be further introduced and illustrated in conjunction with specific embodiments below; obviously, the embodiments described below are only part of the embodiments of the present invention, rather than all of the embodiments; based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without making creative work are within the scope of protection of the present invention.

[0037] The raw materials used in the embodiment are all conventional commercially available products.

[0038] The main raw materials involved in the implementation method are as follows:

[0039] Dipeptide diaminobutyryl benzylamide diacetate is a neuropeptide. Its cosmetic effect mimics the snake venom toxin Waglerinl. It competes with acetylcholine for receptors on the muscle cell membrane, preventing acetylcholine from binding to the muscle cell, making the muscle unable to contract and in a relaxed state, thus achieving the cosmetic purpose of smoothing wrinkles. Compared with botulinum toxin, dipeptide diaminobutyryl benzylamide diacetate does not require injection, which is safer and more economical. It is suitable for facial expression muscle parts, such as the forehead and around the eyes, targeting crow's feet, nasolabial folds, expression lines, and Sichuan-shaped lines.

[0040] Acetylated sodium hyaluronate is obtained by acetylation of the natural moisturizing factor sodium hyaluronate (HA). Studies have shown that acetylated sodium hyaluronate can prevent hyaluronidase degradation, thereby penetrating the skin layer better and deeper. The introduction of acetyl groups makes acetylated sodium hyaluronate both hydrophilic and lipophilic, and can play a role in double moisturizing, repairing the keratin barrier, improving skin elasticity and other biologically active functions, thereby improving dry and rough skin conditions and making the skin soft and elastic.

[0041] Mirabilis jalapa extract, Mirabilis jalapa is a perennial herbaceous flower of the Mirabilis genus of the Mirabilis family, and its whole plant extract is often used in cosmetics. Mirabilis jalapa extract can stimulate the secretion of key factors for epidermal synthesis of ceramide, occludin, and hyaluronic acid, improve skin barrier function and water-locking ability, reduce inflammation and calm the skin, reduce facial flushing, and can be used as an antioxidant, moisturizer, and anti-inflammatory agent, and can be used to prepare repair and moisturizing products.

[0042] Glycine Soja protein is extracted from wild soybeans and contains soluble high molecular weight components of soybean plant matrix, which can enhance the skin collagen metabolism activity and whitening effect, allowing the skin to repair itself. It can promote the growth of fibroblasts, has the effect of skin revitalization and anti-aging; at the same time, it can maintain the moisture of the skin and has a moisturizing effect.

[0043] The anti-wrinkle and firming skin essences of Examples 1-3 were prepared according to the mass percentages in Table 1.

[0044] Table 1: Formula of the anti-wrinkle and firming skin essence of Examples 1-3

[0045]

[0046]

[0047] The preparation method of the anti-wrinkle and firming skin serum is as follows:

[0048] S1. Accurately weigh the raw materials and set aside;

[0049] S2, put the Mirabilis jalapa extract, C12-20 alkyl glucoside, acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer into the first stirring pot, set the temperature to 80 ° C, the stirring speed to 1800 rpm, the stirring time to 40 min, stir evenly, cool to room temperature, and obtain material A;

[0050] S3, put acetylated sodium hyaluronate, glycerol, butylene glycol and deionized water into a second stirring pot, set the temperature to 40°C, the stirring speed to 1200 rpm, the stirring time to 20 min, stir evenly to obtain material B;

[0051] S4. Add material B obtained in step S3 to material A obtained in step S2 in portions, stir evenly, then add dipeptide diaminobutyryl benzylamide diacetate, soybean (Glycine Soja) protein, xanthan gum, and phenoxyethanol in sequence, stir at 45° C. and 2000 rpm for 45 min, stir evenly, and obtain the product.

[0052] The anti-wrinkle and firming skin essences of Comparative Examples 1-4 were prepared according to the mass percentages in Table 2.

[0053] Table 2 Formula of the anti-wrinkle and firming skin essence of Comparative Examples 1-4

[0054]

[0055]

[0056] Comparative Example 5

[0057] The only difference from Example 2 is that hydrolyzed hyaluronic acid is used to replace acetylated sodium hyaluronate.

[0058] Comparative Example 6

[0059] The only difference from Example 2 is that the Glycine Soja (Glycine Soja) seed extract is used to replace the Glycine Soja (Glycine Soja) protein.

[0060] Comparative Example 7

[0061] The only difference from Example 2 is that the Mirabilis jalapa extract is replaced with the Jasminum sambac flower extract.

[0062] The preparation method of the comparative example is the same as that of the embodiment.

[0063] 1. Skin occlusive patch test

[0064] With reference to the "Technical Specifications for Safety of Cosmetics" (2015 edition), 33 female testers were recruited to detect the potential possibility of the essences of Examples 1-3 and Comparative Examples 1-7 causing adverse reactions to human skin, and a blank control group was set up with deionized water. The essences or deionized water of Examples 1-3 and Comparative Examples 1-7 were placed in the small chamber of the spot tester, respectively, with an amount of about 0.02 mL. The spot tester with the sample was applied to the curved side of the left forearm of the tester, and the palm of the hand was gently pressed to evenly apply it to the skin for 24 hours. After removing the test spot tester, observe the skin reaction according to the standards in Table 3 30 minutes, 24 hours, and 48 hours, and record the results.

[0065] Table 3 Grading criteria for adverse skin reactions

[0066]

[0067]

[0068] The 24-hour skin occlusive patch test showed that after removing the patch tester for 30 minutes, 24 hours, and 48 hours, a total of 33 testers in the blank control group, Example 1-3 groups, and Comparative Example 1-7 groups all had negative reactions. The test showed that the essence containing the anti-wrinkle and skin firming composition of the present invention will not cause adverse reactions to the skin and has good safety.

[0069] 2. Hydrating and moisturizing effect test

[0070] Referring to the cosmetic moisturizing efficacy test method of the "Guidelines for Evaluation of Cosmetic Moisturizing Efficacy" (QB / T 4256-2011), 33 female testers aged 25 to 35 were included. In an environment with a temperature of 20 to 22°C and a humidity of 40 to 60%, the skin moisture content index test was conducted on the inside of the right arm of the testers using a skin moisture tester Corneometer CM825. The unit of skin moisture content is AU (Arbitrary Units). A blank control group was set up. The sample used in the blank control group was a matrix liquid that did not contain an anti-wrinkle and firming skin composition. Its mass percentage composition was: 6% glycerin, 4% butylene glycol, 3% C12-20 alkyl glucoside, 6% acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer, 0.3% xanthan gum, 0.1% phenoxyethanol, and the balance was deionized water. The application amount was about 0.5mL, the application area was 5cm*5cm, and the test time points were before use and 4h after use. The test results are as follows: Figure 1 .

[0071] The test results show that the anti-wrinkle and firming skin essences of Examples 1-3 can increase the moisture content of the skin compared with Comparative Examples 1-7, and the moisturizing effect is significant. Compared with before use, the skin moisture content of Examples 1-3 increased by 24.44% (28.85±1.71 vs 35.90±2.33), 29.49% (27.91±3.02 vs 36.14±1.32), and 30.95% (28.04±2.67 vs 36.72±1.88) 4 hours after use, respectively, proving that the anti-wrinkle and firming skin composition of the present invention produces unexpected beneficial effects through reasonable composition and dosage ratio.

[0072] 3. Eye corner skin elasticity test

[0073] 43 healthy female testers aged 35 to 45 years old were selected, and the test was carried out in an environment with a temperature of 20 to 22 ° C, a humidity of 40 to 60%, natural light, and avoiding direct sunlight. No cosmetics were applied to the test site 8 hours before the test. A blank control group was set up, and the sample used in the blank control group was a matrix liquid that did not contain an anti-wrinkle and firming skin composition, and its mass percentage composition was: 6% glycerol, 4% butylene glycol, 3% C12-20 alkyl glucoside, 6% acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer, 0.3% xanthan gum, 0.1% phenoxyethanol, and the balance of deionized water. The anti-wrinkle and firming skin essence or matrix liquid prepared in the above-mentioned embodiments 1-3 and comparative examples 1-7 were applied to the left and right peripheries of the tester, respectively, with an amount of about 0.5 mL per side, and gently massaged until completely absorbed. Four testers were set in each group of embodiments 1-3 and comparative examples 1-7, and three testers were set in the blank control group. 4 hours after application, the skin elasticity of the eye corners was measured using a skin elasticity tester (Cutometer MPA 580), and each part was tested 3 times to take the average value. The test results were expressed by the ratio R2 of the skin rebound amount Ua without negative pressure to the maximum stretch amount Uf of the skin, and the ratio R7 of the elastic part Ur of the skin recovery process to the maximum stretch amount Uf of the skin. The closer R2 and R7 are to 1, the better the skin elasticity. The test results are shown in Table 4.

[0074] Table 4 Eye corner skin elasticity test results

[0075]

[0076]

[0077] Skin elasticity can reflect the degree of skin aging. The lower the skin elasticity parameter value, the worse the skin elasticity. Among various parts of the face, the skin elasticity of the corners of the eyes is more significantly affected by age, ultraviolet rays, etc. The skin elasticity test results using the corners of the eyes as the test site show that the anti-wrinkle and firming skin essences of Examples 1-3 can improve the elasticity of the skin at the corners of the eyes and significantly improve the firmness of the skin compared to Comparative Examples 1-7, thereby confirming that the anti-wrinkle and firming skin composition of the present invention produces unexpected beneficial effects through reasonable composition and dosage ratio.

[0078] 4. Elastase inhibition rate test

[0079] The experimental principle of inhibiting elastase is that porcine pancreatic elastase (PPE) type IV catalyzes a reaction with the substrate n-succ-(Ala)3-Pnitroanilide (SANA). After adding the active substance, the absorbance changes. The magnitude of the absorbance change reflects the inhibition rate of the elastase inhibitor, thereby screening the elastase inhibitor.

[0080] Test method: Take 10 μg / mL active porcine pancreatic elastase (PPE) type IV, 5×10 -3 mol / L substrate n-succ-(Ala)3-Pnitroanilide (SANA), anti-wrinkle and firming skin composition AJ (see Table 5) with a final concentration of 5% v / v, reacted at 25°C, pH=8 for 10 minutes, measured absorbance at 410nm, analyzed and processed data to calculate the inhibition rate of elastase of the sample to be tested, and the test results are shown in Table 6. Deionized water was set as a blank control group. The test method refers to "'Evaluation Method of Anti-Wrinkle Efficacy of Cosmetics', Liu Yang, Deng Yingmei, Zhao Hua, Daily Chemicals Science, Vol. 38, No. 4, April 2015".

[0081] Table 5 Anti-wrinkle and skin firming composition AG

[0082]

[0083] Composition H: The only difference from composition B is that the acetylated sodium hyaluronate is replaced by an equal amount of hydrolyzed hyaluronic acid.

[0084] Composition I: The only difference from composition B is that the Glycine Soja (Glycine Soja) protein is replaced with an equal amount of Glycine Soja (Glycine Soja) seed extract.

[0085] Composition J: The only difference from composition B is that the Mirabilis jalapa extract is replaced with an equal amount of Jasminum sambac flower extract.

[0086] Table 6 Elastase inhibition rate

[0087]

[0088]

[0089] Elastase inhibitors can effectively inhibit the activity of elastase, slow down the degradation rate of elastin, and play a role in delaying aging and reducing wrinkles. The test results show that the anti-wrinkle and firming skin composition AC can more effectively inhibit elastase than the anti-wrinkle and firming skin composition DJ, and there is a significant difference. The test results once again confirm that the anti-wrinkle and firming skin composition of the present invention produces unexpected beneficial effects through reasonable composition and dosage ratio.

[0090] The above contents are further detailed descriptions of the present invention in combination with specific preferred embodiments, and it cannot be determined that the specific implementation of the present invention is limited to these descriptions. For ordinary technicians in the technical field to which the present invention belongs, several simple deductions or substitutions can be made without departing from the concept of the present invention, which should be regarded as falling within the protection scope of the present invention.

Claims

1. A composition for anti-wrinkle and skin firming, comprising the following ingredients: Dipeptide diaminobutyroyl benzylamide diacetate, sodium acetylated hyaluronate, Mirabilis jalapa extract and Glycine soja protein; The mass ratio of dipeptide diaminobutyryl benzylamide diacetate, sodium acetylated hyaluronate, Mirabilis jalapa extract and Glycine soja protein is (1-4): (3-8): (0.5-1.2): (5-10); The mass percentage of the anti-wrinkle and skin-firming composition in the cosmetics is 5-12%.

2. The composition according to claim 1, characterized in that The mass ratio of dipeptide diaminobutyryl benzylamide diacetate, sodium acetylated hyaluronate, Mirabilis jalapa extract and Glycine soja protein is (2-3):(5-7):1:(8-10).

3. An anti-wrinkle and skin-firming cosmetic, characterized in that: A composition for anti-wrinkle and skin firming comprising the composition according to any one of claims 1 to 2.

4. The cosmetic according to claim 3, characterized in that: The composition comprises, by mass percentage, 5-12% of the anti-wrinkle and skin-firming composition, 8-15% of a moisturizer, 3-10% of an emulsifier, 0.1-1% of a thickener, 0.01-0.1% of a preservative and the balance of deionized water.

5. Use of the anti-wrinkle and skin-firming composition according to any one of claims 1 to 2 in the preparation of cosmetics.

6. The use according to claim 5, characterized in that The cosmetic may be selected from cleansing milk, cleansing cream, toner, lotion, emulsion, cream, essence, facial mask, eye cream, eye mask and body lotion.

Images