Application of Fuling Gancao Decoction in the preparation of drugs for treating hyperlipidemia

Abstract

The present application relates to a new use of Fuling Gancao Decoction, and specifically discloses application of Fuling Gancao Decoction in treatment of hyperlipidemia, wherein the Fuling Gancao Decoction is composed of the following components in parts by weight: Fuling 2 parts, Guizhi 2 parts, Zhigancao 1 part and Shengjiang 3 parts. It is found through animal experiment research that the Fuling Gancao Decoction can improve the symptoms of hyperlipidemia rats, reduce the content of TG, TC and LDL-C in serum, improve the liver lipid, blood sugar of hyperlipidemia model animals, reduce the liver coefficient, improve the liver cell fatty degeneration, reverse the pathological morphology of adipose tissue cells and the mitochondrial damage of hyperlipidemia model animals. A new indication of the Fuling Gancao Decoction is found, the development of traditional Chinese medicines is promoted, and a new drug composition with less side effects is provided for treatment of hyperlipidemia.

Description

Technical Field

[0001] This invention relates to a new use of Fuling Gancao Decoction, specifically its application in the treatment of hyperlipidemia. Background Technology

[0002] The statements in this section provide only background information relevant to the disclosure of this application and may not constitute prior art.

[0003] Poria and Licorice Decoction, originating from *Treatise on Cold Damage*, has the effects of warming the middle jiao, resolving phlegm, promoting yang, and facilitating urination. It is primarily used to treat phlegm retention in the epigastrium, palpitations, sweating without thirst, dysuria, cough with urinary incontinence, and abdominal distension. *Treatise on Cold Damage* records its indications as: typhoid fever with sweating and no thirst; typhoid fever with syncope and palpitations. *General Records of Sacred Relief* records its indications as: typhoid fever followed by abdominal distension and dysuria after sweating. *Universal Relief Prescriptions*, quoting *Direct Guide*, records its indications as: phlegm retention in the epigastrium and palpitations. *Summary of Internal Medicine* records its indications as: cough in the bladder and urinary incontinence. *Compendium of Hernia and Abdominal Masses* records its indications as: hernia with abdominal distension.

[0004] There is currently no publicly available information regarding its use in treating hyperlipidemia.

[0005] Properties of Poria: Poria is a commonly used spleen-strengthening and dampness-eliminating herb, possessing the effects of promoting diuresis, eliminating dampness, strengthening the spleen, and calming the mind. It is often used to treat symptoms such as edema, difficulty urinating, spleen deficiency with poor appetite, palpitations, and insomnia.

[0006] Ginger properties: Dried ginger has the effects of relieving exterior syndromes and dispelling cold, warming the middle jiao and stopping vomiting, warming the lungs and stopping cough, and detoxifying. It is mainly used to treat colds due to wind-cold, spleen and stomach cold syndrome, vomiting due to stomach cold, and cough due to lung cold.

[0007] Properties of Prepared Licorice Root: Prepared licorice root is a common harmonizing herb, possessing the effects of tonifying the spleen and replenishing qi, and restoring the pulse. It is often used to harmonize the properties of other herbs, alleviate the toxic side effects of drugs, and treat spleen and stomach weakness, fatigue, palpitations, and irregular pulse.

[0008] Cinnamon twig properties: Cinnamon twig has the effects of inducing sweating and relieving muscle tension, warming the meridians and promoting blood circulation. It is often used to treat symptoms such as colds caused by wind and cold, joint pain, and irregular menstruation. Cinnamon twig can also help dilate blood vessels and improve blood circulation.

[0009] The principle of "principal, assistant, adjuvant, and guide" in the medicinal materials used in this application is as follows:

[0010] Poria cocos, with a bland taste and neutral nature, enters the heart, spleen, lung, and kidney meridians. It can strengthen the spleen, promote diuresis, and eliminate dampness. Zhang Xichun of the Qing Dynasty said in "Medical Records of Integrating Chinese and Western Medicine" that it "is good at regulating the spleen and stomach, because the spleen and stomach belong to earth, and its bland taste can nourish the spleen yin." "Its function is to transform phlegm and fluid in the stomach into water, guide it to the spleen and then to the lungs, and then descend along the water passages of the triple burner to the bladder. It is the main medicine for eliminating dampness and eliminating phlegm." Poria cocos strengthens the spleen and promotes diuresis, combining elimination and tonification, and promotes diuresis and warms yang, so it is the chief medicine.

[0011] Cinnamon twig, pungent in taste and warm in nature, enters the heart, lung, and bladder meridians. It can warm and unblock the meridians, and promote yang and qi circulation. Wang Tailin of the Qing Dynasty wrote in his *Annotations to the Collection of Prescriptions from Retreat and Reflection*: "Why is cinnamon twig always used as an adjuvant? Because the kidney water in the lower burner must be accompanied by liver evil to rise upwards. Only cinnamon can cut down the wood element, and it can also support yang and dispel cold, so it must be used." Therefore, it is an assistant herb. Cinnamon twig warms yang and promotes qi circulation, while Poria cocos strengthens the spleen, promotes diuresis, and eliminates dampness. The combination of cinnamon twig and Poria cocos achieves the effects of warming yang, resolving phlegm, promoting qi circulation, and promoting diuresis.

[0012] Ginger, pungent in flavor and slightly warm in nature, enters the lung, spleen, and stomach meridians. It moves without lingering, warming the stomach yang and dispersing dampness, thus serving as an adjuvant. This formula heavily utilizes ginger, which, when combined with poria and cinnamon twig, warms the stomach yang and disperses dampness.

[0013] Prepared licorice root is sweet in taste and warm in nature. It enters the heart, lung, spleen, and stomach meridians. It tonifies the spleen, replenishes qi, resolves phlegm, harmonizes other herbs, and serves as an adjuvant herb. When combined with poria, cinnamon twig, and ginger, it works synergistically to warm yang, promote qi circulation, and resolve phlegm. Summary of the Invention

[0014] The purpose of this invention is to address the current limitation of drugs for treating hyperlipidemia by providing the application of Fuling Gancao Decoction in the treatment of hyperlipidemia, and to provide a new drug composition for treating hyperlipidemia. At the same time, Fuling Gancao Decoction is a traditional Chinese herbal medicine, which has the advantage of fewer side effects compared with Western medicine treatment.

[0015] The technical solution of the present invention is as follows:

[0016] This invention provides the application of Poria and Licorice Decoction in the treatment of hyperlipidemia. The Poria and Licorice Decoction is composed of the following ingredients in parts by weight: 2 parts Poria, 2 parts Cinnamon Twig, 1 part Prepared Licorice Root, and 3 parts Fresh Ginger.

[0017] According to a preferred embodiment, the Poria and Licorice Decoction uses a compound water extract.

[0018] According to a preferred embodiment, the method for preparing the water extract is as follows:

[0019] Step S1: First decoction; Weigh each substance according to the weight parts of claim 1, add 8-10 times the weight of water, soak for 30-60 minutes, then heat to 100°C, maintain at 100°C for 1-1.5 hours, filter with gauze to obtain the first decoction residue and the first decoction aqueous solution.

[0020] Step S2: Second decoction: Put the dregs from the first decoction into the pot, add 8-10 times the weight of water, heat to 100℃, keep at 100℃ for 30-60 minutes, filter to obtain the second decoction dregs and the second decoction liquid.

[0021] Step S3: Mix the first decoction and the second decoction to obtain the compound water extract.

[0022] According to a preferred embodiment, the Poria and Licorice Decoction reduces serum TG, TC and LDL-C to treat hyperlipidemia.

[0023] According to a preferred embodiment, the Poria and Licorice Decoction improves liver lipids and blood glucose in hyperlipidemic model animals and reduces liver coefficient to treat hyperlipidemia.

[0024] According to a preferred embodiment, the Poria and Licorice Decoction improves hepatic steatosis and treats hyperlipidemia.

[0025] According to a preferred embodiment, the Poria and Licorice Decoction reverses the pathological morphology of adipose tissue cells and mitochondrial damage in a hyperlipidemia model animal to treat hyperlipidemia.

[0026] Compared with existing technologies, the advantages of this invention are:

[0027] 1. Application of Poria and Licorice Decoction in the treatment of hyperlipidemia. Poria and Licorice Decoction was originally used to treat symptoms related to wind-cold. The inventors of this application have discovered that it has a better therapeutic effect on hyperlipidemia, opening up a new path for the treatment of hyperlipidemia and providing a new Chinese medicine compound with fewer side effects compared to Western medicine.

[0028] 2. Application of Fuling Gancao Decoction in the treatment of hyperlipidemia: Animal experiments have verified that Fuling Gancao Decoction can reduce serum TG, TC and LDL-C, improve liver lipids and blood glucose in hyperlipidemic model animals, and reduce liver coefficient, thus it can treat hyperlipidemia.

[0029] 3. Application of Fuling Gancao Decoction in the treatment of hyperlipidemia: Animal experiments have verified that Fuling Gancao Decoction can also improve hepatocyte steatosis, reverse the pathological morphology of adipose tissue cells and mitochondrial damage in hyperlipidemia model animals, restore the normal function of liver cells, and treat hyperlipidemia. Attached Figure Description

[0030] Figure 1 The data are the serum biochemical parameters of SD rats in each experimental group in Example 1;

[0031] Figure 2 The data are the liver biochemical indicators of SD rats in each experimental group in Example 1;

[0032] Figure 3 This is an observation of the morphological characteristics of liver tissue cells from SD rats in each experimental group in Example 1.

[0033] Figure 4 Morphological observation of adipocytes in the white adipose tissue of SD rats in each experimental group in Example 1;

[0034] Figure 5Morphological observation of adipocytes in brown adipose tissue of SD rats in each experimental group in Example 1;

[0035] Figure 6 Data graph of the proportion of adipose tissue of SD rats in each experimental group in Example 1;

[0036] Figure 7 Data graph of lipid accumulation observed by Oil Red O staining of liver tissue of SD rats in each experimental group in Example 1;

[0037] Figure 8 Effect of Poria and Licorice Decoction on the morphology of adipocytes in white adipose tissue and mitochondrial damage in the cytoplasm; in the figure, Mi is mitochondria; the red arrow indicates adipocytes with obvious dilation of the endoplasmic reticulum; N is the nucleus, the green arrow indicates collagen fibers, the yellow arrow indicates ribosome loss, and the purple arrow indicates basement membrane rupture;

[0038] Figure 9 Effect of Poria and Licorice Decoction on the morphology of adipocytes in brown adipose tissue and mitochondrial damage in the cytoplasm, Mi is mitochondria, LD is lipid droplet; the red arrow indicates adipocytes with swollen mitochondria; the yellow arrow indicates autophagy. Detailed implementation mode

[0039] It should be noted that the test methods in the following examples are all conventional methods unless otherwise specified. The test materials used in the following examples are all commercially available.

[0040] The characteristics and performance of the present invention will be further described in detail below in conjunction with the examples.

[0041] Example 1 Pharmacodynamic evaluation of Poria and Licorice Decoction in the treatment of hyperlipidemia

[0042] I. Experimental materials

[0043] 1.1 Animals

[0044] SD rats, male, SPF grade, body weight 200 - 220 g, provided by Spf (Beijing) Biotechnology Co., Ltd., animal production license number: SCXK (Beijing) 2019 - 0010; the feeding environment is at a temperature of 18 - 22 °C, humidity of 50% - 60%, and 12 - hour alternating lighting. The animal experiment was reviewed and approved by the Ethics Committee of the Animal Experiment Center of Yunnan University of Traditional Chinese Medicine, approval number: R - 062022121.

[0045] 1.2 Drugs and reagents

[0046] Fresh ginger and Poria cocos were purchased from the Traditional Chinese Medicine Slices Factory of Yunnan Fulintang Pharmaceutical Co., Ltd. (item numbers 020636 and 020600); prepared licorice root was purchased from Yunnan Zongshun Biotechnology Co., Ltd. (batch number C240221004); and cinnamon twig was purchased from Hongya County Wawushan Pharmaceutical Co., Ltd. (batch number 220601). Triglycerides (TG; item number: A111-1-1), total cholesterol (TC; item number: A110-1-1), high-density lipoprotein (HDL; item number: A112-1-1), and low-density lipoprotein (LDL) were also tested. The following reagents were purchased: Lipoprotein (LDL) test kit (Catalog No.: A113-1-1) from Nanjing Jiancheng Biomedical Engineering Institute; PRDM16 and PGC-1α (Catalog Nos.: DF13303 and AF5359); UCP1 antibody from Wuhan Sanying Biotechnology Co., Ltd. (Catalog No.: 23673-1-AP); Anhydrous ethanol (Catalog No.: 100092683), xylene (Catalog No.: 10023418), and neutral resin (Catalog No.: 10004160) from Sinopharm Chemical Reagent Co., Ltd.; Environmentally friendly dewaxing solution (Catalog No.: G1128), universal tissue fixative (Catalog No.: G1101), and HE staining solution kit (Catalog No.: G1003) from Wuhan Saive Biotechnology Co., Ltd.; and sodium pentobarbital from Merck AG, Germany, batch No.: P3761.

[0047] 1.3 Instruments

[0048] SQP electronic analytical balance, Sartorius GmbH, Germany; MILLI-Q ultrapure water system, Millipore Corporation, USA; DK-S24 electric thermostatic water bath, Shanghai Jinghong Experimental Equipment Co., Ltd.; FMB-40 fully automatic snowflake ice maker, Shanghai Bilang Instrument Manufacturing Co., Ltd.; EXF24086V ultra-low temperature freezer, Thermo Fisher Scientific Ltd.; SB-4200 ultrasonic cleaner, Ningbo Xinzhi Biotechnology Co., Ltd.; HR / 16M high-speed refrigerated centrifuge, Hunan Hexi Instrument Equipment Co., Ltd.

[0049] Plus384 microplate reader (Molecular Devices, USA); N-11000-WD rotary evaporator (Shanghai Ailang Instrument Co., Ltd.); FD5-3 freeze dryer (KingSimon International Group, USA); XS-900i fully automated blood analyzer (Huaxin Technology Co., Ltd.); RM2016 pathology slider (Leica Instruments, Shanghai); Donatello dehydrator (DIAPATH, Italy); JB-P5 embedding machine (Wuhan Junjie Electronics Co., Ltd.); JB-L5 freezing stage (Wuhan Junjie Electronics Co., Ltd.); KD-P tissue spreader (Jinhua Kedi Instrument Equipment Co., Ltd., Zhejiang Province); CRYOSTAR NX50 cryostat (Thermo Fisher Scientific (China) Co., Ltd.); NIKON ECLIPSE E100 upright optical microscope and NIKON DS-U3 imaging system (Nikon Corporation, Japan).

[0050] II. Experimental Methods and Results

[0051] (1) Preparation of Poria and Licorice Decoction Extract

[0052] Extraction and quality control of the compound formula of Poria and Licorice Decoction. Step S1: First decoction; weigh each substance according to the ratio of Poria: Cinnamon Twig: Prepared Licorice Root: Fresh Ginger of 2:2:1:3 by weight, add 10 times the weight of water, soak for 30 minutes, then heat to 100℃ and maintain at 100℃ for 1 hour. Filter with gauze to obtain the first decoction residue and the first decoction solution.

[0053] Step S2: Second decoction: Put the dregs from the first decoction into the pot, add 8 times the weight of water, heat to 100℃, keep at 100℃ for 30 minutes, filter to obtain the second decoction dregs and the second decoction liquid.

[0054] Step S3: Mix the first decoction and the second decoction to obtain the compound water extract.

[0055] After freeze-drying, the yield of the compound aqueous extract is calculated to facilitate the conversion of the dosage for rat administration.

[0056] (2) Evaluation of the efficacy of Fuling Gancao Decoction in treating hyperlipidemia

[0057] Animal model of hyperlipidemia. Sixty SD rats were randomly divided into six groups: normal group, model group, low-, medium-, and high-dose compound treatment groups (9.9, 19.8, and 39.6 g / kg), and positive control / atorvastatin group (0.9 mg / kg). Ten rats were in each group. The normal group was fed a normal diet (29% primary wheat bran, 4.1% high-gluten flour, 40.84% ​​extruded corn, 2% fish meal, 16.06% soybean meal, 2% soybean oil, and 6% premix), while the other groups were fed a high-fat diet (protein, carbohydrate, and fat energy ratios of 24%, 41%, and 24%, respectively). The hyperlipidemia model was established after 12 weeks of feeding.

[0058] Except for the normal control group, the other five groups of rats were administered the drug by gavage once a day for 12 weeks. The normal control group rats were administered an equal volume of physiological saline by gavage once a day for 12 weeks.

[0059] Twelve weeks later, serum and organ tissues were collected from all rats after anesthesia. The specific gravity of liver, white and brown adipose tissue was measured. Serum and liver lipid indicators were detected. HE staining was used to observe pathological changes in liver and adipose tissue.

[0060] Serum and liver biochemical markers were measured. Following the kit instructions, TC, TG, HDL, and LDL levels were measured using an ELISA reader. Serum markers are as follows: Figure 1 As shown in the figure, compared with the control group and the model group, the low, medium, and high dose groups of Fuling Gancao Decoction all significantly reduced serum TG in hyperlipidemic rats (60%); the low dose group significantly reduced serum TC in hyperlipidemic rats (28%); and the medium dose group significantly reduced serum LDL-C in hyperlipidemic rats (37%). This indicates that Fuling Gancao Decoction can effectively improve blood lipids in the hyperlipidemic SD rat model and has a significant ameliorative effect on hyperlipidemia.

[0061] Liver indicators such as Figure 2 As shown in the figure, compared with the control group and the model group, the low-dose group of Fuling Gancao Decoction significantly reduced liver TC (26%). The low, medium, and high-dose groups all significantly reduced liver TC (33-40%). Simultaneously, Fuling Gancao Decoction also reduced blood glucose levels and liver coefficient in hyperlipidemic rats. Therefore, Fuling Gancao Decoction can regulate liver lipids and blood glucose in a hyperlipidemic rat model, and has a significant ameliorative effect on hyperlipidemia.

[0062] Morphological observation of liver and adipose tissue. Liver and adipose tissue were collected, fixed, dehydrated, embedded in paraffin, prepared into slides, stained with hematoxylin and eosin (HE), and observed under a light microscope. Results are as follows: Figure 3 As shown, the left image is liver tissue observed under a 100X microscope, and the right image is liver tissue observed under a 400X microscope. Green arrows represent hepatocyte steatosis, and blue arrows represent punctate necrosis of hepatocytes. Figure 3 The study found that Fuling Gancao Decoction can improve hepatocyte steatosis in a hyperlipidemic rat model.

[0063] like Figure 4 and Figure 5 As shown, Figure 4 The images show the morphological observation results of adipocytes in the white adipose tissue of SD rats. The left image shows the white adipose tissue observed under a 100X microscope, and the right image shows the white adipose tissue observed under a 400X microscope. Yellow arrows indicate increased adipocyte area. Figure 5 These are morphological observations of adipocytes in brown adipose tissue from SD rats. The left image shows brown adipose tissue observed under a 100X microscope, and the right image shows brown adipose tissue observed under a 400X microscope. Yellow arrows indicate increased adipocyte area. Figure 4 and Figure 5 As can be seen, compared with the normal group, the area of ​​white and brown adipose tissue cells in the model group was increased; the morphology of white and brown adipose tissue adipocytes in the high, medium and low dose groups was significantly improved compared with the model group.

[0064] like Figure 6 It can be seen that, compared with the control group and the model group, the low, medium and high dose groups of Fuling Gancao Decoction can significantly reduce the white adipose tissue index and increase the brown adipose tissue index and the proportion of brown adipose tissue (50%) in obese hyperlipidemic rats.

[0065] The above research results indicate that Fuling Gancao Decoction has the potential to regulate the browning of white fats.

[0066] Oil Red O staining of liver tissue. Liver tissue was collected, fixed, dehydrated, embedded in paraffin, prepared into slides, stained with Oil Red O, and observed under a light microscope. Results are as follows: Figure 7 As shown, after induction with a high-fat diet, the model group rats experienced severe lipid accumulation in their livers. Intervention with different doses of Fuling Gancao Decoction significantly improved the lipid accumulation in liver tissue to varying degrees.

[0067] Ultrastructural observation of mitochondria in adipose tissue. Samples were pre-fixed with 3% glutaraldehyde, re-fixed with 1% osmium tetroxide, dehydrated stepwise with acetone, embedded in Epon 812, and semi-thin sections were stained with toluidine blue for optical localization. Ultrathin sections were prepared using a diamond scalpel and observed under a JEM-1400FLASH transmission electron microscope after staining with uranium acetate and lead citrate.

[0068] The results are as follows Figure 8As shown, after induction with a high-fat diet, the white adipocytes of the model group rats exhibited significant morphological abnormalities. The nuclei were flattened and predominantly composed of euchromatin; there was severe loss of ribosomes in the cytoplasm; mitochondria were scarce and swollen, with cristae breakage and dissolution, matrix loss, and significant vacuolization. After intervention with Fuling Gancao Decoction, no significant improvement was observed in the low-dose group; in the medium-dose group, the white adipocytes showed slight morphological abnormalities, with a small loss of ribosomes in the cytoplasm, and most mitochondria exhibited relatively normal morphology and structure, appearing as a uniform gray color; in the high-dose group, the white adipocytes showed relatively normal morphology and structure, with a relatively abundant mitochondrial content in the cytoplasm, and the mitochondrial structures were intact, clear, and uniformly gray with distinct boundaries. The results indicate that Fuling Gancao Decoction can improve the morphological abnormalities of adipocytes and mitochondria induced by a high-fat diet.

[0069] See Figure 9 After induction with a high-fat diet, the brown adipocytes of rats in the model group showed significant morphological abnormalities. The nuclei were shrunken and irregularly shaped, with chromatin aggregation and slight widening of the perinuclear space in some areas. Mitochondria in the cytoplasm were swollen, enlarged, with cristae broken and dissolved, matrix lost, exhibiting vacuolation, and blurred mitochondrial boundaries. After treatment with Fuling Gancao Decoction, no significant improvement was observed in the low-dose group; in the medium-dose group, brown adipocytes showed mild morphological abnormalities; most mitochondria in the cytoplasm were swollen, with cristae broken and dissolved, matrix lost, exhibiting vacuolation, blurred mitochondrial boundaries, and a few mitochondria showing slight swelling; a small amount of autophagy was observed; in the high-dose group, brown adipocytes showed slight morphological abnormalities, most mitochondria in the cytoplasm were slightly swollen, with cristae broken and dissolved, matrix lost, electron density decreased, and mitochondrial boundaries were relatively blurred; a small amount of autophagy was observed. The results indicate that Fuling Gancao Decoction can improve brown adipocyte damage induced by a high-fat diet.

[0070] The embodiments described above merely illustrate specific implementation methods of this application, and while the descriptions are detailed and specific, they should not be construed as limiting the scope of protection of this application. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the technical solution of this application, and these modifications and improvements all fall within the scope of protection of this application.

Claims

1. The application of Fuling Gancao Decoction in the preparation of drugs for treating hyperlipidemia, characterized in that, The Poria and Licorice Decoction is made from the following ingredients in parts by weight: 2 parts Poria, 2 parts Cinnamon Twig, 1 part Prepared Licorice Root, and 3 parts Fresh Ginger.

2. The application of the Poria and Licorice Decoction according to claim 1 in the preparation of drugs for treating hyperlipidemia, characterized in that, The Poria and Licorice Decoction is a compound water extract.

3. The application of the Poria and Licorice Decoction according to claim 2 in the preparation of drugs for treating hyperlipidemia, characterized in that, The compound aqueous extract was prepared using the following method: Step S1: First decoction; Weigh each substance according to the weight parts of claim 1, add 8-10 times the weight of water, soak for 30-60 minutes, then heat to 100℃, maintain 100℃ for 1-1.5 hours, filter with gauze to obtain the first decoction residue and the first decoction aqueous solution. Step S2: Second decoction: Put the dregs from the first decoction into the pot, add 8-10 times the weight of water, heat to 100℃, keep at 100℃ for 30-60 minutes, filter to obtain the second decoction dregs and the second decoction liquid. Step S3: Mix the first decoction and the second decoction to obtain the compound water extract.

4. The application of the Poria and Licorice Decoction according to claim 1 in the preparation of drugs for treating hyperlipidemia, characterized in that, The Poria and Licorice Decoction is used to treat hyperlipidemia by lowering serum TG, TC and LDL-C.

5. The application of the Poria and Licorice Decoction according to claim 1 in the preparation of a drug for treating hyperlipidemia, characterized in that, The Poria and Licorice Decoction improves liver lipids and blood glucose in hyperlipidemic model animals and reduces liver coefficient to treat hyperlipidemia.

6. The application of the Poria and Licorice Decoction according to claim 1 in the preparation of a drug for treating hyperlipidemia, characterized in that, The Poria and Licorice Decoction improves hepatic steatosis and treats hyperlipidemia.

7. The application of the Poria and Licorice Decoction according to claim 1 in the preparation of a drug for treating hyperlipidemia, characterized in that, The Fuling Gancao Decoction was used to reverse the pathological morphology of adipose tissue cells and mitochondrial damage in a hyperlipidemia model animal to treat hyperlipidemia.

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