Determination of the contents of eight components in Bawei Chenxiang San

Abstract

This invention belongs to the field of traditional Chinese medicine detection technology, specifically relating to a method for determining the content of multiple components in Bawei Chenxiang San (Eight-Ingredient Agarwood Powder). The method employs gas chromatography to simultaneously determine the content of the main active components in Bawei Chenxiang San: octyl acetate, benzylacetone, myristole, and dehydrocostunolactone. This invention can effectively shorten the analysis time of the main active components in Bawei Chenxiang San, exhibits good linearity, high accuracy, precision, and repeatability, and is stable and feasible, applicable to product quality inspection.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine testing technology, specifically relating to a method for determining the content of multiple components in Eight-Ingredient Agarwood Powder. Background Technology

[0002] Eight-Ingredient Agarwood Powder, known as "Aga Jeba" in Tibetan medicine, is a prescription derived from the Four Medical Classics and is one of the few Tibetan medicine formulas included in the 2020 edition of the Pharmacopoeia of the People's Republic of China (hereinafter referred to as the "Chinese Pharmacopoeia"). This formula is a raw herbal powder made by grinding eight medicinal materials—agarwood, nutmeg, costus root, frankincense, jujube, chebula (baked), kapok flower, and travertine—into a fine powder, sifting, and mixing them evenly. It has the effects of clearing heat from the heart, nourishing the heart, calming the mind, and opening the orifices. It is a commonly used formula in Tibetan medicine clinical practice for treating febrile diseases affecting the heart, delirium, coronary heart disease, and angina pectoris. Modern pharmacological studies have confirmed that this product has therapeutic and protective effects against cardiovascular and cerebral hypoxia, ischemic-hypoxic heart disease, and myocardial ischemia-reperfusion injury caused by the low oxygen environment of high altitudes, and has significant clinical application value.

[0003] This formula contains four medicinal materials whose main active ingredients are volatile components: agarwood, nutmeg, costus root, and frankincense. Among them, benzylacetone in agarwood is a highly abundant and well-studied aromatic substance with antitussive, expectorant, and antiasthmatic effects. The volatile component of nutmeg is mainly myristyl ether, accounting for about 12.45% of the total volatile oil, which has effects such as protecting the heart, lowering blood sugar, and resisting ischemia-reperfusion myocardial injury. The volatile oil component of frankincense is octyl acetate, which has the largest content, accounting for 44.92% of the total volatile oil, and has pharmacological effects such as resisting hypoxia, protecting myocardial cells, improving myocardial hypertrophy, relieving pain, and alleviating coronary heart disease and angina pectoris. The volatile components of costus root are mainly sesquiterpenes such as costunolide and dehydrocostunolide, which have effects such as antioxidative damage, inhibiting oxidative stress, antispasmodic and analgesic, vasodilating, antioxidant, and cardiotonic effects. Therefore, Bawei Chenxiang San contains a variety of volatile components that have sedative, anti-hypoxic, vasodilatory, and cardioprotective effects. However, the quality standard for Bawei Chenxiang San in the Chinese Pharmacopoeia only specifies the thin-layer identification of costus root and the determination of costus root lactone content using HPLC. The quality control is not comprehensive enough, which affects the product's quality stability and clinical efficacy.

[0004] This invention establishes a method for determining the content of volatile index components such as octyl acetate, benzylacetone, myristyl ether, and dehydrocostunolide in the Eight-Flavor Agarwood Powder using GC method, providing a reference for the quality control of Tibetan medicine Eight-Flavor Agarwood and for improving and perfecting the quality standards included in the Chinese Pharmacopoeia. Summary of the Invention

[0005] The purpose of this invention is to provide a method for determining the content of multiple components in Eight-Flavor Agarwood Powder.

[0006] To achieve the above objectives, the technical solution of the present invention is as follows:

[0007] The Eight-Flavor Agarwood Powder of this invention is a medicinal powder composed of eight medicinal materials in a weight ratio of 2:1:1:1:1:1:1:1:1.

[0008] The method for determining the content of multiple components in the Eight-Ingredient Agarwood Powder of this invention employs gas chromatography to simultaneously determine the content of the main active ingredients, octyl acetate, benzylacetone, myristyl ether, and dehydrocostunolactone. The main active ingredients determined by this invention are all volatile components, primarily the active ingredients of agarwood, nutmeg, costus root, and frankincense. Among them, benzylacetone, a substance found in agarwood, is a highly abundant and well-studied aromatic compound with antitussive, expectorant, and antiasthmatic effects; myristole, the main volatile component in nutmeg, accounts for about 12.45% of the total volatile oil, and has effects such as protecting the heart, lowering blood sugar, and resisting ischemia-reperfusion myocardial injury; octyl acetate, the most abundant volatile oil component in frankincense, accounts for 44.92% of the total volatile oil, and has pharmacological effects such as resisting hypoxia, protecting myocardial cells, improving myocardial hypertrophy, relieving pain, and alleviating coronary heart disease and angina pectoris; and sesquiterpenoids such as costus lactone and dehydrocostus lactone, the main and characteristic volatile components of costus root, have effects such as antioxidative damage, inhibiting oxidative stress, relieving spasmolysis and pain, dilating blood vessels, antioxidation, and cardiotonic effects.

[0009] The chromatographic conditions of the gas chromatography method described in this invention are as follows: injection port temperature 220℃; flame ionization detector, detector temperature 240℃; capillary column, programmed column temperature rise; split injection, split ratio 10:1; carrier gas is nitrogen, make-up gas is nitrogen, and the flow rate ratio of carrier gas to make-up gas is 1:30; combustion gas is hydrogen, reactant gas is air, and the flow rate ratio of combustion gas to reactant gas is 1:10.

[0010] In the chromatographic conditions described in this invention, the injection port temperature is 220°C. Under this temperature condition, the methanol solutions of octyl acetate, benzyl acetone, myristole, and dehydrocostinol can be rapidly and uniformly vaporized, which is beneficial for the accurate analysis of the components.

[0011] The gas chromatography method described in this invention uses a capillary column (0.25 mm × 0.25 μm × 30 m). This column is a polar column with a bonded cross-linked polyethylene glycol stationary phase, with a film thickness of 0.25 μm, a column length of 30 m, and an inner diameter of 0.25 mm. This invention compared non-polar and polar capillary columns, and the results showed that using a non-polar column, dehydroauslide could not be separated from other component peaks, while using a polar capillary column effectively separated the main chromatographic peaks, resulting in more ideal chromatographic behavior.

[0012] The programmed column temperature rise of the capillary column under the chromatographic conditions described in this invention includes the following steps: a) raising the column temperature to an initial temperature of 40–60°C; b) raising the column temperature to 150°C at a rate of 40°C / min and holding for 2 min; c) raising the column temperature from 150°C to 175°C at a rate of 20°C / min and holding for 4 min; d) raising the column temperature from 175°C to 180°C at a rate of 5°C / min and holding for 2 min; e) raising the column temperature from 180°C to 200°C at a rate of 20°C / min and holding for 2 min; f) raising the column temperature from 200°C to 220°C at a rate of 10°C / min and holding for 2 min; g) raising the column temperature from 220°C to 230°C at a rate of 10°C / min and holding for 4 min. Steps a) and b) are for separating octyl acetate. Complete separation of octyl acetate from the solvent peak cannot be achieved at an initial temperature below 40℃ or above 60℃. Steps c) and d) are for separating benzylacetone. Steps e) and f) are for separating myristole. Step g) is for separating dehydroaurolactone.

[0013] The chromatographic conditions described in this invention employ split injection with a split ratio of 10:1, which means that the volume ratio of the vaporized sample entering the split outlet to the vaporized sample entering the capillary column is 10:1. Under this split ratio condition, solvent peak tailing can be effectively reduced, and the signal-to-noise ratio, sensitivity, and theoretical plate number of the chromatographic peak of the index component can be improved.

[0014] The method for preparing the test solution according to the present invention is as follows: add Bawei Chenxiang powder and chloroform to the extraction container at a weight-volume ratio of 1:20, weigh the mixture, use an ultrasonic device with a power of 250 W and a frequency of 40 kHz to extract the mixture for 10 minutes, weigh the mixture again, replenish the lost mass with chloroform, shake well, and filter the mixture through a 0.45 μm microporous membrane to obtain the test solution.

[0015] The eight-ingredient agarwood powder of this invention is a traditional raw herbal powder, made by directly grinding raw medicinal materials into powder and mixing them in a certain proportion. This invention studies, under the premise of determining the extraction solvent and extraction method, the effects of extraction time and solid-liquid ratio on four indicator components in the test sample solution through single-factor experiments. The results show that sonication for 10, 20, 30, and 40 min did not significantly change the content of indicator components in the test sample solution, but sonication for 60 min showed a decreasing trend in the content of indicator components. This may be because excessive sonication time leads to the dissolution of other non-indicator components, affecting the detection of the target components; therefore, sonication for 10 min was selected. Under the same conditions, the amount of extraction solvent was positively correlated with the content of the four indicator components in the test sample solution within a certain range. When the solid-liquid ratio was 1:20, 1:50, and 1:100, the changes in the content of indicator components in the test sample solution were not significant; therefore, a solid-liquid ratio of 1:20 was selected.

[0016] The gas chromatography method of this invention also includes a method for preparing reference solutions, which is as follows: First, accurately weigh the reference standards octyl acetate, benzyl acetone, myristole, and dehydrocostunolide, dissolve them separately in methanol, and shake well to prepare reference standard stock solutions of octyl acetate, benzyl acetone, myristole, and dehydrocostunolide. Then, mix the octyl acetate reference standard stock solution, benzyl acetone reference standard stock solution, myristole reference standard stock solution, and dehydrocostunolide reference standard stock solution in a volume ratio of 2:0.5:4:3, and dilute to volume with methanol to obtain a mixed reference standard solution of octyl acetate, benzyl acetone, myristole, and dehydrocostunolide.

[0017] The pure methanol solution described in this invention is methanol with a purity exceeding 99.9%.

[0018] The sample treatment method described in this invention involves ultrasonic extraction for 10 min, with eight-ingredient agarwood powder and chloroform at a weight-to-volume ratio of 1:20. The effects of steam distillation and ultrasonic extraction on four index components in the sample solution were compared. The inventors found that the volatile oil components extracted by steam distillation were too complex, severely interfering with the chromatographic peaks of the four index components and affecting their content detection. Therefore, this invention chooses to use ultrasonic extraction with organic solvents.

[0019] The gas chromatography method of the present invention also includes methods for preparing blank solutions and negative solutions: the blank solution method is to prepare a blank solution without adding the Eight-Flavor Agarwood Powder, and the remaining operations are the same as the preparation method of the test solution; the negative solution method is to prepare a negative solution lacking agarwood, nutmeg, costus root, and frankincense according to the prescription ratio of the Eight-Flavor Agarwood Powder, and the remaining operations are the same as the preparation method of the test solution.

[0020] The beneficial effects of this invention are:

[0021] (1) This invention establishes a gas chromatography method for the simultaneous quantitative determination of the index components octyl acetate, benzyl acetone, myristyl ether, and dehydrocostunolide in Bawei Chenxiang San and conducts methodological verification. The results show that the method is scientific and feasible.

[0022] (2) Compared with separate analysis, the present invention can effectively reduce the number of sample injections and analysis time of the instrument, with short detection time, accurate detection results, and simple and easy operation;

[0023] (3) The present invention has a wide linear range, good repeatability, stable and feasible sample processing method, and the obtained test solution has good stability at room temperature.

[0024] (4) The method of the present invention is stable and accurate, and can be used for the quality evaluation of Bawei Chenxiang Powder, and can provide a reference for improving the quality standard of Bawei Chenxiang Powder. Attached Figure Description

[0025] Figure 1 GC chromatogram of blank solution;

[0026] Figure 2 GC chromatogram of the mixed control solution;

[0027] Figure 3 The GC chromatogram of the test solution;

[0028] Figure 4 GC chromatogram of a negative solution;

[0029] Figure 5 The linear relationship graph for octyl acetate reference standard is shown.

[0030] Figure 6 The linear relationship graph for benzylacetone reference standard is shown.

[0031] Figure 7 Linearity graph for myristole reference standard;

[0032] Figure 8 The linear relationship graph is for dehydroauracetam reference standard. Detailed Implementation

[0033] The following experimental examples are used to further illustrate, but are not limited to, the present invention.

[0034] The eight-ingredient agarwood powder used in the following specific embodiments was purchased from Tibet Shenhou Pharmaceutical Co., Ltd., with batch numbers 20220601, 20220901, 20230301, and 20230501.

[0035] Octyl acetate reference standard (batch number: 111671-201704, purity 97.9%) was purchased from the National Institutes for Food and Drug Control; benzylacetone (batch number: G1528015, purity 98%) was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.; myristole reference standard (batch number: DST230415-054, purity 99.41%) and dehydrocostunolide reference standard (batch number: DSTDQ004202, purity 100.0%) were purchased from Chengdu Lemeitian Pharmaceutical Technology Co., Ltd.; chloroform (batch number: 20230914) was analytical grade, and methanol (batch number: 20230825) was chromatographic grade, purchased from Sinopharm Chemical Reagent Co., Ltd.

[0036] Travertine (batch number: 20200301) was purchased from Tibet Shenhou Pharmaceutical Co., Ltd.; Kapok (batch number: 20220601) and Terminalia chebula (baked) (batch number: 220701) were purchased from Anhui Yuankang Traditional Chinese Medicine Pieces Co., Ltd.; Jujube (batch number: 20230605) was purchased from Bozhou Miaoshantang Pharmaceutical Co., Ltd. Example

[0037] The contents of octyl acetate, benzyl acetone, myristole, and dehydrocostunolide in Bawei Chenxiang San were simultaneously determined by gas chromatography, including the following steps:

[0038] (1) Preparation of the test solution

[0039] Take 1 g of Eight-Flavor Agarwood Powder, accurately weigh it, place it in a 50 mL Erlenmeyer flask, accurately add 20 mL of chloroform, seal the flask, weigh it, sonicate it (power 250 W, frequency 40 kHz) for 10 min, cool it to room temperature, make up the weight with chloroform, shake well, filter it, and take the filtrate to filter through a 0.45 μm microporous membrane to obtain the test solution.

[0040] (2) Preparation of mixed reference solution

[0041] Accurately weigh 12.71 mg of octyl acetate reference standard, 11.84 mg of benzylacetone reference standard, 8.02 mg of myristole reference standard, and 10.41 mg of dehydroaurolactone reference standard. Place them in 10 mL volumetric flasks, dissolve and dilute to the mark with methanol, and shake well to prepare reference stock solutions with mass concentrations of 1244.31, 1160.32, 797.27, and 1041.00 μg / mL, respectively.

[0042] Accurately transfer 2 mL of octyl acetate reference stock solution, 0.5 mL of benzylacetone reference stock solution, 4 mL of myristole reference stock solution, and 3 mL of dehydroaurosinone reference stock solution into the same 20 mL volumetric flask, and dilute to the mark with methanol to prepare mixed reference solutions with mass concentrations of 124.43, 29.01, 159.45, and 156.15 μg / mL.

[0043] (3) Preparation of blank solution

[0044] Without adding the Eight-Flavor Agarwood Powder, the remaining operations are the same as the preparation method of the test sample solution under step (1) to obtain a blank solution.

[0045] (4) Preparation of negative solution

[0046] Based on the prescription ratio of the Eight-Flavor Agarwood Powder, the remaining operations are the same as the preparation method of the test sample solution under step (1) to prepare a negative solution lacking agarwood, nutmeg, costus root, and frankincense.

[0047] (5) Chromatographic conditions

[0048] An SH-WAX capillary column (0.25 mm × 0.25 μm × 30 m) was used; the injection port temperature was 220℃; the FID detector temperature was 240℃; the temperature program was as follows: initial temperature 40℃, increased to 150℃ at 40℃ / min, held for 2 min, increased to 175℃ at 20℃ / min, held for 4 min, increased to 180℃ at 5℃ / min, held for 2 min, increased to 200℃ at 20℃ / min, held for 2 min, increased to 220℃ at 10℃ / min, held for 2 min, increased to 230℃ at 10℃ / min, held for 4 min; split injection was used with a split ratio of 10:1 and an injection volume of 1 μL; the carrier gas was high-purity nitrogen at a flow rate of 1 mL / min, the air flow rate was 400 mL / min, the hydrogen flow rate was 40 mL / min, and the nitrogen tail gas flow rate was 30 mL / min.

[0049] (6) Methodological investigation

[0050] System suitability test: Blank solution, mixed reference solution, test solution, and negative solution were injected and analyzed according to chromatographic conditions. Chromatograms were recorded, and results are shown in [see table below]. Figure 1 , Figure 2 , Figure 3 , Figure 4 The chromatograms obtained from the blank and negative solutions showed no interfering peaks at the retention times of octyl acetate, benzylacetone, myristole, and dehydrocostunolide. In the chromatogram of the test solution, the resolution of octyl acetate, benzylacetone, myristole, and dehydrocostunolide from adjacent peaks was greater than 1.5, the tailing factor was between 0.95 and 1.05, the theoretical plate number was greater than 10,000, and the retention times were consistent with those of the components in the reference solution. The suitability test of the chromatographic system met the requirements for content determination.

[0051] Linearity investigation: Accurately pipette 0.5, 1, 1.5, 2, 2.5, 3, and 5 mL of the mixed reference solution into 5 mL volumetric flasks, dilute to volume with methanol, and mix well to prepare mixed reference solutions of different concentrations. Inject and determine the concentrations according to chromatographic conditions. Plot the mass concentration of the reference solution as the x-axis (…). X The peak area is the vertical axis ( Y Linear regression analysis showed that octyl acetate exhibited a good linear relationship with the peak area integral value within the range of 12.44–124.43 μg / mL, as indicated by the equation: Y = 505.36 X +3132.2 ( r = 0.999 5), benzylacetone showed a good linear relationship with the peak area integral value in the range of 2.90~29.01 μg / mL, and the equation was: Y = 759.01 X+ 534.22 ( r = 0.999 6), myristole showed a good linear relationship with the peak area integral value in the range of 15.95~159.45 μg / mL, and the equation was: Y = 597.78 X +762.35 ( r = 0.999 8), dehydrocostrolactone showed a good linear relationship with the peak area integral value in the range of 15.62~156.15 μg / mL, and the equation was: Y = 738.62 X +485.43 ( r = 0.999 6), the result is as follows Figure 5 , Figure 6 , Figure 7 , Figure 8 .

[0052] Precision test: Take the test solution, inject it according to the chromatographic conditions, and measure it 6 times consecutively. The RSD (n=6) of the peak areas of octyl acetate, benzylacetone, myristole, and dehydrocostinol were 0.94%, 1.06%, 0.63%, and 1.79%, respectively, indicating that the instrument has good precision.

[0053] Repeatability test: Six test solutions were prepared in parallel using the same batch of eight-ingredient agarwood powder (batch number 20220601) according to the test solution preparation method. The solutions were injected and determined under chromatographic conditions. The average contents of octyl acetate, benzylacetone, myristole, and dehydrocostunolide were 0.917 mg / g, 0.104 mg / g, 1.210 mg / g, and 1.234 mg / g, respectively, and the RSDs (n=6) were 0.99%, 0.74%, 0.91%, and 0.84%, respectively, indicating that the repeatability of this method is good.

[0054] Stability test: A fresh test solution was prepared and injected at room temperature at 0, 2, 4, 6, 8, 10, 12, and 24 h according to chromatographic conditions. The RSD (n=8) values ​​of the peak areas of octyl acetate, benzylacetone, myristole, and dehydrocostunolide were 1.40%, 0.59%, 1.18%, and 1.24%, respectively. This indicates that the test solution is stable within 24 h.

[0055] Recovery test by spiking: Six samples of the known content of the eight-ingredient agarwood powder (batch number 20220601), each approximately 1 g, were accurately weighed. Reference standards were added to prepare six test solutions, which were then injected and determined according to chromatographic conditions. The average recovery rate and RSD were calculated. The average recoveries (n=6) of octyl acetate, benzylacetone, myristole, and dehydrocostunolide were 100.40% (RSD=1.55%), 97.80% (RSD=1.41%), 99.50% (RSD=0.77%), and 99.50% (RSD=0.85%), respectively.

[0056] (7) Determination of the content of multiple components of Bawei Chenxiang Powder

[0057] Three batches of Eight-Flavor Agarwood Powder (batch numbers: 20220901, 20230301, and 20230501) were collected. Two samples were taken from each batch, and test solutions were prepared separately. The samples were injected and determined according to chromatographic conditions. The results are shown in Table 1. Table 1: Determination of the content of four components in Eight-Flavor Agarwood Powder (n=2)

[0058] batch number <![CDATA[Octyl acetate / mg·g -1 > <![CDATA[Benzylacetone / mg·g -1 > <![CDATA[Myristicin / mg·g -1 > <![CDATA[Dehydrocostus lactone / mg·g -1 > 20220901 0.91 0.10 1.20 1.21 20230301 0.92 0.10 1.22 1.24 20230501 0.91 0.10 1.20 1.24

[0059] This invention establishes a gas chromatography method for the simultaneous quantitative determination of key components in Bawei Chenxiang San (Eight-Ingredient Agarwood Powder), including octyl acetate, benzylacetone, myristole, and dehydrocostunolactone. The method has been validated, demonstrating its scientific validity and feasibility. Compared to individual analyses, this gas chromatography-programmed temperature method effectively reduces the number of instrument injections and analysis time, shortening the experimental time and lowering costs. Furthermore, this invention exhibits a wide linear range, good repeatability, and a stable and feasible sample preparation method. The resulting test solution demonstrates good stability at room temperature. This method is stable and accurate, suitable for the quality evaluation of Bawei Chenxiang San, and can provide a reference for improving the quality standards of this product.

[0060] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, and is not intended to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions made by those skilled in the art to the technical solution of the present invention do not depart from the essence and scope of the technical solution of the present invention.

Claims

1. A method for determining the content of multiple components in Eight-Flavor Agarwood Powder, characterized in that, The contents of octyl acetate, benzyl acetone, myristole, and dehydrocostunolide in Bawei Chenxiang San were simultaneously determined by gas chromatography. The chromatographic conditions of the gas chromatography method were: injection port temperature 220℃; flame ionization detector, detector temperature 240℃; capillary column, programmed column temperature. Split injection with a split ratio of 10:1; nitrogen as both carrier and make-up gas; hydrogen as combustion gas and air as reactant gas; the programmed column temperature rise for the capillary column under the chromatographic conditions includes the following steps: a) The column temperature rises to the initial temperature of 40–60°C; b) Increase the column temperature to 150℃ at a rate of 40℃ / min and hold for 2 min; c) Increase the column temperature from 150℃ to 175℃ at a rate of 20℃ / min, and hold for 4 min; d) Increase the column temperature from 175℃ to 180℃ at a rate of 5℃ / min, and hold for 2 min; e) Increase the column temperature from 180°C to 200°C at a rate of 20°C / min, and hold for 2 min; f) Increase the column temperature from 200°C to 220°C at a rate of 10°C / min, and hold for 2 min. g) Increase the column temperature from 220℃ to 230℃ at a rate of 10℃ / min, and hold for 4 min; The flow rates of the carrier gas, combustion gas, reaction gas, and tail gas are 1 mL / min, 40 mL / min, 400 mL / min, and 30 mL / min, respectively. The gas chromatographic capillary column is a polar chromatographic column with a bonded cross-linked polyethylene glycol stationary phase, with a film thickness of 0.25 μm, a column length of 30 m, and an inner diameter of 0.25 mm. The test solution is prepared as follows: Add the eight-flavor agarwood powder and chloroform to the extraction container at a weight-volume ratio of 1:20, weigh the mixture, sonicate for 10 minutes, weigh the mixture again, replenish the lost mass with chloroform, shake well, and filter through a 0.45μm microporous membrane to obtain the test solution. The preparation method of the reference solution is as follows: accurately weigh the reference standards octyl acetate, benzylacetone, myristole, and dehydrocostunolide, dissolve them separately in methanol, shake well, and prepare the stock solutions of each reference standard; take the stock solutions of octyl acetate, benzylacetone, myristole, and dehydrocostunolide in a volume ratio of 2:0.5:4:3, place them in the same volumetric flask, add methanol to make up to volume, and prepare a mixed reference solution.

2. The method for determining the content of multiple components in the eight-ingredient agarwood powder according to claim 1, characterized in that, The injection volume in the gas chromatography method is 1 μL.

3. The method for determining the content of multiple components in the eight-ingredient agarwood powder according to claim 1, characterized in that, The Eight-Flavor Agarwood Powder is a pharmaceutical composition consisting of eight medicinal materials—agarwood, nutmeg, costus root, frankincense, jujube, chebula (baked), kapok flower, and travertine—in a weight ratio of 2:1:1:1:1:1:1:1, or a preparation made from the pharmaceutical composition and a pharmaceutical carrier.

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