Application of NtPPO7 gene in regulating chlorogenic acid content in tobacco leaves and tobacco variety breeding
By cloning and overexpressing the tobacco polyphenol oxidase gene NtPPO7, the problem of regulating chlorogenic acid content in tobacco was solved, resulting in improved tobacco quality and stress resistance, and providing new gene resources for tobacco variety breeding.
CN118652913BActive Publication Date: 2026-07-21ZHENGZHOU TOBACCO RES INST OF CNTC
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Patent Information
- Application Number
- CN202410821394.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-24
- Publication Date
- 2026-07-21
- Estimated Expiration
- 2044-06-24
AI Technical Summary
Technical Problem
Current technologies cannot effectively regulate the chlorogenic acid content in tobacco, which affects tobacco quality and stress resistance.
Method used
By cloning and overexpressing the tobacco polyphenol oxidase gene NtPPO7, constructing an expression vector, and using genetic engineering technology to overexpress the NtPPO7 gene in tobacco, the content of chlorogenic acid in mature leaves was significantly reduced.
Benefits of technology
It significantly reduces the content of chlorogenic acid in tobacco leaves, regulates tobacco aroma and stress resistance, and provides new genetic resources for tobacco variety breeding.
✦ Generated by Eureka AI based on patent content.
Abstract
The application discloses application of NtPPO7 gene in regulating chlorogenic acid content in tobacco leaves and tobacco variety cultivation, and belongs to the technical field of plant genetic engineering. The cDNA of leaves of common tobacco at different periods is used as a template, and a homologous gene of polyphenol oxidase of tobacco is cloned by using PCR technology, and is named as NtPPO7. It is found through cloning of the NtPPO7 gene and construction and analysis of an expression vector that overexpression of the gene in tobacco plants can significantly reduce the content of chlorogenic acid in mature tobacco leaves, which indicates that the NtPPO7 gene has an important role in regulation of chlorogenic acid in mature tobacco leaves. The application enriches the polyphenol oxidase gene family of tobacco, and lays a foundation for in-depth study of the polyphenol oxidase gene family in tobacco. Meanwhile, the application lays a foundation for elucidating a molecular regulation mechanism of chlorogenic acid biosynthesis in tobacco leaves, and provides a new precise regulation gene for regulation of the content of chlorogenic acid in tobacco leaves.
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