Molecular marker and method for identifying drought resistance of wheat

Abstract

The application discloses a molecular marker and a method for identifying drought resistance of wheat. The molecular marker disclosed by the application is a nucleotide corresponding to the 642-643th nucleotide of SEQ ID No. 1 in a sequence listing in a wheat genome, which is GC or CT. Experiments prove that the drought resistance of a homozygous wheat with the nucleotide corresponding to the 642-643th nucleotide of SEQ ID No. 1 in a sequence listing in a genome being GC is greater than that of a homozygous wheat with the nucleotide corresponding to the 642-643th nucleotide of SEQ ID No. 1 in a sequence listing being CT. The molecular marker of the application is related to the drought resistance of wheat, and the drought-resistant wheat can be quickly and accurately found by detecting the wheat molecular marker of the application. The application has important significance in cultivating stress-resistant wheat varieties or research.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of biotechnology, and in particular to a molecular marker and method for identifying drought resistance of wheat. BACKGROUND

[0002] Drought is one of the main environmental disasters affecting crop production and causing food reduction. Wheat (Triticum aestivum L.) is one of the three major crops in China, and its production is crucial to the national food security. Therefore, ensuring the growth and yield of wheat under drought conditions is of great strategic significance to maintaining the food security of China. With the development of molecular biology, molecular marker-assisted breeding provides a convenient and efficient method for selecting target traits of wheat. Utilizing molecular markers to explore and utilize excellent genetic resources can improve the efficiency of breeding and provide an efficient way for crop genetic improvement and germplasm innovation. SUMMARY

[0003] The present application solves the technical problem of how to detect the drought resistance of wheat.

[0004] To solve the above technical problems, the present application first provides an application of a substance for detecting a molecular marker of wheat in detecting or assisting in detecting the drought resistance of wheat.

[0005] The molecular marker of wheat is a nucleotide corresponding to position 642 and / or position 643 of SEQ ID No. 1 in the sequence listing, and the nucleotide corresponding to position 642 of SEQ ID No. 1 in the sequence listing is G or C, and the nucleotide corresponding to position 643 of SEQ ID No. 1 in the sequence listing is C or T.

[0006] In the above application, the substance for detecting the molecular marker of wheat can be a primer pair named TaGATA-2B-Primer or a complete set of reagents for detecting the molecular marker of wheat.

[0007] The TaGATA-2B-Primer is composed of two single-stranded DNAs shown in SEQ ID No. 4 and SEQ ID No. 5 in the sequence listing.

[0008] The complete set of reagents for detecting the molecular marker of wheat includes a primer pair named TaGATA-2B-Primer-APaLI, and the TaGATA-2B-Primer-APaLI is composed of two single-stranded DNAs shown in SEQ ID No. 2 and SEQ ID No. 3 in the sequence listing.

[0009] In the above application, the complete set of reagents can further include a restriction enzyme APaLI.

[0010] The kit can be the TaGATA-2B-Primer-APaLI, and can also consist of the TaGATA-2B-Primer-APaLI and the restriction enzyme APaLI.

[0011] In the above application, the drought resistance of the homozygous wheat in which the nucleotide corresponding to position 642 of SEQ ID No. 1 in the sequence listing in the genome is G is greater than or is candidate to be greater than the drought resistance of the homozygous wheat in which the nucleotide corresponding to position 642 of SEQ ID No. 1 in the sequence listing in the genome is C;

[0012] The drought resistance of the homozygous wheat in which the nucleotide corresponding to position 643 of SEQ ID No. 1 in the sequence listing in the genome is C is greater than or is candidate to be greater than the drought resistance of the homozygous wheat in which the nucleotide corresponding to position 643 of SEQ ID No. 1 in the sequence listing in the genome is T;

[0013] The drought resistance of the homozygous wheat in which the nucleotides corresponding to positions 642 and 643 of SEQ ID No. 1 in the sequence listing in the genome are G and C in turn is greater than or is candidate to be greater than the drought resistance of the homozygous wheat in which the nucleotides corresponding to positions 642 and 643 of SEQ ID No. 1 in the sequence listing in the genome are C and T in turn.

[0014] The application also provides a method for detecting or assisting in detecting the drought resistance of wheat, which comprises detecting the molecular marker of the wheat, and determining the drought resistance of the wheat according to the following method:

[0015] The drought resistance of the homozygous wheat in which the nucleotide corresponding to position 642 of SEQ ID No. 1 in the sequence listing in the genome is G is greater than or is candidate to be greater than the drought resistance of the homozygous wheat in which the nucleotide corresponding to position 642 of SEQ ID No. 1 in the sequence listing in the genome is C;

[0016] The drought resistance of the homozygous wheat in which the nucleotide corresponding to position 643 of SEQ ID No. 1 in the sequence listing in the genome is C is greater than or is candidate to be greater than the drought resistance of the homozygous wheat in which the nucleotide corresponding to position 643 of SEQ ID No. 1 in the sequence listing in the genome is T;

[0017] The drought resistance of the homozygous wheat in which the nucleotides corresponding to positions 642 and 643 of SEQ ID No. 1 in the sequence listing in the genome are G and C in turn is greater than or is candidate to be greater than the drought resistance of the homozygous wheat in which the nucleotides corresponding to positions 642 and 643 of SEQ ID No. 1 in the sequence listing in the genome are C and T in turn.

[0018] In the above method, the detection of the molecular marker of the wheat can be performed by using the substance for detecting the molecular marker of the wheat.

[0019] In the method, the detection of the wheat molecular marker by the TaGATA-2B-Primer can include: performing PCR amplification on the wheat genomic DNA by the TaGATA-2B-Primer to obtain an amplification product; and sequencing the amplification product to determine the nucleotide corresponding to the 642nd and / or 643rd nucleotide of SEQ ID No. 1 in the sequence listing in the wheat genome.

[0020] The reaction system of the PCR amplification can be ddH2O 12.2 μL, 5×PCR buffer 4.0 μL, TaGATA-2B-Primer-F (10 μmol / L) and TaGATA-2B-Primer-R (10 μmol / L) each 0.4 μL, dNTPs (2.5 mmol / L) 1.6 μL, transfast pfu enzyme (5 U) 0.4 μL, template DNA (20 ng / μL) 1 μL. Both 5×PCR buffer and transfast pfu enzyme (5 U) are products of Beijing Quanshi Gold Biotechnology Co., Ltd.

[0021] The reaction condition of the PCR amplification can be 95°C for 5 min; 95°C for 30 s, 60°C for 30 s, 72°C for 45 s, 35 cycles; 72°C for 10 min, 4°C storage.

[0022] In the method, the detection of the wheat molecular marker by the kit can include: performing PCR amplification on the wheat genomic DNA by the TaGATA-2B-Primer-APaLI to obtain an amplification product; performing enzyme digestion on the amplification product by APaLI to obtain an enzyme digestion product, detecting the size of the enzyme digestion product, and determining the nucleotide corresponding to the 642nd and / or 643rd nucleotide of SEQ ID No. 1 in the sequence listing in the wheat genome according to the linkage between the G at the 642nd position and the C at the 643rd position and the linkage between the C at the 642nd position and the T at the 643rd position according to the following method:

[0023] If the enzyme digestion product contains a DNA fragment with a size of 185 bp and does not contain DNA fragments with sizes of 167 bp and 18 bp, respectively, then the nucleotide corresponding to the 642nd nucleotide of SEQ ID No. 1 in the sequence listing in the wheat genomic DNA is G, and the nucleotide corresponding to the 643rd nucleotide of SEQ ID No. 1 in the sequence listing is C;

[0024] If the enzyme digestion product contains two DNA fragments with sizes of 167 bp and 18 bp respectively and does not contain a DNA fragment with a size of 185 bp, then the nucleotide corresponding to position 642 of SEQ ID No. 1 in the sequence listing in the wheat genomic DNA is C, and the nucleotide corresponding to position 643 of SEQ ID No. 1 in the sequence listing is T;

[0025] If the enzyme digestion product contains three DNA fragments with sizes of 185 bp, 167 bp and 18 bp respectively, then the nucleotide corresponding to position 642 of SEQ ID No. 1 in the sequence listing in the wheat genomic DNA is G and C (i.e. G at this position on one chromosome and C at this position on another chromosome), and the nucleotide corresponding to position 643 of SEQ ID No. 1 in the sequence listing is C and T (i.e. C at this position on one chromosome and T at this position on another chromosome).

[0026] The substance for detecting the wheat molecular marker also falls within the protection scope of the present application.

[0027] The wheat molecular marker also falls within the protection scope of the present application.

[0028] The present application also provides any of the following applications:

[0029] X1) the wheat molecular marker in wheat breeding;

[0030] X2) the wheat molecular marker in detecting or assisting in detecting drought resistance of wheat;

[0031] X3) the substance for detecting the wheat molecular marker in wheat breeding;

[0032] X4) the substance for detecting the wheat molecular marker in preparing a wheat breeding product;

[0033] X5) the substance for detecting the wheat molecular marker in preparing a product for detecting or assisting in detecting drought resistance of wheat;

[0034] X6) the method for detecting or assisting in detecting drought resistance of wheat in wheat breeding;

[0035] X7) the substance for detecting the nucleotides corresponding to positions 642 and 643 of SEQ ID No. 1 in the sequence listing in the wheat genome in breeding drought-resistant wheat;

[0036] X8) the substance for detecting the nucleotides corresponding to positions 642 and 643 of SEQ ID No. 1 in the sequence listing in the wheat genome in preparing a product for breeding drought-resistant wheat.

[0037] The application also provides a wheat breeding method, which comprises I, II or III:

[0038] I, detecting the nucleotide corresponding to the 642th and 643th nucleotide of SEQ ID No. 1 in the sequence listing in the wheat genome, and selecting the wheat with G and C at the 642th and 643th nucleotide of SEQ ID No. 1 in the sequence listing in the wheat genome as the parent for breeding;

[0039] II, detecting the nucleotide corresponding to the 642th nucleotide of SEQ ID No. 1 in the sequence listing in the wheat genome, and selecting the wheat with G at the 642th nucleotide of SEQ ID No. 1 in the sequence listing in the wheat genome as the parent for breeding;

[0040] III, detecting the nucleotide corresponding to the 643th nucleotide of SEQ ID No. 1 in the sequence listing in the wheat genome, and selecting the wheat with C at the 643th nucleotide of SEQ ID No. 1 in the sequence listing in the wheat genome as the parent for breeding.

[0041] The application detects the drought resistance of wheat by comparing the thousand-grain weight of wheat under sufficient water and insufficient water, and the drought resistance is reflected in the drought resistance coefficient of the thousand-grain weight, which is the ratio of the thousand-grain weight of wheat under insufficient water (rain-fed) to the thousand-grain weight of wheat under sufficient water (irrigation).

[0042] In the application, the wheat can be any one of 32 wheat materials in Table 1 or its offspring, and the 32 wheat materials are PANDAS, An85zhong124-1, Yanzhan No.1, Bawangbian, Beijing No.10, Beijing No.14, Cangzhou Wheat, Changwu131, Chang6878, Dali No.1, DanR8093, Fengkang13, Jimai41, Jimai No.6, Jin2148-7, Jinghe8922, LinKang5108, Baiqimai, Changle No.5, Hongheshang, Beijing8686, 04-044, 04-030, Chun22 9th-25, Zilangbaimangxian, Jingpin No.10, Chun04 9th-5-1, Chun45 9th-50-1, Neixiang188, Jing411, Zhongguochun and Baishuomai.

[0043] Experiments prove that the drought resistance of wheat with GC at the 642th-643th nucleotide of SEQ ID No. 1 in the sequence listing in the genome is greater than that of wheat with CT at the site. The wheat molecular marker of the application can be used to quickly and accurately find drought-resistant wheat. The application provides a new method for wheat molecular marker assisted selection breeding, which has important significance in cultivating drought-resistant wheat varieties or research.

[0044] The present application will be further described in conjunction with the specific embodiments. The examples given are only to illustrate the present application, and are not intended to limit the scope of the present application. The examples provided below can serve as a guide for further improvement by those of ordinary skill in the art, and do not in any way constitute a limitation on the present application. BRIEF DESCRIPTION OF DRAWINGS

[0045] Figure 1 Part of the molecular marker detection results of the present application. In which, the electrophoresis band size of the GC lane is 185bp, the electrophoresis band size of the CT lane is 167bp and 18bp respectively, and the small fragment band of 18bp is relatively blurred due to the long electrophoresis time. The M lane is a DNA molecular weight standard.

[0046] Figure 2 Statistical results of the thousand-grain weight drought resistance coefficient of different homozygous types of TaGATA-2B gene in a natural population of wheat. DETAILED DESCRIPTION

[0047] In the following examples, the experimental methods are all conventional methods, and are performed according to the techniques or conditions described in the literature in the art or according to the product instructions, unless otherwise specified. In the following examples, the materials, reagents, instruments, etc. used, unless otherwise specified, can be obtained from commercial channels. In the quantitative tests in the following examples, at least three repeated experiments were set up, and the results were averaged. In the following examples, unless otherwise specified, the 1st position of each nucleotide sequence in the sequence listing is the 5' terminal nucleotide of the corresponding DNA / RNA, and the last position is the 3' terminal nucleotide of the corresponding DNA / RNA.

[0048] Example 1: Obtaining of a drought resistance related molecular marker of wheat

[0049] I. Obtaining of TaGATA-2B gene polymorphic site

[0050] 1. Design specific primers for the genome of TaGATA-2B gene according to the characteristics of the genomic DNA sequence of wheat TaGATA-2B gene, and the primer sequences are as follows:

[0051] TaGATA-2B-Primer-F (forward primer): 5'-ACTGCTTGCATTTGTTGTGC-3' (SEQ ID No. 4 in the sequence listing);

[0052] TaGATA-2B-Primer-R (reverse primer): 5'-GGCGGGAGGATGTTGTACTC-3' (SEQ ID No. 5 in the sequence listing).

[0053] The recognition sequences of TaGATA-2B-Primer-F and TaGATA-2B-Primer-R are located at the upstream of TaGATA-2B gene and the third exon, respectively.

[0054] 2. Using the genomic DNA of 32 wheat materials (all from the National Genebank) as templates, the primers in step 1 were used for PCR amplification to obtain PCR amplification products, and then the PCR amplification products were sequenced and compared.

[0055] The PCR amplification system (20 μL) used was as follows: ddH2O 12.2 μL, 5×PCR buffer 4.0 μL, forward primer (10 μmol / L) and reverse primer (10 μmol / L) 0.4 μL each, dNTPs (2.5 mmol / L) 1.6 μL, transfast pfu enzyme (5 U) 0.4 μL, template DNA (20 ng / μL) 1 μL. 5×PCR buffer and transfast pfu enzyme (5 U) were both products of Beijing ZhenGsi Jin Biotechnology Co., Ltd.

[0056] The PCR amplification conditions were as follows: 95 °C for 5 min; 95 °C for 30 s, 60 °C for 30 s, 72 °C for 45 s, 35 cycles; 72 °C for 10 min, 4 °C for storage.

[0057] Through sequence analysis, it was found that there were two SNP sites in the TaGATA-2B genomic DNA (the sequence was SEQ ID No. 1), which were the 642-643 sites of TaGATA-2B gene (there were GC and CT polymorphisms, S represented G or C, and Y represented C or T), and were marked as molecular marker TaGATA-2B-642-643. In the 32 wheat materials, it was also found that the G at the 642 site and the C at the 643 site of SEQ ID No. 1 were linked, and the C at the 642 site and the T at the 643 site were linked.

[0058] The names of the 32 wheat materials used were as follows: PANDAS, An 85zhong 124-1, Yanzhan 1, Bawangbian, Beijing 10, Beijing 14, Cangzhou wheat, Changwu 131, Chang 6878, Dali 1, Dan R8093, Fengkang 13, Jimai 41, Jimai 6, Jin 2148-7, Jinghe 8922, Lin Kang 5108, Baiqimai, Changle 5, Hongheshang, Beijing 8686, 04-044, 04-030, Chun 229th-25, Ziganbaomangxian, Jingpin 10, Chun 04 9th-5-1, Chun 45 9th-50-1, Neixiang 188, Jing 411, Zhongguochun, Baishuomai.

[0059] II. Detection of the molecular marker

[0060] 1. For the molecular marker at positions 642-643 of the TaGATA-2B gene shown in SEQ ID No. 1 in the sequence listing, a primer capable of detecting the marker is designed, and the primer sequence is as follows:

[0061] TaGATA-2B-Primer-APaLI-F (forward primer): 5'- AGTTGAACGACGGGCCCGTGCA-3' (SEQ ID No. 2);

[0062] TaGATA-2B-Primer-APaLI-R (reverse primer): 5'- AAATACACAGCAAGGCAGCA-3' (SEQ ID No. 3).

[0063]

[0064] 2. The steps for detecting the molecular marker of the wheat to be tested using the primer of step 1 are as follows:

[0065] The genomic DNA of the wheat to be tested is used as a template, and the primer of step 1 is used for PCR amplification to obtain PCR amplification product A. The nucleotide sequence of the PCR amplification product A is positions 620 to 804 of SEQ ID No. 1.

[0066] The system (10 μL) for PCR amplification is as follows: ddH2O 3.6 μL, 0.2 μL of the forward primer (10 μmol / L) and the reverse primer (10 μmol / L) respectively, 2 × PCR Mix 5 μL, template DNA (20 ng / μL) 1 μL. Among them, 2 × PCR Mix is a product of Zhuangmeng Biological Company, and the article number is ZT201A.

[0067] The PCR amplification conditions are as follows: 95 ℃ for 5 min; 95 ℃ for 30 s, 60 ℃ for 30 s, 72 ℃ for 20 s, 35 cycles; 72 ℃ for 10 min, 4 ℃ for storage.

[0068] The PCR amplification product A obtained in step 1 is digested with restriction endonuclease APaLI to obtain enzyme digestion product B, and the electrophoretic detection result of part of the enzyme digestion product B is as shown in Figure 1 ​If the enzyme digestion product B contains a DNA fragment with a size of 185 bp and does not contain DNA fragments with sizes of 167 bp and 18 bp, respectively, it indicates that the nucleotide corresponding to positions 642-643 of SEQ ID No. 1 in the sequence listing in the genome DNA of the wheat to be tested is GC, and the wheat to be tested is a homozygous wheat; if the enzyme digestion product B contains two DNA fragments with sizes of 167 bp and 18 bp, respectively, and does not contain a DNA fragment with a size of 185 bp, it indicates that the nucleotide corresponding to positions 642-643 of SEQ ID No. 1 in the sequence listing in the genome DNA of the wheat to be tested is CT, and the wheat to be tested is a homozygous wheat; if the enzyme digestion product B contains three DNA fragments with sizes of 185 bp, 167 bp and 18 bp, respectively, it indicates that the nucleotide corresponding to positions 642-643 of SEQ ID No. 1 in the sequence listing in the genome DNA of the wheat to be tested is GC and CT, and the wheat to be tested is a heterozygous wheat.

[0069] Example 2, Analysis of the correlation between the wheat molecular marker and drought resistance

[0070] The natural population (Table 1) was genotyped by using the molecular marker in Example 1, and the correlation between the molecular marker and drought resistance was analyzed. The specific steps are as follows:

[0071] 1. Detection of the molecular marker

[0072] Each wheat in the natural population consisting of 323 hexaploid wheat was used as the wheat to be tested, and the detection of the wheat molecular marker was performed according to the method in step two of Example 1, so as to determine the nucleotide corresponding to positions 642-643 of SEQ ID No. 1 in the sequence listing of each wheat individual. Each wheat variety in the natural population was from the National Germplasm Bank.

[0073] The detection results of the molecular markers are shown in Table 1, "GC" represents homozygous type of the nucleotides corresponding to positions 642-643 of SEQ ID No. 1 in the sequence listing being GC, "CT" represents homozygous type of the nucleotides corresponding to positions 642-643 of SEQ ID No. 1 in the sequence listing being CT, and "-" represents heterozygous type of the nucleotides corresponding to positions 642-643 of SEQ ID No. 1 in the sequence listing being GC and CT. Then, the genomic DNA of each wheat was amplified and sequenced using the primer pair consisting of TaGATA-2B-Primer-F and TaGATA-2B-Primer-R of Example 1, and the amplification system and conditions were the same as those of Example 1. The results showed that the sequence of the PCR product of each wheat was SEQ ID No. 1 except for the two SNP sites (positions 642 and 643 of SEQ ID No. 1), and the two SNP sites both met the linkage of G at position 642 and C at position 643 of SEQ ID No. 1, and the linkage of C at position 642 and T at position 643 of SEQ ID No. 1.

[0074] Table 1, detection results of each wheat molecular marker in natural population

[0075]

[0076]

[0077]

[0078]

[0079]

[0080] 2, correlation analysis of molecular markers and drought resistance

[0081] The natural population of wheat was planted in Changping (116°13' east longitude; 40°13' east latitude) for two growth seasons (2015-2016 and 2016-2017), and two water conditions treatments were set, i.e. rain-fed (rain-fed during the whole growth period, and the total precipitation in the two growth seasons was 173 and 143 mm, respectively) and irrigation (water sufficient, irrigation was carried out before overwintering, at flowering stage and at grain filling stage, and the irrigation amount was 750 mm, i.e. 75 mm). 3 hm -1 That is, 75 mm). Each test area was 2 meters long, and there were 4 rows with a row spacing of 30 cm. Each row contained 40 seeds. After the wheat was harvested, the thousand-grain weight of different wheat varieties was measured, and the average thousand-grain weight drought resistance coefficient of each year was calculated.

[0082] Thousand-grain weight drought resistance coefficient = rain-fed thousand-grain weight / irrigation thousand-grain weight.

[0083] The molecular marker (i.e. the nucleotide at position 642-643 of SEQ ID No. 1) in Example 1 was used to analyze the correlation between the molecular marker and the drought resistance coefficient of 1000-grain weight by using GLM model in Tassel 5.0 software.

[0084] The statistical results of the drought resistance coefficient of 1000-grain weight of each wheat are shown in Table 2. The drought resistance coefficient of 1000-grain weight of the wheat with the homozygous type of GC at position 642-643 of SEQ ID No. 1 in the sequence listing was significantly greater than that of the wheat with the homozygous type of CT at the position. It is indicated that the molecular marker of the present application is related to the drought resistance of wheat, and can be used for breeding drought-resistant wheat varieties.

[0085] Table 2, the drought resistance coefficient of 1000-grain weight of different homozygous types in a natural population of wheat

[0086]

[0087] In Table 2, * represents that the p value of significance analysis is <0.05.

[0088] The present application has been described in detail above. For those skilled in the art, the present application can be implemented in a wider range under equivalent parameters, concentrations and conditions without departing from the purpose and scope of the present application, and without unnecessary experiments. Although the present application gives a special example, it should be understood that the present application can be further improved. In summary, according to the principle of the present application, the present application is intended to include any change, use or improvement of the present application, including changes made by conventional techniques known in the art, which deviates from the scope disclosed in the present application.

Claims

1. Use of a substance for detecting a wheat molecular marker in detecting or assisting in detecting drought resistance of wheat; the wheat molecular marker is a nucleotide corresponding to position 642 and / or 643 of SEQ ID No. 1 in the sequence listing in the wheat genome, the nucleotide corresponding to position 642 of SEQ ID No. 1 in the sequence listing in the wheat genome is G or C, and the nucleotide corresponding to position 643 of SEQ ID No. 1 in the sequence listing in the wheat genome is C or T.

2. Use according to claim 1, characterized in that: The substance for detecting the wheat molecular marker is a primer pair of the primer of the name TaGATA-2B -Primer or a complete set of reagents for detecting the wheat molecular marker The TaGATA-2B - Primer consists of two single-stranded DNAs represented by SEQ ID No. 4 and SEQ ID No. 5 in the sequence listing; The kit for detecting the wheat molecular marker comprises TaGATA-2B- Primer- APa LI is a primer pair, and the TaGATA-2B- Primer- APa LI is composed of two single-stranded DNAs shown in SEQ ID No. 2 and SEQ ID No. 3 in the sequence listing.

3. Use according to claim 2, characterized in that: The kit of parts also comprises a restriction enzyme APa LI.

4. The use according to any one of claims 1-3, wherein: the drought resistance of a homozygous wheat in which the nucleotide corresponding to position 642 of SEQ ID No. 1 in the sequence listing in the genome is G is greater than or is expected to be greater than the drought resistance of a homozygous wheat in which the nucleotide corresponding to position 642 of SEQ ID No. 1 in the sequence listing in the genome is C; the drought resistance of a homozygous wheat in which the nucleotide corresponding to position 643 of SEQ ID No. 1 in the sequence listing in the genome is C is greater than or is expected to be greater than the drought resistance of a homozygous wheat in which the nucleotide corresponding to position 643 of SEQ ID No. 1 in the sequence listing in the genome is T; the drought resistance of a homozygous wheat in which the nucleotides corresponding to positions 642 and 643 of SEQ ID No. 1 in the sequence listing in the genome are G and C, respectively, is greater than or is expected to be greater than the drought resistance of a homozygous wheat in which the nucleotides corresponding to positions 642 and 643 of SEQ ID No. 1 in the sequence listing in the genome are C and T, respectively.

5. A method for detecting or assisting in detecting drought resistance of wheat, comprising detecting the wheat molecular marker described in claim 1, and determining the drought resistance of the wheat according to the following method: the drought resistance of a homozygous wheat in which the nucleotide corresponding to position 642 of SEQ ID No. 1 in the sequence listing in the genome is G is greater than or is expected to be greater than the drought resistance of a homozygous wheat in which the nucleotide corresponding to position 642 of SEQ ID No. 1 in the sequence listing in the genome is C; the drought resistance of a homozygous wheat in which the nucleotide corresponding to position 643 of SEQ ID No. 1 in the sequence listing in the genome is C is greater than or is expected to be greater than the drought resistance of a homozygous wheat in which the nucleotide corresponding to position 643 of SEQ ID No. 1 in the sequence listing in the genome is T; the drought resistance of a homozygous wheat in which the nucleotides corresponding to positions 642 and 643 of SEQ ID No. 1 in the sequence listing in the genome are G and C, respectively, is greater than or is expected to be greater than the drought resistance of a homozygous wheat in which the nucleotides corresponding to positions 642 and 643 of SEQ ID No. 1 in the sequence listing in the genome are C and T, respectively.

6. The method of claim 5, wherein: detecting the wheat molecular marker described in claim 1 is performed using the substance for detecting a wheat molecular marker described in any one of claims 1-3.

7. Any one of the following uses: X1) the wheat molecular marker described in claim 1 in breeding for drought resistance of wheat; X2) the wheat molecular marker described in claim 1 in detecting or assisting in detecting drought resistance of wheat; X3) Use of the substance for detecting the molecular marker of wheat in claim 2 or 3 in drought-resistant breeding of wheat; X4) Use of the substance for detecting the molecular marker of wheat in claim 2 or 3 in preparing a product for drought-resistant breeding of wheat; X5) Use of the substance for detecting the molecular marker of wheat in any of claims 1-3 in preparing a product for detecting or assisting in detecting drought resistance of wheat; X6) Use of the method in claim 5 or 6 in drought-resistant breeding of wheat; X7) Use of the substance for detecting the nucleotides corresponding to the 642nd and 643rd nucleotides of SEQ ID No. 1 in the sequence listing in the genome of wheat in breeding drought-resistant wheat; X8) Use of the substance for detecting the nucleotides corresponding to the 642nd and 643rd nucleotides of SEQ ID No. 1 in the sequence listing in the genome of wheat in preparing a product for breeding drought-resistant wheat.

8. A method for breeding wheat, comprising I, II or III: I. detecting the nucleotides corresponding to the 642nd and 643rd nucleotides of SEQ ID No. 1 in the sequence listing in the genome of wheat, and selecting wheat in which the nucleotides corresponding to the 642nd and 643rd nucleotides of SEQ ID No. 1 in the sequence listing in the genome of wheat are G and C, respectively, as a parent for breeding; II. detecting the nucleotide corresponding to the 642nd nucleotide of SEQ ID No. 1 in the sequence listing in the genome of wheat, and selecting wheat in which the nucleotide corresponding to the 642nd nucleotide of SEQ ID No. 1 in the sequence listing in the genome of wheat is G as a parent for breeding; III. detecting the nucleotide corresponding to the 643rd nucleotide of SEQ ID No. 1 in the sequence listing in the genome of wheat, and selecting wheat in which the nucleotide corresponding to the 643rd nucleotide of SEQ ID No. 1 in the sequence listing in the genome of wheat is C as a parent for breeding.

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