A cutting method for Eurya chinensis

Abstract

The invention discloses a cutting method of Eurya chinensis, and belongs to the technical field of Eurya chinensis cutting. The invention provides a cutting method of Eurya chinensis, including preparing cutting branches, processing cutting branches, arranging seedling beds, cutting and post-management. In August, the invention selects semi-lignified branches of the current year to cut cutting branches, and disinfects the cutting branches and soaks them in a root growth promoting agent solution composed of indolebutyric acid, naphthylacetic acid and brassinolide for root growth promotion treatment, and sprays 500 times of wood vinegar on the seedling bed to adjust the soil pH before cutting, sprays iron sulfate solution for disinfection, and sprays 1000 times of mineral source humic acid diluted on the leaves after cutting, covers plastic film and sunshade net, and the above steps are combined together to form a cutting method suitable for Guizhou hairy Eurya chinensis and Gang Eurya chinensis, and the cutting rooting rate of Guizhou hairy Eurya chinensis and Gang Eurya chinensis is significantly improved through the coordinated cooperation of the above steps.

Description

Technical Field

[0001] The invention relates to the technical field of forest tree cuttings, in particular to an Eurya chinensis cutting method. Background Art

[0002] Eurya has many values, including medicinal, honey, cut flower and ornamental, and is a type of plant with great development prospects. Eurya kueichowensis and Eurya groffii belong to the genus Eurya of the Pentaphylacaceae family. Most of them are evergreen shrubs or small trees, unisexual and dioecious. There are 155 species, 7 subspecies, 16 variants and 1 variant of Eurya recorded worldwide. Currently, there are 88 species and 12 variants recorded in my country, and about 29 species in Guizhou, which has rich Eurya resources. Eurya kueichowensis and Eurya groffii are the main honey trees in Jinping, Chishui and Luodian, Guizhou. They have a large amount of nectar and a long nectar flow period. They are also important winter nectar plants in the mountainous areas of Guizhou Province. Eurya honey is known as "tribute honey" and has excellent honey quality. It is a rare material basis for the beekeeping industry. However, in recent years, due to factors such as the slow growth of Eurya, extremely low seed germination rate under natural conditions and wanton felling, wild Eurya resources have become increasingly scarce, and there is an urgent need to conduct research on efficient cultivation techniques for Eurya.

[0003] There are many seeds of Eurya guizhouensis and Eurya ganxiong, but their germination rate is extremely low and the seedlings grow slowly. Cuttings have the advantages of easy material collection, fast seedling formation and early flowering. In the research report on the cutting propagation of Eurya plants, the rooting liquids selected were: 200 mg / kg ABT rooting powder for Eurya emarginata, 1000 mg / L IBA for Eurya muricata, 1000 mg / L NAA for Eurya japonica and 2000 mg / L IBA for Eurya nitida; the existing Eurya cutting method is mainly aimed at the main tree species in the north and coastal areas. The growth environment and growth characteristics of Eurya emarginata are quite different from those of Eurya guizhouensis and Eurya gangyi, which are mainly grown in Guizhou. The cutting method is not suitable for Eurya guizhouensis and Eurya gangyi. The rooting rate of cuttings of Eurya guizhouensis and Eurya gangyi using the existing cutting method is very low. At present, there are no relevant research reports on the cutting propagation of Eurya kweichowii and Eurya gangyi. It is very necessary to develop a cutting method for Eurya kweichowii and Eurya gangyi that can improve the rooting rate of both. Summary of the invention

[0004] The object of the present invention is to provide a cutting method for Eurya chinensis to solve the problems existing in the above-mentioned prior art.

[0005] To achieve the above object, the present invention provides the following solutions:

[0006] One of the technical solutions of the present invention: a cutting method for Eurya chinensis, comprising the following steps:

[0007] (1) Prepare cuttings: Select semi-lignified branches of the current year and cut them into 10-13 cm long cuttings;

[0008] (2) Treating the cuttings: Disinfecting and promoting rooting of the cuttings; disinfecting by soaking in a 0.5 wt % carbendazim solution for 6 min, and promoting rooting by soaking in a rooting agent solution for 12 h; the rooting agent solution is a mixed solution of indolebutyric acid (IBA), naphthylacetic acid (NAA) and brassinolide (BL);

[0009] (3) Preparing the seedbed: After weeding, the sandy land is deep-ploughed and finely turned, and after ridges are formed into a seedbed, 500 times wood vinegar is sprayed to adjust the soil pH to 5.0-5.5, and ferrous sulfate solution is sprayed for disinfection, and watered thoroughly the night before cutting;

[0010] (4) Cutting: Dig holes, put in cuttings, press firmly, water the base, spray 1000 times diluted mineral source humic acid on the leaves, insert arch poles, cover with plastic film and shade net;

[0011] (5) Post-management: After cutting, spray the leaves with 1000 times diluted mineral humic acid for 2-3 times in a row, with an interval of 2 days between each spray.

[0012] Furthermore, the top of the cutting branch is cut flatly, the bottom is cut obliquely, and 2-3 leaves are left on the top.

[0013] Furthermore, the root growth promoting agent solution is composed of 90-120 mg / L indolebutyric acid + 20-30 mg / L naphthaleneacetic acid + 0.1 mg / L brassinolide.

[0014] Furthermore, the composition of the root growth promoting agent solution is preferably 105 mg / L indolebutyric acid + 25 mg / L naphthaleneacetic acid + 0.1 mg / L brassinolide.

[0015] Furthermore, the Eurya is Eurya guizhouensis or Eurya gangyi.

[0016] Furthermore, the time of cutting is August.

[0017] Furthermore, the diameter of the current year semi-lignified branches is 5±1 mm.

[0018] Furthermore, the concentration of the ferrous sulfate solution is 1wt%.

[0019] Furthermore, the post-management also includes: if the soil is dry and cracked, lift up the plastic film and sunshade net, weed and water, then cover it tightly, and then completely lift it up in the spring of next year to allow it to grow, and carry out water and fertilizer management.

[0020] The present invention discloses the following technical effects:

[0021] The invention provides a cutting method of Eurya chinensis, comprising preparing cutting branches, treating cutting branches, arranging seedling beds, cutting and post-management. In the invention, in August, semi-lignified branches of the current year are selected to cut cutting branches, and the cutting branches are disinfected and soaked in a root growth promoting agent solution composed of indolebutyric acid, naphthylacetic acid and brassinolide for root growth promotion treatment, and before cutting, 500 times of wood vinegar is sprayed on the seedling bed to adjust the soil pH, and iron sulfate solution is sprayed for disinfection, and after cutting, 1000 times of mineral source humic acid is sprayed on the leaves, and plastic film and sunshade net are covered, and the above steps are combined to form a cutting method suitable for Eurya kiwanensis and Eurya kangyanensis, and the cutting rooting rate of Eurya kiwanensis and Eurya kangyanensis is significantly improved through the coordinated cooperation of the above steps.

[0022] Through further research, the present invention found that by selecting the cutting time as August, selecting semi-lignified branches with a branch diameter of 5 mm (±1 mm), and selecting the composition of the root promoter solution as 105 mg / L indolebutyric acid + 25 mg / L naphthaleneacetic acid + 0.1 mg / L brassinolide, the best cutting rooting rate of Eurya guizhouensis and Eurya tangensis can be achieved. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0024] Figure 1 The rooting conditions of the cuttings treated in different root-promoting solutions in Example 1;

[0025] Figure 2 The growth status of the cutting roots in different months in Example 2. DETAILED DESCRIPTION

[0026] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0027] It should be understood that the terms described in the present invention are only for describing a particular embodiment and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0028] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0029] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

[0030] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0031] The Eurya species used for cuttings in the following examples are Eurya kiwanensis and Eurya kangensis. The cutting base is located in Fengting Town, Luodian County, Qiannan Prefecture, Guizhou Province, and the cutting branches are collected in Bianyang Town, Luodian County.

[0032] Example 1 Effects of root-promoting solutions of different components and concentrations on the rooting rate of Eurya chinensis branches

[0033] (1) Preparation of cuttings: In October 2021, semi-lignified Eurya guianensis and Eurya gangyi branches of the same year were picked in Bianyang Town, Luodian County, Guizhou Province. The branch diameter was 5 mm (± 1 mm) and cut into 10-13 cm cuttings. The top of the cuttings was cut flat, leaving 2-3 leaves, and the bottom was cut obliquely. 1400 cuttings were cut from each of Eurya guianensis and Eurya gangyi.

[0034] (2) Treatment of cuttings: Bundle the cuttings into bunches of 50 with rubber bands, soak them in a 0.5 wt% carbendazim solution for 6 min for disinfection, and then soak them in root-promoting solutions of different components and concentrations shown in Table 1 for 12 h. Treat 100 branches of each variety with each root-promoting solution.

[0035] (3) Preparing the seedbed: After weeding, the sandy land is deep-ploughed and finely turned to form a 1-meter-wide seedbed. 500 times wood vinegar is sprayed to adjust the soil pH to 5.0-5.5, and 1 wt% ferrous sulfate solution is sprayed for disinfection. The soil is then watered thoroughly the night before cutting.

[0036] (4) Cuttings: Use a wooden stick twice as thick as the cutting branches to dig holes, put the cutting branches in, press firmly, water the base, and spray the leaves with 1000 times diluted mineral source humic acid. Then insert the arch pole and cover with plastic film and shade net.

[0037] (5) Late management: Spray the leaves with 1000 times diluted mineral source humic acid on the 3rd and 5th days after cutting (the day of cutting is recorded as the 1st day). If the soil is dry and cracked in the later stage, lift the plastic film and shade net to weed and water, then cover it tightly. In January 2022, the rooting rate (the number of rooted branches under this treatment / the total number of branches under this treatment × 100%) will be counted, and in spring, it will be completely lifted up to allow it to grow, and water and fertilizer management will be carried out. The rooting rate results are shown in Table 1:

[0038] Table 1 Rooting rate of cuttings of Eurya guizhouensis and Eurya tangutica treated with root-promoting solutions of different components and concentrations

[0039]

[0040] Figure 1 The rooting conditions of cuttings treated with different root-promoting solutions (A. Rooting condition of water treatment, B. The rooting conditions of the treatments were as follows: C. The rooting conditions of the treatments were as follows: ⑧, D. The rooting conditions of the treatments were as follows: ②, E. The rooting conditions of the treatments were as follows: ③, and F. The rooting conditions of the treatments were as follows: Table 1 and Figure 1 The results showed that the rooting rate of Eurya guizhouensis and Eurya tangensis in treatment ③ was the highest, the best rooting agent combination was IBA+NAA+BL, the root growth state was the best, and the best rooting agent combination and the best concentration were consistent. Comparing the rooting rates after treatments ①②③④⑤, it was found that the best concentration of IBA was 105mg / L, comparing the rooting rates after treatments ③⑥⑦, it was found that the best concentration of NAA was 25mg / L, and comparing the rooting rates after treatments ③⑧, it was found that the best concentration of BL was 0.1mg / L.

[0041] Example 2 Effect of different cutting time and cutting branch diameter on the rooting rate of Eurya chinensis branches

[0042] Cutting experiments of Eupatorium guizhouensis and Eupatorium gangyi were carried out in March, May, August and October 2022.

[0043] (1) Prepare cuttings: Select semi-lignified branches of the current year and cut them into 10-13 cm long cuttings. Cut the top of the cuttings flat, leaving 2-3 leaves, and cut the bottom obliquely. Cut 400 branches of Eurya guizhouensis and Eurya gansuensis each month, including 100 branches with a diameter of 2 mm (±1 mm), 100 branches with a diameter of 5 mm (±1 mm), 100 branches with a diameter of 8 mm (±1 mm), and 100 branches with a diameter of 11 mm (±1 mm).

[0044] (2) Treatment of cuttings: Use rubber bands to bundle cuttings into bunches of 50 branches, soak them in a 0.5wt% carbendazim solution for 6 min to disinfect them, and then soak them in a root growth promoter solution (IBA 105mg / L+NAA 25mg / L+BL 0.1mg / L) for 12 h.

[0045] (3) Preparing the seedbed: After weeding, the sandy land is deep-ploughed and finely turned to form a 1-meter-wide seedbed. 500 times wood vinegar is sprayed to adjust the soil pH to 5.0-5.5, and 1 wt% ferrous sulfate solution is sprayed for disinfection. The soil is then watered thoroughly the night before cutting.

[0046] (4) Cuttings: Use a wooden stick twice as thick as the cutting branches to dig holes, put the cutting branches in, press firmly, water the base, and spray the leaves with 1000 times diluted mineral source humic acid. Then insert the arch pole and cover with plastic film and shade net.

[0047] (5) Management in the later stage: Spray the leaves with 1000 times diluted mineral source humic acid on the 3rd and 5th days after cutting (the day of cutting is counted as the 1st day). If the soil is dry and cracked in the later stage, open the plastic film and shade net, weed and water, and then cover it tightly. Count the rooting rate after 3 months, and completely open it in the spring of the next year to let it grow, and manage water and fertilizer. The rooting rate results are shown in Table 2:

[0048] Table 2 Effects of different cutting time and cutting branch diameter on rooting rate

[0049]

[0050]

[0051] Figure 2 The growth status of the root system of cuttings in different months (A. Guizhou Eurya cuttings in August, B. Guizhou Eurya cuttings in October, C. Gangyi cuttings in October, D. Gangyi cuttings in August). According to the statistical results in Table 2 and Figure 2From the root growth status, it can be seen that the most suitable cutting months and the best cutting branch diameters of Eurya guizhouensis and Eurya gansuensis are relatively consistent. The cutting rooting rates in August and October are higher than those in March and May, among which the cutting rooting rate in August is generally higher than that in October. August is the most suitable time for cutting. This may be related to the temperature after cutting and the state of the cutting seedlings. Since the temperature is relatively low after cutting in March, and the semi-lignified degree of the branches grown in March is insufficient, the rooting rate is relatively low; the temperature is relatively low after cutting in May, and the semi-lignified degree of some branches is insufficient, which is not conducive to rooting; the branches in August and October are all semi-lignified branches grown in the same year, and their cutting branches are in the best state. In addition, the high temperature geothermal energy in August-September promotes the rooting of the branches in August-December, and the temperature is suitable for root growth, so the root system is thick and dense ( Figure 2 ), the survival rate is higher after winter. The geothermal temperature is slightly lower after cutting in October, the root system is thinner, and the survival rate is slightly lower after winter. Therefore, from the perspective of rooting rate and root growth, the most suitable cutting time for Eupatorium guizhouensis and Eupatorium gangyi is August.

[0052] The best cuttings for Eurya guizhouensis and Eurya tangensis are relatively consistent, both of which are semi-lignified and grown in the same year with a diameter of 5mm (±1mm).

[0053] In summary, semi-lignified branches with a diameter of 5mm (±1mm) should be selected for cuttings of Eurya chinensis (Eurya guizhouensis and Eurya gangyi) in August.

[0054] Comparative Example 1

[0055] (1) Preparation of cuttings: In August 2022, select semi-lignified Eurya guianensis and Eurya gangyi grown in the current year with a branch diameter of 5 mm (± 1 mm) and cut into 10-13 cm cuttings. Cut the top of the cuttings flat, leaving 2-3 leaves, and cut the bottom obliquely. Cut 100 cuttings of each of Eurya guianensis and Eurya gangyi.

[0056] (2) Treatment of cuttings: Use rubber bands to bundle cuttings into bunches of 50 branches, soak them in a 0.5wt% carbendazim solution for 6 min to disinfect them, and then soak them in a root growth promoter solution (IBA 105mg / L + NAA 25mg / L + BL 0.1mg / L) for 12h.

[0057] (3) Preparing the seedbed: After weeding, the sandy land is deep-ploughed and finely turned to form a 1-meter-wide seedbed. 500 times wood vinegar is sprayed to adjust the soil pH to 5.0-5.5, and 1 wt% ferrous sulfate solution is sprayed for disinfection. The soil is then watered thoroughly the night before cutting.

[0058] (4) Cuttings: Use a wooden stick that is twice as thick as the cutting branch to dig a hole, put the cutting branch in, press it firmly, water the base, spray clean water on the leaves, insert the arch pole, and cover with plastic film and shade net.

[0059] (5) Management in the later stage: spray the leaves with clean water on the 3rd and 5th days after cutting (the day of cutting is counted as the 1st day). If the soil is dry and cracked in the later stage, lift up the plastic film and shade net, weed and water, and then cover it tightly. Count the rooting rate after 3 months, and completely lift up the net in the spring of the next year to let it grow, and manage water and fertilizer. The rooting rate results are shown in Table 3:

[0060] Table 3 Rooting rate of Eurya chinensis sprayed with water on leaves

[0061]

[0062] The cutting test of this comparative example was carried out simultaneously with the cutting test carried out in August in Example 2. The rooting rate was slightly lower than the rooting rate (80% for Eupatorium guizhouensis and 82% for Eupatorium roxburghii) of Example 2 with 1000 times of mineral source humic acid sprayed on the leaves under the same conditions, indicating that 1000 times of mineral source humic acid sprayed on the leaves promoted the rooting of Eupatorium guizhouensis and Eupatorium roxburghii in cutting propagation.

[0063] Comparative Example 2

[0064] (1) Prepare cuttings: In August 2022, select semi-lignified Eurya chinensis branches of the current year with a branch diameter of 5 mm (± 1 mm) and cut them into 10-13 cm cuttings. Cut the top of the cuttings flat, leaving 2-3 leaves, and cut the bottom obliquely.

[0065] (2) Treatment of cuttings: Bundle 50 cuttings with rubber bands, soak them in a 0.5wt% carbendazim solution for 6 min to disinfect, and then soak them in a root growth promoter solution (IBA 105mg / L+NAA 25mg / L+BL 0.1mg / L) for 12h.

[0066] (3) Preparing the seedbed: After weeding, the sandy land is deep-ploughed and finely turned to form a 1-meter-wide seedbed. 500 times wood vinegar is sprayed to adjust the soil pH to 5.0-5.5, and 1 wt% ferrous sulfate solution is sprayed for disinfection. The soil is then watered thoroughly the night before cutting.

[0067] (4) Cuttings: Use a wooden stick that is twice as thick as the cutting branch to dig a hole, put the cutting branch in, press it firmly, water it, spray 1000 times the mineral source humic acid on the leaves, insert an arch pole, and cover it with plastic film and shade net.

[0068] (5) Management in the later stage: spray 1000 times of mineral source humic acid on the 3rd and 5th day after cutting (the day of cutting is recorded as the 1st day). If the soil is dry and cracked in the later stage, open the plastic film and shade net, weed and water, and then cover it tightly. The rooting rate was counted after 3 months, and the rooting rate was 48%. It can be seen that the cutting method of the present invention is not effective for the cutting propagation of Eupatorium truncatum.

[0069] The cutting test of this comparative example and the cutting test carried out in August in Example 2 were carried out simultaneously.

[0070] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A cutting method for Eurya chinensis, characterized in that: The following steps are involved: (1) Prepare cuttings: Select semi-lignified branches of the current year and cut them into 10-13 cm long cuttings; (2) Treating the cuttings: Disinfecting and promoting root growth of the cuttings; the cuttings are disinfected by soaking in a 0.5 wt % carbendazim solution for 6 min, and the root growth promotion treatment is soaked in a root growth promoter solution for 12 h; the root growth promoter solution is a mixed solution of indolebutyric acid, naphthylacetic acid and brassinolide; (3) Preparing the seedbed: After weeding, the sandy land is deep-ploughed and finely turned, and after ridges are formed into a seedbed, 500 times wood vinegar is sprayed to adjust the soil pH to 5.0-5.5, and ferrous sulfate solution is sprayed for disinfection, and watered thoroughly the night before cutting; (4) Cutting: Dig holes, put in cuttings, press firmly, water the base, spray 1000 times diluted mineral source humic acid on the leaves, insert arch poles, cover with plastic film and shade net; (5) Post-management: Spray the leaves with 1000-fold diluted mineral-based humic acid on the 3rd and 5th days after cutting; The top of the cutting branch is cut flatly, the bottom is cut obliquely, and 2-3 leaves are left on the top; The root growth promoting agent solution is composed of 105 mg / L indolebutyric acid + 25 mg / L naphthaleneacetic acid + 0.1 mg / L brassinolide; The Eurya is Eurya guizhouensis or Eurya kangensis; The time of the cutting is August; The diameter of the semi-lignified branches of the current year is 5±1 mm; The concentration of the ferrous sulfate solution is 1 wt %.

Images