Bupleurum tissue culture device and culture method

Abstract

The present application relates to the technical field of bupleurum tissue culture, and discloses a bupleurum tissue culture device and culture method, which comprises an exhaust main mechanism, a cabinet door mechanism is movably installed on the surface of the exhaust main mechanism, a pump liquid mechanism is installed in the cabinet door mechanism, constant temperature heating mechanisms are installed inside the two sides of the exhaust main mechanism, and a light source mechanism is installed inside the back side of the exhaust main mechanism. The present application can pump the black oily liquid in the cabinet door mechanism through the pump liquid mechanism when observing, so that the inside of the liquid guide hollow glass plate is filled with nitrogen, the light transmission of the liquid guide hollow glass plate is ensured, and observation can be performed. When culturing, the inside of the liquid guide hollow glass plate is filled with black oily liquid pushed by the pump liquid mechanism, so that external light is difficult to penetrate, the device is ensured to be illuminated by the internal light source mechanism, so that light pollution from the outside is prevented from shining into the device during non-observation time, and the control effect of internal illumination variables is improved.

Description

Technical Field

[0001] This invention relates to the field of Bupleurum tissue culture technology, specifically to a Bupleurum tissue culture device and culture method. Background Technology

[0002] Plant tissue culture is a new asexual reproduction technique developed in recent decades based on the theory of totipotency of plant cells. Broadly speaking, plant tissue culture, also known as in vitro culture, refers to the technique of isolating suitable tissues, organs, or cells (protoplasts, etc.) from a plant and, under aseptic conditions, inoculating them onto a culture medium containing various nutrients and plant hormones to obtain regenerated complete plants or produce other economically valuable products. In a narrower sense, tissue culture refers to the cultivation of regenerated plants using various plant tissues, such as cambium, parenchyma, mesophyll, and endosperm. It also refers to the cultivation of callus tissue generated from various organs during the culture process, which then undergoes redifferentiation to form regenerated plants. Plant tissue culture technology can be used to cultivate economic crops and traditional Chinese medicinal materials, such as periwinkle and purslane. Bupleurum, a traditional Chinese medicine, is a herb included in the Chinese Pharmacopoeia. The medicinal part is the dried root of Bupleurum chinense or Bupleurum stenoptera, which are plants of the Apiaceae family. It is harvested in spring and autumn, and the stems, leaves and soil are removed before drying. Bupleurum is a commonly used diaphoretic. Other names include ground smoke, mountain vegetable, mushroom grass, and firewood grass. It is bitter and slightly cold in nature, and enters the liver and gallbladder meridians. It has the effects of harmonizing the exterior and interior, soothing the liver and raising yang. It is used for colds with fever, alternating chills and fever, malaria, liver qi stagnation, chest and rib distension and pain, rectal prolapse, uterine prolapse, and irregular menstruation.

[0003] In plant tissue culture, a culture cabinet is generally used for constant temperature placement. To facilitate observation, a transparent observation window is usually set up in the culture cabinet for observation from the outside. However, the light-transmitting material allows light pollution from the outside to shine into the device for a long time during non-observation periods, which can easily conflict with the internal light source and greatly affect the control of internal light variables. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention provides a Bupleurum tissue culture device and method, which solves the problems mentioned in the background.

[0005] The present invention provides the following technical solution: a Bupleurum tissue culture device, comprising: an exhaust main body mechanism, a cabinet door mechanism movably mounted on the surface of the exhaust main body mechanism, a liquid pump mechanism installed inside the cabinet door mechanism, a constant temperature heating mechanism installed inside both sides of the exhaust main body mechanism, a light source mechanism installed inside the back side of the exhaust main body mechanism, and an air intake purification mechanism installed on the top of the exhaust main body mechanism.

[0006] Preferably, the exhaust system includes a cabinet, a base box, an air washing tank, an exhaust fan, a shelf, a mounting bracket, an exhaust box, a duct hose, and a sealing ring. The base box is fixedly connected to the bottom of the cabinet, the air washing tank is slidably connected to the inside of the base box, the exhaust fan is fixedly installed on the surface of the base box, the shelf is fixedly connected to the inside of the cabinet, the mounting bracket is fixedly connected between the cabinet and the base box, the exhaust box is fixedly connected to the inside of the mounting bracket, the duct hose is fixedly connected to the bottom of the exhaust box, and the sealing ring is fixedly connected to the surface of the cabinet. The air washing tank is also provided with disinfectant.

[0007] Preferably, the cabinet door mechanism includes a cabinet door body, an air storage chamber, a liquid storage chamber, an upper sealing plate, and a lower sealing plate. The cabinet door body is rotatably connected to the front of the cabinet body, and a cabinet lock is installed between the cabinet body and the cabinet door body. The air storage chamber and the liquid storage chamber are both opened inside the cabinet door body, and the air storage chamber is located above the liquid storage chamber. The upper sealing plate is fixedly connected to the inner wall of the air storage chamber, and the lower sealing plate is fixedly connected to the inner wall of the liquid storage chamber.

[0008] Preferably, the cabinet door mechanism further includes a liquid-guiding hollow glass plate, a heat-insulating hollow glass plate, liquid-guiding pipes, and spring-loaded one-way valves. The liquid-guiding hollow glass plate and the heat-insulating hollow glass plate are both fixedly connected inside the cabinet door body, and the heat-insulating hollow glass plate is located on the side of the cabinet door body closer to the cabinet. There are four liquid-guiding pipes, with each pair of liquid-guiding pipes forming a group, and the two groups of liquid-guiding pipes are fixedly connected to the upper and lower sides of the liquid-guiding hollow glass plate, respectively. The spring-loaded one-way valves are fixedly connected to one end of each of the four liquid-guiding pipes, and the two spring-loaded one-way valves in each group are a drain one-way valve and an inlet one-way valve, respectively.

[0009] Preferably, the pumping mechanism includes an isolation frame, an electromagnet, an iron bar, a support bar, a pumping chamber, a pumping piston, a piston rod, and a return spring. The isolation frame is fixedly connected inside the liquid storage chamber, the electromagnet is fixedly installed inside the liquid storage chamber, the support bar is fixedly connected to the bottom of the isolation frame, the pumping chamber is opened through the inside of the isolation frame, the pumping piston is slidably connected inside the pumping chamber, the piston rod is fixedly inserted into the bottom of the pumping piston, and the surface of the piston rod is slidably connected to the inner wall of the support bar, the iron bar is fixedly connected to the bottom end of the piston rod, the return spring is movably installed between the pumping piston and the support bar, and the space between the pumping piston and the liquid-guiding hollow glass plate is filled with a black oily liquid, and the space between the black oily liquid and the gas storage chamber is filled with nitrogen gas.

[0010] Preferably, the constant temperature heating mechanism includes a heating tank, a side cover plate, a side insulation plate, a heat-conducting plate, an electric heating rod, a temperature controller, an oil pump, a return pipe, and a drain pipe. The heating tank is respectively opened on both sides of the cabinet. The side cover plate and the side insulation plate are fixedly connected to the inside of the heating tank. The heat-conducting plate is fixedly connected to the inner wall of the heating tank. The electric heating rod and the temperature controller are fixedly installed inside the heating tank. The oil pump is fixedly installed at the bottom of the mounting plate. The return pipe is fixedly installed between the input end of the oil pump and the heating tank. The drain pipe is fixedly installed between the output end of the oil pump and the heating tank. The inside of the heating tank is filled with heat-conducting liquid.

[0011] Preferably, the light source mechanism includes a back insulation board, a lamp housing, a supplementary light strip, an ultraviolet light strip, and a light-transmitting plate. The back insulation board and the lamp housing are both fixedly connected to the inside of the back side of the cabinet. The supplementary light strip and the ultraviolet light strip are both fixedly installed inside the lamp housing. The light-transmitting plate is fixedly connected to the inner wall of the lamp housing.

[0012] Preferably, the air intake purification mechanism includes a top frame, a heat insulation plate, a side mounting groove, a top plate, a support mesh, a filter sponge, and an electric heating block. The top frame is fixedly connected to the top of the cabinet, the heat insulation plate is fixedly connected to the bottom of the top frame, the side mounting groove is opened on one side of the top frame, the top plate is fixedly connected to the inner wall of the top of the top frame, the support mesh and the filter sponge are both fixedly connected inside the side mounting groove, and the electric heating block is fixedly connected to the inner wall of the top plate.

[0013] Preferably, the air intake purification mechanism further includes a heat-conducting block, an air intake parallel connection cylinder, heat-conducting pipes, an exhaust pipe, and a support plate. The heat-conducting block is fixedly connected to both sides of the heating block. The air intake parallel connection cylinder is fixedly connected to the inner wall of the side mounting groove. The exhaust pipe is fixedly connected between the top frame and the heat insulation plate. There are multiple heat-conducting pipes, and all of them are fixedly connected between the air intake parallel connection cylinder and the exhaust pipe. All of them penetrate the interior of the heat-conducting block. The support plate is fixedly sleeved on the surface of the heat-conducting pipes.

[0014] A method for culturing Bupleurum chinense tissue based on a Bupleurum chinense tissue culture device includes:

[0015] Step S1: The ultraviolet lamp strip is powered on to emit ultraviolet light to disinfect the inside of the device;

[0016] Step S2: Power on the heating block and start the exhaust fan to circulate air inside the device for ventilation;

[0017] Step S3: Start the oil pump and turn on the heating element to gradually preheat the inside of the device to the specified temperature and then maintain the temperature.

[0018] Step S4: After disinfection for a period of time, the ultraviolet light strip is turned off, and the supplemental light strip is turned on to emit light, waiting for subsequent supplemental lighting to the plants;

[0019] Step S5: After opening the cabinet door, place the culture bottle with the planted Bupleurum tissue on the shelf, then close the cabinet door and lock the cabinet lock;

[0020] Step S6: When it is necessary to observe the growth of Bupleurum chinense from the outside, the electromagnet is energized to draw out the black oily liquid from the liquid-conducting hollow glass plate, ensuring that the liquid-conducting hollow glass plate is transparent to light.

[0021] Step S7: After observation, the electromagnet is de-energized, and the black oily liquid is pumped back into the liquid-conducting hollow glass plate and refilled, ensuring that the liquid-conducting hollow glass plate is opaque.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] 1. This invention, through its exhaust mechanism, cabinet door mechanism, pump mechanism, constant temperature heating mechanism, light source mechanism, and air intake purification mechanism, enables the pump mechanism to draw black oily liquid from inside the cabinet door mechanism during observation, filling the hollow glass plate with nitrogen gas and ensuring light transmission for observation. During cultivation, the pump mechanism pushes black oily liquid to fill the hollow glass plate, making it difficult for external light to pass through. This ensures that the device can be illuminated through the internal light source mechanism, thus preventing external light pollution from entering the device during non-observation periods and improving the control over internal light variables.

[0024] 2. This invention, through the installation of a cabinet, base box, gas washing tank, exhaust fan, shelf, mounting rack, exhaust box, air duct and sealing ring, can place culture bottles while also forming an isolation through the disinfectant inside the gas washing tank, preventing pathogens from entering the device through the air duct.

[0025] 3. This invention, through the cabinet door body, air storage chamber, liquid storage chamber, upper sealing plate, lower sealing plate, liquid-guiding hollow glass plate, heat-insulating hollow glass plate, liquid guiding pipe and spring one-way valve, can change the light transmission effect by controlling the black oily liquid and nitrogen gas inside the liquid-guiding hollow glass plate.

[0026] 4. This invention, through the setting of a top frame, heat insulation plate, side mounting groove, top plate, support mesh, filter sponge, electric heating block, heat conducting block, air inlet connecting cylinder, heat conducting pipe, exhaust pipe and support plate, can heat and sterilize the air entering the device before cooling it, thereby purifying the air and preventing pathogens from easily entering the device. Attached Figure Description

[0027] Figure 1 This is a schematic diagram of the structure of the present invention;

[0028] Figure 2 This is a main sectional view of the present invention;

[0029] Figure 3 This is a side sectional view of the present invention;

[0030] Figure 4 This is a schematic diagram of the exploded structure of the present invention;

[0031] Figure 5 This is a schematic diagram of the structure at the location of the oil pump in this invention;

[0032] Figure 6 This is a side sectional view of the cabinet door mechanism of the present invention;

[0033] Figure 7 This is a schematic diagram of the exploded internal structure of the cabinet door mechanism of the present invention;

[0034] Figure 8 This is an internal sectional view of the isolation frame of the present invention;

[0035] Figure 9 This is a schematic diagram of the internal exploded structure of the air intake purification mechanism of the present invention.

[0036] In the diagram: 101. Cabinet body; 102. Base box; 103. Air washing tank; 104. Exhaust fan; 105. Shelf; 106. Mounting rack; 107. Exhaust box; 108. Air guide hose; 109. Sealing ring; 201. Cabinet door body; 202. Air storage chamber; 203. Liquid storage chamber; 204. Upper sealing plate; 205. Lower sealing plate; 206. Liquid guide hollow glass plate; 207. Heat-insulating hollow glass plate; 208. Liquid guide pipe; 209. Spring one-way valve; 301. Isolation frame; 302. Electromagnet; 303. Iron bar; 304. Support bar; 305. Liquid extraction chamber; 306. Liquid extraction piston; 307. Piston column; 308. 401. Return spring; 402. Heating groove; 403. Side cover plate; 404. Side insulation plate; 405. Heat-conducting plate; 406. Electric heating rod; 407. Thermostat; 408. Oil pump; 409. Return pipe; 500. Drain pipe; 501. Back insulation plate; 502. Lamp housing; 503. Supplemental light strip; 504. Ultraviolet light strip; 505. Light-transmitting light plate; 601. Top frame; 602. Heat insulation plate; 603. Side mounting groove; 604. Top plate; 605. Support mesh; 606. Filter sponge; 607. Electric heating block; 608. Heat-conducting block; 609. Air inlet connecting tube; 610. Heat-conducting pipe; 611. Exhaust pipe; 612. Support plate. Detailed Implementation

[0037] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0038] Please see Figure 1-9 A Bupleurum chinense tissue culture device includes: an exhaust main body mechanism; a cabinet door mechanism movably mounted on the surface of the exhaust main body mechanism; a liquid pumping mechanism installed inside the cabinet door mechanism; constant temperature heating mechanisms installed inside both sides of the exhaust main body mechanism; a light source mechanism installed inside the back side of the exhaust main body mechanism; and an air intake purification mechanism installed on the top of the exhaust main body mechanism. Through the exhaust main body mechanism, cabinet door mechanism, liquid pumping mechanism, constant temperature heating mechanism, light source mechanism, and air intake purification mechanism, during observation, the liquid pumping mechanism draws black oily liquid from inside the cabinet door mechanism, filling the hollow glass plate 206 with nitrogen gas, ensuring the hollow glass plate 206 is transparent for observation. During culture, the liquid pumping mechanism pushes black oily liquid to fill the hollow glass plate 206, making it difficult for external light to pass through. The device uses an internal light source mechanism for illumination settings, thus preventing external light pollution from entering the device during non-observation periods and improving the control of internal light variables.

[0039] The exhaust system includes a cabinet 101, a base box 102, an air washing tank 103, an exhaust fan 104, a shelf 105, a mounting bracket 106, an exhaust box 107, a flexible air duct 108, and a sealing ring 109. The base box 102 is fixedly connected to the bottom of the cabinet 101. The air washing tank 103 is slidably connected to the inside of the base box 102. The exhaust fan 104 is fixedly installed on the surface of the base box 102. The shelf 105 is fixedly connected to the inside of the cabinet 101. The mounting bracket 106 is fixedly connected between the cabinet 101 and the base box 102. The exhaust box 107 is fixedly connected to the mounting bracket 107. Inside the shelf 106, the air duct 108 is fixedly connected to the bottom of the exhaust box 107, and the sealing ring 109 is fixedly connected to the surface of the cabinet 101. The inside of the gas washing tank 103 is filled with disinfectant. Through the cabinet 101, bottom box 102, gas washing tank 103, exhaust fan 104, shelf 105, mounting shelf 106, exhaust box 107, air duct 108 and sealing ring 109, culture bottles can be placed while the disinfectant inside the gas washing tank 103 forms an isolation to prevent pathogens from entering the device through the air duct 108.

[0040] The cabinet door mechanism includes a cabinet door body 201, an air storage chamber 202, a liquid storage chamber 203, an upper sealing plate 204, and a lower sealing plate 205. The cabinet door body 201 is rotatably connected to the front of the cabinet body 101, and a cabinet lock is installed between the cabinet body 101 and the cabinet door body 201. The air storage chamber 202 and the liquid storage chamber 203 are both opened inside the cabinet door body 201, and the air storage chamber 202 is located above the liquid storage chamber 203. The upper sealing plate 204 is fixedly connected to the inner wall of the air storage chamber 202, and the lower sealing plate 205 is fixedly connected to the inner wall of the liquid storage chamber 203.

[0041] The cabinet door mechanism also includes a liquid-guiding hollow glass plate 206, a heat-insulating hollow glass plate 207, liquid-guiding pipes 208, and spring-loaded one-way valves 209. The liquid-guiding hollow glass plate 206 and the heat-insulating hollow glass plate 207 are both fixedly connected inside the cabinet door body 201, with the heat-insulating hollow glass plate 207 located on the side of the cabinet door body 201 closest to the cabinet body 101. There are four liquid-guiding pipes 208, with each pair forming a group. Two groups of liquid-guiding pipes 208 are fixedly connected to the upper and lower sides of the liquid-guiding hollow glass plate 206, respectively. Spring-loaded one-way valves 209 are fixedly connected to one end of each of the four liquid-guiding pipes 208, with each group consisting of two... The spring check valves 209 are both drainage check valves and inlet check valves. By setting the spring check valves 209, the black oily liquid and nitrogen gas are prevented from easily entering and exiting the liquid-guiding hollow glass plate 206 when the device shakes, providing a small resistance for the entry and exit of the black oily liquid and nitrogen gas. Through the cabinet door body 201, gas storage chamber 202, liquid storage chamber 203, upper sealing plate 204, lower sealing plate 205, liquid-guiding hollow glass plate 206, heat-insulating hollow glass plate 207, liquid guiding pipe 208 and spring check valves 209, the light transmission effect can be changed by controlling the black oily liquid and nitrogen gas inside the liquid-guiding hollow glass plate 206.

[0042] The pumping mechanism includes an isolation frame 301, an electromagnet 302, an iron bar 303, a support bar 304, a pumping chamber 305, a pumping piston 306, a piston rod 307, and a return spring 308. The isolation frame 301 is fixedly connected inside the storage chamber 203, the electromagnet 302 is fixedly installed inside the storage chamber 203, the support bar 304 is fixedly connected to the bottom of the isolation frame 301, the pumping chamber 305 is formed through the inside of the isolation frame 301, the pumping piston 306 is slidably connected inside the pumping chamber 305, and the piston rod 307 is fixedly inserted into the bottom of the pumping piston 306. The surface of the piston column 307 is slidably connected to the inner wall of the support bar 304. The iron bar 303 is fixedly connected to the bottom end of the piston column 307. The return spring 308 is movably installed between the liquid-drawing piston 306 and the support bar 304. The space between the liquid-drawing piston 306 and the liquid-guiding hollow glass plate 206 is filled with black oily liquid, and the space between the black oily liquid and the gas storage chamber 202 is filled with nitrogen gas. The black oily liquid filling facilitates light absorption and prevents external light from easily passing through. At the same time, it prevents light from easily getting stuck inside the liquid-guiding hollow glass plate 206 and prevents it from affecting the light transmittance of the liquid-guiding hollow glass plate 206 after it is discharged.

[0043] The constant temperature heating mechanism includes a heating tank 401, a side cover plate 402, a side insulation plate 403, a heat-conducting plate 404, an electric heating rod 405, a thermostat 406, an oil pump 407, a return pipe 408, and a drain pipe 409. The heating tank 401 is located on both sides of the cabinet 101. The side cover plate 402 and the side insulation plate 403 are fixedly connected to the inside of the heating tank 401. The heat-conducting plate 404 is fixedly connected to the inner wall of the heating tank 401. The electric heating rod 405 and the thermostat... All components 406 are fixedly installed inside the heating tank 401. The oil pump 407 is fixedly installed at the bottom of the mounting plate 106. The return pipe 408 is fixedly installed between the input end of the oil pump 407 and the heating tank 401. The drain pipe 409 is fixedly installed between the output end of the oil pump 407 and the heating tank 401. The heating tank 401 is filled with heat-conducting liquid to ensure uniform heat conduction through the circulation of the heat-conducting liquid. The electric heating rod 405 heats the tank and the temperature controller 406 ensures constant temperature.

[0044] The light source mechanism includes a back insulation board 501, a lamp housing 502, a supplementary light strip 503, an ultraviolet light strip 504, and a light-transmitting panel 505. The back insulation board 501 and the lamp housing 502 are both fixedly connected to the inside of the back side of the cabinet 101. The supplementary light strip 503 and the ultraviolet light strip 504 are both fixedly installed inside the lamp housing 502. The light-transmitting panel 505 is fixedly connected to the inner wall of the lamp housing 502 to facilitate the generation of light and sterilization.

[0045] The air intake purification mechanism includes a top frame 601, a heat insulation plate 602, a side mounting groove 603, a top plate 604, a support mesh 605, a filter sponge 606, and an electric heating block 607. The top frame 601 is fixedly connected to the top of the cabinet 101, the heat insulation plate 602 is fixedly connected to the bottom of the top frame 601, the side mounting groove 603 is opened on one side of the top frame 601, the top plate 604 is fixedly connected to the inner wall of the top of the top frame 601, the support mesh 605 and the filter sponge 606 are both fixedly connected to the inside of the side mounting groove 603, and the electric heating block 607 is fixedly connected to the inner wall of the top plate 604.

[0046] The air intake purification mechanism also includes a heat-conducting block 608, an air intake parallel connection cylinder 609, heat-conducting pipes 610, an exhaust pipe 611, and a support plate 612. The heat-conducting block 608 is fixedly connected to both sides of the heating block 607. The air intake parallel connection cylinder 609 is fixedly connected to the inner wall of the side mounting groove 603. The exhaust pipe 611 is fixedly connected between the top frame 601 and the heat insulation plate 602. Multiple heat-conducting pipes 610 are used, and all of them are fixedly connected between the air intake parallel connection cylinder 609 and the exhaust pipe 611. All tubes 610 penetrate the interior of the heat-conducting block 608, and the support plate 612 is fixedly sleeved on the surface of the heat-conducting tube 610. Through the top frame 601, heat insulation plate 602, side mounting groove 603, top plate 604, support mesh 605, filter sponge 606, electric heating block 607, heat-conducting block 608, air inlet and connecting cylinder 609, heat-conducting tube 610, exhaust pipe 611 and support plate 612, the air entering the device can be heated and sterilized before being cooled, so as to purify the air and prevent pathogens from easily entering the device.

[0047] A method for culturing Bupleurum chinense tissue based on a Bupleurum chinense tissue culture device includes:

[0048] Step S1: The UV lamp strip 504 is powered on to emit UV light to disinfect the inside of the device;

[0049] Step S2: The heating block 607 is powered on, and the exhaust fan 104 is started to circulate air inside the device. After the exhaust fan 104 is started, it generates negative pressure, causing the airflow to flow from the top to the bottom of the device. The airflow first passes through the support net 605 and the filter sponge 606 to filter dust, and then enters the heat conduction pipe 610 from the air inlet and connecting tube 609. When passing through the heat conduction block 608, the air is heated by the heat conduction of the heat conduction pipe 610, so that the pathogens in the air are killed by the high temperature. Then the air continues to flow along the heat conduction pipe 610, and is gradually cooled by the room temperature. Then the cooled air enters the exhaust pipe 611, and then enters the cabinet 101. Then it flows downward from the top of the cabinet 101, providing plant respiration inside the cabinet 101. The exhaust gas is driven downward by the negative pressure, and then enters the exhaust box 107. Then it enters the air washing tank 103 along the air guide hose 108, and then floats up from the disinfectant inside the air washing tank 103, so that the exhaust gas enters the bottom box 102, and then is discharged from the device by the exhaust fan 104.

[0050] Step S3: Start the oil pump 407 and power on the heating rod 405 to gradually preheat the inside of the device to the specified temperature and then maintain the temperature. After the oil pump 407 starts, the heat transfer liquid inside the heating tank 401 enters the oil pump 407 from the return pipe 408 and is then pumped back by the oil pump 407 from the drain pipe 409 to achieve circulation. At the same time, the heating rod 405 heats the heat transfer liquid, so that the inside of the device is gradually heated and the temperature is maintained by the temperature controller 406.

[0051] Step S4: After disinfection for a period of time, the UV light strip 504 is turned off, and the supplemental light strip 503 is turned on to emit light, waiting for subsequent supplemental lighting to the plants;

[0052] Step S5: After opening the cabinet door body 201, place the culture bottle with the planted Bupleurum tissue on the shelf 105, then close the cabinet door body 201 and lock the cabinet lock;

[0053] Step S6: When it is necessary to observe the growth of Bupleurum chinense from the outside, the electromagnet 302 is energized to draw the black oily liquid out of the liquid-conducting hollow glass plate 206, ensuring that the liquid-conducting hollow glass plate 206 is transparent to light. When the electromagnet 302 is energized, the iron bar 303 is attracted and descends. The iron bar 303 moves the liquid-drawing piston 306 down through the piston column 307, thereby drawing the black oily liquid inside the liquid-conducting hollow glass plate 206 into the liquid storage chamber 203 and the liquid drawing chamber 305, so that nitrogen fills the liquid-conducting hollow glass plate 206, thereby making the inside of the liquid-conducting hollow glass plate 206 transparent to light.

[0054] Step S7: After observation, the electromagnet 302 is de-energized, and the black oily liquid is pumped back into the liquid-conducting hollow glass plate 206 and filled, ensuring that the liquid-conducting hollow glass plate 206 is opaque. When the electromagnet 302 is de-energized, the reset spring 308 pushes the liquid-drawing piston 306 to slide upward and reset. The liquid-drawing piston 306 squeezes the black oily liquid inside the liquid-drawing chamber 305 upward, so that the inside of the liquid-conducting hollow glass plate 206 is filled with black oily liquid, thereby making the inside of the liquid-conducting hollow glass plate 206 opaque.

[0055] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A Bupleurum chinense tissue culture device, characterized in that, include: The exhaust main body mechanism has a cabinet door mechanism movably installed on its surface, a pump mechanism installed inside the cabinet door mechanism, constant temperature heating mechanisms installed inside both sides of the exhaust main body mechanism, a light source mechanism installed inside the back side of the exhaust main body mechanism, and an air intake purification mechanism installed on the top of the exhaust main body mechanism. The exhaust system includes a cabinet (101), a base box (102), an air washing tank (103), an exhaust fan (104), a shelf (105), a mounting plate (106), an exhaust box (107), an air guide hose (108), and a sealing ring (109). The base box (102) is fixedly connected to the bottom of the cabinet (101), the air washing tank (103) is slidably connected to the inside of the base box (102), and the exhaust fan (104) is fixedly installed in the base box (102). The surface of the cabinet is provided with the following: the shelf (105) is fixedly connected to the inside of the cabinet (101); the mounting plate (106) is fixedly connected between the cabinet (101) and the bottom box (102); the exhaust box (107) is fixedly connected to the inside of the mounting plate (106); the air guide hose (108) is fixedly connected to the bottom of the exhaust box (107); the sealing ring (109) is fixedly connected to the surface of the cabinet (101); and the inside of the air washing tank (103) is provided with disinfectant. The cabinet door mechanism includes a cabinet door body (201), an air storage chamber (202), a liquid storage chamber (203), an upper sealing plate (204), and a lower sealing plate (205). The cabinet door body (201) is rotatably connected to the front of the cabinet (101), and a cabinet lock is installed between the cabinet (101) and the cabinet door body (201). The air storage chamber (202) and the liquid storage chamber (203) are both opened inside the cabinet door body (201), and the air storage chamber (202) is located above the liquid storage chamber (203). The upper sealing plate (204) is fixedly connected to the inner wall of the air storage chamber (202), and the lower sealing plate (205) is fixedly connected to the inner wall of the liquid storage chamber (203). The cabinet door mechanism also includes a liquid-guiding hollow glass plate (206), a heat-insulating hollow glass plate (207), a liquid-guiding pipe (208), and a spring one-way valve (209). The liquid-guiding hollow glass plate (206) and the heat-insulating hollow glass plate (207) are both fixedly connected to the inside of the cabinet door body (201), and the heat-insulating hollow glass plate (207) is located on the side of the cabinet door body (201) close to the cabinet body (101). There are four liquid-guiding pipes (208), and every two liquid-guiding pipes (208) form a group. The two groups of liquid-guiding pipes (208) are fixedly connected to the upper and lower sides of the liquid-guiding hollow glass plate (206), respectively. The spring one-way valve (209) is fixedly connected to one end of the four liquid-guiding pipes (208), and the two spring one-way valves (209) in each group are respectively a drain one-way valve and an inlet one-way valve. The pumping mechanism includes an isolation frame (301), an electromagnet (302), an iron bar (303), a support bar (304), a pumping chamber (305), a pumping piston (306), a piston rod (307), and a return spring (308). The isolation frame (301) is fixedly connected to the inside of the storage chamber (203). The electromagnet (302) is fixedly installed inside the storage chamber (203). The support bar (304) is fixedly connected to the bottom of the isolation frame (301). The pumping chamber (305) is opened through the inside of the isolation frame (301). The pumping piston (306) is fixedly connected to the bottom of the isolation frame (301). 6) The piston column (307) is fixedly inserted into the bottom of the pumping piston (306) and the surface of the piston column (307) is slidably connected to the inner wall of the support bar (304). The iron bar (303) is fixedly connected to the bottom end of the piston column (307). The reset spring (308) is movably installed between the pumping piston (306) and the support bar (304). The space between the pumping piston (306) and the liquid-guiding hollow glass plate (206) is filled with black oily liquid, and the space between the black oily liquid and the gas storage chamber (202) is filled with nitrogen.

2. The Bupleurum chinense tissue culture device according to claim 1, characterized in that, The constant temperature heating mechanism includes a heating tank (401), a side cover plate (402), a side insulation plate (403), a heat-conducting plate (404), an electric heating rod (405), a thermostat (406), an oil pump (407), a return pipe (408), and a drain pipe (409). The heating tank (401) is respectively opened on both sides of the cabinet (101). The side cover plate (402) and the side insulation plate (403) are both fixedly connected to the inside of the heating tank (401). The heat-conducting plate (404) is fixedly connected to the side insulation plate (405). The inner wall of the heating tank (401) is fixedly installed with the heating rod (405) and the temperature controller (406) inside the heating tank (401). The oil pump (407) is fixedly installed at the bottom of the mounting plate (106). The return pipe (408) is fixedly installed between the input end of the oil pump (407) and the heating tank (401). The drain pipe (409) is fixedly installed between the output end of the oil pump (407) and the heating tank (401). The interior of the heating tank (401) is filled with heat-conducting liquid.

3. The Bupleurum chinense tissue culture device according to claim 2, characterized in that, The light source mechanism includes a back insulation board (501), a lamp housing (502), a supplementary light strip (503), an ultraviolet light strip (504), and a light-transmitting plate (505). The back insulation board (501) and the lamp housing (502) are both fixedly connected to the inside of the back side of the cabinet (101). The supplementary light strip (503) and the ultraviolet light strip (504) are both fixedly installed inside the lamp housing (502). The light-transmitting plate (505) is fixedly connected to the inner wall of the lamp housing (502).

4. The Bupleurum chinense tissue culture device according to claim 3, characterized in that, The air intake purification mechanism includes a top frame (601), a heat insulation plate (602), a side mounting groove (603), a top plate (604), a support mesh (605), a filter sponge (606), and an electric heating block (607). The top frame (601) is fixedly connected to the top of the cabinet (101), the heat insulation plate (602) is fixedly connected to the bottom of the top frame (601), the side mounting groove (603) is opened on one side of the top frame (601), the top plate (604) is fixedly connected to the inner wall of the top of the top frame (601), the support mesh (605) and the filter sponge (606) are both fixedly connected inside the side mounting groove (603), and the electric heating block (607) is fixedly connected to the inner wall of the top plate (604).

5. The Bupleurum chinense tissue culture device according to claim 4, characterized in that, The air intake purification mechanism also includes a heat-conducting block (608), an air intake parallel connection cylinder (609), a heat-conducting pipe (610), an exhaust pipe (611), and a support plate (612). The heat-conducting block (608) is fixedly connected to both sides of the heating block (607). The air intake parallel connection cylinder (609) is fixedly connected to the inner wall of the side mounting groove (603). The exhaust pipe (611) is fixedly connected between the top frame (601) and the heat insulation plate (602). There are multiple heat-conducting pipes (610), and all of them are fixedly connected between the air intake parallel connection cylinder (609) and the exhaust pipe (611). All of them penetrate the interior of the heat-conducting block (608). The support plate (612) is fixedly sleeved on the surface of the heat-conducting pipe (610).

6. A method for culturing Bupleurum chinense tissue based on the Bupleurum chinense tissue culture apparatus according to claim 5, characterized in that, include: Step S1: The ultraviolet lamp strip (504) is powered on to emit ultraviolet light to disinfect the inside of the device; Step S2: The heating block (607) is powered on, and the exhaust fan (104) is turned on to circulate air inside the device for ventilation; Step S3: Start the oil pump (407), and power on the heating rod (405) to gradually preheat the inside of the device to the specified temperature and then keep it constant. Step S4: After disinfection for a period of time, the ultraviolet light strip (504) is de-energized, and the supplemental light strip (503) is energized to emit light, waiting for subsequent supplemental lighting of the plants; Step S5: After opening the cabinet door body (201), place the culture bottle with the planted Bupleurum tissue on the shelf (105), then close the cabinet door body (201) and lock the cabinet lock; Step S6: When it is necessary to observe the growth of Bupleurum chinense from the outside, the electromagnet (302) is energized to draw out the black oily liquid from the liquid-conducting hollow glass plate (206) to ensure that the liquid-conducting hollow glass plate (206) is transparent to light; Step S7: After observation, the electromagnet (302) is de-energized, and the black oily liquid is pumped back into the liquid-conducting hollow glass plate (206) and filled, ensuring that the liquid-conducting hollow glass plate (206) is opaque.

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