Composition for improving sleep and memory, preparation method and application thereof

Abstract

The present invention provides a composition for improving sleep and memory, as well as a preparation method and application, and relates to the field of food technology. The composition of the present invention is made of the following components: 45-70 parts of compound berry, 5-11 parts of rose pollen, 3-5 parts of black tea powder, 5-10 parts of fish oil microcapsule powder, 5-10 parts of avocado powder, 3-8 parts of black wolfberry powder, 1.2-2.5 parts of tea theanine, 0.2-0.8 parts of γ-aminobutyric acid and 1-3 parts of oligoxylose; the compound berry is extracted by supercritical fluid extraction; the components are reasonably matched so that the functional factors can exert the overall comprehensive efficacy, achieve the significant effects of helping sleep, relieving stress and improving body fatigue, and the food prepared by the composition has the effects of improving sleep and improving memory.

Description

Technical Field

[0001] The invention belongs to the technical field of food, and specifically relates to a composition for improving sleep and memory, and a preparation method and application thereof. Background Art

[0002] Insomnia is a common sleep problem, with symptoms including difficulty falling asleep, waking up easily at night, waking up early or feeling unclear after waking up, which has adverse effects on people's physical and mental health. The daytime functional disorders caused by insomnia mainly include fatigue, depression or irritability, physical discomfort, cognitive impairment, memory loss, etc. There are many reasons for insomnia: poor sleep quality, excessive pressure in life or work, emotional tension, mental or physical illness, etc.

[0003] There are psychological treatment, drug treatment, and traditional Chinese medicine treatment for insomnia, among which taking sleeping pills is a common treatment method. However, taking sleeping pills has the problem of drug dependence. Based on this, people have conducted further research on food formulas in order to obtain a food that can calm the mind, improve sleep disorders, and improve memory.

[0004] Chinese invention patent CN112869162A discloses a composite herbal oligopeptide composition with anti-fatigue and sleep-aiding functions, including the following components by weight: 5-10 parts of γ-aminobutyric acid, 1-5 parts of L-theanine, 15-35 parts of Chinese herbal extract, 1-5 parts of saffron extract, 1-5 parts of passion fruit powder, 15-30 parts of composite oyster peptide microcapsules, 10-25 parts of wheat oligopeptides, 1-5 parts of casein hydrolyzed peptides, 1-5 parts of walnut peptides and 5-10 parts of Montmorency sour cherry powder; the raw materials of the Chinese herbal extract include stir-fried sour jujube seeds, Poria cocos, cinnamon, licorice and jujube. The composition of the invention organically combines high-quality oligopeptides with various functional ingredients, which can refresh the mind and be energetic during the day and fall asleep peacefully at night, giving full play to its comprehensive effects of improving sleep, relieving stress and resisting fatigue. However, a large amount of oligopeptides are used in the above composition, which is prone to produce unpleasant odor and poor taste; and the composition has complex ingredients, resulting in low storage stability.

[0005] Chinese invention patent CN103622003A discloses a kind of calming and sleeping conditioning meal or meal replacement product, the main components and preparation method of which are: taking jujube seeds, lotus seed core, mulberry, wolfberry, lotus seeds, tangerine peel, rose and Euryale ferox for crushing, mixing with longan powder, sunflower seed kernel powder, ganoderma lucidum powder, Poria powder, lily powder, litchi powder, oyster powder, lotus root powder, apple powder, red date powder, walnut kernel powder, black sesame powder, millet powder, yam powder and hawthorn powder, high temperature ripening, and then adding vitamin B group, biological clock regulating molecules, melatonin and soy isoflavones. The food uses the principle of physical balance in traditional Chinese medicine to restore the balance of the human body through dietary adjustment, adjusts the inherent bias of heart and liver blood deficiency, restores the overall function of the body, reduces the incidence of related diseases caused by heart and liver blood deficiency and heart yin deficiency, has small toxic and side effects and is cheap. However, the food has weak effects of calming the mind, helping sleep and relieving stress, and eating too much can easily cause drowsiness.

[0006] In addition, in foods that have the function of calming the nerves and improving sleep disorders, in order to improve the effect of calming the nerves and helping sleep, a large amount of melatonin, an ingredient with a calming effect, is often added. Although the effect of calming the nerves and helping sleep can be achieved, it is not suitable for daytime use. Taking it during the day will cause mental fatigue, which is not conducive to improving the quality of sleep at night. Existing foods with calming effect have complicated ingredients, containing a large number of calming ingredients, and a large number of anti-fatigue and nerve-stimulating ingredients, which easily cause conflicts between the effects of the ingredients, resulting in insomnia at night and fatigue during the day.

[0007] Therefore, there is an urgent need to provide a food that can relax emotions, relieve stress, calm nerves and help sleep, and can also improve body fatigue, invigorate the brain, and improve memory. Summary of the invention

[0008] In view of the problems existing in the prior art, the present invention provides a composition for improving sleep and memory, as well as a preparation method and application thereof. The present invention controls the dosage of rose pollen, tea theanine, black tea powder, blueberry powder, fish oil microcapsule powder, avocado powder, black wolfberry powder, aronia powder, gamma-aminobutyric acid and xylo-oligosaccharide components, and reasonably matches the components so that the functional factors can exert their overall comprehensive efficacy, thereby achieving the significant effects of helping sleep, relieving stress, improving body fatigue, and strengthening the brain and improving intelligence.

[0009] To achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0010] Firstly, the present invention provides a composition for improving sleep and memory, which is prepared from the following components by weight: 45-70 parts of compound berry, 5-11 parts of rose pollen, 3-5 parts of black tea powder, 5-10 parts of fish oil microcapsule powder, 5-10 parts of avocado powder, 3-8 parts of black wolfberry powder, 1.2-2.5 parts of tea theanine, 0.2-0.8 parts of gamma-aminobutyric acid and 1-3 parts of xylo-oligosaccharide;

[0011] The compound berry is composed of 40-60 parts of blueberry powder and 5-10 parts of aronia powder;

[0012] The compound berry extract is prepared from the compound berry, and the preparation method of the compound berry extract is as follows: supercritical fluid extraction is used for extraction, carbon dioxide with a purity of ≥99% is introduced, the critical temperature is 32-35°C, the critical pressure is 15-20MPa, the carbon dioxide flow rate is 20-32kg / h, and the extraction time is 2-4h.

[0013] Preferably, the composition is made of the following components in parts by weight: 45-55 parts of blueberry powder, 6-8 parts of wild aronia powder, 6-9 parts of rose pollen, 3.5-4.5 parts of black tea powder, 6.5-9 parts of fish oil microcapsule powder, 6.5-9 parts of avocado powder, 5.2-7 parts of black wolfberry powder, 1.5-2 parts of tea theanine, 0.4-0.6 parts of γ-aminobutyric acid and 1.5-2.5 parts of xylo-oligosaccharide.

[0014] Further preferably, the composition is made of the following components in parts by weight: 50 parts of blueberry powder, 7 parts of aronia powder, 8 parts of rose pollen, 4 parts of black tea powder, 8.5 parts of fish oil microcapsule powder, 8 parts of avocado powder, 6.6 parts of black wolfberry powder, 1.8 parts of tea theanine, 0.5 parts of γ-aminobutyric acid and 2 parts of xylo-oligosaccharide.

[0015] Preferably, the preparation method of the compound berry extract is: subjecting blueberry powder and aronia powder to low-temperature supercritical fluid extraction of carbon dioxide in a supercritical fluid extraction kettle, introducing carbon dioxide with a purity of ≥99% during the extraction process, controlling the critical temperature to 32-34°C, the critical pressure to 16.5-18MPa, the carbon dioxide flow rate to 22-28kg / h, the extraction time to 3-4h, decompressing the material, and drying to obtain the compound berry extract.

[0016] Further preferably, the preparation method of the compound berry extract is: subjecting blueberry powder and aronia powder to low-temperature supercritical fluid extraction of carbon dioxide in a supercritical fluid extraction kettle, introducing carbon dioxide with a purity of ≥99% during the extraction process, controlling the critical temperature to 33°C, the critical pressure to 17MPa, the carbon dioxide flow rate to 25kg / h, the extraction time to 3.5h, decompressing the material, and drying to obtain the compound berry extract.

[0017] Preferably, the weight ratio of the fish oil microcapsule powder, avocado powder, tea theanine and γ-aminobutyric acid is 6.5-9:6.5-9:1.5-2:0.4-0.6; further preferably, the weight ratio of the fish oil microcapsule powder, avocado powder, tea theanine and γ-aminobutyric acid is 8.5:8:1.8:0.5.

[0018] Preferably, the weight ratio of the blueberry powder, aronia powder, rose pollen, black tea powder and black wolfberry powder is 45-55:6-8:6-9:3.5-4.5:5.2-7; further preferably, the weight ratio of the blueberry powder, aronia powder, rose pollen, black tea powder and black wolfberry powder is 50:7:8:4:6.6.

[0019] Then, the present invention provides a preparation method of the above-mentioned composition, comprising the steps of: preparing a compound berry extract; uniformly mixing the obtained compound berry extract with rose pollen, black tea powder, fish oil microcapsule powder, avocado powder, black wolfberry powder, tea theanine, γ-aminobutyric acid and xylo-oligosaccharide to obtain a composition.

[0020] Preferably, the preparation method of the compound berry extract is: extraction by supercritical fluid extraction.

[0021] Further preferably, the conditions for supercritical fluid extraction are: introduction of carbon dioxide with a purity of ≥99%, a critical temperature of 32-35°C, a critical pressure of 15-20MPa, a carbon dioxide flow rate of 20-32kg / h, and an extraction time of 2-4h.

[0022] Further preferably, the preparation method of the compound berry extract is: subjecting blueberry powder and aronia powder to low-temperature supercritical fluid extraction of carbon dioxide in a supercritical fluid extraction kettle, introducing carbon dioxide with a purity of ≥99% during the extraction process, controlling the critical temperature to 32-34°C, the critical pressure to 16.5-18MPa, the carbon dioxide flow rate to 22-28kg / h, the extraction time to 3-4h, decompressing the material, and drying to obtain the compound berry extract.

[0023] Further preferably, the preparation method of the compound berry extract is: subjecting blueberry powder and aronia powder to low-temperature supercritical fluid extraction of carbon dioxide in a supercritical fluid extraction kettle, introducing carbon dioxide with a purity of ≥99% during the extraction process, controlling the critical temperature to 33°C, the critical pressure to 17MPa, the carbon dioxide flow rate to 25kg / h, the extraction time to 3.5h, decompressing the material, and drying to obtain the compound berry extract.

[0024] Finally, the present invention provides application of the above composition in food for improving sleep and memory.

[0025] Compared with the prior art, the present invention has the following beneficial effects:

[0026] 1. The composition of the present invention has significant effects of calming, strengthening the brain and improving intelligence, and delaying brain aging by synergistically matching the brain-benefitting functional substances of blueberry powder, fish oil microcapsule powder, avocado powder and γ-aminobutyric acid; and is combined with rose, black tea powder, black wolfberry, aronia powder and tea theanine ingredients to assist in relieving fatigue. The reasonable matching and synergistic effect of each ingredient can exert the overall comprehensive efficacy, achieving the effects of significantly helping sleep, relieving stress, improving body fatigue, and strengthening the brain and improving intelligence.

[0027] 2. The compound berry extract is prepared by supercritical fluid extraction. The compound berry extract has a high yield and high activity of effective ingredients. It has a significant effect in relieving stress and delaying brain aging. The compound berry extract synergizes with fish oil microcapsule powder, avocado powder, tea theanine and γ-aminobutyric acid to improve sleep quality, relieve anxiety and reduce fatigue. DETAILED DESCRIPTION

[0028] The following non-limiting examples can enable those skilled in the art to more fully understand the present invention, but do not limit the present invention in any way. The following content is merely an exemplary description of the scope of the present invention, and those skilled in the art can make various changes and modifications to the present invention based on the disclosed content, which should also fall within the scope of the present invention.

[0029] When the embodiments give numerical ranges, it should be understood that, unless otherwise specified in the present invention, both endpoints of each numerical range and any numerical value between the two endpoints can be selected. Unless otherwise defined, all technical and scientific terms used in the present invention have the same meanings as those generally understood by those of ordinary skill in the art to which the present invention belongs.

[0030] The present invention is further described below by way of specific examples. Unless otherwise specified, the various chemical reagents used in the examples of the present invention are obtained through conventional commercial channels.

[0031] In the following embodiments, the tea theanine was purchased from Shanghai Norde Biological Industry Co., Ltd. with the item number KL-NLTE024; the black tea powder was purchased from Hangzhou Mingbao Biotechnology Co., Ltd. with the item number MBB292; the fish oil microcapsule powder was purchased from Qingdao Haizhiyuan Life Science Co., Ltd. with the item number B04; the avocado powder was purchased from Tuofeng Health Technology (Nantong) Co., Ltd. with the item number SD1058; the γ-aminobutyric acid was purchased from Huaxi Biotechnology Co., Ltd. with the item number 120116HXSW003; the xylo-oligosaccharide was purchased from Shandong Bailong Chuangyuan Biotechnology Co., Ltd. with the item number XOS-95S.

[0032] For the above components, products from different manufacturers have no significant effect on the effects.

[0033] Example 1-Example 5

[0034] A composition that helps to relax emotions and relieve stress is prepared from the components shown in Table 1 in parts by weight:

[0035] Table 1

[0036]

[0037] In Example 1 to Example 5, a composite berry extract was prepared from blueberry powder and aronia powder: the blueberry powder and aronia powder were subjected to low-temperature supercritical fluid extraction of carbon dioxide in a supercritical fluid extraction kettle. During the extraction process, carbon dioxide with a purity of ≥99% was introduced, the critical temperature was controlled at 33°C, the critical pressure was 17MPa, the carbon dioxide flow rate was 25kg / h, the extraction time was 3.5h, the material was discharged under reduced pressure, and freeze-dried to obtain a composite berry extract.

[0038] The composition of Example 1 to Example 5 is prepared by uniformly mixing the compound berry extract with rose pollen, black tea powder, fish oil microcapsule powder, avocado powder, black wolfberry powder, tea theanine, γ-aminobutyric acid and xylo-oligosaccharide to obtain the composition.

[0039] Example 6

[0040] Different from Example 1, the preparation method of the compound berry extract is different.

[0041] In this embodiment, the preparation method of the compound berry extract is as follows: blueberry powder and wild aronia powder are subjected to low-temperature supercritical fluid extraction of carbon dioxide in a supercritical fluid extraction kettle. During the extraction process, carbon dioxide with a purity of ≥99% is introduced, the critical temperature is controlled to be 34°C, the critical pressure is 18MPa, the carbon dioxide flow rate is 22kg / h, the extraction time is 4h, the material is discharged under reduced pressure, the extract is filtered to obtain the extract, and the extract is freeze-dried to obtain the compound berry extract.

[0042] The rest are the same as in Example 1.

[0043] Comparative Example 1

[0044] The difference from Example 1 is that the components in the composition are different, specifically:

[0045] The composition is prepared from the following components in parts by weight: 8 parts of rose pollen, 4 parts of black tea powder, 0.5 parts of gamma-aminobutyric acid and 2 parts of xylo-oligosaccharide.

[0046] The rest are the same as in Example 1.

[0047] Comparative Example 2

[0048] The difference from Example 1 is that the components in the composition are different, specifically:

[0049] The composition is prepared from the following components in parts by weight: 50 parts of blueberry powder, 7 parts of aronia powder, 8.5 parts of fish oil microcapsule powder, 8 parts of avocado powder, 6.6 parts of black wolfberry powder, 1.8 parts of tea theanine and 2 parts of xylo-oligosaccharide.

[0050] The rest are the same as in Example 1.

[0051] Comparative Example 3

[0052] The difference from Example 1 is that the black tea powder is replaced with 0.01 parts of caffeine. The rest is the same as Example 1.

[0053] Comparative Example 4

[0054] The difference from Example 1 is that the aronia powder is replaced by cherry powder in the composition, and the extract is an extract of aronia powder and cherry powder. The rest is the same as Example 1.

[0055] Comparative Example 5

[0056] The difference from Example 1 is that the weight proportions of tea theanine and γ-aminobutyric acid in the composition are different, specifically:

[0057] The composition is made of the following components in parts by weight: 50 parts of blueberry powder, 7 parts of wild aronia powder, 8 parts of rose pollen, 4 parts of black tea powder, 8.5 parts of fish oil microcapsule powder, 8 parts of avocado powder, 6.6 parts of black wolfberry powder, 1.15 parts of tea theanine, 1.15 parts of gamma-aminobutyric acid and 2 parts of xylo-oligosaccharide.

[0058] The rest are the same as in Example 1.

[0059] Comparative Example 6

[0060] Different from Example 1, the preparation method of the compound berry extract is different, specifically:

[0061] The blueberry powder and the aronia berry powder were subjected to low-temperature supercritical fluid extraction of carbon dioxide in a supercritical fluid extraction kettle. During the extraction process, carbon dioxide with a purity of ≥99% was introduced, the critical temperature was 40°C, the critical pressure was 7MPa, the carbon dioxide flow rate was 10kg / h, the extraction time was 3.5h, the material was discharged under reduced pressure, the extract was filtered to obtain the extract, and the extract was freeze-dried to obtain a compound berry extract.

[0062] The rest are the same as in Example 1.

[0063] Comparative Example 7

[0064] The composition was prepared by referring to the raw material composition (IX) of Example 9 of Chinese invention patent CN113907349A.

[0065] The composition of Comparative Example 7 consists of the following raw materials in parts by weight: 10 parts of Chinese jujube kernel, 3 parts of wolfberry, 3 parts of malt, 3 parts of reed root, 5 parts of Poria cocos, 2 parts of licorice, 2 parts of 60% blueberry juice, 2 parts of 60% cherry juice, 2 parts of 60% rose juice, 1 part of tea theanine, 1 part of γ-aminobutyric acid, 0.4 parts of xylo-oligosaccharides, 0.4 parts of isomaltooligosaccharides, 0.4 parts of fructo-oligosaccharides, 0.4 parts of erythritol, 0.4 parts of isomaltulose, 0.5 parts of Lactobacillus plantarum, 0.5 parts of Lactobacillus acidophilus and 0.5 parts of Lactococcus lactis.

[0066] Experiment 1: Anti-fatigue, sedation and tranquilizing effect experiment

[0067] Experimental animals: Kunming mice, half male and half female, weighing 18-22g, were randomly divided into 14 groups, 7 mice in each group, production license number: SCXK (Zhejiang) 2022-0005. The model control group was gavaged with the same volume of normal saline; the example group was gavaged with 0.8g composition / kg; the comparative group was gavaged with 0.8g composition / kg. The drug was administered by gavage for 10 consecutive days, once a day.

[0068] Anti-fatigue efficacy test method (pole climbing test):

[0069] 30 minutes after the last administration, the mice were placed on a vertically suspended glass rod to make their muscles tense, and the time (s) it took for the mice to fall from the glass rod due to muscle fatigue was recorded, which was the climbing time. The experimental results are shown in Table 2.

[0070] Test method for tranquilizing effect (sleep time experiment):

[0071] After the pole climbing test, the mice were fed normally by gavage for 1 day, and the drug was administered once by gavage. 75 minutes after the last administration, 40 mg / kg of sodium pentobarbital was injected intraperitoneally. The sleeping time and awakening time of the mice were recorded. The awakening time (righting reflex recovery time) minus the sleeping time was the sleeping time of the mice. The experimental results are shown in Table 2.

[0072] Sedative efficacy test method (convulsion latency test):

[0073] After the sleep time experiment, the mice were fed normally by gavage for 5 days, with medication once a day. Then, 75 minutes after the last medication, 1.5 mg / kg of strychnine nitrate injection was injected intraperitoneally, and the convulsion latency of the mice was recorded. The experimental results are shown in Table 2.

[0074] Table 2

[0075]

[0076] In Table 2, each group was compared with the model control group: # P≤0.05, ##P≤0.01; Comparative Example group compared with Example 1 group: * P≤0.05, ** P≤0.01.

[0077] The results in Table 2 show that the composition of the present invention can significantly prolong the time for mice to climb a pole compared with the composition of the comparative example, with a significant difference. The composition of the present invention has a high anti-fatigue effect.

[0078] The results in Table 2 show that the composition of the present invention can prolong the sleeping time of mice compared with the composition of the comparative example, and has significant difference compared with the model group. The composition of the present invention has significantly improved tranquilizing and sleep-inducing effects.

[0079] The results in Table 2 show that the composition of the present invention can prolong the latency of convulsions caused by strychnine nitrate in mice compared with the composition of the comparative example, and has significant difference compared with the model group and the composition of the comparative example (P≤0.05). It can be seen that the composition of the present invention has strong anticonvulsant and sedative effects, and has significant effects of relaxing emotions and relieving stress.

[0080] In addition, the components in the compositions of Comparative Examples 1 and 2 are not the components of the present invention, and their effects of calming the nerves and improving sleep are significantly reduced; from the results of Comparative Examples 1 and 2, it can be seen that the components of the compositions of the present invention are indispensable and cooperate with each other to have a synergistic effect of calming the nerves and improving sleep.

[0081] Experiment 2: Brain-boosting and intelligence-enhancing effects

[0082] Experimental animals: SPF SD rats, 8 weeks old, half male and half female, adaptively raised for 1 week.

[0083] Experimental animal grouping: Rats were randomly divided into 9 groups, with 7 rats in each group. The model control group was gavaged with the same volume of physiological saline; the example group was gavaged with 0.8 g / kg of the composition of the corresponding example; the comparative example group was gavaged with 0.8 g / kg of the composition of the corresponding comparative example. The administration was continued by gavage for 14 days, once a day.

[0084] Experimental method: water maze method. The Morris water maze has a diameter of 1.65m, and the inner wall and bottom are completely black. The water maze area is divided into four quadrants. A black platform with a diameter of 12cm is placed 2cm below the water surface in the center of the second quadrant. After each water change in the water maze experiment, a certain amount of black dye is added to make the water black and opaque. The water temperature is maintained at 20±2℃ during the experiment.

[0085] On the 14th day after administration, a positioning navigation experiment was conducted. During the positioning navigation experiment, each rat was trained 4 times a day with an interval of 10 minutes. During training, a water entry point was randomly selected and the rat was placed in the water facing the pool wall. The rats entered the water from 4 different water entry points for 4 training sessions. The time required for each rat to find the underwater platform after entering the water from 4 different points, i.e., the latency to escape to the platform (escaping latency), was recorded. The animal was allowed to find the platform within 60 seconds and stay on the platform for 15 seconds after finding it. The average latency of the 4 water entry points on the 15th day of administration was used as the directional swimming result. The latency results of the water maze test for each group of rats are shown in Table 3.

[0086] During the water maze test, reference objects such as lights around the water maze remained unchanged to avoid environmental interference.

[0087] Table 3

[0088]

[0089] In Table 3, each group was compared with the model control group: # P≤0.05, ## P≤0.01; Comparative Example group compared with Example 1 group: * P≤0.05, ** P≤0.01.

[0090] As can be seen from Table 3, the composition of the present invention can significantly improve the memory of rats compared to the composition of the comparative example, and has a significant brain-boosting and intelligence-enhancing effect. In addition, it can be seen from the results of comparative examples 1 and 2 that the composition lacking the components has no significant difference in latency effect from the model control group, and even if the effects of comparative examples 1 and 2 are added together, the effect of improving memory described in the present invention cannot be achieved. The components of the composition of the present invention cooperate with each other, and have a synergistic effect of improving memory.

[0091] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions of the technical solution of the present invention by ordinary technicians in this field do not deviate from the essence and scope of the technical solution of the present invention.

Claims

1. A composition for improving sleep and memory, characterized in that: The invention is prepared from the following components by weight: 45-70 parts of compound berry, 5-11 parts of rose pollen, 3-5 parts of black tea powder, 5-10 parts of fish oil microcapsule powder, 5-10 parts of avocado powder, 3-8 parts of black wolfberry powder, 1.2-2.5 parts of tea theanine, 0.2-0.8 parts of gamma-aminobutyric acid and 1-3 parts of xylo-oligosaccharide; The compound berry is composed of 40-60 parts of blueberry powder and 5-10 parts of aronia powder; The compound berry extract is prepared from the compound berry, and the preparation method of the compound berry extract is as follows: supercritical fluid extraction is used for extraction, carbon dioxide with a purity of ≥99% is introduced, the critical temperature is 32-35°C, the critical pressure is 15-20MPa, the carbon dioxide flow rate is 20-32kg / h, and the extraction time is 2-4h.

2. The composition according to claim 1, characterized in that The invention is made of the following components by weight: 45-55 parts of blueberry powder, 6-8 parts of wild aronia powder, 6-9 parts of rose pollen, 3.5-4.5 parts of black tea powder, 6.5-9 parts of fish oil microcapsule powder, 6.5-9 parts of avocado powder, 5.2-7 parts of black wolfberry powder, 1.5-2 parts of tea theanine, 0.4-0.6 parts of γ-aminobutyric acid and 1.5-2.5 parts of xylo-oligosaccharide.

3. The composition according to claim 2, characterized in that The invention is made of the following components by weight: 50 parts of blueberry powder, 7 parts of wild aronia powder, 8 parts of rose pollen, 4 parts of black tea powder, 8.5 parts of fish oil microcapsule powder, 8 parts of avocado powder, 6.6 parts of black wolfberry powder, 1.8 parts of tea theanine, 0.5 parts of γ-aminobutyric acid and 2 parts of xylo-oligosaccharide.

4. The composition according to claim 1, characterized in that The preparation method of the compound berry extract comprises the following steps: subjecting blueberry powder and aronia powder to low-temperature supercritical fluid extraction of carbon dioxide in a supercritical fluid extraction kettle; during the extraction process, introducing carbon dioxide with a purity of ≥99%; controlling the critical temperature to be 32-34° C., the critical pressure to be 16.5-18 MPa, the carbon dioxide flow rate to be 22-28 kg / h, the extraction time to be 3-4 h, decompressing the material, discharging the material, and drying the material to obtain the compound berry extract.

5. The composition according to claim 4, characterized in that The preparation method of the compound berry extract comprises the following steps: subjecting blueberry powder and aronia powder to low-temperature supercritical fluid extraction of carbon dioxide in a supercritical fluid extraction kettle; during the extraction process, introducing carbon dioxide with a purity of ≥99%; controlling the critical temperature to be 33° C., the critical pressure to be 17 MPa, the carbon dioxide flow rate to be 25 kg / h, the extraction time to be 3.5 h, decompressing the material, discharging the material, and drying the material to obtain the compound berry extract.

6. The composition according to claim 1, characterized in that The weight ratio of the fish oil microcapsule powder, avocado powder, tea theanine and gamma-aminobutyric acid is 6.5-9:6.5-9:1.5-2:0.4-0.

6.

7. The composition according to claim 1, characterized in that The weight ratio of the blueberry powder, aronia powder, rose pollen, black tea powder and black wolfberry powder is 45-55:6-8:6-9:3.5-4.5:5.2-7.

8. The method for preparing the composition according to any one of claims 1 to 7, characterized in that: The method comprises the following steps: preparing a compound berry extract; and uniformly mixing the obtained compound berry extract with rose pollen, black tea powder, fish oil microcapsule powder, avocado powder, black wolfberry powder, tea theanine, gamma-aminobutyric acid and xylooligosaccharide to obtain a composition.

9. The preparation method according to claim 8, characterized in that: The preparation method of the compound berry extract is: extracting by supercritical fluid extraction, introducing carbon dioxide with a purity of ≥99%, a critical temperature of 32-35°C, a critical pressure of 15-20MPa, a carbon dioxide flow rate of 20-32kg / h, and an extraction time of 2-4h.

10. Use of the composition according to any one of claims 1 to 7 in food for improving sleep and memory.

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