A biopolymer film, its method of preparation and use in ophthalmic surgery

Abstract

The application relates to the technical field of ophthalmic material preparation, and particularly discloses a kind of biopolymer membranes, a preparation method thereof and application in ophthalmic surgery.The biopolymer membrane comprises the following raw material components by weight: polyvinyl alcohol 5-10 parts;gelatin 3-6 parts;sodium lignosulfonate 10-20 parts;carboxymethyl cellulose 3-6 parts;natural extract 1-3 parts;water 200-300 parts.Studies have shown that the biopolymer membrane can promote the proliferation of human corneal epithelial cells;therefore, it has important application value when used in ophthalmic surgery for the treatment of ocular surface damage.

Description

Technical Field

[0001] This invention relates to the field of ophthalmic material preparation technology, specifically to a biopolymer membrane, its preparation method, and its application in ophthalmic surgery. Background Technology

[0002] The amnion is a thin membrane composed of a single layer of interconnected epithelial cells; it is the innermost layer of the fetal membranes and is a semi-transparent membrane connected to the amniotic layer covering the placenta and umbilical cord. In clinical ophthalmology, the amnion can be used as a biomaterial to treat ocular surface injuries; it has significant application value. However, the amnion needs to be isolated and prepared from the fetal membranes, and the source of the raw material is inconvenient; therefore, improving the availability of a biopolymer membrane with readily available raw materials has important application value. Summary of the Invention

[0003] In order to solve at least one of the technical problems existing in the prior art, the present invention provides a biopolymer membrane, a method for preparing the same, and its application in ophthalmic surgery.

[0004] The technical solution of the present invention to solve the above-mentioned technical problems is as follows:

[0005] This invention first provides a biopolymer membrane comprising the following raw material components in parts by weight:

[0006] Polyvinyl alcohol 5-10 parts; gelatin 3-6 parts; sodium lignosulfonate 10-20 parts; carboxymethyl cellulose 3-6 parts; natural extract 1-3 parts; water 200-300 parts.

[0007] The inventors have demonstrated in their research that the biopolymer membrane prepared by this invention can promote the proliferation of human corneal epithelial cells and can be used to treat ocular surface injuries caused by corneal damage. In particular, the raw materials for preparing the biopolymer membrane of this invention are readily available and widely sourced, which is beneficial for the large-scale production of the biopolymer membrane.

[0008] Preferably, the biopolymer membrane comprises the following raw material components in parts by weight:

[0009] Polyvinyl alcohol 5-8 parts; gelatin 5-5 parts; sodium lignosulfonate 10-15 parts; carboxymethyl cellulose 3-5 parts; natural extract 2-3 parts; water 250-300 parts.

[0010] Most preferably, the biopolymer membrane comprises the following raw material components in parts by weight:

[0011] Polyvinyl alcohol 8 parts; gelatin 5 parts; sodium lignosulfonate 15 parts; carboxymethyl cellulose 5 parts; natural extract 2 parts; water 250 parts.

[0012] Preferably, the natural extract refers to a natural extract obtained from the traditional Chinese medicines safflower, Bletilla striata, and licorice.

[0013] Preferably, the natural extract is prepared by the following method:

[0014] After mixing the Chinese medicinal herbs safflower, bletilla striata and licorice evenly, the mixture is extracted with a solvent using ultrasound. After extraction, solid-liquid separation is performed, and the liquid is concentrated and dried to obtain the natural extract.

[0015] The inventors discovered in their research that the biopolymer membrane of the present invention promotes the proliferation of human corneal epithelial cells in a closely related manner with the addition of natural extracts. Studies have shown that adding natural extracts obtained from Chinese medicinal herbs such as safflower, Bletilla striata, and licorice to the biopolymer membrane of the present invention can significantly enhance the biopolymer membrane's effect on promoting the proliferation of human corneal epithelial cells.

[0016] Preferably, the weight ratio of the Chinese medicinal herbs safflower, bletilla striata, and licorice is 5-8:2-4:1-3;

[0017] The most preferred ratio is 7:3:2 for the traditional Chinese medicines safflower, bletilla striata and licorice.

[0018] Preferably, the ratio of the total weight of the Chinese medicinal herbs safflower, bletilla striata, and licorice to the amount of solvent is 1 kg: 6-12 L;

[0019] The most preferred ratio of the total weight of the Chinese medicinal herbs safflower, bletilla striata, and licorice to the amount of solvent is 1 kg: 8 L.

[0020] Preferably, the solvent is selected from one or a mixture of more than one of water, methanol, and acetone.

[0021] More preferably, the solvent is composed of water, methanol, and acetone.

[0022] Preferably, the volume ratio of water, methanol, and acetone is 1-3:4-6:1-3.

[0023] Most preferably, the volume ratio of water, methanol, and acetone is 2:5:2.

[0024] Further research by the inventors revealed that natural extracts obtained using different solvents exhibited varying degrees of effectiveness in promoting the proliferation of human corneal epithelial cells through biopolymer membranes. The study showed that natural extracts obtained using a solvent composed of water, methanol, and acetone significantly enhanced this effect compared to extracts obtained using only water, methanol, or acetone. Therefore, natural extracts obtained using a solvent composed of water, methanol, and acetone can synergistically enhance the effect of biopolymer membranes in promoting the proliferation of human corneal epithelial cells.

[0025] The present invention also provides a method for preparing the above-mentioned biopolymer membrane, which includes the following steps:

[0026] Polyvinyl alcohol, gelatin, sodium lignosulfonate, carboxymethyl cellulose and natural extracts are mixed evenly and then added to water. The mixture is heated to 40-60°C and stirred for 1-3 hours to obtain a biopolymer membrane dispersion.

[0027] The biopolymer membrane dispersion is coated onto the surface of a mold and dried to obtain the biopolymer membrane.

[0028] The present invention also provides an application of the above-mentioned biopolymer membrane in ophthalmic surgery.

[0029] Beneficial effects: This invention provides a novel biopolymer membrane; studies have shown that the biopolymer membrane described in this invention can promote the proliferation of human corneal epithelial cells; therefore, it has important application value in ophthalmic surgery for the treatment of ocular surface injuries. Detailed Implementation

[0030] The present invention will be further explained below with reference to specific embodiments, but the embodiments do not limit the scope of protection of the present invention.

[0031] Example 1: Preparation of Biopolymer Membranes

[0032] The raw material composition of the biopolymer membrane by weight is as follows: polyvinyl alcohol 8 parts; gelatin 5 parts; sodium lignosulfonate 15 parts; carboxymethyl cellulose 5 parts; natural extract 2 parts; water 250 parts;

[0033] The natural extract is prepared by the following method:

[0034] The Chinese medicinal herbs safflower, bletilla striata, and licorice were mixed evenly in a weight ratio of 7:3:2. The mixture was then ultrasonically extracted at 40°C for 30 minutes using a solvent. After extraction, solid-liquid separation was performed, and the liquid was concentrated and dried to obtain the natural extract. The solvent used was water.

[0035] Preparation method of biopolymer membrane: Polyvinyl alcohol, gelatin, sodium lignosulfonate, carboxymethyl cellulose and natural extract are mixed evenly and then added to water. The mixture is heated to 50°C and stirred for 2 hours to obtain a biopolymer membrane dispersion. The biopolymer membrane dispersion is coated on the surface of a mold and dried to obtain the biopolymer membrane.

[0036] Example 2: Preparation of Biopolymer Membranes

[0037] The raw material composition of the biopolymer membrane by weight is as follows: polyvinyl alcohol 8 parts; gelatin 5 parts; sodium lignosulfonate 15 parts; carboxymethyl cellulose 5 parts; natural extract 2 parts; water 250 parts;

[0038] The natural extract is prepared by the following method:

[0039] The Chinese medicinal herbs safflower, bletilla striata, and licorice were mixed evenly in a weight ratio of 7:3:2. The mixture was then ultrasonically extracted at 40°C for 30 minutes using a solvent. After extraction, solid-liquid separation was performed, and the liquid was concentrated and dried to obtain the natural extract. The solvent used was methanol.

[0040] Preparation method of biopolymer membrane: Polyvinyl alcohol, gelatin, sodium lignosulfonate, carboxymethyl cellulose and natural extract are mixed evenly and then added to water. The mixture is heated to 50°C and stirred for 2 hours to obtain a biopolymer membrane dispersion. The biopolymer membrane dispersion is coated on the surface of a mold and dried to obtain the biopolymer membrane.

[0041] Example 3: Preparation of Biopolymer Membranes

[0042] The raw material composition of the biopolymer membrane by weight is as follows: polyvinyl alcohol 8 parts; gelatin 5 parts; sodium lignosulfonate 15 parts; carboxymethyl cellulose 5 parts; natural extract 2 parts; water 250 parts;

[0043] The natural extract is prepared by the following method:

[0044] The Chinese medicinal herbs safflower, bletilla striata, and licorice were mixed evenly in a weight ratio of 7:3:2. The mixture was then ultrasonically extracted at 40°C for 30 minutes using a solvent. After extraction, solid-liquid separation was performed, and the liquid was concentrated and dried to obtain the natural extract. The solvent used was acetone.

[0045] Preparation method of biopolymer membrane: Polyvinyl alcohol, gelatin, sodium lignosulfonate, carboxymethyl cellulose and natural extract are mixed evenly and then added to water. The mixture is heated to 50°C and stirred for 2 hours to obtain a biopolymer membrane dispersion. The biopolymer membrane dispersion is coated on the surface of a mold and dried to obtain the biopolymer membrane.

[0046] Example 4: Preparation of Biopolymer Membranes

[0047] The raw material composition of the biopolymer membrane by weight is as follows: polyvinyl alcohol 8 parts; gelatin 5 parts; sodium lignosulfonate 15 parts; carboxymethyl cellulose 5 parts; natural extract 2 parts; water 250 parts;

[0048] The natural extract is prepared by the following method:

[0049] The Chinese medicinal herbs safflower, bletilla striata and licorice were mixed evenly in a weight ratio of 7:3:2. The mixture was then ultrasonically extracted at 40°C for 30 minutes using a solvent. After extraction, solid-liquid separation was performed, and the liquid was concentrated and dried to obtain the natural extract. The solvent consisted of water, methanol and acetone in a volume ratio of 2:5:2.

[0050] Preparation method of biopolymer membrane: Polyvinyl alcohol, gelatin, sodium lignosulfonate, carboxymethyl cellulose and natural extract are mixed evenly and then added to water. The mixture is heated to 50°C and stirred for 2 hours to obtain a biopolymer membrane dispersion. The biopolymer membrane dispersion is coated on the surface of a mold and dried to obtain the biopolymer membrane.

[0051] Example 5: Preparation of Biopolymer Membranes

[0052] The raw material composition of the biopolymer membrane by weight is as follows: polyvinyl alcohol 8 parts; gelatin 5 parts; sodium lignosulfonate 15 parts; carboxymethyl cellulose 5 parts; natural extract 2 parts; water 250 parts;

[0053] The natural extract is prepared by the following method:

[0054] The Chinese medicinal herbs safflower, bletilla striata, and licorice were mixed evenly in a weight ratio of 7:3:2. The mixture was then ultrasonically extracted at 40°C for 30 minutes using a solvent. After extraction, solid-liquid separation was performed, and the liquid was concentrated and dried to obtain the natural extract. The solvent consisted of water and methanol in a volume ratio of 2:5.

[0055] Preparation method of biopolymer membrane: Polyvinyl alcohol, gelatin, sodium lignosulfonate, carboxymethyl cellulose and natural extract are mixed evenly and then added to water. The mixture is heated to 50°C and stirred for 2 hours to obtain a biopolymer membrane dispersion. The biopolymer membrane dispersion is coated on the surface of a mold and dried to obtain the biopolymer membrane.

[0056] Example 6: Preparation of Biopolymer Membranes

[0057] The raw material composition of the biopolymer membrane by weight is as follows: polyvinyl alcohol 8 parts; gelatin 5 parts; sodium lignosulfonate 15 parts; carboxymethyl cellulose 5 parts; natural extract 2 parts; water 250 parts;

[0058] The natural extract is prepared by the following method:

[0059] The Chinese medicinal herbs safflower, bletilla striata, and licorice were mixed evenly in a weight ratio of 7:3:2. The mixture was then ultrasonically extracted at 40°C for 30 minutes using a solvent. After extraction, solid-liquid separation was performed, and the liquid was concentrated and dried to obtain the natural extract. The solvent consisted of water and acetone in a volume ratio of 1:1.

[0060] Preparation method of biopolymer membrane: Polyvinyl alcohol, gelatin, sodium lignosulfonate, carboxymethyl cellulose and natural extract are mixed evenly and then added to water. The mixture is heated to 50°C and stirred for 2 hours to obtain a biopolymer membrane dispersion. The biopolymer membrane dispersion is coated on the surface of a mold and dried to obtain the biopolymer membrane.

[0061] Example 7: Preparation of Biopolymer Membranes

[0062] The raw material composition of the biopolymer membrane by weight is as follows: polyvinyl alcohol 8 parts; gelatin 5 parts; sodium lignosulfonate 15 parts; carboxymethyl cellulose 5 parts; natural extract 2 parts; water 250 parts;

[0063] The natural extract is prepared by the following method:

[0064] The Chinese medicinal herbs safflower, bletilla striata, and licorice were mixed evenly in a weight ratio of 7:3:2. The mixture was then ultrasonically extracted at 40°C for 30 minutes using a solvent. After extraction, solid-liquid separation was performed, and the liquid was concentrated and dried to obtain the natural extract. The solvent consisted of methanol and acetone in a volume ratio of 5:2.

[0065] Preparation method of biopolymer membrane: Polyvinyl alcohol, gelatin, sodium lignosulfonate, carboxymethyl cellulose and natural extract are mixed evenly and then added to water. The mixture is heated to 50°C and stirred for 2 hours to obtain a biopolymer membrane dispersion. The biopolymer membrane dispersion is coated on the surface of a mold and dried to obtain the biopolymer membrane.

[0066] Comparative Example 1: Preparation of Biopolymer Membranes

[0067] The raw material composition of the biopolymer membrane by weight is as follows: polyvinyl alcohol 8 parts; gelatin 5 parts; sodium lignosulfonate 15 parts; carboxymethyl cellulose 5 parts; water 250 parts;

[0068] Preparation method of biopolymer membrane: Polyvinyl alcohol, gelatin, sodium lignosulfonate and carboxymethyl cellulose are mixed evenly and then added to water. The mixture is heated to 50°C and stirred for 2 hours to obtain a biopolymer membrane dispersion. The biopolymer membrane dispersion is coated on the surface of a mold and dried to obtain the biopolymer membrane.

[0069] The rate at which the biopolymer membranes prepared in Examples 1-7 and Comparative Example 1 promote the proliferation of human corneal epithelial cells is shown in Table 1.

[0070] Table 1. Experimental results on the promotion of human corneal epithelial cell proliferation rate by biopolymer membranes.

[0071]

[0072] As can be seen from the experimental results in Table 1, the proliferation rate of human corneal epithelial cells on the biopolymer membranes prepared in Examples 1-3 was significantly higher than that on the biopolymer membrane prepared in Comparative Example 1. This indicates that adding natural extracts obtained from Chinese medicinal herbs safflower, Bletilla striata, and licorice to the biopolymer membrane of the present invention can significantly enhance the biopolymer membrane's ability to promote the proliferation of human corneal epithelial cells.

[0073] As shown in Table 1, the proliferation rate of human corneal epithelial cells on the biopolymer membrane prepared in Example 4 was significantly higher than that on the biopolymer membranes prepared in Examples 1-3. This indicates that the natural extracts obtained by different solvent extraction methods have different effects on promoting the proliferation of human corneal epithelial cells on the biopolymer membrane. The study shows that the natural extracts obtained by solvent extraction using water, methanol, and acetone significantly enhance the effect of the biopolymer membrane on promoting the proliferation of human corneal epithelial cells compared to those obtained by extraction using only water, methanol, or acetone. The natural extracts obtained by solvent extraction using water, methanol, and acetone can synergistically enhance the effect of the biopolymer membrane on promoting the proliferation of human corneal epithelial cells.

[0074] As shown in Table 1, the proliferation rate of human corneal epithelial cells on the biopolymer membranes prepared in Examples 5-7 was not significantly higher than that on the biopolymer membranes prepared in Examples 1-3. This indicates that only natural extracts obtained by simultaneously using solvent extracts composed of water, methanol, and acetone can significantly enhance the effect of biopolymer membranes on promoting the proliferation of human corneal epithelial cells compared to natural extracts obtained by extracting with only water, methanol, or acetone; only then can the effect of biopolymer membranes on promoting the proliferation of human corneal epithelial cells be synergistically enhanced. However, natural extracts obtained by using solvent extracts composed of any two of water, methanol, and acetone cannot significantly enhance the effect of biopolymer membranes on promoting the proliferation of human corneal epithelial cells compared to natural extracts obtained by extracting with only water, methanol, or acetone.

Claims

1. A biopolymer membrane, characterized in that, The raw material components comprise the following parts by weight: Polyvinyl alcohol 5-10 parts; gelatin 3-6 parts; sodium lignosulfonate 10-20 parts; carboxymethyl cellulose 3-6 parts; natural extract 1-3 parts; water 200-300 parts; The natural extract is prepared by the following method: safflower, bletilla striata and licorice are mixed evenly in a weight ratio of 7:3:2, and then ultrasonically extracted with a solvent at 40°C for 30 minutes. After extraction, solid-liquid separation is performed, and the liquid is concentrated and dried to obtain the natural extract. The solvent is composed of water, methanol and acetone in a volume ratio of 2:5:

2.

2. The biopolymer membrane according to claim 1, characterized in that, The raw material components comprise the following parts by weight: Polyvinyl alcohol 5-8 parts; gelatin 5 parts; sodium lignosulfonate 10-15 parts; carboxymethyl cellulose 3-5 parts; natural extract 2-3 parts; water 250-300 parts.

3. The biopolymer membrane according to claim 1, characterized in that, The raw material components comprise the following parts by weight: Polyvinyl alcohol 8 parts; gelatin 5 parts; sodium lignosulfonate 15 parts; carboxymethyl cellulose 5 parts; natural extract 2 parts; water 250 parts.

4. The biopolymer membrane according to claim 1, characterized in that, The ratio of the total weight of the Chinese medicinal herbs safflower, bletilla striata, and licorice to the amount of solvent used is 1 kg: 6~12 L.

5. The biopolymer membrane according to claim 1, characterized in that, The ratio of the total weight of the Chinese medicinal herbs safflower, bletilla striata, and licorice to the amount of solvent used is 1 kg: 8 L.

6. The method for preparing the biopolymer membrane according to any one of claims 1 to 5, characterized in that, It includes the following steps: Polyvinyl alcohol, gelatin, sodium lignosulfonate, carboxymethyl cellulose and natural extracts are mixed evenly and then added to water. The mixture is heated to 40-60℃ and stirred for 1-3 hours to obtain a biopolymer membrane dispersion. The biopolymer membrane dispersion is coated onto the surface of a mold and dried to obtain the biopolymer membrane.

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