A biologically active citric acid-based polymer, and a method of preparing and using the same

Abstract

The application provides a kind of bioactive citric acid-based polymer and its preparation method and application, comprising: citric acid is melted under inert gas protection, and hydroxyl-containing bridging agent and hydroxyl-containing natural bioactive small molecule are added, and long-chain water-soluble hydroxyl-containing molecule is added simultaneously, and melt reaction is carried out under vacuum condition, and poly citric acid-octanediol-curcumin-polyethylene glycol polymer is obtained by reaction;Poly citric acid-octanediol-curcumin-polyethylene glycol polymer is purified and freeze-dried to obtain bioactive citric acid-based polymer.The preparation method of the application is simple, convenient to operate, low in cost, and suitable for large-scale production.The experimental results prove that the bioactive citric acid-based polymer prepared by the method has good biocompatibility in vivo and in vitro and good biological effect, can exhibit good antioxidant and anti-inflammatory performance, can eliminate inflammation and can promote tissue repair.

Description

Technical Field

[0001] This invention belongs to the field of biomedical materials technology, specifically relating to a bioactive citric acid-based polymer, its preparation method, and its application. Background Technology

[0002] Many diseases, such as rheumatoid arthritis, liver injury, and lung injury, are associated with inflammation. Inflammation is a defensive response to damage and harmful stimuli; however, excessive inflammation can lead to secondary tissue damage and dysfunction. The inflammatory process is often accompanied by the overexpression of pro-inflammatory factors and oxidative stress, which further exacerbate the inflammatory response. Anti-inflammatory therapy is generally considered an effective approach to treating inflammatory diseases. Clinical treatments include antibiotics and systemic steroid therapy, which aim to prevent bacterial infection but do not cure the disease. Furthermore, most of these drugs may have side effects, including cardiovascular disease and hepatotoxicity and nephrotoxicity. Developing innovative and effective strategies to modulate inflammation and treat related diseases is crucial. Compared to traditional treatments, bioactive polymer-based nanosystems have emerged as a promising strategy for modulating the inflammatory environment due to their ability to be manufactured on a large scale and their variable structure and function. To date, various polymer-based nanoparticles and nanovesicles have been developed and have played an active role in anti-inflammatory therapy. However, most reported polymer-based nanosystems exhibit problems such as non-biodegradability, poor bioactivity, and complex synthesis. Therefore, there is an urgent need to develop novel bioactive polymer nanosystems with simple synthesis methods, biodegradability, anti-inflammatory and antioxidant properties, and good biocompatibility to treat inflammatory diseases. In recent years, polycitrate (PC), as a typical bioactive synthetic polymer, has attracted much attention in regenerative medicine due to its low cost, biocompatibility, and biodegradability. PC-based polymers have been used to promote the regeneration of retinal, bone, muscle, and skin tissues. However, PC also has drawbacks such as low molecular weight, acidic degradation products, and insufficient bioactivity. To better expand the applications of functionalized PC, natural bioactive small molecules can be used as monomers to synthesize polymers with controllable activity. Many natural bioactive small molecules with hydroxyl groups, such as polyphenols, flavonoids, paclitaxel, and camptothecin, have anti-inflammatory activities similar to some anti-inflammatory drugs and show great promise in the field of inflammation treatment. However, these natural bioactive small molecules still have limitations such as low solubility, poor stability, low absorption rate, and low bioavailability. Developing polymers based on natural bioactive small molecules can not only overcome these limitations, but also enhance the anti-inflammatory bioactivity of citric acid-based polymers. Summary of the Invention

[0003] To address the problems of existing technologies, this invention provides a bioactive citric acid-based polymer, its preparation method, and its applications. The bioactive citric acid-based polymer prepared by this method exhibits good biocompatibility and beneficial biological effects both in vivo and in vitro.

[0004] To achieve the above objectives, the present invention employs the following technical solution:

[0005] The first objective of this invention is to provide a method for preparing a bioactive citric acid-based polymer. This method is simple, low-cost, and yields a polymer with good biocompatibility. It includes:

[0006] Citric acid was dissolved under an inert gas atmosphere, and hydroxyl-containing bridging agents and hydroxyl-containing natural bioactive small molecules were added. At the same time, long-chain water-soluble hydroxyl-containing molecules were added, and the reaction was carried out under vacuum to obtain polycitric acid-octanediol-curcumin-polyethylene glycol polymer.

[0007] The polycitric acid-octanediol-curcumin-polyethylene glycol polymer was purified, freeze-dried, and collected to obtain a bioactive citric acid-based polymer.

[0008] As a further improvement of the present invention, the hydroxyl-containing bridging agent is 1,8-octanediol.

[0009] As a further improvement of the present invention, the hydroxyl-containing natural bioactive small molecule is curcumin, catechin, resveratrol, proanthocyanidins, quercetin, anthocyanins, flavonoids or camptothecin.

[0010] As a further improvement of the present invention, the long-chain water-soluble hydroxyl-containing molecule is polyethylene glycol or polyoxyethylene-polyoxypropylene copolymer.

[0011] As a further improvement of the present invention, the molar ratio of citric acid, bridging agent, hydroxyl-containing natural bioactive small molecule and long-chain water-soluble hydroxyl-containing molecule is 1:(0.001-0.699):(0.001-0.699):(0.001-0.699).

[0012] As a further improvement of the present invention, the temperature of the melting reaction is 140-160°C and the reaction time is 2-5 hours.

[0013] As a further improvement of the present invention, the purified and lyophilized polycitric acid-octanediol-curcumin-polyethylene glycol polymer is collected, comprising:

[0014] Dissolve the PCGC polymer in water at low temperature, dialyze it using a dialysis bag for 2-3 days, freeze-dry the dialyzed product, and store it in a refrigerator.

[0015] A second objective of this invention is to address the important applications of the bioactive citric acid-based polymer in the treatment of inflammatory diseases and tissue repair. It is prepared using the described method. The synthesis method is simple and inexpensive.

[0016] Thirdly, the application of a bioactive citric acid-based polymer in the treatment of inflammatory diseases.

[0017] Fourthly, the application of a bioactive citric acid-based polymer in tissue repair dressings.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] This invention provides a method for preparing multifunctional poly(citric acid-octanediol-curcumin-polyethylene glycol) (PCGC) nanopolymers for treating inflammation and promoting tissue repair. The method utilizes citric acid, a hydroxyl-containing bridging agent, hydroxyl-containing natural bioactive small molecules, and polyethylene glycol as monomers, employing a one-pot thermal polymerization process to synthesize PCGC. The preparation method of this invention is simple, convenient, and low-cost, making it suitable for large-scale production. Experimental results demonstrate that the bioactive citric acid-based polymer prepared by this method exhibits good biocompatibility and biological effects both in vitro and in vivo, displaying excellent antioxidant and anti-inflammatory properties. It can alleviate inflammation and promote tissue repair, thus showing great promise for the treatment of inflammatory diseases and the promotion of tissue repair. Attached Figure Description

[0020] To more clearly illustrate the technical solutions in the specific embodiments of this application or the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this application. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0021] Figure 1 This is the structural formula of the bioactive citric acid-based polymer synthesized in this invention;

[0022] Figure 2 It is a bioactive citric acid-based polymer. 1 H NMR spectrum;

[0023] Figure 3 TEM image of the bioactive citrate-based polymer obtained in this invention;

[0024] Figure 4 The cytotoxicity of the bioactive citrate-based polymer prepared in this invention against fibroblasts (L929) was determined.

[0025] Figure 5 The in vitro antioxidant results of the bioactive citrate-based polymer obtained in this invention;

[0026] Figure 6 The in vitro anti-inflammatory results of the bioactive citric acid-based polymer prepared in this invention;

[0027] Figure 7 The results of in vivo treatment of acute lung injury with the bioactive citrate-based polymer (PCGC) prepared in this invention;

[0028] Figure 8 This is the result of in vivo treatment of inflammatory wounds with the bioactive citrate-based polymer (PCGC) prepared in this invention. Detailed Implementation

[0029] To make the technical problems, technical solutions, and beneficial effects of this application clearer, the following detailed description is provided in conjunction with embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the scope of this application.

[0030] In this application, the term "and / or" describes the relationship between related objects, indicating that three relationships can exist. For example, A and / or B can represent: A existing alone, A and B existing simultaneously, or B existing alone. A and B can be singular or plural. The character " / " generally indicates that the preceding and following related objects have an "or" relationship.

[0031] In this application, "at least one" means one or more, and "more than one" means two or more. "At least one of the following" or similar expressions refer to any combination of these items, including any combination of single or multiple items. For example, "at least one of a, b, or c", or "at least one of a, b, and c", can both mean: a, b, c, ab (i.e., a and b), ac, bc, or abc, where a, b, and c can be single or multiple.

[0032] It should be understood that in the various embodiments of this application, the order of the above processes does not imply the order of execution. Some or all steps may be executed in parallel or sequentially. The execution order of each process should be determined by its function and internal logic, and should not constitute any limitation on the implementation process of the embodiments of this application.

[0033] The terminology used in the embodiments of this application is for the purpose of describing particular embodiments only and is not intended to be limiting of this application. The singular forms “a,” “the,” and “the” used in the embodiments of this application and the appended claims are also intended to include the plural forms unless the context clearly indicates otherwise.

[0034] The weights of the relevant components mentioned in the embodiments of this application can refer not only to the specific content of each component, but also to the proportional relationship between the weights of the components. Therefore, any scaling up or down of the content of the relevant components according to the embodiments of this application is within the scope disclosed in the embodiments of this application. Specifically, the mass described in the embodiments of this application can be a mass unit known in the chemical industry, such as μg, mg, g, or kg.

[0035] The first objective of this invention is to provide a method for preparing a bioactive citric acid-based polymer, comprising:

[0036] Citric acid (PC) was dissolved under an inert gas atmosphere, and hydroxyl-containing bridging agents and hydroxyl-containing natural bioactive small molecules were added. At the same time, long-chain water-soluble hydroxyl-containing molecules were added, and the reaction was carried out under vacuum to obtain polycitric acid-octanediol-curcumin-polyethylene glycol polymer.

[0037] The polycitric acid-octanediol-curcumin-polyethylene glycol polymer (PCGC) was purified, lyophilized, and collected to obtain a bioactive citric acid-based polymer.

[0038] This invention proposes a multifunctional poly(octyl glycol citrate-curcumin-polyethylene glycol) (PCGC) nanopolymer for treating inflammation and promoting tissue repair. The method involves synthesizing PCGC via a one-pot thermal polymerization process using citric acid, a hydroxyl-containing bridging agent, a hydroxyl-containing natural bioactive small molecule, and polyethylene glycol as monomers. The preparation method of this invention is simple, convenient, and low-cost, making it suitable for large-scale production. Experimental results demonstrate that the bioactive citric acid-based polymer prepared by this method exhibits good biocompatibility and biological effects both in vitro and in vivo, displaying excellent antioxidant and anti-inflammatory properties. It can eliminate inflammation and promote tissue repair, thus showing great promise for the treatment of inflammatory diseases and the promotion of tissue repair.

[0039] Optionally, the hydroxyl-containing bridging agent is 1,8-octanediol (OD). Other hydroxyl-containing natural bioactive small molecules include curcumin (CUR), catechins, resveratrol, proanthocyanidins, quercetin, anthocyanins, flavonoids, and camptothecin. Long-chain water-soluble hydroxyl-containing polyethylene glycol (PEG) 200-10000 and polyoxyethylene-polyoxypropylene copolymer (F127) are also included.

[0040] As a further preferred option, the molar ratio of citric acid, bridging agent, hydroxyl-containing natural bioactive small molecule, and long-chain water-soluble hydroxyl-containing molecule is 1:(0.001-0.699):(0.001-0.699):(0.001-0.699), the reaction temperature is 140-160℃, and the reaction time is 2-5 hours.

[0041] In the examples, the molar ratio of citric acid, bridging agent, hydroxyl-containing natural bioactive small molecule, and long-chain water-soluble hydroxyl-containing molecule is 1:(0.001-0.699):(0.001-0.699):(0.001-0.699). Further ratios are: 1:(0.001-0.5):(0.001-0.5):(0.001-0.5), 1:(0.1-0.6):(0.1-0.6):(0.1-0.6), specifically: 1:0.001:0.001:0.001, 1:0.05:0.05:0.05, 1:0.1:0.1:0.1, 1:0.2. The ratios can be any combination of 0.2:0.2, 1:0.3:0.3:0.3, 1:0.4:0.4:0.4, 1:0.5:0.5:0.5, 1:0.6:0.6:0.6, 1:0.699:0.699:0.699, 1:0.1:0.2:0.3, 1:0.5:0.4:0.2, 1:0.4:0.6:0.2, etc. The specific dosage can be selected according to actual needs.

[0042] In the embodiments, the reaction temperature is 140-160℃ and the reaction time is 2-5 hours, further: 140-150℃, reaction time is 2-4 hours; 150-160℃, reaction time is 3-5 hours; 145-155℃, reaction time is 2.5-4.5 hours; specifically: 140℃, reaction time is 4 hours; 145℃, reaction time is 3 hours; 150℃, reaction time is 5 hours; 155℃, reaction time is 2 hours; 160℃, reaction time is 3.5 hours.

[0043] In the embodiments, the PCGC polymer also needs to be post-processed. The post-processing method is as follows: after the reaction is completed, the PCGC polymer is dissolved in water at low temperature, dialyzed with a dialysis bag for 2 to 3 days, and the dialyzed PCGC polymer is freeze-dried and stored in a refrigerator.

[0044] The second objective of this invention is to provide a bioactive citric acid-based polymer, which is prepared by the above method. The synthesis method is simple and low-cost, and it is obtained by the preparation method described above.

[0045] The present invention will now be described in further detail with reference to the accompanying drawings:

[0046] This invention discloses a method for preparing a bioactive citric acid-based polymer, comprising the following steps:

[0047] 1) First, heat 1 mol of citric acid at 160°C in an inert gas atmosphere to melt the citric acid. Then add 0.001-0.699 mol of 1,8-octanediol. After the 1,8-octanediol melts, add 0.001-0.699 mol of curcumin and 0.001-0.699 mol of polyethylene glycol 1000. Then, under vacuum conditions, maintain the temperature at 140°C for 2-5 hours to react.

[0048] 2) The citric acid polymer was dissolved in water at room temperature, and the resulting citric acid groups were purified using a dialysis tube (3.5 kDa) for 2–3 days. After freeze-drying, the citric acid groups were collected and stored to obtain the bioactive citric acid polymer.

[0049] This invention aims to prepare a bioactive citric acid-based polymer with good biocompatibility, capable of treating inflammatory diseases and promoting tissue repair.

[0050] Hydroxyl-containing natural bioactive small molecules possess safety, non-toxicity, and anti-inflammatory and antioxidant activities, attracting widespread interest from researchers and being used in various biomedical applications. Citric acid and its polymers have the advantages of simple degradation products, low cost, and good biocompatibility.

[0051] Therefore, in this invention, hydroxyl-containing natural bioactive small molecules are grafted onto a citric acid-based polymer to obtain a bioactive citric acid-based polymer. The bioactive citric acid-based polymer prepared by this method has good biocompatibility and good biological effects both in vivo and in vitro. It can exhibit good antioxidant and anti-inflammatory properties, reduce inflammation, and promote tissue repair. Therefore, this polymer has good application prospects in the treatment of inflammatory diseases and in tissue repair dressings.

[0052] To better understand the present invention, the present invention will be described in detail below with reference to specific embodiments, but the content of the present invention is not limited to the following embodiments.

[0053] Example 1

[0054] 1) First, heat 1 mol of citric acid at 160°C in an inert gas atmosphere to melt the citric acid. Then add 0.693 mol of 1,8-octanediol. After the 1,8-octanediol melts, add 0.007 mol of curcumin and 0.3 mol of polyethylene glycol 1000. Then, under vacuum conditions, maintain the temperature at 140°C for 5 hours to react.

[0055] 2) The citric acid polymer was dissolved in water at room temperature, and the resulting citric acid groups were purified using a dialysis tube (3.5 kDa) for 2–3 days. After freeze-drying, the citric acid groups were collected and stored to obtain the bioactive citric acid polymer.

[0056] The bioactive citric acid-based polymer prepared by the method of this invention exhibits good biocompatibility and anti-inflammatory properties, making it a polymer suitable for treating inflammatory diseases and promoting tissue repair. The following is a detailed analysis based on experimental data.

[0057] Figure 1 This is the structural formula of the bioactive citric acid-based polymer synthesized in this invention.

[0058] Figure 2 It is a bioactive citric acid-based polymer. 1 In the 1H NMR spectrum, citric acid exhibits multiple characteristic peaks (–CH2–) at 2.6–3.0 ppm, while 1.2, 1.5, 3.9, and 4.1 ppm represent the methylene (–CH2–) absorption peaks of 1,8-octanediol. The peaks at 7–8 ppm represent the hydrogen on the benzene ring of curcumin and the representative methylene (–CH2–) peaks. The methylene (–CH2–) characteristic peaks of polyethylene glycol appear at 3.5, 4.2, and 4.3 ppm.

[0059] Figure 3 The image shows a TEM image of the bioactive citric acid-based polymer obtained in this invention. As can be seen from the image, PCGC consists of nanoparticles with a diameter of approximately 40 nm.

[0060] Figure 4 The figure shows the cytotoxicity of the bioactive citrate-based polymer prepared in this invention to fibroblasts (L929). As can be seen from the figure, the bioactive citrate-based polymer exhibits good biocompatibility.

[0061] Figure 5 The in vitro antioxidant capacity of the bioactive citric acid-based polymer prepared in this invention shows that the bioactive citric acid-based polymer has a good scavenging effect on free radicals.

[0062] Figure 6 The figure shows the in vitro anti-inflammatory results of the bioactive citric acid-based polymer prepared in this invention. As can be seen from the figure, the bioactive citric acid-based polymer can downregulate the expression of pro-inflammatory factors and exhibits good anti-inflammatory ability.

[0063] A third objective of this invention is to provide the preparation method described above for the application of bioactive citrate-based polymers in the treatment of inflammatory diseases and in tissue repair dressings.

[0064] Figure 7The figure shows the results of in vivo treatment of acute lung injury with the bioactive citric acid-based polymer prepared in this invention. As can be seen from the figure, after treatment, lung tissue edema was significantly reduced, which was reflected in the reduction of lung volume.

[0065] Figure 8 The figure shows the in vivo results of promoting inflammatory wound healing with the bioactive citric acid-based polymer prepared in this invention. As can be seen from the figure, the wound area was significantly reduced after treatment.

[0066] Therefore, the bioactive citric acid-based polymer of the present invention can meet the potential for application in inflammatory disease treatment drugs and tissue repair-promoting dressings, and the effect is obvious.

[0067] This bioactive citric acid-based polymer exhibits good biocompatibility and biological effects both in vivo and in vitro. It demonstrates excellent antioxidant and anti-inflammatory properties, can reduce inflammation, and can promote tissue repair. Therefore, this polymer has great application prospects in the treatment of inflammatory diseases and in promoting tissue repair.

[0068] Example 2

[0069] 1) First, heat 1 mol of citric acid at 160°C in an inert gas atmosphere to melt the citric acid. Then add 0.686 mol of 1,8-octanediol. After the 1,8-octanediol melts, add 0.014 mol of curcumin and 0.3 mol of polyethylene glycol 1000. Then, under vacuum conditions, maintain the temperature at 140°C for 5 hours to react.

[0070] 2) The citric acid polymer was dissolved in water at room temperature, and the resulting citric acid groups were purified using a dialysis tube (3.5 kDa) for 2–3 days. After freeze-drying, the citric acid groups were collected and stored to obtain the bioactive citric acid polymer.

[0071] Example 3

[0072] 1) First, heat 1 mol of citric acid at 160°C in an inert gas atmosphere to melt the citric acid. Then add 0.665 mol of 1,8-octanediol. After the 1,8-octanediol melts, add 0.035 mol of curcumin and 0.3 mol of polyethylene glycol 1000. Then, under vacuum conditions, maintain the temperature at 140°C for 5 hours to react.

[0073] 2) The citric acid polymer was dissolved in water at room temperature, and the resulting citric acid groups were purified using a dialysis tube (3.5 kDa) for 2–3 days. After freeze-drying, the citric acid groups were collected and stored to obtain the bioactive citric acid polymer.

[0074] Example 4

[0075] 1) First, heat 1 mol of citric acid at 160°C in an inert gas atmosphere to melt the citric acid. Then add 0.693 mol of 1,8-octanediol. After the 1,8-octanediol melts, add 0.007 mol of curcumin and 0.3 mol of polyethylene glycol 400. Then, under vacuum conditions, maintain the temperature at 140°C for 5 hours to react.

[0076] 2) The citric acid polymer was dissolved in water at room temperature, and the resulting citric acid groups were purified using a dialysis tube (3.5 kDa) for 2–3 days. After freeze-drying, the citric acid groups were collected and stored to obtain the bioactive citric acid polymer.

[0077] Example 5

[0078] 1) First, heat 1 mol of citric acid at 160°C in an inert gas atmosphere to melt the citric acid. Then add 0.693 mol of 1,8-octanediol. After the 1,8-octanediol melts, add 0.007 mol of curcumin and 0.3 mol of polyethylene glycol 200. Then, under vacuum conditions, maintain the temperature at 140°C for 5 hours to react.

[0079] 2) The citric acid polymer was dissolved in water at room temperature, and the resulting citric acid groups were purified using a dialysis tube (3.5 kDa) for 2–3 days. After freeze-drying, the citric acid groups were collected and stored to obtain the bioactive citric acid polymer.

[0080] The bioactive citric acid-based polymer (PCGC) prepared in this invention has a simple preparation method, uses environmentally friendly raw materials, and is inexpensive. It can exhibit good anti-inflammatory properties and has a promising application prospect in the treatment of inflammatory diseases and the promotion of tissue repair.

[0081] The present invention also has the following advantages:

[0082] 1) The citric acid used in this invention is an important component produced by the mitochondrial tricarboxylic acid cycle. It has simple degradation components in vivo, is inexpensive, highly reactive, and has good biocompatibility.

[0083] 2) Curcumin used in this invention is a natural bioactive small molecule that exhibits excellent bioactivity in terms of antioxidation, anti-inflammation and regeneration.

[0084] 3) The bioactive citric acid-based polymer prepared in this invention has a simple preparation method, can be obtained in one step, and can be mass-produced on a large scale.

[0085] 4) The bioactive citric acid polymer (PCGC) prepared in this invention has anti-inflammatory and antioxidant capabilities.

[0086] 5) The solvent used in this invention is deionized water, and the bioactive citric acid polymer (PCGC) prepared by this method does not contain any organic solvents.

[0087] In summary, this invention proposes a multifunctional poly(citric acid-polyethylene glycol-curcumin) (PCGC) nanopolymer for treating inflammation and promoting tissue repair. The method synthesizes PCGC via a one-pot thermal polymerization process using citric acid, a hydroxyl-containing bridging agent, a hydroxyl-containing natural bioactive small molecule, and polyethylene glycol as monomers. The preparation method of this invention is simple, convenient, and low-cost, making it suitable for large-scale production.

[0088] The above content is only for illustrating the technical concept of the present invention and should not be construed as limiting the scope of protection of the present invention. Any modifications made to the technical solution based on the technical concept proposed in this invention shall fall within the scope of protection of the claims of this invention.

Claims

1. A method for preparing a bioactive citric acid-based polymer, characterized in that, include: Citric acid was dissolved under an inert gas atmosphere, and 1,8-octanediol, a hydroxyl-containing bridging agent, and curcumin, a hydroxyl-containing natural bioactive small molecule, were added. At the same time, long-chain water-soluble hydroxyl-containing molecules were added, and the reaction was carried out under vacuum to obtain a polycitric acid-octanediol-curcumin-polyethylene glycol polymer. The polycitric acid-octanediol-curcumin-polyethylene glycol polymer was purified, freeze-dried, and collected to obtain a bioactive citric acid-based polymer. The molar ratio of citric acid, bridging agent, hydroxyl-containing natural bioactive small molecule, and long-chain water-soluble hydroxyl-containing molecule is 1:(0.001-0.699):(0.001-0.699):(0.001-0.699). The melting reaction temperature is 140-160 ℃, and the reaction time is 2-5 hours; The long-chain water-soluble hydroxyl-containing molecule is polyethylene glycol 200-10000.

2. The method for preparing the bioactive citric acid-based polymer according to claim 1, characterized in that, The purified lyophilized polycitric acid-octyl glycol-curcumin-polyethylene glycol polymer was collected, including: The polycitric acid-octyl glycol-curcumin-polyethylene glycol polymer was dissolved in water at low temperature, dialyzed for 2-3 days using a dialysis bag, and then freeze-dried and stored in a refrigerator.

3. A bioactive citric acid-based polymer, characterized in that, It is prepared by the preparation method according to any one of claims 1 to 2.

4. The application of the bioactive citric acid-based polymer prepared by the preparation method according to any one of claims 1 to 2, characterized in that, Application in drugs for the treatment of inflammatory diseases.

5. The application of the bioactive citric acid-based polymer prepared by the preparation method according to any one of claims 1 to 2, characterized in that, Application in tissue repair dressings.

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